MC03g1114 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g1114
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin-protein ligase CIP8-like
Location	MC03: 17368881 .. 17369510 (-)
RNA-Seq Expression	MC03g1114
Synteny	MC03g1114

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGGGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAA

mRNA sequence

GCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGGGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAA

Coding sequence (CDS)

GCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGGGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAA

Protein sequence

AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME

Homology

[Close]

BLAST of MC03g1114 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.8e-11
Identity = 40/95 (42.11%), Postives = 55/95 (57.89%), Query Frame = 0


Query: 116 LVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIE--LGICGGDCAICLDEIECG 175
           L+  L + E SG+G   R  N P  AS+++IE + R EI         +CA+C +  E G
Sbjct: 169 LLEQLSQIEASGNGIG-RSGNPP--ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAG 228

Query: 176 AVG--MPCSHIYHRNCIVKWLQQNNLCPLCRFQMP 207
             G  MPC HI+H +CIV WL   N CP+CRF++P
Sbjct: 229 IEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of MC03g1114 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.6e-11
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 124 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 183
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 184 IYHRNCIVKWLQQNNLCPLCRFQMP 207
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of MC03g1114 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-10
Identity = 31/103 (30.10%), Postives = 61/103 (59.22%), Query Frame = 0


Query: 111 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGD---CAICL 170
           P + +L+  L E + +  G+ P        AS+++I+ +   ++   +   +   CA+C+
Sbjct: 169 PGLEQLIQQLAENDPNRYGTPP--------ASKSAIDALPTVKVTKDMLKSEMNQCAVCM 228

Query: 171 DEIECGA--VGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 209
           DE E G+    MPC H++H++C++ WL+ +N CP+CRF++P +
Sbjct: 229 DEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTD 263

[Close]

BLAST of MC03g1114 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-10
Identity = 32/81 (39.51%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 130 SDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSHIYHRNC 189
           SDP   NG   A + ++E +   +IE  +    C++CLD++E G  A  MPC H +H +C
Sbjct: 170 SDPS-RNGTPPAKKEAVEALPTVKIEEVV---SCSVCLDDLEVGSQAKQMPCEHKFHSSC 229

Query: 190 IVKWLQQNNLCPLCRFQMPLE 209
           I+ WL+ ++ CP+CRF++P E
Sbjct: 230 ILPWLELHSSCPVCRFELPSE 246

[Close]

BLAST of MC03g1114 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.9e-10
Identity = 40/97 (41.24%), Postives = 56/97 (57.73%), Query Frame = 0


Query: 116 LVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGD--CAICLD--EIE 175
           L+  L + E S +G   R  N P  AS+++IE + R EI     G +  CA+C +  E E
Sbjct: 153 LLEQLSQIEASATGIG-RSGNPP--ASKSAIESLPRVEISDCHIGSEANCAVCTEIFETE 212

Query: 176 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 209
             A  MPC H++H +CIV WL   N CP+CRF++P E
Sbjct: 213 TEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSE 246

[Close]

BLAST of MC03g1114 vs. NCBI nr
Match: XP_022137468.1 (E3 ubiquitin-protein ligase CIP8-like [Momordica charantia])

HSP 1 Score: 436 bits (1120), Expect = 3.48e-154
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVF 60
           AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVF
Sbjct: 4   AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVF 63

Query: 61  TQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPL 120
           TQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPL
Sbjct: 64  TQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPL 123

Query: 121 PEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCS 180
           PEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCS
Sbjct: 124 PEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCS 183

Query: 181 HIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
           HIYHRNCIVKWLQQNNLCPLCRFQMPLEME
Sbjct: 184 HIYHRNCIVKWLQQNNLCPLCRFQMPLEME 213

[Close]

BLAST of MC03g1114 vs. NCBI nr
Match: KAG6584266.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 289 bits (740), Expect = 3.48e-96
Identity = 151/217 (69.59%), Postives = 171/217 (78.80%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCS--SSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRD 60
           +GLHCEVWQPQ+++E+ CS  SSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R D
Sbjct: 11  SGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQRCD 70

Query: 61  VFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVL 120
           V TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVELVL
Sbjct: 71  VLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVL 130

Query: 121 PLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----ECG 180
           P    E+SG GSD  +  G  RASRASI+EMER E+  G+   DC ICLD+I     E  
Sbjct: 131 PEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMN-GVLS-DCVICLDDIVSVGSEID 190

Query: 181 AVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
           A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 191 ALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of MC03g1114 vs. NCBI nr
Match: XP_008437461.1 (PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa])

HSP 1 Score: 288 bits (737), Expect = 9.30e-96
Identity = 149/218 (68.35%), Postives = 170/218 (77.98%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60
           +GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRYKNFR+R 
Sbjct: 8   SGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFRQRC 67

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTIP+VVEL+
Sbjct: 68  DVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTIPMVVELM 127

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----EC 180
           LP    E SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI     E 
Sbjct: 128 LPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEID-GVLS-DCVICLDEIGSIGCEI 187

Query: 181 GAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
            AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 188 DAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of MC03g1114 vs. NCBI nr
Match: XP_023001523.1 (uncharacterized protein LOC111495638 [Cucurbita maxima])

HSP 1 Score: 288 bits (737), Expect = 9.95e-96
Identity = 149/217 (68.66%), Postives = 169/217 (77.88%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCS--SSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRD 60
           +GLHCEVWQPQ+++E+ CS  SSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R D
Sbjct: 11  SGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQRCD 70

Query: 61  VFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVL 120
           V TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVELVL
Sbjct: 71  VLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVL 130

Query: 121 PLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----ECG 180
           P    E+S  GSD  +  G  RASRASI+EMER E+   +   DC ICLD+I     E  
Sbjct: 131 PEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGAL--SDCVICLDDIVSVGSEID 190

Query: 181 AVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
           A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 191 ALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of MC03g1114 vs. NCBI nr
Match: KAG7019862.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 288 bits (736), Expect = 1.41e-95
Identity = 150/217 (69.12%), Postives = 170/217 (78.34%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCS--SSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRD 60
           +GLHCEVWQPQ+++E+ CS  SSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R D
Sbjct: 11  SGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQRCD 70

Query: 61  VFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVL 120
           V TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVELVL
Sbjct: 71  VLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVL 130

Query: 121 PLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----ECG 180
           P    E+SG GSD  +  G  RASR SI+EMER E+  G+   DC ICLD+I     E  
Sbjct: 131 PEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMN-GVLS-DCVICLDDIVSVGSEID 190

Query: 181 AVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
           A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 191 ALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of MC03g1114 vs. ExPASy TrEMBL
Match: A0A6J1CAF5 (E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC111008905 PE=4 SV=1)

HSP 1 Score: 436 bits (1120), Expect = 1.68e-154
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVF 60
           AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVF
Sbjct: 4   AGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVF 63

Query: 61  TQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPL 120
           TQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPL
Sbjct: 64  TQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPL 123

Query: 121 PEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCS 180
           PEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCS
Sbjct: 124 PEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCS 183

Query: 181 HIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
           HIYHRNCIVKWLQQNNLCPLCRFQMPLEME
Sbjct: 184 HIYHRNCIVKWLQQNNLCPLCRFQMPLEME 213

[Close]

BLAST of MC03g1114 vs. ExPASy TrEMBL
Match: A0A5D3BJ05 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003020 PE=4 SV=1)

HSP 1 Score: 288 bits (737), Expect = 4.50e-96
Identity = 149/218 (68.35%), Postives = 170/218 (77.98%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60
           +GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRYKNFR+R 
Sbjct: 8   SGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFRQRC 67

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTIP+VVEL+
Sbjct: 68  DVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTIPMVVELM 127

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----EC 180
           LP    E SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI     E 
Sbjct: 128 LPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEID-GVLS-DCVICLDEIGSIGCEI 187

Query: 181 GAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
            AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 188 DAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of MC03g1114 vs. ExPASy TrEMBL
Match: A0A1S3AU71 (uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=4 SV=1)

HSP 1 Score: 288 bits (737), Expect = 4.50e-96
Identity = 149/218 (68.35%), Postives = 170/218 (77.98%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60
           +GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRYKNFR+R 
Sbjct: 8   SGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFRQRC 67

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTIP+VVEL+
Sbjct: 68  DVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTIPMVVELM 127

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----EC 180
           LP    E SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI     E 
Sbjct: 128 LPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEID-GVLS-DCVICLDEIGSIGCEI 187

Query: 181 GAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
            AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 188 DAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of MC03g1114 vs. ExPASy TrEMBL
Match: A0A6J1KQS3 (uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638 PE=4 SV=1)

HSP 1 Score: 288 bits (737), Expect = 4.82e-96
Identity = 149/217 (68.66%), Postives = 169/217 (77.88%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCS--SSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRD 60
           +GLHCEVWQPQ+++E+ CS  SSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R D
Sbjct: 11  SGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQRCD 70

Query: 61  VFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVL 120
           V TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVELVL
Sbjct: 71  VLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVELVL 130

Query: 121 PLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----ECG 180
           P    E+S  GSD  +  G  RASRASI+EMER E+   +   DC ICLD+I     E  
Sbjct: 131 PEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGAL--SDCVICLDDIVSVGSEID 190

Query: 181 AVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
           A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 191 ALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of MC03g1114 vs. ExPASy TrEMBL
Match: A0A0A0LR28 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 PE=4 SV=1)

HSP 1 Score: 287 bits (735), Expect = 9.07e-96
Identity = 148/219 (67.58%), Postives = 169/219 (77.17%), Query Frame = 0


Query: 1   AGLHCEVWQPQEWAESDCSSSSS----EPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 60
           +GLHCEVWQPQ+++E  CSSSSS    +PPEFFID YVSSYA  G E IS+LRYKNFR+R
Sbjct: 7   SGLHCEVWQPQDFSEPSCSSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFRQR 66

Query: 61  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 120
            DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS RAR IA +P NALSRTIP+VVEL
Sbjct: 67  CDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISTRARGIATEPINALSRTIPMVVEL 126

Query: 121 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 180
           +LP    E+SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI     E
Sbjct: 127 ILPEDAMEDSGYGSEPHMGIGSGRASRASIQEMERIEID-GVLS-DCVICLDEIGSIGCE 186

Query: 181 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 210
              V MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 187 IDVVQMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of MC03g1114 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 69.3 bits (168), Expect = 4.1e-12
Identity = 40/95 (42.11%), Postives = 55/95 (57.89%), Query Frame = 0


Query: 116 LVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIE--LGICGGDCAICLDEIECG 175
           L+  L + E SG+G   R  N P  AS+++IE + R EI         +CA+C +  E G
Sbjct: 169 LLEQLSQIEASGNGIG-RSGNPP--ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAG 228

Query: 176 AVG--MPCSHIYHRNCIVKWLQQNNLCPLCRFQMP 207
             G  MPC HI+H +CIV WL   N CP+CRF++P
Sbjct: 229 IEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELP 260

[Close]

BLAST of MC03g1114 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-12
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 124 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 183
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 184 IYHRNCIVKWLQQNNLCPLCRFQMP 207
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of MC03g1114 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-12
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 124 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 183
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 184 IYHRNCIVKWLQQNNLCPLCRFQMP 207
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of MC03g1114 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 68.9 bits (167), Expect = 5.4e-12
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 124 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 183
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 184 IYHRNCIVKWLQQNNLCPLCRFQMP 207
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of MC03g1114 vs. TAIR 10
Match: AT3G10815.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 9.2e-12
Identity = 33/74 (44.59%), Postives = 44/74 (59.46%), Query Frame = 0


Query: 137 GPGRASRASIEEMERAEIELGICGGD--CAICLDEIECG--AVGMPCSHIYHRNCIVKWL 196
           GP  AS A+I  +++ +I     G D  C +C D+ E G  A  MPC HIYH  CI+ WL
Sbjct: 93  GPPPASLAAINSLQKIKIRQKHLGLDPYCPVCQDQFEIGSDARKMPCKHIYHSECILPWL 152

Query: 197 QQNNLCPLCRFQMP 207
            Q N CP+CR ++P
Sbjct: 153 VQRNTCPVCRKELP 166

The following BLAST results are available for this feature:

BLAST of MC03g1114 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SNB6	5.8e-11	42.11	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
O22197	7.6e-11	38.82	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q8LPN7	1.7e-10	30.10	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q6AVN2	1.7e-10	39.51	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q940T5	2.9e-10	41.24	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of MC03g1114 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137468.1	3.48e-154	100.00	E3 ubiquitin-protein ligase CIP8-like [Momordica charantia]	[more]
KAG6584266.1	3.48e-96	69.59	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_008437461.1	9.30e-96	68.35	PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ub...	[more]
XP_023001523.1	9.95e-96	68.66	uncharacterized protein LOC111495638 [Cucurbita maxima]	[more]
KAG7019862.1	1.41e-95	69.12	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of MC03g1114 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAF5	1.68e-154	100.00	E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A5D3BJ05	4.50e-96	68.35	E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3AU71	4.50e-96	68.35	uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=...	[more]
A0A6J1KQS3	4.82e-96	68.66	uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638...	[more]
A0A0A0LR28	9.07e-96	67.58	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 P...	[more]

BLAST of MC03g1114 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46620.1	4.1e-12	42.11	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G40830.2	5.4e-12	38.82	RING-H2 finger C1A	[more]
AT2G40830.1	5.4e-12	38.82	RING-H2 finger C1A	[more]
AT2G40830.3	5.4e-12	38.82	RING-H2 finger C1A	[more]
AT3G10815.1	9.2e-12	44.59	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 163..201 e-value: 1.4E-6 score: 37.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 162..202 e-value: 2.5E-11 score: 43.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 163..202 score: 12.54829
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 133..207 e-value: 1.8E-16 score: 61.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 123..143
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 4..206
None	No IPR available	PANTHER	PTHR15710:SF138	FINGER PROTEIN, PUTATIVE-RELATED	coord: 4..206
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 162..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g1114.1	MC03g1114.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity