MC03g_new0203 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0203
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	tRNA ligase 1
Location	MC03: 9039086 .. 9043878 (-)
RNA-Seq Expression	MC03g_new0203
Synteny	MC03g_new0203

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGCGTCGCACAGGATCTTCTGCGCCATCACTCTCCCTCACCCTCCTCGTTTCTCGCCCTCTTCACTCTTCAATTCCAGGGCCTTCCTCTCCACTTCCCACTTCATTTTTCCCCGCTCTCTCGCCCTCCCGCCTCTAATTTCTTCCCCTTTTCACCTTTCTCCTCATTCTCGATCGATCATGCCTTACAATCAGGTACCCCCCACGTTTTTGTTTGATGGGTTTTGTTCGTCATCGTTGTTTCCCGCTTTCCGGGTTGTGAATTTATGCACATGATCGCCCATTTCTGTATTGGGGTGTCTCTGTTTGTTTTCTTCAATGCTTTTCATGTTGGTGATTGTCTCGTTTTGTTGTTGACATAAATGCGGTAATCTGTTTCTTTGATATGGGGACAGGGCAGGTTTGAATTTGTTATCTGTTTGTTTGTTCCGTTTTCTTCCACTCAGTCTTTGGGAATTATGTTTTATGCCCTTTTCGGCGTTTGATGGTTTACGTGGATAGAGATGTTGATATTATTACAATGCTGTCATGTAAGAGGATTCAACTGTAGTAATTGGTGGTTTTTTTATACGTATTGACGCATATGGAACAGTTCATTTCTGTTACTTTTGTCTCAAGTCATCGGCAATGCATAATTAGAATTTTATCTTTGGTTCGGGATTATCTGCTTGTGAATGTTCAGCTTATTGAGTTTGATCTTAATTTGCAGCGAAGTGATGGTCGCAGAGAACAAAAGTGGAAAGAGAAGGCGAAGCTTGACAGAACTTCTACAGAGTCAGAGGCTGCTGCTGAAGTTGTCACTAATGCACTTGGTAAATTGAGAGTCAGTGAAAGTGGTCAACCTCATGTTCCTATTTCTAGTAGGGAGTTTGGAAATGCGCAGCTAACAAACCAGGTCCCCTCTGGGCTTGGAAATAGAGGGATTTGGAAACCGAAAGCATATGGAACAACCAGTGGGGGGGCAGCAGTGGTTGAAGCTGAAAAGGCACCTGCCGTTGGAACATCTATTGAAAACAAGGGGAACACTGCTGGACTAGCTGCACAGAATGGCACTGTTGGCTTGAGTCAACTATTCAAGGGCAATCAGATAGAAAATTTTACTGTGGACAACTCCACTTACACACAGGCACAAATAAGAGCTACGTTCTATCCTAAATTTGAGAACGAGAAGTCAGATCAGGAGGTATTTCTTTATTCTATGTGCTTCCTTAAATTTACACCCTGGATATGCTTTATTGGACCTAATTTTTTTTTATCTTTAACTCAGTTGTTAGATTAACTATTCAACCTCCAAGATGTTTTTTCCCATTAACCATGTCATTGGCTTGTTGGGTCAGTGTTTCCAGGTGCATGCTCGGGGCCATTTACTTGTTTTCCAATCTCTTGACCATCATCTTTAGGTCATTCGTCAACTCTGGTTTTGCCCGTTTTTGTTTTGTTTTTGGGTTAAAAACATTTTGTTTTCCAATCTCTTGACCATCATCTTTAGGTCATTCGTCAACTCTGGTTTTGCCCGTTTTTGTTTTGTTTTTGGGTTAAAAACATTTAATAAAATAGCAAGAAAGGTAACCATCATGTGATGGCTCAAGTGGTCAATGAGGGCCAATGTAAATAGCAAAGGACTTAGAGGGAATGAGTTCAAACCATGGTGGCCACCTACCTAGAATTTAATATCCTATGAGTTTCCTTGTCAACCAAATGTAGTAGAGTTTGACGGTTGGCCCGTGAATAGTCAAGGTGTGCATAAGCTAGCCCGGACACAGGGATATAAAAAAAAATGTAAGAAATGTCTAATAAAGTGAATAGAAAGGACCAGGTATAAGAGGATAATGAATCCTCTTAAAGTCACAGATATCTTCGTGTTACAAAATAAGTACCAATTCAGAGTGCAAGGAGGCAAGAGGAATACCAAAATTTTAAAGACCAACGTTTATATTCTGAAGCAATGTAAAAAATTGAACTCAAAGAAAATCACAAAAAGGAAATTAATTATTAGTTCATGTATTTTTAATGAGATAACAAATGAGCGAATACACGAGGGCTATACAAAAAGAAAGGAAAGCTCAAGCTAGAAGGAGCCAAAACAACAATAAAAACACAAAAGAAAGAATTATTATAGAGGGCTCCAATTTAAAATAACGAGAGATGAAGGGTAATTGCTAAAGACCTTGGTAATGAGAGATGAAGGGTAATTGCTAAAGACCTTGTAATGGAAGCCCAAAGGGACGCATTGAACCTAACCAAGCCTCCAACCTCCTCCTAGGAGCTTTTCTTATCACGAAAGAATCCTTTTTCCTTCAAGAAAAATATTCCACAAAGTTGCGAGAAACCAGCCAGCCAAATAAATGCCCCTCAGTTATTAAAGGTGATGGAACAATACCTCCTCCATCATAAATAACTGGTTCATGTTAGTCACATATAAAAGAATTTGATAGATTCGTATATTTTACAATTTACGATCATGATTACACCATATTTAATAAAAATTTCCATTATGGTAAGAAAATGAAGGTGATAGTTCTTTTGTGAAACAGAAAATTGTTTTAGAATCCAAATCATTGGGTAATTTGAGTGTTGGCGATTTAAAAATCTAAGCAACAACATATTATGCTTCTATTGTTGAATCAGCAGTACAACCTCGCTATAACATTTCCCCACATTATGTCTCCACCACCCAAGGTGAAAATTGATCTTATGTGGATTTTCTGGAATCCTTTGGTATAAATTGTTTATGGATAAATGGATGGGGAAGTTTTCTCATGAACCTTTTACGTTTATGGCTTCCACTGGACATTTGTCTTTTTGCAATGTGCTTCATATTTAATAAAATTTTCCATTATGGGCAAAAAAGGTTAAATGGTGATTCTGTGGGTAATTTAATTTTAAACTTACGATTTTTGTTAAGGCAACATTTTCTATATTTTATTAGATAGATTATTAATTTCATTGCCAATGATGAGAGAATTTCTTTCCTGGTAATATGTTTAATGTGCATATGATTTTATGTCAGATTAGAACAAGGATGATAGAGATGGTATCGAAAGGCTTGGCAACATTGGAGGTAAAGAGATTTACCCGCAATTTTTGTCAAGAATGCCATAATGTTATGATACTTTATATTCCATTTATGATGGAGTTACTACGTATTTTTGAGTTTTGACTCTCATTCACTCCCAATTCTGGATGTTAAACCTTTAATGAGAGTAGATTCGAAATGAAAGTATATTGATTGATTTGGACATTGATTCATTTTTGGGTGTTGTGTTGGCGACTACGCATTTTTATGATTTTTTATGCTTTAAAACTGCAAAAGTGGGCCCATAGTTGGAGGTAAAGAGATTTACCTGCAATTTTTGTCAAGAATGCCATAATGTTATGGTACTTTATATTCCATTTATGATGGAGTTACTACGTATTTTTGACTGTCATTCACTCCCAATTCTGGATGTTAAACCTTGGATGAGAGTACTTTCAAAGTGAAAGTATATTGAGTGATTTGGACATTGATTCATTTTTGGGTGTTGTGTCAGTGACTACGCATTTTTATGAATTTTTATGCTTTAAAATTGCAAAATTNGGGGGGGGGGGGGGGGGGGGGGGGGGGGCTTGGTTTTAAAAATTTCGTTGCTTATAGAACAAAATAGTAGTAAAAATAGTTTTTGTAGCGTTTATCACTTTTTATAATAATTTGTAGCGAGTACTATTTCCAATTTCCCCCCTTGTCACTACTTTGCTTATAGTGTTCGGATACTGTCTCTAATGGTAAATAGTATATTGATTGTGTTCTGAATTCTTTAAGTTGTTATTAGGTTATTCAACTTGGAAATTTCTGCAGGTTTCACTTAAACACTCAGGGTCTTTGTTTATGTACGCTGGCCATGAAGGTGGAGCATACGCCAAAAACAGCTTCGGGAATATGTATGCCAATAATATGTTTTTTCTTTTAATTATTTTTTGGATGGTGTGCGGGACACTGATATGCCAAAATCCATTTTGATAGAGCCAGTAAGGAATTTAATTTGAAGTGGATGTAATCATACTTTTTGCTATTCTTTCTTGAATAAGGAAATTTACATAATTATTGTGTGAACATGTATGTCTCCACAAACTTGTTACAATTTCCATTTGCCATGATTTTTTGTTTTGTTTTGTTTTTTGTTACAATTTCCACTTGTTACAAAGTTAAAACTCCCGACCTGTTTCCTAAGTTAAAAGTTTCACTTTCAGATACACTGCTGTTGGTGTTTTTGTTCTGGGAAGGATGTTTCGAGAGGCCTGGGGATCTAAAGCAGCAAAAAAGCAAGCAGAATTCAATAATTTTCTTGAGGTATTGTTAACATTAGAGAAACAATGATTTTGTTATCTTACATTTTTCCTTATGATAAGAGAGTGTTACACGATTACTTAACAAAGGACCTCCCTTGATATTTAACAAATCTGCTTCATGGGTCCATATACAATCTCATGGTATTTCAGAGTATCAAATCTTGTTAGATCAATTTCTGTGAAATCCCTCCTAATGATAAGTTCACTTGGTCTAGCTTTAGGGAGGATGTAGTTTGGTCTTTAACGGTAGATTCCTTTTTTTCTGGAGTAGGAGGGTAAATTCAAGCATCTGAAAGTGATAAGAATGCTAAAAAATACGTTGGGTAATTTTTTGATTGTGTTAGAAACTGGAGATCATGGGAACCATGTCGTTTAGGTTTGTTTTCTTCACTCTTTTGAAGCTTGGTGGTAGGACACCAAATGGAAGGATGGCCCAGCTTTCTCTTTCCCTCAGAAAGTCATAA

mRNA sequence

ATGTCGGCGTCGCACAGGATCTTCTGCGCCATCACTCTCCCTCACCCTCCTCGTTTCTCGCCCTCTTCACTCTTCAATTCCAGGGCCTTCCTCTCCACTTCCCACTTCATTTTTCCCCGCTCTCTCGCCCTCCCGCCTCTAATTTCTTCCCCTTTTCACCTTTCTCCTCATTCTCGATCGATCATGCCTTACAATCAGCTTATTGAGTTTGATCTTAATTTGCAGCGAAGTGATGGTCGCAGAGAACAAAAGTGGAAAGAGAAGGCGAAGCTTGACAGAACTTCTACAGAGTCAGAGGCTGCTGCTGAAGTTGTCACTAATGCACTTGGTAAATTGAGAGTCAGTGAAAGTGGTCAACCTCATGTTCCTATTTCTAGTAGGGAGTTTGGAAATGCGCAGCTAACAAACCAGGTCCCCTCTGGGCTTGGAAATAGAGGGATTTGGAAACCGAAAGCATATGGAACAACCAGTGGGGGGGCAGCAGTGGTTGAAGCTGAAAAGGCACCTGCCGTTGGAACATCTATTGAAAACAAGGGGAACACTGCTGGACTAGCTGCACAGAATGGCACTGTTGGCTTGAGTCAACTATTCAAGGGCAATCAGATAGAAAATTTTACTGTGGACAACTCCACTTACACACAGGCACAAATAAGAGCTACGTTCTATCCTAAATTTGAGAACGAGAAGTCAGATCAGGAGATTAGAACAAGGATGATAGAGATGGTATCGAAAGGCTTGGCAACATTGGAGGTTTCACTTAAACACTCAGGGTCTTTGTTTATGTACGCTGGCCATGAAGGTGGAGCATACGCCAAAAACAGCTTCGGGAATATATACACTGCTGTTGGTGTTTTTGTTCTGGGAAGGATGTTTCGAGAGGCCTGGGGATCTAAAGCAGCAAAAAAGCAAGCAGAATTCAATAATTTTCTTGAGATCAATTTCTGTGAAATCCCTCCTAATGATAAGTTCACTTGGTCTAGCTTTAGGGAGGATGTAGTTTGGTCTTTAACGAAACTGGAGATCATGGGAACCATGTCGTTTAGGTTTGTTTTCTTCACTCTTTTGAAGCTTGGTGGTAGGACACCAAATGGAAGGATGGCCCAGCTTTCTCTTTCCCTCAGAAAGTCATAA

Coding sequence (CDS)

ATGTCGGCGTCGCACAGGATCTTCTGCGCCATCACTCTCCCTCACCCTCCTCGTTTCTCGCCCTCTTCACTCTTCAATTCCAGGGCCTTCCTCTCCACTTCCCACTTCATTTTTCCCCGCTCTCTCGCCCTCCCGCCTCTAATTTCTTCCCCTTTTCACCTTTCTCCTCATTCTCGATCGATCATGCCTTACAATCAGCTTATTGAGTTTGATCTTAATTTGCAGCGAAGTGATGGTCGCAGAGAACAAAAGTGGAAAGAGAAGGCGAAGCTTGACAGAACTTCTACAGAGTCAGAGGCTGCTGCTGAAGTTGTCACTAATGCACTTGGTAAATTGAGAGTCAGTGAAAGTGGTCAACCTCATGTTCCTATTTCTAGTAGGGAGTTTGGAAATGCGCAGCTAACAAACCAGGTCCCCTCTGGGCTTGGAAATAGAGGGATTTGGAAACCGAAAGCATATGGAACAACCAGTGGGGGGGCAGCAGTGGTTGAAGCTGAAAAGGCACCTGCCGTTGGAACATCTATTGAAAACAAGGGGAACACTGCTGGACTAGCTGCACAGAATGGCACTGTTGGCTTGAGTCAACTATTCAAGGGCAATCAGATAGAAAATTTTACTGTGGACAACTCCACTTACACACAGGCACAAATAAGAGCTACGTTCTATCCTAAATTTGAGAACGAGAAGTCAGATCAGGAGATTAGAACAAGGATGATAGAGATGGTATCGAAAGGCTTGGCAACATTGGAGGTTTCACTTAAACACTCAGGGTCTTTGTTTATGTACGCTGGCCATGAAGGTGGAGCATACGCCAAAAACAGCTTCGGGAATATATACACTGCTGTTGGTGTTTTTGTTCTGGGAAGGATGTTTCGAGAGGCCTGGGGATCTAAAGCAGCAAAAAAGCAAGCAGAATTCAATAATTTTCTTGAGATCAATTTCTGTGAAATCCCTCCTAATGATAAGTTCACTTGGTCTAGCTTTAGGGAGGATGTAGTTTGGTCTTTAACGAAACTGGAGATCATGGGAACCATGTCGTTTAGGTTTGTTTTCTTCACTCTTTTGAAGCTTGGTGGTAGGACACCAAATGGAAGGATGGCCCAGCTTTCTCTTTCCCTCAGAAAGTCATAA

Protein sequence

MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRSIMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNTAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNNFLEINFCEIPPNDKFTWSSFREDVVWSLTKLEIMGTMSFRFVFFTLLKLGGRTPNGRMAQLSLSLRKS

Homology

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BLAST of MC03g_new0203 vs. ExPASy Swiss-Prot
Match: Q0WL81 (tRNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=RNL PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.4e-61
Identity = 134/226 (59.29%), Postives = 157/226 (69.47%), Query Frame = 0


Query: 89  AKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSREFGNAQLTNQVPSGLGNRGIW 148
           A  +   + +   AE V N  G L + ES      + S+   N ++ N V         W
Sbjct: 3   APFESGDSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQNLV---------W 62

Query: 149 KPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNT-AGLAAQNGTVGLSQLFKGNQIENFTV 208
           KPK+YGT SG ++  E  K  AV + I + G+T  GL        LS++F GN +E F+V
Sbjct: 63  KPKSYGTVSGSSSATEVGKTSAV-SQIGSSGDTKVGL-------NLSKIFGGNLLEKFSV 122

Query: 209 DNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHEG 268
           D STY  AQIRATFYPKFENEK+DQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGH+G
Sbjct: 123 DKSTYCHAQIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKG 182

Query: 269 GAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNNFLEIN 314
           GAYAKNSFGNIYTAVGVFVL RMFREAWG+KA KK+AEFN+FLE N
Sbjct: 183 GAYAKNSFGNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKN 211

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BLAST of MC03g_new0203 vs. NCBI nr
Match: XP_022157928.1 (tRNA ligase 1 [Momordica charantia])

HSP 1 Score: 586 bits (1511), Expect = 3.87e-197
Identity = 303/313 (96.81%), Postives = 303/313 (96.81%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60
           MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS
Sbjct: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60

Query: 61  IMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP 120
           IMPYNQ         RSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP
Sbjct: 61  IMPYNQ---------RSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP 120

Query: 121 HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN 180
           HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN
Sbjct: 121 HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN 180

Query: 181 TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240
           TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE
Sbjct: 181 TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240

Query: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA 300
           MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA
Sbjct: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA 300

Query: 301 KKQAEFNNFLEIN 313
           KKQAEFNNFLE N
Sbjct: 301 KKQAEFNNFLESN 304

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BLAST of MC03g_new0203 vs. NCBI nr
Match: XP_038894223.1 (tRNA ligase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 461 bits (1185), Expect = 1.09e-148
Identity = 251/317 (79.18%), Postives = 265/317 (83.60%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFL----STSHFIFPRSLALPPLISSPFHLSP 60
           MSAS RIFCAITLPHP  ++PS+ FN RAF       SHFI PRSL L PL SSPF LS 
Sbjct: 1   MSASQRIFCAITLPHPRLYAPSA-FNYRAFPFICHPLSHFILPRSLTLAPLTSSPFPLSR 60

Query: 61  HSRSIMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSE 120
            SR IMPYNQ         R  GRREQKWKEKAK+DR STESEAAAEVVTNALGKLRV+E
Sbjct: 61  DSRFIMPYNQ---------RKGGRREQKWKEKAKVDRNSTESEAAAEVVTNALGKLRVTE 120

Query: 121 SGQPHVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIE 180
           + QPHV  SS +FGNAQLTNQV  GL +R +WKPKAYGTTSG AA VE EKAP  GTS E
Sbjct: 121 NDQPHVLTSSAQFGNAQLTNQVTPGLAHRAVWKPKAYGTTSG-AAEVEGEKAPTNGTSTE 180

Query: 181 NKGNTAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRT 240
           NKG+ A LAAQNG VGLSQLFKGNQIE FTVDNSTYT+AQIRATFYPKFENEKSDQEIRT
Sbjct: 181 NKGSNAELAAQNGAVGLSQLFKGNQIEKFTVDNSTYTRAQIRATFYPKFENEKSDQEIRT 240

Query: 241 RMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWG 300
           RMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMF+EAWG
Sbjct: 241 RMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFQEAWG 300

Query: 301 SKAAKKQAEFNNFLEIN 313
           + AAKKQAEFN+FLE N
Sbjct: 301 AAAAKKQAEFNDFLESN 306

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BLAST of MC03g_new0203 vs. NCBI nr
Match: XP_004147268.2 (tRNA ligase 1 isoform X1 [Cucumis sativus] >KGN64758.2 hypothetical protein Csa_013879 [Cucumis sativus])

HSP 1 Score: 437 bits (1125), Expect = 6.95e-140
Identity = 240/315 (76.19%), Postives = 255/315 (80.95%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLS--TSHFIFPRSLALPPLISSPFHLSPHS 60
           MSA  RIFCA TLPHPP    SS +    F+S   SH+I PRSL L PL SSP  +S  S
Sbjct: 1   MSALQRIFCAKTLPHPPF---SSSYRVFPFISHPLSHYILPRSLTLAPLTSSPLPISCDS 60

Query: 61  RSIMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESG 120
           R +MPYNQ         R   R EQKWKEKAK DR STESEAAAEVVTNALGKLRV+ES 
Sbjct: 61  RFVMPYNQ---------RRGSRGEQKWKEKAKADRNSTESEAAAEVVTNALGKLRVTESD 120

Query: 121 QPHVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENK 180
           QPHV  SS +FGNAQLTNQ   GL +R IWKPKAYGTTSG AAV+E EKAP   TS ENK
Sbjct: 121 QPHVLTSSAQFGNAQLTNQATPGLAHRAIWKPKAYGTTSG-AAVIEGEKAPTNETSTENK 180

Query: 181 GNTAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRM 240
           G+ AG+AAQ+G V LSQLFK NQIE FTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRM
Sbjct: 181 GSNAGVAAQDGVVSLSQLFKSNQIEKFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRM 240

Query: 241 IEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSK 300
           IEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWG++
Sbjct: 241 IEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGAE 300

Query: 301 AAKKQAEFNNFLEIN 313
           AAKKQAEFN+FLE N
Sbjct: 301 AAKKQAEFNDFLESN 302

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BLAST of MC03g_new0203 vs. NCBI nr
Match: XP_008463605.1 (PREDICTED: uncharacterized protein LOC103501711 isoform X1 [Cucumis melo])

HSP 1 Score: 427 bits (1097), Expect = 8.94e-136
Identity = 235/313 (75.08%), Postives = 249/313 (79.55%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60
           MSA  RIF A  LPHPP  S   +F        SHFI PRSL L PL SSPF LS  SR 
Sbjct: 1   MSALQRIFYAKILPHPPFSSSYKVFPFICH-PLSHFILPRSLTLAPLTSSPFPLSCDSRF 60

Query: 61  IMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP 120
           +MPYNQ         R  GR EQKWKEKAK+D++ TESEAA EVVTNALGKLRV+ES Q 
Sbjct: 61  VMPYNQ---------RRGGRGEQKWKEKAKVDKSPTESEAAVEVVTNALGKLRVTESDQS 120

Query: 121 HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN 180
           HV  SS +FGNAQLTNQ   GL +R IWKPKAYGTTSG AAV+E EKA   GTS ENKG+
Sbjct: 121 HVLTSSAQFGNAQLTNQAIPGLAHRAIWKPKAYGTTSG-AAVIEGEKASTNGTSTENKGS 180

Query: 181 TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240
            AGLA Q G VGLSQLFK NQIE F VDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE
Sbjct: 181 NAGLAVQGGAVGLSQLFKSNQIEKFIVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240

Query: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA 300
           MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWG++AA
Sbjct: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGAEAA 300

Query: 301 KKQAEFNNFLEIN 313
           KKQAEFN+FL+ N
Sbjct: 301 KKQAEFNDFLQSN 302

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BLAST of MC03g_new0203 vs. NCBI nr
Match: XP_023519581.1 (tRNA ligase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519582.1 tRNA ligase 1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422 bits (1086), Expect = 3.95e-134
Identity = 236/318 (74.21%), Postives = 256/318 (80.50%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAF----LSTSHFIFPRSLA-LPPLISSPFHLS 60
           MSA HRIF AITLP P RFS SS F+ RAF     S SHFI P SL  L   I+ PF + 
Sbjct: 1   MSAPHRIFRAITLPLP-RFSSSSTFHYRAFPFIPCSLSHFILPPSLLILSTSIAFPFSVL 60

Query: 61  PHSRSIMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVS 120
            +SR +MPYNQ         R  GRREQKWKEKAK++  STESEAA+EVVTNAL  LRV+
Sbjct: 61  WNSRFLMPYNQ---------RRGGRREQKWKEKAKVEGISTESEAASEVVTNALSNLRVT 120

Query: 121 ESGQPHVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSI 180
           ES QPH+PI+S +FGNAQ TN    GLG+R IWKPKAYGTTSG AAVVE EKAPAVGTSI
Sbjct: 121 ESNQPHIPITSVQFGNAQPTNLATPGLGHRAIWKPKAYGTTSG-AAVVEGEKAPAVGTSI 180

Query: 181 ENKGNTAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIR 240
           ENKG+ A +AA +  + L+QL KGNQIE FTVDNS YTQAQIRATFYPKFENEKSDQEIR
Sbjct: 181 ENKGSNAEIAANSSAIPLTQLLKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIR 240

Query: 241 TRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAW 300
           TRMIEMVSKGLATLEVSLKHSGSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAW
Sbjct: 241 TRMIEMVSKGLATLEVSLKHSGSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAW 300

Query: 301 GSKAAKKQAEFNNFLEIN 313
           GS A KKQAEFN+FLE N
Sbjct: 301 GSVAPKKQAEFNDFLESN 307

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BLAST of MC03g_new0203 vs. ExPASy TrEMBL
Match: A0A6J1DUP6 (tRNA ligase 1 OS=Momordica charantia OX=3673 GN=LOC111024537 PE=4 SV=1)

HSP 1 Score: 586 bits (1511), Expect = 1.88e-197
Identity = 303/313 (96.81%), Postives = 303/313 (96.81%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60
           MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS
Sbjct: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60

Query: 61  IMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP 120
           IMPYNQ         RSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP
Sbjct: 61  IMPYNQ---------RSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP 120

Query: 121 HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN 180
           HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN
Sbjct: 121 HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN 180

Query: 181 TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240
           TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE
Sbjct: 181 TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240

Query: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA 300
           MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA
Sbjct: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA 300

Query: 301 KKQAEFNNFLEIN 313
           KKQAEFNNFLE N
Sbjct: 301 KKQAEFNNFLESN 304

[Close]

BLAST of MC03g_new0203 vs. ExPASy TrEMBL
Match: A0A1S3CK49 (uncharacterized protein LOC103501711 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501711 PE=4 SV=1)

HSP 1 Score: 427 bits (1097), Expect = 4.33e-136
Identity = 235/313 (75.08%), Postives = 249/313 (79.55%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60
           MSA  RIF A  LPHPP  S   +F        SHFI PRSL L PL SSPF LS  SR 
Sbjct: 1   MSALQRIFYAKILPHPPFSSSYKVFPFICH-PLSHFILPRSLTLAPLTSSPFPLSCDSRF 60

Query: 61  IMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQP 120
           +MPYNQ         R  GR EQKWKEKAK+D++ TESEAA EVVTNALGKLRV+ES Q 
Sbjct: 61  VMPYNQ---------RRGGRGEQKWKEKAKVDKSPTESEAAVEVVTNALGKLRVTESDQS 120

Query: 121 HVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGN 180
           HV  SS +FGNAQLTNQ   GL +R IWKPKAYGTTSG AAV+E EKA   GTS ENKG+
Sbjct: 121 HVLTSSAQFGNAQLTNQAIPGLAHRAIWKPKAYGTTSG-AAVIEGEKASTNGTSTENKGS 180

Query: 181 TAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240
            AGLA Q G VGLSQLFK NQIE F VDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE
Sbjct: 181 NAGLAVQGGAVGLSQLFKSNQIEKFIVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIE 240

Query: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAA 300
           MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWG++AA
Sbjct: 241 MVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGAEAA 300

Query: 301 KKQAEFNNFLEIN 313
           KKQAEFN+FL+ N
Sbjct: 301 KKQAEFNDFLQSN 302

[Close]

BLAST of MC03g_new0203 vs. ExPASy TrEMBL
Match: A0A5A7UUA9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold778G00320 PE=4 SV=1)

HSP 1 Score: 414 bits (1063), Expect = 8.58e-133
Identity = 238/357 (66.67%), Postives = 256/357 (71.71%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFLSTSHFIFPRSLALPPLISSPFHLSPHSRS 60
           MSA  RIF A  LPHPP  S   +F        SHFI PRSL L PL SSPF LS  SR 
Sbjct: 1   MSALQRIFYAKILPHPPFSSSYKVFPFICH-PLSHFILPRSLTLAPLTSSPFPLSCDSRF 60

Query: 61  IMPYNQ--LIEFD-------------------------------LNL-----------QR 120
           +MPYNQ  ++ FD                               +N+            R
Sbjct: 61  VMPYNQVPIVLFDEFGCKFTLFSVDVEDGRGLILSFLIYFQRFVINVFTMLARKKDAAMR 120

Query: 121 SDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSREFGNAQLTN 180
             GR EQKWKEKAK+D++ TESEAA EVVTNALGKLRV+ES Q HV  SS +FGNAQLTN
Sbjct: 121 RGGRGEQKWKEKAKVDKSPTESEAAVEVVTNALGKLRVTESDQSHVLTSSAQFGNAQLTN 180

Query: 181 QVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNTAGLAAQNGTVGLSQL 240
           Q   GL +R IWKPKAYGTTSG AAV+E EKA   GTS ENKG+ AGLA Q G VGLSQL
Sbjct: 181 QAIPGLAHRAIWKPKAYGTTSG-AAVIEGEKASTNGTSTENKGSNAGLAVQGGAVGLSQL 240

Query: 241 FKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS 300
           FK NQIE F VDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS
Sbjct: 241 FKSNQIEKFIVDNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHS 300

Query: 301 GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNNFLEIN 313
           GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWG++AAKKQAEFN+FL+ N
Sbjct: 301 GSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWGAEAAKKQAEFNDFLQSN 355

[Close]

BLAST of MC03g_new0203 vs. ExPASy TrEMBL
Match: A0A6J1HM92 (tRNA ligase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464863 PE=4 SV=1)

HSP 1 Score: 415 bits (1067), Expect = 1.05e-131
Identity = 231/317 (72.87%), Postives = 250/317 (78.86%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAF----LSTSHFIFPRSLALPPLISSPFHLSP 60
           MSA++RIFCAITLP     S S   +SRAF     S SHFI   SL LP  +  PF +  
Sbjct: 1   MSATYRIFCAITLP----LSSSPALHSRAFPFVSCSLSHFILHPSLTLPASVF-PFTVCR 60

Query: 61  HSRSIMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVSE 120
            SR  MPYNQ         R  GRREQKWKEKAK++  STESE A+EVVTNAL  LRV+E
Sbjct: 61  DSRFTMPYNQ---------RRGGRREQKWKEKAKVEGISTESETASEVVTNALSNLRVTE 120

Query: 121 SGQPHVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSIE 180
           S QPH+PI+S +FGNAQ TN    GLG+R IWKPKAYGTTSG AAVVE EKAPAVGTSIE
Sbjct: 121 SNQPHIPITSVQFGNAQPTNLATPGLGHRAIWKPKAYGTTSG-AAVVEGEKAPAVGTSIE 180

Query: 181 NKGNTAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIRT 240
           NKG+ A +AA +  + LSQL KGNQIE FTVDNS YTQAQIRATFYPKFENEKSDQEIRT
Sbjct: 181 NKGSNAEIAANSSAIALSQLLKGNQIEQFTVDNSAYTQAQIRATFYPKFENEKSDQEIRT 240

Query: 241 RMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAWG 300
           RMIEMVSKGLATLEVSLKHSGSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMFREAWG
Sbjct: 241 RMIEMVSKGLATLEVSLKHSGSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFREAWG 300

Query: 301 SKAAKKQAEFNNFLEIN 313
           S A KKQAEFN+FLE N
Sbjct: 301 SVAPKKQAEFNDFLESN 302

[Close]

BLAST of MC03g_new0203 vs. ExPASy TrEMBL
Match: A0A6J1I3R5 (tRNA ligase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469400 PE=4 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 1.26e-129
Identity = 228/318 (71.70%), Postives = 248/318 (77.99%), Query Frame = 0


Query: 1   MSASHRIFCAITLPHPPRFSPSSLFNSRAFL-----STSHFIFPRSLALPPLISSPFHLS 60
           MSA HRIFCAITLP   R S SS FN R F      S SH I   SL +   IS P  +S
Sbjct: 1   MSAPHRIFCAITLPRH-RLSYSSAFNYRVFFPFIPYSFSHRILSPSLTITDSISFPSTVS 60

Query: 61  PHSRSIMPYNQLIEFDLNLQRSDGRREQKWKEKAKLDRTSTESEAAAEVVTNALGKLRVS 120
              R +MPYNQ         R  GRREQKWKEKAK++  STESEAA++VVTNAL  LRV+
Sbjct: 61  SDFRFMMPYNQ---------RRGGRREQKWKEKAKVEGISTESEAASQVVTNALSNLRVT 120

Query: 121 ESGQPHVPISSREFGNAQLTNQVPSGLGNRGIWKPKAYGTTSGGAAVVEAEKAPAVGTSI 180
           ES QPH+PI+S +FGNAQ TN    GLG+R IWKPKAYGTT G AAVVE EKA AVGTSI
Sbjct: 121 ESNQPHIPITSVQFGNAQPTNLATPGLGHRAIWKPKAYGTTIG-AAVVEGEKASAVGTSI 180

Query: 181 ENKGNTAGLAAQNGTVGLSQLFKGNQIENFTVDNSTYTQAQIRATFYPKFENEKSDQEIR 240
           ENKG+ A +AA +  + L+QL KGNQIE FTVDNS YTQAQIRATFYPKFENEKSDQEIR
Sbjct: 181 ENKGSNAEIAANSSAIALNQLLKGNQIEKFTVDNSAYTQAQIRATFYPKFENEKSDQEIR 240

Query: 241 TRMIEMVSKGLATLEVSLKHSGSLFMYAGHEGGAYAKNSFGNIYTAVGVFVLGRMFREAW 300
           TRMIEMVSKGLATLEVSLKHSGSLFMYAGH+GGAYAKNSFGNIYTAVGVFVLGRMF+EAW
Sbjct: 241 TRMIEMVSKGLATLEVSLKHSGSLFMYAGHQGGAYAKNSFGNIYTAVGVFVLGRMFQEAW 300

Query: 301 GSKAAKKQAEFNNFLEIN 313
           GS A KKQAEFN+FLE N
Sbjct: 301 GSVAPKKQAEFNDFLESN 307

[Close]

BLAST of MC03g_new0203 vs. TAIR 10
Match: AT1G07910.1 (RNAligase )

HSP 1 Score: 236.1 bits (601), Expect = 4.6e-62
Identity = 134/226 (59.29%), Postives = 157/226 (69.47%), Query Frame = 0


Query: 89  AKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSREFGNAQLTNQVPSGLGNRGIW 148
           A  +   + +   AE V N  G L + ES      + S+   N ++ N V         W
Sbjct: 3   APFESGDSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQNLV---------W 62

Query: 149 KPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNT-AGLAAQNGTVGLSQLFKGNQIENFTV 208
           KPK+YGT SG ++  E  K  AV + I + G+T  GL        LS++F GN +E F+V
Sbjct: 63  KPKSYGTVSGSSSATEVGKTSAV-SQIGSSGDTKVGL-------NLSKIFGGNLLEKFSV 122

Query: 209 DNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHEG 268
           D STY  AQIRATFYPKFENEK+DQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGH+G
Sbjct: 123 DKSTYCHAQIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKG 182

Query: 269 GAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNNFLEIN 314
           GAYAKNSFGNIYTAVGVFVL RMFREAWG+KA KK+AEFN+FLE N
Sbjct: 183 GAYAKNSFGNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKN 211

[Close]

BLAST of MC03g_new0203 vs. TAIR 10
Match: AT1G07910.2 (RNAligase )

HSP 1 Score: 236.1 bits (601), Expect = 4.6e-62
Identity = 134/226 (59.29%), Postives = 157/226 (69.47%), Query Frame = 0


Query: 89  AKLDRTSTESEAAAEVVTNALGKLRVSESGQPHVPISSREFGNAQLTNQVPSGLGNRGIW 148
           A  +   + +   AE V N  G L + ES      + S+   N ++ N V         W
Sbjct: 3   APFESGDSSATVVAEAVNNQFGGLSLKESNTNAPVLPSQTTSNHRVQNLV---------W 62

Query: 149 KPKAYGTTSGGAAVVEAEKAPAVGTSIENKGNT-AGLAAQNGTVGLSQLFKGNQIENFTV 208
           KPK+YGT SG ++  E  K  AV + I + G+T  GL        LS++F GN +E F+V
Sbjct: 63  KPKSYGTVSGSSSATEVGKTSAV-SQIGSSGDTKVGL-------NLSKIFGGNLLEKFSV 122

Query: 209 DNSTYTQAQIRATFYPKFENEKSDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHEG 268
           D STY  AQIRATFYPKFENEK+DQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGH+G
Sbjct: 123 DKSTYCHAQIRATFYPKFENEKTDQEIRTRMIEMVSKGLATLEVSLKHSGSLFMYAGHKG 182

Query: 269 GAYAKNSFGNIYTAVGVFVLGRMFREAWGSKAAKKQAEFNNFLEIN 314
           GAYAKNSFGNIYTAVGVFVL RMFREAWG+KA KK+AEFN+FLE N
Sbjct: 183 GAYAKNSFGNIYTAVGVFVLSRMFREAWGTKAPKKEAEFNDFLEKN 211

The following BLAST results are available for this feature:

BLAST of MC03g_new0203 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WL81	6.4e-61	59.29	tRNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=RNL PE=1 SV=1	[more]

BLAST of MC03g_new0203 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157928.1	3.87e-197	96.81	tRNA ligase 1 [Momordica charantia]	[more]
XP_038894223.1	1.09e-148	79.18	tRNA ligase 1 isoform X1 [Benincasa hispida]	[more]
XP_004147268.2	6.95e-140	76.19	tRNA ligase 1 isoform X1 [Cucumis sativus] >KGN64758.2 hypothetical protein Csa_...	[more]
XP_008463605.1	8.94e-136	75.08	PREDICTED: uncharacterized protein LOC103501711 isoform X1 [Cucumis melo]	[more]
XP_023519581.1	3.95e-134	74.21	tRNA ligase 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519582.1 tRNA ligas...	[more]

BLAST of MC03g_new0203 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUP6	1.88e-197	96.81	tRNA ligase 1 OS=Momordica charantia OX=3673 GN=LOC111024537 PE=4 SV=1	[more]
A0A1S3CK49	4.33e-136	75.08	uncharacterized protein LOC103501711 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A5A7UUA9	8.58e-133	66.67	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A6J1HM92	1.05e-131	72.87	tRNA ligase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464863 PE=4 SV=1	[more]
A0A6J1I3R5	1.26e-129	71.70	tRNA ligase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469400 PE=4 SV=1	[more]

BLAST of MC03g_new0203 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G07910.1	4.6e-62	59.29	RNAligase	[more]
AT1G07910.2	4.6e-62	59.29	RNAligase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR35460:SF1	TRNA LIGASE 1	coord: 145..313
IPR038837	tRNA ligase 1	PANTHER	PTHR35460	TRNA LIGASE 1	coord: 145..313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0203.1	MC03g_new0203.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003972	RNA ligase (ATP) activity