MC03g_new0286 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0286
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	berberine bridge enzyme-like 8
Location	MC03: 12837154 .. 12839119 (-)
RNA-Seq Expression	MC03g_new0286
Synteny	MC03g_new0286

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGGTTTTGAATTCCTCCGTTCAAAACTTCAGATTCTTGACTGCGAAGACCCCAAAACCACTCTTCATCATAACACCATCACATCTTTCCCACATTCAAATAGCTGTCATTTGTTCCAAAACTCATGGCTTTCAAATCCGAACTCGAAGCGATGGCCATGACACTGAAGGTCTTTCTTACGTTGCCTATGTTCCATTCATAATCATTGACCTAATAAATCTCAGGTCGGTCACCATTAATGTTGAACATAGTACTGCATGGGTTCAATCTGGGGCAACTCTTGGCGAGCTCTATTATAGCATCGCTCAGAAAAGTCAAACCCTTGCATTTCCAGCGGGTGTATGTCCAACAGTCGGCGTTGGTGGGCACTCCAGTGGCGGTGGATATGGGTTGATGCTGAGGAAATATGGTTTTGCAGCTGATAATGTCATCGACGCTTACTTGATTGATGCTAATGGGAAGTTTCACGATAGAGAGTCGATGGGAGAGGATTTGTTTTGGGCCATTAGAGGGGGCGGCGGAGGGAGCTTAGGGATCGTGGTGGCGTGGAAGGTGAAGCTTGTTCCGGTTCCGGCCACTGTTACGATTTGCTTGACTAACAGAAATTTGGATGAAGATTTAGTCAAGCTAATCCATCGGTGGCAATTTGTGGCTCCCAAATTGGATGAAAATTTATATGTTGGCATCATGTTGAATGGTATGTAAGATTTATGCTTCAGTTATTTATTATTTGTTTATTCGTTTTCTATGAGAAATCTACGTTTCTTCCATAAATTCTATGTCCATTCTTTTTAGGACATGAGTCATCTATTTTGACTCCATCATCCCAACATTTAGGTGTTGTTTTTATCCTTTAATTCATTTTTTTTATTTTGAAACATATTTGTTATTTTTGTGATTTGAGTTTAGATATTGTAATGACTGGATTTTCTGAGGGTTTATTTACTTGACCTCAAGTGATTTATAAGTTGATTTGTATAAGGTATTAATTCATCACCATACTTCTACCAAATAAAAACTTGCAAATCAAAAATAATGAAATCTAGTTTTGATTGTGATCTTCTAAGTCACCCCATCAAAATAAAGAAATGAGAAATAAGTTCTTTTTTCCCTAATCCCAAACATATCATTCTAAATCACTACCATTCATATTTGAATGATTGAAACTCAATTCTTGATGATAGGTGGGAATGCTTCAACTCAAGGAGGAGTCAACCCAAAATCTACATTCTTCTCATTGTTTCTTGGAAAGATAGATGAGCTTATGGCAATCTTGAAGAGAGATTTTCCAGAGATGAACTTGGCAAATAACGACTGCGTAGAAACGAGCTGGATTGAATCAACAATCATAGCCGCTACCAGGAATAAAACTGTCGAGTCCTTGGAATATTTGCTCACTAAAATACCTCTTGCCACAGGAAGCATAAAAACCAAATCTGACTACGTCAAGGAACCCATTCCCAAGGTTGCAATCGAGGGCATATGGGAAAGATTGAAAGCTCAAGATATAGAAGCGTCACAGCTTGTAATTGTTCCCTATGGAGGGAGAATGAGTCAGATTTTAGATTCGGAAATTCCTTATTCGCATAGAGCTGGATATTTGTACAAGATCGGGTATGTTGTTGGGTGGAAAGAACAGAGCGTAGATGTTGGAAAAAGGCACCTGAGTTGGATACGGGAGCTTTACGATTACATGACTCCTTTCGTTTCGAAATCACCGAGGGCTGCATATGTCAATTACAGAGACCTTGACATTGGAACAAATAACAAGTATGGAAAAACGAGCTACAAGCGAGCCAGCATTTGGGGATCAAAGTATTTTGGGAAAAATTTTAACAGGTTGGTGCACGTTAAATCTAAGGTTGATCCGTCTAATTTTTTTAGACATGAGCAAAGCATACCTCCTCTGTGACTTGGATGAGAAACGCAAAGAATCCCCCACATTTTGAAACGTGTAGAAACTAAA

mRNA sequence

ATGCTGAGGAAATATGGTTTTGCAGCTGATAATGTCATCGACGCTTACTTGATTGATGCTAATGGGAAGTTTCACGATAGAGAGTCGATGGGAGAGGATTTGTTTTGGGCCATTAGAGGGGGCGGCGGAGGGAGCTTAGGGATCGTGGTGGCGTGGAAGGTGAAGCTTGTTCCGGTTCCGGCCACTGTTACGATTTGCTTGACTAACAGAAATTTGGATGAAGATTTAGTCAAGCTAATCCATCGGTGGCAATTTGTGGCTCCCAAATTGGATGAAAATTTATATGTTGGCATCATGTTGAATGGTGGGAATGCTTCAACTCAAGGAGGAGTCAACCCAAAATCTACATTCTTCTCATTGTTTCTTGGAAAGATAGATGAGCTTATGGCAATCTTGAAGAGAGATTTTCCAGAGATGAACTTGGCAAATAACGACTGCGTAGAAACGAGCTGGATTGAATCAACAATCATAGCCGCTACCAGGAATAAAACTGTCGAGTCCTTGGAATATTTGCTCACTAAAATACCTCTTGCCACAGGAAGCATAAAAACCAAATCTGACTACGTCAAGGAACCCATTCCCAAGGTTGCAATCGAGGGCATATGGGAAAGATTGAAAGCTCAAGATATAGAAGCGTCACAGCTTGTAATTGTTCCCTATGGAGGGAGAATGAGTCAGATTTTAGATTCGGAAATTCCTTATTCGCATAGAGCTGGATATTTGTACAAGATCGGGTATGTTGTTGGGTGGAAAGAACAGAGCGTAGATGTTGGAAAAAGGCACCTGAGTTGGATACGGGAGCTTTACGATTACATGACTCCTTTCGTTTCGAAATCACCGAGGGCTGCATATGTCAATTACAGAGACCTTGACATTGGAACAAATAACAAGTATGGAAAAACGAGCTACAAGCGAGCCAGCATTTGGGGATCAAAGTATTTTGGGAAAAATTTTAACAGGTTGGTGCACGTTAAATCTAAGGTTGATCCGTCTAATTTTTTTAGACATGAGCAAAGCATACCTCCTCTGTGA

Coding sequence (CDS)

ATGCTGAGGAAATATGGTTTTGCAGCTGATAATGTCATCGACGCTTACTTGATTGATGCTAATGGGAAGTTTCACGATAGAGAGTCGATGGGAGAGGATTTGTTTTGGGCCATTAGAGGGGGCGGCGGAGGGAGCTTAGGGATCGTGGTGGCGTGGAAGGTGAAGCTTGTTCCGGTTCCGGCCACTGTTACGATTTGCTTGACTAACAGAAATTTGGATGAAGATTTAGTCAAGCTAATCCATCGGTGGCAATTTGTGGCTCCCAAATTGGATGAAAATTTATATGTTGGCATCATGTTGAATGGTGGGAATGCTTCAACTCAAGGAGGAGTCAACCCAAAATCTACATTCTTCTCATTGTTTCTTGGAAAGATAGATGAGCTTATGGCAATCTTGAAGAGAGATTTTCCAGAGATGAACTTGGCAAATAACGACTGCGTAGAAACGAGCTGGATTGAATCAACAATCATAGCCGCTACCAGGAATAAAACTGTCGAGTCCTTGGAATATTTGCTCACTAAAATACCTCTTGCCACAGGAAGCATAAAAACCAAATCTGACTACGTCAAGGAACCCATTCCCAAGGTTGCAATCGAGGGCATATGGGAAAGATTGAAAGCTCAAGATATAGAAGCGTCACAGCTTGTAATTGTTCCCTATGGAGGGAGAATGAGTCAGATTTTAGATTCGGAAATTCCTTATTCGCATAGAGCTGGATATTTGTACAAGATCGGGTATGTTGTTGGGTGGAAAGAACAGAGCGTAGATGTTGGAAAAAGGCACCTGAGTTGGATACGGGAGCTTTACGATTACATGACTCCTTTCGTTTCGAAATCACCGAGGGCTGCATATGTCAATTACAGAGACCTTGACATTGGAACAAATAACAAGTATGGAAAAACGAGCTACAAGCGAGCCAGCATTTGGGGATCAAAGTATTTTGGGAAAAATTTTAACAGGTTGGTGCACGTTAAATCTAAGGTTGATCCGTCTAATTTTTTTAGACATGAGCAAAGCATACCTCCTCTGTGA

Protein sequence

MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVPATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL

Homology

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BLAST of MC03g_new0286 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.6e-95
Identity = 172/344 (50.00%), Postives = 233/344 (67.73%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           M+RKYG  ADNV+DA ++DANGK  DR +MGED FWAIRGG GGS GI++AWK+KLVPVP
Sbjct: 198 MMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVP 257

Query: 61  ATVTICLTNRNLDEDL-VKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFS 120
            TVT+    + L +D+  K+I +WQ VA KL E L++ ++ N   A T G     +++ +
Sbjct: 258 KTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFN--VAGTGGNKTVTTSYNA 317

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           LFLG    LM ++K+ FPE+ L   DC+E SW+ES    +       +   L  K P   
Sbjct: 318 LFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPK 377

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            S K KSD+VK PIP+  ++GI+++L  +DI    ++  PYGG M++I +S+IP+ HR G
Sbjct: 378 VSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKG 437

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            L+K+ YV  W + S     RH++WIR+LY YMTP+VS +PR AYVNYRDLD+G N K  
Sbjct: 438 VLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDV 497

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 344
           KT  K+A +WG+ YF  NFNRL+ +K+KVDP NFFRHEQSIPP+
Sbjct: 498 KTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

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BLAST of MC03g_new0286 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.2e-91
Identity = 167/344 (48.55%), Postives = 236/344 (68.60%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           M+RK+G  ADNV+DA ++DANG+  DR +MGED+FWAIRGGGGGS G+++AWK+KLVPVP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKS-TFFS 120
           ATVT+    + L++D  K++++W+ +A KLD++L++ ++++  + +T+ G    S ++ +
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQA 315

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTI-IAATRNKTVESLEYLLTKIPLA 180
            FLG  + L+ ++++ FPE+ L   DC E SWI+S + IA   N      E LL    L 
Sbjct: 316 QFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAP--EALLAGKSLF 375

Query: 181 TGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVI-VPYGGRMSQILDSEIPYSHR 240
               K KSD+VKEPIP   +EG+WER   +D   S L I  PYGG MS+I +SEIP+ HR
Sbjct: 376 KNHFKAKSDFVKEPIPVEGLEGLWERFLEED---SPLTIWNPYGGMMSRISESEIPFPHR 435

Query: 241 AGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 300
            G L+KI ++  W++  V   +RH+ WIRE+Y YM  +VSK+PR AYVNYRDLD+GTN  
Sbjct: 436 NGTLFKIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE- 495

Query: 301 YGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 342
            G+T    A  WG+KY+  NF RLV +K + DP NFFRHEQS+P
Sbjct: 496 -GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

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BLAST of MC03g_new0286 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.8e-90
Identity = 167/343 (48.69%), Postives = 232/343 (67.64%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           M+RK+G  ADNV+DA ++DA+GK  +R +MGED+FWAIRGGGGGS G+++AWK+KLVPVP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFF-S 120
             VT+    R L++D  KL+++WQ VA KLDE+L++ +++   + + +      ST +  
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQG 317

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
            FLG  + L+ +++R FP++ L   DC+ETSWI+S +  A    T  S E LL    L  
Sbjct: 318 QFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPS-EALLDGKSLFK 377

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVI-VPYGGRMSQILDSEIPYSHRA 240
              K KSDYV+EPIP   +EG+WE+L  +D   S L I  PYGG M++I ++E P+ HR+
Sbjct: 378 NYFKAKSDYVEEPIPVEGLEGLWEKLLEED---SPLTIWNPYGGMMAKIPETETPFPHRS 437

Query: 241 GYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKY 300
           G L+KI ++  W++      K H+ W+RE+Y YM  +VSKSPR+AYVNYRDLD+G N   
Sbjct: 438 GTLFKIQWLTLWQDGKTSEAK-HMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN--- 497

Query: 301 GKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 342
           GK S  R   WG++YF  NF RLV +K+K DP NFFRHEQSIP
Sbjct: 498 GKGSDARE--WGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

[Close]

BLAST of MC03g_new0286 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.8e-90
Identity = 162/350 (46.29%), Postives = 231/350 (66.00%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           ++R YG AADN+IDA+L++ +GK  DR+SMGEDLFWAIRGGGG + GI+ AWK+KLV VP
Sbjct: 193 LMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVP 252

Query: 61  ATVTICLTNRNLD-EDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKST--- 120
           +  TI    +N++   LVKL ++WQ +A K D++L +       N +   G N  +    
Sbjct: 253 SKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGY 312

Query: 121 FFSLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTII---AATRNKTVESLEYLLT 180
           F S+F G +D L+ ++ + FPE+ +   DC E SWI++TI        N      E LL 
Sbjct: 313 FSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLD 372

Query: 181 KIPLATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIP 240
           +      +   K DYVK+PIP+ A+  I E+L  +D+ A   V+ PYGG M +I +S IP
Sbjct: 373 RSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIP 432

Query: 241 YSHRAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIG 300
           + HRAG +Y++ Y   W++Q  +  ++H++W+R +Y++ TP+VS++PR AY+NYRDLD+G
Sbjct: 433 FPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLG 492

Query: 301 TNNKYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 344
             N     +Y +A IWG KYFGKNFNRLV VK+KVDP+NFFR+EQSIPPL
Sbjct: 493 KTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

[Close]

BLAST of MC03g_new0286 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.0e-89
Identity = 166/347 (47.84%), Postives = 224/347 (64.55%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           ++RKYG A DNV+D  ++DANGK  DR +MGEDLFWAIRGGGG S GIV+AWK+KLVPVP
Sbjct: 201 LMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVP 260

Query: 61  ATVTICLTNRNLDEDL-VKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFS 120
            TVT+    + L++D  +K I +WQ ++ K+ E +++ ++L    A   G      T+  
Sbjct: 261 KTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRA--AGNDGNKTVTMTYLG 320

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLL-TKIPLA 180
            FLG+   L+ ++++ FPE+ L   DC E SWIE+ +       T   +E LL  K PL 
Sbjct: 321 QFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG-GFPTGSPIEILLQLKSPLG 380

Query: 181 TGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVI--VPYGGRMSQILDSEIPYSH 240
               K  SD+VKEPIP + ++GI++RL    IE +   +   PYGG MS+I +S IP+ H
Sbjct: 381 KDYFKATSDFVKEPIPVIGLKGIFKRL----IEGNTTFLNWTPYGGMMSKIPESAIPFPH 440

Query: 241 RAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNN 300
           R G L+KI Y   W E       R ++WI+E+Y+YM P+VS +PR AYVNYRDLD G N 
Sbjct: 441 RNGTLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNK 500

Query: 301 KYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 344
              K ++  A IWG KYF  NF+RLV +K+KVDP NFFRHEQSIPP+
Sbjct: 501 NNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

[Close]

BLAST of MC03g_new0286 vs. NCBI nr
Match: XP_022152519.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 701 bits (1808), Expect = 1.18e-251
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP
Sbjct: 192 MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 251

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSL 120
           ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSL
Sbjct: 252 ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSL 311

Query: 121 FLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATG 180
           FLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATG
Sbjct: 312 FLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATG 371

Query: 181 SIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGY 240
           SIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGY
Sbjct: 372 SIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGY 431

Query: 241 LYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGK 300
           LYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGK
Sbjct: 432 LYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGK 491

Query: 301 TSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 343
           TSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL
Sbjct: 492 TSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 534

[Close]

BLAST of MC03g_new0286 vs. NCBI nr
Match: XP_022152518.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 577 bits (1488), Expect = 6.19e-203
Identity = 279/346 (80.64%), Postives = 304/346 (87.86%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYG AADNVIDAYLIDANGKF+DRESMGEDLFWAIRGGGGGS GIVVAWKVKLVPVP
Sbjct: 192 MLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 251

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV---NPKSTF 120
           A VT C ++RNLDED + LIHRWQ VA KLDENLY GI+L GGNAST+GGV   NP +TF
Sbjct: 252 AIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPNPTATF 311

Query: 121 FSLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPL 180
           FSLFLGK DELMAIL  +FPE+NLA  DCVETSWIEST++AATRN+T+ESLE LL + PL
Sbjct: 312 FSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESLESLLNRTPL 371

Query: 181 ATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHR 240
              + K KSDYVKEPIPK  IEGIWERLKAQDIE  QLV VPYGGRMS+I DSE P+SHR
Sbjct: 372 TIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISDSETPFSHR 431

Query: 241 AGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 300
           A  LYKIGY+VGWKEQSV   +RHLSWIRE+YDYMTPFVSKSPRA YVNYRDLDIGTNNK
Sbjct: 432 ARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRDLDIGTNNK 491

Query: 301 YGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 343
           YGKTSYK+AS+WGSKYFG NFNRLVH+KSKVDPSNFF++EQSIPPL
Sbjct: 492 YGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

[Close]

BLAST of MC03g_new0286 vs. NCBI nr
Match: XP_022927113.1 (berberine bridge enzyme-like 28 [Cucurbita moschata])

HSP 1 Score: 524 bits (1349), Expect = 6.70e-184
Identity = 246/342 (71.93%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYG AADNV+DAYL+DANG+ HDRESMGEDLFWAIRGGGGGS GIVVAWKVKLV VP
Sbjct: 55  MLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVVVP 114

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV-NPKSTFFS 120
           A VT+C TNR++++D +KLIHRWQ+VA +LD+NL++GI+L GGN ST+ G+ NP +TFFS
Sbjct: 115 AVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNPTATFFS 174

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           L+LGK+DEL+AILK  FPE+ L   DCVE+SWI+ST+IA+T  +T E LE LL + P   
Sbjct: 175 LYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTI 234

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            S K KSDYVK+PIP+ AIEGIW+RLKAQDIE  Q++ +PYGGRMSQI +SE P+SHRAG
Sbjct: 235 ESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAG 294

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            LYKIGY V WKEQS+D   RH++WIRE+Y+Y+TPFVSKSPRAAYVNYRDLDIGTNNKYG
Sbjct: 295 NLYKIGYAVSWKEQSLDAKNRHINWIREVYEYITPFVSKSPRAAYVNYRDLDIGTNNKYG 354

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 341
           KTSY++ASIWG KYFG NFNRLV+VK+ VD  +FFRHEQSIP
Sbjct: 355 KTSYEQASIWGMKYFGNNFNRLVYVKTMVDSHDFFRHEQSIP 396

[Close]

BLAST of MC03g_new0286 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 527 bits (1358), Expect = 3.32e-183
Identity = 250/344 (72.67%), Postives = 291/344 (84.59%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRK+G AADNVIDA+L+DANGK HDRESMGEDLFWAIRGGGGGS GIVVAWK+KLVPVP
Sbjct: 193 MLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVAWKIKLVPVP 252

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV-NPKSTFFS 120
           ATVTIC T RNL+ED +KL+HRWQ+VA KLDENLY+GI+L GG ASTQGG+ NP +TFFS
Sbjct: 253 ATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQGGITNPTATFFS 312

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           LFLG+IDEL+A L   FPE+ L   DCVE +W+EST+I AT  + +ESLE LL + P   
Sbjct: 313 LFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIESLEPLLNRTPPTI 372

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            + K KSDYVKEP+P+ AIEGI +RLKAQDIE SQL+ VPYGG+MSQI +SE P+SHRAG
Sbjct: 373 ENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQISESETPFSHRAG 432

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            LYKIGYVVGWKEQS+   KRH+ WIRE+Y+YM PFVSKSPRAAY NYRDLDIG NNKYG
Sbjct: 433 NLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANYRDLDIGANNKYG 492

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 343
           +TS ++AS+WG KYFG NF +LV+VK+KVDP +FFRHEQSIP L
Sbjct: 493 QTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

[Close]

BLAST of MC03g_new0286 vs. NCBI nr
Match: XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525 bits (1352), Expect = 2.61e-182
Identity = 246/342 (71.93%), Postives = 294/342 (85.96%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYG AADNV+DAYL+DANG+ HDRESMGEDLFWAIRGGGGGS GIVVAWKVKLV VP
Sbjct: 190 MLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVVVP 249

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV-NPKSTFFS 120
           A VT+C TNR++++D +KLIHRWQ+VA +LD+NL++GI+L GGN ST+ G+ NP +TFFS
Sbjct: 250 AVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGITNPTATFFS 309

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           L+LGK+DEL+AILK  FPE+ L   DCVE+SWI+ST+IA+T  +T E LE LL + P   
Sbjct: 310 LYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTI 369

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            S K KSDYVK+PIP+ AIEGIW+RLKAQDIE  Q++ +PYGGRMSQI +SE P+SHRAG
Sbjct: 370 ESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAG 429

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            LYKIGY V WKEQS+D   RH++WIRE+ ++MTPFVSKSPRAAYVNYRDLDIGTNNKYG
Sbjct: 430 NLYKIGYAVSWKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDLDIGTNNKYG 489

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 341
           KTSY++AS+WG KYFG NFNRLV+VK+KVDP +FFRHEQSIP
Sbjct: 490 KTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

[Close]

BLAST of MC03g_new0286 vs. ExPASy TrEMBL
Match: A0A6J1DG89 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111020225 PE=3 SV=1)

HSP 1 Score: 701 bits (1808), Expect = 5.73e-252
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP
Sbjct: 192 MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 251

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSL 120
           ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSL
Sbjct: 252 ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFSL 311

Query: 121 FLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATG 180
           FLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATG
Sbjct: 312 FLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLATG 371

Query: 181 SIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGY 240
           SIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGY
Sbjct: 372 SIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAGY 431

Query: 241 LYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGK 300
           LYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGK
Sbjct: 432 LYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGK 491

Query: 301 TSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 343
           TSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL
Sbjct: 492 TSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 534

[Close]

BLAST of MC03g_new0286 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 577 bits (1488), Expect = 3.00e-203
Identity = 279/346 (80.64%), Postives = 304/346 (87.86%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYG AADNVIDAYLIDANGKF+DRESMGEDLFWAIRGGGGGS GIVVAWKVKLVPVP
Sbjct: 192 MLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 251

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV---NPKSTF 120
           A VT C ++RNLDED + LIHRWQ VA KLDENLY GI+L GGNAST+GGV   NP +TF
Sbjct: 252 AIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEGGVKKPNPTATF 311

Query: 121 FSLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPL 180
           FSLFLGK DELMAIL  +FPE+NLA  DCVETSWIEST++AATRN+T+ESLE LL + PL
Sbjct: 312 FSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIESLESLLNRTPL 371

Query: 181 ATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHR 240
              + K KSDYVKEPIPK  IEGIWERLKAQDIE  QLV VPYGGRMS+I DSE P+SHR
Sbjct: 372 TIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSRISDSETPFSHR 431

Query: 241 AGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 300
           A  LYKIGY+VGWKEQSV   +RHLSWIRE+YDYMTPFVSKSPRA YVNYRDLDIGTNNK
Sbjct: 432 ARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVNYRDLDIGTNNK 491

Query: 301 YGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 343
           YGKTSYK+AS+WGSKYFG NFNRLVH+KSKVDPSNFF++EQSIPPL
Sbjct: 492 YGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

[Close]

BLAST of MC03g_new0286 vs. ExPASy TrEMBL
Match: A0A6J1EK38 (berberine bridge enzyme-like 28 OS=Cucurbita moschata OX=3662 GN=LOC111434051 PE=3 SV=1)

HSP 1 Score: 524 bits (1349), Expect = 3.24e-184
Identity = 246/342 (71.93%), Postives = 293/342 (85.67%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYG AADNV+DAYL+DANG+ HDRESMGEDLFWAIRGGGGGS GIVVAWKVKLV VP
Sbjct: 55  MLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVVVP 114

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV-NPKSTFFS 120
           A VT+C TNR++++D +KLIHRWQ+VA +LD+NL++GI+L GGN ST+ G+ NP +TFFS
Sbjct: 115 AVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNPTATFFS 174

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           L+LGK+DEL+AILK  FPE+ L   DCVE+SWI+ST+IA+T  +T E LE LL + P   
Sbjct: 175 LYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTI 234

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            S K KSDYVK+PIP+ AIEGIW+RLKAQDIE  Q++ +PYGGRMSQI +SE P+SHRAG
Sbjct: 235 ESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAG 294

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            LYKIGY V WKEQS+D   RH++WIRE+Y+Y+TPFVSKSPRAAYVNYRDLDIGTNNKYG
Sbjct: 295 NLYKIGYAVSWKEQSLDAKNRHINWIREVYEYITPFVSKSPRAAYVNYRDLDIGTNNKYG 354

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 341
           KTSY++ASIWG KYFG NFNRLV+VK+ VD  +FFRHEQSIP
Sbjct: 355 KTSYEQASIWGMKYFGNNFNRLVYVKTMVDSHDFFRHEQSIP 396

[Close]

BLAST of MC03g_new0286 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 523 bits (1348), Expect = 6.49e-182
Identity = 252/345 (73.04%), Postives = 290/345 (84.06%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRK+G AADNVIDAYL+DANGKF DRE MGEDLFWAIRGGGGGS GIVVAWKVKLVPVP
Sbjct: 196 MLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 255

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGG--VNPKSTFF 120
           ATVT C  NR L+E  +KLIHRWQ+VA KLDENL++GI+L GGN + QGG   NP ++FF
Sbjct: 256 ATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQGGDKTNPTASFF 315

Query: 121 SLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLA 180
           SLFLGK DE  AIL   FPE+ LA  +C+E+SWIES +++A   K  +SLE LL + PL 
Sbjct: 316 SLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGDSLEALLNRTPLT 375

Query: 181 TGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRA 240
            GSIK KSDYVKEPI +  IEGIWERLK++D+ A QL+ VPYGGRMSQI +SEIP+SHRA
Sbjct: 376 NGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGGRMSQISESEIPFSHRA 435

Query: 241 GYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKY 300
           G LYKIGY+V W++Q+VDV KRHL WIRELY YMTPFVSKSPR+AYVNYRDLDIG+NNKY
Sbjct: 436 GNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRSAYVNYRDLDIGSNNKY 495

Query: 301 GKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 343
           GKTS+KRAS+WG KYFG NFNRLVHVK+KVDP +FFRHEQSIP +
Sbjct: 496 GKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTI 539

[Close]

BLAST of MC03g_new0286 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 1.03e-181
Identity = 243/342 (71.05%), Postives = 292/342 (85.38%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           MLRKYG AADNV+DAYL+DANG+ HDRESMGEDLFWAIRGGGGGS GIVVAWKVKLV VP
Sbjct: 190 MLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVMVP 249

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGV-NPKSTFFS 120
           A VT+C TNR++++D +KLIHRWQ+VA +LD+NL++GI+L GGN ST+ G+ NP +TFFS
Sbjct: 250 AVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNPTATFFS 309

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           L+LGK+DEL+ ILK +F E+ L   DCVE+SWI+ST+IA+T  +T E LE LL + P   
Sbjct: 310 LYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLNRTPPTI 369

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            S K KSDYVK+PIP+  IEGIW+RLKAQDIE  Q++ +PYGGRMSQI +SE P+SHRAG
Sbjct: 370 ESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETPFSHRAG 429

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            LYKIGY V WKEQS+D   +H++WIRE+Y+YMTPFVSKSPRAAY NYRDLDIGTNNKYG
Sbjct: 430 NLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDIGTNNKYG 489

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 341
           KTSY++AS+WG KYFG NFNRLV+VK+KVDP +FFRHEQSIP
Sbjct: 490 KTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

[Close]

BLAST of MC03g_new0286 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.2e-96
Identity = 172/344 (50.00%), Postives = 233/344 (67.73%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           M+RKYG  ADNV+DA ++DANGK  DR +MGED FWAIRGG GGS GI++AWK+KLVPVP
Sbjct: 198 MMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVP 257

Query: 61  ATVTICLTNRNLDEDL-VKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFS 120
            TVT+    + L +D+  K+I +WQ VA KL E L++ ++ N   A T G     +++ +
Sbjct: 258 KTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFN--VAGTGGNKTVTTSYNA 317

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
           LFLG    LM ++K+ FPE+ L   DC+E SW+ES    +       +   L  K P   
Sbjct: 318 LFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPK 377

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSHRAG 240
            S K KSD+VK PIP+  ++GI+++L  +DI    ++  PYGG M++I +S+IP+ HR G
Sbjct: 378 VSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKG 437

Query: 241 YLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYG 300
            L+K+ YV  W + S     RH++WIR+LY YMTP+VS +PR AYVNYRDLD+G N K  
Sbjct: 438 VLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDV 497

Query: 301 KTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 344
           KT  K+A +WG+ YF  NFNRL+ +K+KVDP NFFRHEQSIPP+
Sbjct: 498 KTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

[Close]

BLAST of MC03g_new0286 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.3e-92
Identity = 167/344 (48.55%), Postives = 236/344 (68.60%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           M+RK+G  ADNV+DA ++DANG+  DR +MGED+FWAIRGGGGGS G+++AWK+KLVPVP
Sbjct: 196 MMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 255

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKS-TFFS 120
           ATVT+    + L++D  K++++W+ +A KLD++L++ ++++  + +T+ G    S ++ +
Sbjct: 256 ATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTISMSYQA 315

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTI-IAATRNKTVESLEYLLTKIPLA 180
            FLG  + L+ ++++ FPE+ L   DC E SWI+S + IA   N      E LL    L 
Sbjct: 316 QFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAP--EALLAGKSLF 375

Query: 181 TGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVI-VPYGGRMSQILDSEIPYSHR 240
               K KSD+VKEPIP   +EG+WER   +D   S L I  PYGG MS+I +SEIP+ HR
Sbjct: 376 KNHFKAKSDFVKEPIPVEGLEGLWERFLEED---SPLTIWNPYGGMMSRISESEIPFPHR 435

Query: 241 AGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNK 300
            G L+KI ++  W++  V   +RH+ WIRE+Y YM  +VSK+PR AYVNYRDLD+GTN  
Sbjct: 436 NGTLFKIQWLSTWQDGKVS-EERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTNE- 495

Query: 301 YGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 342
            G+T    A  WG+KY+  NF RLV +K + DP NFFRHEQS+P
Sbjct: 496 -GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

[Close]

BLAST of MC03g_new0286 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 332.0 bits (850), Expect = 5.6e-91
Identity = 167/343 (48.69%), Postives = 232/343 (67.64%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           M+RK+G  ADNV+DA ++DA+GK  +R +MGED+FWAIRGGGGGS G+++AWK+KLVPVP
Sbjct: 198 MMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVP 257

Query: 61  ATVTICLTNRNLDEDLVKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFF-S 120
             VT+    R L++D  KL+++WQ VA KLDE+L++ +++   + + +      ST +  
Sbjct: 258 EIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTISTSYQG 317

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLLTKIPLAT 180
            FLG  + L+ +++R FP++ L   DC+ETSWI+S +  A    T  S E LL    L  
Sbjct: 318 QFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPS-EALLDGKSLFK 377

Query: 181 GSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVI-VPYGGRMSQILDSEIPYSHRA 240
              K KSDYV+EPIP   +EG+WE+L  +D   S L I  PYGG M++I ++E P+ HR+
Sbjct: 378 NYFKAKSDYVEEPIPVEGLEGLWEKLLEED---SPLTIWNPYGGMMAKIPETETPFPHRS 437

Query: 241 GYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNNKY 300
           G L+KI ++  W++      K H+ W+RE+Y YM  +VSKSPR+AYVNYRDLD+G N   
Sbjct: 438 GTLFKIQWLTLWQDGKTSEAK-HMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN--- 497

Query: 301 GKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIP 342
           GK S  R   WG++YF  NF RLV +K+K DP NFFRHEQSIP
Sbjct: 498 GKGSDARE--WGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

[Close]

BLAST of MC03g_new0286 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.3e-91
Identity = 166/347 (47.84%), Postives = 224/347 (64.55%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           ++RKYG A DNV+D  ++DANGK  DR +MGEDLFWAIRGGGG S GIV+AWK+KLVPVP
Sbjct: 201 LMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVP 260

Query: 61  ATVTICLTNRNLDEDL-VKLIHRWQFVAPKLDENLYVGIMLNGGNASTQGGVNPKSTFFS 120
            TVT+    + L++D  +K I +WQ ++ K+ E +++ ++L    A   G      T+  
Sbjct: 261 KTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRA--AGNDGNKTVTMTYLG 320

Query: 121 LFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTIIAATRNKTVESLEYLL-TKIPLA 180
            FLG+   L+ ++++ FPE+ L   DC E SWIE+ +       T   +E LL  K PL 
Sbjct: 321 QFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHG-GFPTGSPIEILLQLKSPLG 380

Query: 181 TGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVI--VPYGGRMSQILDSEIPYSH 240
               K  SD+VKEPIP + ++GI++RL    IE +   +   PYGG MS+I +S IP+ H
Sbjct: 381 KDYFKATSDFVKEPIPVIGLKGIFKRL----IEGNTTFLNWTPYGGMMSKIPESAIPFPH 440

Query: 241 RAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNN 300
           R G L+KI Y   W E       R ++WI+E+Y+YM P+VS +PR AYVNYRDLD G N 
Sbjct: 441 RNGTLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNK 500

Query: 301 KYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 344
              K ++  A IWG KYF  NF+RLV +K+KVDP NFFRHEQSIPP+
Sbjct: 501 NNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

[Close]

BLAST of MC03g_new0286 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 329.7 bits (844), Expect = 2.8e-90
Identity = 165/347 (47.55%), Postives = 226/347 (65.13%), Query Frame = 0


Query: 1   MLRKYGFAADNVIDAYLIDANGKFHDRESMGEDLFWAIRGGGGGSLGIVVAWKVKLVPVP 60
           ++RKYG AADNV+DA ++DANGK  DR SMGEDLFWAIRGG GGS GI+++WK+KLVPVP
Sbjct: 200 LMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVP 259

Query: 61  ATVTICLTNRNLDED-LVKLIHRWQFVAPKLDENLYVGIMLN-GGNASTQGGVNPKSTFF 120
            T+T+    +  ++D   K++ +WQ +A  L + L++ +     GN + +        + 
Sbjct: 260 ETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGNKANK---TVTMAYI 319

Query: 121 SLFLGKIDELMAILKRDFPEMNLANNDCVETSWIESTII-AATRNKTVESLEYLL-TKIP 180
             FLG+   LM ++K+DFPE+ L   DC+E SWI+S I  +         +E LL  K P
Sbjct: 320 GQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKSP 379

Query: 181 LATGSIKTKSDYVKEPIPKVAIEGIWERLKAQDIEASQLVIVPYGGRMSQILDSEIPYSH 240
           +     K KSD+ K+PIP + +EG++++L  +D  A+ ++  PYGG+M +I +SEIP+ H
Sbjct: 380 IGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEED--AALVIWTPYGGKMDKIPESEIPFPH 439

Query: 241 RAGYLYKIGYVVGWKEQSVDVGKRHLSWIRELYDYMTPFVSKSPRAAYVNYRDLDIGTNN 300
           R G  + I Y   W + S     R   WIRELY YMTP+VS +PR AYVNYRDLD+G N 
Sbjct: 440 RNGTNFMIQYYRSWSD-SEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQNK 499

Query: 301 KYGKTSYKRASIWGSKYFGKNFNRLVHVKSKVDPSNFFRHEQSIPPL 344
              K+++  A IWG+ YF  NFNRLV +KSKVDP NFFRHEQSIP L
Sbjct: 500 DNSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

The following BLAST results are available for this feature:

BLAST of MC03g_new0286 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKU8	1.6e-95	50.00	Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=...	[more]
O64743	3.2e-91	48.55	Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV...	[more]
Q93ZA3	7.8e-90	48.69	Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=...	[more]
Q8GTB6	7.8e-90	46.29	Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV...	[more]
Q9FKV0	1.0e-89	47.84	Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=...	[more]

BLAST of MC03g_new0286 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152519.1	1.18e-251	100.00	tetrahydrocannabinolic acid synthase-like [Momordica charantia]	[more]
XP_022152518.1	6.19e-203	80.64	berberine bridge enzyme-like 8 [Momordica charantia]	[more]
XP_022927113.1	6.70e-184	71.93	berberine bridge enzyme-like 28 [Cucurbita moschata]	[more]
XP_038895543.1	3.32e-183	72.67	berberine bridge enzyme-like 18 [Benincasa hispida]	[more]
XP_023520318.1	2.61e-182	71.93	berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC03g_new0286 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DG89	5.73e-252	100.00	tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1DF27	3.00e-203	80.64	berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE...	[more]
A0A6J1EK38	3.24e-184	71.93	berberine bridge enzyme-like 28 OS=Cucurbita moschata OX=3662 GN=LOC111434051 PE...	[more]
A0A6J1DHZ2	6.49e-182	73.04	berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 P...	[more]
A0A6J1KHF6	1.03e-181	71.05	berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3...	[more]

BLAST of MC03g_new0286 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44400.1	1.2e-96	50.00	FAD-binding Berberine family protein	[more]
AT2G34790.1	2.3e-92	48.55	FAD-binding Berberine family protein	[more]
AT1G30760.1	5.6e-91	48.69	FAD-binding Berberine family protein	[more]
AT5G44380.1	7.3e-91	47.84	FAD-binding Berberine family protein	[more]
AT5G44390.1	2.8e-90	47.55	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012951	Berberine/berberine-like	PFAM	PF08031	BBE	coord: 283..341 e-value: 1.7E-22 score: 79.3
None	No IPR available	GENE3D	3.40.462.20		coord: 62..279 e-value: 1.6E-123 score: 414.8
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 1..343
None	No IPR available	PANTHER	PTHR32448:SF113	BERBERINE BRIDGE ENZYME-LIKE 18	coord: 1..343
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 1..342 e-value: 1.6E-123 score: 414.8
IPR016166	FAD-binding domain, PCMH-type	PROSITE	PS51387	FAD_PCMH	coord: 1..59 score: 9.259071
IPR036318	FAD-binding, type PCMH-like superfamily	SUPERFAMILY	56176	FAD-binding/transporter-associated domain-like	coord: 4..60

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0286.1	MC03g_new0286.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding