MC03g1086 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g1086
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ef-hand calcium binding protein
Location	MC03: 17125365 .. 17130094 (+)
RNA-Seq Expression	MC03g1086
Synteny	MC03g1086

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAATTTTGCCACAAAGATGCTATATAAATATCCCCACAGAAAGAAGACTTTCAAAAAAATCACATTAGTACATCGGATCAGCAATAATATGTCGGGTTACCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGAGCCGGCGGTGGATACGGAGGCTACGGAGCTCCTCCGCCAACTGCCCAGCCCTACGCCTCTTCGGCGTACGGCGGACAACAACCACCGCCTCACTCTCAGCAGTCGTACGCTCCGGTGTCCGCCCCGTACGGCGCACCGTCGGCACCGTACGGATCATCTACCGGAGACAAACCTCCGAAGGACAAGCCTCATCAGTCCTACGGCGGGGCTGGTGGCGGTCATCAATCTTACGGTGGAGCCGGTGGCGGTCATGCTTATCCTCCACCTAATTCGTACGGAAACCCTTTCGCGTCGCTCTTGCCCTCGGCCTTCCCGCCTGGTACTGATCCGAGCATCGTCGCCTGCTTCCAAGTAGCCGATCAGGATGGGAGTGGCTTTATCGATGATAAGGAGTTGCAAGGTGTTCTGTCCTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCACCTCCTCATGTACCATTTCACCAACACGAACACCAGGAAGATCGGTAATCGCACTTTCTCTTTATCACTTTTTCGTTTGCCCTGGAAATACGAAATTTCACGTCGTTTTAGTTTCTTAAATCAACAACAGTAATGATAAGATCCGGAAATTTCTGCTACATGCAAGTCATTAGTTCCAACATAGACGATGAGACCGTATCACATCACTTCAGCAAATTTTAATTGAAAGATAAACTGACAAAGATAAATGTATGTTCATAGAATAGGTCATCGGTTCATTATCAAAATTGTCGAGGTGAATTTCATGTAATCGCGTTGAATTGTAATGTTTGTTTTTAGTAATTATGATTATTTTTATAAAATTACTATTAGTTGATTAGTTCATGGTGCTCATACCTGTGGGGTATTTACTAGAAGATGCCAAATACGTTCTATTTATATTTCTGTTAATCCTATACTAAGATACCTCGGCTCAATCTTATTGCCTGCGATTAGTTAATGTTATTTCATGCTCTATTTTACATTTGTCTGGTCTGGTGGTTAGGTTAAGCAGTTGGGTTAATTACTCAAAATTTATGTAGAAAATTTAGAACCTACCATTTGGACTTGGATTAGTTTTGGTGGTTGCACGACTTTCACTATATTCAAATTGAGCAGTTGAAACTGTAGACTTCTCAAAACTCTGAACCTTTTGGGTTATTTGAGTGTCTGTCATTATGCAAGTATTTTTTTAAATGATAAAATGGTGTGGTATTTTGAATCAGAGCATATGGTTATATTGTCAATTTTTTAATCTGTTCGAATCATCTTACTCTTTTATGTATTTAAACCTCGAGTGGGGTGGGGGGTGCTGCTATAGATTTGATATGTGCTGTCTTGTGAATTGATCTCTAAATCATGATTTTACCTTGTCTATTGACATTGCTTTTCTATTTCTTTTCCTGAAAAACAGGGCCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGTAAGCTGAATATTTGATATTATTTGAAGCAAAAATTATTTGTTGGTGTAATTCTGTGAGATTGAGTATCTATTGTATCTTTTTCAGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAAAATCGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCGGTGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGCGGTGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTATGTTTGCCTTCCTTACTGTTGATCTGATGTGGTACTCTTTTTCTGATGAGGTTTCTTGTGATATTTAAGTGTTAAGAATTCTTTCGATCACAGTTCTTATTGTTTTCTTTTTGCAAATTCAGGTGCTGTCTTACGGTCAAGGTGAGCTTCTTCTGATTTGATTTGCATGTCTTCTAATTAGACTGATTAATATGAATATCTTTTACATATCACTGTATACTGTTTAGAGGTGATGGCATCATCTTTGAATCCACAGGCTTAGTGTGCAATCTGTTTAACTATTTGAGCATATTTGCCTACTTGTCATTACATCTACTGCTTAACCTTGAACTTGATTTTATTCCAACTCCTGTTGGTGCATGTGAATACACCGAACACCTGTTACACGAACCTGCATTTAACAAGCCTGTGGATCTAGTAGTCGCTTGTCATCTAATTTCACTATGTAAAATTGTTCTTGTTTTTATCCTTTTTCCCTTTCCTGTAACATGAAATGTTTTTTCACACACTGGTATTTTTAACACCTTGTATGAATTGAACCCGAGTCCTCGGCATCACATCTGCGTTGATATTTACGGCTCTCATAATCGTTTCTGTACCATGGCGACAAAGAAAGAGCTAAAACTAAAATGGTTTTGTTGTAAAATTTTTTTTTTACAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCTGGTTCTGCAACCTTCAGTTACGAGGCTTTCATGCTGACTGTCCTGCCATTCCTTATCGCCTGAGGAGTTTATCGGCCTTCGATCCAGCACCTGTTCTCAATGTAAAGCTCGAGTTTCCTTTGGCTTTTCTCTTCAATTTCTGTTTGTGTTTGTAGAATCTGTTTATGTAGCTTTATCATTATCAATGCTGTATGTTTGGTTTTTGTATAAGTTAAATCTCCTAAAGTATTAAATGATAGTTCTGATTAATGTCCCAACTAAATCTGTGACCCTTCCCAGATTTCTGTGAGTAAGCCGTAGGTTCAATCGATTACCGGGCATCGTCATTCCATCCCCTGACCCGCCTTTTGTGATAAATTCTGTGGATGAACTGAGGAGTACGAAAATGGCTTTAATTGCAAGTTATCTGGGTTTATTAGTGATAGGTGGGAGAGAAGGGTGATAAATGAGTTCTGTTCTGTTTTACAAGCTGATATTACTTCCTCTATTGGTGAATGACACATTATTCTTTGACAATTTCTTCCATACTTTTTTCATATAATTTCGTTTTTAGTCTTCAATTATTAAAAATAATGCTTGTTTTCTCACAATTTCGTTTGTTTTTAGTTCTCTGTTGGTAAAAGTTAGGCTTGTTTTCCCACTATTTTTTGGTATGTTTTTCACATTTCTTTTGTATTTTTTTAGCAAAATTTTAGAACAAAAACAAGTATTTGGTATTTTTTTTTTTGTCCTTTTTCAAGAATTTGGCTTAGATTTTGAAAATGGTCCTAAAAGGGTTGGTAACAAGACAAAGGAATACATTGGTGAAACGGTGGACTTTAATAGTTTGTTTTTTCATTTAACAATTAAGTGAGCACATAAATATCTATTCTATCTTCGTGAGGGATTTGAAATGGTAATAGGTTATTTATTCACGAGTTGAGATCCCTATTCATAGATTAGATTGGTAATTACTTCATAGTTAATTTATCTTTAAATGGGTAATTACTTCATATATCTTTAAAATATTGGCTTTTTATATTCTAACTATTTAGAATCTATTAAAAGTTTTCGAGGATCTTCGACCCATCTTTTTAAAGTATAATATGAGAGTGAAGATTCGAAGATTCGAATCTACGATCTTTTGGTCTAGAATATATGTTTTAATTAGTTGAGCTAGGTTCAGATTTGAGGTGTAAAGTTGTTTAGTTTGGAGTTATGTGTTTGGAGTGTGAATTAAAGTTAGATTCTAATGATTTTTTCTTTTCGTCAATTATAAAATTGAGATTATAAGTTATATTATTTTATGAAAATTTTGAAATTTTATTTAAGGTAGCTATTTTGATTAGGATAAGAAAAATTACATCAAGCACAATAATTTGAAACGATAATTTACAACCAAGGTAGAATGGCCAATGAAGGCCAAATTCATAATTCTGGAAAGAAAAAATAGTTGCAGGATAGGAGGGGCCGATCATTTGCTATTTCCCAACAGATGCATGCAATACATTTTTCTGAAATGTGGTGGGACGGACAAGAAACTGTTGGTCTTCTCGACGTCATCGAACAAAATATGTATAAGTTGGACGCTATCGTCCACGTCCCAAGCCTGGTATTTGTTCAAGCTGGTCGATCACTTCTTTCCTTCGTGCCACGCTGCAATCTTCTCAAGCTGAGTCGTTTGCAAACTCATTTCTGTCCGAATCACCTCTATCATATCCGATGTTAACAGATCCGGTGTTAGAGCCAATGCAAGTATTAACTGACGTTGGGTGGTGCCAAAGATTGTCAATATGGTAATTAGTTCGTGGCTTTTCATGATAATTAATTGGACAATGATATGTTCCTAAAACACAAATATAGTCGCTTCATATCATATTAGACACAAACATGTCGTTTGGTTTTTAGCTTTATTGGCCGTTCACCCATGTACAATTAAACTAATCCTTATATACATTACGATCATTCCAAGAATAAATCCTATCAATAGGTGCATAATACTATGAACTCTTCCTCCATGAACAATTTTCATGGAATTCAACTCAATCGTAAATAACTGCTAAGCTATGATTGCTTTGACGTTGATTAGCCACTTATTGCCATCTTAAATTTAAATTGGCCGCAAAACTTACCTATGATTTGTGAATCCCTTACCTACCAGTAATAAATACCACATACAACTTCATTTTTCC

mRNA sequence

GTAATTTTGCCACAAAGATGCTATATAAATATCCCCACAGAAAGAAGACTTTCAAAAAAATCACATTAGTACATCGGATCAGCAATAATATGTCGGGTTACCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGAGCCGGCGGTGGATACGGAGGCTACGGAGCTCCTCCGCCAACTGCCCAGCCCTACGCCTCTTCGGCGTACGGCGGACAACAACCACCGCCTCACTCTCAGCAGTCGTACGCTCCGGTGTCCGCCCCGTACGGCGCACCGTCGGCACCGTACGGATCATCTACCGGAGACAAACCTCCGAAGGACAAGCCTCATCAGTCCTACGGCGGGGCTGGTGGCGGTCATCAATCTTACGGTGGAGCCGGTGGCGGTCATGCTTATCCTCCACCTAATTCGTACGGAAACCCTTTCGCGTCGCTCTTGCCCTCGGCCTTCCCGCCTGGTACTGATCCGAGCATCGTCGCCTGCTTCCAAGTAGCCGATCAGGATGGGAGTGGCTTTATCGATGATAAGGAGTTGCAAGGTGTTCTGTCCTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCACCTCCTCATGTACCATTTCACCAACACGAACACCAGGAAGATCGGGCCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAAAATCGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCGGTGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGCGGTGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTGCTGTCTTACGGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCTGGTTCTGCAACCTTCAGTTACGAGGCTTTCATGCTGACTGTCCTGCCATTCCTTATCGCCTGAGGAGTTTATCGGCCTTCGATCCAGCACCTGTTCTCAATTTGCAGGATAGGAGGGGCCGATCATTTGCTATTTCCCAACAGATGCATGCAATACATTTTTCTGAAATGTGGTGGGACGGACAAGAAACTGTTGGTCTTCTCGACGTCATCGAACAAAATATGTATAAGTTGGACGCTATCGTCCACGTCCCAAGCCTGGTATTTGTTCAAGCTGGTCGATCACTTCTTTCCTTCGTGCCACGCTGCAATCTTCTCAAGCTGAGTCGTTTGCAAACTCATTTCTGTCCGAATCACCTCTATCATATCCGATGTTAACAGATCCGGTGTTAGAGCCAATGCAAGTATTAACTGACGTTGGGTGGTGCCAAAGATTGTCAATATGGTAATTAGTTCGTGGCTTTTCATGATAATTAATTGGACAATGATATGTTCCTAAAACACAAATATAGTCGCTTCATATCATATTAGACACAAACATGTCGTTTGGTTTTTAGCTTTATTGGCCGTTCACCCATGTACAATTAAACTAATCCTTATATACATTACGATCATTCCAAGAATAAATCCTATCAATAGGTGCATAATACTATGAACTCTTCCTCCATGAACAATTTTCATGGAATTCAACTCAATCGTAAATAACTGCTAAGCTATGATTGCTTTGACGTTGATTAGCCACTTATTGCCATCTTAAATTTAAATTGGCCGCAAAACTTACCTATGATTTGTGAATCCCTTACCTACCAGTAATAAATACCACATACAACTTCATTTTTCC

Coding sequence (CDS)

ATGCTATATAAATATCCCCACAGAAAGAAGACTTTCAAAAAAATCACATTAGTACATCGGATCAGCAATAATATGTCGGGTTACCCAAATCAGCCTTCCGGTTACGGTTACGGCTACGGCGGCGCCGGAGCCGGCGGTGGATACGGAGGCTACGGAGCTCCTCCGCCAACTGCCCAGCCCTACGCCTCTTCGGCGTACGGCGGACAACAACCACCGCCTCACTCTCAGCAGTCGTACGCTCCGGTGTCCGCCCCGTACGGCGCACCGTCGGCACCGTACGGATCATCTACCGGAGACAAACCTCCGAAGGACAAGCCTCATCAGTCCTACGGCGGGGCTGGTGGCGGTCATCAATCTTACGGTGGAGCCGGTGGCGGTCATGCTTATCCTCCACCTAATTCGTACGGAAACCCTTTCGCGTCGCTCTTGCCCTCGGCCTTCCCGCCTGGTACTGATCCGAGCATCGTCGCCTGCTTCCAAGTAGCCGATCAGGATGGGAGTGGCTTTATCGATGATAAGGAGTTGCAAGGTGTTCTGTCCTCGTATAATCAGAAGTTTAGCATCAGAACTGTTCACCTCCTCATGTACCATTTCACCAACACGAACACCAGGAAGATCGGGCCCAAGGAGTTCATTGCTCTGTTTTATGGTCTTCAGAGCTGGAGGGGTATTTTTGAGAGATTCGATTCAGATAGAAGTGGAAAAATCGATTCCAATGAGTTGCGAGAAGCACTTTTGAGTTTGGGCTTTGCAGTCTCACCGGTGGTCTTGGACTTGCTGGTGTCTAAGTTTGACAAAAGCGGTGGGAAAAGCAAGGCCATTGAATATGATAATTTCATAGAGTGCTGTCTTACGGTCAAGGGACTGACTGAGAAATTCAAGGAAAAGGACACCACATATTCTGGTTCTGCAACCTTCAGTTACGAGGCTTTCATGCTGACTGTCCTGCCATTCCTTATCGCCTGA

Protein sequence

MLYKYPHRKKTFKKITLVHRISNNMSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSAPYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA

Homology

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BLAST of MC03g1086 vs. ExPASy Swiss-Prot
Match: Q9FYE4 (Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.2e-100
Identity = 220/366 (60.11%), Postives = 242/366 (66.12%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYGYGG            PPP   PY+S   GG  PP  S  + +P + 
Sbjct: 1   MSGYPPTSQGYGYGYGGG---------NQPPPPQPPYSS---GGNNPPYGSSTTSSPYAV 60

Query: 85  PYGA----------------------PSAPYGSSTGD--KPPKDKP-------------- 144
           PYGA                      PSAPY  S GD  KPPK+KP              
Sbjct: 61  PYGASKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSS 120

Query: 145 -HQSYG---------GAGGG-----------HQSYGGA---------GGGH-AYPPPNSY 204
            + SYG         G GGG           + SYGGA         GGG+  YPP  SY
Sbjct: 121 DYGSYGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASY 180

Query: 205 GNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLM 264
           G+PFASL+PS F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLM
Sbjct: 181 GSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLM 240

Query: 265 YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPV 322
           Y FTN+N  KIGPKEF ALFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSPV
Sbjct: 241 YLFTNSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPV 300

[Close]

BLAST of MC03g1086 vs. ExPASy Swiss-Prot
Match: Q8W4L0 (Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.6e-100
Identity = 213/340 (62.65%), Postives = 238/340 (70.00%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 85  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 144
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 145 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 204
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 205 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 264
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 265 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 322
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 300

[Close]

BLAST of MC03g1086 vs. ExPASy Swiss-Prot
Match: Q2QY10 (Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 5.8e-95
Identity = 201/309 (65.05%), Postives = 229/309 (74.11%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPT--AQPYASSAYGGQQPPPHSQQSYAPV 84
           M+GYP  P G GY YGGA      GGYGAPPP   + P  S+   G +PP   + S +  
Sbjct: 1   MAGYPPNP-GSGYPYGGA------GGYGAPPPPYGSSPAPSAPPYGAKPPKEGKTSSS-- 60

Query: 85  SAPYGAPSAPYGSSTGDKPPKDKPHQSYGGAGG--GHQSYGGAGGGHAYPPPNS------ 144
           SAPY      YG+     PP  +P+ S GG G     Q YG   G    PPP+S      
Sbjct: 61  SAPYYGGGGGYGA-----PPSTQPYGSGGGYGAPPSSQPYGAPYGA---PPPSSAPYGAP 120

Query: 145 --YGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVH 204
             YG+PFASL+PSAFPPGTDP++VACFQ AD+DGSG IDDKELQ  LS Y+Q FS+RTVH
Sbjct: 121 GGYGSPFASLVPSAFPPGTDPNVVACFQAADRDGSGMIDDKELQSALSGYSQSFSLRTVH 180

Query: 205 LLMYHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAV 264
           LLMY FTNTN RKIGPKEF ++FY LQ+WR IFERFD DRSGKID+ ELR+ALLSLG++V
Sbjct: 181 LLMYLFTNTNVRKIGPKEFTSVFYSLQNWRSIFERFDRDRSGKIDATELRDALLSLGYSV 240

Query: 265 SPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 322
           SP VLDLLVSKFDK+GGK+KAIEYDNFIECCLTVKGLTEKFKEKDT +SGSATF+YEAFM
Sbjct: 241 SPTVLDLLVSKFDKTGGKNKAIEYDNFIECCLTVKGLTEKFKEKDTAFSGSATFTYEAFM 292

[Close]

BLAST of MC03g1086 vs. ExPASy Swiss-Prot
Match: Q9ZQH1 (Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 PE=2 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 3.0e-35
Identity = 83/196 (42.35%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 127 HAYPPPNSYGNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLS-SYNQK 186
           + Y  P+       S     F P T P IV  F+ AD++ SGF+++ EL+  LS S    
Sbjct: 29  YTYAYPSYQPTQQFSSYSGMFSPETHPEIVRSFESADRNRSGFLEESELRQALSLSGYDG 88

Query: 187 FSIRTVHLLM--YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELRE 246
            S RT+ LL+  Y     +  ++GPKE++ L+  L  WR IF R+D DRSGK++S +LR+
Sbjct: 89  ISNRTIRLLLFIYKIPVDSLLRLGPKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRD 148

Query: 247 ALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGS 306
           A  +LG  +   V  L+VS+FD   GK+  + +D+F+EC + VKGLTEKF+E D  Y+G 
Sbjct: 149 AFYNLGCVLPTSVHQLIVSQFDDGTGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGY 208

Query: 307 ATFSYEAFMLTVLPFL 320
           AT SY+ FML V+PF+
Sbjct: 209 ATLSYDVFMLMVIPFI 224

[Close]

BLAST of MC03g1086 vs. ExPASy Swiss-Prot
Match: Q5PQ53 (Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.0e-28
Identity = 101/296 (34.12%), Postives = 147/296 (49.66%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPP--PTAQPYASSAYGGQQPPPHSQQSYAPV 84
           M+ YP     YG GY GA         G PP  P    Y    YGG   P  S    AP 
Sbjct: 1   MASYP-----YGQGYHGAA--------GQPPGAPQTNYYGGQQYGGGVQPAASYGRPAP- 60

Query: 85  SAPYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGN----P 144
            APYG+P  P G   G   P      + GG  GG       GG ++ P    YG+     
Sbjct: 61  GAPYGSP--PSGGVYGHPVPGSAAPGAPGGPYGGQ----APGGPYSVPGSTPYGSQQHGS 120

Query: 145 FASLLPSA-FPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYN-QKFSIRTVHLLMY 204
           +    P+   PPG DP   + FQ  D D SG+I  KEL+  L + N   F+  T  ++M 
Sbjct: 121 YGQGAPAGNIPPGVDPEAFSWFQTVDTDHSGYISLKELKQALVNTNWSSFNDETCTMMMN 180

Query: 205 HFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVV 264
            F  +N+ +I    F AL+  +Q WR +F+++D DRSG I+  EL +AL  +G+ +SP  
Sbjct: 181 MFDKSNSGRIDMFGFSALWRFIQQWRNLFQQYDRDRSGSINQGELHQALCQMGYQLSPQF 240

Query: 265 LDLLVSKFDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFM 313
           + +++S++ +   +   ++ D FI+ C  ++ +TE F+EKDT   G+A  SYE F+
Sbjct: 241 VQIVMSRYAQRSAQ-PGLQLDRFIQICTQLQSMTEAFREKDTGQIGTAKLSYEDFI 275

[Close]

BLAST of MC03g1086 vs. NCBI nr
Match: XP_022153892.1 (probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153893.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153894.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_022153895.1 probable calcium-binding protein CML49 isoform X1 [Momordica charantia])

HSP 1 Score: 590 bits (1521), Expect = 4.16e-212
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 144
           PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120

Query: 145 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 204
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 205 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 264
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240

Query: 265 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 297

[Close]

BLAST of MC03g1086 vs. NCBI nr
Match: XP_022966416.1 (probable calcium-binding protein CML49 [Cucurbita maxima])

HSP 1 Score: 522 bits (1344), Expect = 2.70e-185
Identity = 266/297 (89.56%), Postives = 275/297 (92.59%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYGGAG      GYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAG------GYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 144
           PYGAPSAPYGS+ G+KP KDKP+QS+GG GG HQSYGG    H YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGG----HGYPPPSAYGSPFASLLP 120

Query: 145 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 204
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 205 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 264
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKF 240

Query: 265 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of MC03g1086 vs. NCBI nr
Match: XP_023518609.1 (probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521 bits (1341), Expect = 7.72e-185
Identity = 265/297 (89.23%), Postives = 275/297 (92.59%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYGGAG      GYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAG------GYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 144
           PYGAPSAPYGS+ G+KP KDKP+QS+GG GG HQSYGG    H YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGG----HGYPPPSAYGSPFASLLP 120

Query: 145 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 204
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 205 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 264
           KIGPKEFIALFYGLQSWRGIFERFDSDRSG+ID NELREALLSLGFAVSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGRIDYNELREALLSLGFAVSPMVLDLLVSKF 240

Query: 265 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of MC03g1086 vs. NCBI nr
Match: XP_004142653.1 (probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetical protein Csa_011963 [Cucumis sativus])

HSP 1 Score: 518 bits (1333), Expect = 1.43e-183
Identity = 267/298 (89.60%), Postives = 277/298 (92.95%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYG  G      GYGAPPPTAQPY SSAYG   P   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGSTG------GYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAG-GGHAYPPPNSYGNPFASLL 144
           PYG PSAPYGS+ G+KPPKDKP+QSYGGAGGGHQSYGGA  GGH YPPP++YG+PFASLL
Sbjct: 61  PYGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLL 120

Query: 145 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 204
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 205 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 264
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240

Query: 265 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

[Close]

BLAST of MC03g1086 vs. NCBI nr
Match: XP_038883807.1 (probable calcium-binding protein CML50 [Benincasa hispida])

HSP 1 Score: 518 bits (1333), Expect = 1.59e-183
Identity = 270/298 (90.60%), Postives = 279/298 (93.62%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYG     GG GGYGAPPPTAQ Y SSAYG  QP   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGY----GGSGGYGAPPPTAQVYNSSAYGHPQPQ-QTQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAG-GGHAYPPPNSYGNPFASLL 144
           PYGAPSAPYGS+ G+KPPKDKP+QSYGGAGGGHQSYGGAG GGH YPPP++YGNPFASLL
Sbjct: 61  PYGAPSAPYGSAPGEKPPKDKPNQSYGGAGGGHQSYGGAGSGGHGYPPPSAYGNPFASLL 120

Query: 145 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 204
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQ VLSSY+QKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQRVLSSYDQKFSIRTVHLLMYHFTNTNT 180

Query: 205 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 264
           RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDS ELREALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSKELREALLSLGFAVSPMVLDLLVSK 240

Query: 265 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 293

[Close]

BLAST of MC03g1086 vs. ExPASy TrEMBL
Match: A0A6J1DIS2 (probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021303 PE=4 SV=1)

HSP 1 Score: 590 bits (1521), Expect = 2.01e-212
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA
Sbjct: 1   MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 144
           PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP
Sbjct: 61  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 120

Query: 145 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 204
           SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 205 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 264
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 240

Query: 265 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 297

[Close]

BLAST of MC03g1086 vs. ExPASy TrEMBL
Match: A0A6J1HP98 (probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC111466073 PE=4 SV=1)

HSP 1 Score: 522 bits (1344), Expect = 1.31e-185
Identity = 266/297 (89.56%), Postives = 275/297 (92.59%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYGGAG      GYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAG------GYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 144
           PYGAPSAPYGS+ G+KP KDKP+QS+GG GG HQSYGG    H YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPGEKPHKDKPNQSHGGVGGSHQSYGG----HGYPPPSAYGSPFASLLP 120

Query: 145 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 204
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 180

Query: 205 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 264
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKID NELREALLSLGFAVSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFAVSPMVLDLLVSKF 240

Query: 265 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of MC03g1086 vs. ExPASy TrEMBL
Match: A0A0A0L336 (Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 SV=1)

HSP 1 Score: 518 bits (1333), Expect = 6.91e-184
Identity = 267/298 (89.60%), Postives = 277/298 (92.95%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYG  G      GYGAPPPTAQPY SSAYG   P   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGSTG------GYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAG-GGHAYPPPNSYGNPFASLL 144
           PYG PSAPYGS+ G+KPPKDKP+QSYGGAGGGHQSYGGA  GGH YPPP++YG+PFASLL
Sbjct: 61  PYGVPSAPYGSAQGEKPPKDKPNQSYGGAGGGHQSYGGAASGGHGYPPPSAYGSPFASLL 120

Query: 145 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 204
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 205 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 264
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPMVLDLLVSK 240

Query: 265 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 290

[Close]

BLAST of MC03g1086 vs. ExPASy TrEMBL
Match: A0A6J1EBH1 (probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432514 PE=4 SV=1)

HSP 1 Score: 517 bits (1332), Expect = 8.79e-184
Identity = 263/297 (88.55%), Postives = 274/297 (92.26%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYGGAG      GYGAPPPTAQPY SS YG Q PPPHSQQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGGAG------GYGAPPPTAQPYGSSPYGSQHPPPHSQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGAGGGHAYPPPNSYGNPFASLLP 144
           PYGAPSAPYGS+  +KP KDKP+QS+GG GG HQSYGG    H YPPP++YG+PFASLLP
Sbjct: 61  PYGAPSAPYGSNPSEKPHKDKPNQSHGGVGGSHQSYGG----HGYPPPSAYGSPFASLLP 120

Query: 145 SAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTR 204
           SAFPPGTDPSIV CFQVADQDGSGFIDDKELQGVLSSYNQ+FSIRTVHLLMYHFTNTNTR
Sbjct: 121 SAFPPGTDPSIVTCFQVADQDGSGFIDDKELQGVLSSYNQRFSIRTVHLLMYHFTNTNTR 180

Query: 205 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKF 264
           KIGPKEFIALFYGLQSWRGIFERFDSDRSGKID NELREALLSLGF+VSP+VLDLLVSKF
Sbjct: 181 KIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDYNELREALLSLGFSVSPMVLDLLVSKF 240

Query: 265 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 DKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 287

[Close]

BLAST of MC03g1086 vs. ExPASy TrEMBL
Match: A0A5D3BYW0 (Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold527G00390 PE=4 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 2.91e-183
Identity = 269/298 (90.27%), Postives = 279/298 (93.62%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYPNQPSGYGYGYG     GG GGYGAPPPTAQPY SSAYG   P   +QQ YAPV+A
Sbjct: 1   MSGYPNQPSGYGYGYGY----GGTGGYGAPPPTAQPYNSSAYG--HPQSQTQQPYAPVAA 60

Query: 85  PYGAPSAPYGSSTGDKPPKDKPHQSYGGAGGGHQSYGGA-GGGHAYPPPNSYGNPFASLL 144
           PYG PSAPY S+ G+KPPKDKP+QSYGGAGGGHQSYGGA GGGH YPPP++YGNPFASLL
Sbjct: 61  PYGGPSAPY-SAQGEKPPKDKPNQSYGGAGGGHQSYGGAAGGGHGYPPPSAYGNPFASLL 120

Query: 145 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 204
           PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT
Sbjct: 121 PSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNT 180

Query: 205 RKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSK 264
           RKIGPKEFI+LFYGLQSWRGIFERFDSDRSGKIDSNELR+ALLSLGFAVSP+VLDLLVSK
Sbjct: 181 RKIGPKEFISLFYGLQSWRGIFERFDSDRSGKIDSNELRDALLSLGFAVSPMVLDLLVSK 240

Query: 265 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 321
           FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA
Sbjct: 241 FDKSGGKSKAIEYDNFIECCLTVKGLTEKFKEKDTTYSGSATFSYEAFMLTVLPFLIA 291

[Close]

BLAST of MC03g1086 vs. TAIR 10
Match: AT5G04170.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 367.9 bits (943), Expect = 8.6e-102
Identity = 220/366 (60.11%), Postives = 242/366 (66.12%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYGYGG            PPP   PY+S   GG  PP  S  + +P + 
Sbjct: 1   MSGYPPTSQGYGYGYGGG---------NQPPPPQPPYSS---GGNNPPYGSSTTSSPYAV 60

Query: 85  PYGA----------------------PSAPYGSSTGD--KPPKDKP-------------- 144
           PYGA                      PSAPY  S GD  KPPK+KP              
Sbjct: 61  PYGASKPQSSSSSAPTYGSSSYGAPPPSAPYAPSPGDYNKPPKEKPYGGGYGAPPPSGSS 120

Query: 145 -HQSYG---------GAGGG-----------HQSYGGA---------GGGH-AYPPPNSY 204
            + SYG         G GGG           + SYGGA         GGG+  YPP  SY
Sbjct: 121 DYGSYGAGPRPSQPSGHGGGYGATPPHGVSDYGSYGGAPPRPASSGHGGGYGGYPPQASY 180

Query: 205 GNPFASLLPSAFPPGTDPSIVACFQVADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLM 264
           G+PFASL+PS F PGTDP+IVACFQ ADQDGSGFIDDKELQG LSSY Q+FS+RTVHLLM
Sbjct: 181 GSPFASLIPSGFAPGTDPNIVACFQAADQDGSGFIDDKELQGALSSYQQRFSMRTVHLLM 240

Query: 265 YHFTNTNTRKIGPKEFIALFYGLQSWRGIFERFDSDRSGKIDSNELREALLSLGFAVSPV 322
           Y FTN+N  KIGPKEF ALFY LQ+WR IFER D DRSG+ID NELR+ALLSLGF+VSPV
Sbjct: 241 YLFTNSNAMKIGPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPV 300

[Close]

BLAST of MC03g1086 vs. TAIR 10
Match: AT3G10300.3 (Calcium-binding EF-hand family protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.3e-101
Identity = 213/340 (62.65%), Postives = 238/340 (70.00%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 85  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 144
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 145 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 204
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 205 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 264
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 265 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 322
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 300

[Close]

BLAST of MC03g1086 vs. TAIR 10
Match: AT3G10300.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.4e-81
Identity = 179/300 (59.67%), Postives = 202/300 (67.33%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 85  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 144
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 145 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 204
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 205 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 264
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 265 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 282
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

[Close]

BLAST of MC03g1086 vs. TAIR 10
Match: AT3G10300.4 (Calcium-binding EF-hand family protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.4e-81
Identity = 179/300 (59.67%), Postives = 202/300 (67.33%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 85  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 144
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 145 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 204
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 205 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIGPKEFIALFYGLQSW 264
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIGPKEF +LF+ LQ+W
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGPKEFTSLFFSLQNW 240

Query: 265 RGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFIE 282
           R IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFIE
Sbjct: 241 RSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFIE 295

[Close]

BLAST of MC03g1086 vs. TAIR 10
Match: AT3G10300.5 (Calcium-binding EF-hand family protein )

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-79
Identity = 179/301 (59.47%), Postives = 202/301 (67.11%), Query Frame = 0


Query: 25  MSGYPNQPSGYGYGYGGAGAGGGYGGYGAPPPTAQPYASSAYGGQQPPPHSQQSYAPVSA 84
           MSGYP    GYGYG         YG  G  PP   PY SS  G   PPP+   + +P + 
Sbjct: 1   MSGYPPSSQGYGYGGNPPPPQPPYGSTGNNPP---PYGSS--GSNPPPPYGSSASSPYAV 60

Query: 85  PYGA--------PSAPYGSSTGD--KPPKDKPH--------------------QSYGGAG 144
           PYGA        PSAPY S  GD  KP K+KPH                      YGG G
Sbjct: 61  PYGAQPAPYGAPPSAPYASLPGDHNKPHKEKPHGASYGSPSPGGYGAHPSSGPSDYGGYG 120

Query: 145 GGHQ------SYGGA------GGGHAYPPPN-SYGNPFASLLPSAFPPGTDPSIVACFQV 204
           G  Q       YGGA      GGG+  PPP  SYG+PFASL+PSAFPPGTDP+IVACFQ 
Sbjct: 121 GAPQQSGHGGGYGGAPQQSGHGGGYGAPPPQASYGSPFASLVPSAFPPGTDPNIVACFQA 180

Query: 205 ADQDGSGFIDDKELQGVLSSYNQKFSIRTVHLLMYHFTNTNTRKIG-PKEFIALFYGLQS 264
           AD+D SGFIDDKELQG LSSYNQ FSIRTVHLLMY FTN+N RKIG PKEF +LF+ LQ+
Sbjct: 181 ADRDNSGFIDDKELQGALSSYNQSFSIRTVHLLMYLFTNSNVRKIGRPKEFTSLFFSLQN 240

Query: 265 WRGIFERFDSDRSGKIDSNELREALLSLGFAVSPVVLDLLVSKFDKSGGKSKAIEYDNFI 282
           WR IFERFD DRSG+ID+NELR+AL+SLGF+VSPV+LDLLVSKFDKSGG+++AIEYDNFI
Sbjct: 241 WRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDKSGGRNRAIEYDNFI 296

The following BLAST results are available for this feature:

BLAST of MC03g1086 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYE4	1.2e-100	60.11	Probable calcium-binding protein CML50 OS=Arabidopsis thaliana OX=3702 GN=CML50 ...	[more]
Q8W4L0	4.6e-100	62.65	Probable calcium-binding protein CML49 OS=Arabidopsis thaliana OX=3702 GN=CML49 ...	[more]
Q2QY10	5.8e-95	65.05	Calcium-binding protein CBP OS=Oryza sativa subsp. japonica OX=39947 GN=CBP PE=2...	[more]
Q9ZQH1	3.0e-35	42.35	Probable calcium-binding protein CML48 OS=Arabidopsis thaliana OX=3702 GN=CML48 ...	[more]
Q5PQ53	8.0e-28	34.12	Peflin OS=Xenopus laevis OX=8355 GN=pef1 PE=1 SV=1	[more]

BLAST of MC03g1086 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153892.1	4.16e-212	100.00	probable calcium-binding protein CML49 isoform X1 [Momordica charantia] >XP_0221...	[more]
XP_022966416.1	2.70e-185	89.56	probable calcium-binding protein CML49 [Cucurbita maxima]	[more]
XP_023518609.1	7.72e-185	89.23	probable calcium-binding protein CML49 [Cucurbita pepo subsp. pepo]	[more]
XP_004142653.1	1.43e-183	89.60	probable calcium-binding protein CML49 [Cucumis sativus] >KGN54506.1 hypothetica...	[more]
XP_038883807.1	1.59e-183	90.60	probable calcium-binding protein CML50 [Benincasa hispida]	[more]

BLAST of MC03g1086 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIS2	2.01e-212	100.00	probable calcium-binding protein CML49 isoform X1 OS=Momordica charantia OX=3673...	[more]
A0A6J1HP98	1.31e-185	89.56	probable calcium-binding protein CML49 OS=Cucurbita maxima OX=3661 GN=LOC1114660...	[more]
A0A0A0L336	6.91e-184	89.60	Ef-hand calcium binding protein OS=Cucumis sativus OX=3659 GN=Csa_4G341560 PE=4 ...	[more]
A0A6J1EBH1	8.79e-184	88.55	probable calcium-binding protein CML49 isoform X1 OS=Cucurbita moschata OX=3662 ...	[more]
A0A5D3BYW0	2.91e-183	90.27	Putative calcium-binding protein CML49 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of MC03g1086 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04170.1	8.6e-102	60.11	Calcium-binding EF-hand family protein	[more]
AT3G10300.3	3.3e-101	62.65	Calcium-binding EF-hand family protein	[more]
AT3G10300.2	6.4e-81	59.67	Calcium-binding EF-hand family protein	[more]
AT3G10300.4	6.4e-81	59.67	Calcium-binding EF-hand family protein	[more]
AT3G10300.5	1.6e-79	59.47	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 154..182 e-value: 0.092 score: 21.9 coord: 220..248 e-value: 0.0097 score: 25.1
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 221..249 e-value: 2.5E-5 score: 23.7
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 155..178 e-value: 6.6E-5 score: 22.2
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 216..251 score: 12.867577
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 150..185 score: 10.691735
None	No IPR available	GENE3D	1.10.238.10		coord: 128..320 e-value: 1.4E-39 score: 137.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..129
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..83
None	No IPR available	PANTHER	PTHR46824:SF1	CALCIUM-BINDING PROTEIN CML49-RELATED	coord: 25..321
None	No IPR available	CDD	cd16180	EFh_PEF_Group_I	coord: 154..318 e-value: 5.92362E-76 score: 228.182
IPR044590	Probable calcium-binding protein CML48/49/50	PANTHER	PTHR46824	CALCIUM-BINDING PROTEIN CML48-RELATED	coord: 25..321
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 229..241
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 163..175
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 149..316

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g1086.1	MC03g1086.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding