MC03g_new0296 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g_new0296 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 6.8e-138
Identity = 248/513 (48.34%), Postives = 345/513 (67.25%), Query Frame = 0

Query: 7   SLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTP--RDPLYSSVLNFTI 66
           +L  + ++L    L+S+  ++ F+ C  + + + S  I+   ++P     L+   L  T 
Sbjct: 11  TLFLVTLLLISVPLSSSTLQQDFVKCL-VDNSDVSFPITASFFSPDQNATLFKEELESTA 70

Query: 67  QNLRFLDDSNPQ--IIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLS--DAP 126
           QNLR+L  SNP+   I  P   +H+QA+++CAK     +R+RSGGHDYEGLS+++  + P
Sbjct: 71  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 130

Query: 127 FLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFS 186
           F+IVDL  L+ +DVD++ N AW  +GATIGE+YYRI EKS+T GFPAGL S++G+GGH  
Sbjct: 131 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 190

Query: 187 GGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKI 246
           GG YG ++RKFG+ ADNV+DA  +DANG++ DR +MGEDVFWAIRGGGGGSFG++LAWKI
Sbjct: 191 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 250

Query: 247 KLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGI 306
           KLVPVP  VT+F +TK LEQ+ T +++KW+ +A K D+ L +R+++     + +   +  
Sbjct: 251 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNR-- 310

Query: 307 VLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLD-QPNTLL 366
            ++ +++A FLG    LL +MQK+FPEL +TK+DC EMSWI+SV+Y A  P    P  LL
Sbjct: 311 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL 370

Query: 367 DRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEI 426
                 K  +K KSD+V+EPI   GLEG+WE   EE       +  PYGG M++ISESEI
Sbjct: 371 AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWN--PYGGMMSRISESEI 430

Query: 427 AFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVG 486
            FPHR G L+KI +  +W  G+   +RH+ WI + Y Y+  +VSKNPR  YVNYRDLD+G
Sbjct: 431 PFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLG 490

Query: 487 TNNKNGYTSYEQASIWGVRYFKGNFKRLVSEGG 513
           TN   G T    A  WG +Y+KGNF+RLV   G
Sbjct: 491 TN--EGET---DAREWGAKYYKGNFERLVKIKG 513

BLAST of MC03g_new0296 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.4e-137
Identity = 247/507 (48.72%), Postives = 341/507 (67.26%), Query Frame = 0

Query: 11  IFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRD--PLYSSVLNFTIQNLR 70
           ++ +   +SLAS   ++ FI+C    + +    +    + P     ++S VL  T QNLR
Sbjct: 19  LYTITPTSSLASL--QDQFINCVQ-RNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLR 78

Query: 71  FLDDSNPQ--IIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLS--DAPFLIV 130
           FL  S P+   I +P   SH+QASIIC+K     +R+RSGGHDYEGLSY+S  D PF+++
Sbjct: 79  FLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILM 138

Query: 131 DLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFSGGGY 190
           DL  ++ ++++++ N AWVQSGAT+GELYYRIAEKSK  GFPAGL S++G+GGH +GG Y
Sbjct: 139 DLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAY 198

Query: 191 GLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKIKLVP 250
           G ++RK+G+ ADNV+DA  +DANGKL DR +MGED FWAIRGG GGSFGI+LAWKIKLVP
Sbjct: 199 GSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVP 258

Query: 251 VPPVVTIFKITKNLEQN-ATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGIVLT 310
           VP  VT+F +TK L+Q+    ++ KWQ VA K  E L +R+L +   +   +      +T
Sbjct: 259 VPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK-----TVT 318

Query: 311 ATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPNTLLDRGF 370
            ++ A FLG    L+++M+K+FPEL +T +DCIEMSW+ES+ Y +  P   P  +L +G 
Sbjct: 319 TSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGK 378

Query: 371 A--LKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEIAF 430
           +   K S+K KSD+V+ PI ++GL+GI++ L +E +     +  PYGG M KI ES+I F
Sbjct: 379 SPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWN--PYGGMMAKIPESQIPF 438

Query: 431 PHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVGTN 490
           PHR G L+K+ Y  SW   +    RHI+WI   Y Y+TP+VS NPR  YVNYRDLD+G N
Sbjct: 439 PHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRN 498

Query: 491 NKNGYTSYEQASIWGVRYFKGNFKRLV 509
            K+  T  +QA +WG  YFK NF RL+
Sbjct: 499 TKDVKTCIKQAQVWGANYFKNNFNRLM 515

BLAST of MC03g_new0296 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 1.4e-135
Identity = 245/507 (48.32%), Postives = 331/507 (65.29%), Query Frame = 0

Query: 11  IFVVLSFASLASAFN-REGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVLNFTIQNLRF 70
           IF  LSF    S  N RE F+ CFS H PN+  +   ++YT  D LY S+LN TIQNLRF
Sbjct: 15  IFFFLSFHIQISIANPRENFLKCFSKHIPNNVAN-PKLVYTQHDQLYMSILNSTIQNLRF 74

Query: 71  LDDSNPQ--IIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSDAPFLIVDLI 130
           + D+ P+  +IVTP   SHIQA+I+C+K  G Q+R RSGGHD EG+SY+S  PF++VDL 
Sbjct: 75  ISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLR 134

Query: 131 NLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFSGGGYGLL 190
           N+ SI +DV    AWV++GAT+GE+YY I EK++ L FP G   TVGVGGHFSGGGYG L
Sbjct: 135 NMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGAL 194

Query: 191 IRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKIKLVPVPP 250
           +R +G+AADN+IDA+ ++ +GK+ DRKSMGED+FWAIRGGGG +FGI+ AWKIKLV VP 
Sbjct: 195 MRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPS 254

Query: 251 VVTIFKITKNLE-QNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGIVLTATF 310
             TIF + KN+E      L +KWQ +A K D+ L+L       + ++   +    +   F
Sbjct: 255 KSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYF 314

Query: 311 KAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFA-----KLPLDQPNTLLDR 370
            + F G  + L+ +M K+FPEL + K DC E SWI++ ++++          +   LLDR
Sbjct: 315 SSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDR 374

Query: 371 GFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEIAF 430
               K ++  K DYV++PI +  +  I E L EE +      + PYGG M +ISES I F
Sbjct: 375 SAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPF 434

Query: 431 PHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVGTN 490
           PHRAG +Y++ YT SW   ED  ++HI+W+   Y++ TP+VS+NPR  Y+NYRDLD+G  
Sbjct: 435 PHRAGIMYELWYTASWEKQED-NEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKT 494

Query: 491 NKNGYTSYEQASIWGVRYFKGNFKRLV 509
           N     +Y QA IWG +YF  NF RLV
Sbjct: 495 NHASPNNYTQARIWGEKYFGKNFNRLV 519

BLAST of MC03g_new0296 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 2.4e-135
Identity = 245/511 (47.95%), Postives = 339/511 (66.34%), Query Frame = 0

Query: 4   TITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNII-YTPRDPLYSSVLNF 63
           T+  L+  ++ LSF+++     ++ F+ C   +S  S    S+   +      +   L  
Sbjct: 15  TLLLLSLSYIPLSFSTI-----QQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALET 74

Query: 64  TIQNLRFLDDSN--PQIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLS--D 123
           + QNLR+L  SN  P+ I  P   +H+QA+++CAK     +R+RSGGHDYEGLSY+S  +
Sbjct: 75  SAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEME 134

Query: 124 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 183
             F+IVDL  L+ I VD+E N AWV +GA+IGE+YYRI EKSK  GFPAGL +++G+GGH
Sbjct: 135 TAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGH 194

Query: 184 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 243
             GG YG ++RKFG+ ADNV+DA  +DA+GK+ +R +MGEDVFWAIRGGGGGSFG++LAW
Sbjct: 195 IIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAW 254

Query: 244 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 303
           KIKLVPVP +VT+F +T+ LEQ+ T L++KWQ VA K DE L +R+++     + +  E+
Sbjct: 255 KIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKER 314

Query: 304 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPN-T 363
              ++ +++  FLG    LL +MQ++FP+L +TK+DC+E SWI+SV+Y A  P   P+  
Sbjct: 315 --TISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEA 374

Query: 364 LLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISES 423
           LLD     K  +K KSDYVEEPI   GLEG+WE L EE       +  PYGG M KI E+
Sbjct: 375 LLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN--PYGGMMAKIPET 434

Query: 424 EIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLD 483
           E  FPHR+G L+KI +   W  G+    +H+ W+ + Y Y+  +VSK+PR+ YVNYRDLD
Sbjct: 435 ETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLD 494

Query: 484 VGTNNKNGYTSYEQASIWGVRYFKGNFKRLV 509
           +G N K        A  WG RYFKGNF+RLV
Sbjct: 495 LGMNGKG-----SDAREWGNRYFKGNFERLV 511

BLAST of MC03g_new0296 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.7e-134
Identity = 236/490 (48.16%), Postives = 334/490 (68.16%), Query Frame = 0

Query: 21  ASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVLNFTIQNLRFL--DDSNPQII 80
           A+  N+  F+ C SL   N S+ +S +I+TP D  +SSVL  +IQN RF   D   P +I
Sbjct: 29  ANRSNQSSFLQCLSLQL-NDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 88

Query: 81  VTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSDAPFLIVDLINLQSIDVDVEK 140
           +TP Q S +Q+++ CA+  G  +R RSGGHDYEGLSY++  PF+I+DL NL+SI VDV+ 
Sbjct: 89  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 148

Query: 141 NVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFSGGGYGLLIRKFGVAADNV 200
              WVQ+GATIGELYY I +K++TL FPAG+  TVGVGGHFSGGGYG L+RK G+AAD+V
Sbjct: 149 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 208

Query: 201 IDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKIKLVPVPPVVTIFKITKNL 260
           IDA  +DA G++ +R+ MGED FWAIRGGGG SF +VL+WKI L+ VP  VT+F +TK  
Sbjct: 209 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 268

Query: 261 EQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGIVLTATFKAFFLGRDEELL 320
           EQ+A  ++H+WQ+VA K  + L +R++L        Q  K +V  A+F   +LG  + LL
Sbjct: 269 EQSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMV-RASFPGLYLGSVKNLL 328

Query: 321 SIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPNTLLDRGFALKGSYKGKSDYVEE 380
            ++ K FPEL + ++DC EMSWIESV++FA+L  ++P  +L +      ++K KSD+V+E
Sbjct: 329 KMVNKEFPELGLEEDDCTEMSWIESVIWFAELG-EEPINVLTKRTRASLAFKAKSDFVQE 388

Query: 381 PISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEIAFPHRAGNLYKIHYTVSWG 440
           P+ K  +  +W  L E   + AQL   P+GG M++I++ E  FPHR GN+Y+I Y   W 
Sbjct: 389 PMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWR 448

Query: 441 GGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVGTNNKNGYTSYEQASIWGVR 500
           G  D+ ++++ W+ + YD ++ FV+K+PR  Y+N RDLD+G       + YE+   WGV+
Sbjct: 449 G--DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVK 505

Query: 501 YFKGNFKRLV 509
           YFK NF+RLV
Sbjct: 509 YFKNNFERLV 505

BLAST of MC03g_new0296 vs. NCBI nr
Match: XP_022152442.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 796 bits (2057), Expect = 1.57e-286
Identity = 395/510 (77.45%), Postives = 445/510 (87.25%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS I+SLAYIFVVLSF+ +ASA+N E F+HC S H PN S SIS IIYTP DPLY SVL
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDS-SISKIIYTPTDPLYFSVL 60

Query: 61  NFTIQNLRFLDD--SNPQIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQNLRFL    S PQ+IVTP QASHIQASIICAK NGFQ+RIRSGGHDYEGLSY+SD
Sbjct: 61  NFSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSD 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VD+INL+SIDVDVEK VAWV+SGATIGELYYRIAEKSKTL FPAGLSSTVGVGGH
Sbjct: 121 VPFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYG LIRKFG+AADNVIDAYFID NGKLHDR+SMGED+FWAIRGG G SFGI+L+W
Sbjct: 181 FSGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGG-GASFGIILSW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           K+KLV VPP+VTIFKIT+NLEQNATDLVH+WQYVA+KQD+RLI+RI+L+A + +N QN K
Sbjct: 241 KVKLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPNTL 360
            IVL A FK FFLG+ EELL IMQ TFPEL + KED IEMSWIES++YFAKLP  Q + L
Sbjct: 301 RIVLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASAL 360

Query: 361 LDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESE 420
           LDR  ALKG+YKGK DYV EPI KAGLEGIWELLNE+ L FAQLD+IPYGG M++ISESE
Sbjct: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420

Query: 421 IAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDV 480
           I FPHRAGN+YKIHYTV++ G  D  QRHI WI KFY+YLTPFVSKNPR  Y+NYRDLD+
Sbjct: 421 IPFPHRAGNIYKIHYTVAYSG--DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDI 480

Query: 481 GTNNKNGYTSYEQASIWGVRYFKGNFKRLV 508
           G NNKNG TSYE+AS+WG++YFKGNFKRLV
Sbjct: 481 GMNNKNGCTSYEKASVWGLKYFKGNFKRLV 506

BLAST of MC03g_new0296 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 758 bits (1958), Expect = 1.88e-271
Identity = 379/513 (73.88%), Postives = 433/513 (84.41%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS ITSLAYIFVVLSF  +ASA N E F+ C S HSPN+S SIS I+YTP D  Y S+L
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNS-SISKIVYTPIDISYFSIL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQNLRF     P  Q+IVTP Q SHIQASIICAK+NGFQ+R RSGGHDYEGLSY+SD
Sbjct: 61  NFSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSD 120

Query: 121 -APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGG 180
             PF+++DL NL+SIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGL STVGVGG
Sbjct: 121 NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGG 180

Query: 181 HFSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLA 240
           HFSGGGYG+++RKFG+AADNVIDAYFID NG L DRKSMGEDVFWAIRGGGG SFGI+LA
Sbjct: 181 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILA 240

Query: 241 WKIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAM-DSSNQQN 300
           WKIKL+PVPPVVT+F IT+NLEQNAT+L+H+WQYVASKQD+RL++R++L+ M +SSN QN
Sbjct: 241 WKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 300

Query: 301 EKGIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPN 360
            K   L A+F + FLG+ +ELL IMQKTFPEL +TKEDCIEMSWIE+VLYFA       N
Sbjct: 301 XKK-TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLN 360

Query: 361 TLLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISE 420
            LLDR  A KG++KGKSDYV EPI KAG+EGIWEL NEE +QFAQ+ +IPYGG M++ISE
Sbjct: 361 VLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE 420

Query: 421 SEIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDL 480
            EI FPHR GNLYKIHY V  G GED AQR ISWI K Y Y+TPFVSKNPR  Y+NYRDL
Sbjct: 421 XEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDL 480

Query: 481 DVGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           D+GTNNK+G TSY++ASIWG++YFK NFKRLVS
Sbjct: 481 DIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVS 511

BLAST of MC03g_new0296 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 744 bits (1920), Expect = 1.05e-265
Identity = 371/512 (72.46%), Postives = 429/512 (83.79%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS I SLAYIFVVLSFA +ASA N E F+HC S H PNSS SIS IIYTP  P YS VL
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSS-SISKIIYTPTHPSYSLVL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQNLRFL    P  QIIVTP +ASH+QASIICAKNNG Q+R RSGGHDYEGLSY+S+
Sbjct: 61  NFSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSN 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VDL NL+SIDVDVE+ VAWVQSGATIGELYYRIAEKSKTLGFPAGL  TVG+GGH
Sbjct: 121 VPFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYG+++RKFG+AADNVIDAYFID NGKLHDRKSMGEDVFWAIRGGGG SFGIVLAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           KIKL+ VP +VT+F IT+NLEQNAT+L+++WQYVA+KQD+RLI++I+L   +S+N +N K
Sbjct: 241 KIKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQP-NT 360
             +  A F + FLG+ E+LL IMQKTFPEL + KEDCIEMSWIESVLYFA+ P  QP N 
Sbjct: 301 RTI-EAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNV 360

Query: 361 LLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISES 420
           LLDR    KGS+KGKSDYV EP+ KAGLEGIWEL NE+  +  Q+ +IPYGG MN+ISES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 421 EIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLD 480
           EI FPHRAGNLY+IHY++  GG E+  QR+  WI KFY Y+ PFVSKNPR  Y+NYRDLD
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLD 480

Query: 481 VGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           +GTNN+NG TSY++ASIWGV+YFK NFKRLVS
Sbjct: 481 IGTNNENGNTSYDKASIWGVKYFKDNFKRLVS 508

BLAST of MC03g_new0296 vs. NCBI nr
Match: XP_022152426.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 740 bits (1911), Expect = 2.95e-264
Identity = 360/513 (70.18%), Postives = 431/513 (84.02%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS ITSLAYIFVV  F+ +ASA+N+E FIHC SLHS N+S SI NIIYT  DP YSS+L
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTS-SILNIIYTSTDPSYSSIL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           N++I N RF+    P  Q+IVTP Q SHIQASIICA+ NGFQ+R RSGGHDYEG+SY+SD
Sbjct: 61  NYSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSD 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VD +NL+SID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+  TVGVGGH
Sbjct: 121 VPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYG+++RKFG+A DNVIDAY +D NG+LHDRKSMGEDVFWAIRGG G SFGI+L+W
Sbjct: 181 FSGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGG-GASFGIILSW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           KIKLV VP VVT+F I +NLE+NATDLVH+WQYVA+KQD+RL++RI+L  M SSN QN K
Sbjct: 241 KIKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKL-PLDQP-N 360
            + + A+F + FLG+ EELL IMQ+TFPEL +TKEDC EMSWIESVLYFA   P  QP +
Sbjct: 301 RLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLD 360

Query: 361 TLLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISE 420
            L+DR FA +G +KGKSDYV+EPI K GL G+WELLN++ +QFA++ +IPYGG MN+ISE
Sbjct: 361 VLIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISE 420

Query: 421 SEIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDL 480
           SE  +PHRAGNLY IHY VSWGG EDIAQ+HISWI K Y+Y+TPFVSKNPR  YVNYRDL
Sbjct: 421 SETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDL 480

Query: 481 DVGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           D+G NN N YTSYE+ASIWG++Y+K NF+RLVS
Sbjct: 481 DLGINNINEYTSYEKASIWGLKYYKDNFERLVS 511

BLAST of MC03g_new0296 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 738 bits (1904), Expect = 3.07e-263
Identity = 367/512 (71.68%), Postives = 426/512 (83.20%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS ITSLAYIF+ LS A +ASA N E F+ C S+HSPNSS  ISNIIYTP DP YSSVL
Sbjct: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSP-ISNIIYTPTDPSYSSVL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQN RFL    P  QIIVTP +ASHIQAS+ICAKNNGFQ+R RSGGHDYEGLSY+SD
Sbjct: 61  NFSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSD 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VDL N++SIDVDVE+NVAWVQSGATIGELYYRIAEKSKTLGFPAGL  TVG+GGH
Sbjct: 121 VPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYGL++RK+G+AADNVIDAYFID NG+LHDRKSMGEDVFWAIRGG G SFGI+LAW
Sbjct: 181 FSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGG-GASFGIILAW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           K+KLV VPPVVT+F IT+NL+QNAT+LVH+WQYVA+KQD+RLI+R +L   +S N QN K
Sbjct: 241 KVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQP-NT 360
             V  A F + FLG+  ELL IMQ TFPEL + KEDCIEMSWIESVLYF+  P +QP N 
Sbjct: 301 RTV-KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 360

Query: 361 LLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISES 420
           L+DR  ALKG +KGKSDY+ +PI KAGLEG+WELL  + ++F ++ ++PYGG MN+ISE+
Sbjct: 361 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 420

Query: 421 EIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLD 480
           +I FPHRA NLY IHY VSW    D  QRHISWI K Y Y+TPFVSKNPR  Y NYRDLD
Sbjct: 421 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 480

Query: 481 VGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           +GTNNK+G T+YE+ASIWG +Y+K NFKRLVS
Sbjct: 481 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVS 508

BLAST of MC03g_new0296 vs. ExPASy TrEMBL
Match: A0A6J1DG99 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020173 PE=3 SV=1)

HSP 1 Score: 796 bits (2057), Expect = 7.60e-287
Identity = 395/510 (77.45%), Postives = 445/510 (87.25%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS I+SLAYIFVVLSF+ +ASA+N E F+HC S H PN S SIS IIYTP DPLY SVL
Sbjct: 1   MKSPISSLAYIFVVLSFSWVASAYNHEDFLHCLSFHFPNDS-SISKIIYTPTDPLYFSVL 60

Query: 61  NFTIQNLRFLDD--SNPQIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQNLRFL    S PQ+IVTP QASHIQASIICAK NGFQ+RIRSGGHDYEGLSY+SD
Sbjct: 61  NFSIQNLRFLGGKTSKPQVIVTPLQASHIQASIICAKKNGFQIRIRSGGHDYEGLSYVSD 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VD+INL+SIDVDVEK VAWV+SGATIGELYYRIAEKSKTL FPAGLSSTVGVGGH
Sbjct: 121 VPFVVVDMINLRSIDVDVEKRVAWVESGATIGELYYRIAEKSKTLAFPAGLSSTVGVGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYG LIRKFG+AADNVIDAYFID NGKLHDR+SMGED+FWAIRGG G SFGI+L+W
Sbjct: 181 FSGGGYGSLIRKFGLAADNVIDAYFIDVNGKLHDRESMGEDLFWAIRGG-GASFGIILSW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           K+KLV VPP+VTIFKIT+NLEQNATDLVH+WQYVA+KQD+RLI+RI+L+A + +N QN K
Sbjct: 241 KVKLVSVPPMVTIFKITRNLEQNATDLVHQWQYVANKQDDRLIIRIILNATNKNNHQNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPNTL 360
            IVL A FK FFLG+ EELL IMQ TFPEL + KED IEMSWIES++YFAKLP  Q + L
Sbjct: 301 RIVLQAAFKGFFLGKVEELLPIMQNTFPELGLRKEDGIEMSWIESIIYFAKLPKGQASAL 360

Query: 361 LDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESE 420
           LDR  ALKG+YKGK DYV EPI KAGLEGIWELLNE+ L FAQLD+IPYGG M++ISESE
Sbjct: 361 LDRSLALKGTYKGKCDYVNEPIPKAGLEGIWELLNEKDLDFAQLDLIPYGGRMDEISESE 420

Query: 421 IAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDV 480
           I FPHRAGN+YKIHYTV++ G  D  QRHI WI KFY+YLTPFVSKNPR  Y+NYRDLD+
Sbjct: 421 IPFPHRAGNIYKIHYTVAYSG--DAEQRHIGWIRKFYNYLTPFVSKNPRATYLNYRDLDI 480

Query: 481 GTNNKNGYTSYEQASIWGVRYFKGNFKRLV 508
           G NNKNG TSYE+AS+WG++YFKGNFKRLV
Sbjct: 481 GMNNKNGCTSYEKASVWGLKYFKGNFKRLV 506

BLAST of MC03g_new0296 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 758 bits (1958), Expect = 9.10e-272
Identity = 379/513 (73.88%), Postives = 433/513 (84.41%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS ITSLAYIFVVLSF  +ASA N E F+ C S HSPN+S SIS I+YTP D  Y S+L
Sbjct: 1   MKSPITSLAYIFVVLSFVWVASASNYEDFLRCLSFHSPNNS-SISKIVYTPIDISYFSIL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQNLRF     P  Q+IVTP Q SHIQASIICAK+NGFQ+R RSGGHDYEGLSY+SD
Sbjct: 61  NFSIQNLRFATAKTPKPQVIVTPLQVSHIQASIICAKSNGFQIRTRSGGHDYEGLSYVSD 120

Query: 121 -APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGG 180
             PF+++DL NL+SIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGL STVGVGG
Sbjct: 121 NVPFVVIDLTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGG 180

Query: 181 HFSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLA 240
           HFSGGGYG+++RKFG+AADNVIDAYFID NG L DRKSMGEDVFWAIRGGGG SFGI+LA
Sbjct: 181 HFSGGGYGVMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILA 240

Query: 241 WKIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAM-DSSNQQN 300
           WKIKL+PVPPVVT+F IT+NLEQNAT+L+H+WQYVASKQD+RL++R++L+ M +SSN QN
Sbjct: 241 WKIKLLPVPPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQN 300

Query: 301 EKGIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPN 360
            K   L A+F + FLG+ +ELL IMQKTFPEL +TKEDCIEMSWIE+VLYFA       N
Sbjct: 301 XKK-TLEASFNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQGQPLN 360

Query: 361 TLLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISE 420
            LLDR  A KG++KGKSDYV EPI KAG+EGIWEL NEE +QFAQ+ +IPYGG M++ISE
Sbjct: 361 VLLDRSLAWKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISE 420

Query: 421 SEIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDL 480
            EI FPHR GNLYKIHY V  G GED AQR ISWI K Y Y+TPFVSKNPR  Y+NYRDL
Sbjct: 421 XEIPFPHRVGNLYKIHYAVLLGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDL 480

Query: 481 DVGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           D+GTNNK+G TSY++ASIWG++YFK NFKRLVS
Sbjct: 481 DIGTNNKDGNTSYDKASIWGMKYFKSNFKRLVS 511

BLAST of MC03g_new0296 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 744 bits (1920), Expect = 5.10e-266
Identity = 371/512 (72.46%), Postives = 429/512 (83.79%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS I SLAYIFVVLSFA +ASA N E F+HC S H PNSS SIS IIYTP  P YS VL
Sbjct: 1   MKSPIISLAYIFVVLSFARVASACNYEDFLHCLSFHFPNSS-SISKIIYTPTHPSYSLVL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQNLRFL    P  QIIVTP +ASH+QASIICAKNNG Q+R RSGGHDYEGLSY+S+
Sbjct: 61  NFSIQNLRFLGAKTPKPQIIVTPLEASHVQASIICAKNNGLQIRTRSGGHDYEGLSYVSN 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VDL NL+SIDVDVE+ VAWVQSGATIGELYYRIAEKSKTLGFPAGL  TVG+GGH
Sbjct: 121 VPFVVVDLTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYG+++RKFG+AADNVIDAYFID NGKLHDRKSMGEDVFWAIRGGGG SFGIVLAW
Sbjct: 181 FSGGGYGVMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           KIKL+ VP +VT+F IT+NLEQNAT+L+++WQYVA+KQD+RLI++I+L   +S+N +N K
Sbjct: 241 KIKLLQVPXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQP-NT 360
             +  A F + FLG+ E+LL IMQKTFPEL + KEDCIEMSWIESVLYFA+ P  QP N 
Sbjct: 301 RTI-EAFFNSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNV 360

Query: 361 LLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISES 420
           LLDR    KGS+KGKSDYV EP+ KAGLEGIWEL NE+  +  Q+ +IPYGG MN+ISES
Sbjct: 361 LLDRRSTFKGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISES 420

Query: 421 EIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLD 480
           EI FPHRAGNLY+IHY++  GG E+  QR+  WI KFY Y+ PFVSKNPR  Y+NYRDLD
Sbjct: 421 EIPFPHRAGNLYQIHYSIYCGGDEE--QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLD 480

Query: 481 VGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           +GTNN+NG TSY++ASIWGV+YFK NFKRLVS
Sbjct: 481 IGTNNENGNTSYDKASIWGVKYFKDNFKRLVS 508

BLAST of MC03g_new0296 vs. ExPASy TrEMBL
Match: A0A6J1DHP3 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020162 PE=3 SV=1)

HSP 1 Score: 740 bits (1911), Expect = 1.43e-264
Identity = 360/513 (70.18%), Postives = 431/513 (84.02%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS ITSLAYIFVV  F+ +ASA+N+E FIHC SLHS N+S SI NIIYT  DP YSS+L
Sbjct: 1   MKSPITSLAYIFVVSWFSWVASAYNQENFIHCLSLHSLNTS-SILNIIYTSTDPSYSSIL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           N++I N RF+    P  Q+IVTP Q SHIQASIICA+ NGFQ+R RSGGHDYEG+SY+SD
Sbjct: 61  NYSIMNERFMGAKTPKPQVIVTPLQVSHIQASIICARKNGFQIRTRSGGHDYEGISYVSD 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VD +NL+SID+DVE+ VAWVQSGAT+GELYY+IAEKSKTLG+P G+  TVGVGGH
Sbjct: 121 VPFVLVDFVNLRSIDIDVERKVAWVQSGATLGELYYKIAEKSKTLGYPGGVCPTVGVGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYG+++RKFG+A DNVIDAY +D NG+LHDRKSMGEDVFWAIRGG G SFGI+L+W
Sbjct: 181 FSGGGYGVMLRKFGLAIDNVIDAYLVDVNGELHDRKSMGEDVFWAIRGG-GASFGIILSW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           KIKLV VP VVT+F I +NLE+NATDLVH+WQYVA+KQD+RL++RI+L  M SSN QN K
Sbjct: 241 KIKLVSVPSVVTVFTIKRNLEENATDLVHRWQYVANKQDDRLVIRIILSGMKSSNHQNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKL-PLDQP-N 360
            + + A+F + FLG+ EELL IMQ+TFPEL +TKEDC EMSWIESVLYFA   P  QP +
Sbjct: 301 RLSIEASFSSLFLGKVEELLPIMQRTFPELGMTKEDCTEMSWIESVLYFADFEPKRQPLD 360

Query: 361 TLLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISE 420
            L+DR FA +G +KGKSDYV+EPI K GL G+WELLN++ +QFA++ +IPYGG MN+ISE
Sbjct: 361 VLIDRSFAFRGVFKGKSDYVDEPIPKTGLIGLWELLNQKEVQFARMHLIPYGGKMNEISE 420

Query: 421 SEIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDL 480
           SE  +PHRAGNLY IHY VSWGG EDIAQ+HISWI K Y+Y+TPFVSKNPR  YVNYRDL
Sbjct: 421 SETPYPHRAGNLYNIHYVVSWGGDEDIAQKHISWIRKLYNYMTPFVSKNPRGAYVNYRDL 480

Query: 481 DVGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           D+G NN N YTSYE+ASIWG++Y+K NF+RLVS
Sbjct: 481 DLGINNINEYTSYEKASIWGLKYYKDNFERLVS 511

BLAST of MC03g_new0296 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 738 bits (1904), Expect = 1.49e-263
Identity = 367/512 (71.68%), Postives = 426/512 (83.20%), Query Frame = 0

Query: 1   MKSTITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVL 60
           MKS ITSLAYIF+ LS A +ASA N E F+ C S+HSPNSS  ISNIIYTP DP YSSVL
Sbjct: 1   MKSPITSLAYIFIFLS-AWVASACNHEDFLDCLSIHSPNSSP-ISNIIYTPTDPSYSSVL 60

Query: 61  NFTIQNLRFLDDSNP--QIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSD 120
           NF+IQN RFL    P  QIIVTP +ASHIQAS+ICAKNNGFQ+R RSGGHDYEGLSY+SD
Sbjct: 61  NFSIQNQRFLGAKTPKPQIIVTPLEASHIQASVICAKNNGFQIRTRSGGHDYEGLSYVSD 120

Query: 121 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 180
            PF++VDL N++SIDVDVE+NVAWVQSGATIGELYYRIAEKSKTLGFPAGL  TVG+GGH
Sbjct: 121 VPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGH 180

Query: 181 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 240
           FSGGGYGL++RK+G+AADNVIDAYFID NG+LHDRKSMGEDVFWAIRGG G SFGI+LAW
Sbjct: 181 FSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAIRGG-GASFGIILAW 240

Query: 241 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 300
           K+KLV VPPVVT+F IT+NL+QNAT+LVH+WQYVA+KQD+RLI+R +L   +S N QN K
Sbjct: 241 KVKLVLVPPVVTVFSITRNLKQNATNLVHRWQYVANKQDDRLIIRAVLGVTNSKNHQNGK 300

Query: 301 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQP-NT 360
             V  A F + FLG+  ELL IMQ TFPEL + KEDCIEMSWIESVLYF+  P +QP N 
Sbjct: 301 RTV-KANFNSLFLGKVGELLPIMQNTFPELGLKKEDCIEMSWIESVLYFSDFPREQPLNV 360

Query: 361 LLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISES 420
           L+DR  ALKG +KGKSDY+ +PI KAGLEG+WELL  + ++F ++ ++PYGG MN+ISE+
Sbjct: 361 LIDRNLALKGIFKGKSDYINQPIPKAGLEGLWELLINDEVEFVRMHLVPYGGKMNEISEA 420

Query: 421 EIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLD 480
           +I FPHRA NLY IHY VSW    D  QRHISWI K Y Y+TPFVSKNPR  Y NYRDLD
Sbjct: 421 DIPFPHRAENLYMIHYVVSWTEVGDSEQRHISWIRKLYRYMTPFVSKNPRAAYFNYRDLD 480

Query: 481 VGTNNKNGYTSYEQASIWGVRYFKGNFKRLVS 509
           +GTNNK+G T+YE+ASIWG +Y+K NFKRLVS
Sbjct: 481 IGTNNKDGNTNYEKASIWGAKYYKDNFKRLVS 508

BLAST of MC03g_new0296 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 492.3 bits (1266), Expect = 4.8e-139
Identity = 248/513 (48.34%), Postives = 345/513 (67.25%), Query Frame = 0

Query: 7   SLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTP--RDPLYSSVLNFTI 66
           +L  + ++L    L+S+  ++ F+ C  + + + S  I+   ++P     L+   L  T 
Sbjct: 11  TLFLVTLLLISVPLSSSTLQQDFVKCL-VDNSDVSFPITASFFSPDQNATLFKEELESTA 70

Query: 67  QNLRFLDDSNPQ--IIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLS--DAP 126
           QNLR+L  SNP+   I  P   +H+QA+++CAK     +R+RSGGHDYEGLS+++  + P
Sbjct: 71  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 130

Query: 127 FLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFS 186
           F+IVDL  L+ +DVD++ N AW  +GATIGE+YYRI EKS+T GFPAGL S++G+GGH  
Sbjct: 131 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 190

Query: 187 GGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKI 246
           GG YG ++RKFG+ ADNV+DA  +DANG++ DR +MGEDVFWAIRGGGGGSFG++LAWKI
Sbjct: 191 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 250

Query: 247 KLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGI 306
           KLVPVP  VT+F +TK LEQ+ T +++KW+ +A K D+ L +R+++     + +   +  
Sbjct: 251 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNR-- 310

Query: 307 VLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLD-QPNTLL 366
            ++ +++A FLG    LL +MQK+FPEL +TK+DC EMSWI+SV+Y A  P    P  LL
Sbjct: 311 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL 370

Query: 367 DRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEI 426
                 K  +K KSD+V+EPI   GLEG+WE   EE       +  PYGG M++ISESEI
Sbjct: 371 AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWN--PYGGMMSRISESEI 430

Query: 427 AFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVG 486
            FPHR G L+KI +  +W  G+   +RH+ WI + Y Y+  +VSKNPR  YVNYRDLD+G
Sbjct: 431 PFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLG 490

Query: 487 TNNKNGYTSYEQASIWGVRYFKGNFKRLVSEGG 513
           TN   G T    A  WG +Y+KGNF+RLV   G
Sbjct: 491 TN--EGET---DAREWGAKYYKGNFERLVKIKG 513

BLAST of MC03g_new0296 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 490.0 bits (1260), Expect = 2.4e-138
Identity = 247/507 (48.72%), Postives = 341/507 (67.26%), Query Frame = 0

Query: 11  IFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNIIYTPRD--PLYSSVLNFTIQNLR 70
           ++ +   +SLAS   ++ FI+C    + +    +    + P     ++S VL  T QNLR
Sbjct: 19  LYTITPTSSLASL--QDQFINCVQ-RNTHVYFPLEKTFFAPTKNVSMFSQVLESTAQNLR 78

Query: 71  FLDDSNPQ--IIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLS--DAPFLIV 130
           FL  S P+   I +P   SH+QASIIC+K     +R+RSGGHDYEGLSY+S  D PF+++
Sbjct: 79  FLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILM 138

Query: 131 DLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFSGGGY 190
           DL  ++ ++++++ N AWVQSGAT+GELYYRIAEKSK  GFPAGL S++G+GGH +GG Y
Sbjct: 139 DLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAY 198

Query: 191 GLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKIKLVP 250
           G ++RK+G+ ADNV+DA  +DANGKL DR +MGED FWAIRGG GGSFGI+LAWKIKLVP
Sbjct: 199 GSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVP 258

Query: 251 VPPVVTIFKITKNLEQN-ATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGIVLT 310
           VP  VT+F +TK L+Q+    ++ KWQ VA K  E L +R+L +   +   +      +T
Sbjct: 259 VPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK-----TVT 318

Query: 311 ATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPNTLLDRGF 370
            ++ A FLG    L+++M+K+FPEL +T +DCIEMSW+ES+ Y +  P   P  +L +G 
Sbjct: 319 TSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGK 378

Query: 371 A--LKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEIAF 430
           +   K S+K KSD+V+ PI ++GL+GI++ L +E +     +  PYGG M KI ES+I F
Sbjct: 379 SPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPLMIWN--PYGGMMAKIPESQIPF 438

Query: 431 PHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVGTN 490
           PHR G L+K+ Y  SW   +    RHI+WI   Y Y+TP+VS NPR  YVNYRDLD+G N
Sbjct: 439 PHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRN 498

Query: 491 NKNGYTSYEQASIWGVRYFKGNFKRLV 509
            K+  T  +QA +WG  YFK NF RL+
Sbjct: 499 TKDVKTCIKQAQVWGANYFKNNFNRLM 515

BLAST of MC03g_new0296 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.7e-136
Identity = 245/511 (47.95%), Postives = 339/511 (66.34%), Query Frame = 0

Query: 4   TITSLAYIFVVLSFASLASAFNREGFIHCFSLHSPNSSDSISNII-YTPRDPLYSSVLNF 63
           T+  L+  ++ LSF+++     ++ F+ C   +S  S    S+   +      +   L  
Sbjct: 15  TLLLLSLSYIPLSFSTI-----QQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALET 74

Query: 64  TIQNLRFLDDSN--PQIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLS--D 123
           + QNLR+L  SN  P+ I  P   +H+QA+++CAK     +R+RSGGHDYEGLSY+S  +
Sbjct: 75  SAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEME 134

Query: 124 APFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGH 183
             F+IVDL  L+ I VD+E N AWV +GA+IGE+YYRI EKSK  GFPAGL +++G+GGH
Sbjct: 135 TAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGH 194

Query: 184 FSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAW 243
             GG YG ++RKFG+ ADNV+DA  +DA+GK+ +R +MGEDVFWAIRGGGGGSFG++LAW
Sbjct: 195 IIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAW 254

Query: 244 KIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEK 303
           KIKLVPVP +VT+F +T+ LEQ+ T L++KWQ VA K DE L +R+++     + +  E+
Sbjct: 255 KIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKER 314

Query: 304 GIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPN-T 363
              ++ +++  FLG    LL +MQ++FP+L +TK+DC+E SWI+SV+Y A  P   P+  
Sbjct: 315 --TISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEA 374

Query: 364 LLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISES 423
           LLD     K  +K KSDYVEEPI   GLEG+WE L EE       +  PYGG M KI E+
Sbjct: 375 LLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWN--PYGGMMAKIPET 434

Query: 424 EIAFPHRAGNLYKIHYTVSWGGGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLD 483
           E  FPHR+G L+KI +   W  G+    +H+ W+ + Y Y+  +VSK+PR+ YVNYRDLD
Sbjct: 435 ETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLD 494

Query: 484 VGTNNKNGYTSYEQASIWGVRYFKGNFKRLV 509
           +G N K        A  WG RYFKGNF+RLV
Sbjct: 495 LGMNGKG-----SDAREWGNRYFKGNFERLV 511

BLAST of MC03g_new0296 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 480.3 bits (1235), Expect = 1.9e-135
Identity = 236/490 (48.16%), Postives = 334/490 (68.16%), Query Frame = 0

Query: 21  ASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSSVLNFTIQNLRFL--DDSNPQII 80
           A+  N+  F+ C SL   N S+ +S +I+TP D  +SSVL  +IQN RF   D   P +I
Sbjct: 29  ANRSNQSSFLQCLSLQL-NDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLI 88

Query: 81  VTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYLSDAPFLIVDLINLQSIDVDVEK 140
           +TP Q S +Q+++ CA+  G  +R RSGGHDYEGLSY++  PF+I+DL NL+SI VDV+ 
Sbjct: 89  LTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDN 148

Query: 141 NVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGVGGHFSGGGYGLLIRKFGVAADNV 200
              WVQ+GATIGELYY I +K++TL FPAG+  TVGVGGHFSGGGYG L+RK G+AAD+V
Sbjct: 149 RSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHV 208

Query: 201 IDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIVLAWKIKLVPVPPVVTIFKITKNL 260
           IDA  +DA G++ +R+ MGED FWAIRGGGG SF +VL+WKI L+ VP  VT+F +TK  
Sbjct: 209 IDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTKFS 268

Query: 261 EQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQNEKGIVLTATFKAFFLGRDEELL 320
           EQ+A  ++H+WQ+VA K  + L +R++L        Q  K +V  A+F   +LG  + LL
Sbjct: 269 EQSALKIIHRWQFVADKVSDDLFIRVML--------QRYKNMV-RASFPGLYLGSVKNLL 328

Query: 321 SIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQPNTLLDRGFALKGSYKGKSDYVEE 380
            ++ K FPEL + ++DC EMSWIESV++FA+L  ++P  +L +      ++K KSD+V+E
Sbjct: 329 KMVNKEFPELGLEEDDCTEMSWIESVIWFAELG-EEPINVLTKRTRASLAFKAKSDFVQE 388

Query: 381 PISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKISESEIAFPHRAGNLYKIHYTVSWG 440
           P+ K  +  +W  L E   + AQL   P+GG M++I++ E  FPHR GN+Y+I Y   W 
Sbjct: 389 PMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWR 448

Query: 441 GGEDIAQRHISWINKFYDYLTPFVSKNPRTLYVNYRDLDVGTNNKNGYTSYEQASIWGVR 500
           G  D+ ++++ W+ + YD ++ FV+K+PR  Y+N RDLD+G       + YE+   WGV+
Sbjct: 449 G--DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVK 505

Query: 501 YFKGNFKRLV 509
           YFK NF+RLV
Sbjct: 509 YFKNNFERLV 505

BLAST of MC03g_new0296 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.6e-134
Identity = 244/518 (47.10%), Postives = 342/518 (66.02%), Query Frame = 0

Query: 5   ITSLAYIFVVLS-----FASL-ASAFNREGFIHCFSLHSPNSSDSISNIIYTPRDPLYSS 64
           + SL   F++L+     F S+ A   N E F+ C S H  N  D  S II+T +DP Y S
Sbjct: 7   LLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLS-HRINEDD--SRIIHTSKDPSYFS 66

Query: 65  VLNFTIQNLRF--LDDSNPQIIVTPFQASHIQASIICAKNNGFQVRIRSGGHDYEGLSYL 124
           +LN +IQN RF  L+   P  I+TP QA+ +Q++I CA+ +G  +R RSGGHDYEGLSY+
Sbjct: 67  ILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYM 126

Query: 125 SDA-PFLIVDLINLQSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLSSTVGV 184
           + + PF+++DL NL+SI +DV+    WVQSGATIGELYY I + SK+L FPAGL  TVG+
Sbjct: 127 AKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGI 186

Query: 185 GGHFSGGGYGLLIRKFGVAADNVIDAYFIDANGKLHDRKSMGEDVFWAIRGGGGGSFGIV 244
           GG F GGGYG L+RK+G++ADNVIDA+ +DANG   DR+ MGED FWAIRGGGG SF +V
Sbjct: 187 GGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVV 246

Query: 245 LAWKIKLVPVPPVVTIFKITKNLEQNATDLVHKWQYVASKQDERLILRILLDAMDSSNQQ 304
           L+WKI+L+ VP VVT+FK+ K  E+ A  +++KWQY+A K    L +R +L         
Sbjct: 247 LSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML--------- 306

Query: 305 NEKGIVLTATFKAFFLGRDEELLSIMQKTFPELRVTKEDCIEMSWIESVLYFAKLPLDQP 364
            +K   + A+F   +LG   +LL++M+  FPEL +   +C EMSWIESVL+F K    + 
Sbjct: 307 -QKETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIK---GES 366

Query: 365 NTLLDRGFALKGSYKGKSDYVEEPISKAGLEGIWELLNEEGLQFAQLDIIPYGGTMNKIS 424
             +L +      S+KGK D++EEPI K  ++ +W        + A++ + P+GG M++I+
Sbjct: 367 MEILAKRKRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 426

Query: 425 ESEIAFPHRAGNLYKIHYTVSWGGGED----IAQRHISWINKFYDYLTPFVSKNPRTLYV 484
           ++EI FPHR GNLY+I Y   W   ED      ++++ W+   Y+++TP+VSK+PR  YV
Sbjct: 427 DNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYV 486

Query: 485 NYRDLDVGTN-NKNGYTSYEQASIWGVRYFKGNFKRLV 509
           N+RD+D+G     N  T YE+A +WGV+YFK NF RLV
Sbjct: 487 NFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLV 508