MC03g_new0326 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0326
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Non-specific serine/threonine protein kinase
Location	MC03: 14233192 .. 14235305 (-)
RNA-Seq Expression	MC03g_new0326
Synteny	MC03g_new0326

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTACTTTTAGGCAATCCTAAATTTACTGGGTCACCTTCCTGTTGTTCTGTTCTGTTAGGCTCTCCCAATTCTCAGCCACAAGAACAATCCAAAACCCAAAATCCACTCTCCCCTTCTACCAAAATTTTTGTAAAATTCTTCCCTTTTTCTCACCTCTCACCACGGGTCTCTCTCCCTCATCAACCCTGCCTCTTTAGGCACGGTTGAACTTCATTCTTCATTCTTCATTCTTCAACTTCAAATATGCTCCTCTCTCTCTGACTCCATCCAACCCATCAATACTTCTTCCTCTGTCCTCAAACATGGAGAAAAAGGGTGCCATTCTGATGCAGAGGTACGAGCTCGGGAGGTTGCTTGGGCAGGGGACCTTTGCCAAAGTGTACCATGCCCGGAACCTCCACACGGGCCAGAGCGTGGCCATCAAAATCATTGACAAGGAGAAGGTTCTGCGTGTGGGGCTCATCGACCAAATCAAGAGGGAGATCTCTGTGATGCGCCTCGTGCGCCACCCCAATGTGGTGCAGCTCTACGAGGTGATGGCCAGCAGGACCAAGATCTACTTCGTCATGGAGTATGTCAAGGGTGGGGAACTCTTCAACAAAGTGGCTAAGGGGAGGCTCAAGGAAGACGCCGCTCGTAAGTACTTCCAGCAGCTCATTGGCGCTGTTGATTACTGCCATAGCCGCGGAGTCTACCACAGAGATCTCAAGCCTGAGAATCTCCTTCTTGACGAATTTGGGAATCTCAAGATTTCGGATTTCGGATTGAGTGCGTTGGCCGAGTCTCGGCGGCAGGATGGCCTTCTCCATACCACCTGTGGAACCCCTGCCTATGTCGCCCCTGAAGTCATCAACAAGAAAGGTCTCATTTTTCCTCTTCTTATGCTGATTCTGTATTTGGTTACATAACCTTGCATATAGGATTGTAATTAATTCATCTTCTTTGGCATTTGGCAATTACTTTGTTTCACAGGTTATGATGGTGCCAAAGCTGATATTTGGTCTTCTGGGGTTGTCCTGTTTGTTCTGCTGGCGGGTTTCCTCCCATTCCACGACCCAAATCTGATGGAAATGTACAGGAGAATTTCCAGAGGAGAATTCAAATGCCCTCAATGGTTTCCTCCCGAAGTGCGCAAGCTTCTCTCAAGAATCCTCGACCCAAGTCCCCACACCAGAATAAACATGGCCAAACTCATGGACAATTCTTGGTTCAAGAAGGGCTTCAAAGCCAATGTAGCAACTCCCAAACCACTTCGAATCAATGGAGATGTGTCGTCATCGGTAAGCGACGTCCACGCCGCTTTCGACGACATTAGTTCCGAGGAAGGGTCGATTAATGGCAATAACAATGAGAGCGGAAAGCCACCGACCAGAAACTCTAGTTTCAATGCCTTTGACATCATATCTCTGTCGCCGGGGTTCGACCTGTCGGGTCTGTTCGAGAGCGACGTGGATCAGAGGCTGCAGTCGAGATTCACGACCGCGAAACCAGCATCGGCGATCGTGTCGAAGTTCGAGGAAATAGCGGAGATGGAGAGGTTCAAGGTGTTGAAGAAGGATGGGATAGTGAAACTGCAGGGGAGTAAACAGGGGAGGAAAGGGGAGCTGGCTATAGATGCTGAGATATTTGAAGTGACACCTTCTTTTTTTGTGGTGGAGGTGAAGAAAACTGCTGGGGACACTTTGGAATACAGGCAATTTTACAACAAAGACATAAGGCCTTGCCTCAAGGATATTGTGTGGACTTGGGAAGGAAATGAGCAGCAGCAGCAACAGCAACCACAACCACAAATTGTTTAGGCTGTTTCTTCTCAGTTCTCTCGTTAGAAATTAATATTAGAAAAAAGAAGGCTGTTTTTTCTTACTTGTTCCTATTCTTACCATTGCTTTTTCTCTATTCCCCATATTGATTGTATAATCATTCTGATCTCATTAATCTCTTCATTCTCTTGTTCAACTTCTTGGTTCTCTTGGAATCTAACTCAGAGATGGAGATTGTGATTAAAGAAAAAAAGGGACAAAAAAAATTGAACACCCATTTGTTGGTGTTCGTGGAATGGAATTCCAGGTGTGAAACAGTGAAAAGGCGATTACTTTTTAGTTGGTTTGTGATT

mRNA sequence

ATGGAGAAAAAGGGTGCCATTCTGATGCAGAGGTACGAGCTCGGGAGGTTGCTTGGGCAGGGGACCTTTGCCAAAGTGTACCATGCCCGGAACCTCCACACGGGCCAGAGCGTGGCCATCAAAATCATTGACAAGGAGAAGGTTCTGCGTGTGGGGCTCATCGACCAAATCAAGAGGGAGATCTCTGTGATGCGCCTCGTGCGCCACCCCAATGTGGTGCAGCTCTACGAGGTGATGGCCAGCAGGACCAAGATCTACTTCGTCATGGAGTATGTCAAGGGTGGGGAACTCTTCAACAAAGTGGCTAAGGGGAGGCTCAAGGAAGACGCCGCTCGTAAGTACTTCCAGCAGCTCATTGGCGCTGTTGATTACTGCCATAGCCGCGGAGTCTACCACAGAGATCTCAAGCCTGAGAATCTCCTTCTTGACGAATTTGGGAATCTCAAGATTTCGGATTTCGGATTGAGTGCGTTGGCCGAGTCTCGGCGGCAGGATGGCCTTCTCCATACCACCTGTGGAACCCCTGCCTATGTCGCCCCTGAAGTCATCAACAAGAAAGGTTATGATGGTGCCAAAGCTGATATTTGGTCTTCTGGGGTTGTCCTGTTTGTTCTGCTGGCGGGTTTCCTCCCATTCCACGACCCAAATCTGATGGAAATGTACAGGAGAATTTCCAGAGGAGAATTCAAATGCCCTCAATGGTTTCCTCCCGAAGTGCGCAAGCTTCTCTCAAGAATCCTCGACCCAAGTCCCCACACCAGAATAAACATGGCCAAACTCATGGACAATTCTTGGTTCAAGAAGGGCTTCAAAGCCAATGTAGCAACTCCCAAACCACTTCGAATCAATGGAGATGTGTCGTCATCGGTAAGCGACGTCCACGCCGCTTTCGACGACATTAGTTCCGAGGAAGGGTCGATTAATGGCAATAACAATGAGAGCGGAAAGCCACCGACCAGAAACTCTAGTTTCAATGCCTTTGACATCATATCTCTGTCGCCGGGGTTCGACCTGTCGGGTCTGTTCGAGAGCGACGTGGATCAGAGGCTGCAGTCGAGATTCACGACCGCGAAACCAGCATCGGCGATCGTGTCGAAGTTCGAGGAAATAGCGGAGATGGAGAGGTTCAAGGTGTTGAAGAAGGATGGGATAGTGAAACTGCAGGGGAGTAAACAGGGGAGGAAAGGGGAGCTGGCTATAGATGCTGAGATATTTGAAGTGACACCTTCTTTTTTTGTGGTGGAGGTGAAGAAAACTGCTGGGGACACTTTGGAATACAGGCAATTTTACAACAAAGACATAAGGCCTTGCCTCAAGGATATTGTGTGGACTTGGGAAGGAAATGAGCAGCAGCAGCAACAGCAACCACAACCACAAATTGTTTAG

Coding sequence (CDS)

ATGGAGAAAAAGGGTGCCATTCTGATGCAGAGGTACGAGCTCGGGAGGTTGCTTGGGCAGGGGACCTTTGCCAAAGTGTACCATGCCCGGAACCTCCACACGGGCCAGAGCGTGGCCATCAAAATCATTGACAAGGAGAAGGTTCTGCGTGTGGGGCTCATCGACCAAATCAAGAGGGAGATCTCTGTGATGCGCCTCGTGCGCCACCCCAATGTGGTGCAGCTCTACGAGGTGATGGCCAGCAGGACCAAGATCTACTTCGTCATGGAGTATGTCAAGGGTGGGGAACTCTTCAACAAAGTGGCTAAGGGGAGGCTCAAGGAAGACGCCGCTCGTAAGTACTTCCAGCAGCTCATTGGCGCTGTTGATTACTGCCATAGCCGCGGAGTCTACCACAGAGATCTCAAGCCTGAGAATCTCCTTCTTGACGAATTTGGGAATCTCAAGATTTCGGATTTCGGATTGAGTGCGTTGGCCGAGTCTCGGCGGCAGGATGGCCTTCTCCATACCACCTGTGGAACCCCTGCCTATGTCGCCCCTGAAGTCATCAACAAGAAAGGTTATGATGGTGCCAAAGCTGATATTTGGTCTTCTGGGGTTGTCCTGTTTGTTCTGCTGGCGGGTTTCCTCCCATTCCACGACCCAAATCTGATGGAAATGTACAGGAGAATTTCCAGAGGAGAATTCAAATGCCCTCAATGGTTTCCTCCCGAAGTGCGCAAGCTTCTCTCAAGAATCCTCGACCCAAGTCCCCACACCAGAATAAACATGGCCAAACTCATGGACAATTCTTGGTTCAAGAAGGGCTTCAAAGCCAATGTAGCAACTCCCAAACCACTTCGAATCAATGGAGATGTGTCGTCATCGGTAAGCGACGTCCACGCCGCTTTCGACGACATTAGTTCCGAGGAAGGGTCGATTAATGGCAATAACAATGAGAGCGGAAAGCCACCGACCAGAAACTCTAGTTTCAATGCCTTTGACATCATATCTCTGTCGCCGGGGTTCGACCTGTCGGGTCTGTTCGAGAGCGACGTGGATCAGAGGCTGCAGTCGAGATTCACGACCGCGAAACCAGCATCGGCGATCGTGTCGAAGTTCGAGGAAATAGCGGAGATGGAGAGGTTCAAGGTGTTGAAGAAGGATGGGATAGTGAAACTGCAGGGGAGTAAACAGGGGAGGAAAGGGGAGCTGGCTATAGATGCTGAGATATTTGAAGTGACACCTTCTTTTTTTGTGGTGGAGGTGAAGAAAACTGCTGGGGACACTTTGGAATACAGGCAATTTTACAACAAAGACATAAGGCCTTGCCTCAAGGATATTGTGTGGACTTGGGAAGGAAATGAGCAGCAGCAGCAACAGCAACCACAACCACAAATTGTTTAG

Protein sequence

MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIGAVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVRKLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDISSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV

Homology

[Close]

BLAST of MC03g_new0326 vs. ExPASy Swiss-Prot
Match: Q9LWM4 (CBL-interacting protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK5 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.3e-183
Identity = 319/443 (72.01%), Postives = 375/443 (84.65%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKK +ILM RYELGR+LGQGTFAKVYHARNL + QSVAIK+IDKEKVLRVG+IDQIKRE
Sbjct: 1   MEKKASILMNRYELGRMLGQGTFAKVYHARNLASNQSVAIKVIDKEKVLRVGMIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           IS+MRLVRHPN+VQL+EVMAS++KIYF MEYV+GGELF++VA+GRLKEDAARKYFQQLIG
Sbjct: 61  ISIMRLVRHPNIVQLHEVMASKSKIYFAMEYVRGGELFSRVARGRLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVD+CHSRGVYHRDLKPENLL+DE GNLK+SDFGLSA  E ++QDGLLHTTCGTPAYVAP
Sbjct: 121 AVDFCHSRGVYHRDLKPENLLVDENGNLKVSDFGLSAFKECQKQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           E+INK+GYDGAKADIWS GV+LFVLLAG+LPFHD NLMEMYR+IS+G+ K PQWF  +VR
Sbjct: 181 EIINKRGYDGAKADIWSCGVILFVLLAGYLPFHDSNLMEMYRKISKGDVKFPQWFTTDVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           +LLSR+LDP+P+ RI + KL+++ WFKKG+K  V   +P     + S+++ DVH AF   
Sbjct: 241 RLLSRLLDPNPNIRITVEKLVEHPWFKKGYKPAVMLSQP-----NESNNLKDVHTAF--- 300

Query: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA 360
            S +   N    +      +  S NAFDIISLS GFDLSGLFE+D +Q+  SRF T KPA
Sbjct: 301 -SADHKDNEGKAKEPASSLKPVSLNAFDIISLSKGFDLSGLFENDKEQKADSRFMTQKPA 360

Query: 361 SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA 420
           SAIVSK E+IAE E FKV K+DG+VKLQGSK+GRKG+LAIDAEIFEVTPSFFVVEVKK+A
Sbjct: 361 SAIVSKLEQIAETESFKVKKQDGLVKLQGSKEGRKGQLAIDAEIFEVTPSFFVVEVKKSA 420

Query: 421 GDTLEYRQFYNKDIRPCLKDIVW 444
           GDTLEY +F NK +RP L+DI W
Sbjct: 421 GDTLEYEKFCNKGLRPSLRDICW 434

[Close]

BLAST of MC03g_new0326 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 8.7e-177
Identity = 314/457 (68.71%), Postives = 374/457 (81.84%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           M+K G +LM++YELGRLLGQGTFAKVYHARN+ TG+SVAIK+IDK+KV +VGLIDQIKRE
Sbjct: 1   MDKNGIVLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHP+VV L+EVMAS+TKIYF MEYVKGGELF+KV+KG+LKE+ ARKYFQQLIG
Sbjct: 61  ISVMRLVRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           A+DYCHSRGVYHRDLKPENLLLDE G+LKISDFGLSAL ES++QDGLLHTTCGTPAYVAP
Sbjct: 121 AIDYCHSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVI KKGYDGAKAD+WS GVVL+VLLAGFLPFH+ NL+EMYR+I++GEFKCP WFPPEV+
Sbjct: 181 EVIGKKGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVK 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDP+P++RI + K+M+NSWF+KGFK  + TPK    +  + S +SDVHAAF   
Sbjct: 241 KLLSRILDPNPNSRIKIEKIMENSWFQKGFK-KIETPKSPE-SHQIDSLISDVHAAF--- 300

Query: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDII-SLSPGFDLSGLFESDVDQRLQSRFTTAKP 360
                        S KP     S+NAFD+I SLS GFDLSGLFE   ++R +S+FTT K 
Sbjct: 301 -------------SVKP----MSYNAFDLISSLSQGFDLSGLFEK--EERSESKFTTKKD 360

Query: 361 ASAIVSKFEEIA-EMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKK 420
           A  IVSKFEEIA   ERF + K D  VK++  ++GRKG LAID EIFEVT SF +VE KK
Sbjct: 361 AKEIVSKFEEIATSSERFNLTKSDVGVKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKK 420

Query: 421 TAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQ 456
           + GDT+EY+QF ++++RP LKDIVW W+GN      +
Sbjct: 421 SGGDTMEYKQFCDRELRPSLKDIVWKWQGNNNNSNNE 433

[Close]

BLAST of MC03g_new0326 vs. ExPASy Swiss-Prot
Match: Q5W736 (CBL-interacting protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK18 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.8e-172
Identity = 297/465 (63.87%), Postives = 374/465 (80.43%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           +EK G IL++RYE+G+LLGQGTFAKVYH RN+ T QSVAIK+IDK+K+ +VGL+DQIKRE
Sbjct: 4   LEKNGNILLRRYEIGKLLGQGTFAKVYHGRNIVTSQSVAIKVIDKDKIFKVGLMDQIKRE 63

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVM+LVRHPN+VQLYEVMA+++KIYFV+EYVKGGELFNKVAKGRLKEDAARKYFQQL+ 
Sbjct: 64  ISVMKLVRHPNIVQLYEVMATKSKIYFVLEYVKGGELFNKVAKGRLKEDAARKYFQQLVS 123

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVD+CHSRGVYHRDLKPENLL+DE GNLKI+DFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 124 AVDFCHSRGVYHRDLKPENLLVDENGNLKITDFGLSALAESRRQDGLLHTTCGTPAYVAP 183

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVI++KGYDG K D WS GV+LFVL+AG+LPF D NLMEMYR+I + EFKCP WF  +VR
Sbjct: 184 EVISRKGYDGVKVDTWSCGVILFVLMAGYLPFQDSNLMEMYRKIGKAEFKCPAWFSSDVR 243

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKA-----NVATPKPLRINGDVSSSVSDVHA 300
           KL+SRILDP+P +R+ + K+M+  WFKKG  +     NV T +P+       ++++DV+ 
Sbjct: 244 KLVSRILDPNPRSRMPITKIMETYWFKKGLDSKLILKNVETNEPV-------TALADVNV 303

Query: 301 AFDDISSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFT 360
            F  + S          ++GK     ++ NAFDIISLS GFDLSGLFE + D++ ++RFT
Sbjct: 304 VFSSMGSSSSKKTEEKQDAGK----LTNLNAFDIISLSEGFDLSGLFE-ETDKKKEARFT 363

Query: 361 TAKPASAIVSKFEEIAEMERFKVLKKD-GIVKLQGSKQGRKGELAIDAEIFEVTPSFFVV 420
           +++ ASAI+SK E++A   +  V KK+ G++K++G+ +GRKG LAIDAEIFEVTPSF +V
Sbjct: 364 SSQSASAIISKLEDVASCSKLTVKKKEGGVLKMEGASEGRKGVLAIDAEIFEVTPSFHLV 423

Query: 421 EVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQ 460
           E+KK  GDTLEY+  + +D++P LKDIVW W+G  Q QQ +   Q
Sbjct: 424 EIKKNNGDTLEYQHLWKEDMKPALKDIVWAWQGERQDQQPEDHGQ 456

[Close]

BLAST of MC03g_new0326 vs. ExPASy Swiss-Prot
Match: Q7X996 (CBL-interacting protein kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK2 PE=2 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.2e-170
Identity = 302/456 (66.23%), Postives = 369/456 (80.92%), Query Frame = 0


Query: 2   EKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKREI 61
           E++G +LM++YE+G+LLGQGTFAKVYHARN  T +SVAIK+IDKEKVL+ GL+DQIKREI
Sbjct: 3   EQRGNMLMKKYEMGKLLGQGTFAKVYHARNTETSESVAIKMIDKEKVLKGGLMDQIKREI 62

Query: 62  SVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIGA 121
           SVM+LVRHPN+VQLYEVMA++TKIYFV+E+VKGGELFNKV +GRLKEDAARKYFQQLI A
Sbjct: 63  SVMKLVRHPNIVQLYEVMATKTKIYFVLEHVKGGELFNKVQRGRLKEDAARKYFQQLICA 122

Query: 122 VDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPE 181
           VD+CHSRGVYHRDLKPENLLLDE  NLK+SDFGLSALA+ +RQDGLLHTTCGTPAYVAPE
Sbjct: 123 VDFCHSRGVYHRDLKPENLLLDENSNLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAPE 182

Query: 182 VINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVRK 241
           VIN++GYDGAKADIWS GV+LFVLLAG+LPFHD NLM+MY++I + EFKCP WF  +VR+
Sbjct: 183 VINRRGYDGAKADIWSCGVILFVLLAGYLPFHDKNLMDMYKKIGKAEFKCPSWFNTDVRR 242

Query: 242 LLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDIS 301
           LL RILDP+P TRI+M K+M+N WF+KG  A     K LR N     ++        D+S
Sbjct: 243 LLLRILDPNPSTRISMDKIMENPWFRKGLDA-----KLLRYNLQPKDAIP------VDMS 302

Query: 302 SEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPAS 361
           ++  S N       KP    S+ NAFDIISLS G DLSG+FE     + +S+FT+   AS
Sbjct: 303 TDFDSFNSAPTLEKKP----SNLNAFDIISLSTGLDLSGMFEE--SDKKESKFTSTSTAS 362

Query: 362 AIVSKFEEIAEMERFKVLKKD-GIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA 421
            I+SK E+IA+  R K+ KKD G++K++GSK GRKG + IDAEIFEVTP+F +VE+KKT 
Sbjct: 363 TIISKIEDIAKGLRLKLTKKDGGLLKMEGSKPGRKGVMGIDAEIFEVTPNFHLVELKKTN 422

Query: 422 GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQP 457
           GDTLEYR+  N+++RP LKDIVW W+G + +QQQQP
Sbjct: 423 GDTLEYRKVLNQEMRPALKDIVWAWQGEQPKQQQQP 441

[Close]

BLAST of MC03g_new0326 vs. ExPASy Swiss-Prot
Match: Q9C562 (CBL-interacting serine/threonine-protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN=CIPK10 PE=1 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 1.8e-158
Identity = 282/474 (59.49%), Postives = 363/474 (76.58%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           ME K ++L  +Y++GRLLGQGTFAKVY+ R++ T QSVAIK+IDKEKV++VGLI+QIKRE
Sbjct: 1   MENKPSVLTDKYDVGRLLGQGTFAKVYYGRSILTNQSVAIKMIDKEKVMKVGLIEQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMR+ RHPNVV+LYEVMA++T+IYFVMEY KGGELFNKVAKG+L++D A KYF QLI 
Sbjct: 61  ISVMRIARHPNVVELYEVMATKTRIYFVMEYCKGGELFNKVAKGKLRDDVAWKYFYQLIN 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVD+CHSR VYHRD+KPENLLLD+  NLK+SDFGLSALA+ +RQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDFCHSREVYHRDIKPENLLLDDNENLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVIN+KGYDG KADIWS GVVLFVLLAG+LPFHD NLMEMYR+I + +FK P WF PEVR
Sbjct: 181 EVINRKGYDGTKADIWSCGVVLFVLLAGYLPFHDSNLMEMYRKIGKADFKAPSWFAPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKG--FKANVATPKPLRING-DVSSSVSDVHAAF 300
           +LL ++LDP+P TRI +A++ ++SWF+KG   K      +   IN  +  ++ ++ + A 
Sbjct: 241 RLLCKMLDPNPETRITIARIRESSWFRKGLHMKQKKMEKRVKEINSVEAGTAGTNENGAG 300

Query: 301 DDISSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTA 360
              +    S NG+           ++ NAFD+I+LS GFDL+GLF    D + +SRFT+ 
Sbjct: 301 PSENGAGPSENGDRVTEENHTDEPTNLNAFDLIALSAGFDLAGLFGD--DNKRESRFTSQ 360

Query: 361 KPASAIVSKFEEIAEMERFKVLKKD-GIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEV 420
           KPAS I+SK EE+A+  +  + K++ G+ KL+  K+GRKG L++DAEIF+VTP+F +VEV
Sbjct: 361 KPASVIISKLEEVAQRLKLSIRKREAGLFKLERLKEGRKGILSMDAEIFQVTPNFHLVEV 420

Query: 421 KKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNE-----------QQQQQQPQPQ 460
           KK+ GDTLEY++   +D+RP L DIVW W+G +           QQQQQQ Q +
Sbjct: 421 KKSNGDTLEYQKLVAEDLRPALSDIVWVWQGEKDELTSQQETEYQQQQQQEQQE 472

[Close]

BLAST of MC03g_new0326 vs. NCBI nr
Match: XP_022139866.1 (CBL-interacting protein kinase 5 [Momordica charantia])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI
Sbjct: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300

Query: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA 360
           SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA
Sbjct: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA 360

Query: 361 SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA 420
           SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA
Sbjct: 361 SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA 420

Query: 421 GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461
           GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV
Sbjct: 421 GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461

[Close]

BLAST of MC03g_new0326 vs. NCBI nr
Match: XP_038893616.1 (CBL-interacting protein kinase 5 [Benincasa hispida])

HSP 1 Score: 834 bits (2154), Expect = 2.31e-303
Identity = 419/468 (89.53%), Postives = 443/468 (94.66%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKG ILMQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGTILMQRYELGRMLGQGTFAKVYHARNLRSGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMAS+TKIYF MEYVKGGELFNKV KG+LKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASKTKIYFAMEYVKGGELFNKVVKGKLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRD+KPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDIKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAG+LPFHDPNLME+YR+ISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGYLPFHDPNLMELYRKISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSPHTRINMAKLM+NSWFKKGFKANV TPK L+IN DVS  +S++H+AFDD+
Sbjct: 241 KLLSRILDPSPHTRINMAKLMENSWFKKGFKANVTTPKQLQINQDVSY-MSEIHSAFDDV 300

Query: 301 SSEEGSINGNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRF 360
           SSEEGS   N +ESGKPPT      R++SFNAFDIISLSPGFDLSGLFESDV++R Q+RF
Sbjct: 301 SSEEGSSISNTDESGKPPTPARTPMRHNSFNAFDIISLSPGFDLSGLFESDVNERPQTRF 360

Query: 361 TTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVV 420
           TT KPASAIVSKFEEIAEMERFKV+KKDGIVKLQGSKQGRKG+L IDAEIFEVTPSFFVV
Sbjct: 361 TTTKPASAIVSKFEEIAEMERFKVMKKDGIVKLQGSKQGRKGQLTIDAEIFEVTPSFFVV 420

Query: 421 EVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQ-PQPQIV 461
           EVKKTAGDTLEYRQFYNKD+RPCLKDIVWTWEGNEQQQQQQ PQ Q V
Sbjct: 421 EVKKTAGDTLEYRQFYNKDMRPCLKDIVWTWEGNEQQQQQQQPQSQTV 467

[Close]

BLAST of MC03g_new0326 vs. NCBI nr
Match: XP_008443358.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 20 [Cucumis melo])

HSP 1 Score: 833 bits (2153), Expect = 2.82e-303
Identity = 424/467 (90.79%), Postives = 442/467 (94.65%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKG+ILMQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGSILMQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYF MEYVKGGELFNKVAKG+LKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFAMEYVKGGELFNKVAKGKLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRD+KPENLLLDE+GNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDIKPENLLLDEYGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSPHTRINMAKLM+NSWFKKGFKANV TPK L+ N D+SS VSDVH+AF DI
Sbjct: 241 KLLSRILDPSPHTRINMAKLMENSWFKKGFKANVTTPKQLQNNEDMSS-VSDVHSAFLDI 300

Query: 301 SSEEGSINGNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRF 360
           SSE GS   N NESGKPPT      RN+SFNAFDIISLSPGFDLSGLFESDV +R QSRF
Sbjct: 301 SSEGGSSVSNTNESGKPPTATRAPIRNNSFNAFDIISLSPGFDLSGLFESDVSERPQSRF 360

Query: 361 TTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVV 420
           TT KPASAIVSKFEEIAEMERF+VLKKDGIVKLQGSKQGRKG+LAIDAEIFEVTPSFFVV
Sbjct: 361 TTKKPASAIVSKFEEIAEMERFQVLKKDGIVKLQGSKQGRKGQLAIDAEIFEVTPSFFVV 420

Query: 421 EVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461
           EVKKTAGDTLEYRQFY+KD+RPCLKDIVWTWEGNEQQQQQ   PQIV
Sbjct: 421 EVKKTAGDTLEYRQFYDKDMRPCLKDIVWTWEGNEQQQQQ---PQIV 463

[Close]

BLAST of MC03g_new0326 vs. NCBI nr
Match: KAA0057046.1 (CBL-interacting serine/threonine-protein kinase 20 [Cucumis melo var. makuwa])

HSP 1 Score: 820 bits (2118), Expect = 4.49e-298
Identity = 417/459 (90.85%), Postives = 434/459 (94.55%), Query Frame = 0


Query: 9   MQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVR 68
           MQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVR
Sbjct: 1   MQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVR 60

Query: 69  HPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIGAVDYCHSR 128
           HPNVVQLYEVMASRTKIYF MEYVKGGELFNKVAKG+LKEDAARKYFQQLIGAVDYCHSR
Sbjct: 61  HPNVVQLYEVMASRTKIYFAMEYVKGGELFNKVAKGKLKEDAARKYFQQLIGAVDYCHSR 120

Query: 129 GVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGY 188
           GVYHRD+KPENLLLDE+GNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGY
Sbjct: 121 GVYHRDIKPENLLLDEYGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGY 180

Query: 189 DGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVRKLLSRILD 248
           DGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCPQWFPPEVRKLLSRILD
Sbjct: 181 DGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPQWFPPEVRKLLSRILD 240

Query: 249 PSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDISSEEGSIN 308
           PSPHTRINMAKLM+NSWFKKGFKANV TPK L+ N D+SS VSDVH+AF DISSE GS  
Sbjct: 241 PSPHTRINMAKLMENSWFKKGFKANVTTPKQLQNNEDMSS-VSDVHSAFLDISSEGGSSV 300

Query: 309 GNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPASA 368
            N NESGKPPT      RN+SFNAFDIISLSPGFDLSGLFESDV +R QSRFTT KPASA
Sbjct: 301 SNTNESGKPPTATRAPIRNNSFNAFDIISLSPGFDLSGLFESDVSERPQSRFTTKKPASA 360

Query: 369 IVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTAGD 428
           IVSKFEEIAEMERF+VLKKDGIVKLQGSKQGRKG+LAIDAEIFEVTPSFFVVEVKKTAGD
Sbjct: 361 IVSKFEEIAEMERFQVLKKDGIVKLQGSKQGRKGQLAIDAEIFEVTPSFFVVEVKKTAGD 420

Query: 429 TLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461
           TLEYRQFY+KD+RPCLKDIVWTWEGNEQQQQQ   PQIV
Sbjct: 421 TLEYRQFYDKDMRPCLKDIVWTWEGNEQQQQQ---PQIV 455

[Close]

BLAST of MC03g_new0326 vs. NCBI nr
Match: XP_004150214.1 (CBL-interacting protein kinase 5 [Cucumis sativus] >KGN65626.1 hypothetical protein Csa_019692 [Cucumis sativus])

HSP 1 Score: 818 bits (2114), Expect = 2.47e-297
Identity = 412/460 (89.57%), Postives = 435/460 (94.57%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKG+ILMQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGSILMQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYF MEYV+GGELFNKVAKG+LKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFAMEYVRGGELFNKVAKGKLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRD+KPENLLLDE+GNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDIKPENLLLDEYGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSP TRINMAKLM+NSWFKKGFK NV TPK L+ + D+SS VSDV +AF DI
Sbjct: 241 KLLSRILDPSPRTRINMAKLMENSWFKKGFKENVTTPKQLQNDEDMSS-VSDVSSAFSDI 300

Query: 301 SSEEGSINGNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRF 360
           SSE GS   N NESG+PPT      RN+SFNAFD+ISLSPGFDLSGLFESDV++RLQSRF
Sbjct: 301 SSEGGSSVSNTNESGRPPTATRAPIRNNSFNAFDLISLSPGFDLSGLFESDVNERLQSRF 360

Query: 361 TTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVV 420
           TT KPASAIVSK EEIAEMERFKV+KKDGIVKLQGSKQGRKG+LAIDAEIFEVTPSF VV
Sbjct: 361 TTTKPASAIVSKLEEIAEMERFKVMKKDGIVKLQGSKQGRKGQLAIDAEIFEVTPSFSVV 420

Query: 421 EVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQ 454
           EVKKTAGDTLEYRQFY+KD+RPCLKDIVWTWEGNEQQQQQ
Sbjct: 421 EVKKTAGDTLEYRQFYDKDMRPCLKDIVWTWEGNEQQQQQ 459

[Close]

BLAST of MC03g_new0326 vs. ExPASy TrEMBL
Match: A0A6J1CDZ5 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111010675 PE=3 SV=1)

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI
Sbjct: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300

Query: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA 360
           SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA
Sbjct: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA 360

Query: 361 SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA 420
           SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA
Sbjct: 361 SAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTA 420

Query: 421 GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461
           GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV
Sbjct: 421 GDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461

[Close]

BLAST of MC03g_new0326 vs. ExPASy TrEMBL
Match: A0A1S3B8L8 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103486963 PE=3 SV=1)

HSP 1 Score: 833 bits (2153), Expect = 1.37e-303
Identity = 424/467 (90.79%), Postives = 442/467 (94.65%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKG+ILMQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGSILMQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYF MEYVKGGELFNKVAKG+LKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFAMEYVKGGELFNKVAKGKLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRD+KPENLLLDE+GNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDIKPENLLLDEYGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSPHTRINMAKLM+NSWFKKGFKANV TPK L+ N D+SS VSDVH+AF DI
Sbjct: 241 KLLSRILDPSPHTRINMAKLMENSWFKKGFKANVTTPKQLQNNEDMSS-VSDVHSAFLDI 300

Query: 301 SSEEGSINGNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRF 360
           SSE GS   N NESGKPPT      RN+SFNAFDIISLSPGFDLSGLFESDV +R QSRF
Sbjct: 301 SSEGGSSVSNTNESGKPPTATRAPIRNNSFNAFDIISLSPGFDLSGLFESDVSERPQSRF 360

Query: 361 TTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVV 420
           TT KPASAIVSKFEEIAEMERF+VLKKDGIVKLQGSKQGRKG+LAIDAEIFEVTPSFFVV
Sbjct: 361 TTKKPASAIVSKFEEIAEMERFQVLKKDGIVKLQGSKQGRKGQLAIDAEIFEVTPSFFVV 420

Query: 421 EVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461
           EVKKTAGDTLEYRQFY+KD+RPCLKDIVWTWEGNEQQQQQ   PQIV
Sbjct: 421 EVKKTAGDTLEYRQFYDKDMRPCLKDIVWTWEGNEQQQQQ---PQIV 463

[Close]

BLAST of MC03g_new0326 vs. ExPASy TrEMBL
Match: A0A5A7USA3 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002340 PE=3 SV=1)

HSP 1 Score: 820 bits (2118), Expect = 2.17e-298
Identity = 417/459 (90.85%), Postives = 434/459 (94.55%), Query Frame = 0


Query: 9   MQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVR 68
           MQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVR
Sbjct: 1   MQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKIIDKEKVLRVGLIDQIKREISVMRLVR 60

Query: 69  HPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIGAVDYCHSR 128
           HPNVVQLYEVMASRTKIYF MEYVKGGELFNKVAKG+LKEDAARKYFQQLIGAVDYCHSR
Sbjct: 61  HPNVVQLYEVMASRTKIYFAMEYVKGGELFNKVAKGKLKEDAARKYFQQLIGAVDYCHSR 120

Query: 129 GVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGY 188
           GVYHRD+KPENLLLDE+GNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGY
Sbjct: 121 GVYHRDIKPENLLLDEYGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKKGY 180

Query: 189 DGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVRKLLSRILD 248
           DGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCPQWFPPEVRKLLSRILD
Sbjct: 181 DGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPQWFPPEVRKLLSRILD 240

Query: 249 PSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDISSEEGSIN 308
           PSPHTRINMAKLM+NSWFKKGFKANV TPK L+ N D+SS VSDVH+AF DISSE GS  
Sbjct: 241 PSPHTRINMAKLMENSWFKKGFKANVTTPKQLQNNEDMSS-VSDVHSAFLDISSEGGSSV 300

Query: 309 GNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPASA 368
            N NESGKPPT      RN+SFNAFDIISLSPGFDLSGLFESDV +R QSRFTT KPASA
Sbjct: 301 SNTNESGKPPTATRAPIRNNSFNAFDIISLSPGFDLSGLFESDVSERPQSRFTTKKPASA 360

Query: 369 IVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTAGD 428
           IVSKFEEIAEMERF+VLKKDGIVKLQGSKQGRKG+LAIDAEIFEVTPSFFVVEVKKTAGD
Sbjct: 361 IVSKFEEIAEMERFQVLKKDGIVKLQGSKQGRKGQLAIDAEIFEVTPSFFVVEVKKTAGD 420

Query: 429 TLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQIV 461
           TLEYRQFY+KD+RPCLKDIVWTWEGNEQQQQQ   PQIV
Sbjct: 421 TLEYRQFYDKDMRPCLKDIVWTWEGNEQQQQQ---PQIV 455

[Close]

BLAST of MC03g_new0326 vs. ExPASy TrEMBL
Match: A0A0A0M090 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G470420 PE=3 SV=1)

HSP 1 Score: 818 bits (2114), Expect = 1.20e-297
Identity = 412/460 (89.57%), Postives = 435/460 (94.57%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKKG+ILMQRYELGR+LGQGTFAKVYHARNL +GQSVAIKIIDKEKVLRVGLIDQIKRE
Sbjct: 1   MEKKGSILMQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKIIDKEKVLRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYF MEYV+GGELFNKVAKG+LKEDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFAMEYVRGGELFNKVAKGKLKEDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRD+KPENLLLDE+GNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDIKPENLLLDEYGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCPQWFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPQWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDPSP TRINMAKLM+NSWFKKGFK NV TPK L+ + D+SS VSDV +AF DI
Sbjct: 241 KLLSRILDPSPRTRINMAKLMENSWFKKGFKENVTTPKQLQNDEDMSS-VSDVSSAFSDI 300

Query: 301 SSEEGSINGNNNESGKPPT------RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRF 360
           SSE GS   N NESG+PPT      RN+SFNAFD+ISLSPGFDLSGLFESDV++RLQSRF
Sbjct: 301 SSEGGSSVSNTNESGRPPTATRAPIRNNSFNAFDLISLSPGFDLSGLFESDVNERLQSRF 360

Query: 361 TTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVV 420
           TT KPASAIVSK EEIAEMERFKV+KKDGIVKLQGSKQGRKG+LAIDAEIFEVTPSF VV
Sbjct: 361 TTTKPASAIVSKLEEIAEMERFKVMKKDGIVKLQGSKQGRKGQLAIDAEIFEVTPSFSVV 420

Query: 421 EVKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQ 454
           EVKKTAGDTLEYRQFY+KD+RPCLKDIVWTWEGNEQQQQQ
Sbjct: 421 EVKKTAGDTLEYRQFYDKDMRPCLKDIVWTWEGNEQQQQQ 459

[Close]

BLAST of MC03g_new0326 vs. ExPASy TrEMBL
Match: A0A6J1EHA2 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111434174 PE=3 SV=1)

HSP 1 Score: 802 bits (2071), Expect = 3.26e-291
Identity = 400/455 (87.91%), Postives = 429/455 (94.29%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           MEKK +ILMQRYELGR+LGQGTFAKVYHARNL +GQSVAIK+I+KEKV+RVGLIDQIKRE
Sbjct: 1   MEKKSSILMQRYELGRMLGQGTFAKVYHARNLKSGQSVAIKVINKEKVMRVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHPNVVQLYEVMASRTKIYF MEYVKGGELF+KVA+G+L+EDAARKYFQQLIG
Sbjct: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFAMEYVKGGELFDKVAEGKLREDAARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVDYCHSRGVYHRD+KPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDYCHSRGVYHRDIKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVINKKGYDGAKADIWSSGVVLFVLLAG+LPFHDPNLMEMYR+ISRGEFKCP WFPPEVR
Sbjct: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGYLPFHDPNLMEMYRKISRGEFKCPHWFPPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDP+P TRI MAKLM+NSWFKKGF ANVATPKPL+ING++S  VSDVH  FDD 
Sbjct: 241 KLLSRILDPNPQTRIKMAKLMENSWFKKGFSANVATPKPLQINGNMSG-VSDVHIDFDDS 300

Query: 301 SSEEGSINGNNNESGKPPT-----RNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFT 360
           SSEEGS++  +NESG PP      RN SFNAFDIISLSPGFDLSGLFESDV++R QSRFT
Sbjct: 301 SSEEGSVS-TSNESGTPPAGRTPRRNHSFNAFDIISLSPGFDLSGLFESDVNERPQSRFT 360

Query: 361 TAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVE 420
           T KP SAIVSKFEEIAEMERFKV+KKDG+VKLQGSKQGRKG+LAIDAEIFEVTPSFFVVE
Sbjct: 361 TTKPESAIVSKFEEIAEMERFKVMKKDGLVKLQGSKQGRKGQLAIDAEIFEVTPSFFVVE 420

Query: 421 VKKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQ 450
           V KTAGDTLEYR+FY+KD+RPCLKDIVWTWEGNEQ
Sbjct: 421 VTKTAGDTLEYRKFYDKDMRPCLKDIVWTWEGNEQ 453

[Close]

BLAST of MC03g_new0326 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 621.3 bits (1601), Expect = 6.2e-178
Identity = 314/457 (68.71%), Postives = 374/457 (81.84%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           M+K G +LM++YELGRLLGQGTFAKVYHARN+ TG+SVAIK+IDK+KV +VGLIDQIKRE
Sbjct: 1   MDKNGIVLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMRLVRHP+VV L+EVMAS+TKIYF MEYVKGGELF+KV+KG+LKE+ ARKYFQQLIG
Sbjct: 61  ISVMRLVRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVSKGKLKENIARKYFQQLIG 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           A+DYCHSRGVYHRDLKPENLLLDE G+LKISDFGLSAL ES++QDGLLHTTCGTPAYVAP
Sbjct: 121 AIDYCHSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVI KKGYDGAKAD+WS GVVL+VLLAGFLPFH+ NL+EMYR+I++GEFKCP WFPPEV+
Sbjct: 181 EVIGKKGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVK 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           KLLSRILDP+P++RI + K+M+NSWF+KGFK  + TPK    +  + S +SDVHAAF   
Sbjct: 241 KLLSRILDPNPNSRIKIEKIMENSWFQKGFK-KIETPKSPE-SHQIDSLISDVHAAF--- 300

Query: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDII-SLSPGFDLSGLFESDVDQRLQSRFTTAKP 360
                        S KP     S+NAFD+I SLS GFDLSGLFE   ++R +S+FTT K 
Sbjct: 301 -------------SVKP----MSYNAFDLISSLSQGFDLSGLFEK--EERSESKFTTKKD 360

Query: 361 ASAIVSKFEEIA-EMERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKK 420
           A  IVSKFEEIA   ERF + K D  VK++  ++GRKG LAID EIFEVT SF +VE KK
Sbjct: 361 AKEIVSKFEEIATSSERFNLTKSDVGVKMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKK 420

Query: 421 TAGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQ 456
           + GDT+EY+QF ++++RP LKDIVW W+GN      +
Sbjct: 421 SGGDTMEYKQFCDRELRPSLKDIVWKWQGNNNNSNNE 433

[Close]

BLAST of MC03g_new0326 vs. TAIR 10
Match: AT5G58380.1 (SOS3-interacting protein 1 )

HSP 1 Score: 560.5 bits (1443), Expect = 1.3e-159
Identity = 282/474 (59.49%), Postives = 363/474 (76.58%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           ME K ++L  +Y++GRLLGQGTFAKVY+ R++ T QSVAIK+IDKEKV++VGLI+QIKRE
Sbjct: 1   MENKPSVLTDKYDVGRLLGQGTFAKVYYGRSILTNQSVAIKMIDKEKVMKVGLIEQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMR+ RHPNVV+LYEVMA++T+IYFVMEY KGGELFNKVAKG+L++D A KYF QLI 
Sbjct: 61  ISVMRIARHPNVVELYEVMATKTRIYFVMEYCKGGELFNKVAKGKLRDDVAWKYFYQLIN 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVD+CHSR VYHRD+KPENLLLD+  NLK+SDFGLSALA+ +RQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDFCHSREVYHRDIKPENLLLDDNENLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVIN+KGYDG KADIWS GVVLFVLLAG+LPFHD NLMEMYR+I + +FK P WF PEVR
Sbjct: 181 EVINRKGYDGTKADIWSCGVVLFVLLAGYLPFHDSNLMEMYRKIGKADFKAPSWFAPEVR 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKG--FKANVATPKPLRING-DVSSSVSDVHAAF 300
           +LL ++LDP+P TRI +A++ ++SWF+KG   K      +   IN  +  ++ ++ + A 
Sbjct: 241 RLLCKMLDPNPETRITIARIRESSWFRKGLHMKQKKMEKRVKEINSVEAGTAGTNENGAG 300

Query: 301 DDISSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTA 360
              +    S NG+           ++ NAFD+I+LS GFDL+GLF    D + +SRFT+ 
Sbjct: 301 PSENGAGPSENGDRVTEENHTDEPTNLNAFDLIALSAGFDLAGLFGD--DNKRESRFTSQ 360

Query: 361 KPASAIVSKFEEIAEMERFKVLKKD-GIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEV 420
           KPAS I+SK EE+A+  +  + K++ G+ KL+  K+GRKG L++DAEIF+VTP+F +VEV
Sbjct: 361 KPASVIISKLEEVAQRLKLSIRKREAGLFKLERLKEGRKGILSMDAEIFQVTPNFHLVEV 420

Query: 421 KKTAGDTLEYRQFYNKDIRPCLKDIVWTWEGNE-----------QQQQQQPQPQ 460
           KK+ GDTLEY++   +D+RP L DIVW W+G +           QQQQQQ Q +
Sbjct: 421 KKSNGDTLEYQKLVAEDLRPALSDIVWVWQGEKDELTSQQETEYQQQQQQEQQE 472

[Close]

BLAST of MC03g_new0326 vs. TAIR 10
Match: AT5G07070.1 (CBL-interacting protein kinase 2 )

HSP 1 Score: 555.8 bits (1431), Expect = 3.2e-158
Identity = 276/460 (60.00%), Postives = 356/460 (77.39%), Query Frame = 0


Query: 1   MEKKGAILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKRE 60
           ME K ++L +RYE+GRLLGQGTFAKVY  R+ HT +SVAIK+IDK+KV+RVGL  QIKRE
Sbjct: 1   MENKPSVLTERYEVGRLLGQGTFAKVYFGRSNHTNESVAIKMIDKDKVMRVGLSQQIKRE 60

Query: 61  ISVMRLVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIG 120
           ISVMR+ +HPNVV+LYEVMA++++IYFV+EY KGGELFNKVAKG+LKED A KYF QLI 
Sbjct: 61  ISVMRIAKHPNVVELYEVMATKSRIYFVIEYCKGGELFNKVAKGKLKEDVAWKYFYQLIS 120

Query: 121 AVDYCHSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAP 180
           AVD+CHSRGVYHRD+KPENLLLD+  NLK+SDFGLSALA+ +RQDGLLHTTCGTPAYVAP
Sbjct: 121 AVDFCHSRGVYHRDIKPENLLLDDNDNLKVSDFGLSALADCKRQDGLLHTTCGTPAYVAP 180

Query: 181 EVINKKGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVR 240
           EVIN+KGY+G KADIWS GVVLFVLLAG+LPFHD NLMEMYR+I + +FKCP WF PEV+
Sbjct: 181 EVINRKGYEGTKADIWSCGVVLFVLLAGYLPFHDTNLMEMYRKIGKADFKCPSWFAPEVK 240

Query: 241 KLLSRILDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDI 300
           +LL ++LDP+  TRI +AK+ ++SWF+KG        + +     V  + + + A     
Sbjct: 241 RLLCKMLDPNHETRITIAKIKESSWFRKGLHLKQKKMEKME-KQQVREATNPMEAG---- 300

Query: 301 SSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSRFTTAKPA 360
               GS    N E+ +PP R ++ NAFDII+LS GF L+GLF  DV  + +SRF + KPA
Sbjct: 301 ----GSGQNENGENHEPP-RLATLNAFDIIALSTGFGLAGLF-GDVYDKRESRFASQKPA 360

Query: 361 SAIVSKFEEIAEMERFKVLKKD-GIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKT 420
           S I+SK  E+A+  + K+ K+  G+ KL+  K+G+ G L +DAEIF+VTP+F +VEVKK 
Sbjct: 361 SEIISKLVEVAKCLKLKIRKQGAGLFKLERVKEGKNGILTMDAEIFQVTPTFHLVEVKKC 420

Query: 421 AGDTLEYRQFYNKDIRPCLKDIVWTWEGNEQQQQQQPQPQ 460
            GDT+EY++   +D+RP L DIVW W+G +++++Q  Q +
Sbjct: 421 NGDTMEYQKLVEEDLRPALADIVWVWQGEKEKEEQLLQDE 449

[Close]

BLAST of MC03g_new0326 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 526.2 bits (1354), Expect = 2.7e-149
Identity = 262/440 (59.55%), Postives = 334/440 (75.91%), Query Frame = 0


Query: 7   ILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKREISVMRL 66
           +L  RYELGRLLG GTFAKVYHARN+ TG+SVA+K++ KEKV++VG++DQIKREISVMR+
Sbjct: 19  LLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRM 78

Query: 67  VRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIGAVDYCH 126
           V+HPN+V+L+EVMAS++KIYF ME V+GGELF KVAKGRL+ED AR YFQQLI AVD+CH
Sbjct: 79  VKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCH 138

Query: 127 SRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINKK 186
           SRGVYHRDLKPENLLLDE GNLK++DFGLSA  E  +QDGLLHTTCGTPAYVAPEVI KK
Sbjct: 139 SRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKK 198

Query: 187 GYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVRKLLSRI 246
           GYDGAKAD+WS GV+LFVLLAG+LPF D NL+ MYR+I RG+FKCP W   + R+L++++
Sbjct: 199 GYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKL 258

Query: 247 LDPSPHTRINMAKLMDNSWFKKGFKANVATPKPLRINGDVSSSVSDVHAAFDDISSEEGS 306
           LDP+P+TRI + K+MD+ WFKK  +A  +  +P      V+++++      D +      
Sbjct: 259 LDPNPNTRITIEKVMDSPWFKK--QATRSRNEP------VAATITTTEEDVDFLVH---- 318

Query: 307 INGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLF-ESDVDQRLQSRFTTAKPASAIVS 366
                    K      + NAF II+LS GFDLS LF E   +++ + RF T++PAS+++S
Sbjct: 319 ---------KSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVIS 378

Query: 367 KFEEIAEM-ERFKVLKKDGIVKLQGSKQGRKGELAIDAEIFEVTPSFFVVEVKKTAGDTL 426
             EE A +  +F V K +  V+++G + GRKG+LA++AEIF V PSF VVEVKK  GDTL
Sbjct: 379 SLEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTL 437

Query: 427 EYRQFYNKDIRPCLKDIVWT 445
           EY  F +  +RP LKDI WT
Sbjct: 439 EYNNFCSTALRPALKDIFWT 437

[Close]

BLAST of MC03g_new0326 vs. TAIR 10
Match: AT5G45810.1 (CBL-interacting protein kinase 19 )

HSP 1 Score: 525.8 bits (1353), Expect = 3.5e-149
Identity = 268/449 (59.69%), Postives = 331/449 (73.72%), Query Frame = 0


Query: 6   AILMQRYELGRLLGQGTFAKVYHARNLHTGQSVAIKIIDKEKVLRVGLIDQIKREISVMR 65
           A+++ +YE+GRLLG GTFAKVY ARN  +G+SVAIK+IDKEKVL+ GLI  IKREIS++R
Sbjct: 22  ALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIAHIKREISILR 81

Query: 66  LVRHPNVVQLYEVMASRTKIYFVMEYVKGGELFNKVAKGRLKEDAARKYFQQLIGAVDYC 125
            VRHPN+VQL+EVMA+++KIYFVMEYVKGGELFNKVAKGRLKE+ ARKYFQQLI AV +C
Sbjct: 82  RVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYFQQLISAVSFC 141

Query: 126 HSRGVYHRDLKPENLLLDEFGNLKISDFGLSALAESRRQDGLLHTTCGTPAYVAPEVINK 185
           H RGVYHRDLKPENLLLDE GNLK+SDFGLSA+++  RQDGL HT CGTPAYVAPEV+ +
Sbjct: 142 HFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLAR 201

Query: 186 KGYDGAKADIWSSGVVLFVLLAGFLPFHDPNLMEMYRRISRGEFKCPQWFPPEVRKLLSR 245
           KGYDGAK DIWS GV+LFVL+AGFLPFHD N+M MY++I RG+F+CP+WFP E+ +LL R
Sbjct: 202 KGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWFPVEINRLLIR 261

Query: 246 ILDPSPHTRINMAKLMDNSWFKKGFK------------ANVATPKPLRINGDVSSSVSDV 305
           +L+  P  R  M  +M+ SWFKKGFK             NVA       + D   S+  V
Sbjct: 262 MLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVA-------DDDEIESIESV 321

Query: 306 HAAFDDISSEEGSINGNNNESGKPPTRNSSFNAFDIISLSPGFDLSGLFESDVDQRLQSR 365
                 +S  E   + +    G    R +S NAFD+IS SPGFDLSGLFE D +    SR
Sbjct: 322 SGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGE---GSR 381

Query: 366 FTTAKPASAIVSKFEEIAEMERFKVLKKDGIVKLQGSKQG-RKGELAIDAEIFEVTPSFF 425
           F +  P   I+SK EEIA +  F V KKD  V L+GS++G  KG L+I AEIFE+TP+  
Sbjct: 382 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 441

Query: 426 VVEVKKTAGDTLEYRQFYNKDIRPCLKDI 442
           VVEVKK  GD +EY +F NK+++P L+++
Sbjct: 442 VVEVKKKGGDKMEYDEFCNKELKPKLQNL 460

The following BLAST results are available for this feature:

BLAST of MC03g_new0326 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LWM4	1.3e-183	72.01	CBL-interacting protein kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP...	[more]
Q9FJ54	8.7e-177	68.71	CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37...	[more]
Q5W736	3.8e-172	63.87	CBL-interacting protein kinase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=CI...	[more]
Q7X996	1.2e-170	66.23	CBL-interacting protein kinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP...	[more]
Q9C562	1.8e-158	59.49	CBL-interacting serine/threonine-protein kinase 10 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of MC03g_new0326 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139866.1	0.0	100.00	CBL-interacting protein kinase 5 [Momordica charantia]	[more]
XP_038893616.1	2.31e-303	89.53	CBL-interacting protein kinase 5 [Benincasa hispida]	[more]
XP_008443358.1	2.82e-303	90.79	PREDICTED: CBL-interacting serine/threonine-protein kinase 20 [Cucumis melo]	[more]
KAA0057046.1	4.49e-298	90.85	CBL-interacting serine/threonine-protein kinase 20 [Cucumis melo var. makuwa]	[more]
XP_004150214.1	2.47e-297	89.57	CBL-interacting protein kinase 5 [Cucumis sativus] >KGN65626.1 hypothetical prot...	[more]

BLAST of MC03g_new0326 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDZ5	0.0	100.00	Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L...	[more]
A0A1S3B8L8	1.37e-303	90.79	Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A5A7USA3	2.17e-298	90.85	Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A0A0M090	1.20e-297	89.57	Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1...	[more]
A0A6J1EHA2	3.26e-291	87.91	Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of MC03g_new0326 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45820.1	6.2e-178	68.71	CBL-interacting protein kinase 20	[more]
AT5G58380.1	1.3e-159	59.49	SOS3-interacting protein 1	[more]
AT5G07070.1	3.2e-158	60.00	CBL-interacting protein kinase 2	[more]
AT4G30960.1	2.7e-149	59.55	SOS3-interacting protein 3	[more]
AT5G45810.1	3.5e-149	59.69	CBL-interacting protein kinase 19	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 12..266 e-value: 1.4E-102 score: 356.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 12..266 e-value: 1.1E-72 score: 244.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 12..266 score: 52.738914
IPR004041	NAF domain	PFAM	PF03822	NAF	coord: 322..380 e-value: 8.6E-22 score: 77.1
None	No IPR available	GENE3D	3.30.310.80	Kinase associated domain 1, KA1	coord: 319..444 e-value: 1.7E-45 score: 156.1
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 3..314 e-value: 1.2E-103 score: 348.8
None	No IPR available	PIRSR	PIRSR000556-2	PIRSR000556-2	coord: 7..262 e-value: 4.4E-29 score: 99.3
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 11..261 e-value: 4.5E-12 score: 43.4
None	No IPR available	PIRSR	PIRSR037014-2	PIRSR037014-2	coord: 10..268 e-value: 1.7E-74 score: 249.4
None	No IPR available	PIRSR	PIRSR037921-2	PIRSR037921-2	coord: 16..227 e-value: 1.8E-12 score: 45.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 303..321
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 301..321
None	No IPR available	PANTHER	PTHR43895	FAMILY NOT NAMED	coord: 2..455
None	No IPR available	PANTHER	PTHR43895:SF3	CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 20	coord: 2..455
None	No IPR available	CDD	cd12195	CIPK_C	coord: 325..439 e-value: 3.66631E-54 score: 175.068
None	No IPR available	CDD	cd14663	STKc_SnRK3	coord: 11..265 e-value: 1.38259E-172 score: 482.675
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 18..41
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 130..142
IPR018451	NAF/FISL domain	PROSITE	PS50816	NAF	coord: 319..343 score: 13.270258
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 8..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0326.1	MC03g_new0326.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0007165	signal transduction
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0106310	protein serine kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004712	protein serine/threonine/tyrosine kinase activity
molecular_function	GO:0004672	protein kinase activity