Homology
BLAST of MC03g_new0248 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 855.9 bits (2210), Expect = 3.5e-247
Identity = 441/760 (58.03%), Postives = 535/760 (70.39%), Query Frame = 0
Query: 11 SLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYS 70
+L +P+FA KK Y+V LGSH+H +IS L VA+SH L S +GS + AK+AIFYS
Sbjct: 29 TLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYS 88
Query: 71 YKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPSSAW 130
YK+HINGFAA+L+E +AA +AKHP+V V ++ KLHTTHSW FM L + SS W
Sbjct: 89 YKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLW 148
Query: 131 GKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNRKLIGAKFYNE 190
KA +GEDTIIANLD+GVWPES SF D G +P+RWKG C + PCNRKLIGA+++N+
Sbjct: 149 NKAGYGEDTIIANLDTGVWPESKSFSDEGY-GAVPARWKGRCHKDVPCNRKLIGARYFNK 208
Query: 191 GYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKGGSP 250
GY+AY + N + T RD GHGSHTLSTA GNFV NVFG G G A GGSP
Sbjct: 209 GYLAY-TGLPSNASYET----CRDHDGHGSHTLSTAAGNFVPGANVFG-IGNGTASGGSP 268
Query: 251 KARLAAYKVCWGSNVG--CPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDFFAIASF 310
KAR+AAYKVCW G C ADIL ++ AI DGVDV+S S+GG Y D AI SF
Sbjct: 269 KARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSF 328
Query: 311 QAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSLS 370
AVKNG++VVCSAGN+GP GT N+APW+ TVGAS++DR A V+L NGQ F+G+SLS
Sbjct: 329 HAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS 388
Query: 371 KGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGTE 430
K LPEEK Y LIS A + DA+LCK G+LDP KVKGKIL C+RG+ AR+DKG +
Sbjct: 389 KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQ 448
Query: 431 AAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPSE 490
AA AGA GM+LCND+ G SD HVLPA+ I Y+ +F YL +T++P Y+ P+
Sbjct: 449 AAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTA 508
Query: 491 KFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPYM 550
+ KPAP MA FSSRGPN ITP I+KPDITAPGVNIIAA+T++ GP++ SD RR P+
Sbjct: 509 TLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFN 568
Query: 551 TMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDPA 610
T SGTSMSCPHISGVVGLLKTLHP WSPAAI+SAIMTT+R R+N PM+D + A
Sbjct: 569 TESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKK---A 628
Query: 611 TPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSASL 670
PFSYGSGHVQP A PGLVYDLTT DYL+FLCA+GY+ ++++ F++D Y C A+L
Sbjct: 629 NPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANL 688
Query: 671 LDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGEEK 730
LDFNYPSI V NL G +TVTRKLKNVG P Y A R P GV V+VEP+ L F GE K
Sbjct: 689 LDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVK 748
Query: 731 SFSVTLAG-KLPQNAVVDGTLIWSDGKHFVRSPIVVSSGS 768
F +TL + + V G L W+D H+VRSPIVV S
Sbjct: 749 IFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLSS 778
BLAST of MC03g_new0248 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 748.8 bits (1932), Expect = 6.0e-215
Identity = 407/776 (52.45%), Postives = 518/776 (66.75%), Query Frame = 0
Query: 5 FSFFVFSLLLTPS----FAAK--KPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLG 64
FSF + LL+ S A+K YVV G+HSH EI+E + RV +HY LGS G
Sbjct: 7 FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66
Query: 65 STKKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHL 124
S ++A DAIFYSY KHINGFAA L+ + A ++KHPEV V ++ +KLHTT SW F+ L
Sbjct: 67 SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126
Query: 125 ERKNSSIPSSA-WGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQ--- 184
E NS +PSS+ W KARFGEDTIIANLD+GVWPES SFRD G+ PIPSRWKG C+
Sbjct: 127 EH-NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKD 186
Query: 185 -GFPCNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAA 244
F CNRKLIGA+++N+GY A + ++N +F +S RD GHGSHTLSTA G+FV
Sbjct: 187 ATFHCNRKLIGARYFNKGYAAAVGHLNSSF------DSPRDLDGHGSHTLSTAAGDFVPG 246
Query: 245 KNVFGSSGFGMAKGGSPKARLAAYKVCWGSNVG--CPGADILQGLDDAIHDGVDVVSYSI 304
++FG G G AKGGSP+AR+AAYKVCW G C AD+L D AIHDG DV+S S+
Sbjct: 247 VSIFG-QGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSL 306
Query: 305 GGVNVPYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIV 364
GG ++ D AI SF A K I VVCSAGN+GP T N+APW TVGAST+DR
Sbjct: 307 GGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFA 366
Query: 365 AAVQLNNGQRFQGSSLSK-GLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKG 424
+ + L NG+ ++G SLS LP K+YP+++ A A A DA LCK G+LDP K KG
Sbjct: 367 SNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKG 426
Query: 425 KILACMRGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVF 484
KIL C+RG+ R++KG A G +GM+L N G +D HVLPAT + + S AV
Sbjct: 427 KILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVS 486
Query: 485 DYLDNTQNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYT 544
Y+ T+ P+A++ P LKPAP MA FSS+GP+++ P+I+KPDITAPGV++IAAYT
Sbjct: 487 RYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYT 546
Query: 545 QSHGPSEDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVR 604
+ P+ + D RR+ + +SGTSMSCPHISG+ GLLKT +P WSPAAI+SAIMTTA +
Sbjct: 547 GAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIM 606
Query: 605 DNTMSPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSM 664
D+ P+ + + + ATPFS+G+GHVQP A++PGLVYDL +DYLNFLC+LGY+ S
Sbjct: 607 DDIPGPIQNATNMK---ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQ 666
Query: 665 IRAFSDDTPYDCPPSASLLDFNYPSIGVQNL-NGQVTVTRKLKNVGSPGVYRASVRRPEG 724
I FS + P SL++ NYPSI V NL + +VTV+R +KNVG P +Y V P+G
Sbjct: 667 ISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQG 726
Query: 725 VAVTVEPRVLRFENVGEEKSFSVTLAGKLPQNA--VVDGTLIWSDGKHFVRSPIVV 764
V V V+P L F VGE+K+F V L A V G L+WSD KH VRSPIVV
Sbjct: 727 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVV 770
BLAST of MC03g_new0248 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 645.2 bits (1663), Expect = 9.4e-184
Identity = 371/790 (46.96%), Postives = 480/790 (60.76%), Query Frame = 0
Query: 3 VLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKK 62
+L SFF+F+ LL +KK Y+V +G+HSHG + DL+ +SHY LLGS+ GS +K
Sbjct: 11 ILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREK 70
Query: 63 AKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKN 122
AK+AI YSY +HINGFAA+LEEE+AA +AK+P V V + KLHTT SW F+ L R+
Sbjct: 71 AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG 130
Query: 123 SSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGS-CE-------Q 182
+SAW K RFGE+TII N+D+GVWPES SF D G +PS+W+G C+
Sbjct: 131 Q---NSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGY-GTVPSKWRGGLCQINKLPGSM 190
Query: 183 GFPCNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAK 242
CNRKLIGA++YN+ + A+ ++P + ++ARD GHG+HTLSTAGGNFV
Sbjct: 191 KNTCNRKLIGARYYNKAFEAHNGQLDP------LLHTARDFVGHGTHTLSTAGGNFVPGA 250
Query: 243 NVFGSSGFGMAKGGSPKARLAAYKVCWG--SNVGCPGADILQGLDDAIHDGVDVVSYSIG 302
VF + G G AKGGSP+AR+AAYKVCW C GAD+L +D AI DGVDV++ S G
Sbjct: 251 RVF-AVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFG 310
Query: 303 GVNV----PYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDR 362
V + D +I +F A+ I +V SAGN GP PGT N+APW+FT+ ASTLDR
Sbjct: 311 VSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDR 370
Query: 363 RIVAAVQLNNGQRFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKV 422
+ + +NN Q +G+SL LP + + LI A A DA LC+ G LD KV
Sbjct: 371 DFSSNLTINN-QLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKV 430
Query: 423 KGKILACMR-GETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQ--- 482
GKI+ C R G+ + +G EA AGA GMIL N ++G+ ++ HV + +
Sbjct: 431 NGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAK 490
Query: 483 ------ASLAVFDYLDNTQNPMAYLVPPSEK-FDLKPAPTMADFSSRGPNVITPEIIKPD 542
+ A+ D D + + + F KPAP MA FSSRGPN I P I+KPD
Sbjct: 491 SRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPD 550
Query: 543 ITAPGVNIIAAYTQSHGPSEDPSDGRR-VPYMTMSGTSMSCPHISGVVGLLKTLHPDWSP 602
+TAPGVNI+AAY++ S D RR + + GTSMSCPH SG+ GLLKT HP WSP
Sbjct: 551 VTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSP 610
Query: 603 AAIKSAIMTTARVRDNTMSPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTED 662
AAIKSAIMTTA DNT P+ D A F+YGSGHV+P A++PGLVYDL+ D
Sbjct: 611 AAIKSAIMTTATTLDNTNRPIQDAFDK--TLADAFAYGSGHVRPDLAIEPGLVYDLSLTD 670
Query: 663 YLNFLCALGYDWSMIRAFSDDTPYDCPPSASLLDFNYPSIGVQNLN-GQVTVTRKLKNVG 722
YLNFLCA GYD +I A + + + C S S+ D NYPSI + NL VT+ R + NVG
Sbjct: 671 YLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVG 730
Query: 723 SPGVYRASVRRPEGVAVTVEPRVLRFENVGEEKSFSVTL--AGKLPQNAVVDGTLIWSDG 764
P Y S R P G ++ V P L F +GE K+F V + + + G L W+DG
Sbjct: 731 PPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDG 786
BLAST of MC03g_new0248 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 558.5 bits (1438), Expect = 1.2e-157
Identity = 339/791 (42.86%), Postives = 451/791 (57.02%), Query Frame = 0
Query: 1 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 60
MA F FF L +PS +A S ++ + + + +H+ S L S
Sbjct: 1 MAFFFYFFFLLTLSSPSSSASSS-----NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL 60
Query: 61 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 120
+ +I ++Y +GF+A L +DA+ L HP V V+ +++ LHTT S F+ L
Sbjct: 61 TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGL-- 120
Query: 121 KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSC--EQGFP- 180
S+ + ++ FG D +I +D+GVWPE SF D G+ P+P +WKG C Q FP
Sbjct: 121 -RSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGL-GPVPIKWKGQCIASQDFPE 180
Query: 181 --CNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKN 240
CNRKL+GA+F+ GY A N N +T S RD GHG+HT S + G +V +
Sbjct: 181 SACNRKLVGARFFCGGYEA----TNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPAS 240
Query: 241 VFGSSGFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVN 300
G + G+A G +PKARLAAYKVCW N GC +DIL D A+ DGVDV+S S+GGV
Sbjct: 241 TLGYA-HGVAAGMAPKARLAAYKVCW--NSGCYDSDILAAFDTAVADGVDVISLSVGGVV 300
Query: 301 VPYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQ 360
VPYY D AI +F A+ GI V SAGN GPG T N+APW+ TVGA T+DR A V+
Sbjct: 301 VPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVK 360
Query: 361 LNNGQRFQGSSL--SKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKIL 420
L NG+ G S+ GL + YPL+ GG G Y + LC G+LDP VKGKI+
Sbjct: 361 LGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY--SSSLCLEGSLDPNLVKGKIV 420
Query: 421 ACMRGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYL 480
C RG +R KG K G +GMI+ N DGEG +D HVLPAT +G + Y+
Sbjct: 421 LCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYI 480
Query: 481 D------NTQNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIA 540
++++P A +V + ++PAP +A FS+RGPN TPEI+KPD+ APG+NI+A
Sbjct: 481 SESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILA 540
Query: 541 AYTQSHGPSEDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTA 600
A+ GPS SD RR + +SGTSM+CPH+SG+ LLK HPDWSPAAI+SA++TTA
Sbjct: 541 AWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTA 600
Query: 601 RVRDNTMSPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYD 660
DN+ PM+D + G+ ++ YGSGHV P AMDPGLVYD+T+ DY+NFLC Y
Sbjct: 601 YTVDNSGEPMMDEST--GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYT 660
Query: 661 WSMIRAFSDDTPYDCP---PSASLLDFNYPSIGV--QNLNGQVTVT---RKLKNVG-SPG 720
+ I + DC + + + NYPS V Q T R + NVG S
Sbjct: 661 RTNIVTIT-RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDS 720
Query: 721 VYRASVRRPEGVAVTVEPRVLRFENVGEEKSFSV---TLAGKLPQNA--VVDGTLIWSDG 765
VY +R P G VTVEP L F VG++ SF V T KL A V G ++WSDG
Sbjct: 721 VYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDG 770
BLAST of MC03g_new0248 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 557.8 bits (1436), Expect = 2.0e-157
Identity = 326/712 (45.79%), Postives = 430/712 (60.39%), Query Frame = 0
Query: 67 IFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIP 126
+ Y+Y+ I+GF+ L +E+A +L P V VL + +LHTT + F+ L+ + +
Sbjct: 65 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL- 124
Query: 127 SSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFP-----CNRK 186
+ +A D ++ LD+GVWPES S+ D G PIPS WKG CE G CNRK
Sbjct: 125 ---FPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLCNRK 184
Query: 187 LIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSG 246
LIGA+F+ GY + M P + S + S RDD GHG+HT STA G+ V ++ G +
Sbjct: 185 LIGARFFARGYES---TMGP-IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 244
Query: 247 FGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQD 306
G A+G +P+AR+A YKVCW GC +DIL +D AI D V+V+S S+GG YY+D
Sbjct: 245 -GTARGMAPRARVAVYKVCWLG--GCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 304
Query: 307 FFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQR 366
AI +F A++ GI V CSAGNAGP + N+APW+ TVGA TLDR A L NG+
Sbjct: 305 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 364
Query: 367 FQGSSLSKG--LPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGE 426
F G SL KG LP +K P I G A+ + LC G L P KVKGKI+ C RG
Sbjct: 365 FTGVSLFKGEALP-DKLLPFIYAGNASNA----TNGNLCMTGTLIPEKVKGKIVMCDRGI 424
Query: 427 TARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNP 486
AR+ KG AG VGMIL N +GE +D H+LPAT +G +A + Y+ NP
Sbjct: 425 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 484
Query: 487 MAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDP 546
A + +KP+P +A FSSRGPN ITP I+KPD+ APGVNI+AA+T + GP+
Sbjct: 485 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 544
Query: 547 SDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMID 606
SD RRV + +SGTSMSCPH+SG+ LLK++HP+WSPAAI+SA+MTTA P++D
Sbjct: 545 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 604
Query: 607 GGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTP 666
+ G P+TPF +G+GHV P A +PGL+YDLTTEDYL FLCAL Y IR+ S
Sbjct: 605 IAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN- 664
Query: 667 YDCPPSA--SLLDFNYPSIGVQNLN--GQVTVTRKLKNVGSPGVYRASV-RRPEGVAVTV 726
Y C PS S+ D NYPS V N++ G TR + +VG G Y V GV ++V
Sbjct: 665 YTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISV 724
Query: 727 EPRVLRFENVGEEKSFSVTLA--GKLPQNAVVDGTLIWSDGKHFVRSPIVVS 765
EP VL F+ E+KS++VT P + G++ WSDGKH V SP+ +S
Sbjct: 725 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of MC03g_new0248 vs. NCBI nr
Match:
XP_022142619.1 (subtilisin-like protease SBT5.4 [Momordica charantia])
HSP 1 Score: 1540 bits (3986), Expect = 0.0
Identity = 768/769 (99.87%), Postives = 768/769 (99.87%), Query Frame = 0
Query: 1 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 60
MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST
Sbjct: 9 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 68
Query: 61 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 120
KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER
Sbjct: 69 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 128
Query: 121 KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNR 180
KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNR
Sbjct: 129 KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNR 188
Query: 181 KLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSS 240
KLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSS
Sbjct: 189 KLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSS 248
Query: 241 GFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQ 300
GFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGV VPYYQ
Sbjct: 249 GFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVIVPYYQ 308
Query: 301 DFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQ 360
DFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQ
Sbjct: 309 DFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQ 368
Query: 361 RFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGET 420
RFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGET
Sbjct: 369 RFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGET 428
Query: 421 ARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPM 480
ARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPM
Sbjct: 429 ARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPM 488
Query: 481 AYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPS 540
AYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPS
Sbjct: 489 AYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPS 548
Query: 541 DGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDG 600
DGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDG
Sbjct: 549 DGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDG 608
Query: 601 GSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPY 660
GSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPY
Sbjct: 609 GSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPY 668
Query: 661 DCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLR 720
DCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLR
Sbjct: 669 DCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLR 728
Query: 721 FENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSFI 769
FENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSFI
Sbjct: 729 FENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSFI 777
BLAST of MC03g_new0248 vs. NCBI nr
Match:
XP_022142562.1 (subtilisin-like protease SBT5.4 [Momordica charantia])
HSP 1 Score: 1035 bits (2676), Expect = 0.0
Identity = 507/774 (65.50%), Postives = 622/774 (80.36%), Query Frame = 0
Query: 1 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 60
+A+ S +FSLL++P+ AAKK YVV+LGSHSHGLE+SE DLQRV +SH++LLGS LGS+
Sbjct: 8 LAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSS 67
Query: 61 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 120
+KAKDAIFYSYKK+INGFAA+LEEE+AA LAKHPEVA VLA++ KLHTTHSW FM LE+
Sbjct: 68 EKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEK 127
Query: 121 KNSSIP-SSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQG---- 180
N +P +S W +A FG+DTIIANLD+GVWPES SF ++GI +P++WKG C
Sbjct: 128 MNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDR 187
Query: 181 FPCNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKN 240
PCNRKLIGAK++N+G++AY++ +N + ++S++ NS RD GHGSHTLSTAGG+FV +
Sbjct: 188 VPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDAS 247
Query: 241 VFGSSGFGMAKGGSPKARLAAYKVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGV 300
VFGS G G AKGGSPKAR+AAYK+CW + GC ADI +G D AIHDGV+V+S S+GG
Sbjct: 248 VFGS-GLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGS 307
Query: 301 NVPYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAV 360
Y+ D AIA+F AVK GI VVCSAGN+GP TA N APW+ TVGASTLDR+ A V
Sbjct: 308 PANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPV 367
Query: 361 QLNNGQRFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILA 420
+L NGQRFQGSSLS LPE+K YPLI+G QA A A DAMLCK LD +K KGKILA
Sbjct: 368 ELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILA 427
Query: 421 CMRGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLD 480
C+RG+ AR+DKG +AA AGA GMILCND+ G T +D H+LPA+H+ Y AV Y+
Sbjct: 428 CLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQ 487
Query: 481 NTQNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHG 540
+TQNPM YL+PP+ K + KPAP MA FSSRGPN++TPEIIKPD+TAPGVN+IAAYT++
Sbjct: 488 STQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVS 547
Query: 541 PSEDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTM 600
P+ +P D R P++TMSGTSMSCPH++G+ GLL++LHP+WSP+AIKSAIMT+AR+RDNTM
Sbjct: 548 PTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTM 607
Query: 601 SPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAF 660
PM+DGG+ PATPFSYGSGH++P+ A+DPGLVYDLT +DYL FLCA+GYD IRAF
Sbjct: 608 KPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAF 667
Query: 661 SDDTPYDCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTV 720
SD PY CPPSASLL+FNYPSIGVQN+ G VTVTR+LKNVG+PGVYRA VR+PEGV V+V
Sbjct: 668 SDG-PYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSV 727
Query: 721 EPRVLRFENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
EPR L+F+ VGEEKSF +T+AG +P VVDGTLIW+DG+HFVRSPIV+SSG F
Sbjct: 728 EPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779
BLAST of MC03g_new0248 vs. NCBI nr
Match:
XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])
HSP 1 Score: 989 bits (2556), Expect = 0.0
Identity = 500/772 (64.77%), Postives = 606/772 (78.50%), Query Frame = 0
Query: 3 VLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKK 62
+LFSF LL++ + AAKK YVVLLGSHSHG+EI+E DL+RV +SH++LLGS LGS +K
Sbjct: 9 ILFSFL---LLISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEK 68
Query: 63 AKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKN 122
AKDAIFYSYKKHINGFAA L+EE+A LA+HPEVA +LA+ LHTTHSW FMHLE KN
Sbjct: 69 AKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLE-KN 128
Query: 123 SSIPSSA-WGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSC----EQGFP 182
IPSS+ W A+ G+D IIANLD+GVWPES SF + GI P+PS+WKG C P
Sbjct: 129 GVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVP 188
Query: 183 CNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVF 242
CNRKLIGAK++N+G++AY+++ N S+V NS RD GHGSHTLSTAGG++V+ +VF
Sbjct: 189 CNRKLIGAKYFNKGFIAYLKSENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVF 248
Query: 243 GSSGFGMAKGGSPKARLAAYKVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNV 302
GS G G AKGGSPKAR+AAYKVCW GC +DI++ D AIHDGVDV+S S+G
Sbjct: 249 GS-GAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPA 308
Query: 303 PYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQL 362
Y +D AIA+F AVK GI VVCSAGN+GP TA N APW+ TVGASTLDR A V+L
Sbjct: 309 EYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVEL 368
Query: 363 NNGQRFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACM 422
NG R+ GSSLSK L K YPLI+G QA A A ++DAMLCK LD +KVKGKILAC+
Sbjct: 369 QNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACL 428
Query: 423 RGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNT 482
RG+ AR+DKG +AA AGAVGMILCND+ G T +D HVLPA+HI Y AV Y+++T
Sbjct: 429 RGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINST 488
Query: 483 QNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPS 542
+NPM YL+PPS K + KPAPTMA FSSRGPNVI+PEIIKPD+TAPGVN+IAA++++ P+
Sbjct: 489 KNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPT 548
Query: 543 EDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSP 602
+ D R VP++TMSGTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMT+AR+RDNTM P
Sbjct: 549 GEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKP 608
Query: 603 MIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSD 662
M+DGGSP PATPF+YGSGH++P A+DPGLVYDL+ DYL FLCA GYD IRAFSD
Sbjct: 609 MLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSD 668
Query: 663 DTPYDCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEP 722
P+ CPP+AS+L+FNYPSIGVQNLNG VTVTRKLKNV +PGVYRA + P+GV V+V+P
Sbjct: 669 G-PFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKP 728
Query: 723 RVLRFENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
+VL+FE V EEKSF +T+ G +P++ VVDG LIW+DGKHFVRSPIV+SS F
Sbjct: 729 KVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 773
BLAST of MC03g_new0248 vs. NCBI nr
Match:
KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])
HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 483/757 (63.80%), Postives = 590/757 (77.94%), Query Frame = 0
Query: 19 AAKK--PYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYSYKKHIN 78
AAKK Y+VLLGSHSHGLE+++ DL+RVA+SH++LLGS+ GS +KA++AIFYSYKK+IN
Sbjct: 2 AAKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNIN 61
Query: 79 GFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPSSAWGKARFG 138
GFAA+++EE+AA LAKHPEVA VL + KLHTTHSW FMHLE+ PSSAW +A+ G
Sbjct: 62 GFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSG 121
Query: 139 EDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQG----FPCNRKLIGAKFYNEGY 198
+D IIANLD+GVWPES SF + GI P+PS+WKG C PCNRKLIGAK++N+G+
Sbjct: 122 KDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGF 181
Query: 199 MAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKGGSPKA 258
+AY+++ N + V NS RD GHGSHTLSTAGG++V+ +VFG G G AKGGSPKA
Sbjct: 182 LAYLKSENL---TALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL-GVGTAKGGSPKA 241
Query: 259 RLAAYKVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDFFAIASFQAV 318
R+AAYKVCW + GC ADI Q D AIHD VDV+S S+GG YY D AI++F AV
Sbjct: 242 RVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAV 301
Query: 319 KNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSLSKGL 378
K GI VVCSAGN+GPG T N APW+ TVGAST+DR A V+L NG R+ GSSLSKGL
Sbjct: 302 KKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGL 361
Query: 379 PEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGTEAAK 438
+K YPLI+G +A A A +A LCK LD +KVKGKIL C+RG+TAR+DKG +AA
Sbjct: 362 KGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAAL 421
Query: 439 AGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPSEKFD 498
AGAVGMILCND+ G T +D HVLPA+HI Y AVF Y+ T+NPM YL+PP+ K +
Sbjct: 422 AGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVN 481
Query: 499 LKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPYMTMS 558
KPAPTMA FSSRGPN+I+PEIIKPD+TAPGVNIIAA++++ P+ +P D R VP++TMS
Sbjct: 482 TKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMS 541
Query: 559 GTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDPATPF 618
GTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMT+AR+RDNT PM+DGGSP P+TPF
Sbjct: 542 GTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPF 601
Query: 619 SYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSASLLDF 678
+YGSGH++P A+DPGLVYDL+ DYL FLCA GY+ I+AFSD P+ CP SAS+L+
Sbjct: 602 AYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDG-PFKCPASASILNL 661
Query: 679 NYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGEEKSFS 738
NYPSIGVQNL G VTVTRKLKNV +PGVY+ VR P GV V V+P+VL+FE VGEEKSF
Sbjct: 662 NYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFE 721
Query: 739 VTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
+T+ G +P++ VVDG LIW+DGKHFVRSPIVVSS F
Sbjct: 722 LTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753
BLAST of MC03g_new0248 vs. NCBI nr
Match:
XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])
HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 479/752 (63.70%), Postives = 587/752 (78.06%), Query Frame = 0
Query: 22 KPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYSYKKHINGFAAV 81
+ Y+VLLGSHSHGLE+++ DL+RVA+SH++LLGS+ GS +KA++AIFYSYKK+INGFAA+
Sbjct: 11 QSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAI 70
Query: 82 LEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPSSAWGKARFGEDTII 141
++EE+AA LAKHPEVA VL + KLHTTHSW FMHLE+ PSSAW +A+ G+D II
Sbjct: 71 MDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII 130
Query: 142 ANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQG----FPCNRKLIGAKFYNEGYMAYMR 201
ANLD+GVWPES SF + GI P+PS+WKG C PCNRKLIGAK++N+G++AY++
Sbjct: 131 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLK 190
Query: 202 NMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKGGSPKARLAAY 261
+ N + V NS RD GHGSHTLSTAGG++V+ +VFG G G AKGGSPKAR+AAY
Sbjct: 191 SENL---TALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL-GVGTAKGGSPKARVAAY 250
Query: 262 KVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDFFAIASFQAVKNGIS 321
KVCW + GC ADI Q D AIHD VDV+S S+GG YY D AI++F AVK GI
Sbjct: 251 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 310
Query: 322 VVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSLSKGLPEEKY 381
VVCSAGN+GPG T N APW+ TVGAST+DR A V+L NG R+ GSSLSKGL +K
Sbjct: 311 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 370
Query: 382 YPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGTEAAKAGAVG 441
YPLI+G +A A A +A LCK LD +KVKGKIL C+RG+TAR+DKG +AA AGAVG
Sbjct: 371 YPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 430
Query: 442 MILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPSEKFDLKPAP 501
MILCND+ G T +D HVLPA+HI Y AVF Y+ T+NPM YL+PP+ K + KPAP
Sbjct: 431 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAP 490
Query: 502 TMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPYMTMSGTSMS 561
TMA FSSRGPN+I+PEIIKPD+TAPGVNIIAA++++ P+ +P D R VP++TMSGTSMS
Sbjct: 491 TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 550
Query: 562 CPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDPATPFSYGSG 621
CPH+SG+VGLL+TLHP WSP+AIKSAIMT+AR+RDNT PM+DGGSP P+TPF+YGSG
Sbjct: 551 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 610
Query: 622 HVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSASLLDFNYPSI 681
H++P A+DPGLVYDL+ DYL FLCA GY+ I+AFSD P+ CP SAS+L+ NYPSI
Sbjct: 611 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDG-PFKCPASASILNLNYPSI 670
Query: 682 GVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGEEKSFSVTLAG 741
GVQNL G VTVTRKLKNV +PGVY+ VR P GV V V+P+VL+FE VGEEKSF +T+ G
Sbjct: 671 GVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITG 730
Query: 742 KLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
+P++ VVDG LIW+DGKHFVRSPIVVSS F
Sbjct: 731 DVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757
BLAST of MC03g_new0248 vs. ExPASy TrEMBL
Match:
A0A6J1CLZ9 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012693 PE=3 SV=1)
HSP 1 Score: 1540 bits (3986), Expect = 0.0
Identity = 768/769 (99.87%), Postives = 768/769 (99.87%), Query Frame = 0
Query: 1 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 60
MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST
Sbjct: 9 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 68
Query: 61 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 120
KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER
Sbjct: 69 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 128
Query: 121 KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNR 180
KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNR
Sbjct: 129 KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNR 188
Query: 181 KLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSS 240
KLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSS
Sbjct: 189 KLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSS 248
Query: 241 GFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQ 300
GFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGV VPYYQ
Sbjct: 249 GFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVIVPYYQ 308
Query: 301 DFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQ 360
DFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQ
Sbjct: 309 DFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQ 368
Query: 361 RFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGET 420
RFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGET
Sbjct: 369 RFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGET 428
Query: 421 ARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPM 480
ARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPM
Sbjct: 429 ARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPM 488
Query: 481 AYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPS 540
AYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPS
Sbjct: 489 AYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPS 548
Query: 541 DGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDG 600
DGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDG
Sbjct: 549 DGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDG 608
Query: 601 GSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPY 660
GSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPY
Sbjct: 609 GSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPY 668
Query: 661 DCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLR 720
DCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLR
Sbjct: 669 DCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLR 728
Query: 721 FENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSFI 769
FENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSFI
Sbjct: 729 FENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSFI 777
BLAST of MC03g_new0248 vs. ExPASy TrEMBL
Match:
A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)
HSP 1 Score: 1035 bits (2676), Expect = 0.0
Identity = 507/774 (65.50%), Postives = 622/774 (80.36%), Query Frame = 0
Query: 1 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 60
+A+ S +FSLL++P+ AAKK YVV+LGSHSHGLE+SE DLQRV +SH++LLGS LGS+
Sbjct: 8 LAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSS 67
Query: 61 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 120
+KAKDAIFYSYKK+INGFAA+LEEE+AA LAKHPEVA VLA++ KLHTTHSW FM LE+
Sbjct: 68 EKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEK 127
Query: 121 KNSSIP-SSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQG---- 180
N +P +S W +A FG+DTIIANLD+GVWPES SF ++GI +P++WKG C
Sbjct: 128 MNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDR 187
Query: 181 FPCNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKN 240
PCNRKLIGAK++N+G++AY++ +N + ++S++ NS RD GHGSHTLSTAGG+FV +
Sbjct: 188 VPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDAS 247
Query: 241 VFGSSGFGMAKGGSPKARLAAYKVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGV 300
VFGS G G AKGGSPKAR+AAYK+CW + GC ADI +G D AIHDGV+V+S S+GG
Sbjct: 248 VFGS-GLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGS 307
Query: 301 NVPYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAV 360
Y+ D AIA+F AVK GI VVCSAGN+GP TA N APW+ TVGASTLDR+ A V
Sbjct: 308 PANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPV 367
Query: 361 QLNNGQRFQGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILA 420
+L NGQRFQGSSLS LPE+K YPLI+G QA A A DAMLCK LD +K KGKILA
Sbjct: 368 ELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILA 427
Query: 421 CMRGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLD 480
C+RG+ AR+DKG +AA AGA GMILCND+ G T +D H+LPA+H+ Y AV Y+
Sbjct: 428 CLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQ 487
Query: 481 NTQNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHG 540
+TQNPM YL+PP+ K + KPAP MA FSSRGPN++TPEIIKPD+TAPGVN+IAAYT++
Sbjct: 488 STQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVS 547
Query: 541 PSEDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTM 600
P+ +P D R P++TMSGTSMSCPH++G+ GLL++LHP+WSP+AIKSAIMT+AR+RDNTM
Sbjct: 548 PTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTM 607
Query: 601 SPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAF 660
PM+DGG+ PATPFSYGSGH++P+ A+DPGLVYDLT +DYL FLCA+GYD IRAF
Sbjct: 608 KPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAF 667
Query: 661 SDDTPYDCPPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTV 720
SD PY CPPSASLL+FNYPSIGVQN+ G VTVTR+LKNVG+PGVYRA VR+PEGV V+V
Sbjct: 668 SDG-PYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSV 727
Query: 721 EPRVLRFENVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
EPR L+F+ VGEEKSF +T+AG +P VVDGTLIW+DG+HFVRSPIV+SSG F
Sbjct: 728 EPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779
BLAST of MC03g_new0248 vs. ExPASy TrEMBL
Match:
A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)
HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 479/752 (63.70%), Postives = 587/752 (78.06%), Query Frame = 0
Query: 22 KPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYSYKKHINGFAAV 81
+ Y+VLLGSHSHGLE+++ DL+RVA+SH++LLGS+ GS +KA++AIFYSYKK+INGFAA+
Sbjct: 11 QSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAI 70
Query: 82 LEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPSSAWGKARFGEDTII 141
++EE+AA LAKHPEVA VL + KLHTTHSW FMHLE+ PSSAW +A+ G+D II
Sbjct: 71 MDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVII 130
Query: 142 ANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQG----FPCNRKLIGAKFYNEGYMAYMR 201
ANLD+GVWPES SF + GI P+PS+WKG C PCNRKLIGAK++N+G++AY++
Sbjct: 131 ANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLK 190
Query: 202 NMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKGGSPKARLAAY 261
+ N + V NS RD GHGSHTLSTAGG++V+ +VFG G G AKGGSPKAR+AAY
Sbjct: 191 SENL---TALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL-GVGTAKGGSPKARVAAY 250
Query: 262 KVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDFFAIASFQAVKNGIS 321
KVCW + GC ADI Q D AIHD VDV+S S+GG YY D AI++F AVK GI
Sbjct: 251 KVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIP 310
Query: 322 VVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSLSKGLPEEKY 381
VVCSAGN+GPG T N APW+ TVGAST+DR A V+L NG R+ GSSLSKGL +K
Sbjct: 311 VVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKL 370
Query: 382 YPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGTEAAKAGAVG 441
YPLI+G +A A A +A LCK LD +KVKGKIL C+RG+TAR+DKG +AA AGAVG
Sbjct: 371 YPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVG 430
Query: 442 MILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPSEKFDLKPAP 501
MILCND+ G T +D HVLPA+HI Y AVF Y+ T+NPM YL+PP+ K + KPAP
Sbjct: 431 MILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAP 490
Query: 502 TMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPYMTMSGTSMS 561
TMA FSSRGPN+I+PEIIKPD+TAPGVNIIAA++++ P+ +P D R VP++TMSGTSMS
Sbjct: 491 TMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMS 550
Query: 562 CPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDPATPFSYGSG 621
CPH+SG+VGLL+TLHP WSP+AIKSAIMT+AR+RDNT PM+DGGSP P+TPF+YGSG
Sbjct: 551 CPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSG 610
Query: 622 HVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSASLLDFNYPSI 681
H++P A+DPGLVYDL+ DYL FLCA GY+ I+AFSD P+ CP SAS+L+ NYPSI
Sbjct: 611 HIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDG-PFKCPASASILNLNYPSI 670
Query: 682 GVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGEEKSFSVTLAG 741
GVQNL G VTVTRKLKNV +PGVY+ VR P GV V V+P+VL+FE VGEEKSF +T+ G
Sbjct: 671 GVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITG 730
Query: 742 KLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
+P++ VVDG LIW+DGKHFVRSPIVVSS F
Sbjct: 731 DVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757
BLAST of MC03g_new0248 vs. ExPASy TrEMBL
Match:
A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)
HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 480/750 (64.00%), Postives = 577/750 (76.93%), Query Frame = 0
Query: 24 YVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYSYKKHINGFAAVLE 83
YVVLLGSHSHGLE++E D + V +SH++LLGS L S +KAKD+IFYSYKKHINGFAA L+
Sbjct: 756 YVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATLD 815
Query: 84 EEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPSSAWGKARFGEDTIIAN 143
+EDA LA HPEVA VL ++ L+TTHSW FMHLE+ PSS W A+FG+D IIAN
Sbjct: 816 DEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIAN 875
Query: 144 LDSGVWPESMSFRDSGIRKPIPSRWKGSCEQ----GFPCNRKLIGAKFYNEGYMAYMRNM 203
LD+GVWPES SF + GI P PS+WKG C G PCN+KLIGAK++N+GY+ Y+++
Sbjct: 876 LDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKSE 935
Query: 204 NPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKGGSPKARLAAYKV 263
N ++S++ NS RD GHGSHTLSTA GN+V +VFGS G G AKGGSPKAR+AAYKV
Sbjct: 936 NSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGS-GIGTAKGGSPKARVAAYKV 995
Query: 264 CWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDFFAIASFQAVKNGISVV 323
CW GC ADI + D AIHDGVDV+S S+GG + Y +D AIASF AVK GI VV
Sbjct: 996 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 1055
Query: 324 CSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSLSKGLPEEKYYP 383
C+ GN+GP P TA N APW+ TVGASTLDR A V L NG RF GSS SKGL K YP
Sbjct: 1056 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 1115
Query: 384 LISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGTEAAKAGAVGMI 443
LI+G QA AG A +DAMLCK LD +KVKGKIL C+RGETAR+DKG +AA AGAVGMI
Sbjct: 1116 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 1175
Query: 444 LCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPSEKFDLKPAPTM 503
LCND+ G D H+LPA+HI YQ + Y+++ +NPM YL+PP K + KPAPTM
Sbjct: 1176 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 1235
Query: 504 ADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPYMTMSGTSMSCP 563
A FSSRGPN I+PEIIKPD+TAPGVNIIAA++++ P+ D SD R P++TMSGTSMSCP
Sbjct: 1236 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 1295
Query: 564 HISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDPATPFSYGSGHV 623
H++G+VGLL+ LHPDWSP+AIKSAIMT+++VRDNT++PMIDGGS PATPF+YGSGH+
Sbjct: 1296 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 1355
Query: 624 QPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSASLLDFNYPSIGV 683
P A+DPGLVYDL+ DYL FLCA GYD IRAFSD+ P+ CPP++S+L+ NYPSIGV
Sbjct: 1356 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDE-PFKCPPASSVLNLNYPSIGV 1415
Query: 684 QNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGEEKSFSVTLAGKL 743
QNL G V+VTRKLKNVGSPGVYRA + P GV V+V+PR L+FE VGEEKSF +TLAG +
Sbjct: 1416 QNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVV 1475
Query: 744 PQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
P++ V G LIWSDG+H VRSPIVVSSG F
Sbjct: 1476 PKDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503
BLAST of MC03g_new0248 vs. ExPASy TrEMBL
Match:
A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)
HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 484/766 (63.19%), Postives = 593/766 (77.42%), Query Frame = 0
Query: 10 FSLLL--TPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAI 69
FSLLL +P+ AAKK Y+VLLGSHSHGLE+ + DL+RVA+SH++LLGS++GS +KA+DAI
Sbjct: 16 FSLLLVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSIIGSDEKARDAI 75
Query: 70 FYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPS 129
FYSYK++INGFAA+++EE+A LAKHPEVA VL ++ KLHTTHSW FMHLE+ PS
Sbjct: 76 FYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMHLEKNGVIPPS 135
Query: 130 SAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQ----GFPCNRKLI 189
SAW +A+ G+D II NLD+GVW ES SF + GI +PS+WKG C G CNRKLI
Sbjct: 136 SAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTPDGVSCNRKLI 195
Query: 190 GAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFG 249
GAK++N+G++AY+ + N ++V NS RD GHGSHTLSTAGG++V+ +VFG G G
Sbjct: 196 GAKYFNKGFLAYLNSQNL---TASVINSTRDYDGHGSHTLSTAGGSYVSGASVFGL-GVG 255
Query: 250 MAKGGSPKARLAAYKVCWG-SNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDF 309
AKGGSPKAR+A+YKVCW + GC ADI + D AIHD VDV+S S+GG YY D
Sbjct: 256 TAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDG 315
Query: 310 FAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRF 369
AIA+F AVK GI VVCSAGN+GP T N APW+ TVGASTLDR A V+L NG +
Sbjct: 316 IAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAPVELQNGHSY 375
Query: 370 QGSSLSKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETAR 429
GSSLSKGL +K YPLI+G +A A A AMLCK LD +KVKGKIL C+RG+TAR
Sbjct: 376 MGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTAR 435
Query: 430 IDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAY 489
+DKG +AA AGAVGMILCND+ G T +D HVLPA+HI Y AVF Y+ +T+NPM
Sbjct: 436 VDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGS 495
Query: 490 LVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDG 549
L+PPS K + KPAP+MA FSSRGPN+I+PEIIKPD+TAPGVNIIAA++++ P+ +P D
Sbjct: 496 LIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDN 555
Query: 550 RRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGS 609
R VP++TMSGTSMSCPH+SG+VGLL+TLHP WSP+AIKSAIMT+AR+RDNT PM+DGGS
Sbjct: 556 RTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTKKPMLDGGS 615
Query: 610 PRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDC 669
P PATPF+YGSGH++P A+DPGLVYDL+ DYL FLCA GY+ I+AFSD P+ C
Sbjct: 616 PDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDG-PFKC 675
Query: 670 PPSASLLDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFE 729
P SAS+L+FNYPSIGVQNL G VT+TRKLKNV +PGVY+A V P GV V V+P+VL+FE
Sbjct: 676 PASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFE 735
Query: 730 NVGEEKSFSVTLAGKLPQNAVVDGTLIWSDGKHFVRSPIVVSSGSF 768
VGEEK F + + G +P+N VVDG LIW+DGKHFVRSPIVVSSG F
Sbjct: 736 RVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776
BLAST of MC03g_new0248 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 855.9 bits (2210), Expect = 2.5e-248
Identity = 441/760 (58.03%), Postives = 535/760 (70.39%), Query Frame = 0
Query: 11 SLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGSTKKAKDAIFYS 70
+L +P+FA KK Y+V LGSH+H +IS L VA+SH L S +GS + AK+AIFYS
Sbjct: 29 TLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYS 88
Query: 71 YKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPSSAW 130
YK+HINGFAA+L+E +AA +AKHP+V V ++ KLHTTHSW FM L + SS W
Sbjct: 89 YKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLW 148
Query: 131 GKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFPCNRKLIGAKFYNE 190
KA +GEDTIIANLD+GVWPES SF D G +P+RWKG C + PCNRKLIGA+++N+
Sbjct: 149 NKAGYGEDTIIANLDTGVWPESKSFSDEGY-GAVPARWKGRCHKDVPCNRKLIGARYFNK 208
Query: 191 GYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGFGMAKGGSP 250
GY+AY + N + T RD GHGSHTLSTA GNFV NVFG G G A GGSP
Sbjct: 209 GYLAY-TGLPSNASYET----CRDHDGHGSHTLSTAAGNFVPGANVFG-IGNGTASGGSP 268
Query: 251 KARLAAYKVCWGSNVG--CPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQDFFAIASF 310
KAR+AAYKVCW G C ADIL ++ AI DGVDV+S S+GG Y D AI SF
Sbjct: 269 KARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSF 328
Query: 311 QAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQRFQGSSLS 370
AVKNG++VVCSAGN+GP GT N+APW+ TVGAS++DR A V+L NGQ F+G+SLS
Sbjct: 329 HAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS 388
Query: 371 KGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGETARIDKGTE 430
K LPEEK Y LIS A + DA+LCK G+LDP KVKGKIL C+RG+ AR+DKG +
Sbjct: 389 KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQ 448
Query: 431 AAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNPMAYLVPPSE 490
AA AGA GM+LCND+ G SD HVLPA+ I Y+ +F YL +T++P Y+ P+
Sbjct: 449 AAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTA 508
Query: 491 KFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDPSDGRRVPYM 550
+ KPAP MA FSSRGPN ITP I+KPDITAPGVNIIAA+T++ GP++ SD RR P+
Sbjct: 509 TLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFN 568
Query: 551 TMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMIDGGSPRGDPA 610
T SGTSMSCPHISGVVGLLKTLHP WSPAAI+SAIMTT+R R+N PM+D + A
Sbjct: 569 TESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKK---A 628
Query: 611 TPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTPYDCPPSASL 670
PFSYGSGHVQP A PGLVYDLTT DYL+FLCA+GY+ ++++ F++D Y C A+L
Sbjct: 629 NPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANL 688
Query: 671 LDFNYPSIGVQNLNGQVTVTRKLKNVGSPGVYRASVRRPEGVAVTVEPRVLRFENVGEEK 730
LDFNYPSI V NL G +TVTRKLKNVG P Y A R P GV V+VEP+ L F GE K
Sbjct: 689 LDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVK 748
Query: 731 SFSVTLAG-KLPQNAVVDGTLIWSDGKHFVRSPIVVSSGS 768
F +TL + + V G L W+D H+VRSPIVV S
Sbjct: 749 IFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQLSS 778
BLAST of MC03g_new0248 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 748.8 bits (1932), Expect = 4.3e-216
Identity = 407/776 (52.45%), Postives = 518/776 (66.75%), Query Frame = 0
Query: 5 FSFFVFSLLLTPS----FAAK--KPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLG 64
FSF + LL+ S A+K YVV G+HSH EI+E + RV +HY LGS G
Sbjct: 7 FSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTG 66
Query: 65 STKKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHL 124
S ++A DAIFYSY KHINGFAA L+ + A ++KHPEV V ++ +KLHTT SW F+ L
Sbjct: 67 SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 126
Query: 125 ERKNSSIPSSA-WGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQ--- 184
E NS +PSS+ W KARFGEDTIIANLD+GVWPES SFRD G+ PIPSRWKG C+
Sbjct: 127 EH-NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKD 186
Query: 185 -GFPCNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAA 244
F CNRKLIGA+++N+GY A + ++N +F +S RD GHGSHTLSTA G+FV
Sbjct: 187 ATFHCNRKLIGARYFNKGYAAAVGHLNSSF------DSPRDLDGHGSHTLSTAAGDFVPG 246
Query: 245 KNVFGSSGFGMAKGGSPKARLAAYKVCWGSNVG--CPGADILQGLDDAIHDGVDVVSYSI 304
++FG G G AKGGSP+AR+AAYKVCW G C AD+L D AIHDG DV+S S+
Sbjct: 247 VSIFG-QGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSL 306
Query: 305 GGVNVPYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIV 364
GG ++ D AI SF A K I VVCSAGN+GP T N+APW TVGAST+DR
Sbjct: 307 GGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFA 366
Query: 365 AAVQLNNGQRFQGSSLSK-GLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKG 424
+ + L NG+ ++G SLS LP K+YP+++ A A A DA LCK G+LDP K KG
Sbjct: 367 SNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKG 426
Query: 425 KILACMRGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVF 484
KIL C+RG+ R++KG A G +GM+L N G +D HVLPAT + + S AV
Sbjct: 427 KILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVS 486
Query: 485 DYLDNTQNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYT 544
Y+ T+ P+A++ P LKPAP MA FSS+GP+++ P+I+KPDITAPGV++IAAYT
Sbjct: 487 RYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYT 546
Query: 545 QSHGPSEDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVR 604
+ P+ + D RR+ + +SGTSMSCPHISG+ GLLKT +P WSPAAI+SAIMTTA +
Sbjct: 547 GAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIM 606
Query: 605 DNTMSPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSM 664
D+ P+ + + + ATPFS+G+GHVQP A++PGLVYDL +DYLNFLC+LGY+ S
Sbjct: 607 DDIPGPIQNATNMK---ATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQ 666
Query: 665 IRAFSDDTPYDCPPSASLLDFNYPSIGVQNL-NGQVTVTRKLKNVGSPGVYRASVRRPEG 724
I FS + P SL++ NYPSI V NL + +VTV+R +KNVG P +Y V P+G
Sbjct: 667 ISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQG 726
Query: 725 VAVTVEPRVLRFENVGEEKSFSVTLAGKLPQNA--VVDGTLIWSDGKHFVRSPIVV 764
V V V+P L F VGE+K+F V L A V G L+WSD KH VRSPIVV
Sbjct: 727 VYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVV 770
BLAST of MC03g_new0248 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 558.5 bits (1438), Expect = 8.2e-159
Identity = 339/791 (42.86%), Postives = 451/791 (57.02%), Query Frame = 0
Query: 1 MAVLFSFFVFSLLLTPSFAAKKPYVVLLGSHSHGLEISEVDLQRVANSHYRLLGSLLGST 60
MA F FF L +PS +A S ++ + + + +H+ S L S
Sbjct: 1 MAFFFYFFFLLTLSSPSSSASSS-----NSLTYIVHVDHEAKPSIFPTHFHWYTSSLASL 60
Query: 61 KKAKDAIFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLER 120
+ +I ++Y +GF+A L +DA+ L HP V V+ +++ LHTT S F+ L
Sbjct: 61 TSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGL-- 120
Query: 121 KNSSIPSSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSC--EQGFP- 180
S+ + ++ FG D +I +D+GVWPE SF D G+ P+P +WKG C Q FP
Sbjct: 121 -RSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGL-GPVPIKWKGQCIASQDFPE 180
Query: 181 --CNRKLIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKN 240
CNRKL+GA+F+ GY A N N +T S RD GHG+HT S + G +V +
Sbjct: 181 SACNRKLVGARFFCGGYEA----TNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPAS 240
Query: 241 VFGSSGFGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVN 300
G + G+A G +PKARLAAYKVCW N GC +DIL D A+ DGVDV+S S+GGV
Sbjct: 241 TLGYA-HGVAAGMAPKARLAAYKVCW--NSGCYDSDILAAFDTAVADGVDVISLSVGGVV 300
Query: 301 VPYYQDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQ 360
VPYY D AI +F A+ GI V SAGN GPG T N+APW+ TVGA T+DR A V+
Sbjct: 301 VPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVK 360
Query: 361 LNNGQRFQGSSL--SKGLPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKIL 420
L NG+ G S+ GL + YPL+ GG G Y + LC G+LDP VKGKI+
Sbjct: 361 LGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY--SSSLCLEGSLDPNLVKGKIV 420
Query: 421 ACMRGETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYL 480
C RG +R KG K G +GMI+ N DGEG +D HVLPAT +G + Y+
Sbjct: 421 LCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYI 480
Query: 481 D------NTQNPMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIA 540
++++P A +V + ++PAP +A FS+RGPN TPEI+KPD+ APG+NI+A
Sbjct: 481 SESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILA 540
Query: 541 AYTQSHGPSEDPSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTA 600
A+ GPS SD RR + +SGTSM+CPH+SG+ LLK HPDWSPAAI+SA++TTA
Sbjct: 541 AWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTA 600
Query: 601 RVRDNTMSPMIDGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYD 660
DN+ PM+D + G+ ++ YGSGHV P AMDPGLVYD+T+ DY+NFLC Y
Sbjct: 601 YTVDNSGEPMMDEST--GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYT 660
Query: 661 WSMIRAFSDDTPYDCP---PSASLLDFNYPSIGV--QNLNGQVTVT---RKLKNVG-SPG 720
+ I + DC + + + NYPS V Q T R + NVG S
Sbjct: 661 RTNIVTIT-RRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDS 720
Query: 721 VYRASVRRPEGVAVTVEPRVLRFENVGEEKSFSV---TLAGKLPQNA--VVDGTLIWSDG 765
VY +R P G VTVEP L F VG++ SF V T KL A V G ++WSDG
Sbjct: 721 VYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDG 770
BLAST of MC03g_new0248 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 557.8 bits (1436), Expect = 1.4e-158
Identity = 326/712 (45.79%), Postives = 430/712 (60.39%), Query Frame = 0
Query: 67 IFYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIP 126
+ Y+Y+ I+GF+ L +E+A +L P V VL + +LHTT + F+ L+ + +
Sbjct: 65 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL- 124
Query: 127 SSAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGFP-----CNRK 186
+ +A D ++ LD+GVWPES S+ D G PIPS WKG CE G CNRK
Sbjct: 125 ---FPEAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTASLCNRK 184
Query: 187 LIGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSG 246
LIGA+F+ GY + M P + S + S RDD GHG+HT STA G+ V ++ G +
Sbjct: 185 LIGARFFARGYES---TMGP-IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYAS 244
Query: 247 FGMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIGGVNVPYYQD 306
G A+G +P+AR+A YKVCW GC +DIL +D AI D V+V+S S+GG YY+D
Sbjct: 245 -GTARGMAPRARVAVYKVCWLG--GCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 304
Query: 307 FFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNGQR 366
AI +F A++ GI V CSAGNAGP + N+APW+ TVGA TLDR A L NG+
Sbjct: 305 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 364
Query: 367 FQGSSLSKG--LPEEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRGE 426
F G SL KG LP +K P I G A+ + LC G L P KVKGKI+ C RG
Sbjct: 365 FTGVSLFKGEALP-DKLLPFIYAGNASNA----TNGNLCMTGTLIPEKVKGKIVMCDRGI 424
Query: 427 TARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQNP 486
AR+ KG AG VGMIL N +GE +D H+LPAT +G +A + Y+ NP
Sbjct: 425 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 484
Query: 487 MAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSEDP 546
A + +KP+P +A FSSRGPN ITP I+KPD+ APGVNI+AA+T + GP+
Sbjct: 485 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 544
Query: 547 SDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMID 606
SD RRV + +SGTSMSCPH+SG+ LLK++HP+WSPAAI+SA+MTTA P++D
Sbjct: 545 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 604
Query: 607 GGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDTP 666
+ G P+TPF +G+GHV P A +PGL+YDLTTEDYL FLCAL Y IR+ S
Sbjct: 605 IAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN- 664
Query: 667 YDCPPSA--SLLDFNYPSIGVQNLN--GQVTVTRKLKNVGSPGVYRASV-RRPEGVAVTV 726
Y C PS S+ D NYPS V N++ G TR + +VG G Y V GV ++V
Sbjct: 665 YTCDPSKSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISV 724
Query: 727 EPRVLRFENVGEEKSFSVTLA--GKLPQNAVVDGTLIWSDGKHFVRSPIVVS 765
EP VL F+ E+KS++VT P + G++ WSDGKH V SP+ +S
Sbjct: 725 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of MC03g_new0248 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-152
Identity = 328/780 (42.05%), Postives = 449/780 (57.56%), Query Frame = 0
Query: 12 LLLTPSFAAKKPYVVLLGSHSHGLEISEVD---LQRVANSHYRLLGSLLGSTKKAKDA-I 71
LLL SF P++ S + I +D + + +HY ST+ A+++ I
Sbjct: 8 LLLFLSF----PFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWY-----STEFAEESRI 67
Query: 72 FYSYKKHINGFAAVLEEEDAAALAKHPEVAVVLADEMMKLHTTHSWGFMHLERKNSSIPS 131
+ Y +GF+AV+ ++A L HP V V D +LHTT S F+ L+ +
Sbjct: 68 VHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQ-----K 127
Query: 132 SAWGKARFGEDTIIANLDSGVWPESMSFRDSGIRKPIPSRWKGSCEQGF-----PCNRKL 191
W ++ +G D II D+G+WPE SF D + PIP RW+G CE G CNRK+
Sbjct: 128 GLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGARFSPRNCNRKI 187
Query: 192 IGAKFYNEGYMAYMRNMNPNFNVSTVANSARDDAGHGSHTLSTAGGNFVAAKNVFGSSGF 251
IGA+F+ +G A + N + S RD GHG+HT STA G ++ G +
Sbjct: 188 IGARFFAKGQQAAVIG---GINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYAS- 247
Query: 252 GMAKGGSPKARLAAYKVCWGSNVGCPGADILQGLDDAIHDGVDVVSYSIG---GVNVPYY 311
G+AKG +PKAR+AAYKVCW + GC +DIL D A+ DGVDV+S SIG G+ PYY
Sbjct: 248 GVAKGVAPKARIAAYKVCW-KDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYY 307
Query: 312 QDFFAIASFQAVKNGISVVCSAGNAGPGPGTAVNLAPWLFTVGASTLDRRIVAAVQLNNG 371
D AI S+ A GI V SAGN GP + NLAPW+ TVGAST+DR A L +G
Sbjct: 308 LDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDG 367
Query: 372 QRFQGSSLSKGLP-EEKYYPLISGGQAAAGGAYINDAMLCKAGALDPAKVKGKILACMRG 431
R +G SL G+P + +P++ G++ ++ A LC LDP +V+GKI+ C RG
Sbjct: 368 HRLRGVSLYAGVPLNGRMFPVVYPGKSG-----MSSASLCMENTLDPKQVRGKIVICDRG 427
Query: 432 ETARIDKGTEAAKAGAVGMILCNDQRDGEGTYSDMHVLPATHIGYQASLAVFDYLDNTQN 491
+ R+ KG KAG VGMIL N +GEG D H++PA +G + Y + N
Sbjct: 428 SSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPN 487
Query: 492 PMAYLVPPSEKFDLKPAPTMADFSSRGPNVITPEIIKPDITAPGVNIIAAYTQSHGPSED 551
P+A + +KPAP +A FS RGPN ++PEI+KPD+ APGVNI+AA+T + GP+
Sbjct: 488 PIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGL 547
Query: 552 PSDGRRVPYMTMSGTSMSCPHISGVVGLLKTLHPDWSPAAIKSAIMTTARVRDNTMSPMI 611
PSD R+ + +SGTSM+CPH+SG LLK+ HPDWSPA I+SA+MTT + DN+ +I
Sbjct: 548 PSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLI 607
Query: 612 DGGSPRGDPATPFSYGSGHVQPVAAMDPGLVYDLTTEDYLNFLCALGYDWSMIRAFSDDT 671
D + G ATP+ YGSGH+ AM+PGLVYD+T +DY+ FLC++GY I+ + T
Sbjct: 608 DEST--GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVIT-RT 667
Query: 672 PYDCPPS--ASLLDFNYPSIGV---QNLNGQV--TVTRKLKNVG-SPGVYRASVRRPEGV 731
P CP + S + NYPSI N G V TV R NVG + VYRA + P GV
Sbjct: 668 PVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGV 727
Query: 732 AVTVEPRVLRFENVGEEKSFSVTLAGK-----LPQNAVVDGTLIWSD-GKHFVRSPIVVS 765
VTV+P L F + + +S++VT+ L + V G++ W D GKH VRSPIVV+
Sbjct: 728 TVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| F4JXC5 | 3.5e-247 | 58.03 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| Q9ZSP5 | 6.0e-215 | 52.45 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| I1N462 | 9.4e-184 | 46.96 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| Q9LUM3 | 1.2e-157 | 42.86 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
| O65351 | 2.0e-157 | 45.79 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CLZ9 | 0.0 | 99.87 | subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012693 P... | [more] |
| A0A6J1CN43 | 0.0 | 65.50 | subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 P... | [more] |
| A0A0A0LVY8 | 0.0 | 63.70 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1 | [more] |
| A0A5D3CZ66 | 0.0 | 64.00 | Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A1S3CLB7 | 0.0 | 63.19 | subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=... | [more] |
