MC03g1020 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g1020 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 526/804 (65.42%), Postives = 642/804 (79.85%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDS-NFVANGHHPILTHVPPYI----TAT 60
           MAPPS+TK A   +V+  ++  + P LSI+L  S NF+ NG HP LT VPP I    T+T
Sbjct: 1   MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSRNFLVNG-HPFLTQVPPNITTTTTST 60

Query: 61  PSPLI----------SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVW 120
           PSP +          +   T    GCFVGF+  E+ S HV  +G+L+GI+F+SIFRFKVW
Sbjct: 61  PSPFLDFKSNKDTIANNNNTLQQQGCFVGFNTTEAKSHHVVPLGKLKGIKFTSIFRFKVW 120

Query: 121 WTTQWVGTCGSDVQHETQMMILDRS-DQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMW 180
           WTT WVGT G ++QHETQ++ILD++   GRP+VLLLPILE +FR SL+PG   +D V M 
Sbjct: 121 WTTHWVGTNGHELQHETQILILDKNISLGRPYVLLLPILENSFRTSLQPG--LNDYVDMS 180

Query: 181 VESGSTCVRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGW 240
           VESGST V  S F++CLY+ +  DPY LVK+A+K ++  LGTFK LEEKTPP I++KFGW
Sbjct: 181 VESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQTKLGTFKTLEEKTPPSIIEKFGW 240

Query: 241 CTWDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLT 300
           CTWDAFYLKV+P+G+  GVK L DGGCPPG V+IDDGWQSI+HD D      E++ M+ T
Sbjct: 241 CTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGWQSISHDDDDPV--TERDGMNRT 300

Query: 301 TAGEQMPCRLIKFEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYW 360
           +AGEQMPCRLIK+EENYKFREY++ D    KGL  FV+DLKEEFRSVE VYVWHALCGYW
Sbjct: 301 SAGEQMPCRLIKYEENYKFREYENGDNGGKKGLVGFVRDLKEEFRSVESVYVWHALCGYW 360

Query: 361 GGIRPNVLGMPLSRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHL 420
           GG+RP V GMP ++++ P LS  ++MTMEDLAVDKIV +G+GLVPP LA +M++G+HSHL
Sbjct: 361 GGVRPKVCGMPEAKVVVPKLSPGVKMTMEDLAVDKIVENGVGLVPPNLAQEMFDGIHSHL 420

Query: 421 QSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDF 480
           +SAGIDGVKVDVIHLLE+LSEE+GGR+ELAKAYY ALT S++K+FKGNGVIASMEHCNDF
Sbjct: 421 ESAGIDGVKVDVIHLLELLSEEYGGRVELAKAYYKALTSSVNKHFKGNGVIASMEHCNDF 480

Query: 481 MYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQS 540
             LGTEAI+LGRVGDDFW  D SGDP   YWLQGCHMVHCAYNSLWMGN IHPDWDMFQS
Sbjct: 481 FLLGTEAISLGRVGDDFWCCDPSGDPNGTYWLQGCHMVHCAYNSLWMGNFIHPDWDMFQS 540

Query: 541 NHPCAEFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFED 600
            HPCAEFHAASRAISGGP+Y+SD VG+HNF LLKS VLPDGSILRCQ++ALPTRDCLFED
Sbjct: 541 THPCAEFHAASRAISGGPVYVSDCVGNHNFKLLKSFVLPDGSILRCQHYALPTRDCLFED 600

Query: 601 PLHDGKTILKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWN 660
           PLH+GKT+LK WNLNK+ G LGLFNCQGGGWCP+TR+N+ ASE++  +TC A P+DIEW 
Sbjct: 601 PLHNGKTMLKIWNLNKYAGVLGLFNCQGGGWCPETRRNKSASEFSHAVTCYASPEDIEWC 660

Query: 661 NGKTPISVKGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGF 720
           NGKTP+ +KGV+ FA+Y  ++KKL L+K S+ LE S+ P +++L+ VSP+ V SK  + F
Sbjct: 661 NGKTPMDIKGVDVFAVYFFKEKKLSLMKCSDRLEVSLEPFSFELMTVSPLKVFSKRLIQF 720

Query: 721 APIGLANMLNPGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYE 780
           APIGL NMLN GGA+QSLE D++  LV++G RGCGE+ VFASE P  CKIDG  V F+YE
Sbjct: 721 APIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSVFASEKPVCCKIDGVSVEFDYE 780

Query: 781 DDQMVKIQVPWPGSSRLSIIEYRF 785
            D+MV++Q+ WPGSS LS++E+ F
Sbjct: 781 -DKMVRVQILWPGSSTLSLVEFLF 798

BLAST of MC03g1020 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 1.6e-295
Identity = 489/800 (61.12%), Postives = 604/800 (75.50%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MA P LTK   +D  +  ++ + +      L+DS  +ANG   +LT VP  +T T SP +
Sbjct: 1   MASPCLTK---SDSGINGVDFTEK----FRLEDSTLLANG-QVVLTDVPVNVTLTSSPYL 60

Query: 61  SETE---TQLAVGCFVGFDAD-ESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
            + +     ++ G F+GF+ D E  S HVASIG+L+ IRF SIFRFKVWWTT WVG+ G 
Sbjct: 61  VDKDGVPLDVSAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGR 120

Query: 121 DVQHETQMMILDR--------SDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESG 180
           D+++ETQ++ILD+        S  GRP+VLLLP+LEG+FR S + G   DD VA+ VESG
Sbjct: 121 DIENETQIIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSG--EDDDVAVCVESG 180

Query: 181 STCVRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWD 240
           ST V  S FR  +Y+  G+DP+ LVKDAMK +++ + TFKLLEEK+PPGI+DKFGWCTWD
Sbjct: 181 STEVTGSEFRQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWD 240

Query: 241 AFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGE 300
           AFYL VNP G+  GVKCLVDGGCPPG+VLIDDGWQSI HD+D I    + E M++T AGE
Sbjct: 241 AFYLTVNPDGVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGI----DVEGMNITVAGE 300

Query: 301 QMPCRLIKFEENYKFREYKSDR-ENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIR 360
           QMPCRL+KFEEN+KF++Y S + +N  G+ AFV+DLK+EF +V+Y+YVWHALCGYWGG+R
Sbjct: 301 QMPCRLLKFEENHKFKDYVSPKDQNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLR 360

Query: 361 PNVLGMPLSRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAG 420
           P    +P S II P LS  L++TMEDLAVDKI+ +GIG   P+LA + YEGLHSHLQ+AG
Sbjct: 361 PEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAG 420

Query: 421 IDGVKVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLG 480
           IDGVKVDVIH+LEML +++GGR++LAKAY+ ALT S++K+F GNGVIASMEHCNDFM+LG
Sbjct: 421 IDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLG 480

Query: 481 TEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPC 540
           TEAI+LGRVGDDFW  D SGDP   +WLQGCHMVHCAYNSLWMGN I PDWDMFQS HPC
Sbjct: 481 TEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPC 540

Query: 541 AEFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHD 600
           AEFHAASRAISGGPIYISD VG H+F+LLK LVLP+GSILRC+Y+ALPTRD LFEDPLHD
Sbjct: 541 AEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHD 600

Query: 601 GKTILKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKT 660
           GKT+LK WNLNK+TG +G FNCQGGGWC +TR+N+  SE   TLT    PKD+EWN+G +
Sbjct: 601 GKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSS 660

Query: 661 PISVKGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIG 720
           PIS+  V  FA+++ + KKL L   +++LE ++ P  ++L+ VSPV  +    V FAPIG
Sbjct: 661 PISIANVEEFALFLSQSKKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIG 720

Query: 721 LANMLNPGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQM 780
           L NMLN  GAI+SL    N+  V VG  G GE  V+AS+ P SC IDGE V F YE D M
Sbjct: 721 LVNMLNTSGAIRSLVY--NDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSM 780

Query: 781 VKIQVPWPGSSRLSIIEYRF 785
           V +QVPW G   LS I+Y F
Sbjct: 781 VMVQVPWSGPDGLSSIQYLF 783

BLAST of MC03g1020 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 2.9e-281
Identity = 470/799 (58.82%), Postives = 586/799 (73.34%), Query Frame = 0

Query: 1   MAPP-SLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATP-SP 60
           MAP  S  K+ +  +VV V      P    TLK  +   +G HP L  VP  I  TP S 
Sbjct: 1   MAPNLSKAKDDLIGDVVAVDGLIKPP--RFTLKGKDLAVDG-HPFLLDVPANIRLTPAST 60

Query: 61  LISETET-QLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSD 120
           L+  ++    A G F+GFDA  +   HV  IG+LR  RF SIFRFKVWWTT WVGT G D
Sbjct: 61  LVPNSDVPAAAAGSFLGFDAPAAKDRHVVPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRD 120

Query: 121 VQHETQMMILDRSDQ------GRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTC 180
           V++ETQMMILD+S         RP+VLLLPI+EG FR  L   G  +D V M +ESGS+ 
Sbjct: 121 VENETQMMILDQSGTKSSPTGPRPYVLLLPIVEGPFRACLE-SGKAEDYVHMVLESGSST 180

Query: 181 VRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFY 240
           VR S FRS +Y+  G+DP+ LVKDAM+ V+  LGTF+L+EEKTPP I+DKFGWCTWDAFY
Sbjct: 181 VRGSVFRSAVYLHAGDDPFDLVKDAMRVVRAHLGTFRLMEEKTPPPIVDKFGWCTWDAFY 240

Query: 241 LKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMP 300
           LKV+P+G+  GV+ L DGGCPPG+VLIDDGWQSI HD D +  G   E M+ T+AGEQMP
Sbjct: 241 LKVHPEGVWEGVRRLADGGCPPGLVLIDDGWQSICHDDDDLGSG--AEGMNRTSAGEQMP 300

Query: 301 CRLIKFEENYKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVL 360
           CRLIKF+ENYKFREYK       G+G FV+++K  F +VE VYVWHALCGYWGG+RP   
Sbjct: 301 CRLIKFQENYKFREYKG------GMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAP 360

Query: 361 GMPLSRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGV 420
           G+P +++++P LS  L+ TMEDLAVDKIV++G+GLV P  A ++YEGLHSHLQ++GIDGV
Sbjct: 361 GLPPAKVVAPRLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGV 420

Query: 421 KVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAI 480
           KVDVIHLLEM+ EE+GGR+ELAKAY+  LT S+ ++F GNGVIASMEHCNDFM LGTEA+
Sbjct: 421 KVDVIHLLEMVCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAV 480

Query: 481 TLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFH 540
            LGRVGDDFW  D SGDP   +WLQGCHMVHCAYNSLWMG  IHPDWDMFQS HPCA FH
Sbjct: 481 ALGRVGDDFWCTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFH 540

Query: 541 AASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTI 600
           AASRA+SGGP+Y+SD+VG H+F+LL+ L LPDG+ILRC+ +ALPTRDCLF DPLHDGKT+
Sbjct: 541 AASRAVSGGPVYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTM 600

Query: 601 LKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISV 660
           LK WN+NKF+G LG FNCQGGGW  + R+N  A+ ++  +T  A P D+EW++G      
Sbjct: 601 LKIWNVNKFSGVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGG----- 660

Query: 661 KGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPY--VGFAPIGLA 720
            G + FA+Y V  +KL+LL+  E++E ++ P  Y+LLVV+PV  +  P   +GFAPIGLA
Sbjct: 661 GGGDRFAVYFVEARKLQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLA 720

Query: 721 NMLNPGGAIQSLEIDENEGLV--RVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQM 780
           NMLN GGA+Q  E    +G V   V  +G GE++ ++S  PR CK++G+D  F+YED  +
Sbjct: 721 NMLNAGGAVQGFEAARKDGDVAAEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYEDG-I 780

Query: 781 VKIQVPWPGSS-RLSIIEY 783
           V + VPW GSS +LS +EY
Sbjct: 781 VTVDVPWTGSSKKLSRVEY 781

BLAST of MC03g1020 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 681.4 bits (1757), Expect = 1.2e-194
Identity = 360/863 (41.71%), Postives = 498/863 (57.71%), Query Frame = 0

Query: 1   MAPP--SLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVP--------- 60
           MAPP  S T N I  E +              L +  F   G  P+   VP         
Sbjct: 1   MAPPLNSTTSNLIKTESI------------FDLSERKFKVKG-FPLFHDVPENVSFRSFS 60

Query: 61  ----PYITATPSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVW 120
               P  +  P  L+ +       G F GF  +  +   + SIG   G  F SIFRFK W
Sbjct: 61  SICKPSESNAPPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTW 120

Query: 121 WTTQWVGTCGSDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWV 180
           W+TQW+G  GSD+Q ETQ ++++   + + +V+++PI+E  FR +L PG   +D V +  
Sbjct: 121 WSTQWIGKSGSDLQMETQWILIE-VPETKSYVVIIPIIEKCFRSALFPG--FNDHVKIIA 180

Query: 181 ESGSTCVRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWC 240
           ESGST V+ S F S  Y+   E+PY L+K+A  A+++ L +F+LLEEKT P ++DKFGWC
Sbjct: 181 ESGSTKVKESTFNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWC 240

Query: 241 TWDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTT 300
           TWDAFYL VNP GI  G+     GG  P  V+IDDGWQSI+ D         ++A +L  
Sbjct: 241 TWDAFYLTVNPIGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYD----PNEDAKNLVL 300

Query: 301 AGEQMPCRLIKFEENYKFREY--------------------------------------- 360
            GEQM  RL +F+E YKFR+Y                                       
Sbjct: 301 GGEQMSGRLHRFDECYKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAI 360

Query: 361 ----------------------------------KSDRENCKGLGAFVKDLKEEFRSVEY 420
                                             KS+ ++  GL AF KDL+ +F+ ++ 
Sbjct: 361 SSKSSDLAEIESKIKKVVKEIDDLFGGEQFSSGEKSEMKSEYGLKAFTKDLRTKFKGLDD 420

Query: 421 VYVWHALCGYWGGIRPNVLGMPLSRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELA 480
           VYVWHALCG WGG+RP    +  ++I+   LS  L+ TMEDLAV +I  + +GLV P  A
Sbjct: 421 VYVWHALCGAWGGVRPETTHLD-TKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQA 480

Query: 481 HKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNG 540
           +++Y+ +HS+L  +GI GVKVDVIH LE + +E+GGR++LAK YY  LT SI K F GNG
Sbjct: 481 NELYDSMHSYLAESGITGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNG 540

Query: 541 VIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGN 600
           +IASM+HCNDF +LGT+ I++GRVGDDFW +D +GDP   +WLQG HM+HC+YNSLWMG 
Sbjct: 541 MIASMQHCNDFFFLGTKQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQ 600

Query: 601 MIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYH 660
           MI PDWDMFQS+H CA+FHA SRAI GGPIY+SD+VGSH+F+L+K LV PDG+I +C Y 
Sbjct: 601 MIQPDWDMFQSDHVCAKFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYF 660

Query: 661 ALPTRDCLFEDPLHDGKTILKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLT 720
            LPTRDCLF++PL D  T+LK WN NK+ G +G FNCQG GW P  ++ R   E  + + 
Sbjct: 661 PLPTRDCLFKNPLFDHTTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIP 720

Query: 721 CVAGPKDIEWNNGKTPISVKGVNFFAIYMVRQKKLELLK-ASENLEYSIAPLNYQLLVVS 772
                 ++EW+  +    +     + +Y+ + ++L L+   SE ++++I P  ++L    
Sbjct: 721 GTVHVTEVEWDQKEETSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFV 780

BLAST of MC03g1020 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 668.3 bits (1723), Expect = 1.1e-190
Identity = 362/872 (41.51%), Postives = 499/872 (57.22%), Query Frame = 0

Query: 1   MAPPSLTKNAIND--EVVGVLNASSRPAL---SITLKDSNFVANGHHPILTHVPPYITAT 60
           MAP   + ++IND  E   +    ++P L   S  L + +  A    PIL  VP  +T T
Sbjct: 1   MAPLHESLSSINDVIESKPLFVPITKPILQPNSFNLSEGSLCAKDSTPILFDVPQNVTFT 60

Query: 61  -----------PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKV 120
                      P P++   +     G F+GF  +  +     S+GR     F S+FRFK+
Sbjct: 61  PFSSHSISTDAPLPILLRVQANAHKGGFLGFTKESPSDRLTNSLGRFEDREFLSLFRFKM 120

Query: 121 WWTTQWVGTCGSDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMW 180
           WW+T W+G  GSD+Q ETQ ++L +  +   +V ++P +EGAFR SL PG  G+  V + 
Sbjct: 121 WWSTAWIGKSGSDLQAETQWVML-KIPEIDSYVAIIPTIEGAFRASLTPGEKGN--VLIC 180

Query: 181 VESGSTCVRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGW 240
            ESGST V+ S F+S  YI + ++PY+L+K+A  A+++ + TFKLLEEK  P I+DKFGW
Sbjct: 181 AESGSTKVKESSFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGW 240

Query: 241 CTWDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLT 300
           CTWDA YL V+P  I TGVK   DGG  P  V+IDDGWQSI  D D +    +++A +L 
Sbjct: 241 CTWDACYLTVDPATIWTGVKEFEDGGVCPKFVIIDDGWQSINFDGDEL----DKDAENLV 300

Query: 301 TAGEQMPCRLIKFEENYKFREYK------------------------------------- 360
             GEQM  RL  F+E  KFR YK                                     
Sbjct: 301 LGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPLKPKMLIYKATERIQAIILRRKL 360

Query: 361 -------------------------------------SDRENCKGLGAFVKDLKEEFRSV 420
                                                SD  +  G+ AF KDL+  F+S+
Sbjct: 361 VKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGSDDVSGSGMAAFTKDLRLRFKSL 420

Query: 421 EYVYVWHALCGYWGGIRPNVLGMPLSRIISP-NLSQELEMTMEDLAVDKIVSSGIGLVPP 480
           + +YVWHALCG W G+RP  + M L   ++P  LS  L  TM DLAVDK+V +GIGLV P
Sbjct: 421 DDIYVWHALCGAWNGVRPETM-MDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHP 480

Query: 481 ELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFK 540
             AH+ Y+ +HS+L S G+ G K+DV   LE L+EE GGR+ELAKAYY  LT S+ K F 
Sbjct: 481 SKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFN 540

Query: 541 GNGVIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLW 600
           G  VIASM+ CN+F +L T+ I++GRVGDDFW +D  GDP   YWLQG HM+HC+YNS+W
Sbjct: 541 GTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIW 600

Query: 601 MGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDSVG--SHNFNLLKSLVLPDGSIL 660
           MG MI PDWDMFQS+H CAE+HAASRAI GGP+Y+SD +G  SHNF+L+K L   DG+I 
Sbjct: 601 MGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIP 660

Query: 661 RCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEY 720
           RC ++ALPTRD LF++PL D ++ILK +N NKF G +G FNCQG GW P+  + +   E 
Sbjct: 661 RCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKEC 720

Query: 721 ARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMV-RQKKLELL---KASENLEYSIAPL 772
             T++      DIEW+        + V +   Y+V +Q+  E+L     SE ++ ++ P 
Sbjct: 721 YTTVSGTVHVSDIEWDQNPEAAGSQ-VTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPS 780

BLAST of MC03g1020 vs. NCBI nr
Match: XP_022137045.1 (galactinol--sucrose galactosyltransferase-like [Momordica charantia])

HSP 1 Score: 1633 bits (4228), Expect = 0.0
Identity = 784/784 (100.00%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI
Sbjct: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60

Query: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120
           SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH
Sbjct: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120

Query: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180
           ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC
Sbjct: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180

Query: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240
           LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR
Sbjct: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240

Query: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300
           TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN
Sbjct: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300

Query: 301 YKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS 360
           YKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS
Sbjct: 301 YKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS 360

Query: 361 PNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLE 420
           PNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLE
Sbjct: 361 PNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLE 420

Query: 421 MLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDF 480
           MLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDF
Sbjct: 421 MLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDF 480

Query: 481 WTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIY 540
           WTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIY
Sbjct: 481 WTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIY 540

Query: 541 ISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGA 600
           ISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGA
Sbjct: 541 ISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGA 600

Query: 601 LGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVR 660
           LGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVR
Sbjct: 601 LGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVR 660

Query: 661 QKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEI 720
           QKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEI
Sbjct: 661 QKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEI 720

Query: 721 DENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSSRLSII 780
           DENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSSRLSII
Sbjct: 721 DENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSSRLSII 780

Query: 781 EYRF 784
           EYRF
Sbjct: 781 EYRF 784

BLAST of MC03g1020 vs. NCBI nr
Match: XP_038894030.1 (galactinol--sucrose galactosyltransferase-like [Benincasa hispida])

HSP 1 Score: 1326 bits (3433), Expect = 0.0
Identity = 638/786 (81.17%), Postives = 700/786 (89.06%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLTKNAIN  ++  L  +    LSITLK S F+ANGH PILTHVP  I +TPSP  
Sbjct: 1   MAPPSLTKNAIN--IIPSLLDTPSSNLSITLKGSEFLANGH-PILTHVPSNIISTPSPF- 60

Query: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120
              +T  ++GCFVGFDA+E  S H+A IG L  I+FSS+FRFKVWWTT W GTCG D+QH
Sbjct: 61  ---KTNNSLGCFVGFDANEPRSSHLAPIGNLTDIKFSSLFRFKVWWTTHWAGTCGRDIQH 120

Query: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180
           ETQ+MILD +  GRPFVLLLPILEGAFRCSLRPG D DD+VAMWVESGST V+ASRFRSC
Sbjct: 121 ETQIMILDTNHHGRPFVLLLPILEGAFRCSLRPGDDHDDSVAMWVESGSTTVQASRFRSC 180

Query: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240
           LY+QVGEDPY+LVK+AM+ VKL LGTFKLL++KTPP I+DKFGWCTWDAFYLKVNPQGIR
Sbjct: 181 LYMQVGEDPYTLVKEAMEVVKLHLGTFKLLQDKTPPPIVDKFGWCTWDAFYLKVNPQGIR 240

Query: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300
           TGVKCLVDGGCPPGM+LIDDGWQSIAHDADSITD   QEAMDLT AGEQMPCRLIKFEEN
Sbjct: 241 TGVKCLVDGGCPPGMILIDDGWQSIAHDADSITD-HHQEAMDLTAAGEQMPCRLIKFEEN 300

Query: 301 YKFREYKSDRENCK-GLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRII 360
           YKFR+Y+S  +    G+G FV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMPLSR+I
Sbjct: 301 YKFRDYESPGKGFDVGMGGFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMPLSRVI 360

Query: 361 SPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLL 420
           +P LSQ LE TMEDLAVDKIV++GIGLVPPELAHKMY+GLHSHLQSAGIDGVKVDVIHLL
Sbjct: 361 TPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHKMYDGLHSHLQSAGIDGVKVDVIHLL 420

Query: 421 EMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDD 480
           EMLSEEFGGRIELAKAYY ALT SI K+ +GNGVIASMEHCNDFMYLGTEAI LGRVGDD
Sbjct: 421 EMLSEEFGGRIELAKAYYKALTSSIKKHLQGNGVIASMEHCNDFMYLGTEAIALGRVGDD 480

Query: 481 FWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPI 540
           FWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAISGGPI
Sbjct: 481 FWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSMHPCAEFHAASRAISGGPI 540

Query: 541 YISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTG 600
           Y+SDSVG HNF LLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LK WNLNKFTG
Sbjct: 541 YVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNLNKFTG 600

Query: 601 ALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMV 660
           ALGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNG+ PIS+KGVN FAIYMV
Sbjct: 601 ALGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGENPISLKGVNLFAIYMV 660

Query: 661 RQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLE 720
           R +KL+LLK SENLE++IAPLNY+LLVVSPVT+LSKP + FAPIGL NMLN GGAIQSLE
Sbjct: 661 RDEKLKLLKTSENLEFTIAPLNYELLVVSPVTILSKPNMEFAPIGLVNMLNCGGAIQSLE 720

Query: 721 IDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDD-QMVKIQVPWPGSSRLS 780
           IDENEGLV  G +GCGE+ VFAS+ PR+CKIDG+DV FEY+DD +MVKIQVPWP SSRLS
Sbjct: 721 IDENEGLVTAGVKGCGEMRVFASKEPRNCKIDGKDVEFEYDDDDKMVKIQVPWPSSSRLS 778

Query: 781 IIEYRF 784
           IIEYRF
Sbjct: 781 IIEYRF 778

BLAST of MC03g1020 vs. NCBI nr
Match: XP_023519339.1 (galactinol--sucrose galactosyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1316 bits (3406), Expect = 0.0
Identity = 640/789 (81.12%), Postives = 699/789 (88.59%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLT N I D+V+ +L+A S   LSI LK S FVANGH P LTHVP  ITAT S LI
Sbjct: 1   MAPPSLTSNPI-DDVIPLLDAPSSD-LSIALKGSEFVANGH-PFLTHVPSNITATSSQLI 60

Query: 61  SETETQ----LAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
              +T     L  GCF+GF+A E  SCH+  IG LRGIRFSSIFRFKVWWTT WVG+CG 
Sbjct: 61  FSHKTTIPKPLLGGCFLGFNAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWVGSCGG 120

Query: 121 DVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASR 180
           D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPGGD  DTVAMWVESGST V+AS+
Sbjct: 121 DIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPGGD--DTVAMWVESGSTTVQASQ 180

Query: 181 FRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNP 240
           FRSCLY+QVG+DPY+LV +AM  VKL LGTFKLL++KTPP I+DKFGWCTWDAFYLKVNP
Sbjct: 181 FRSCLYMQVGKDPYTLVNEAMNVVKLHLGTFKLLDDKTPPPIVDKFGWCTWDAFYLKVNP 240

Query: 241 QGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIK 300
            G+RTGVKCLVDGGCPPGM+LIDDGWQSIAHDADSI D   QEAMDLT AGEQMPCRLIK
Sbjct: 241 HGVRTGVKCLVDGGCPPGMILIDDGWQSIAHDADSIADPH-QEAMDLTVAGEQMPCRLIK 300

Query: 301 FEENYKFREYKSDRENCK-GLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 360
           +EENYKFR+Y S  +    GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMPL
Sbjct: 301 YEENYKFRDYGSHGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMPL 360

Query: 361 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 420
           SR+ISP  S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDGVKVDV
Sbjct: 361 SRVISPKPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDGVKVDV 420

Query: 421 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 480
           IHLLEMLSEEFGGRIELAKAYY ALT SI+K+ +GNGVIASME CNDFMYLGTEAI LGR
Sbjct: 421 IHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEAIALGR 480

Query: 481 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 540
           VGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAIS
Sbjct: 481 VGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAIS 540

Query: 541 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 600
           GGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK WNLN
Sbjct: 541 GGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLKIWNLN 600

Query: 601 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 660
           +FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KGV  FA
Sbjct: 601 QFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVYLFA 660

Query: 661 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 720
           IYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYV FAPIGL NMLN GGAI
Sbjct: 661 IYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLNCGGAI 720

Query: 721 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 780
           QSLEI+ENEGLVRVGARGCGE+ VFAS+ PRSCKIDGEDV FEY DD+MVKIQVPWP SS
Sbjct: 721 QSLEIEENEGLVRVGARGCGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVPWPISS 780

Query: 781 RLSIIEYRF 784
           RLSIIEY+F
Sbjct: 781 RLSIIEYQF 782

BLAST of MC03g1020 vs. NCBI nr
Match: XP_022924071.1 (galactinol--sucrose galactosyltransferase-like [Cucurbita moschata])

HSP 1 Score: 1311 bits (3393), Expect = 0.0
Identity = 638/789 (80.86%), Postives = 698/789 (88.47%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLT N I D+V+ +L+A S   LSI LK S FVANGH P LTHVP  ITATPS LI
Sbjct: 40  MAPPSLTSNPI-DDVIPLLDAPSSD-LSIALKGSEFVANGH-PFLTHVPSNITATPSQLI 99

Query: 61  SETET----QLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
              +T     L  GCF+GFDA E  SCH+  IG LRGIRFSSIFRFKVWWTT WVG+CG 
Sbjct: 100 FSHKTTIPKSLLGGCFLGFDAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWVGSCGG 159

Query: 121 DVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASR 180
           D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPGGD  D VAMWVESGST V+AS+
Sbjct: 160 DIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPGGD--DNVAMWVESGSTTVQASQ 219

Query: 181 FRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNP 240
           FRSCLY+QVG+DPY+LV + M  VKL LGTFKLL++KTPP I+DKFGWCTWDAFYLKVNP
Sbjct: 220 FRSCLYMQVGKDPYTLVNEGMNVVKLHLGTFKLLDDKTPPRIVDKFGWCTWDAFYLKVNP 279

Query: 241 QGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIK 300
            G+ TGVKCLVDGGCPPGM+LIDDGWQSIAHDADSI D   QEAMDLT AGEQMPCRLIK
Sbjct: 280 HGVWTGVKCLVDGGCPPGMILIDDGWQSIAHDADSIADPH-QEAMDLTVAGEQMPCRLIK 339

Query: 301 FEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 360
           +EENYKFR+Y S  + +  GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMPL
Sbjct: 340 YEENYKFRDYGSRGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMPL 399

Query: 361 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 420
           SR+ISPN S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDGVKVDV
Sbjct: 400 SRVISPNPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDGVKVDV 459

Query: 421 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 480
           IHLLEMLSEEFGGRIELAKAYY ALT SI+K+ +GNGVIASME CNDFMYLGTEAI LGR
Sbjct: 460 IHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEAIALGR 519

Query: 481 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 540
           VGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAIS
Sbjct: 520 VGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAIS 579

Query: 541 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 600
           GGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK WNLN
Sbjct: 580 GGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLKIWNLN 639

Query: 601 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 660
           +FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KGV+ FA
Sbjct: 640 QFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVHLFA 699

Query: 661 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 720
           IYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYV FAPIGL NMLN GGAI
Sbjct: 700 IYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLNCGGAI 759

Query: 721 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 780
           QSLEI+ENEGLVRVGARG GE+ VFAS+ PRSCKIDGEDV FEY DD+MVKIQV WP SS
Sbjct: 760 QSLEIEENEGLVRVGARGYGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVTWPISS 819

Query: 781 RLSIIEYRF 784
           RLSIIEY+F
Sbjct: 820 RLSIIEYQF 821

BLAST of MC03g1020 vs. NCBI nr
Match: KAG7019802.1 (Galactinol--sucrose galactosyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1309 bits (3387), Expect = 0.0
Identity = 637/789 (80.74%), Postives = 698/789 (88.47%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLT N I D+V+ +L+A S   LSI LK S FVANGH P LTHVP  ITATPS LI
Sbjct: 1   MAPPSLTSNPI-DDVIPLLDAPSSD-LSIALKGSEFVANGH-PFLTHVPSNITATPSQLI 60

Query: 61  SETET----QLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
              +T     L  GCF+GFDA E  SCH+  IG LRGIRFSSIFRFKVWWTT WVG+CG 
Sbjct: 61  FCHKTTIPKSLLGGCFLGFDAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWVGSCGG 120

Query: 121 DVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASR 180
           D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPGGD  D VAMWVESGST V+AS+
Sbjct: 121 DIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPGGD--DNVAMWVESGSTTVQASQ 180

Query: 181 FRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNP 240
           FRSCLY+QVG+DPY+LV + M  VKL LGTFKLL++KTPP I+DKFGWCTWDAFYLKVNP
Sbjct: 181 FRSCLYMQVGKDPYTLVNEGMNVVKLHLGTFKLLDDKTPPPIVDKFGWCTWDAFYLKVNP 240

Query: 241 QGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIK 300
            G+ TGVKCLV+GGCPPGM+LIDDGWQSIAHDADSI D   QEAMDLT AGEQMPCRLIK
Sbjct: 241 HGVWTGVKCLVEGGCPPGMILIDDGWQSIAHDADSIADPH-QEAMDLTVAGEQMPCRLIK 300

Query: 301 FEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 360
           +EENYKFR+Y S  + +  GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMPL
Sbjct: 301 YEENYKFRDYGSRGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMPL 360

Query: 361 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 420
           SR+ISP  S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDGVKVDV
Sbjct: 361 SRVISPKPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDGVKVDV 420

Query: 421 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 480
           IHLLEMLSEEFGGRIELAKAYY ALT SI+K+ +GNGVIASME CNDFMYLGTEAI LGR
Sbjct: 421 IHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEAIALGR 480

Query: 481 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 540
           VGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAIS
Sbjct: 481 VGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAIS 540

Query: 541 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 600
           GGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK WNLN
Sbjct: 541 GGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLKIWNLN 600

Query: 601 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 660
           +FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KGV+ FA
Sbjct: 601 QFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVHLFA 660

Query: 661 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 720
           IYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYV FAPIGL NMLN GGAI
Sbjct: 661 IYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLNCGGAI 720

Query: 721 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 780
           QSLEI+ENEGLVRVGARG GE+ VFAS+ PRSCKIDGEDV FEY DD+MVKIQVPWP SS
Sbjct: 721 QSLEIEENEGLVRVGARGYGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVPWPISS 780

Query: 781 RLSIIEYRF 784
           RLSIIEY+F
Sbjct: 781 RLSIIEYQF 782

BLAST of MC03g1020 vs. ExPASy TrEMBL
Match: A0A6J1C5K5 (galactinol--sucrose galactosyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111008605 PE=3 SV=1)

HSP 1 Score: 1633 bits (4228), Expect = 0.0
Identity = 784/784 (100.00%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI
Sbjct: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60

Query: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120
           SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH
Sbjct: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120

Query: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180
           ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC
Sbjct: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180

Query: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240
           LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR
Sbjct: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240

Query: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300
           TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN
Sbjct: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300

Query: 301 YKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS 360
           YKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS
Sbjct: 301 YKFREYKSDRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS 360

Query: 361 PNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLE 420
           PNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLE
Sbjct: 361 PNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLE 420

Query: 421 MLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDF 480
           MLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDF
Sbjct: 421 MLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDF 480

Query: 481 WTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIY 540
           WTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIY
Sbjct: 481 WTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIY 540

Query: 541 ISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGA 600
           ISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGA
Sbjct: 541 ISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGA 600

Query: 601 LGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVR 660
           LGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVR
Sbjct: 601 LGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVR 660

Query: 661 QKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEI 720
           QKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEI
Sbjct: 661 QKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEI 720

Query: 721 DENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSSRLSII 780
           DENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSSRLSII
Sbjct: 721 DENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSSRLSII 780

Query: 781 EYRF 784
           EYRF
Sbjct: 781 EYRF 784

BLAST of MC03g1020 vs. ExPASy TrEMBL
Match: A0A6J1E8I6 (galactinol--sucrose galactosyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111431613 PE=3 SV=1)

HSP 1 Score: 1311 bits (3393), Expect = 0.0
Identity = 638/789 (80.86%), Postives = 698/789 (88.47%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLT N I D+V+ +L+A S   LSI LK S FVANGH P LTHVP  ITATPS LI
Sbjct: 40  MAPPSLTSNPI-DDVIPLLDAPSSD-LSIALKGSEFVANGH-PFLTHVPSNITATPSQLI 99

Query: 61  SETET----QLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
              +T     L  GCF+GFDA E  SCH+  IG LRGIRFSSIFRFKVWWTT WVG+CG 
Sbjct: 100 FSHKTTIPKSLLGGCFLGFDAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWVGSCGG 159

Query: 121 DVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASR 180
           D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPGGD  D VAMWVESGST V+AS+
Sbjct: 160 DIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPGGD--DNVAMWVESGSTTVQASQ 219

Query: 181 FRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNP 240
           FRSCLY+QVG+DPY+LV + M  VKL LGTFKLL++KTPP I+DKFGWCTWDAFYLKVNP
Sbjct: 220 FRSCLYMQVGKDPYTLVNEGMNVVKLHLGTFKLLDDKTPPRIVDKFGWCTWDAFYLKVNP 279

Query: 241 QGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIK 300
            G+ TGVKCLVDGGCPPGM+LIDDGWQSIAHDADSI D   QEAMDLT AGEQMPCRLIK
Sbjct: 280 HGVWTGVKCLVDGGCPPGMILIDDGWQSIAHDADSIADPH-QEAMDLTVAGEQMPCRLIK 339

Query: 301 FEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 360
           +EENYKFR+Y S  + +  GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMPL
Sbjct: 340 YEENYKFRDYGSRGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMPL 399

Query: 361 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 420
           SR+ISPN S+ LEMTMEDLAVDKIV++G+G VPPELAH+MY+GLHSHLQSAGIDGVKVDV
Sbjct: 400 SRVISPNPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHEMYDGLHSHLQSAGIDGVKVDV 459

Query: 421 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 480
           IHLLEMLSEEFGGRIELAKAYY ALT SI+K+ +GNGVIASME CNDFMYLGTEAI LGR
Sbjct: 460 IHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEAIALGR 519

Query: 481 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 540
           VGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAIS
Sbjct: 520 VGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAIS 579

Query: 541 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 600
           GGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK WNLN
Sbjct: 580 GGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLKIWNLN 639

Query: 601 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 660
           +FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KGV+ FA
Sbjct: 640 QFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVHLFA 699

Query: 661 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 720
           IYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYV FAPIGL NMLN GGAI
Sbjct: 700 IYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLNCGGAI 759

Query: 721 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 780
           QSLEI+ENEGLVRVGARG GE+ VFAS+ PRSCKIDGEDV FEY DD+MVKIQV WP SS
Sbjct: 760 QSLEIEENEGLVRVGARGYGEMRVFASKEPRSCKIDGEDVEFEY-DDKMVKIQVTWPISS 819

Query: 781 RLSIIEYRF 784
           RLSIIEY+F
Sbjct: 820 RLSIIEYQF 821

BLAST of MC03g1020 vs. ExPASy TrEMBL
Match: A0A6J1KNB0 (galactinol--sucrose galactosyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111495047 PE=3 SV=1)

HSP 1 Score: 1302 bits (3370), Expect = 0.0
Identity = 632/789 (80.10%), Postives = 695/789 (88.09%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLT N + D+V+ +L+A S   LSI LK S FVANGH P LTHVP  I ATPS  I
Sbjct: 1   MAPPSLTSNPV-DDVIPLLDAPSFD-LSIALKGSEFVANGH-PFLTHVPSNIKATPSQHI 60

Query: 61  SETETQ----LAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
              +T+    L  GCF+GFDA E  SCH+  IG LRGIRFSSIFRFKVWWTT WVG+CG 
Sbjct: 61  FSHKTKIPKSLLGGCFLGFDAAEPKSCHLVPIGNLRGIRFSSIFRFKVWWTTHWVGSCGR 120

Query: 121 DVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASR 180
           D+QHETQMMILD +DQGRPFVL LPILEGAFRCSLRPG D  DTVAMWVESGST V+AS+
Sbjct: 121 DIQHETQMMILDTNDQGRPFVLFLPILEGAFRCSLRPGDD--DTVAMWVESGSTTVQASQ 180

Query: 181 FRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNP 240
           FRSCLY+QVG+DPY+LV +AM  VKL LGTF+LL++KTPP I+DKFGWCTWDAFYLKVNP
Sbjct: 181 FRSCLYMQVGKDPYTLVNEAMNVVKLHLGTFRLLDDKTPPPIVDKFGWCTWDAFYLKVNP 240

Query: 241 QGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIK 300
            G+ TGVKCLVDGGCPPGM+LIDDGWQSIAHDADSI D   QEAMDLT AGEQMPCRLIK
Sbjct: 241 HGVMTGVKCLVDGGCPPGMILIDDGWQSIAHDADSIADPH-QEAMDLTVAGEQMPCRLIK 300

Query: 301 FEENYKFREYKS-DRENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPL 360
           +EENYKFR+Y S  + +  GLGAFV+DLKEEFR++E+VYVWHALCGYWGGIRPNV GMPL
Sbjct: 301 YEENYKFRDYGSRGKGSGVGLGAFVRDLKEEFRTIEHVYVWHALCGYWGGIRPNVPGMPL 360

Query: 361 SRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDV 420
           SR+ISP  S+ LEMTMEDLAVDKIV++G+G VPPELAHKMY+GLHSHLQSAGIDGVKVDV
Sbjct: 361 SRVISPKPSKGLEMTMEDLAVDKIVNNGVGFVPPELAHKMYDGLHSHLQSAGIDGVKVDV 420

Query: 421 IHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGR 480
           IHLLEMLSEEFGGRIELAKAYY ALT SI+K+ +GNGVIASME CNDFMYLGTEAI LGR
Sbjct: 421 IHLLEMLSEEFGGRIELAKAYYKALTASINKHLQGNGVIASMEQCNDFMYLGTEAIALGR 480

Query: 481 VGDDFWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAIS 540
           VGDDFWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAIS
Sbjct: 481 VGDDFWTVDASGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAIS 540

Query: 541 GGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLN 600
           GGPIY+SDSVG HNF LLK LVLPDGSILRCQ++ALP+RDCLFEDPL DGKT+LK WNLN
Sbjct: 541 GGPIYVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPSRDCLFEDPLQDGKTMLKIWNLN 600

Query: 601 KFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFA 660
           +FTGALGLFNCQGGGWCPKTRQNRR SEYARTLTCVAGPKDIEWNNGK PIS+KGV  FA
Sbjct: 601 QFTGALGLFNCQGGGWCPKTRQNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVYLFA 660

Query: 661 IYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAI 720
           IYM+++KKL LLK SENLE++IAPL+Y+LLVVSPVTVLSKPYV FAPIGL NMLN GGAI
Sbjct: 661 IYMIQEKKLRLLKTSENLEFTIAPLSYELLVVSPVTVLSKPYVEFAPIGLVNMLNCGGAI 720

Query: 721 QSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQMVKIQVPWPGSS 780
           QSLEI+ENEGLV+VG  GCGE+ VFAS+ PRSC+IDGED  FEY DD+MVKIQVPWP SS
Sbjct: 721 QSLEIEENEGLVKVGVGGCGEMRVFASKEPRSCEIDGEDAEFEY-DDKMVKIQVPWPISS 780

Query: 781 RLSIIEYRF 784
           RLSIIEY+F
Sbjct: 781 RLSIIEYQF 782

BLAST of MC03g1020 vs. ExPASy TrEMBL
Match: A0A0A0LWD8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G046280 PE=3 SV=1)

HSP 1 Score: 1299 bits (3362), Expect = 0.0
Identity = 624/786 (79.39%), Postives = 686/786 (87.28%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAP SLTK AI+     +    S   LSITLK S F+ANG+ P LTHVPP I +TPSP  
Sbjct: 1   MAPSSLTKTAISTIPTFLDTTPSLNNLSITLKASEFLANGY-PFLTHVPPNIISTPSPF- 60

Query: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120
            +T   L +GCF+GFDA E  S H+  IG L+GIRFSS+FRFKVWWTT WVGTCG D+QH
Sbjct: 61  -KTNNSL-LGCFIGFDATEPKSSHLVPIGNLKGIRFSSLFRFKVWWTTHWVGTCGRDIQH 120

Query: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180
           ETQMMILD +  GRP+VLLLPILEGAFRCSLR G + DD+VAMWVESGST V AS FRSC
Sbjct: 121 ETQMMILDTNHHGRPYVLLLPILEGAFRCSLRSGDENDDSVAMWVESGSTTVHASSFRSC 180

Query: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240
           LY+QVG+DPYSL+K+AMK VKL LGTFKLL EKTPP I+DKFGWCTWDAFYLKVNPQGI+
Sbjct: 181 LYMQVGDDPYSLLKEAMKVVKLHLGTFKLLHEKTPPPIVDKFGWCTWDAFYLKVNPQGIK 240

Query: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300
            GVKCLVDGGCPPGM+LIDDGWQSIAHDADS TD   QEAMDLT AGEQMPCRLIKFEEN
Sbjct: 241 IGVKCLVDGGCPPGMILIDDGWQSIAHDADSFTD-HHQEAMDLTAAGEQMPCRLIKFEEN 300

Query: 301 YKFREYKSDRENCK-GLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRII 360
           YKFR+Y SD +    GLGAFV+DLKEEFR++E VYVWHALCGYWGGIRPNV  MPLSR++
Sbjct: 301 YKFRDYGSDGKGVGVGLGAFVRDLKEEFRTIENVYVWHALCGYWGGIRPNVPRMPLSRVV 360

Query: 361 SPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLL 420
            P LSQ LE TMEDLAVDKIV++GIGLVPPELAH+MY+GLHSHLQSAGIDGVKVDVIHLL
Sbjct: 361 IPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHEMYDGLHSHLQSAGIDGVKVDVIHLL 420

Query: 421 EMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDD 480
           EM+SEEFGGRIELAKAYY ALT SI K+ +GNG IASMEHCNDFMYLGTEAI LGRVGDD
Sbjct: 421 EMISEEFGGRIELAKAYYKALTASIKKHLQGNGAIASMEHCNDFMYLGTEAIALGRVGDD 480

Query: 481 FWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPI 540
           FWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAISGGPI
Sbjct: 481 FWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAISGGPI 540

Query: 541 YISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTG 600
           Y+SDSVG HNFNLLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LK WNLNKFTG
Sbjct: 541 YVSDSVGKHNFNLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNLNKFTG 600

Query: 601 ALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMV 660
            LGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNGK PIS+KGVN FAIYM+
Sbjct: 601 TLGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGKNPISLKGVNLFAIYMI 660

Query: 661 RQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLE 720
           R KKL+LLK SENLE++IAPL Y+LLVVSP TVLSKP + FAPIGL NMLN GGAI+SLE
Sbjct: 661 RDKKLKLLKTSENLEFTIAPLEYELLVVSPATVLSKPNMEFAPIGLVNMLNCGGAIESLE 720

Query: 721 IDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDD-QMVKIQVPWPGSSRLS 780
           IDENEGLV+VG RGCGE+ VFAS  P +CK++GEDV FEY+DD +MVK+Q+PWP SS+LS
Sbjct: 721 IDENEGLVKVGVRGCGEMRVFASNEPINCKMEGEDVEFEYDDDDKMVKLQIPWPSSSKLS 780

Query: 781 IIEYRF 784
           IIEY+F
Sbjct: 781 IIEYQF 781

BLAST of MC03g1020 vs. ExPASy TrEMBL
Match: A0A5D3BJB8 (Galactinol--sucrose galactosyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003830 PE=3 SV=1)

HSP 1 Score: 1297 bits (3357), Expect = 0.0
Identity = 628/786 (79.90%), Postives = 687/786 (87.40%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MAPPSLTK +I      +    S   LSITLK S F+ANG+ P LTHVPP I +TPSP  
Sbjct: 1   MAPPSLTKTSITTIPTFLDTNPSPNNLSITLKASEFLANGY-PFLTHVPPNIISTPSPF- 60

Query: 61  SETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQH 120
            +T   L +GCF+GFDA E  S H+  IG L+GIRFSS+FRFKVWWTT WVG+CG D+QH
Sbjct: 61  -KTNNSL-LGCFIGFDATEPKSSHLVPIGNLKGIRFSSLFRFKVWWTTHWVGSCGRDIQH 120

Query: 121 ETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSC 180
           ETQMMILD + +GRP+VLLLPILEGAFRCSLR G + DD+VAMWVESGST V ASRFRSC
Sbjct: 121 ETQMMILDTNHRGRPYVLLLPILEGAFRCSLRSGDENDDSVAMWVESGSTSVHASRFRSC 180

Query: 181 LYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIR 240
           LY+QVG+DPYSLVK+AMK VKL LGTFKLL EKTPP I+DKFGWCTWDAFYLKVNPQGIR
Sbjct: 181 LYMQVGDDPYSLVKEAMKVVKLHLGTFKLLHEKTPPPIVDKFGWCTWDAFYLKVNPQGIR 240

Query: 241 TGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEEN 300
           TGVKCLVDGGCPPGM+LIDDGWQSIAHDA SITD    EAMDLT AGEQMPCRLIKFEEN
Sbjct: 241 TGVKCLVDGGCPPGMILIDDGWQSIAHDAHSITD-HHLEAMDLTAAGEQMPCRLIKFEEN 300

Query: 301 YKFREYKSDRENCK-GLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRII 360
           YKFR+Y SD +    GLGAFVKDLKEEFR++E VYVWHALCGYWGGIRPNV  MPLSR++
Sbjct: 301 YKFRDYGSDGKGFGVGLGAFVKDLKEEFRTIENVYVWHALCGYWGGIRPNVPRMPLSRVV 360

Query: 361 SPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLL 420
            P LSQ LE TMEDLAVDKIV++GIGLVPPELAH+MY+GLHSHLQSAGIDGVKVDVIHLL
Sbjct: 361 IPKLSQGLEKTMEDLAVDKIVNNGIGLVPPELAHEMYDGLHSHLQSAGIDGVKVDVIHLL 420

Query: 421 EMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDD 480
           EMLSEEFGGRIELAKAYY ALT SI K+ +GNG IASMEHCNDFMYLGTEAI LGRVGDD
Sbjct: 421 EMLSEEFGGRIELAKAYYKALTASIKKHLQGNGAIASMEHCNDFMYLGTEAIALGRVGDD 480

Query: 481 FWTKDLSGDPYWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPI 540
           FWT D SGDPYWLQGCHMVHCAYNSLWMGN+IHPDWDMFQS HPCAEFHAASRAISGGPI
Sbjct: 481 FWTVDPSGDPYWLQGCHMVHCAYNSLWMGNIIHPDWDMFQSTHPCAEFHAASRAISGGPI 540

Query: 541 YISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTG 600
           Y+SDSVG HNF LLK LVLPDGSILRCQ++ALPTRDCLFEDPLHDGKT+LK WNLNKFTG
Sbjct: 541 YVSDSVGKHNFKLLKRLVLPDGSILRCQHYALPTRDCLFEDPLHDGKTMLKIWNLNKFTG 600

Query: 601 ALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMV 660
            LGLFNCQGGGWCPKTR+NRR SEYARTLTCVAGPKDIEWNNG++ IS+KGVN FAIYMV
Sbjct: 601 TLGLFNCQGGGWCPKTRKNRRTSEYARTLTCVAGPKDIEWNNGRSLISLKGVNLFAIYMV 660

Query: 661 RQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLE 720
           R KKL+LLK SE+LE++IAPLNY+LLVVSPVTVLSKP + FAPIGL NMLN GGAI+SLE
Sbjct: 661 RDKKLKLLKTSESLEFTIAPLNYELLVVSPVTVLSKPNMEFAPIGLVNMLNCGGAIESLE 720

Query: 721 IDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDD-QMVKIQVPWPGSSRLS 780
           IDENEGLV  G RGCGE+ VFAS  P +CKI+GEDV FEY+DD +MVKIQ+PWP SS+LS
Sbjct: 721 IDENEGLVIAGVRGCGEMRVFASNEPINCKIEGEDVEFEYDDDDKMVKIQIPWPSSSKLS 780

Query: 781 IIEYRF 784
           IIEY+F
Sbjct: 781 IIEYQF 781

BLAST of MC03g1020 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 1016.5 bits (2627), Expect = 1.1e-296
Identity = 489/800 (61.12%), Postives = 604/800 (75.50%), Query Frame = 0

Query: 1   MAPPSLTKNAINDEVVGVLNASSRPALSITLKDSNFVANGHHPILTHVPPYITATPSPLI 60
           MA P LTK   +D  +  ++ + +      L+DS  +ANG   +LT VP  +T T SP +
Sbjct: 1   MASPCLTK---SDSGINGVDFTEK----FRLEDSTLLANG-QVVLTDVPVNVTLTSSPYL 60

Query: 61  SETE---TQLAVGCFVGFDAD-ESNSCHVASIGRLRGIRFSSIFRFKVWWTTQWVGTCGS 120
            + +     ++ G F+GF+ D E  S HVASIG+L+ IRF SIFRFKVWWTT WVG+ G 
Sbjct: 61  VDKDGVPLDVSAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGR 120

Query: 121 DVQHETQMMILDR--------SDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESG 180
           D+++ETQ++ILD+        S  GRP+VLLLP+LEG+FR S + G   DD VA+ VESG
Sbjct: 121 DIENETQIIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSG--EDDDVAVCVESG 180

Query: 181 STCVRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWD 240
           ST V  S FR  +Y+  G+DP+ LVKDAMK +++ + TFKLLEEK+PPGI+DKFGWCTWD
Sbjct: 181 STEVTGSEFRQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWD 240

Query: 241 AFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGE 300
           AFYL VNP G+  GVKCLVDGGCPPG+VLIDDGWQSI HD+D I    + E M++T AGE
Sbjct: 241 AFYLTVNPDGVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGI----DVEGMNITVAGE 300

Query: 301 QMPCRLIKFEENYKFREYKSDR-ENCKGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIR 360
           QMPCRL+KFEEN+KF++Y S + +N  G+ AFV+DLK+EF +V+Y+YVWHALCGYWGG+R
Sbjct: 301 QMPCRLLKFEENHKFKDYVSPKDQNDVGMKAFVRDLKDEFSTVDYIYVWHALCGYWGGLR 360

Query: 361 PNVLGMPLSRIISPNLSQELEMTMEDLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAG 420
           P    +P S II P LS  L++TMEDLAVDKI+ +GIG   P+LA + YEGLHSHLQ+AG
Sbjct: 361 PEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLHSHLQNAG 420

Query: 421 IDGVKVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLG 480
           IDGVKVDVIH+LEML +++GGR++LAKAY+ ALT S++K+F GNGVIASMEHCNDFM+LG
Sbjct: 421 IDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHCNDFMFLG 480

Query: 481 TEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPC 540
           TEAI+LGRVGDDFW  D SGDP   +WLQGCHMVHCAYNSLWMGN I PDWDMFQS HPC
Sbjct: 481 TEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDMFQSTHPC 540

Query: 541 AEFHAASRAISGGPIYISDSVGSHNFNLLKSLVLPDGSILRCQYHALPTRDCLFEDPLHD 600
           AEFHAASRAISGGPIYISD VG H+F+LLK LVLP+GSILRC+Y+ALPTRD LFEDPLHD
Sbjct: 541 AEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRLFEDPLHD 600

Query: 601 GKTILKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEYARTLTCVAGPKDIEWNNGKT 660
           GKT+LK WNLNK+TG +G FNCQGGGWC +TR+N+  SE   TLT    PKD+EWN+G +
Sbjct: 601 GKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDVEWNSGSS 660

Query: 661 PISVKGVNFFAIYMVRQKKLELLKASENLEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIG 720
           PIS+  V  FA+++ + KKL L   +++LE ++ P  ++L+ VSPV  +    V FAPIG
Sbjct: 661 PISIANVEEFALFLSQSKKLLLSGLNDDLELTLEPFKFELITVSPVVTIEGNSVRFAPIG 720

Query: 721 LANMLNPGGAIQSLEIDENEGLVRVGARGCGEILVFASEGPRSCKIDGEDVGFEYEDDQM 780
           L NMLN  GAI+SL    N+  V VG  G GE  V+AS+ P SC IDGE V F YE D M
Sbjct: 721 LVNMLNTSGAIRSLVY--NDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFGYE-DSM 780

Query: 781 VKIQVPWPGSSRLSIIEYRF 785
           V +QVPW G   LS I+Y F
Sbjct: 781 VMVQVPWSGPDGLSSIQYLF 783

BLAST of MC03g1020 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 668.3 bits (1723), Expect = 7.5e-192
Identity = 362/872 (41.51%), Postives = 499/872 (57.22%), Query Frame = 0

Query: 1   MAPPSLTKNAIND--EVVGVLNASSRPAL---SITLKDSNFVANGHHPILTHVPPYITAT 60
           MAP   + ++IND  E   +    ++P L   S  L + +  A    PIL  VP  +T T
Sbjct: 1   MAPLHESLSSINDVIESKPLFVPITKPILQPNSFNLSEGSLCAKDSTPILFDVPQNVTFT 60

Query: 61  -----------PSPLISETETQLAVGCFVGFDADESNSCHVASIGRLRGIRFSSIFRFKV 120
                      P P++   +     G F+GF  +  +     S+GR     F S+FRFK+
Sbjct: 61  PFSSHSISTDAPLPILLRVQANAHKGGFLGFTKESPSDRLTNSLGRFEDREFLSLFRFKM 120

Query: 121 WWTTQWVGTCGSDVQHETQMMILDRSDQGRPFVLLLPILEGAFRCSLRPGGDGDDTVAMW 180
           WW+T W+G  GSD+Q ETQ ++L +  +   +V ++P +EGAFR SL PG  G+  V + 
Sbjct: 121 WWSTAWIGKSGSDLQAETQWVML-KIPEIDSYVAIIPTIEGAFRASLTPGEKGN--VLIC 180

Query: 181 VESGSTCVRASRFRSCLYIQVGEDPYSLVKDAMKAVKLSLGTFKLLEEKTPPGIMDKFGW 240
            ESGST V+ S F+S  YI + ++PY+L+K+A  A+++ + TFKLLEEK  P I+DKFGW
Sbjct: 181 AESGSTKVKESSFKSIAYIHICDNPYNLMKEAFSALRVHMNTFKLLEEKKLPKIVDKFGW 240

Query: 241 CTWDAFYLKVNPQGIRTGVKCLVDGGCPPGMVLIDDGWQSIAHDADSITDGREQEAMDLT 300
           CTWDA YL V+P  I TGVK   DGG  P  V+IDDGWQSI  D D +    +++A +L 
Sbjct: 241 CTWDACYLTVDPATIWTGVKEFEDGGVCPKFVIIDDGWQSINFDGDEL----DKDAENLV 300

Query: 301 TAGEQMPCRLIKFEENYKFREYK------------------------------------- 360
             GEQM  RL  F+E  KFR YK                                     
Sbjct: 301 LGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPLKPKMLIYKATERIQAIILRRKL 360

Query: 361 -------------------------------------SDRENCKGLGAFVKDLKEEFRSV 420
                                                SD  +  G+ AF KDL+  F+S+
Sbjct: 361 VKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLGSDDVSGSGMAAFTKDLRLRFKSL 420

Query: 421 EYVYVWHALCGYWGGIRPNVLGMPLSRIISP-NLSQELEMTMEDLAVDKIVSSGIGLVPP 480
           + +YVWHALCG W G+RP  + M L   ++P  LS  L  TM DLAVDK+V +GIGLV P
Sbjct: 421 DDIYVWHALCGAWNGVRPETM-MDLKAKVAPFELSPSLGATMADLAVDKVVEAGIGLVHP 480

Query: 481 ELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFGGRIELAKAYYTALTMSIHKYFK 540
             AH+ Y+ +HS+L S G+ G K+DV   LE L+EE GGR+ELAKAYY  LT S+ K F 
Sbjct: 481 SKAHEFYDSMHSYLASVGVTGAKIDVFQTLESLAEEHGGRVELAKAYYDGLTESMIKNFN 540

Query: 541 GNGVIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSGDP---YWLQGCHMVHCAYNSLW 600
           G  VIASM+ CN+F +L T+ I++GRVGDDFW +D  GDP   YWLQG HM+HC+YNS+W
Sbjct: 541 GTDVIASMQQCNEFFFLATKQISIGRVGDDFWWQDPYGDPQGVYWLQGVHMIHCSYNSIW 600

Query: 601 MGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDSVG--SHNFNLLKSLVLPDGSIL 660
           MG MI PDWDMFQS+H CAE+HAASRAI GGP+Y+SD +G  SHNF+L+K L   DG+I 
Sbjct: 601 MGQMIQPDWDMFQSDHVCAEYHAASRAICGGPVYLSDHLGKASHNFDLIKKLAFFDGTIP 660

Query: 661 RCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGALGLFNCQGGGWCPKTRQNRRASEY 720
           RC ++ALPTRD LF++PL D ++ILK +N NKF G +G FNCQG GW P+  + +   E 
Sbjct: 661 RCVHYALPTRDSLFKNPLFDKESILKIFNFNKFGGVIGTFNCQGAGWSPEEHRFKGYKEC 720

Query: 721 ARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMV-RQKKLELL---KASENLEYSIAPL 772
             T++      DIEW+        + V +   Y+V +Q+  E+L     SE ++ ++ P 
Sbjct: 721 YTTVSGTVHVSDIEWDQNPEAAGSQ-VTYTGDYLVYKQQSEEILFMNSKSEAMKITLEPS 780

BLAST of MC03g1020 vs. TAIR 10
Match: AT5G20250.1 (Raffinose synthase family protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-156
Identity = 294/748 (39.30%), Postives = 423/748 (56.55%), Query Frame = 0

Query: 28  SITLKDSNFVANGHHPILTHVPPYITATPSPLISETETQLAVGCFVGFDADESNSCHVAS 87
           ++ + D N +   +  ILT VP  +  T     S +E     G FVG   ++  S H+  
Sbjct: 6   AVRISDGNLIIK-NRTILTGVPDNVITT-----SASEAGPVEGVFVGAVFNKEESKHIVP 65

Query: 88  IGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMMILDRSD--------------QG 147
           IG LR  RF S FRFK+WW  Q +G  G D+ +ETQ ++++ +D                
Sbjct: 66  IGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQ 125

Query: 148 RPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSCLYIQVGEDPYSLV 207
           + + + LP++EG+FR  L+  G+ +D V + +ESG    + S F   LYI  G DP+  +
Sbjct: 126 KVYTVFLPLIEGSFRSCLQ--GNVNDEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTI 185

Query: 208 KDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIRTGVKCLVDGGCPP 267
            DA++ VKL L +F+   EK  PGI+D FGWCTWDAFY +V  +G+  G+K L  GG PP
Sbjct: 186 TDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPP 245

Query: 268 GMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEENYKFREYKSDRENC 327
             V+IDDGWQS+  DA ++  G E++        E    RL   +EN KF+  K D  N 
Sbjct: 246 KFVIIDDGWQSVERDA-TVEAGDEKK--------ESPIFRLTGIKENEKFK--KKDDPNV 305

Query: 328 KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS-PNLSQELEMTME 387
            G+   VK  KE+   ++YVYVWHA+ GYWGG+RP   G     ++  PN+S+ +     
Sbjct: 306 -GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRP---GEEYGSVMKYPNMSKGVVENDP 365

Query: 388 DLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFGGRIEL 447
               D +   G+GLV P+  +K Y  LHS+L  AG+DGVKVDV  +LE L    GGR+EL
Sbjct: 366 TWKTDVMTLQGLGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVEL 425

Query: 448 AKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSGDPYWL 507
            + ++ AL  S+ K F  NG IA M H  D +Y   +A  + R  DDF+ +D        
Sbjct: 426 TRQFHQALDSSVAKNFPDNGCIACMSHNTDALYCSKQAAVI-RASDDFYPRDPVS----- 485

Query: 508 QGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDSVGSHNFNL 567
              H+   AYNS+++G  + PDWDMF S HP AE+HA++RAISGGP+Y+SDS G HNF L
Sbjct: 486 HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFEL 545

Query: 568 LKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGALGLFNCQGGGWC 627
           L+ LVLPDGSILR +    PTRDCLF DP  DG ++LK WN+NK+TG LG++NCQG  W 
Sbjct: 546 LRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWS 605

Query: 628 PKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVRQKKLELLKASEN 687
              R+N        +LT     +D+   +  +          A+Y   + +L ++  + +
Sbjct: 606 STERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVYSQSRGELIVMPYNVS 665

Query: 688 LEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEIDENEGLVRVGAR 747
           L  S+    +++  VSP++ L    V FAPIGL NM N GGAI+ L  +  +  V +  +
Sbjct: 666 LPVSLKIREHEIFTVSPISHLVDG-VSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVK 722

Query: 748 GCGEILVFASEGPRSCKIDGEDVGFEYE 761
           GCG+   ++S  P+ C ++  ++ FEY+
Sbjct: 726 GCGKFGSYSSVKPKRCVVESNEIAFEYD 722

BLAST of MC03g1020 vs. TAIR 10
Match: AT5G20250.2 (Raffinose synthase family protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-156
Identity = 294/748 (39.30%), Postives = 423/748 (56.55%), Query Frame = 0

Query: 28  SITLKDSNFVANGHHPILTHVPPYITATPSPLISETETQLAVGCFVGFDADESNSCHVAS 87
           ++ + D N +   +  ILT VP  +  T     S +E     G FVG   ++  S H+  
Sbjct: 6   AVRISDGNLIIK-NRTILTGVPDNVITT-----SASEAGPVEGVFVGAVFNKEESKHIVP 65

Query: 88  IGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMMILDRSD--------------QG 147
           IG LR  RF S FRFK+WW  Q +G  G D+ +ETQ ++++ +D                
Sbjct: 66  IGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQ 125

Query: 148 RPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSCLYIQVGEDPYSLV 207
           + + + LP++EG+FR  L+  G+ +D V + +ESG    + S F   LYI  G DP+  +
Sbjct: 126 KVYTVFLPLIEGSFRSCLQ--GNVNDEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTI 185

Query: 208 KDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIRTGVKCLVDGGCPP 267
            DA++ VKL L +F+   EK  PGI+D FGWCTWDAFY +V  +G+  G+K L  GG PP
Sbjct: 186 TDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPP 245

Query: 268 GMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEENYKFREYKSDRENC 327
             V+IDDGWQS+  DA ++  G E++        E    RL   +EN KF+  K D  N 
Sbjct: 246 KFVIIDDGWQSVERDA-TVEAGDEKK--------ESPIFRLTGIKENEKFK--KKDDPNV 305

Query: 328 KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS-PNLSQELEMTME 387
            G+   VK  KE+   ++YVYVWHA+ GYWGG+RP   G     ++  PN+S+ +     
Sbjct: 306 -GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRP---GEEYGSVMKYPNMSKGVVENDP 365

Query: 388 DLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFGGRIEL 447
               D +   G+GLV P+  +K Y  LHS+L  AG+DGVKVDV  +LE L    GGR+EL
Sbjct: 366 TWKTDVMTLQGLGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVEL 425

Query: 448 AKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSGDPYWL 507
            + ++ AL  S+ K F  NG IA M H  D +Y   +A  + R  DDF+ +D        
Sbjct: 426 TRQFHQALDSSVAKNFPDNGCIACMSHNTDALYCSKQAAVI-RASDDFYPRDPVS----- 485

Query: 508 QGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDSVGSHNFNL 567
              H+   AYNS+++G  + PDWDMF S HP AE+HA++RAISGGP+Y+SDS G HNF L
Sbjct: 486 HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFEL 545

Query: 568 LKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGALGLFNCQGGGWC 627
           L+ LVLPDGSILR +    PTRDCLF DP  DG ++LK WN+NK+TG LG++NCQG  W 
Sbjct: 546 LRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWS 605

Query: 628 PKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVRQKKLELLKASEN 687
              R+N        +LT     +D+   +  +          A+Y   + +L ++  + +
Sbjct: 606 STERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVYSQSRGELIVMPYNVS 665

Query: 688 LEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEIDENEGLVRVGAR 747
           L  S+    +++  VSP++ L    V FAPIGL NM N GGAI+ L  +  +  V +  +
Sbjct: 666 LPVSLKIREHEIFTVSPISHLVDG-VSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVK 722

Query: 748 GCGEILVFASEGPRSCKIDGEDVGFEYE 761
           GCG+   ++S  P+ C ++  ++ FEY+
Sbjct: 726 GCGKFGSYSSVKPKRCVVESNEIAFEYD 722

BLAST of MC03g1020 vs. TAIR 10
Match: AT5G20250.3 (Raffinose synthase family protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-156
Identity = 294/748 (39.30%), Postives = 423/748 (56.55%), Query Frame = 0

Query: 28  SITLKDSNFVANGHHPILTHVPPYITATPSPLISETETQLAVGCFVGFDADESNSCHVAS 87
           ++ + D N +   +  ILT VP  +  T     S +E     G FVG   ++  S H+  
Sbjct: 6   AVRISDGNLIIK-NRTILTGVPDNVITT-----SASEAGPVEGVFVGAVFNKEESKHIVP 65

Query: 88  IGRLRGIRFSSIFRFKVWWTTQWVGTCGSDVQHETQMMILDRSD--------------QG 147
           IG LR  RF S FRFK+WW  Q +G  G D+ +ETQ ++++ +D                
Sbjct: 66  IGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESDGANGVECNQ 125

Query: 148 RPFVLLLPILEGAFRCSLRPGGDGDDTVAMWVESGSTCVRASRFRSCLYIQVGEDPYSLV 207
           + + + LP++EG+FR  L+  G+ +D V + +ESG    + S F   LYI  G DP+  +
Sbjct: 126 KVYTVFLPLIEGSFRSCLQ--GNVNDEVELCLESGDVDTKRSSFTHSLYIHAGTDPFQTI 185

Query: 208 KDAMKAVKLSLGTFKLLEEKTPPGIMDKFGWCTWDAFYLKVNPQGIRTGVKCLVDGGCPP 267
            DA++ VKL L +F+   EK  PGI+D FGWCTWDAFY +V  +G+  G+K L  GG PP
Sbjct: 186 TDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSLAAGGTPP 245

Query: 268 GMVLIDDGWQSIAHDADSITDGREQEAMDLTTAGEQMPCRLIKFEENYKFREYKSDRENC 327
             V+IDDGWQS+  DA ++  G E++        E    RL   +EN KF+  K D  N 
Sbjct: 246 KFVIIDDGWQSVERDA-TVEAGDEKK--------ESPIFRLTGIKENEKFK--KKDDPNV 305

Query: 328 KGLGAFVKDLKEEFRSVEYVYVWHALCGYWGGIRPNVLGMPLSRIIS-PNLSQELEMTME 387
            G+   VK  KE+   ++YVYVWHA+ GYWGG+RP   G     ++  PN+S+ +     
Sbjct: 306 -GIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRP---GEEYGSVMKYPNMSKGVVENDP 365

Query: 388 DLAVDKIVSSGIGLVPPELAHKMYEGLHSHLQSAGIDGVKVDVIHLLEMLSEEFGGRIEL 447
               D +   G+GLV P+  +K Y  LHS+L  AG+DGVKVDV  +LE L    GGR+EL
Sbjct: 366 TWKTDVMTLQGLGLVSPKKVYKFYNELHSYLADAGVDGVKVDVQCVLETLGGGLGGRVEL 425

Query: 448 AKAYYTALTMSIHKYFKGNGVIASMEHCNDFMYLGTEAITLGRVGDDFWTKDLSGDPYWL 507
            + ++ AL  S+ K F  NG IA M H  D +Y   +A  + R  DDF+ +D        
Sbjct: 426 TRQFHQALDSSVAKNFPDNGCIACMSHNTDALYCSKQAAVI-RASDDFYPRDPVS----- 485

Query: 508 QGCHMVHCAYNSLWMGNMIHPDWDMFQSNHPCAEFHAASRAISGGPIYISDSVGSHNFNL 567
              H+   AYNS+++G  + PDWDMF S HP AE+HA++RAISGGP+Y+SDS G HNF L
Sbjct: 486 HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEYHASARAISGGPLYVSDSPGKHNFEL 545

Query: 568 LKSLVLPDGSILRCQYHALPTRDCLFEDPLHDGKTILKFWNLNKFTGALGLFNCQGGGWC 627
           L+ LVLPDGSILR +    PTRDCLF DP  DG ++LK WN+NK+TG LG++NCQG  W 
Sbjct: 546 LRKLVLPDGSILRARLPGRPTRDCLFADPARDGVSLLKIWNMNKYTGVLGVYNCQGAAWS 605

Query: 628 PKTRQNRRASEYARTLTCVAGPKDIEWNNGKTPISVKGVNFFAIYMVRQKKLELLKASEN 687
              R+N        +LT     +D+   +  +          A+Y   + +L ++  + +
Sbjct: 606 STERKNIFHQTKTDSLTGSIRGRDVHSISEASTDPTTWNGDCAVYSQSRGELIVMPYNVS 665

Query: 688 LEYSIAPLNYQLLVVSPVTVLSKPYVGFAPIGLANMLNPGGAIQSLEIDENEGLVRVGAR 747
           L  S+    +++  VSP++ L    V FAPIGL NM N GGAI+ L  +  +  V +  +
Sbjct: 666 LPVSLKIREHEIFTVSPISHLVDG-VSFAPIGLVNMYNSGGAIEGLRYEAEKMKVVMEVK 722

Query: 748 GCGEILVFASEGPRSCKIDGEDVGFEYE 761
           GCG+   ++S  P+ C ++  ++ FEY+
Sbjct: 726 GCGKFGSYSSVKPKRCVVESNEIAFEYD 722