MC03g_new0106 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g_new0106
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ribonuclease H
Location	MC03: 5026851 .. 5029351 (-)
RNA-Seq Expression	MC03g_new0106
Synteny	MC03g_new0106

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGAGTGCGAGCAAGCGTTTCAGCAATTGAAGAGCTACCTCTGTTCGGCACCCTTGCTTGCCAAGCCCATGCCGGGGGACAAGCTCCAATTGTACCTAGCAGTGTCTGACAGTGCCGTCAGCTCGGCCCTAATCAGGCAAGAGGAAGCGCGGCAAAACCCGGTCTACTACACAAGCAAGGCTATGACCGAAGCCGAGACTAGATACCCTCAGATGGAGAAGTTGGCTCTCGCTTTGGTCACCTCGGCCCGACGACTTAGACCATACTTCCAAGCCCATACGGTGGTGGTGCTCACTAACTTGCCCCTTAAAAGTATCTTCCACAAGCCGGAAGCTTCCGGACGCCTAATGAAGTGGGCAATAGAGCTAAGTGAGTACGACATCCAGTTCGAACCTAGAACTGCGTTGAAAGGACAAGCAGCGGCAGATTTCATAGCCGAGCTCACACCACCTTCCGAGCTGAGCGAGTCCGACCTACCTTGGACAGTCTATGTCGACGGATCCTCCAATGAGAAGGGGTGCGGAGCCGGGGTCCTCTTGCTCGGACCAGGGGGTGAGCGATTTGAGTATGCCTTGCGGTTCAGCTTCCGGACTTCTAACAACGAGGCTGAGTATGAAGCTTTTATTGCCGGCCTGCGAATCGCTCGAACATTGGGGGCCTCTTGTATTAAGGTCTTCAGTGACTCCCAGCTGGTTGTGAGCCAGATCAAGGACGAATACCAAGCCAAAGACACCCGAATGGAGAAGTATTTGGGCAAGGTCAGATCATACCTCGCCCAGTTTCGAACTTACGAGGTAAGCCGGATTCCGCGAGCAGAAAATTCTAATGCTGACGCCTTGGCCAAGTTAGCATCGGCGTATGAGACCGACCTCGCCAGGTCGGTCCCCGTCGAGATCCTAGATAATCCCTCGATCTTAGAACCAGATCTGATGGAGGTCGGCGCTCCAGAATCCTCATGGATGGACCCGATCACGGACTTCATTAGGGGTAACTCACCACAAGATCCCAAGGAGCGCAGAAAGTTGGCAAGGCGGACAGCTCGGCTCGTGGTCCGAGATGGTGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAAATGCCTAACCCCTGAAGAGGGCCTGTACGTCCTCAGAGAGATCCACGAAGGAGTGTGCGGCAATCACTCAGGCGCCCGGTCGCTGTCAGCCAAAGTGATCCGGCAAGGATACTATTGGCCGACCCTCAGCCAGGACGCCAAGAAGTTCGTTAGAACTTGCGACAATTGCCAACGCTACGGAACCATAATCCGCCAACCTCCCGAGCTGCTCACCCCCATCTCGGCCCCATGGCCATTCGCGCAGTGGGGGGTAGATATCATTGGTCCTTTCCCTTTGGGCAAGGGCCAGACCAAGTTCGCTGTGGTTGCTGTGGATTACTTCACCAAGTGGGCCGAGGCCGAGGCGCTCTCCCACATAACGGAATCCAGGGTCACGTCCTTCGTATGGACGAATATCATATGTCGCTTTGGTATACCGCAGGCCATAGTGACAGACAATGGGAAGCAGTTTGACAACGCCAAGTTCAAAGACTTTTGCAGCAAACTTGGCATAAGTCATCTCAGCTCGTCCCCCGCACACCCGCAAGCAAATGGGCAGGTGGAGGCAGTCAACAAGATCATCAAGCGAGGCATCAAACTTAGACTGGACTCCAAGAAAGGCAGGTGGGCCGAGGAGCTACTAGAGGTTCTGTGGTCGTACCGGACCACCCAACGAGAGTCGACGGGTGAGACCCCGTTCTCCCTGGCCTTCGGCTCCGAAGCTGTAGTCCCGGTTGAGATCGGCATGCCATCTGACAGAGTAGAGCATTACGACCCTACGACGAATGAGGATGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGGAGAGCAATGGCCCAGCTACGCCTGGCGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAGCGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCGTTTGAGATTAAGGGCATAATCCGACCTGGGACGTACATATTGGCCGATCTGAAAGGTGACGTCCTCGCGCACCCGTGGAACGCGGAGCACCTGAAGCGTTATTATCCTTGAAATGACAAAAGGATTTTCAATGCATCTGCAAAGACTGTTCCGAAAGAATTATGATCGGAATAAATGTGATGATTTAATTTCTTGCTTCCGAGCTCGACCAGAAATTAAATTGGGGCCACGGACTCCTACGGGATCGCATTCCAGCAGTTGGTTCAAATTCAATCCTTCGAAGCCTAAGGGTACGAGGTGCGATGCCAAAACCACTGACGAACTTAAAATACAATAAAAATATGACAAAGAAATAAACTAAAGTGAAATACTAATATGATTTAAAGAAGAATAAGAAAACATCAGATCTCTTCAAGGTAGTAAAGTATTTTATCCGCT

mRNA sequence

ATGCCGGGGGACAAGCTCCAATTGTACCTAGCAGTGTCTGACAGTGCCGTCAGCTCGGCCCTAATCAGGCAAGAGGAAGCGCGGCAAAACCCGGTCTACTACACAAGCAAGGCTATGACCGAAGCCGAGACTAGATACCCTCAGATGGAGAAGTTGGCTCTCGCTTTGGTCACCTCGGCCCGACGACTTAGACCATACTTCCAAGCCCATACGGTGGTGGTGCTCACTAACTTGCCCCTTAAAAGTATCTTCCACAAGCCGGAAGCTTCCGGACGCCTAATGAAGTGGGCAATAGAGCTAAGTGAGTACGACATCCAGTTCGAACCTAGAACTGCGTTGAAAGGACAAGCAGCGGCAGATTTCATAGCCGAGCTCACACCACCTTCCGAGCTGAGCGAGTCCGACCTACCTTGGACAGTCTATGTCGACGGATCCTCCAATGAGAAGGGGTGCGGAGCCGGGGTCCTCTTGCTCGGACCAGGGGGTGAGCGATTTGAGTATGCCTTGCGGTTCAGCTTCCGGACTTCTAACAACGAGGCTGAGTATGAAGCTTTTATTGCCGGCCTGCGAATCGCTCGAACATTGGGGGCCTCTTGTATTAAGGTCTTCAGTGACTCCCAGCTGGTTGTGAGCCAGATCAAGGACGAATACCAAGCCAAAGACACCCGAATGGAGAAGTATTTGGGCAAGGTCAGATCATACCTCGCCCAGTTTCGAACTTACGAGGTAAGCCGGATTCCGCGAGCAGAAAATTCTAATGCTGACGCCTTGGCCAAGTTAGCATCGGCGTATGAGACCGACCTCGCCAGGTCGGTCCCCGTCGAGATCCTAGATAATCCCTCGATCTTAGAACCAGATCTGATGGAGGTCGGCGCTCCAGAATCCTCATGGATGGACCCGATCACGGACTTCATTAGGGGTAACTCACCACAAGATCCCAAGGAGCGCAGAAAGTTGGCAAGGCGGACAGCTCGGCTCGTGGTCCGAGATGGTGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAAATGCCTAACCCCTGAAGAGGGCCTGTACGTCCTCAGAGAGATCCACGAAGGAGTGTGCGGCAATCACTCAGGCGCCCGGTCGCTGTCAGCCAAAGTGATCCGGCAAGGATACTATTGGCCGACCCTCAGCCAGGACGCCAAGAAGTTCGTTAGAACTTGCGACAATTGCCAACGCTACGGAACCATAATCCGCCAACCTCCCGAGCTGCTCACCCCCATCTCGGCCCCATGGCCATTCGCGCAGTGGGGGGTAGATATCATTGGTCCTTTCCCTTTGGGCAAGGGCCAGACCAAGTTCGCTGTGGTTGCTGTGGATTACTTCACCAAGTGGGCCGAGGCCGAGGCGCTCTCCCACATAACGGAATCCAGGGTCACGTCCTTCGTATGGACGAATATCATATGTCGCTTTGGTATACCGCAGGCCATAGTGACAGACAATGGGAAGCAGTTTGACAACGCCAAGTTCAAAGACTTTTGCAGCAAACTTGGCATAAGTCATCTCAGCTCGTCCCCCGCACACCCGCAAGCAAATGGGCAGGTGGAGGCAGTCAACAAGATCATCAAGCGAGGCATCAAACTTAGACTGGACTCCAAGAAAGGCAGGTGGGCCGAGGAGCTACTAGAGGTTCTGTGGTCGTACCGGACCACCCAACGAGAGTCGACGGGTGAGACCCCGTTCTCCCTGGCCTTCGGCTCCGAAGCTGTAGTCCCGGTTGAGATCGGCATGCCATCTGACAGAGTAGAGCATTACGACCCTACGACGAATGAGGATGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGGAGAGCAATGGCCCAGCTACGCCTGGCGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAGCGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCGTTTGAGATTAAGGGCATAATCCGACCTGGGACGTACATATTGGCCGATCTGAAAGGTGACGTCCTCGCGCACCCGTGGAACGCGGAGCACCTGAAGCGTTATTATCCTTGA

Coding sequence (CDS)

ATGCCGGGGGACAAGCTCCAATTGTACCTAGCAGTGTCTGACAGTGCCGTCAGCTCGGCCCTAATCAGGCAAGAGGAAGCGCGGCAAAACCCGGTCTACTACACAAGCAAGGCTATGACCGAAGCCGAGACTAGATACCCTCAGATGGAGAAGTTGGCTCTCGCTTTGGTCACCTCGGCCCGACGACTTAGACCATACTTCCAAGCCCATACGGTGGTGGTGCTCACTAACTTGCCCCTTAAAAGTATCTTCCACAAGCCGGAAGCTTCCGGACGCCTAATGAAGTGGGCAATAGAGCTAAGTGAGTACGACATCCAGTTCGAACCTAGAACTGCGTTGAAAGGACAAGCAGCGGCAGATTTCATAGCCGAGCTCACACCACCTTCCGAGCTGAGCGAGTCCGACCTACCTTGGACAGTCTATGTCGACGGATCCTCCAATGAGAAGGGGTGCGGAGCCGGGGTCCTCTTGCTCGGACCAGGGGGTGAGCGATTTGAGTATGCCTTGCGGTTCAGCTTCCGGACTTCTAACAACGAGGCTGAGTATGAAGCTTTTATTGCCGGCCTGCGAATCGCTCGAACATTGGGGGCCTCTTGTATTAAGGTCTTCAGTGACTCCCAGCTGGTTGTGAGCCAGATCAAGGACGAATACCAAGCCAAAGACACCCGAATGGAGAAGTATTTGGGCAAGGTCAGATCATACCTCGCCCAGTTTCGAACTTACGAGGTAAGCCGGATTCCGCGAGCAGAAAATTCTAATGCTGACGCCTTGGCCAAGTTAGCATCGGCGTATGAGACCGACCTCGCCAGGTCGGTCCCCGTCGAGATCCTAGATAATCCCTCGATCTTAGAACCAGATCTGATGGAGGTCGGCGCTCCAGAATCCTCATGGATGGACCCGATCACGGACTTCATTAGGGGTAACTCACCACAAGATCCCAAGGAGCGCAGAAAGTTGGCAAGGCGGACAGCTCGGCTCGTGGTCCGAGATGGTGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAAATGCCTAACCCCTGAAGAGGGCCTGTACGTCCTCAGAGAGATCCACGAAGGAGTGTGCGGCAATCACTCAGGCGCCCGGTCGCTGTCAGCCAAAGTGATCCGGCAAGGATACTATTGGCCGACCCTCAGCCAGGACGCCAAGAAGTTCGTTAGAACTTGCGACAATTGCCAACGCTACGGAACCATAATCCGCCAACCTCCCGAGCTGCTCACCCCCATCTCGGCCCCATGGCCATTCGCGCAGTGGGGGGTAGATATCATTGGTCCTTTCCCTTTGGGCAAGGGCCAGACCAAGTTCGCTGTGGTTGCTGTGGATTACTTCACCAAGTGGGCCGAGGCCGAGGCGCTCTCCCACATAACGGAATCCAGGGTCACGTCCTTCGTATGGACGAATATCATATGTCGCTTTGGTATACCGCAGGCCATAGTGACAGACAATGGGAAGCAGTTTGACAACGCCAAGTTCAAAGACTTTTGCAGCAAACTTGGCATAAGTCATCTCAGCTCGTCCCCCGCACACCCGCAAGCAAATGGGCAGGTGGAGGCAGTCAACAAGATCATCAAGCGAGGCATCAAACTTAGACTGGACTCCAAGAAAGGCAGGTGGGCCGAGGAGCTACTAGAGGTTCTGTGGTCGTACCGGACCACCCAACGAGAGTCGACGGGTGAGACCCCGTTCTCCCTGGCCTTCGGCTCCGAAGCTGTAGTCCCGGTTGAGATCGGCATGCCATCTGACAGAGTAGAGCATTACGACCCTACGACGAATGAGGATGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGGAGAGCAATGGCCCAGCTACGCCTGGCGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAGCGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCGTTTGAGATTAAGGGCATAATCCGACCTGGGACGTACATATTGGCCGATCTGAAAGGTGACGTCCTCGCGCACCCGTGGAACGCGGAGCACCTGAAGCGTTATTATCCTTGA

Protein sequence

MPGDKLQLYLAVSDSAVSSALIRQEEARQNPVYYTSKAMTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAIELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLLGPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILDNPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGFSLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNKIIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSDRVEHYDPTTNEDELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRRVQAHVGALDPAWEGPFEIKGIIRPGTYILADLKGDVLAHPWNAEHLKRYYP

Homology

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BLAST of MC03g_new0106 vs. ExPASy Swiss-Prot
Match: P23074 (Pro-Pol polyprotein OS=Simian foamy virus type 1 OX=338478 GN=pol PE=1 SV=3)

HSP 1 Score: 115.5 bits (288), Expect = 2.4e-24
Identity = 91/320 (28.44%), Postives = 139/320 (43.44%), Query Frame = 0


Query: 366  HSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQPPELLTPISAPWPFAQ 425
            H+G  +   KV  + Y+WP L +D  K +R C  C          P +L P+    PF +
Sbjct: 816  HTGRDATFLKVSSK-YWWPNLRKDVVKSIRQCKQCLVTNATNLTSPPILRPVKPLKPFDK 875

Query: 426  WGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVTSFVWTNIICRFGIPQA 485
            + +D IGP P   G     VV VD  T +         + S     +  N++    IP+ 
Sbjct: 876  FYIDYIGPLPPSNGYLHVLVV-VDSMTGFVWLYPTKAPSTSATVKAL--NMLTSIAIPKV 935

Query: 486  IVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNKIIKRGIKLRLDSKKGR 545
            + +D G  F ++ F D+  + GI    S+P HPQ++G+VE  N  IKR +   L  +  +
Sbjct: 936  LHSDQGAAFTSSTFADWAKEKGIQLEFSTPYHPQSSGKVERKNSDIKRLLTKLLIGRPAK 995

Query: 546  WAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSDRVEHYDPTTNEDELLL 605
            W + L  V  +   +   S+  TP  L FG               V+   P  N D L L
Sbjct: 996  WYDLLPVVQLALNNSYSPSSKYTPHQLLFG---------------VDSNTPFANSDTLDL 1055

Query: 606  NLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRRVQAHVGALDPAWEGP 665
            + +  EE   + ++R + +Q        A  R  +  VG LV  RV A   +L P W  P
Sbjct: 1056 SRE--EELSLLQEIRSSLHQPTSP---PASSRSWSPSVGQLVQERV-ARPASLRPRWHKP 1110

Query: 666  FEIKGIIRPGTYILADLKGD 686
              I  ++ P T I+ D  G+
Sbjct: 1116 TAILEVVNPRTVIILDHLGN 1110

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BLAST of MC03g_new0106 vs. ExPASy Swiss-Prot
Match: P27401 (Pro-Pol polyprotein OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=pol PE=3 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 2.4e-24
Identity = 90/320 (28.12%), Postives = 137/320 (42.81%), Query Frame = 0


Query: 366  HSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQPPELLTPISAPWPFAQ 425
            H+G  S   KV  + Y+WP L +D  K +R C  C          P +L P     PF +
Sbjct: 816  HTGRDSTFLKVSSK-YWWPNLRKDVVKVIRQCKQCLVTNAATLAAPPILRPERPVKPFDK 875

Query: 426  WGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVTSFVWTNIICRFGIPQA 485
            + +D IGP P   G     VV VD  T +         + S     +  N++    +P+ 
Sbjct: 876  FFIDYIGPLPPSNGYLHVLVV-VDSMTGFVWLYPTKAPSTSATVKAL--NMLTSIAVPKV 935

Query: 486  IVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNKIIKRGIKLRLDSKKGR 545
            I +D G  F +A F D+    GI    S+P HPQ++G+VE  N  IKR +   L  +  +
Sbjct: 936  IHSDQGAAFTSATFADWAKNKGIQLEFSTPYHPQSSGKVERKNSDIKRLLTKLLVGRPAK 995

Query: 546  WAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSDRVEHYDPTTNEDELLL 605
            W + L  V  +   +   S+  TP  L FG               ++   P  N D L L
Sbjct: 996  WYDLLPVVQLALNNSYSPSSKYTPHQLLFG---------------IDSNTPFANSDTLDL 1055

Query: 606  NLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLRRVQAHVGALDPAWEGP 665
            + +  EE   + ++R + Y   +     A +R  +  VG LV  RV A   +L P W  P
Sbjct: 1056 SRE--EELSLLQEIRSSLY---LPSTPPASIRAWSPSVGQLVQERV-ARPASLRPRWHKP 1110

Query: 666  FEIKGIIRPGTYILADLKGD 686
              +  +I P   ++ D  G+
Sbjct: 1116 TPVLEVINPRAVVILDHLGN 1110

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BLAST of MC03g_new0106 vs. ExPASy Swiss-Prot
Match: P03359 (Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 6.6e-19
Identity = 96/355 (27.04%), Postives = 149/355 (41.97%), Query Frame = 0


Query: 346  LTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGT 405
            LTP+ G   ++ +H+     H G   L   V R     P L    ++    C  C     
Sbjct: 1326 LTPDRGKEFIQRLHQ---LTHLGPEKLLQLVNRTSLLIPNLQSAVREVTSQCQACAMTNA 1385

Query: 406  IIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITE 465
            +     E         P   W VD     P G+   ++ +V +D F+ W EA      T 
Sbjct: 1386 VTTY-RETGKRQRGDRPGVYWEVDFTEVKP-GRYGNRYLLVFIDTFSGWVEAFPTKTETA 1445

Query: 466  SRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVE 525
              V   +   I+ RFGIP+ + +DNG  F     +   ++LGI+        PQ++GQVE
Sbjct: 1446 LTVCKKILEEILPRFGIPKVLGSDNGPAFVAQVSQGLATQLGINWKLHCAYRPQSSGQVE 1505

Query: 526  AVNKIIKRGI-KLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEI 585
             +N+ IK  + KL L++    W   L   L   R T     G TP+ + +G    + +E 
Sbjct: 1506 RMNRTIKETLTKLALETGXKDWVALLPLALLRARNTPGR-FGLTPYEILYGGPPPI-LES 1565

Query: 586  GMPSDRVEHYDPTTNEDELLLNLDLLEERRAMA--QLRLAEYQGRMARHYNARVRPRAFQ 645
            G      +++ P      L  +L  LE  R     Q++     G +A        P  FQ
Sbjct: 1566 GGTLGPDDNFLPV-----LFTHLKALEVVRTQIWDQIKEVYKPGTVA-------IPHPFQ 1625

Query: 646  VGHLVLRRVQAHVGALDPAWEGPFEIKGIIRPGTYILADLKGDVLAHPWNAEHLK 698
            VG  VL R +   G+L+P W+GP+ +  ++   T +    K D +A   +A HLK
Sbjct: 1626 VGDQVLVR-RHRPGSLEPRWKGPYLV--LLTTPTAV----KVDGIAAWVHASHLK 1654

[Close]

BLAST of MC03g_new0106 vs. ExPASy Swiss-Prot
Match: Q9TTC1 (Gag-Pol polyprotein OS=Koala retrovirus OX=394239 GN=pro-pol PE=3 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 8.7e-19
Identity = 97/357 (27.17%), Postives = 150/357 (42.02%), Query Frame = 0


Query: 346  LTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNC--QRY 405
            LTP++G   ++ +H+     H G   L   V R  ++ P L    ++    C  C     
Sbjct: 1326 LTPDQGREFIQRLHQ---LTHLGPDKLLQLVGRTSFHIPNLQSVVREITSKCQVCAVTNA 1385

Query: 406  GTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHI 465
             T  R+P           P   W VD     P G+   ++ +V +D F+ W EA      
Sbjct: 1386 VTTYREPGRRQ---RGDRPGVYWEVDFTEVKP-GRYGNRYLLVFIDTFSGWVEAFPTKTE 1445

Query: 466  TESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQ 525
            T   V   +   I+ RFGIP+ + +DNG  F     +   ++LGI         PQ++GQ
Sbjct: 1446 TALTVCKKILEEILPRFGIPKVLGSDNGPAFVAQVSQGLATQLGIDWKLHCAYRPQSSGQ 1505

Query: 526  VEAVNKIIKRGI-KLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPV 585
            VE +N+ IK  + KL L++    W   L   L   R T  +  G TP+ +  G    V  
Sbjct: 1506 VERMNRTIKETLTKLALETGGKDWVTLLPLALLRARNTPGQ-FGLTPYEILHGGPPPVLA 1565

Query: 586  EIGMPSDRVEHYDPTTNEDELLLNLDLLEERRAMA--QLRLAEYQGRMARHYNARVRPRA 645
               +     + +        L  +L  LE  R     Q++ A   G +A        P  
Sbjct: 1566 SGEVVGSNGDFF------PVLFTHLKALEVVRTQIWDQIKEAYRPGTVA-------IPHP 1625

Query: 646  FQVGHLVLRRVQAHVGALDPAWEGPFEIKGIIRPGTYILADLKGDVLAHPWNAEHLK 698
            FQVG  VL R +   G+L+P W+GP+ +  ++   T +    K D +A   +A HLK
Sbjct: 1626 FQVGDRVLVR-RHRSGSLEPRWKGPYLV--LLTTPTAV----KVDGIAAWVHASHLK 1654

[Close]

BLAST of MC03g_new0106 vs. ExPASy Swiss-Prot
Match: P03360 (Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=pol PE=1 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 1.9e-18
Identity = 94/333 (28.23%), Postives = 146/333 (43.84%), Query Frame = 0


Query: 340  LPLLKCLTPEE-GLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 399
            LP  + L P   G  VL + H      H G   L+ +++R+ Y    + + A+     C 
Sbjct: 787  LPDGRLLLPRAVGRKVLEQTHR---ATHLGESKLT-ELVRKHYLICGIYRAARDITTRCV 846

Query: 400  NCQRYGTIIRQPPE-LLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEA 459
             C +         + L + I    P   W VD        KG  K+ +V VD F+ W EA
Sbjct: 847  ACAQVNPGAAPVEKGLNSRIRGAAPGEHWEVDFT-EMITAKGGYKYLLVLVDTFSGWVEA 906

Query: 460  EALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAH 519
                  T   V   +  +II RFG+P  I +DNG  F     +  C  L +S        
Sbjct: 907  YPAKRETSQVVIKHLIHDIIPRFGLPVQIGSDNGPAFVAKVTQQLCEALNVSWKLHCAYR 966

Query: 520  PQANGQVEAVNKIIKRGI-KLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGS 579
            PQ++GQVE +N+ +K  I KLR+++  G W   L + L   R T     G +PF + +G 
Sbjct: 967  PQSSGQVERMNRTLKETIAKLRIET-GGDWVSLLPQALLRARCTPGRE-GLSPFEILYGL 1026

Query: 580  EAVVPVEIGMPSDRVEHYDPTTNEDELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARV 639
            +  V   +G      +     TN+  LL +L  L+  R++A+  L +   ++ +    + 
Sbjct: 1027 KPPVVPRVG-----CDKLASITNQ-TLLKSLQALQATRSLARATLRD---QLPQKEAQQD 1086

Query: 640  RPRAFQVGHLVLRRVQAH-VGALDPAWEGPFEI 669
            R   FQ G LV   V+ H    L P W+GP+ +
Sbjct: 1087 RTPLFQPGDLVF--VKKHDFQQLGPRWDGPYTV 1101

[Close]

BLAST of MC03g_new0106 vs. NCBI nr
Match: XP_022156575.1 (uncharacterized protein LOC111023451 [Momordica charantia])

HSP 1 Score: 1112 bits (2875), Expect = 0.0
Identity = 556/570 (97.54%), Postives = 559/570 (98.07%), Query Frame = 0


Query: 39  MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAI 98
           MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMK AI
Sbjct: 1   MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKXAI 60

Query: 99  ELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 158
           ELSEYDIQF PRTALKGQ  A+FIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL
Sbjct: 61  ELSEYDIQFXPRTALKGQXXABFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 120

Query: 159 GPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQ 218
           GPGGERFEYALRFSFRTSNNEAEYEAFI GLRIARTLGASC+KVFSDSQLVVSQIKDEYQ
Sbjct: 121 GPGGERFEYALRFSFRTSNNEAEYEAFIXGLRIARTLGASCVKVFSDSQLVVSQIKDEYQ 180

Query: 219 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 278
           AKDTRME YLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD
Sbjct: 181 AKDTRMEXYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 240

Query: 279 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 338
           NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF
Sbjct: 241 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 300

Query: 339 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 398
           SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAK+FVRTCD
Sbjct: 301 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKQFVRTCD 360

Query: 399 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAE 458
           NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIG FPLGKGQTKFAVVAVDYFTKW EAE
Sbjct: 361 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGHFPLGKGQTKFAVVAVDYFTKWVEAE 420

Query: 459 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP 518
           ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP
Sbjct: 421 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP 480

Query: 519 QANGQVEAVNKIIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEA 578
           QANGQVEAVNKIIKRGIKLRLDSKKGRW EEL EVLWSY TTQRESTGETPFSLAFGSEA
Sbjct: 481 QANGQVEAVNKIIKRGIKLRLDSKKGRWVEELPEVLWSYWTTQRESTGETPFSLAFGSEA 540

Query: 579 VVPVEIGMPSDRVEHYDPTTNEDELLLNLD 608
           VVPVEIGMPSDRVEHYDPTTNEDELLLNLD
Sbjct: 541 VVPVEIGMPSDRVEHYDPTTNEDELLLNLD 570

[Close]

BLAST of MC03g_new0106 vs. NCBI nr
Match: XP_022157799.1 (uncharacterized protein LOC111024419 [Momordica charantia])

HSP 1 Score: 1070 bits (2768), Expect = 0.0
Identity = 534/624 (85.58%), Postives = 567/624 (90.87%), Query Frame = 0


Query: 1   MPGDKLQLYLAVSDSAVSSALIRQEEARQNPVYYTSKAMTEAETRYPQMEKLALALVTSA 60
           +PGDKL LYLAVSDSAVSSAL++QEEA+Q+PVYYTSKAMTEAETRYPQMEKLALALVT A
Sbjct: 86  LPGDKLHLYLAVSDSAVSSALVKQEEAQQSPVYYTSKAMTEAETRYPQMEKLALALVTLA 145

Query: 61  RRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAIELSEYDIQFEPRTALKGQAAAD 120
           RRLRPYFQAHTVVVLTNLPLK++FHKPE SGRLMKWA+ELSEYD+QFEPR+ LKGQA AD
Sbjct: 146 RRLRPYFQAHTVVVLTNLPLKNVFHKPETSGRLMKWAMELSEYDVQFEPRSTLKGQAVAD 205

Query: 121 FIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLLGPGGERFEYALRFSFRTSNNEA 180
           FIAELTPP E SESD  WT+YVDGSS+EKGCGA VLLL  GGE FEYALRFSF TSNNEA
Sbjct: 206 FIAELTPPPEPSESDPSWTIYVDGSSSEKGCGARVLLLALGGEWFEYALRFSFWTSNNEA 265

Query: 181 EYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSYLAQFRT 240
           EYEA IAGLRIAR LGASCIK FSDSQLVVSQIK+EY+AKD+RMEKYL KVRSYL QFRT
Sbjct: 266 EYEALIAGLRIARALGASCIKXFSDSQLVVSQIKEEYEAKDSRMEKYLSKVRSYLTQFRT 325

Query: 241 YEVSRIPRAENSNADALAKLASAYETDLARSVPVEILDNPSILEPDLMEVGAPESSWMDP 300
            EVSR+PRAENSNADALAKLASAYETDL RSVPVEILDNPSILEPD ME+ A E SWMDP
Sbjct: 326 SEVSRVPRAENSNADALAKLASAYETDLVRSVPVEILDNPSILEPDQMEIDALEPSWMDP 385

Query: 301 ITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGFSLPLLKCLTPEEGLYVLREIHE 360
           I DFIRGNSPQDPKE +K+AR+ A  ++RDGALYRR FSLPLLKCLT EEGLYVL+EIHE
Sbjct: 386 IADFIRGNSPQDPKEHKKMARKAAPFIIRDGALYRRNFSLPLLKCLTLEEGLYVLKEIHE 445

Query: 361 GVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQPPELLTPISAP 420
           G+CGNHSGARSLSAKV+RQGYYWPTL QDAK+FVRT DNCQR+G II QPPELLTPISA 
Sbjct: 446 GLCGNHSGARSLSAKVVRQGYYWPTLDQDAKEFVRTFDNCQRFGNIIHQPPELLTPISAL 505

Query: 421 WPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVTSFVWTNIICRF 480
           WPF QWG+DIIGPF LGKG TKFAVVAVDYFTKWAEA  LSHITESRVTSFVW NI+CRF
Sbjct: 506 WPFVQWGIDIIGPFLLGKGHTKFAVVAVDYFTKWAEAGVLSHITESRVTSFVWVNIVCRF 565

Query: 481 GIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNKIIKRGIKLRLD 540
           GIP AIVTDN KQFDN  FKDFC KLGISHLSSSPAH QANGQVEAVNKIIKRGIKLRLD
Sbjct: 566 GIPHAIVTDNEKQFDNVNFKDFCRKLGISHLSSSPAHLQANGQVEAVNKIIKRGIKLRLD 625

Query: 541 SKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSDRVEHYDPTTNE 600
           S+KGRWA EL EVLWSYRTTQRESTGETPFSLAF SEAVVPVEIGM SDRV HY+PT NE
Sbjct: 626 SRKGRWANELPEVLWSYRTTQRESTGETPFSLAFVSEAVVPVEIGMTSDRVAHYEPTVNE 685

Query: 601 DELLLNLDLLEERRAMAQLRLAEY 624
           +ELLLNLDLLEERR +AQLRL EY
Sbjct: 686 EELLLNLDLLEERREVAQLRLTEY 709

[Close]

BLAST of MC03g_new0106 vs. NCBI nr
Match: XP_022158579.1 (uncharacterized protein LOC111025033 [Momordica charantia])

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 444/716 (62.01%), Postives = 549/716 (76.68%), Query Frame = 0


Query: 3   GDKLQLYLAVSDSAVSSALIRQEEARQNPVYYTSKAMTEAETRYPQMEKLALALVTSARR 62
           GD L LYLAVS++AVSSALI++E+ RQ+P+YYTSKAM +AE RYP MEKLALALVTSARR
Sbjct: 172 GDXLYLYLAVSNTAVSSALIKEEDGRQSPIYYTSKAMVDAELRYPPMEKLALALVTSARR 231

Query: 63  LRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAIELSEYDIQFEPRTALKGQAAADFI 122
           LRPYFQAH ++VL N PL+ I  KPE SGRL+KWA+EL+EYDI ++PRTA+KGQ   DFI
Sbjct: 232 LRPYFQAHKIMVLANFPLRQILQKPETSGRLVKWAVELNEYDIHYKPRTAMKGQVVVDFI 291

Query: 123 AELTP---------PSELSESDLPWTVYVDGSSNEKGCGAGVLLLGPGGERFEYALRFSF 182
           AELTP         P  + E  L WT+YVDGSSN +GCGAG+LL+ P  ERFEYAL+F+F
Sbjct: 292 AELTPIEPQVYSIDPQVVEEEQL-WTLYVDGSSNFRGCGAGLLLISPDKERFEYALQFNF 351

Query: 183 RTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRS 242
             SNNE EYEA +A L++ R +G   I + SDSQL+V Q+K+++QA++ RM KYL +VR+
Sbjct: 352 HASNNEVEYEALLAALKVYRYVGVRNILILSDSQLIVGQVKEKFQARELRMAKYLSQVRN 411

Query: 243 YLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILDNPSILEPDLMEV--- 302
            L QF  YE+ +IP A+N NAD LA+LA AYETDL R+VPVEIL  PSI   + M++   
Sbjct: 412 QLDQFSKYEIRQIPHAQNVNADVLARLAVAYETDLGRTVPVEILPGPSIEAQEAMDIDNQ 471

Query: 303 GAPESSWMDPITDFIR-GNSPQDPKERRKLARRTARLVVRDGALYRRGFSLPLLKCLTPE 362
           G PE +W  P+  +++ G  P +  E ++L RR AR V++D  LY+ G+SLPLLKCLTP+
Sbjct: 472 GQPEENWKSPLIKYLKDGILPTEKIEAQQLQRRAARYVLKDDNLYKIGYSLPLLKCLTPD 531

Query: 363 EGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQ 422
           E  YV+REIHEGVCG+HSGARSLS K+IRQGYYWPT+  D K F + CD CQR+ T+ RQ
Sbjct: 532 EADYVMREIHEGVCGSHSGARSLSHKIIRQGYYWPTMLNDTKXFTKKCDKCQRFATVPRQ 591

Query: 423 PPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVT 482
           PPE LT I +PWPFAQWG+D+IGP PLGKGQTKFAVVAVDYFTKWAEA+AL+ ITE +VT
Sbjct: 592 PPEPLTSIMSPWPFAQWGIDLIGPLPLGKGQTKFAVVAVDYFTKWAEAKALATITEKKVT 651

Query: 483 SFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNK 542
            F+W NIICRFGIP AI++DNGKQFDNA F++F  +LGI H+ SSPAHPQANGQVEAVNK
Sbjct: 652 YFIWKNIICRFGIPYAIISDNGKQFDNAAFREFFRELGIKHICSSPAHPQANGQVEAVNK 711

Query: 543 IIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSD 602
           +IKR +K RL+  KG WAEEL   LW+Y TT   STGETPFSL+FGSEAVVPVEIG+PS 
Sbjct: 712 VIKRTLKTRLEKLKGLWAEELPNALWAYXTTPCTSTGETPFSLSFGSEAVVPVEIGLPSP 771

Query: 603 RVEHYDPTTNEDELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLR 662
           RVE +    N + + LNLDLLEE R  A+LR AEY+ R+AR+YN++V+ R F+ G LVLR
Sbjct: 772 RVEQFTLLDNNESVKLNLDLLEEHRETARLRTAEYKNRIARYYNSKVKKRDFKEGDLVLR 831

Query: 663 RVQAHV-----GALDPAWEGPFEIKGIIRPGTYILADLKGDVLAHPWNAEHLKRYY 700
           +V  +      GAL P WEGP++   ++R GTY L D +G  L HPW+ +HLKRYY
Sbjct: 832 KVMPNTKDSTSGALGPTWEGPYKXTKVVRIGTYALEDQRGKALLHPWHXQHLKRYY 886

[Close]

BLAST of MC03g_new0106 vs. NCBI nr
Match: XP_022149417.1 (protein NYNRIN-like [Momordica charantia])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 432/554 (77.98%), Postives = 483/554 (87.18%), Query Frame = 0


Query: 39  MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAI 98
           MTEAETRYPQMEKLALALVTSAR+LRPYFQAHT++VLTNLPLK+IF KPE SGRL+KWA+
Sbjct: 1   MTEAETRYPQMEKLALALVTSARKLRPYFQAHTILVLTNLPLKNIFLKPETSGRLVKWAL 60

Query: 99  ELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 158
           ELSEYDIQF PRT LK QA ADFI ELTPPS+ +ESDL WT+YVDGSSNE+GCGAG+LLL
Sbjct: 61  ELSEYDIQFGPRTVLKEQAVADFIVELTPPSQSTESDLSWTIYVDGSSNERGCGAGILLL 120

Query: 159 GPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQ 218
             GGERFEYALRF+FRTSNNEAEYEA +AGLR+A+ LGA+ +KVFSDSQL+V+QIK+EYQ
Sbjct: 121 AQGGERFEYALRFNFRTSNNEAEYEALLAGLRVAKRLGANHVKVFSDSQLIVNQIKEEYQ 180

Query: 219 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 278
             D RMEKYL                              KLASAYETDLARSVPVEILD
Sbjct: 181 TNDPRMEKYL-----------------------------TKLASAYETDLARSVPVEILD 240

Query: 279 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 338
           +PSILEPD+MEV  P  SW+DPI +FI+GN PQDPKE++K+ARR AR  +R+GALYRRGF
Sbjct: 241 SPSILEPDVMEVDTPSPSWVDPIVEFIKGNPPQDPKEQKKMARRAARFTLREGALYRRGF 300

Query: 339 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 398
           SLPLLKC+TPEEGLY+LREI+EG CGN SGARSLSAKV+RQGYYWPT+ QDA++FV+TCD
Sbjct: 301 SLPLLKCVTPEEGLYILREIYEGACGNDSGARSLSAKVVRQGYYWPTIEQDARQFVKTCD 360

Query: 399 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAE 458
           NCQR+  II QP ELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVA+DYFTKWAEAE
Sbjct: 361 NCQRFANIIHQPLELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAMDYFTKWAEAE 420

Query: 459 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP 518
           ALSHITESRVTSF+W N++CRFGIP AIVTDNGKQFDNAKFKDFCS LGI HLSSSPAHP
Sbjct: 421 ALSHITESRVTSFIWANVVCRFGIPNAIVTDNGKQFDNAKFKDFCSNLGIRHLSSSPAHP 480

Query: 519 QANGQVEAVNKIIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEA 578
           +ANGQVEAVNKIIKR +KLRLDS+ GRWAEEL EVLWSY+TT RESTGETPFSLAFGSEA
Sbjct: 481 KANGQVEAVNKIIKRELKLRLDSRMGRWAEELPEVLWSYQTTPRESTGETPFSLAFGSEA 525

Query: 579 VVPVEIGMPSDRVE 592
           +VPVEIG+P++RVE
Sbjct: 541 IVPVEIGIPTNRVE 525

[Close]

BLAST of MC03g_new0106 vs. NCBI nr
Match: XP_022143486.1 (uncharacterized protein LOC111013362 [Momordica charantia])

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 441/460 (95.87%), Postives = 445/460 (96.74%), Query Frame = 0


Query: 39  MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAI 98
           MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHK EASGRLMKWAI
Sbjct: 1   MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKSEASGRLMKWAI 60

Query: 99  ELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 158
           ELSEYDIQFEPRTALKGQAAADF+ ELTP SEL ESDLPWTVYVDGSSNEKGCG GVLLL
Sbjct: 61  ELSEYDIQFEPRTALKGQAAADFJXELTPXSELRESDLPWTVYVDGSSNEKGCGXGVLLL 120

Query: 159 GPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQ 218
           GPGGERFE ALRF FRTSNNEAEYEAFIAGLRIAR LGASC+KVFSDSQLVVSQIKDEYQ
Sbjct: 121 GPGGERFEXALRFGFRTSNNEAEYEAFIAGLRIARALGASCVKVFSDSQLVVSQIKDEYQ 180

Query: 219 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 278
           AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD
Sbjct: 181 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 240

Query: 279 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 338
           NPSI EPDLME+GAPESSWMDPI DFIRGNSPQDPKERRKLARR AR VVRDGALYRRGF
Sbjct: 241 NPSISEPDLMEIGAPESSWMDPIADFIRGNSPQDPKERRKLARRAARFVVRDGALYRRGF 300

Query: 339 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 398
           SLPLL+CLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD
Sbjct: 301 SLPLLRCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 360

Query: 399 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAE 458
           NCQRYGTII QPPELLTPISAPWPFAQWGVDIIGPFPL KGQTKFAVVAVDYFTKWAE E
Sbjct: 361 NCQRYGTIIHQPPELLTPISAPWPFAQWGVDIIGPFPLSKGQTKFAVVAVDYFTKWAEVE 420

Query: 459 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAK 498
           ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAK
Sbjct: 421 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAK 460

[Close]

BLAST of MC03g_new0106 vs. ExPASy TrEMBL
Match: A0A6J1DQP4 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023451 PE=4 SV=1)

HSP 1 Score: 1112 bits (2877), Expect = 0.0
Identity = 557/570 (97.72%), Postives = 559/570 (98.07%), Query Frame = 0


Query: 39  MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAI 98
           MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMK AI
Sbjct: 1   MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKXAI 60

Query: 99  ELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 158
           ELSEYDIQF PRTALKGQ  ADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL
Sbjct: 61  ELSEYDIQFXPRTALKGQXXADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 120

Query: 159 GPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQ 218
           GPGGERFEYALRFSFRTSNNEAEYEAFI GLRIARTLGASC+KVFSDSQLVVSQIKDEYQ
Sbjct: 121 GPGGERFEYALRFSFRTSNNEAEYEAFIXGLRIARTLGASCVKVFSDSQLVVSQIKDEYQ 180

Query: 219 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 278
           AKDTRME YLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD
Sbjct: 181 AKDTRMEXYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 240

Query: 279 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 338
           NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF
Sbjct: 241 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 300

Query: 339 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 398
           SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAK+FVRTCD
Sbjct: 301 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKQFVRTCD 360

Query: 399 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAE 458
           NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIG FPLGKGQTKFAVVAVDYFTKW EAE
Sbjct: 361 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGHFPLGKGQTKFAVVAVDYFTKWVEAE 420

Query: 459 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP 518
           ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP
Sbjct: 421 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP 480

Query: 519 QANGQVEAVNKIIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEA 578
           QANGQVEAVNKIIKRGIKLRLDSKKGRW EEL EVLWSY TTQRESTGETPFSLAFGSEA
Sbjct: 481 QANGQVEAVNKIIKRGIKLRLDSKKGRWVEELPEVLWSYWTTQRESTGETPFSLAFGSEA 540

Query: 579 VVPVEIGMPSDRVEHYDPTTNEDELLLNLD 608
           VVPVEIGMPSDRVEHYDPTTNEDELLLNLD
Sbjct: 541 VVPVEIGMPSDRVEHYDPTTNEDELLLNLD 570

[Close]

BLAST of MC03g_new0106 vs. ExPASy TrEMBL
Match: A0A6J1DXK8 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024419 PE=4 SV=1)

HSP 1 Score: 1070 bits (2768), Expect = 0.0
Identity = 534/624 (85.58%), Postives = 567/624 (90.87%), Query Frame = 0


Query: 1   MPGDKLQLYLAVSDSAVSSALIRQEEARQNPVYYTSKAMTEAETRYPQMEKLALALVTSA 60
           +PGDKL LYLAVSDSAVSSAL++QEEA+Q+PVYYTSKAMTEAETRYPQMEKLALALVT A
Sbjct: 86  LPGDKLHLYLAVSDSAVSSALVKQEEAQQSPVYYTSKAMTEAETRYPQMEKLALALVTLA 145

Query: 61  RRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAIELSEYDIQFEPRTALKGQAAAD 120
           RRLRPYFQAHTVVVLTNLPLK++FHKPE SGRLMKWA+ELSEYD+QFEPR+ LKGQA AD
Sbjct: 146 RRLRPYFQAHTVVVLTNLPLKNVFHKPETSGRLMKWAMELSEYDVQFEPRSTLKGQAVAD 205

Query: 121 FIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLLGPGGERFEYALRFSFRTSNNEA 180
           FIAELTPP E SESD  WT+YVDGSS+EKGCGA VLLL  GGE FEYALRFSF TSNNEA
Sbjct: 206 FIAELTPPPEPSESDPSWTIYVDGSSSEKGCGARVLLLALGGEWFEYALRFSFWTSNNEA 265

Query: 181 EYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSYLAQFRT 240
           EYEA IAGLRIAR LGASCIK FSDSQLVVSQIK+EY+AKD+RMEKYL KVRSYL QFRT
Sbjct: 266 EYEALIAGLRIARALGASCIKXFSDSQLVVSQIKEEYEAKDSRMEKYLSKVRSYLTQFRT 325

Query: 241 YEVSRIPRAENSNADALAKLASAYETDLARSVPVEILDNPSILEPDLMEVGAPESSWMDP 300
            EVSR+PRAENSNADALAKLASAYETDL RSVPVEILDNPSILEPD ME+ A E SWMDP
Sbjct: 326 SEVSRVPRAENSNADALAKLASAYETDLVRSVPVEILDNPSILEPDQMEIDALEPSWMDP 385

Query: 301 ITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGFSLPLLKCLTPEEGLYVLREIHE 360
           I DFIRGNSPQDPKE +K+AR+ A  ++RDGALYRR FSLPLLKCLT EEGLYVL+EIHE
Sbjct: 386 IADFIRGNSPQDPKEHKKMARKAAPFIIRDGALYRRNFSLPLLKCLTLEEGLYVLKEIHE 445

Query: 361 GVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQPPELLTPISAP 420
           G+CGNHSGARSLSAKV+RQGYYWPTL QDAK+FVRT DNCQR+G II QPPELLTPISA 
Sbjct: 446 GLCGNHSGARSLSAKVVRQGYYWPTLDQDAKEFVRTFDNCQRFGNIIHQPPELLTPISAL 505

Query: 421 WPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVTSFVWTNIICRF 480
           WPF QWG+DIIGPF LGKG TKFAVVAVDYFTKWAEA  LSHITESRVTSFVW NI+CRF
Sbjct: 506 WPFVQWGIDIIGPFLLGKGHTKFAVVAVDYFTKWAEAGVLSHITESRVTSFVWVNIVCRF 565

Query: 481 GIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNKIIKRGIKLRLD 540
           GIP AIVTDN KQFDN  FKDFC KLGISHLSSSPAH QANGQVEAVNKIIKRGIKLRLD
Sbjct: 566 GIPHAIVTDNEKQFDNVNFKDFCRKLGISHLSSSPAHLQANGQVEAVNKIIKRGIKLRLD 625

Query: 541 SKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSDRVEHYDPTTNE 600
           S+KGRWA EL EVLWSYRTTQRESTGETPFSLAF SEAVVPVEIGM SDRV HY+PT NE
Sbjct: 626 SRKGRWANELPEVLWSYRTTQRESTGETPFSLAFVSEAVVPVEIGMTSDRVAHYEPTVNE 685

Query: 601 DELLLNLDLLEERRAMAQLRLAEY 624
           +ELLLNLDLLEERR +AQLRL EY
Sbjct: 686 EELLLNLDLLEERREVAQLRLTEY 709

[Close]

BLAST of MC03g_new0106 vs. ExPASy TrEMBL
Match: A0A6J1DZU0 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025033 PE=4 SV=1)

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 444/716 (62.01%), Postives = 549/716 (76.68%), Query Frame = 0


Query: 3   GDKLQLYLAVSDSAVSSALIRQEEARQNPVYYTSKAMTEAETRYPQMEKLALALVTSARR 62
           GD L LYLAVS++AVSSALI++E+ RQ+P+YYTSKAM +AE RYP MEKLALALVTSARR
Sbjct: 172 GDXLYLYLAVSNTAVSSALIKEEDGRQSPIYYTSKAMVDAELRYPPMEKLALALVTSARR 231

Query: 63  LRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAIELSEYDIQFEPRTALKGQAAADFI 122
           LRPYFQAH ++VL N PL+ I  KPE SGRL+KWA+EL+EYDI ++PRTA+KGQ   DFI
Sbjct: 232 LRPYFQAHKIMVLANFPLRQILQKPETSGRLVKWAVELNEYDIHYKPRTAMKGQVVVDFI 291

Query: 123 AELTP---------PSELSESDLPWTVYVDGSSNEKGCGAGVLLLGPGGERFEYALRFSF 182
           AELTP         P  + E  L WT+YVDGSSN +GCGAG+LL+ P  ERFEYAL+F+F
Sbjct: 292 AELTPIEPQVYSIDPQVVEEEQL-WTLYVDGSSNFRGCGAGLLLISPDKERFEYALQFNF 351

Query: 183 RTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRS 242
             SNNE EYEA +A L++ R +G   I + SDSQL+V Q+K+++QA++ RM KYL +VR+
Sbjct: 352 HASNNEVEYEALLAALKVYRYVGVRNILILSDSQLIVGQVKEKFQARELRMAKYLSQVRN 411

Query: 243 YLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILDNPSILEPDLMEV--- 302
            L QF  YE+ +IP A+N NAD LA+LA AYETDL R+VPVEIL  PSI   + M++   
Sbjct: 412 QLDQFSKYEIRQIPHAQNVNADVLARLAVAYETDLGRTVPVEILPGPSIEAQEAMDIDNQ 471

Query: 303 GAPESSWMDPITDFIR-GNSPQDPKERRKLARRTARLVVRDGALYRRGFSLPLLKCLTPE 362
           G PE +W  P+  +++ G  P +  E ++L RR AR V++D  LY+ G+SLPLLKCLTP+
Sbjct: 472 GQPEENWKSPLIKYLKDGILPTEKIEAQQLQRRAARYVLKDDNLYKIGYSLPLLKCLTPD 531

Query: 363 EGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCDNCQRYGTIIRQ 422
           E  YV+REIHEGVCG+HSGARSLS K+IRQGYYWPT+  D K F + CD CQR+ T+ RQ
Sbjct: 532 EADYVMREIHEGVCGSHSGARSLSHKIIRQGYYWPTMLNDTKXFTKKCDKCQRFATVPRQ 591

Query: 423 PPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAEALSHITESRVT 482
           PPE LT I +PWPFAQWG+D+IGP PLGKGQTKFAVVAVDYFTKWAEA+AL+ ITE +VT
Sbjct: 592 PPEPLTSIMSPWPFAQWGIDLIGPLPLGKGQTKFAVVAVDYFTKWAEAKALATITEKKVT 651

Query: 483 SFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHPQANGQVEAVNK 542
            F+W NIICRFGIP AI++DNGKQFDNA F++F  +LGI H+ SSPAHPQANGQVEAVNK
Sbjct: 652 YFIWKNIICRFGIPYAIISDNGKQFDNAAFREFFRELGIKHICSSPAHPQANGQVEAVNK 711

Query: 543 IIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEAVVPVEIGMPSD 602
           +IKR +K RL+  KG WAEEL   LW+Y TT   STGETPFSL+FGSEAVVPVEIG+PS 
Sbjct: 712 VIKRTLKTRLEKLKGLWAEELPNALWAYXTTPCTSTGETPFSLSFGSEAVVPVEIGLPSP 771

Query: 603 RVEHYDPTTNEDELLLNLDLLEERRAMAQLRLAEYQGRMARHYNARVRPRAFQVGHLVLR 662
           RVE +    N + + LNLDLLEE R  A+LR AEY+ R+AR+YN++V+ R F+ G LVLR
Sbjct: 772 RVEQFTLLDNNESVKLNLDLLEEHRETARLRTAEYKNRIARYYNSKVKKRDFKEGDLVLR 831

Query: 663 RVQAHV-----GALDPAWEGPFEIKGIIRPGTYILADLKGDVLAHPWNAEHLKRYY 700
           +V  +      GAL P WEGP++   ++R GTY L D +G  L HPW+ +HLKRYY
Sbjct: 832 KVMPNTKDSTSGALGPTWEGPYKXTKVVRIGTYALEDQRGKALLHPWHXQHLKRYY 886

[Close]

BLAST of MC03g_new0106 vs. ExPASy TrEMBL
Match: A0A6J1D7W6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111017848 PE=4 SV=1)

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 432/554 (77.98%), Postives = 483/554 (87.18%), Query Frame = 0


Query: 39  MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAI 98
           MTEAETRYPQMEKLALALVTSAR+LRPYFQAHT++VLTNLPLK+IF KPE SGRL+KWA+
Sbjct: 1   MTEAETRYPQMEKLALALVTSARKLRPYFQAHTILVLTNLPLKNIFLKPETSGRLVKWAL 60

Query: 99  ELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 158
           ELSEYDIQF PRT LK QA ADFI ELTPPS+ +ESDL WT+YVDGSSNE+GCGAG+LLL
Sbjct: 61  ELSEYDIQFGPRTVLKEQAVADFIVELTPPSQSTESDLSWTIYVDGSSNERGCGAGILLL 120

Query: 159 GPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQ 218
             GGERFEYALRF+FRTSNNEAEYEA +AGLR+A+ LGA+ +KVFSDSQL+V+QIK+EYQ
Sbjct: 121 AQGGERFEYALRFNFRTSNNEAEYEALLAGLRVAKRLGANHVKVFSDSQLIVNQIKEEYQ 180

Query: 219 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 278
             D RMEKYL                              KLASAYETDLARSVPVEILD
Sbjct: 181 TNDPRMEKYL-----------------------------TKLASAYETDLARSVPVEILD 240

Query: 279 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 338
           +PSILEPD+MEV  P  SW+DPI +FI+GN PQDPKE++K+ARR AR  +R+GALYRRGF
Sbjct: 241 SPSILEPDVMEVDTPSPSWVDPIVEFIKGNPPQDPKEQKKMARRAARFTLREGALYRRGF 300

Query: 339 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 398
           SLPLLKC+TPEEGLY+LREI+EG CGN SGARSLSAKV+RQGYYWPT+ QDA++FV+TCD
Sbjct: 301 SLPLLKCVTPEEGLYILREIYEGACGNDSGARSLSAKVVRQGYYWPTIEQDARQFVKTCD 360

Query: 399 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAE 458
           NCQR+  II QP ELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVA+DYFTKWAEAE
Sbjct: 361 NCQRFANIIHQPLELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAMDYFTKWAEAE 420

Query: 459 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAKFKDFCSKLGISHLSSSPAHP 518
           ALSHITESRVTSF+W N++CRFGIP AIVTDNGKQFDNAKFKDFCS LGI HLSSSPAHP
Sbjct: 421 ALSHITESRVTSFIWANVVCRFGIPNAIVTDNGKQFDNAKFKDFCSNLGIRHLSSSPAHP 480

Query: 519 QANGQVEAVNKIIKRGIKLRLDSKKGRWAEELLEVLWSYRTTQRESTGETPFSLAFGSEA 578
           +ANGQVEAVNKIIKR +KLRLDS+ GRWAEEL EVLWSY+TT RESTGETPFSLAFGSEA
Sbjct: 481 KANGQVEAVNKIIKRELKLRLDSRMGRWAEELPEVLWSYQTTPRESTGETPFSLAFGSEA 525

Query: 579 VVPVEIGMPSDRVE 592
           +VPVEIG+P++RVE
Sbjct: 541 IVPVEIGIPTNRVE 525

[Close]

BLAST of MC03g_new0106 vs. ExPASy TrEMBL
Match: A0A6J1CQD7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013362 PE=4 SV=1)

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 441/460 (95.87%), Postives = 445/460 (96.74%), Query Frame = 0


Query: 39  MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKPEASGRLMKWAI 98
           MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHK EASGRLMKWAI
Sbjct: 1   MTEAETRYPQMEKLALALVTSARRLRPYFQAHTVVVLTNLPLKSIFHKSEASGRLMKWAI 60

Query: 99  ELSEYDIQFEPRTALKGQAAADFIAELTPPSELSESDLPWTVYVDGSSNEKGCGAGVLLL 158
           ELSEYDIQFEPRTALKGQAAADF+ ELTP SEL ESDLPWTVYVDGSSNEKGCG GVLLL
Sbjct: 61  ELSEYDIQFEPRTALKGQAAADFLXELTPXSELRESDLPWTVYVDGSSNEKGCGXGVLLL 120

Query: 159 GPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQ 218
           GPGGERFE ALRF FRTSNNEAEYEAFIAGLRIAR LGASC+KVFSDSQLVVSQIKDEYQ
Sbjct: 121 GPGGERFEXALRFGFRTSNNEAEYEAFIAGLRIARALGASCVKVFSDSQLVVSQIKDEYQ 180

Query: 219 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 278
           AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD
Sbjct: 181 AKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADALAKLASAYETDLARSVPVEILD 240

Query: 279 NPSILEPDLMEVGAPESSWMDPITDFIRGNSPQDPKERRKLARRTARLVVRDGALYRRGF 338
           NPSI EPDLME+GAPESSWMDPI DFIRGNSPQDPKERRKLARR AR VVRDGALYRRGF
Sbjct: 241 NPSISEPDLMEIGAPESSWMDPIADFIRGNSPQDPKERRKLARRAARFVVRDGALYRRGF 300

Query: 339 SLPLLKCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 398
           SLPLL+CLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD
Sbjct: 301 SLPLLRCLTPEEGLYVLREIHEGVCGNHSGARSLSAKVIRQGYYWPTLSQDAKKFVRTCD 360

Query: 399 NCQRYGTIIRQPPELLTPISAPWPFAQWGVDIIGPFPLGKGQTKFAVVAVDYFTKWAEAE 458
           NCQRYGTII QPPELLTPISAPWPFAQWGVDIIGPFPL KGQTKFAVVAVDYFTKWAE E
Sbjct: 361 NCQRYGTIIHQPPELLTPISAPWPFAQWGVDIIGPFPLSKGQTKFAVVAVDYFTKWAEVE 420

Query: 459 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAK 498
           ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAK
Sbjct: 421 ALSHITESRVTSFVWTNIICRFGIPQAIVTDNGKQFDNAK 460

[Close]

BLAST of MC03g_new0106 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 8.3e-09
Identity = 38/125 (30.40%), Postives = 58/125 (46.40%), Query Frame = 0


Query: 139 TVYVDGSS--NEKGCGAGVLLLGPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLG 198
           T+  DG+S  N    GAG +L         Y        +NN AEY A + GLR A   G
Sbjct: 156 TIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKG 215

Query: 199 ASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADA 258
              + V  DS LV  Q++  ++    +M +   + +  +  F+T+++  I R +NS AD 
Sbjct: 216 FKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADK 275

Query: 259 LAKLA 262
            A  A
Sbjct: 276 QANSA 280

[Close]

BLAST of MC03g_new0106 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 8.3e-09
Identity = 38/125 (30.40%), Postives = 58/125 (46.40%), Query Frame = 0


Query: 139 TVYVDGSS--NEKGCGAGVLLLGPGGERFEYALRFSFRTSNNEAEYEAFIAGLRIARTLG 198
           T+  DG+S  N    GAG +L         Y        +NN AEY A + GLR A   G
Sbjct: 156 TIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKG 215

Query: 199 ASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSYLAQFRTYEVSRIPRAENSNADA 258
              + V  DS LV  Q++  ++    +M +   + +  +  F+T+++  I R +NS AD 
Sbjct: 216 FKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADK 275

Query: 259 LAKLA 262
            A  A
Sbjct: 276 QANSA 280

[Close]

BLAST of MC03g_new0106 vs. TAIR 10
Match: AT5G51080.1 (RNase H family protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-06
Identity = 45/148 (30.41%), Postives = 73/148 (49.32%), Query Frame = 0


Query: 122 IAELTPPSELSESDLPWTVYVDGSS--NEKGCGAGVLLLGPGGERFEYALRFSFR----- 181
           +AEL P ++ S       +  DG+S  N    GA  +L    G     +L F  R     
Sbjct: 172 LAELEPSADTSYETC--IIEFDGASKGNPGLSGAAAVLKTEDG-----SLIFKMRQGLGI 231

Query: 182 TSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSY 241
            +NN AEY   I GL+ A   G + IKV +DS+LV  Q+K +++     + K   + +  
Sbjct: 232 ATNNAAEYHGLILGLKHAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQL 291

Query: 242 LAQFRTYEVSRIPRAENSNADALAKLAS 263
             +  ++E+S + R+ NS+AD  A +A+
Sbjct: 292 SDKCLSFEISHVLRSLNSDADEQANMAA 312

[Close]

BLAST of MC03g_new0106 vs. TAIR 10
Match: AT5G51080.2 (RNase H family protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-06
Identity = 45/148 (30.41%), Postives = 73/148 (49.32%), Query Frame = 0


Query: 122 IAELTPPSELSESDLPWTVYVDGSS--NEKGCGAGVLLLGPGGERFEYALRFSFR----- 181
           +AEL P ++ S       +  DG+S  N    GA  +L    G     +L F  R     
Sbjct: 172 LAELEPSADTSYETC--IIEFDGASKGNPGLSGAAAVLKTEDG-----SLIFKMRQGLGI 231

Query: 182 TSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSY 241
            +NN AEY   I GL+ A   G + IKV +DS+LV  Q+K +++     + K   + +  
Sbjct: 232 ATNNAAEYHGLILGLKHAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQL 291

Query: 242 LAQFRTYEVSRIPRAENSNADALAKLAS 263
             +  ++E+S + R+ NS+AD  A +A+
Sbjct: 292 SDKCLSFEISHVLRSLNSDADEQANMAA 312

[Close]

BLAST of MC03g_new0106 vs. TAIR 10
Match: AT5G51080.3 (RNase H family protein )

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-06
Identity = 45/148 (30.41%), Postives = 73/148 (49.32%), Query Frame = 0


Query: 122 IAELTPPSELSESDLPWTVYVDGSS--NEKGCGAGVLLLGPGGERFEYALRFSFR----- 181
           +AEL P ++ S       +  DG+S  N    GA  +L    G     +L F  R     
Sbjct: 109 LAELEPSADTSYETC--IIEFDGASKGNPGLSGAAAVLKTEDG-----SLIFKMRQGLGI 168

Query: 182 TSNNEAEYEAFIAGLRIARTLGASCIKVFSDSQLVVSQIKDEYQAKDTRMEKYLGKVRSY 241
            +NN AEY   I GL+ A   G + IKV +DS+LV  Q+K +++     + K   + +  
Sbjct: 169 ATNNAAEYHGLILGLKHAIEKGYTKIKVKTDSKLVCMQMKGQWKVNHEVLSKLHKEAKQL 228

Query: 242 LAQFRTYEVSRIPRAENSNADALAKLAS 263
             +  ++E+S + R+ NS+AD  A +A+
Sbjct: 229 SDKCLSFEISHVLRSLNSDADEQANMAA 249

The following BLAST results are available for this feature:

BLAST of MC03g_new0106 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23074	2.4e-24	28.44	Pro-Pol polyprotein OS=Simian foamy virus type 1 OX=338478 GN=pol PE=1 SV=3	[more]
P27401	2.4e-24	28.13	Pro-Pol polyprotein OS=Simian foamy virus type 3 (strain LK3) OX=11644 GN=pol PE...	[more]
P03359	6.6e-19	27.04	Gag-Pol polyprotein OS=Woolly monkey sarcoma virus OX=11970 GN=pol PE=3 SV=2	[more]
Q9TTC1	8.7e-19	27.17	Gag-Pol polyprotein OS=Koala retrovirus OX=394239 GN=pro-pol PE=3 SV=2	[more]
P03360	1.9e-18	28.23	Gag-Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus OX=11636 GN=...	[more]

BLAST of MC03g_new0106 vs. NCBI nr


Match Name	Identity	Description
XP_022156575.1	97.54	uncharacterized protein LOC111023451 [Momordica charantia]	[more]
XP_022157799.1	85.58	uncharacterized protein LOC111024419 [Momordica charantia]	[more]
XP_022158579.1	62.01	uncharacterized protein LOC111025033 [Momordica charantia]	[more]
XP_022149417.1	77.98	protein NYNRIN-like [Momordica charantia]	[more]
XP_022143486.1	95.87	uncharacterized protein LOC111013362 [Momordica charantia]	[more]

BLAST of MC03g_new0106 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1DQP4	97.72	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023451 PE=4 SV=1	[more]
A0A6J1DXK8	85.58	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024419 PE=4 SV=1	[more]
A0A6J1DZU0	62.01	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025033 PE=4 SV=1	[more]
A0A6J1D7W6	77.98	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111017848 PE=4 SV=1	[more]
A0A6J1CQD7	95.87	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013362 PE=4 SV=1	[more]

BLAST of MC03g_new0106 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01410.1	8.3e-09	30.40	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G01410.2	8.3e-09	30.40	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G51080.1	3.0e-06	30.41	RNase H family protein	[more]
AT5G51080.2	3.0e-06	30.41	RNase H family protein	[more]
AT5G51080.3	3.0e-06	30.41	RNase H family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 607..627
None	No IPR available	GENE3D	1.10.340.70		coord: 312..403 e-value: 3.4E-16 score: 61.3
None	No IPR available	PANTHER	PTHR46387	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN	coord: 136..387
None	No IPR available	PANTHER	PTHR46387:SF6	14.7 KDA RIBONUCLEASE H-LIKE PROTEIN	coord: 136..387
None	No IPR available	CDD	cd09279	RNase_HI_like	coord: 138..262 e-value: 7.34385E-48 score: 163.027
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 413..617 e-value: 9.3E-55 score: 187.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 139..273 e-value: 1.5E-32 score: 114.4
IPR001584	Integrase, catalytic core	PFAM	PF00665	rve	coord: 422..518 e-value: 1.5E-14 score: 54.1
IPR001584	Integrase, catalytic core	PROSITE	PS50994	INTEGRASE	coord: 418..577 score: 26.059807
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 142..261 e-value: 4.9E-19 score: 68.4
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 134..263 score: 15.233902
IPR041588	Integrase zinc-binding domain	PFAM	PF17921	Integrase_H2C2	coord: 354..403 e-value: 1.3E-11 score: 44.4
IPR041373	Reverse transcriptase, RNase H-like domain	PFAM	PF17917	RT_RNaseH	coord: 3..103 e-value: 7.2E-19 score: 68.0
IPR043502	DNA/RNA polymerase superfamily	SUPERFAMILY	56672	DNA/RNA polymerases	coord: 3..103
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 137..259
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 418..571

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g_new0106.1	MC03g_new0106.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity