MC03g_new0288 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0288
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	FAD-binding Berberine family protein
Location	MC03: 12874518 .. 12875096 (-)
RNA-Seq Expression	MC03g_new0288
Synteny	MC03g_new0288

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGATTTCATCCATTGTCTTTCTTTTCATTTCCCCAACAGCTATTCAATTTCAAAGATCATTTACCGCCCAAGTGATCCTTTTCATACTCTTCAATTTTGGACTTTTCAATCCAGAATCTAAAATTCTTGGATGTCAAAACTCCGAAGCCTCAAGTAATGGTGACGCCATTGCAAGTTTCCCACATTCAAGCATCAATCATTTGTGCAAAAAAACACGACTTCCAGATACGAACACGAAGCGGTGGCCTGACTACGAGGGTCTTTCTTTTGTCTCTGATGTTCCTTTCGTCCTCCTTGATATGATGAATCTCCGATCGATAAACGTGGATGTGGAAAACAATATTGCATGGGTGCAATCTGGAGCCATCGGCGAACTCTACTATAGAATTGCAGAGAAAAGCAAAACACTAGGGTTCCTAGTTGGGCTTTGTCCGACAGTCGGCATTGGTGGGCACTTCAGTGGTGGTGGCAATGGTGTGATGCTACGAAAATTTGGCCTCGCTGCCGATAACGTGATGTCAACGATAATCTCCATGACCGAAAATCAATGGGAGGATATATTTTGGGCCATTTAG

mRNA sequence

ATGAAGATTTCATCCATTGTCTTTCTTTTCATTTCCCCAACAGCTATTCAATTTCAAAGATCATTTACCGCCCAAGTGATCCTTTTCATACTCTTCAATTTTGGACTTTTCAATCCAGAATCTAAAATTCTTGGATGTCAAAACTCCGAAGCCTCAAGTAATGGTGACGCCATTGCAAGTTTCCCACATTCAAGCATCAATCATTTGTGCAAAAAAACACGACTTCCAGATACGAACACGAAGCGGTGGCCTGACTACGAGGGTCTTTCTTTTGTCTCTGATGTTCCTTTCGTCCTCCTTGATATGATGAATCTCCGATCGATAAACGTGGATGTGGAAAACAATATTGCATGGGTGCAATCTGGAGCCATCGGCGAACTCTACTATAGAATTGCAGAGAAAAGCAAAACACTAGGGTTCCTAGTTGGGCTTTGTCCGACAGTCGGCATTGGTGGGCACTTCAGTGGTGGTGGCAATGGTGTGATGCTACGAAAATTTGGCCTCGCTGCCGATAACGTGATGTCAACGATAATCTCCATGACCGAAAATCAATGGGAGGATATATTTTGGGCCATTTAG

Coding sequence (CDS)

ATGAAGATTTCATCCATTGTCTTTCTTTTCATTTCCCCAACAGCTATTCAATTTCAAAGATCATTTACCGCCCAAGTGATCCTTTTCATACTCTTCAATTTTGGACTTTTCAATCCAGAATCTAAAATTCTTGGATGTCAAAACTCCGAAGCCTCAAGTAATGGTGACGCCATTGCAAGTTTCCCACATTCAAGCATCAATCATTTGTGCAAAAAAACACGACTTCCAGATACGAACACGAAGCGGTGGCCTGACTACGAGGGTCTTTCTTTTGTCTCTGATGTTCCTTTCGTCCTCCTTGATATGATGAATCTCCGATCGATAAACGTGGATGTGGAAAACAATATTGCATGGGTGCAATCTGGAGCCATCGGCGAACTCTACTATAGAATTGCAGAGAAAAGCAAAACACTAGGGTTCCTAGTTGGGCTTTGTCCGACAGTCGGCATTGGTGGGCACTTCAGTGGTGGTGGCAATGGTGTGATGCTACGAAAATTTGGCCTCGCTGCCGATAACGTGATGTCAACGATAATCTCCATGACCGAAAATCAATGGGAGGATATATTTTGGGCCATTTAG

Protein sequence

MKISSIVFLFISPTAIQFQRSFTAQVILFILFNFGLFNPESKILGCQNSEASSNGDAIASFPHSSINHLCKKTRLPDTNTKRWPDYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGAIGELYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMTENQWEDIFWAI

Homology

[Close]

BLAST of MC03g_new0288 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 6.0e-31
Identity = 68/116 (58.62%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+V+  PFV+LD+ NLRSI VDV+N   WVQ+GA IGELYY I +K++TL F  G
Sbjct: 118 DYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAG 177

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMST-------IISMTENQWEDIFWAI 193
           +CPTVG+GGHFSGGG G +LRK GLAAD+V+          I       ED FWAI
Sbjct: 178 VCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAI 233

[Close]

BLAST of MC03g_new0288 vs. ExPASy Swiss-Prot
Match: P93479 (Reticuline oxidase OS=Papaver somniferum OX=3469 GN=BBE1 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 7.9e-31
Identity = 70/137 (51.09%), Postives = 87/137 (63.50%), Query Frame = 0


Query: 64  SSINHLCKKTRLPDTNTKRWPDYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA 123
           SS  H C +             YEGLS+ +D PFV++DMMNL  I++DV +  AWV+SGA
Sbjct: 88  SSTVHCCTRESWTIRLRSGGHSYEGLSYTADTPFVIVDMMNLNRISIDVLSETAWVESGA 147

Query: 124 -IGELYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTI----- 183
            +GELYY IA+ + TLGF  G CPTVG GGH SGGG G+M RK+GLAADNV+  I     
Sbjct: 148 TLGELYYAIAQSTDTLGFTAGWCPTVGSGGHISGGGFGMMSRKYGLAADNVVDAILIDSN 207

Query: 184 --ISMTENQWEDIFWAI 193
             I   E   +D+FWAI
Sbjct: 208 GAILDREKMGDDVFWAI 224

[Close]

BLAST of MC03g_new0288 vs. ExPASy Swiss-Prot
Match: Q9SVG3 (Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.8e-30
Identity = 65/116 (56.03%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS++SD PF +LDM NLR ++VD+ +  AW+ +GA +GE+YYRI EKSK  GF  G
Sbjct: 120 DYEGLSYISDKPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAG 179

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMTENQ-------WEDIFWAI 193
           +CPTVG+GGH SGGG G MLRKFGL+ DN++   I     Q        ED+FWAI
Sbjct: 180 VCPTVGVGGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAI 235

[Close]

BLAST of MC03g_new0288 vs. ExPASy Swiss-Prot
Match: P30986 (Reticuline oxidase OS=Eschscholzia californica OX=3467 GN=BBE1 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 2.3e-30
Identity = 64/115 (55.65%), Postives = 84/115 (73.04%), Query Frame = 0


Query: 86  YEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVGL 145
           YEGLS+ SD PF+L+D+MNL  +++D+E+  AWV+SG+ +GELYY I E S  LGF  G 
Sbjct: 106 YEGLSYTSDTPFILIDLMNLNRVSIDLESETAWVESGSTLGELYYAITESSSKLGFTAGW 165

Query: 146 CPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTII-----SMTENQ--WEDIFWAI 193
           CPTVG GGH SGGG G+M RK+GLAADNV+  I+     ++ + Q   ED+FWAI
Sbjct: 166 CPTVGTGGHISGGGFGMMSRKYGLAADNVVDAILIDANGAILDRQAMGEDVFWAI 220

[Close]

BLAST of MC03g_new0288 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.3e-29
Identity = 69/141 (48.94%), Postives = 91/141 (64.54%), Query Frame = 0


Query: 63  HSSINHLC-KKTRLPDTNTKRWPDYEGLSFVS--DVPFVLLDMMNLRSINVDVENNIAWV 122
           H   + +C KK R+         DYEGLS+VS  D PF+L+D+  +R +N+++++N AWV
Sbjct: 95  HVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWV 154

Query: 123 QSGA-IGELYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTII 182
           QSGA +GELYYRIAEKSK  GF  GLC ++GIGGH +GG  G M+RK+GL ADNV+   I
Sbjct: 155 QSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKI 214

Query: 183 SMTENQ-------WEDIFWAI 193
                +        ED FWAI
Sbjct: 215 VDANGKLLDRAAMGEDTFWAI 235

[Close]

BLAST of MC03g_new0288 vs. NCBI nr
Match: XP_038895082.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 166 bits (419), Expect = 1.07e-44
Identity = 82/116 (70.69%), Postives = 97/116 (83.62%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+VSDVPFV++D++NLRSI+VDVENN+AWVQSGA IGELYYRIAEKSKTLGF  G
Sbjct: 110 DYEGLSYVSDVPFVVVDLINLRSISVDVENNVAWVQSGATIGELYYRIAEKSKTLGFPAG 169

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTI-------ISMTENQWEDIFWAI 192
           +CPTVG+GGHFSGGG G+MLRKFGLAADNV+          +   ++  ED+FWAI
Sbjct: 170 VCPTVGVGGHFSGGGYGLMLRKFGLAADNVIDAYFIDVDGKLHDRKSMGEDVFWAI 225

[Close]

BLAST of MC03g_new0288 vs. NCBI nr
Match: OVA08657.1 (FAD linked oxidase [Macleaya cordata])

HSP 1 Score: 155 bits (393), Expect = 6.18e-44
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+VSDVPFV++D+ NL+SI+VD E+  AWVQSGA IGELYYRIAEKS+TLGF VG
Sbjct: 14  DYEGLSYVSDVPFVIVDLFNLQSISVDAEDRNAWVQSGATIGELYYRIAEKSRTLGFPVG 73

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMT-------ENQWEDIFWAI 192
            CPTVG+GGHFSGGG G MLRK+GLAADNV+   I          E+  ED+FWAI
Sbjct: 74  ACPTVGVGGHFSGGGYGTMLRKYGLAADNVIDARIVNVYGRILNKESMGEDLFWAI 129

[Close]

BLAST of MC03g_new0288 vs. NCBI nr
Match: XP_022152520.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 163 bits (413), Expect = 7.58e-44
Identity = 82/116 (70.69%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+VSDVPFVL+D+ N+RSI+VDVE N+AWVQSGA IGELYYRIAEKSKTLGF  G
Sbjct: 109 DYEGLSYVSDVPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAG 168

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTI-------ISMTENQWEDIFWAI 192
           LCPTVGIGGHFSGGG G+MLRK+GLAADNV+          +   ++  ED+FWAI
Sbjct: 169 LCPTVGIGGHFSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAI 224

[Close]

BLAST of MC03g_new0288 vs. NCBI nr
Match: XP_022994776.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 162 bits (411), Expect = 1.52e-43
Identity = 84/133 (63.16%), Postives = 101/133 (75.94%), Query Frame = 0


Query: 69  LCKKTRLPDTNTKRWP-DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGE 128
           +C KT      T+    DYEGLS+VSDVPF+++D++NLRSINVD +NN+AWVQSGA IGE
Sbjct: 93  ICAKTTGFQIRTRSGGHDYEGLSYVSDVPFIIVDLINLRSINVDAKNNVAWVQSGATIGE 152

Query: 129 LYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMST-IISMTEN-- 188
           LYYRIAEKSKTLGF  G+CPTVG+GGHFSGGG G+MLRKFGLA DNV+    I +  N  
Sbjct: 153 LYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLH 212

Query: 189 ----QWEDIFWAI 192
                 ED+FWA+
Sbjct: 213 DRKSMGEDVFWAL 225

[Close]

BLAST of MC03g_new0288 vs. NCBI nr
Match: XP_023542351.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 162 bits (410), Expect = 2.12e-43
Identity = 84/133 (63.16%), Postives = 101/133 (75.94%), Query Frame = 0


Query: 69  LCKKTRLPDTNTKRWP-DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGE 128
           +C KT      T+    DYEGLS+VSDVPF+++D++NLRSINVD +NN+AWVQSGA IGE
Sbjct: 93  ICAKTTGFQIRTRSGGHDYEGLSYVSDVPFIVVDLINLRSINVDAKNNVAWVQSGATIGE 152

Query: 129 LYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMST-IISMTEN-- 188
           LYYRIAEKSKTLGF  G+CPTVG+GGHFSGGG G+MLRKFGLA DNV+    I +  N  
Sbjct: 153 LYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLH 212

Query: 189 ----QWEDIFWAI 192
                 ED+FWA+
Sbjct: 213 DRKSMGEDVFWAL 225

[Close]

BLAST of MC03g_new0288 vs. ExPASy TrEMBL
Match: A0A200QE33 (FAD linked oxidase OS=Macleaya cordata OX=56857 GN=BVC80_1611g5 PE=4 SV=1)

HSP 1 Score: 155 bits (393), Expect = 2.99e-44
Identity = 79/116 (68.10%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+VSDVPFV++D+ NL+SI+VD E+  AWVQSGA IGELYYRIAEKS+TLGF VG
Sbjct: 14  DYEGLSYVSDVPFVIVDLFNLQSISVDAEDRNAWVQSGATIGELYYRIAEKSRTLGFPVG 73

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMT-------ENQWEDIFWAI 192
            CPTVG+GGHFSGGG G MLRK+GLAADNV+   I          E+  ED+FWAI
Sbjct: 74  ACPTVGVGGHFSGGGYGTMLRKYGLAADNVIDARIVNVYGRILNKESMGEDLFWAI 129

[Close]

BLAST of MC03g_new0288 vs. ExPASy TrEMBL
Match: A0A6J1DE62 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 PE=3 SV=1)

HSP 1 Score: 163 bits (413), Expect = 3.67e-44
Identity = 82/116 (70.69%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+VSDVPFVL+D+ N+RSI+VDVE N+AWVQSGA IGELYYRIAEKSKTLGF  G
Sbjct: 109 DYEGLSYVSDVPFVLVDLTNIRSIDVDVEQNVAWVQSGATIGELYYRIAEKSKTLGFPAG 168

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTI-------ISMTENQWEDIFWAI 192
           LCPTVGIGGHFSGGG G+MLRK+GLAADNV+          +   ++  ED+FWAI
Sbjct: 169 LCPTVGIGGHFSGGGYGLMLRKYGLAADNVIDAYFIDVNGELHDRKSMGEDVFWAI 224

[Close]

BLAST of MC03g_new0288 vs. ExPASy TrEMBL
Match: A0A6J1K043 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3 SV=1)

HSP 1 Score: 162 bits (411), Expect = 7.35e-44
Identity = 84/133 (63.16%), Postives = 101/133 (75.94%), Query Frame = 0


Query: 69  LCKKTRLPDTNTKRWP-DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGE 128
           +C KT      T+    DYEGLS+VSDVPF+++D++NLRSINVD +NN+AWVQSGA IGE
Sbjct: 93  ICAKTTGFQIRTRSGGHDYEGLSYVSDVPFIIVDLINLRSINVDAKNNVAWVQSGATIGE 152

Query: 129 LYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMST-IISMTEN-- 188
           LYYRIAEKSKTLGF  G+CPTVG+GGHFSGGG G+MLRKFGLA DNV+    I +  N  
Sbjct: 153 LYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLH 212

Query: 189 ----QWEDIFWAI 192
                 ED+FWA+
Sbjct: 213 DRKSMGEDVFWAL 225

[Close]

BLAST of MC03g_new0288 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 162 bits (410), Expect = 1.03e-43
Identity = 84/133 (63.16%), Postives = 101/133 (75.94%), Query Frame = 0


Query: 69  LCKKTRLPDTNTKRWP-DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGE 128
           +C KT      T+    DYEGLS+VSDVPF+++D++NLRSINVD ENN+AWVQSGA IGE
Sbjct: 93  ICAKTTGFQIRTRSGGHDYEGLSYVSDVPFIVVDLINLRSINVDAENNVAWVQSGATIGE 152

Query: 129 LYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMST-IISMTEN-- 188
           LYYRIA+KSKTLGF  G+CPTVG+GGHFSGGG G+MLRKFGLA DNV+    I +  N  
Sbjct: 153 LYYRIAKKSKTLGFPAGVCPTVGVGGHFSGGGYGLMLRKFGLAVDNVIDAYFIDVNGNLH 212

Query: 189 ----QWEDIFWAI 192
                 ED+FWA+
Sbjct: 213 DRKSMGEDVFWAL 225

[Close]

BLAST of MC03g_new0288 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 161 bits (408), Expect = 1.88e-43
Identity = 86/139 (61.87%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 63  HSSINHLCKKTRLPDTNTKRWP-DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQS 122
           H   + +C K       T+    DYEGLS+VS+VPFV++D+ NLRSI+VDVE  +AWVQS
Sbjct: 87  HVQASIICAKNNGLQIRTRSGGHDYEGLSYVSNVPFVVVDLTNLRSIDVDVEQKVAWVQS 146

Query: 123 GA-IGELYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTI--- 182
           GA IGELYYRIAEKSKTLGF  GLCPTVGIGGHFSGGG GVMLRKFGLAADNV+      
Sbjct: 147 GATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYGVMLRKFGLAADNVIDAYFID 206

Query: 183 ----ISMTENQWEDIFWAI 192
               +   ++  ED+FWAI
Sbjct: 207 VNGKLHDRKSMGEDVFWAI 225

[Close]

BLAST of MC03g_new0288 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 135.6 bits (340), Expect = 4.3e-32
Identity = 68/116 (58.62%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS+V+  PFV+LD+ NLRSI VDV+N   WVQ+GA IGELYY I +K++TL F  G
Sbjct: 118 DYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAG 177

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMST-------IISMTENQWEDIFWAI 193
           +CPTVG+GGHFSGGG G +LRK GLAAD+V+          I       ED FWAI
Sbjct: 178 VCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAI 233

[Close]

BLAST of MC03g_new0288 vs. TAIR 10
Match: AT4G20840.1 (FAD-binding Berberine family protein )

HSP 1 Score: 134.0 bits (336), Expect = 1.2e-31
Identity = 65/116 (56.03%), Postives = 82/116 (70.69%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DYEGLS++SD PF +LDM NLR ++VD+ +  AW+ +GA +GE+YYRI EKSK  GF  G
Sbjct: 120 DYEGLSYISDKPFFILDMSNLRDVSVDIADQSAWISAGATLGEVYYRIWEKSKVHGFPAG 179

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMTENQ-------WEDIFWAI 193
           +CPTVG+GGH SGGG G MLRKFGL+ DN++   I     Q        ED+FWAI
Sbjct: 180 VCPTVGVGGHISGGGYGNMLRKFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAI 235

[Close]

BLAST of MC03g_new0288 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-30
Identity = 69/141 (48.94%), Postives = 91/141 (64.54%), Query Frame = 0


Query: 63  HSSINHLC-KKTRLPDTNTKRWPDYEGLSFVS--DVPFVLLDMMNLRSINVDVENNIAWV 122
           H   + +C KK R+         DYEGLS+VS  D PF+L+D+  +R +N+++++N AWV
Sbjct: 95  HVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWV 154

Query: 123 QSGA-IGELYYRIAEKSKTLGFLVGLCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTII 182
           QSGA +GELYYRIAEKSK  GF  GLC ++GIGGH +GG  G M+RK+GL ADNV+   I
Sbjct: 155 QSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKI 214

Query: 183 SMTENQ-------WEDIFWAI 193
                +        ED FWAI
Sbjct: 215 VDANGKLLDRAAMGEDTFWAI 235

[Close]

BLAST of MC03g_new0288 vs. TAIR 10
Match: AT4G20830.1 (FAD-binding Berberine family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-29
Identity = 61/116 (52.59%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DY+GLS++SD PF +LDM N+R ++VD+ +N AW+ +GA +GE+YYRI EKS+  GF  G
Sbjct: 121 DYDGLSYISDKPFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAG 180

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMTENQ-------WEDIFWAI 193
           +CPTVG+GGH SGGG G M+RKFGL+ D V    I     +        ED+FWAI
Sbjct: 181 VCPTVGVGGHLSGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAI 236

[Close]

BLAST of MC03g_new0288 vs. TAIR 10
Match: AT4G20830.2 (FAD-binding Berberine family protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.2e-29
Identity = 61/116 (52.59%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 85  DYEGLSFVSDVPFVLLDMMNLRSINVDVENNIAWVQSGA-IGELYYRIAEKSKTLGFLVG 144
           DY+GLS++SD PF +LDM N+R ++VD+ +N AW+ +GA +GE+YYRI EKS+  GF  G
Sbjct: 121 DYDGLSYISDKPFFILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAG 180

Query: 145 LCPTVGIGGHFSGGGNGVMLRKFGLAADNVMSTIISMTENQ-------WEDIFWAI 193
           +CPTVG+GGH SGGG G M+RKFGL+ D V    I     +        ED+FWAI
Sbjct: 181 VCPTVGVGGHLSGGGYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAI 236

The following BLAST results are available for this feature:

BLAST of MC03g_new0288 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVG5	6.0e-31	58.62	Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=...	[more]
P93479	7.9e-31	51.09	Reticuline oxidase OS=Papaver somniferum OX=3469 GN=BBE1 PE=2 SV=1	[more]
Q9SVG3	1.8e-30	56.03	Berberine bridge enzyme-like 21 OS=Arabidopsis thaliana OX=3702 GN=At4g20840 PE=...	[more]
P30986	2.3e-30	55.65	Reticuline oxidase OS=Eschscholzia californica OX=3467 GN=BBE1 PE=1 SV=1	[more]
Q9FKU8	4.3e-29	48.94	Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=...	[more]

BLAST of MC03g_new0288 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895082.1	1.07e-44	70.69	berberine bridge enzyme-like 18 [Benincasa hispida]	[more]
OVA08657.1	6.18e-44	68.10	FAD linked oxidase [Macleaya cordata]	[more]
XP_022152520.1	7.58e-44	70.69	berberine bridge enzyme-like 18 [Momordica charantia]	[more]
XP_022994776.1	1.52e-43	63.16	berberine bridge enzyme-like 18 [Cucurbita maxima]	[more]
XP_023542351.1	2.12e-43	63.16	berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC03g_new0288 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A200QE33	2.99e-44	68.10	FAD linked oxidase OS=Macleaya cordata OX=56857 GN=BVC80_1611g5 PE=4 SV=1	[more]
A0A6J1DE62	3.67e-44	70.69	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020226 P...	[more]
A0A6J1K043	7.35e-44	63.16	berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3...	[more]
A0A6J1GTC1	1.03e-43	63.16	berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE...	[more]
A0A6J1DDW6	1.88e-43	61.87	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 P...	[more]

BLAST of MC03g_new0288 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20820.1	4.3e-32	58.62	FAD-binding Berberine family protein	[more]
AT4G20840.1	1.2e-31	56.03	FAD-binding Berberine family protein	[more]
AT5G44400.1	3.1e-30	48.94	FAD-binding Berberine family protein	[more]
AT4G20830.1	1.2e-29	52.59	FAD-binding Berberine family protein	[more]
AT4G20830.2	1.2e-29	52.59	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 85..192 e-value: 7.1E-33 score: 116.3
IPR006094	FAD linked oxidase, N-terminal	PFAM	PF01565	FAD_binding_4	coord: 97..175 e-value: 4.6E-11 score: 42.6
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 70..192
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 70..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0288.1	MC03g_new0288.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding