MC03g1216 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g1216
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	protein ASPARTIC PROTEASE IN GUARD CELL 1-like
Location	MC03: 18195103 .. 18196557 (-)
RNA-Seq Expression	MC03g1216
Synteny	MC03g1216

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGCTACCTGTGTTCACCATCTTCATTTTCTCTCTCTTCTTGCCCTTCACATCATGCCGCAGTTCGCCGGAGCTTTCTCGCTCCTCCTCCGCTCTGCTCGATGTTTCAGCTTCACTGAAACAAGCCGACCAGGTCCTGAAATTCGATCCCAAGGGTTTAATGTCTTTCCAGCAGCAGGAGCAGCTTGTTCCGGCGAACTCTTCCTCCTTCTCTCTGCAACTGCACCCTCGCGATGCTCTTCGCAATGCCGGACACAAGGACTACAAGAGCCTCGTCCTCTCACGCCTCGACCGCGACTCCTCTCGAGTTAAATCGCTCAACGACAGGCTTCAATTTGCCCTCAGTGACCTGAAGAGGTCGGACCTTCAGCCGTTGAAGACGGAGATTCTGCCGGAGGATCTGAGCACTCCGATTGTCTCCGGCTCCAGTCAGGGCAGCGGCGAGTATTTCTCCCGCGTCGGCGTCGGACAACCGGCCAAGCCATTCTACATGGTTCTCGATACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGCTACCAGCAATCCGACCCGATTTTTGACCCGAGAACTTCCTCCTCTTTTGCTTCCCTTCCCTGCGAATCTCAGCAATGCCAAGCTCTAGAAATGTCTGGTTGTCGTGCTGCCAAGTGTCTTTACCAGGTCTCGTATGGCGATGGCTCCTTCACCGTCGGCGAGTTCGTCACTGAAACGCTGTCGTTCGGCAACTCCGGGACGATTAACAACGTCGCTCTCGGGTGCGGCCACGACAACGAGGGACTGTTCGTCGGATCCGCTGGATTGCTCGGACTCGGCGGCGGATCACTATCCCTCACTTCTCAGATGAAAGCGTCGTCGTTTTCGTACTGTCTTGTGGACCGCGACTCTGGTTCGTCTTCGAGCCTGGATTTCAATTCCGGCGCACCCAGTGACTCAGTGACTGCTCCATTGCTCAGAAGCGGAAGAGTCGATACTTTCTACTACGTCGGGCTGACCGGACTGAGCGTCGGCGGGCAGTTGCTGTCGATTCCACCGTCTCTGTTCCAAATGGACGATTCCGGGTACGGCGGAATCATCGTGGACTCTGGAACCGCCATAACTCGGCTGCAGACTCAGGCGTACAACTCCCTCCGCGATGCCTTCGTGAGGCTGACGCCGTACCTGAGGAAGACGAACGGGTTCGCACTATTCGACACGTGTTACGATCTGTCGTCCCAGTCGAGAGTAACGATTCCGACGGTGTCGTTCCACTTCTCCGGCGGGAAGTCCCTGATGCTGCCGCCGAAGAACTACCTGATCCCGGTGGACTCAGCCGGAACTTTCTGCTTTGCGTTCGCCCCAACGACGTCGTCTCTGTCGATCATAGGGAACGTTCAGCAGCAGGGGACACGTGTCAACTTCGATTTGGCAAACTCGCTCGTAGGGTTTTCACCCAATAAATGT

mRNA sequence

GTGCTACCTGTGTTCACCATCTTCATTTTCTCTCTCTTCTTGCCCTTCACATCATGCCGCAGTTCGCCGGAGCTTTCTCGCTCCTCCTCCGCTCTGCTCGATGTTTCAGCTTCACTGAAACAAGCCGACCAGGTCCTGAAATTCGATCCCAAGGGTTTAATGTCTTTCCAGCAGCAGGAGCAGCTTGTTCCGGCGAACTCTTCCTCCTTCTCTCTGCAACTGCACCCTCGCGATGCTCTTCGCAATGCCGGACACAAGGACTACAAGAGCCTCGTCCTCTCACGCCTCGACCGCGACTCCTCTCGAGTTAAATCGCTCAACGACAGGCTTCAATTTGCCCTCAGTGACCTGAAGAGGTCGGACCTTCAGCCGTTGAAGACGGAGATTCTGCCGGAGGATCTGAGCACTCCGATTGTCTCCGGCTCCAGTCAGGGCAGCGGCGAGTATTTCTCCCGCGTCGGCGTCGGACAACCGGCCAAGCCATTCTACATGGTTCTCGATACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGCTACCAGCAATCCGACCCGATTTTTGACCCGAGAACTTCCTCCTCTTTTGCTTCCCTTCCCTGCGAATCTCAGCAATGCCAAGCTCTAGAAATGTCTGGTTGTCGTGCTGCCAAGTGTCTTTACCAGGTCTCGTATGGCGATGGCTCCTTCACCGTCGGCGAGTTCGTCACTGAAACGCTGTCGTTCGGCAACTCCGGGACGATTAACAACGTCGCTCTCGGGTGCGGCCACGACAACGAGGGACTGTTCGTCGGATCCGCTGGATTGCTCGGACTCGGCGGCGGATCACTATCCCTCACTTCTCAGATGAAAGCGTCGTCGTTTTCGTACTGTCTTGTGGACCGCGACTCTGGTTCGTCTTCGAGCCTGGATTTCAATTCCGGCGCACCCAGTGACTCAGTGACTGCTCCATTGCTCAGAAGCGGAAGAGTCGATACTTTCTACTACGTCGGGCTGACCGGACTGAGCGTCGGCGGGCAGTTGCTGTCGATTCCACCGTCTCTGTTCCAAATGGACGATTCCGGGTACGGCGGAATCATCGTGGACTCTGGAACCGCCATAACTCGGCTGCAGACTCAGGCGTACAACTCCCTCCGCGATGCCTTCGTGAGGCTGACGCCGTACCTGAGGAAGACGAACGGGTTCGCACTATTCGACACGTGTTACGATCTGTCGTCCCAGTCGAGAGTAACGATTCCGACGGTGTCGTTCCACTTCTCCGGCGGGAAGTCCCTGATGCTGCCGCCGAAGAACTACCTGATCCCGGTGGACTCAGCCGGAACTTTCTGCTTTGCGTTCGCCCCAACGACGTCGTCTCTGTCGATCATAGGGAACGTTCAGCAGCAGGGGACACGTGTCAACTTCGATTTGGCAAACTCGCTCGTAGGGTTTTCACCCAATAAATGT

Coding sequence (CDS)

GTGCTACCTGTGTTCACCATCTTCATTTTCTCTCTCTTCTTGCCCTTCACATCATGCCGCAGTTCGCCGGAGCTTTCTCGCTCCTCCTCCGCTCTGCTCGATGTTTCAGCTTCACTGAAACAAGCCGACCAGGTCCTGAAATTCGATCCCAAGGGTTTAATGTCTTTCCAGCAGCAGGAGCAGCTTGTTCCGGCGAACTCTTCCTCCTTCTCTCTGCAACTGCACCCTCGCGATGCTCTTCGCAATGCCGGACACAAGGACTACAAGAGCCTCGTCCTCTCACGCCTCGACCGCGACTCCTCTCGAGTTAAATCGCTCAACGACAGGCTTCAATTTGCCCTCAGTGACCTGAAGAGGTCGGACCTTCAGCCGTTGAAGACGGAGATTCTGCCGGAGGATCTGAGCACTCCGATTGTCTCCGGCTCCAGTCAGGGCAGCGGCGAGTATTTCTCCCGCGTCGGCGTCGGACAACCGGCCAAGCCATTCTACATGGTTCTCGATACCGGCAGCGATATCAATTGGCTTCAATGTCAGCCTTGTACGGATTGCTACCAGCAATCCGACCCGATTTTTGACCCGAGAACTTCCTCCTCTTTTGCTTCCCTTCCCTGCGAATCTCAGCAATGCCAAGCTCTAGAAATGTCTGGTTGTCGTGCTGCCAAGTGTCTTTACCAGGTCTCGTATGGCGATGGCTCCTTCACCGTCGGCGAGTTCGTCACTGAAACGCTGTCGTTCGGCAACTCCGGGACGATTAACAACGTCGCTCTCGGGTGCGGCCACGACAACGAGGGACTGTTCGTCGGATCCGCTGGATTGCTCGGACTCGGCGGCGGATCACTATCCCTCACTTCTCAGATGAAAGCGTCGTCGTTTTCGTACTGTCTTGTGGACCGCGACTCTGGTTCGTCTTCGAGCCTGGATTTCAATTCCGGCGCACCCAGTGACTCAGTGACTGCTCCATTGCTCAGAAGCGGAAGAGTCGATACTTTCTACTACGTCGGGCTGACCGGACTGAGCGTCGGCGGGCAGTTGCTGTCGATTCCACCGTCTCTGTTCCAAATGGACGATTCCGGGTACGGCGGAATCATCGTGGACTCTGGAACCGCCATAACTCGGCTGCAGACTCAGGCGTACAACTCCCTCCGCGATGCCTTCGTGAGGCTGACGCCGTACCTGAGGAAGACGAACGGGTTCGCACTATTCGACACGTGTTACGATCTGTCGTCCCAGTCGAGAGTAACGATTCCGACGGTGTCGTTCCACTTCTCCGGCGGGAAGTCCCTGATGCTGCCGCCGAAGAACTACCTGATCCCGGTGGACTCAGCCGGAACTTTCTGCTTTGCGTTCGCCCCAACGACGTCGTCTCTGTCGATCATAGGGAACGTTCAGCAGCAGGGGACACGTGTCAACTTCGATTTGGCAAACTCGCTCGTAGGGTTTTCACCCAATAAATGT

Protein sequence

VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFSPNKC

Homology

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BLAST of MC03g1216 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 2.0e-168
Identity = 305/494 (61.74%), Postives = 380/494 (76.92%), Query Frame = 0


Query: 2   LPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDP-KGLMSFQQQE 61
           L +  +   SLFL  T   S    +   + +LDV +SL+Q   +L  DP +  ++  + E
Sbjct: 7   LSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 66

Query: 62  QL---VPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSD 121
            L   V  NSSS  SL+LH RD    + HKDYKSL LSRL+RDSSRV  +  +++FA+  
Sbjct: 67  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 126

Query: 122 LKRSDLQPL---KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 181
           + RSDL+P+    T    EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGSD+N
Sbjct: 127 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVN 186

Query: 182 WLQCQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSF 241
           W+QC+PC DCYQQSDP+F+P +SS++ SL C + QC  LE S CR+ KCLYQVSYGDGSF
Sbjct: 187 WIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSF 246

Query: 242 TVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSY 301
           TVGE  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFSY
Sbjct: 247 TVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSY 306

Query: 302 CLVDRDSGSSSSLDFNS-GAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLF 361
           CLVDRDSG SSSLDFNS        TAPLLR+ ++DTFYYVGL+G SVGG+ + +P ++F
Sbjct: 307 CLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIF 366

Query: 362 QMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRK-TNGFALFDTCYDLSSQS 421
            +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  L+K ++  +LFDTCYD SS S
Sbjct: 367 DVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS 426

Query: 422 RVTIPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNF 481
            V +PTV+FHF+GGKSL LP KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR+ +
Sbjct: 427 TVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITY 486

Query: 482 DLANSLVGFSPNKC 486
           DL+ +++G S NKC
Sbjct: 487 DLSKNVIGLSGNKC 500

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BLAST of MC03g1216 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.5e-112
Identity = 247/491 (50.31%), Postives = 311/491 (63.34%), Query Frame = 0


Query: 4   VFTIFIFSLFLP-FTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQL 63
           +F++  F L LP F+S  S   L  +S +L   S    Q D     D + L+  + +   
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDS----DSESLLESEFESGS 68

Query: 64  VPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDL 123
              +SSS +L L   DAL  + +K    L  SRL RDS RVKS+      A     R+  
Sbjct: 69  DSESSSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI---ATLAAQIPGRNVT 128

Query: 124 QPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTD 183
              +    P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  
Sbjct: 129 HAPR----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR 188

Query: 184 CYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGC--RAAKCLYQVSYGDGSFTVGEFVT 243
           CY QSDPIFDPR S ++A++PC S  C+ L+ +GC  R   CLYQVSYGDGSFTVG+F T
Sbjct: 189 CYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFST 248

Query: 244 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL---TSQMKASSFSYCLVD 303
           ETL+F     +  VALGCGHDNEGLFVG+AGLLGLG G LS    T       FSYCLVD
Sbjct: 249 ETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVD 308

Query: 304 RDSGSS-SSLDFNSGAPSDSVT-APLLRSGRVDTFYYVGLTGLSVGG-QLLSIPPSLFQM 363
           R + S  SS+ F + A S      PLL + ++DTFYYVGL G+SVGG ++  +  SLF++
Sbjct: 309 RSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKL 368

Query: 364 DDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVT 423
           D  G GG+I+DSGT++TRL   AY ++RDAF      L++   F+LFDTC+DLS+ + V 
Sbjct: 369 DQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVK 428

Query: 424 IPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLA 483
           +PTV  HF G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA
Sbjct: 429 VPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLA 484

Query: 484 NSLVGFSPNKC 486
           +S VGF+P  C
Sbjct: 489 SSRVGFAPGGC 484

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BLAST of MC03g1216 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 9.0e-108
Identity = 197/424 (46.46%), Postives = 278/424 (65.57%), Query Frame = 0


Query: 66  NSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDLQPL 125
           +SS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+   +  +  SD    
Sbjct: 55  SSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD---- 114

Query: 126 KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 185
            +     D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+
Sbjct: 115 -SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 174

Query: 186 QSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSF 245
           QSDP+FDP  S S+  + C S  C  +E SGC +  C Y+V YGDGS+T G    ETL+F
Sbjct: 175 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 234

Query: 246 GNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRDSGS 305
             +  + NVA+GCGH N G+F+G+AGLLG+GGGS+S   Q+      +F YCLV R + S
Sbjct: 235 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 294

Query: 306 SSSLDFNSGA-PSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYGG 365
           + SL F   A P  +   PL+R+ R  +FYYVGL GL VGG  + +P  +F + ++G GG
Sbjct: 295 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 354

Query: 366 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSFH 425
           +++D+GTA+TRL T AY + RD F   T  L + +G ++FDTCYDLS    V +PTVSF+
Sbjct: 355 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 414

Query: 426 FSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFS 485
           F+ G  L LP +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  VGF 
Sbjct: 415 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

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BLAST of MC03g1216 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 3.8e-74
Identity = 156/347 (44.96%), Postives = 207/347 (59.65%), Query Frame = 0


Query: 145 GSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQQSDPIFDPRTSSSFASLPC 204
           G GEY   + +G PA+PF  ++DTGSD+ W QCQPCT C+ QS PIF+P+ SSSF++LPC
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 150

Query: 205 ESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSFGNSGTINNVALGCGHDNEG 264
            SQ CQAL    C    C Y   YGDGS T G   TETL+FG S +I N+  GCG +N+G
Sbjct: 151 SSQLCQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFG-SVSIPNITFGCGENNQG 210

Query: 265 LFVGS-AGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSSSSLDFNSGAPSDSVTAP--- 324
              G+ AGL+G+G G LSL SQ+  + FSYC+    S + S+L   S A S +  +P   
Sbjct: 211 FGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTT 270

Query: 325 LLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMD-DSGYGGIIVDSGTAITRLQTQAYN 384
           L++S ++ TFYY+ L GLSVG   L I PS F ++ ++G GGII+DSGT +T     AY 
Sbjct: 271 LIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQ 330

Query: 385 SLRDAFVRLTPYLRKTNGFALFDTCYDL-SSQSRVTIPTVSFHFSGGKSLMLPPKNYLIP 444
           S+R  F+            + FD C+   S  S + IPT   HF GG  L LP +NY I 
Sbjct: 331 SVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELPSENYFIS 390

Query: 445 VDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFSPNKC 486
             S G  C A   ++  +SI GN+QQQ   V +D  NS+V F+  +C
Sbjct: 391 -PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of MC03g1216 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.6e-72
Identity = 172/445 (38.65%), Postives = 237/445 (53.26%), Query Frame = 0


Query: 62  LVPANSSSFSLQLH-----PRDALR------NAGHKDYK-SLVLSRLDRDSSRVKSLNDR 121
           + P +S+S    LH     P+  LR      ++G    K  L+   + R   R++S+N  
Sbjct: 19  VAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKRAIKRGERRMRSINAM 78

Query: 122 LQFALSDLKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTG 181
           LQ +                    + TP+ +    G GEY   V +G P   F  ++DTG
Sbjct: 79  LQSS------------------SGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTG 138

Query: 182 SDINWLQCQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYG 241
           SD+ W QC+PCT C+ Q  PIF+P+ SSSF++LPCESQ CQ L    C   +C Y   YG
Sbjct: 139 SDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYG 198

Query: 242 DGSFTVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGS-AGLLGLGGGSLSLTSQMKA 301
           DGS T G   TET +F  S ++ N+A GCG DN+G   G+ AGL+G+G G LSL SQ+  
Sbjct: 199 DGSTTQGYMATETFTFETS-SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV 258

Query: 302 SSFSYCLVDRDSGSSSSLDFN---SGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLL 361
             FSYC+    S S S+L      SG P  S +  L+ S    T+YY+ L G++VGG  L
Sbjct: 259 GQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNL 318

Query: 362 SIPPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAF---VRLTPYLRKTNGFALFD 421
            IP S FQ+ D G GG+I+DSGT +T L   AYN++  AF   + L      ++G +   
Sbjct: 319 GIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLS--- 378

Query: 422 TCYDLSSQ-SRVTIPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTS-SLSIIG 481
           TC+   S  S V +P +S  F GG  L L  +N LI   + G  C A   ++   +SI G
Sbjct: 379 TCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILIS-PAEGVICLAMGSSSQLGISIFG 435

Query: 482 NVQQQGTRVNFDLANSLVGFSPNKC 486
           N+QQQ T+V +DL N  V F P +C
Sbjct: 439 NIQQQETQVLYDLQNLAVSFVPTQC 435

[Close]

BLAST of MC03g1216 vs. NCBI nr
Match: XP_022137431.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Momordica charantia])

HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0


Query: 1   VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQE 60
           VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQE
Sbjct: 2   VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQE 61

Query: 61  QLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 120
           QLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS
Sbjct: 62  QLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 121

Query: 121 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 180
           DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC
Sbjct: 122 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 181

Query: 181 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 240
           TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT
Sbjct: 182 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 241

Query: 241 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 300
           ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS
Sbjct: 242 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 301

Query: 301 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 360
           GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG
Sbjct: 302 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 361

Query: 361 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 420
           GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF
Sbjct: 362 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 421

Query: 421 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 480
           HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF
Sbjct: 422 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 481

Query: 481 SPNKC 485
           SPNKC
Sbjct: 482 SPNKC 486

[Close]

BLAST of MC03g1216 vs. NCBI nr
Match: KAG6584369.1 (Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019953.1 Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854 bits (2207), Expect = 1.64e-310
Identity = 436/488 (89.34%), Postives = 460/488 (94.26%), Query Frame = 0


Query: 5   FTIFIFSLFLPFTSCRSSPELSRSSS------ALLDVSASLKQADQVLKFDPKGLMSFQQ 64
           F IF FSL LPFT CR+SPELS SSS      A LDVSASLKQA+QVLKFDPK L+SFQQ
Sbjct: 12  FAIFFFSL-LPFTLCRTSPELSHSSSSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQ 71

Query: 65  QEQLVPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDL 124
           Q+++VP NSSS FSLQLH RDAL+NAGHKDYKSLVLSRLDRDSSRVKSLNDRL+F+LSDL
Sbjct: 72  QDEVVPVNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDL 131

Query: 125 KRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 184
           KRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDTGSDINWLQC
Sbjct: 132 KRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQC 191

Query: 185 QPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGE 244
           QPCTDCYQQ+DPIFDPRTSSSFASLPCES QCQALE SGCRAAKCLYQV+YGDGSFTVGE
Sbjct: 192 QPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTVGE 251

Query: 245 FVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 304
           FVTE+LSFGNSGTI+NVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVD
Sbjct: 252 FVTESLSFGNSGTISNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 311

Query: 305 RDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDS 364
           RDS SSS+L+FNS APSDSV APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD+
Sbjct: 312 RDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDA 371

Query: 365 GYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPT 424
           GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNGFALFDTCYDLSSQSRVTIPT
Sbjct: 372 GYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIPT 431

Query: 425 VSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSL 484
           +SF F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANSL
Sbjct: 432 LSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSL 491

[Close]

BLAST of MC03g1216 vs. NCBI nr
Match: XP_022924034.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata])

HSP 1 Score: 853 bits (2203), Expect = 5.72e-310
Identity = 436/489 (89.16%), Postives = 463/489 (94.68%), Query Frame = 0


Query: 2   LPVFTI--FIFSLFLPFTSCRSSPELSRSSS--ALLDVSASLKQADQVLKFDPKGLMSFQ 61
           LP+F+   F FSL LPFT CR+SPELS SSS  A LDVSASLKQA+QVLKFDPK L+SFQ
Sbjct: 7   LPLFSFASFFFSL-LPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKALLSFQ 66

Query: 62  QQEQLVPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSD 121
           QQ+++VP NSSS FSLQLH RDAL+NAGH+DYKSLVLSRLDRDSSRVKSLNDRL+F+LSD
Sbjct: 67  QQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSD 126

Query: 122 LKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 181
           LKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDTGSDINWLQ
Sbjct: 127 LKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQ 186

Query: 182 CQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVG 241
           CQPCTDCYQQ+DPIFDPRTSSSFASLPCES QCQALE SGCRAAKCLYQV+YGDGSFTVG
Sbjct: 187 CQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTVG 246

Query: 242 EFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV 301
           EFVTE+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 247 EFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV 306

Query: 302 DRDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDD 361
           DRDS SSS+L+FNS APSDSV APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD
Sbjct: 307 DRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDD 366

Query: 362 SGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIP 421
           +GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNGFALFDTCYDLSSQSRVTIP
Sbjct: 367 AGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIP 426

Query: 422 TVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 481
           T+SF F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANS
Sbjct: 427 TLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANS 486

Query: 482 LVGFSPNKC 485
           LVGFSPNKC
Sbjct: 487 LVGFSPNKC 494

[Close]

BLAST of MC03g1216 vs. NCBI nr
Match: XP_023519500.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 853 bits (2203), Expect = 5.94e-310
Identity = 434/485 (89.48%), Postives = 459/485 (94.64%), Query Frame = 0


Query: 5   FTIFIFSLFLPFTSCRSSPELSRSSS---ALLDVSASLKQADQVLKFDPKGLMSFQQQEQ 64
           F IF FSL LPFT CR+SPELS SSS   A LDVSASLKQA+QVLKFDPK L+SFQQQ++
Sbjct: 12  FAIFFFSL-LPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQDE 71

Query: 65  LVPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 124
           +VP NSSS FSLQLH RDAL+NAGHKDYKSLVLSRLDRDSSRVKSLNDRL+F+LSDLKRS
Sbjct: 72  VVPGNSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRS 131

Query: 125 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 184
           DLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDTGSDINWLQCQPC
Sbjct: 132 DLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQCQPC 191

Query: 185 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 244
           TDCYQQ+DPIFDPRTSSSFASLPCES QCQALE SGCRAAKCLYQV+YGDGSFTVGEFVT
Sbjct: 192 TDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVT 251

Query: 245 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 304
           E+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS
Sbjct: 252 ESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 311

Query: 305 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 364
            SSS+L+FNS APSDSV APLL+SGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD+GYG
Sbjct: 312 SSSSALEFNSAAPSDSVNAPLLKSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYG 371

Query: 365 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 424
           GIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNGFALFDTCYDLSSQSRVTIPT+SF
Sbjct: 372 GIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIPTLSF 431

Query: 425 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 484
            F+GG SL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANSLVGF
Sbjct: 432 QFAGGNSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLVGF 491

[Close]

BLAST of MC03g1216 vs. NCBI nr
Match: XP_023000890.1 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima])

HSP 1 Score: 848 bits (2190), Expect = 5.67e-308
Identity = 433/485 (89.28%), Postives = 457/485 (94.23%), Query Frame = 0


Query: 5   FTIFIFSLFLPFTSCRSSPELSRSSS---ALLDVSASLKQADQVLKFDPKGLMSFQQQEQ 64
           F IF FSL LPFT CR+SPELS SSS   A LDVSASLKQA+QVLKFDPK L+SFQQQ +
Sbjct: 12  FAIFFFSL-LPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQNE 71

Query: 65  LVPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 124
           +VPANSSS FSLQLH RDAL+NAGHKDYKSLVLSRLDRDSSRVKSLNDRL+F+LSDLKRS
Sbjct: 72  VVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRS 131

Query: 125 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 184
           DLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDTGSDINWLQCQPC
Sbjct: 132 DLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPC 191

Query: 185 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 244
           TDCYQQ+DPIFDPRTSSSFASL CES QCQALE SGCRAAKCLYQV+YGDGSFTVGEFVT
Sbjct: 192 TDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVT 251

Query: 245 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 304
           E+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASSFSYCLVDRDS
Sbjct: 252 ESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSFSYCLVDRDS 311

Query: 305 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 364
            SSS+L+FNS APSD+V APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD+GYG
Sbjct: 312 SSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYG 371

Query: 365 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 424
           GIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNG ALFDTCYDLSSQSRVTIPT+SF
Sbjct: 372 GIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSF 431

Query: 425 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 484
            F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANSL+GF
Sbjct: 432 QFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGF 491

[Close]

BLAST of MC03g1216 vs. ExPASy TrEMBL
Match: A0A6J1C6K9 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN=LOC111008878 PE=3 SV=1)

HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 485/485 (100.00%), Postives = 485/485 (100.00%), Query Frame = 0


Query: 1   VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQE 60
           VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQE
Sbjct: 2   VLPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQE 61

Query: 61  QLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 120
           QLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS
Sbjct: 62  QLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 121

Query: 121 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 180
           DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC
Sbjct: 122 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 181

Query: 181 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 240
           TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT
Sbjct: 182 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 241

Query: 241 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 300
           ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS
Sbjct: 242 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 301

Query: 301 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 360
           GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG
Sbjct: 302 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 361

Query: 361 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 420
           GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF
Sbjct: 362 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 421

Query: 421 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 480
           HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF
Sbjct: 422 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 481

Query: 481 SPNKC 485
           SPNKC
Sbjct: 482 SPNKC 486

[Close]

BLAST of MC03g1216 vs. ExPASy TrEMBL
Match: A0A6J1E815 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=LOC111431581 PE=3 SV=1)

HSP 1 Score: 853 bits (2203), Expect = 2.77e-310
Identity = 436/489 (89.16%), Postives = 463/489 (94.68%), Query Frame = 0


Query: 2   LPVFTI--FIFSLFLPFTSCRSSPELSRSSS--ALLDVSASLKQADQVLKFDPKGLMSFQ 61
           LP+F+   F FSL LPFT CR+SPELS SSS  A LDVSASLKQA+QVLKFDPK L+SFQ
Sbjct: 7   LPLFSFASFFFSL-LPFTLCRTSPELSHSSSSSAFLDVSASLKQANQVLKFDPKALLSFQ 66

Query: 62  QQEQLVPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSD 121
           QQ+++VP NSSS FSLQLH RDAL+NAGH+DYKSLVLSRLDRDSSRVKSLNDRL+F+LSD
Sbjct: 67  QQDEVVPVNSSSSFSLQLHSRDALQNAGHQDYKSLVLSRLDRDSSRVKSLNDRLEFSLSD 126

Query: 122 LKRSDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQ 181
           LKRSDLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVG+PAKPFYMVLDTGSDINWLQ
Sbjct: 127 LKRSDLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGRPAKPFYMVLDTGSDINWLQ 186

Query: 182 CQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVG 241
           CQPCTDCYQQ+DPIFDPRTSSSFASLPCES QCQALE SGCRAAKCLYQV+YGDGSFTVG
Sbjct: 187 CQPCTDCYQQTDPIFDPRTSSSFASLPCESPQCQALETSGCRAAKCLYQVAYGDGSFTVG 246

Query: 242 EFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV 301
           EFVTE+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV
Sbjct: 247 EFVTESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLV 306

Query: 302 DRDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDD 361
           DRDS SSS+L+FNS APSDSV APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD
Sbjct: 307 DRDSSSSSALEFNSAAPSDSVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDD 366

Query: 362 SGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIP 421
           +GYGGIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNGFALFDTCYDLSSQSRVTIP
Sbjct: 367 AGYGGIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGFALFDTCYDLSSQSRVTIP 426

Query: 422 TVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANS 481
           T+SF F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANS
Sbjct: 427 TLSFQFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANS 486

Query: 482 LVGFSPNKC 485
           LVGFSPNKC
Sbjct: 487 LVGFSPNKC 494

[Close]

BLAST of MC03g1216 vs. ExPASy TrEMBL
Match: A0A6J1KJJ8 (protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LOC111495193 PE=3 SV=1)

HSP 1 Score: 848 bits (2190), Expect = 2.75e-308
Identity = 433/485 (89.28%), Postives = 457/485 (94.23%), Query Frame = 0


Query: 5   FTIFIFSLFLPFTSCRSSPELSRSSS---ALLDVSASLKQADQVLKFDPKGLMSFQQQEQ 64
           F IF FSL LPFT CR+SPELS SSS   A LDVSASLKQA+QVLKFDPK L+SFQQQ +
Sbjct: 12  FAIFFFSL-LPFTLCRTSPELSHSSSSSSAFLDVSASLKQANQVLKFDPKALLSFQQQNE 71

Query: 65  LVPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRS 124
           +VPANSSS FSLQLH RDAL+NAGHKDYKSLVLSRLDRDSSRVKSLNDRL+F+LSDLKRS
Sbjct: 72  VVPANSSSSFSLQLHSRDALQNAGHKDYKSLVLSRLDRDSSRVKSLNDRLEFSLSDLKRS 131

Query: 125 DLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPC 184
           DLQPL+TEILPEDLSTPIVSG+SQGSGEYFSR+GVGQPAKPFYMVLDTGSDINWLQCQPC
Sbjct: 132 DLQPLQTEILPEDLSTPIVSGTSQGSGEYFSRIGVGQPAKPFYMVLDTGSDINWLQCQPC 191

Query: 185 TDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVT 244
           TDCYQQ+DPIFDPRTSSSFASL CES QCQALE SGCRAAKCLYQV+YGDGSFTVGEFVT
Sbjct: 192 TDCYQQTDPIFDPRTSSSFASLACESPQCQALETSGCRAAKCLYQVAYGDGSFTVGEFVT 251

Query: 245 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS 304
           E+LSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL SQMKASSFSYCLVDRDS
Sbjct: 252 ESLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLISQMKASSFSYCLVDRDS 311

Query: 305 GSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYG 364
            SSS+L+FNS APSD+V APLLRSGRVDTFYYVGL GLSVGGQLLSIPPSLFQMDD+GYG
Sbjct: 312 SSSSALEFNSAAPSDTVNAPLLRSGRVDTFYYVGLVGLSVGGQLLSIPPSLFQMDDAGYG 371

Query: 365 GIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSF 424
           GIIVDSGTAITRLQTQAYN+LRDAFV  TPYLR+TNG ALFDTCYDLSSQSRVTIPT+SF
Sbjct: 372 GIIVDSGTAITRLQTQAYNTLRDAFVSRTPYLRRTNGVALFDTCYDLSSQSRVTIPTLSF 431

Query: 425 HFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGF 484
            F+GGKSL LPPKNYLIPVDS GTFC AFAPTTSSLSIIGNVQQQGTRV+FDLANSL+GF
Sbjct: 432 QFAGGKSLQLPPKNYLIPVDSVGTFCLAFAPTTSSLSIIGNVQQQGTRVHFDLANSLLGF 491

[Close]

BLAST of MC03g1216 vs. ExPASy TrEMBL
Match: A0A5D3BI72 (Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001860 PE=3 SV=1)

HSP 1 Score: 830 bits (2143), Expect = 3.16e-301
Identity = 421/480 (87.71%), Postives = 450/480 (93.75%), Query Frame = 0


Query: 7   IFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQLVPAN 66
           +F FSLF PF   RSS  LS  SSA LDVSASL+QA+QVLKFDP   +SFQQQ +LVP+N
Sbjct: 11  LFFFSLF-PFALSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQQVRLVPSN 70

Query: 67  SS-SFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDLQPL 126
           SS SFSLQLHPRD+L NAGHKDYKSLVLSRL RDSSRVKS+ DRL+FALS+LKRSDL+PL
Sbjct: 71  SSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELKRSDLEPL 130

Query: 127 KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 186
           KTEILPEDLSTPIVSG+SQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ
Sbjct: 131 KTEILPEDLSTPIVSGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 190

Query: 187 QSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSF 246
           Q+DPIFDPR+SSSFASLPCESQQCQALE SGCRA+KCLYQVSYGDGSFTVGEFVTETL+F
Sbjct: 191 QTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFVTETLTF 250

Query: 247 GNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSSSS 306
           GNSG I NVA+GCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS SSS 
Sbjct: 251 GNSGMIKNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSSSSSD 310

Query: 307 LDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYGGIIVD 366
           L+FNS AP+DSV APLL+SGRVDTFYYVGLTG+SVGGQLLSIPP+LFQMDDSGYGGIIVD
Sbjct: 311 LEFNSAAPTDSVNAPLLKSGRVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDSGYGGIIVD 370

Query: 367 SGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSFHFSGG 426
           SGTAITRLQTQAYN+LRDAFV  TPYL+KTNGFALFDTCYDLSSQSRVTIPTVSF F+GG
Sbjct: 371 SGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFEFAGG 430

Query: 427 KSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFSPNKC 485
           KSL LPPKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTRV++DLANS+VGFSP+KC
Sbjct: 431 KSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSVVGFSPHKC 489

[Close]

BLAST of MC03g1216 vs. ExPASy TrEMBL
Match: A0A0A0LQD2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042220 PE=3 SV=1)

HSP 1 Score: 828 bits (2140), Expect = 9.75e-301
Identity = 420/480 (87.50%), Postives = 449/480 (93.54%), Query Frame = 0


Query: 7   IFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQLVPAN 66
           +F  SLF PFT  RSS  LS  SSA LDVSASL+QA+QVLKFDP   +SFQQQ  LVP+N
Sbjct: 13  LFFLSLF-PFTLSRSSSHLSPHSSASLDVSASLQQANQVLKFDPTASISFQQQVHLVPSN 72

Query: 67  SS-SFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDLQPL 126
           SS SFSLQLHPRD+L NAGHKDYKSLVLSRL RDSSRVKS+ DRL+FALS+LKRSDL+PL
Sbjct: 73  SSFSFSLQLHPRDSLHNAGHKDYKSLVLSRLSRDSSRVKSIYDRLEFALSELKRSDLEPL 132

Query: 127 KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 186
           KTEILPEDLSTPI+SG+SQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ
Sbjct: 133 KTEILPEDLSTPIISGTSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 192

Query: 187 QSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSF 246
           Q+DPIFDPR+SSSFASLPCESQQCQALE SGCRA+KCLYQVSYGDGSFTVGEFV ETL+F
Sbjct: 193 QTDPIFDPRSSSSFASLPCESQQCQALETSGCRASKCLYQVSYGDGSFTVGEFVIETLTF 252

Query: 247 GNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSGSSSS 306
           GNSG INNVA+GCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDS SSS 
Sbjct: 253 GNSGMINNVAVGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVDRDSSSSSD 312

Query: 307 LDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYGGIIVD 366
           L+FNS APSDSV APLL+SG+VDTFYYVGLTG+SVGGQLLSIPP+LFQMDDSGYGGIIVD
Sbjct: 313 LEFNSAAPSDSVNAPLLKSGKVDTFYYVGLTGMSVGGQLLSIPPNLFQMDDSGYGGIIVD 372

Query: 367 SGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSFHFSGG 426
           SGTAITRLQTQAYN+LRDAFV  TPYL+KTNGFALFDTCYDLSSQSRVTIPTVSF F+GG
Sbjct: 373 SGTAITRLQTQAYNTLRDAFVSRTPYLKKTNGFALFDTCYDLSSQSRVTIPTVSFEFAGG 432

Query: 427 KSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFSPNKC 485
           KSL LPPKNYLIPVDS GTFCFAFAPTTSSLSIIGNVQQQGTRV++DLANS+VGFSP+KC
Sbjct: 433 KSLQLPPKNYLIPVDSVGTFCFAFAPTTSSLSIIGNVQQQGTRVHYDLANSVVGFSPHKC 491

[Close]

BLAST of MC03g1216 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 593.6 bits (1529), Expect = 1.5e-169
Identity = 305/494 (61.74%), Postives = 380/494 (76.92%), Query Frame = 0


Query: 2   LPVFTIFIFSLFLPFTSCRSSPELSRSSSALLDVSASLKQADQVLKFDP-KGLMSFQQQE 61
           L +  +   SLFL  T   S    +   + +LDV +SL+Q   +L  DP +  ++  + E
Sbjct: 7   LSLLAVVTLSLFLTTTDASSRSLSTPPKTNVLDVVSSLQQTQTILSLDPTRSSLTTTKPE 66

Query: 62  QL---VPANSSS-FSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSD 121
            L   V  NSSS  SL+LH RD    + HKDYKSL LSRL+RDSSRV  +  +++FA+  
Sbjct: 67  SLSDPVFFNSSSPLSLELHSRDTFVASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEG 126

Query: 122 LKRSDLQPL---KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDIN 181
           + RSDL+P+    T    EDL+TP+VSG+SQGSGEYFSR+GVG PAK  Y+VLDTGSD+N
Sbjct: 127 VDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVN 186

Query: 182 WLQCQPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSF 241
           W+QC+PC DCYQQSDP+F+P +SS++ SL C + QC  LE S CR+ KCLYQVSYGDGSF
Sbjct: 187 WIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSF 246

Query: 242 TVGEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSY 301
           TVGE  T+T++FGNSG INNVALGCGHDNEGLF G+AGLLGLGGG LS+T+QMKA+SFSY
Sbjct: 247 TVGELATDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQMKATSFSY 306

Query: 302 CLVDRDSGSSSSLDFNS-GAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLF 361
           CLVDRDSG SSSLDFNS        TAPLLR+ ++DTFYYVGL+G SVGG+ + +P ++F
Sbjct: 307 CLVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIF 366

Query: 362 QMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRK-TNGFALFDTCYDLSSQS 421
            +D SG GG+I+D GTA+TRLQTQAYNSLRDAF++LT  L+K ++  +LFDTCYD SS S
Sbjct: 367 DVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLS 426

Query: 422 RVTIPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNF 481
            V +PTV+FHF+GGKSL LP KNYLIPVD +GTFCFAFAPT+SSLSIIGNVQQQGTR+ +
Sbjct: 427 TVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITY 486

Query: 482 DLANSLVGFSPNKC 486
           DL+ +++G S NKC
Sbjct: 487 DLSKNVIGLSGNKC 500

[Close]

BLAST of MC03g1216 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 580.1 bits (1494), Expect = 1.7e-165
Identity = 291/488 (59.63%), Postives = 374/488 (76.64%), Query Frame = 0


Query: 3   PVFTIFIFSLFLPFTS---CRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQ 62
           P ++ F F  FL   S    R  PE S +++++L+V+ S+ +      F         QQ
Sbjct: 3   PNYSFFFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSF------RLNQQ 62

Query: 63  EQLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKR 122
           E+   + SSSFSLQLH R ++R   H DYKSL L+RL+RD++RVKSL  RL  A++++ +
Sbjct: 63  EEQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISK 122

Query: 123 SDLQPLKTEILPE--DLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQC 182
           +DL+P+ T    E  D+  P++SG++QGSGEYF+RVG+G+PA+  YMVLDTGSD+NWLQC
Sbjct: 123 ADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQC 182

Query: 183 QPCTDCYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGE 242
            PC DCY Q++PIF+P +SSS+  L C++ QC ALE+S CR A CLY+VSYGDGS+TVG+
Sbjct: 183 TPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGD 242

Query: 243 FVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMKASSFSYCLVD 302
           F TETL+ G S  + NVA+GCGH NEGLFVG+AGLLGLGGG L+L SQ+  +SFSYCLVD
Sbjct: 243 FATETLTIG-STLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVD 302

Query: 303 RDSGSSSSLDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDS 362
           RDS S+S++DF +    D+V APLLR+ ++DTFYY+GLTG+SVGG+LL IP S F+MD+S
Sbjct: 303 RDSDSASTVDFGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDES 362

Query: 363 GYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPT 422
           G GGII+DSGTA+TRLQT+ YNSLRD+FV+ T  L K  G A+FDTCY+LS+++ V +PT
Sbjct: 363 GSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVPT 422

Query: 423 VSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSL 482
           V+FHF GGK L LP KNY+IPVDS GTFC AFAPT SSL+IIGNVQQQGTRV FDLANSL
Sbjct: 423 VAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANSL 482

Query: 483 VGFSPNKC 486
           +GFS NKC
Sbjct: 483 IGFSSNKC 483

[Close]

BLAST of MC03g1216 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.5e-113
Identity = 247/491 (50.31%), Postives = 311/491 (63.34%), Query Frame = 0


Query: 4   VFTIFIFSLFLP-FTSCRSSPELSRSSSALLDVSASLKQADQVLKFDPKGLMSFQQQEQL 63
           +F++  F L LP F+S  S   L  +S +L   S    Q D     D + L+  + +   
Sbjct: 9   LFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSFQPDS----DSESLLESEFESGS 68

Query: 64  VPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDL 123
              +SSS +L L   DAL  + +K    L  SRL RDS RVKS+      A     R+  
Sbjct: 69  DSESSSSITLNLDHIDAL--SSNKTPDELFSSRLQRDSRRVKSI---ATLAAQIPGRNVT 128

Query: 124 QPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTD 183
              +    P   S+ +VSG SQGSGEYF+R+GVG PA+  YMVLDTGSDI WLQC PC  
Sbjct: 129 HAPR----PGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRR 188

Query: 184 CYQQSDPIFDPRTSSSFASLPCESQQCQALEMSGC--RAAKCLYQVSYGDGSFTVGEFVT 243
           CY QSDPIFDPR S ++A++PC S  C+ L+ +GC  R   CLYQVSYGDGSFTVG+F T
Sbjct: 189 CYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFST 248

Query: 244 ETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSL---TSQMKASSFSYCLVD 303
           ETL+F     +  VALGCGHDNEGLFVG+AGLLGLG G LS    T       FSYCLVD
Sbjct: 249 ETLTF-RRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVD 308

Query: 304 RDSGSS-SSLDFNSGAPSDSVT-APLLRSGRVDTFYYVGLTGLSVGG-QLLSIPPSLFQM 363
           R + S  SS+ F + A S      PLL + ++DTFYYVGL G+SVGG ++  +  SLF++
Sbjct: 309 RSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKL 368

Query: 364 DDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVT 423
           D  G GG+I+DSGT++TRL   AY ++RDAF      L++   F+LFDTC+DLS+ + V 
Sbjct: 369 DQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVK 428

Query: 424 IPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLA 483
           +PTV  HF G   + LP  NYLIPVD+ G FCFAFA T   LSIIGN+QQQG RV +DLA
Sbjct: 429 VPTVVLHFRGA-DVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLA 484

Query: 484 NSLVGFSPNKC 486
           +S VGF+P  C
Sbjct: 489 SSRVGFAPGGC 484

[Close]

BLAST of MC03g1216 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 393.3 bits (1009), Expect = 2.9e-109
Identity = 221/440 (50.23%), Postives = 285/440 (64.77%), Query Frame = 0


Query: 60  EQLVPANSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKR 119
           ++ +  +++S S+ L   DAL +        L   RL RDS RVKS+      +L+ +  
Sbjct: 51  DESLSESTTSLSVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKSIT-----SLAAVST 110

Query: 120 SDLQPLKTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQP 179
                 +T       S  ++SG SQGSGEYF R+GVG PA   YMVLDTGSD+ WLQC P
Sbjct: 111 GRNATKRTPRTAGGFSGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSP 170

Query: 180 CTDCYQQSDPIFDPRTSSSFASLPCESQQCQAL-EMSGC---RAAKCLYQVSYGDGSFTV 239
           C  CY Q+D IFDP+ S +FA++PC S+ C+ L + S C   R+  CLYQVSYGDGSFT 
Sbjct: 171 CKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTE 230

Query: 240 GEFVTETLSFGNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK---ASSFS 299
           G+F TETL+F +   +++V LGCGHDNEGLFVG+AGLLGLG G LS  SQ K      FS
Sbjct: 231 GDFSTETLTF-HGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFS 290

Query: 300 YCLVDRDSGSSSS------LDFNSGAPSDSVTAPLLRSGRVDTFYYVGLTGLSVGG-QLL 359
           YCLVDR S  SSS      +  N+  P  SV  PLL + ++DTFYY+ L G+SVGG ++ 
Sbjct: 291 YCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVP 350

Query: 360 SIPPSLFQMDDSGYGGIIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCY 419
            +  S F++D +G GG+I+DSGT++TRL   AY +LRDAF      L++   ++LFDTC+
Sbjct: 351 GVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCF 410

Query: 420 DLSSQSRVTIPTVSFHFSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQ 479
           DLS  + V +PTV FHF GG+ + LP  NYLIPV++ G FCFAFA T  SLSIIGN+QQQ
Sbjct: 411 DLSGMTTVKVPTVVFHFGGGE-VSLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQ 470

Query: 480 GTRVNFDLANSLVGFSPNKC 486
           G RV +DL  S VGF    C
Sbjct: 471 GFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of MC03g1216 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 392.1 bits (1006), Expect = 6.4e-109
Identity = 197/424 (46.46%), Postives = 278/424 (65.57%), Query Frame = 0


Query: 66  NSSSFSLQLHPRDALRNAGHKDYKSLVLSRLDRDSSRVKSLNDRLQFALSDLKRSDLQPL 125
           +SS ++L+L  RD   +  ++++   + +R+ RD+ RV ++  R+   +  +  SD    
Sbjct: 55  SSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKV--IPSSD---- 114

Query: 126 KTEILPEDLSTPIVSGSSQGSGEYFSRVGVGQPAKPFYMVLDTGSDINWLQCQPCTDCYQ 185
            +     D  + IVSG  QGSGEYF R+GVG P +  YMV+D+GSD+ W+QCQPC  CY+
Sbjct: 115 -SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYK 174

Query: 186 QSDPIFDPRTSSSFASLPCESQQCQALEMSGCRAAKCLYQVSYGDGSFTVGEFVTETLSF 245
           QSDP+FDP  S S+  + C S  C  +E SGC +  C Y+V YGDGS+T G    ETL+F
Sbjct: 175 QSDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTF 234

Query: 246 GNSGTINNVALGCGHDNEGLFVGSAGLLGLGGGSLSLTSQMK---ASSFSYCLVDRDSGS 305
             +  + NVA+GCGH N G+F+G+AGLLG+GGGS+S   Q+      +F YCLV R + S
Sbjct: 235 AKT-VVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS 294

Query: 306 SSSLDFNSGA-PSDSVTAPLLRSGRVDTFYYVGLTGLSVGGQLLSIPPSLFQMDDSGYGG 365
           + SL F   A P  +   PL+R+ R  +FYYVGL GL VGG  + +P  +F + ++G GG
Sbjct: 295 TGSLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGG 354

Query: 366 IIVDSGTAITRLQTQAYNSLRDAFVRLTPYLRKTNGFALFDTCYDLSSQSRVTIPTVSFH 425
           +++D+GTA+TRL T AY + RD F   T  L + +G ++FDTCYDLS    V +PTVSF+
Sbjct: 355 VVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFY 414

Query: 426 FSGGKSLMLPPKNYLIPVDSAGTFCFAFAPTTSSLSIIGNVQQQGTRVNFDLANSLVGFS 485
           F+ G  L LP +N+L+PVD +GT+CFAFA + + LSIIGN+QQ+G +V+FD AN  VGF 
Sbjct: 415 FTEGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFG 470

The following BLAST results are available for this feature:

BLAST of MC03g1216 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LS40	2.0e-168	61.74	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	3.5e-112	50.31	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	9.0e-108	46.46	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q766C3	3.8e-74	44.96	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.6e-72	38.65	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]

BLAST of MC03g1216 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137431.1	0.0	100.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Momordica charantia]	[more]
KAG6584369.1	1.64e-310	89.34	Protein ASPARTIC PROTEASE IN GUARD CELL 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022924034.1	5.72e-310	89.16	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita moschata]	[more]
XP_023519500.1	5.94e-310	89.48	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023000890.1	5.67e-308	89.28	protein ASPARTIC PROTEASE IN GUARD CELL 1-like [Cucurbita maxima]	[more]

BLAST of MC03g1216 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C6K9	0.0	100.00	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1E815	2.77e-310	89.16	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1KJJ8	2.75e-308	89.28	protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A5D3BI72	3.16e-301	87.71	Protein ASPARTIC PROTEASE IN GUARD CELL 1-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0LQD2	9.75e-301	87.50	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G04222...	[more]

BLAST of MC03g1216 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18490.1	1.5e-169	61.74	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	1.7e-165	59.63	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	2.5e-113	50.31	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	2.9e-109	50.23	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	6.4e-109	46.46	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 330..481 e-value: 3.9E-37 score: 127.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 309..485 e-value: 1.0E-51 score: 177.3
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 125..307 e-value: 1.7E-50 score: 173.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 143..485
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 149..306 e-value: 7.3E-48 score: 163.1
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 12..485
None	No IPR available	PANTHER	PTHR13683:SF274	PROTEIN ASPARTIC PROTEASE IN GUARD CELL 1	coord: 12..485
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 164..175
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 149..481 score: 48.268166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g1216.1	MC03g1216.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity