MC03g1317 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g1317
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	MC03: 18876932 .. 18878872 (+)
RNA-Seq Expression	MC03g1317
Synteny	MC03g1317

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGATAAGAAGTCAAGTATTTTCCTTCCTCTAATTAAAAGCTCACAAGAGTCTCACACCCCTACTTCAAATCAAAACATCAAAATCAAAAGAGAGAAAGTACTATTAAGTTCGAAGTGAAAATGGAGAACTCGAGAATTCCGACGGCGGATCTTTCTCCATTTTTGAGAAAAGAAGATGAAGATGAAGATGGAAGGAAGAAGGCGAGTGAGATTATTGGAAAAGCATGTTTGGAGTATGGATTCTTCCAGGTGGTGAACCATGGGGTGCCTGTTGAGCTTATGAAGAAAGCACTTTTGGTATCTAAGGAGTTTTTTGGTTACCCTTTGGAAAGAAAGCTTGAGGCCAGCCCTCTTCCCGGCGCACCACTGCCGGCGGGATATGGCCGCCAGCCGGAGCAATCTCCCGATAAGAACGAGTTCCTTATGATGTTTCCACCTGATTCAACCTTCAATGTCTTCCCCACCAATCCTCAATCCTTTAGGTTTTTCTTTAATATAATCTTCTCCTTCTCTAAGTTTAATTTCAATCTAACACTTTCTTTGTTCAGAGAATAAGCATGATGATATATATATATCTTGTTTGCTAATACAAGTAGCTTTGATATATTTGAAATGGTTTTTTAAATTCAGTCTCTGTCATTGTTTAAAAGTTTTCAATTTGGTCTTTTTTTTTTTTTTAACATATTTCAATTATACCATTACATTATTCTTATTATGTAGGCATAAACTTGGAAGGCTGGAGAAATTGGATCACCTAAACTAAGTTGAATTTTTTACCGCCTTATTATAGCTATAAATTTGGAGGAGAAATTAGAAACTTTATCCTTAAGTGGTCTAATTCAACTCACAAAATATTATGTTATTCTAAATTTATCATCAGTTAATTTTAGAACTTTTTAAATAATTGAGATTAAATTGAAAAAACACCTAATACATTATGGAATAAAATATATATAAAGAATTTATGACAAATATAAAAGGACTAAATGGAAAAAACAGCATGCACAATATACTTAGAACTCTTTTCTTCTTAGTATTTTCATACAAAGAAGACTGAACTGTTATGTGTTCTTCATCACTTGAGGATTGCAGCGAAGTTGTAAAGGAGCTATTCTCATACTTTGTGAAGACGGCTTCAATAGTAGAAAGTATCATAAACGACTGCTTGGGTCTTCCTCCAAACTTCCTCACTGAATACAATAACGACAGGTATATTCAATTGAACACAACATTTAATTGACCCATACTTAAAGAGTTTCTATAATCTTCTTCTAATTATTCATTAAGTTGATTGTTAATTCTGTTTAACAAATAGGAAGTGGGATCTGATGTCCGCATTCAGATACCCTCCTGCAACTGAGCTGGAAAACGTTGGACTTAGAGAGCATAAGGATGTTAACTGCATTACTTTTGTGTTCCAAGATGAAGTTGGAGGCCTCGAGCTCAAACGAGACGGCCGTTGGGTCCCGATCACTCCCGATCCCACCACCCTTGTTGTCAATGTAGGAGATGTCATTCAGGTAGTAATTAATATATTTGGTAACTGTTTCTTGTTTCTTGTTCTCTTTTTTGACTCGTACGTCTTGTATTGGCATAGGTACTGAGCAACGATAGGTGCAGGAGCGCGAGTCACAGGGTGGTGAGGCAGGCATGCAAAGAGCGTCATTCATATGCGTTCTTCTACAACATTGGAGGAGACAAGTGGGTGGAGCCATTGGCAGAGTTCACAAGGGAGATTAAAGAGCCACCAAAATACAGAGGCTTCATCTACAAGGACTACCTTCAATTAAGAATGAGAAACAAAACCCATCCTCCCTCTAAGCCTCAAGATGTCATCAATATAACTTATTATTCAACTTCCACCTGATTATCTCTCTTGATCCCCATATATTGTTCTTCCTCTTCAAACCCCATCAATAATACAATAATCTTTAATCAGT

mRNA sequence

CAGATAAGAAGTCAAGTATTTTCCTTCCTCTAATTAAAAGCTCACAAGAGTCTCACACCCCTACTTCAAATCAAAACATCAAAATCAAAAGAGAGAAAGTACTATTAAGTTCGAAGTGAAAATGGAGAACTCGAGAATTCCGACGGCGGATCTTTCTCCATTTTTGAGAAAAGAAGATGAAGATGAAGATGGAAGGAAGAAGGCGAGTGAGATTATTGGAAAAGCATGTTTGGAGTATGGATTCTTCCAGGTGGTGAACCATGGGGTGCCTGTTGAGCTTATGAAGAAAGCACTTTTGGTATCTAAGGAGTTTTTTGGTTACCCTTTGGAAAGAAAGCTTGAGGCCAGCCCTCTTCCCGGCGCACCACTGCCGGCGGGATATGGCCGCCAGCCGGAGCAATCTCCCGATAAGAACGAGTTCCTTATGATGTTTCCACCTGATTCAACCTTCAATGTCTTCCCCACCAATCCTCAATCCTTTAGCGAAGTTGTAAAGGAGCTATTCTCATACTTTGTGAAGACGGCTTCAATAGTAGAAAGTATCATAAACGACTGCTTGGGTCTTCCTCCAAACTTCCTCACTGAATACAATAACGACAGGAAGTGGGATCTGATGTCCGCATTCAGATACCCTCCTGCAACTGAGCTGGAAAACGTTGGACTTAGAGAGCATAAGGATGTTAACTGCATTACTTTTGTGTTCCAAGATGAAGTTGGAGGCCTCGAGCTCAAACGAGACGGCCGTTGGGTCCCGATCACTCCCGATCCCACCACCCTTGTTGTCAATGTAGGAGATGTCATTCAGGTACTGAGCAACGATAGGTGCAGGAGCGCGAGTCACAGGGTGGTGAGGCAGGCATGCAAAGAGCGTCATTCATATGCGTTCTTCTACAACATTGGAGGAGACAAGTGGGTGGAGCCATTGGCAGAGTTCACAAGGGAGATTAAAGAGCCACCAAAATACAGAGGCTTCATCTACAAGGACTACCTTCAATTAAGAATGAGAAACAAAACCCATCCTCCCTCTAAGCCTCAAGATGTCATCAATATAACTTATTATTCAACTTCCACCTGATTATCTCTCTTGATCCCCATATATTGTTCTTCCTCTTCAAACCCCATCAATAATACAATAATCTTTAATCAGT

Coding sequence (CDS)

ATGGAGAACTCGAGAATTCCGACGGCGGATCTTTCTCCATTTTTGAGAAAAGAAGATGAAGATGAAGATGGAAGGAAGAAGGCGAGTGAGATTATTGGAAAAGCATGTTTGGAGTATGGATTCTTCCAGGTGGTGAACCATGGGGTGCCTGTTGAGCTTATGAAGAAAGCACTTTTGGTATCTAAGGAGTTTTTTGGTTACCCTTTGGAAAGAAAGCTTGAGGCCAGCCCTCTTCCCGGCGCACCACTGCCGGCGGGATATGGCCGCCAGCCGGAGCAATCTCCCGATAAGAACGAGTTCCTTATGATGTTTCCACCTGATTCAACCTTCAATGTCTTCCCCACCAATCCTCAATCCTTTAGCGAAGTTGTAAAGGAGCTATTCTCATACTTTGTGAAGACGGCTTCAATAGTAGAAAGTATCATAAACGACTGCTTGGGTCTTCCTCCAAACTTCCTCACTGAATACAATAACGACAGGAAGTGGGATCTGATGTCCGCATTCAGATACCCTCCTGCAACTGAGCTGGAAAACGTTGGACTTAGAGAGCATAAGGATGTTAACTGCATTACTTTTGTGTTCCAAGATGAAGTTGGAGGCCTCGAGCTCAAACGAGACGGCCGTTGGGTCCCGATCACTCCCGATCCCACCACCCTTGTTGTCAATGTAGGAGATGTCATTCAGGTACTGAGCAACGATAGGTGCAGGAGCGCGAGTCACAGGGTGGTGAGGCAGGCATGCAAAGAGCGTCATTCATATGCGTTCTTCTACAACATTGGAGGAGACAAGTGGGTGGAGCCATTGGCAGAGTTCACAAGGGAGATTAAAGAGCCACCAAAATACAGAGGCTTCATCTACAAGGACTACCTTCAATTAAGAATGAGAAACAAAACCCATCCTCCCTCTAAGCCTCAAGATGTCATCAATATAACTTATTATTCAACTTCCACCTGA

Protein sequence

MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVGLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHPPSKPQDVINITYYSTST

Homology

[Close]

BLAST of MC03g1317 vs. ExPASy Swiss-Prot
Match: Q9FR99 (1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 95/289 (32.87%), Postives = 149/289 (51.56%), Query Frame = 0


Query: 23  DGRKKASEI--IGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGYPLERKLEASP--L 82
           DG+++A  +  I   C E+GFFQ+VNHG+PVEL+++   VS E +    ER   + P  L
Sbjct: 12  DGKERAETMARIANGCEEWGFFQLVNHGIPVELLERVKKVSSECYKLREERFEGSKPVQL 71

Query: 83  PGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSEVVKELFSYFVKTASIV 142
               +  G G++ +    ++ F++        N +P+NP  F E +KE      K A  +
Sbjct: 72  LDTLVKEGDGQRLDNVDWEDVFVL-----QDDNEWPSNPPDFEETMKEYREEIRKLAEKM 131

Query: 143 ESIINDCLGLPPNFLTE-YNNDRKWDLM---SAFRYPPATELENV-GLREHKDVNCITFV 202
             ++++ LG     + + ++ D +           YPP   L+ V GLR H D   +  +
Sbjct: 132 MEVMDENLGFEKGCIKKAFSGDGQHPPFFGTKVSHYPPCPRLDLVKGLRAHTDAGGVILL 191

Query: 203 FQ-DEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASHRVVRQACKERHS 262
           FQ D+VGGL++ +DGRW+ + P    +V+N GD I+VLSN R +SA HRV+  +   R S
Sbjct: 192 FQDDQVGGLQMLKDGRWIDVQPLADAIVINTGDQIEVLSNGRYKSAWHRVLATSHGNRRS 251

Query: 263 YAFFYNIGGDKWVEPLA-EFTREIKEPPKYRGFIYKDYLQLRMRNKTHP 301
            A FYN      + P A   T E   P  Y  F++ DY+ +  + K  P
Sbjct: 252 IASFYNPSLKATIAPAAGAATEEAAPPALYPKFLFGDYMDVYAKQKYEP 295

[Close]

BLAST of MC03g1317 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 92/292 (31.51%), Postives = 140/292 (47.95%), Query Frame = 0


Query: 8   TADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGY 67
           + D  P +   D     R         AC   GFFQ+ NHGVP E +KK +  ++EFF  
Sbjct: 38  SGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 97

Query: 68  PLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTFNVFPTNPQSFSE 127
               +++   A       L   +    E+  +  +FL +  +P +   N +P+ P SF E
Sbjct: 98  SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 157

Query: 128 VVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYP--PATELENVG 187
           V  E  +        +   I++ LGL  + ++     +    M+   YP  P  EL   G
Sbjct: 158 VTAEYATSVRALVLTLLEAISESLGLAKDRVSN-TIGKHGQHMAINYYPRCPQPEL-TYG 217

Query: 188 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH 247
           L  HKD N IT + QDEV GL++ +DG+W+ + P P T +VN+GD +QV+SN++ +S  H
Sbjct: 218 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 277

Query: 248 RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPK-YRGFIYKDYLQ 292
           R V  +  ER S   FY    D  + P  E   E ++ P  YR F Y +Y +
Sbjct: 278 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFE 327

[Close]

BLAST of MC03g1317 vs. ExPASy Swiss-Prot
Match: O04395 (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.1e-32
Identity = 91/305 (29.84%), Postives = 154/305 (50.49%), Query Frame = 0


Query: 5   RIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEF 64
           ++P  DLS        DE+     +  + KA  ++G FQVVNHG+P EL+++   V +EF
Sbjct: 1   QVPVVDLS------CPDEE---LVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREF 60

Query: 65  FGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNE------FLMMFPPDS-TFNVFPTNP 124
           F  P   K   +   G+    GYGR+ E    K +      +L  +PP S  +  +P +P
Sbjct: 61  FELPEAEKRSCAREAGS--VEGYGRRIELDIKKRKGIVDQIYLSTWPPSSVNYRYWPKSP 120

Query: 125 QSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELE 184
             + EV +E   +    +  +   +++ LGL    + E N    W +M+   YPP    +
Sbjct: 121 PDYREVNEEYARHVKTLSEKIMEWLSEGLGLGREAIKEVNG--CWYVMNINHYPPYPHSD 180

Query: 185 NV-GLREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCR 244
           +  GL  H D+N +T +  +E+ GL++ +D  W+ +   P+ ++VN+GD I +LSN + +
Sbjct: 181 SFNGLEPHTDINGLTLIITNEIPGLQVFKDDHWIEVEYIPSAIIVNIGDQIMMLSNGKYK 240

Query: 245 SASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLR--- 299
           +  H+      K R S+    +   D  V PL E T E  +PPK++   YKDY+  +   
Sbjct: 241 NVLHKTTVDKEKTRMSWPVLVSPTYDMVVGPLPELTSE-DDPPKFKPIAYKDYVHNKITF 291

[Close]

BLAST of MC03g1317 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 3.1e-32
Identity = 88/288 (30.56%), Postives = 136/288 (47.22%), Query Frame = 0


Query: 8   TADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGY 67
           + D  P +   D     R    + +  AC  YGFFQ+ NHGVP   + K   V++EFF  
Sbjct: 40  SGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQ 99

Query: 68  PLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTFNVFPTNPQSFSE 127
           P   +++   A P     L   +    ++  +  +FL +  FP +     +P++P SF E
Sbjct: 100 PESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCFPIEDFIEEWPSSPISFRE 159

Query: 128 VVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELE-NVGL 187
           V  E  +        +   I++ LGL  + ++         +   + YPP  E E   GL
Sbjct: 160 VTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNY-YPPCPEPELTYGL 219

Query: 188 REHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASHR 247
             HKD   IT + QD+V GL++ +D +WV ++P P T +VN+GD +QV+SND+ +S  HR
Sbjct: 220 PGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHR 279

Query: 248 VVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDY 290
            V     ER S   FY    D  + P  E   E      YR + + +Y
Sbjct: 280 AVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEY 326

[Close]

BLAST of MC03g1317 vs. ExPASy Swiss-Prot
Match: Q0WPW4 (1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g77330 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.0e-32
Identity = 99/307 (32.25%), Postives = 149/307 (48.53%), Query Frame = 0


Query: 6   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 65
           IP  D S    +E      R+K    I +AC E+GFFQ+VNHG+P+EL+ K   +S + +
Sbjct: 3   IPVIDFSKLNGEE------REKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCY 62

Query: 66  GYPLERKLEAS---PLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSE 125
               E   + S    L    +    G + E    ++ F ++   D   N +P+N     E
Sbjct: 63  KTEREEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEWPSN---IKE 122

Query: 126 VVKELFSYFVKTASIVESIINDCLGLPPNFLTE-YNNDRKWDLMSAF------RYPPATE 185
            + E      K AS +  ++++ LGLP  ++ + +N   +    +AF       YPP   
Sbjct: 123 TMGEYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPH 182

Query: 186 LENV-GLREHKDVNCITFVFQ-DEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSND 245
            E V GLR H D   +  +FQ DE  GL++ +DG W+ + P P  +V+N GD I+VLSN 
Sbjct: 183 PELVNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNG 242

Query: 246 RCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLR 301
           R +SA HRV+ +    R S A FYN      + P A    E  E  KY  F++ DY+ + 
Sbjct: 243 RYKSAWHRVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGSE-KKYPKFVFGDYMDVY 296

[Close]

BLAST of MC03g1317 vs. NCBI nr
Match: XP_022137076.1 (1-aminocyclopropane-1-carboxylate oxidase-like [Momordica charantia])

HSP 1 Score: 656 bits (1692), Expect = 6.37e-238
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV
Sbjct: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF
Sbjct: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG
Sbjct: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH 240
           LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH
Sbjct: 181 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH 240

Query: 241 RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHP 300
           RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHP
Sbjct: 241 RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHP 300

Query: 301 PSKPQDVINITYYSTST 317
           PSKPQDVINITYYSTST
Sbjct: 301 PSKPQDVINITYYSTST 317

[Close]

BLAST of MC03g1317 vs. NCBI nr
Match: XP_022923754.1 (1-aminocyclopropane-1-carboxylate oxidase 1-like [Cucurbita moschata])

HSP 1 Score: 532 bits (1371), Expect = 5.33e-189
Identity = 258/318 (81.13%), Postives = 284/318 (89.31%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           M N+ IPTADLSPF  +E  DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNAGIPTADLSPFFSEEG-DEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYPL+RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDST NVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTLNVFPSNPQGF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVLSNDRCRSAS 240
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+QVLSNDR +SAS
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQVLSNDRYKSAS 240

Query: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300
           HRVVRQA +ERHS+AFFYNIGGDKWVEPL  F+ +I E PKYRGF+YKDYLQLR+RNKTH
Sbjct: 241 HRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKTH 300

Query: 301 PPSKPQDVINITYYSTST 317
           PPS+PQD+INI+YYSTST
Sbjct: 301 PPSRPQDIINISYYSTST 317

[Close]

BLAST of MC03g1317 vs. NCBI nr
Match: XP_023001093.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 530 bits (1364), Expect = 6.20e-188
Identity = 256/318 (80.50%), Postives = 282/318 (88.68%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           M NS IPTADLSPF  +E  DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNSGIPTADLSPFFTEEG-DEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGG 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYP +RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDSTFNVFP+NPQ F
Sbjct: 61  SKEFFGYPSDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTFNVFPSNPQGF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           S V+KELFS FV TASIVESIINDCLGLPPNFL EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVNTASIVESIINDCLGLPPNFLGEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVLSNDRCRSAS 240
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTL+VNVGDV+QVLSNDR +SA 
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKADVDQWIPITPDRTTLIVNVGDVVQVLSNDRYKSAG 240

Query: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300
           HRVVRQA +ERHS+AFFYNIGGDKWVEPL  F+ +I EPPKYRGF+Y+DYLQLR+RNKTH
Sbjct: 241 HRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEPPKYRGFLYQDYLQLRLRNKTH 300

Query: 301 PPSKPQDVINITYYSTST 317
           PPS+PQD+INI+YYSTST
Sbjct: 301 PPSRPQDIINISYYSTST 317

[Close]

BLAST of MC03g1317 vs. NCBI nr
Match: KAG7020043.1 (1-aminocyclopropane-1-carboxylate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 527 bits (1358), Expect = 5.09e-187
Identity = 256/318 (80.50%), Postives = 283/318 (88.99%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           M N+ IPTADLSPF  +E  DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNAGIPTADLSPFFSEEG-DEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYPL+RKLEASP PGAPLPAGYGRQP  S DKNEF +MFPPDST NVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPGAPLPAGYGRQPPHSSDKNEFFLMFPPDSTLNVFPSNPQGF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYN+DRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNSDRKWDLMSTFRYPCASEVENIG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVLSNDRCRSAS 240
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+QVLSNDR +SAS
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQVLSNDRYKSAS 240

Query: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300
           HRVVRQA +ERHS+AFFYNIGGDKWVEPL  F+ +I E PKYRGF+YKDYLQLR+RNKTH
Sbjct: 241 HRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKTH 300

Query: 301 PPSKPQDVINITYYSTST 317
           PPS+PQD+INI+YYSTST
Sbjct: 301 PPSRPQDIINISYYSTST 317

[Close]

BLAST of MC03g1317 vs. NCBI nr
Match: XP_023520152.1 (probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527 bits (1357), Expect = 6.97e-187
Identity = 255/318 (80.19%), Postives = 283/318 (88.99%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           M NS IPTADLSPF  +   DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNSGIPTADLSPFFTEG--DEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYPL+RKLEASP P APLPAGYGR P  SPDKNEF +MFPPDSTFNVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPSAPLPAGYGRPPPHSPDKNEFFLMFPPDSTFNVFPSNPQGF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYNNDR+WDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNNDRRWDLMSTFRYPCASEVENIG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVLSNDRCRSAS 240
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+QVLSNDR +SAS
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQVLSNDRYKSAS 240

Query: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300
           HRVVRQA +ERHS+AFFYNIGGDKWVEPL +F+ +I E PKYRGF+YK+YLQLR+RNKTH
Sbjct: 241 HRVVRQAGRERHSFAFFYNIGGDKWVEPLPDFSTDIDEAPKYRGFLYKEYLQLRLRNKTH 300

Query: 301 PPSKPQDVINITYYSTST 317
           PPS+PQD+INI+YYSTST
Sbjct: 301 PPSRPQDIINISYYSTST 316

[Close]

BLAST of MC03g1317 vs. ExPASy TrEMBL
Match: A0A6J1C5F5 (1-aminocyclopropane-1-carboxylate oxidase-like OS=Momordica charantia OX=3673 GN=LOC111008638 PE=3 SV=1)

HSP 1 Score: 656 bits (1692), Expect = 3.08e-238
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV
Sbjct: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF
Sbjct: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG
Sbjct: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH 240
           LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH
Sbjct: 181 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH 240

Query: 241 RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHP 300
           RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHP
Sbjct: 241 RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTHP 300

Query: 301 PSKPQDVINITYYSTST 317
           PSKPQDVINITYYSTST
Sbjct: 301 PSKPQDVINITYYSTST 317

[Close]

BLAST of MC03g1317 vs. ExPASy TrEMBL
Match: A0A6J1E7A1 (1-aminocyclopropane-1-carboxylate oxidase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431366 PE=3 SV=1)

HSP 1 Score: 532 bits (1371), Expect = 2.58e-189
Identity = 258/318 (81.13%), Postives = 284/318 (89.31%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           M N+ IPTADLSPF  +E  DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNAGIPTADLSPFFSEEG-DEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGR 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYPL+RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDST NVFP+NPQ F
Sbjct: 61  SKEFFGYPLDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTLNVFPSNPQGF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           S V+KELFS FVKTASIVESIINDCLGLPPNFL+EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVKTASIVESIINDCLGLPPNFLSEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVLSNDRCRSAS 240
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTLVVNVGDV+QVLSNDR +SAS
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKTDVDQWIPITPDQTTLVVNVGDVVQVLSNDRYKSAS 240

Query: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300
           HRVVRQA +ERHS+AFFYNIGGDKWVEPL  F+ +I E PKYRGF+YKDYLQLR+RNKTH
Sbjct: 241 HRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEAPKYRGFLYKDYLQLRLRNKTH 300

Query: 301 PPSKPQDVINITYYSTST 317
           PPS+PQD+INI+YYSTST
Sbjct: 301 PPSRPQDIINISYYSTST 317

[Close]

BLAST of MC03g1317 vs. ExPASy TrEMBL
Match: A0A6J1KPI6 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111495340 PE=3 SV=1)

HSP 1 Score: 530 bits (1364), Expect = 3.00e-188
Identity = 256/318 (80.50%), Postives = 282/318 (88.68%), Query Frame = 0


Query: 1   MENSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLV 60
           M NS IPTADLSPF  +E  DE G+KKA EIIGKAC + GFFQVVNHGVPVELM KAL  
Sbjct: 1   MVNSGIPTADLSPFFTEEG-DEGGKKKAIEIIGKACSDLGFFQVVNHGVPVELMAKALGG 60

Query: 61  SKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSF 120
           SKEFFGYP +RKLEASP PGAPLPAGYGRQP  SPDKNEF +MFPPDSTFNVFP+NPQ F
Sbjct: 61  SKEFFGYPSDRKLEASPQPGAPLPAGYGRQPPHSPDKNEFFLMFPPDSTFNVFPSNPQGF 120

Query: 121 SEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELENVG 180
           S V+KELFS FV TASIVESIINDCLGLPPNFL EYNNDRKWDLMS FRYP A+E+EN+G
Sbjct: 121 SCVLKELFSSFVNTASIVESIINDCLGLPPNFLGEYNNDRKWDLMSTFRYPCASEVENIG 180

Query: 181 LREHKDVNCITFVFQDEVGGLELKRD-GRWVPITPDPTTLVVNVGDVIQVLSNDRCRSAS 240
           +REHKDVNCITFVFQDEVGGLELK D  +W+PITPD TTL+VNVGDV+QVLSNDR +SA 
Sbjct: 181 VREHKDVNCITFVFQDEVGGLELKADVDQWIPITPDRTTLIVNVGDVVQVLSNDRYKSAG 240

Query: 241 HRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLRMRNKTH 300
           HRVVRQA +ERHS+AFFYNIGGDKWVEPL  F+ +I EPPKYRGF+Y+DYLQLR+RNKTH
Sbjct: 241 HRVVRQAGRERHSFAFFYNIGGDKWVEPLPHFSTDIDEPPKYRGFLYQDYLQLRLRNKTH 300

Query: 301 PPSKPQDVINITYYSTST 317
           PPS+PQD+INI+YYSTST
Sbjct: 301 PPSRPQDIINISYYSTST 317

[Close]

BLAST of MC03g1317 vs. ExPASy TrEMBL
Match: S0BE26 (GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox2 PE=2 SV=1)

HSP 1 Score: 499 bits (1284), Expect = 7.61e-176
Identity = 239/327 (73.09%), Postives = 280/327 (85.63%), Query Frame = 0


Query: 2   ENSRIPTADLSPFLRK--------EDEDEDGRKK---ASEIIGKACLEYGFFQVVNHGVP 61
           E   IPTADLSPFL++        +D+D+D +KK   A EIIGKAC E+GFFQVVNHGVP
Sbjct: 5   EEITIPTADLSPFLKELDQGSYSYDDDDDDQKKKKAAAIEIIGKACSEFGFFQVVNHGVP 64

Query: 62  VELMKKALLVSKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTF 121
           + LM+KALL+S +FFGYPL+RKL+ASPLPGAP+PAGYGRQP+ SPDKNEF MMFPP STF
Sbjct: 65  LHLMQKALLLSNQFFGYPLDRKLQASPLPGAPMPAGYGRQPDHSPDKNEFFMMFPPHSTF 124

Query: 122 NVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRY 181
           NVFP++PQ F EVV+ELFS FVKTAS++E+IIN+CLGLPPNFL+EYNNDRKWDLMS FRY
Sbjct: 125 NVFPSHPQGFREVVEELFSCFVKTASVIENIINECLGLPPNFLSEYNNDRKWDLMSTFRY 184

Query: 182 PPATELENVGLREHKDVNCITFVFQDEVGGLELK-RDGRWVPITPDPTTLVVNVGDVIQV 241
           P A+E+ENVGLREHKDVN IT +FQDEVGGLE+K  D +W+PI P+  TLV+NVGDVIQV
Sbjct: 185 PNASEIENVGLREHKDVNFITLLFQDEVGGLEVKTEDHQWIPIIPNQNTLVINVGDVIQV 244

Query: 242 LSNDRCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDY 301
           LSNDR +SASHRVVRQ  +ERHSYAFFYNIGGDK V+PL  FT  I +PP Y+ FIYK+Y
Sbjct: 245 LSNDRYKSASHRVVRQEGRERHSYAFFYNIGGDKLVQPLPHFTTHIDQPPNYKSFIYKEY 304

Query: 302 LQLRMRNKTHPPSKPQDVINITYYSTS 316
           LQLR+RNKTHPPS PQD+INI+YYST+
Sbjct: 305 LQLRLRNKTHPPSNPQDIINISYYSTT 331

[Close]

BLAST of MC03g1317 vs. ExPASy TrEMBL
Match: A0A1S3B2U8 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103485123 PE=3 SV=1)

HSP 1 Score: 494 bits (1273), Expect = 3.87e-174
Identity = 238/325 (73.23%), Postives = 280/325 (86.15%), Query Frame = 0


Query: 6   IPTADLSPFLRK----------EDEDEDGRKK---ASEIIGKACLEYGFFQVVNHGVPVE 65
           IPTADLSPFL++          +DED+  +KK   A EIIGKAC E+GFFQVVNHGVP+ 
Sbjct: 9   IPTADLSPFLKELEESAYSYDADDEDDQKKKKKKDAIEIIGKACSEFGFFQVVNHGVPLH 68

Query: 66  LMKKALLVSKEFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNV 125
           LM+KALL+S +FFGYPL+RKL+ASPL GAPLPAGYGRQP++SPDKNEF MMFPP STFNV
Sbjct: 69  LMQKALLLSNQFFGYPLDRKLQASPLTGAPLPAGYGRQPDRSPDKNEFFMMFPPHSTFNV 128

Query: 126 FPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPP 185
           FP++PQ F EVV+ELFS FVKTASI+E+IIN+CLGLPPNFL+EYNNDRKWDLMS FRYP 
Sbjct: 129 FPSDPQGFREVVEELFSCFVKTASIIENIINECLGLPPNFLSEYNNDRKWDLMSTFRYPN 188

Query: 186 ATELENVGLREHKDVNCITFVFQDEVGGLELK-RDGRWVPITPDPTTLVVNVGDVIQVLS 245
           A+E+ENVGLREHKDVN IT +FQDEVGGLE+K  D +W+PI P+  +LV+NVGDVIQVLS
Sbjct: 189 ASEIENVGLREHKDVNFITLLFQDEVGGLEVKTEDHQWIPIIPNQNSLVINVGDVIQVLS 248

Query: 246 NDRCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQ 305
           NDR +SASHRVVRQA +ERHSYAFFYNIGGDK V+PL  FT  I +PPKY+ FIYK+YLQ
Sbjct: 249 NDRYKSASHRVVRQAGRERHSYAFFYNIGGDKLVQPLPHFTTHIDQPPKYKSFIYKEYLQ 308

Query: 306 LRMRNKTHPPSKPQDVINITYYSTS 316
           LR+RNKTHPP+ P+D+INI+YYST+
Sbjct: 309 LRLRNKTHPPTNPKDIINISYYSTT 333

[Close]

BLAST of MC03g1317 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 153.7 bits (387), Expect = 2.5e-37
Identity = 104/313 (33.23%), Postives = 157/313 (50.16%), Query Frame = 0


Query: 6   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 65
           IP  DLS    + D+ ED +   SE IG AC ++GFFQV+NHGVP +  ++     K FF
Sbjct: 28  IPVIDLS----RLDDPEDVQNVISE-IGDACEKWGFFQVLNHGVPSDARQRVEKTVKMFF 87

Query: 66  GYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMF------------PPDS----T 125
             P+E K++       P+    G   +   D  E   ++            P D      
Sbjct: 88  DLPMEEKIKVKRDDVNPVGYHDGEHTKNVKDWKEVFDIYFKDPMVIPSTTDPEDEGLRLV 147

Query: 126 FNVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAF- 185
           +N +P +P  F E  +    +  K A  +  +I+  LGLP     +Y  ++    MS F 
Sbjct: 148 YNKWPQSPSDFREACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQ----MSFFR 207

Query: 186 --RYPPATELE-NVGLREHKDVNCITFVFQDEVGGLELKR--DGRWVPITPDPTTLVVNV 245
             RYPP    +  +G+  HKD + I+ + QD+VGGL++ R  DG W PI P P  LV+N+
Sbjct: 208 INRYPPCPRPDLALGVGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINI 267

Query: 246 GDVIQVLSNDRCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRG 297
           G+ +++ +ND+  SA HRVV    +ER+S  FF     D  V+PL E     + PPKY+G
Sbjct: 268 GNCMEIWTNDKYWSAEHRVVVNTTRERYSIPFFLLPSHDVEVKPLEELVSP-ENPPKYKG 327

[Close]

BLAST of MC03g1317 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 5.6e-37
Identity = 104/313 (33.23%), Postives = 148/313 (47.28%), Query Frame = 0


Query: 3   NSRIPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSK 62
           +  IPT DLS     ED   D    A E I +AC  +GFFQV+NHG+P  L  +    + 
Sbjct: 29  SDEIPTIDLSSL---EDTHHDKTAIAKE-IAEACKRWGFFQVINHGLPSALRHRVEKTAA 88

Query: 63  EFFGYPLERKLEASPLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTF------------ 122
           EFF    E K +       P+        +   D  E    F  DST             
Sbjct: 89  EFFNLTTEEKRKVKRDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTEL 148

Query: 123 ----NVFPTNPQSFSEVVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMS 182
               N +P NP  F EV +E      K A  +  +++  LGLP + LT + N++    + 
Sbjct: 149 RKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQT-SFLR 208

Query: 183 AFRYPPATELE-NVGLREHKDVNCITFVFQDEVGGLELKR--DGRWVPITPDPTTLVVNV 242
              YPP    E  +G+  HKD   +T + QD VGGL++ R  DG+W+P+ P    L++N+
Sbjct: 209 FNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINM 268

Query: 243 GDVIQVLSNDRCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRG 297
           G+ IQV +ND   SA HRVV    KER S  FF+    +  +EPL E   E + PP Y+ 
Sbjct: 269 GNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISE-ENPPCYKK 328

[Close]

BLAST of MC03g1317 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-34
Identity = 92/292 (31.51%), Postives = 140/292 (47.95%), Query Frame = 0


Query: 8   TADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGY 67
           + D  P +   D     R         AC   GFFQ+ NHGVP E +KK +  ++EFF  
Sbjct: 38  SGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 97

Query: 68  PLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTFNVFPTNPQSFSE 127
               +++   A       L   +    E+  +  +FL +  +P +   N +P+ P SF E
Sbjct: 98  SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 157

Query: 128 VVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYP--PATELENVG 187
           V  E  +        +   I++ LGL  + ++     +    M+   YP  P  EL   G
Sbjct: 158 VTAEYATSVRALVLTLLEAISESLGLAKDRVSN-TIGKHGQHMAINYYPRCPQPEL-TYG 217

Query: 188 LREHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASH 247
           L  HKD N IT + QDEV GL++ +DG+W+ + P P T +VN+GD +QV+SN++ +S  H
Sbjct: 218 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 277

Query: 248 RVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPK-YRGFIYKDYLQ 292
           R V  +  ER S   FY    D  + P  E   E ++ P  YR F Y +Y +
Sbjct: 278 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFE 327

[Close]

BLAST of MC03g1317 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 2.2e-33
Identity = 88/288 (30.56%), Postives = 136/288 (47.22%), Query Frame = 0


Query: 8   TADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFFGY 67
           + D  P +   D     R    + +  AC  YGFFQ+ NHGVP   + K   V++EFF  
Sbjct: 40  SGDSIPLIDLRDLHGPNRAVIVQQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQ 99

Query: 68  PLERKLE---ASPLPGAPLPAGYGRQPEQSPDKNEFLMM--FPPDSTFNVFPTNPQSFSE 127
           P   +++   A P     L   +    ++  +  +FL +  FP +     +P++P SF E
Sbjct: 100 PESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCFPIEDFIEEWPSSPISFRE 159

Query: 128 VVKELFSYFVKTASIVESIINDCLGLPPNFLTEYNNDRKWDLMSAFRYPPATELE-NVGL 187
           V  E  +        +   I++ LGL  + ++         +   + YPP  E E   GL
Sbjct: 160 VTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNY-YPPCPEPELTYGL 219

Query: 188 REHKDVNCITFVFQDEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSNDRCRSASHR 247
             HKD   IT + QD+V GL++ +D +WV ++P P T +VN+GD +QV+SND+ +S  HR
Sbjct: 220 PGHKDPTVITVLLQDQVSGLQVFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHR 279

Query: 248 VVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDY 290
            V     ER S   FY    D  + P  E   E      YR + + +Y
Sbjct: 280 AVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEY 326

[Close]

BLAST of MC03g1317 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 140.2 bits (352), Expect = 2.9e-33
Identity = 99/307 (32.25%), Postives = 149/307 (48.53%), Query Frame = 0


Query: 6   IPTADLSPFLRKEDEDEDGRKKASEIIGKACLEYGFFQVVNHGVPVELMKKALLVSKEFF 65
           IP  D S    +E      R+K    I +AC E+GFFQ+VNHG+P+EL+ K   +S + +
Sbjct: 3   IPVIDFSKLNGEE------REKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCY 62

Query: 66  GYPLERKLEAS---PLPGAPLPAGYGRQPEQSPDKNEFLMMFPPDSTFNVFPTNPQSFSE 125
               E   + S    L    +    G + E    ++ F ++   D   N +P+N     E
Sbjct: 63  KTEREEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTLL---DHNQNEWPSN---IKE 122

Query: 126 VVKELFSYFVKTASIVESIINDCLGLPPNFLTE-YNNDRKWDLMSAF------RYPPATE 185
            + E      K AS +  ++++ LGLP  ++ + +N   +    +AF       YPP   
Sbjct: 123 TMGEYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPH 182

Query: 186 LENV-GLREHKDVNCITFVFQ-DEVGGLELKRDGRWVPITPDPTTLVVNVGDVIQVLSND 245
            E V GLR H D   +  +FQ DE  GL++ +DG W+ + P P  +V+N GD I+VLSN 
Sbjct: 183 PELVNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLPNAIVINTGDQIEVLSNG 242

Query: 246 RCRSASHRVVRQACKERHSYAFFYNIGGDKWVEPLAEFTREIKEPPKYRGFIYKDYLQLR 301
           R +SA HRV+ +    R S A FYN      + P A    E  E  KY  F++ DY+ + 
Sbjct: 243 RYKSAWHRVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGSE-KKYPKFVFGDYMDVY 296

The following BLAST results are available for this feature:

BLAST of MC03g1317 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FR99	1.5e-34	32.87	1-aminocyclopropane-1-carboxylate oxidase OS=Musa acuminata OX=4641 GN=MAO1B PE=...	[more]
Q9ZSA7	2.1e-33	31.51	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
O04395	1.1e-32	29.84	Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724...	[more]
Q9ZSA8	3.1e-32	30.56	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]
Q0WPW4	4.0e-32	32.25	1-aminocyclopropane-1-carboxylate oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]

BLAST of MC03g1317 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137076.1	6.37e-238	100.00	1-aminocyclopropane-1-carboxylate oxidase-like [Momordica charantia]	[more]
XP_022923754.1	5.33e-189	81.13	1-aminocyclopropane-1-carboxylate oxidase 1-like [Cucurbita moschata]	[more]
XP_023001093.1	6.20e-188	80.50	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima]	[more]
KAG7020043.1	5.09e-187	80.50	1-aminocyclopropane-1-carboxylate oxidase, partial [Cucurbita argyrosperma subsp...	[more]
XP_023520152.1	6.97e-187	80.19	probable 2-oxoglutarate-dependent dioxygenase ANS isoform X1 [Cucurbita pepo sub...	[more]

BLAST of MC03g1317 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5F5	3.08e-238	100.00	1-aminocyclopropane-1-carboxylate oxidase-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1E7A1	2.58e-189	81.13	1-aminocyclopropane-1-carboxylate oxidase 1-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1KPI6	3.00e-188	80.50	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
S0BE26	7.61e-176	73.09	GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox2 PE=2 SV=1	[more]
A0A1S3B2U8	3.87e-174	73.23	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of MC03g1317 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19010.1	2.5e-37	33.23	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	5.6e-37	33.23	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	1.5e-34	31.51	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10500.1	2.2e-33	30.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G77330.1	2.9e-33	32.25	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 165..258 e-value: 1.4E-20 score: 73.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..100 e-value: 1.0E-18 score: 68.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..300 e-value: 8.4E-79 score: 267.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	PANTHER	PTHR10209:SF732	FLAVONOL SYNTHASE/FLAVANONE 3-HYDROXYLASE-LIKE	coord: 1..315
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..315
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..297
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..259 score: 11.353998

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g1317.1	MC03g1317.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding