MC03g0890 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0890
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Lactoylglutathione lyase
Location	MC03: 15526416 .. 15527937 (-)
RNA-Seq Expression	MC03g0890
Synteny	MC03g0890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGGCTCCTGATGCAGATGCTCAGTTGTTGGAATGGCCCAAGAAAGACAACCGCCGCTTGCTTCATGCTGTCTATTGTGTTGGTGATCTCGATCGCACCATCAAGTAAATTTTCTTCATTCTTACTCTGGATCTCTCTCTTCTCTGTCTTTCTCTTCATGGCCATGTATTTTCCTTCTTCAGGTTCTACACTGAATGCTTTGGGATGAAACTCCTGCGAATTAGAGATGTCCCAGAAGAGAAGTATTCCAATGCATTCATGGGGTTTGGCCCCGAGCAATCTTCGTTCGTCGTCGAGCTGATTTACAGTACGTGGCTTTAACAACTTCTCTGAATCAAGTCATAATCTTCTTCCCTCTTCAATCCAAACTTGTGTTGAAATCATACAGATCATGGAGTAACTTCATATGATATTGGTACTGGATTTGGACACTTTGCCATTGCTACTCAGGATGTGAGTAAAACTAATCAATCAAGTGTCATTCTTCATGACTTTGCCTTGTTCTGCAACTTGAGATTTAGAACATTAACGTTGACCTTCTGTTCATTTCCAAGGTTTACAAAATGGTTGAAAATATCACGGCCAAGAGTGCTGATAGTATCATCAGAGAACCTTTTGAACTGAAAGGCAGCTCCTTTGTGCTGGCCTATGTGAAAGATCCTGATGGTTACATCTTTGAGCTGATCCAAAGAGCTCCCACTCCTCAGCCACTCTGCCATGTAATGCTTCGTGTTGCCGATTTACAACGCTCTATCAACTTCTATGAGAAGGTAAAAAGAATTTCTCCCCACAACTGTTGAAAAAAGTCAAGAGAGACGTTTTTCTGCAGTGCCACTAAAAATTCTCATCCTGAGTTATAATTCTGCATGATTTTGTCTCTGTACAGGCATTGGGAATGAGGTCACTTAATAAGGTTGAGAGTGTTGAACACAAGGTGAACTTTAATATTGACCAATTACAGAGTTTCTATTCATCTTCCAAAAGTAAAGCCTGATGAAATGCTATAATTCTCAGTATGCCATAGCCATTATGGGGTATGCAGATGAGTCAGAGACAGTGGCGCTGGAGTTGACTTACAATCATGGTGTGACAGAACATTCCAAGGGAAATGGATATTCCCAGGTCAACACTTATGTTTTTGTTGGAGTTGTCCTAGATTAAAGTACCATTTTCCCATGTTTATGGTAACTTCTAGTTCTGGTTTCAGGTTGCTATTGGCACAGATGATGTATACAAGAGTGCTGAGGTTGTCAATCTGATTACCAAAGAGCTTGGAGGAAAGATAACCCAGCAGCCCAGTTTGATTCATCCAATCAATGCGAAGATTACCGAGTTTCTCGATCCCGATGGCTGGAAAATTGTGAGTTATCTTTATAAAATCAAATCCTTGCAAATGACAAGTGGATTTTTGTTAAACTATACGCTTGATCAGCTACGAAACTATGTTCAAGTAGTGCTTCAATCCAAACCTTCTCTGTTTTAGGTTCTTGTTGACAATGAAGACTATCTGAAGGAGCTGCAG

mRNA sequence

CTGGCTCCTGATGCAGATGCTCAGTTGTTGGAATGGCCCAAGAAAGACAACCGCCGCTTGCTTCATGCTGTCTATTGTGTTGGTGATCTCGATCGCACCATCAAGTTCTACACTGAATGCTTTGGGATGAAACTCCTGCGAATTAGAGATGTCCCAGAAGAGAAGTATTCCAATGCATTCATGGGGTTTGGCCCCGAGCAATCTTCGTTCGTCGTCGAGCTGATTTACAATCATGGAGTAACTTCATATGATATTGGTACTGGATTTGGACACTTTGCCATTGCTACTCAGGATAACATTAACGTTGACCTTCTGTTCATTTCCAAGGTTTACAAAATGGTTGAAAATATCACGGCCAAGAGTGCTGATAGTATCATCAGAGAACCTTTTGAACTGAAAGGCAGCTCCTTTGTGCTGGCCTATGTGAAAGATCCTGATGGTTACATCTTTGAGCTGATCCAAAGAGCTCCCACTCCTCAGCCACTCTGCCATGTAATGCTTCGTGTTGCCGATTTACAACGCTCTATCAACTTCTATGAGAAGGCATTGGGAATGAGGTCACTTAATAAGGTTGAGAGTGTTGAACACAAGTATGCCATAGCCATTATGGGGTATGCAGATGAGTCAGAGACAGTGGCGCTGGAGTTGACTTACAATCATGGTGTGACAGAACATTCCAAGGGAAATGGATATTCCCAGGTTGCTATTGGCACAGATGATGTATACAAGAGTGCTGAGGTTGTCAATCTGATTACCAAAGAGCTTGGAGGAAAGATAACCCAGCAGCCCAGTTTGATTCATCCAATCAATGCGAAGATTACCGAGTTTCTCGATCCCGATGGCTGGAAAATTGTTCTTGTTGACAATGAAGACTATCTGAAGGAGCTGCAG

Coding sequence (CDS)

CTGGCTCCTGATGCAGATGCTCAGTTGTTGGAATGGCCCAAGAAAGACAACCGCCGCTTGCTTCATGCTGTCTATTGTGTTGGTGATCTCGATCGCACCATCAAGTTCTACACTGAATGCTTTGGGATGAAACTCCTGCGAATTAGAGATGTCCCAGAAGAGAAGTATTCCAATGCATTCATGGGGTTTGGCCCCGAGCAATCTTCGTTCGTCGTCGAGCTGATTTACAATCATGGAGTAACTTCATATGATATTGGTACTGGATTTGGACACTTTGCCATTGCTACTCAGGATAACATTAACGTTGACCTTCTGTTCATTTCCAAGGTTTACAAAATGGTTGAAAATATCACGGCCAAGAGTGCTGATAGTATCATCAGAGAACCTTTTGAACTGAAAGGCAGCTCCTTTGTGCTGGCCTATGTGAAAGATCCTGATGGTTACATCTTTGAGCTGATCCAAAGAGCTCCCACTCCTCAGCCACTCTGCCATGTAATGCTTCGTGTTGCCGATTTACAACGCTCTATCAACTTCTATGAGAAGGCATTGGGAATGAGGTCACTTAATAAGGTTGAGAGTGTTGAACACAAGTATGCCATAGCCATTATGGGGTATGCAGATGAGTCAGAGACAGTGGCGCTGGAGTTGACTTACAATCATGGTGTGACAGAACATTCCAAGGGAAATGGATATTCCCAGGTTGCTATTGGCACAGATGATGTATACAAGAGTGCTGAGGTTGTCAATCTGATTACCAAAGAGCTTGGAGGAAAGATAACCCAGCAGCCCAGTTTGATTCATCCAATCAATGCGAAGATTACCGAGTTTCTCGATCCCGATGGCTGGAAAATTGTTCTTGTTGACAATGAAGACTATCTGAAGGAGCTGCAG

Protein sequence

LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ

Homology

[Close]

BLAST of MC03g0890 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 403.3 bits (1035), Expect = 2.4e-111
Identity = 192/289 (66.44%), Postives = 228/289 (78.89%), Query Frame = 0


Query: 9   LLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPEQS 68
           +LEWPKKD +RLLHAVY VGDLDRTIK YTECFGMKLLR RDVPEEKY+NAF+GFGPE +
Sbjct: 14  VLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDT 73

Query: 69  SFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSIIRE 128
           +F +EL YN+GV  YDIG GFGHFAIAT+D           VYK+ E I +     I RE
Sbjct: 74  NFALELTYNYGVDKYDIGAGFGHFAIATED-----------VYKLAEKIKSSCCCKITRE 133

Query: 129 PFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMRSL 188
           P  +KG S V+A+ +DPDGY+FELIQR PTP+PLC VMLRV DL RSI FYEKALGM+ L
Sbjct: 134 PGPVKGGSTVIAFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLL 193

Query: 189 NKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAEVV 248
            K +  ++KY IA++GYADE +T  +ELTYN+GVTE++KGN Y+QVAIGT+DVYKSAE V
Sbjct: 194 RKKDVPDYKYTIAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAV 253

Query: 249 NLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
            L+TKELGGKI +QP  +  +N KI  FLDPDGWK+VLVDN D+LKELQ
Sbjct: 254 ELVTKELGGKILRQPGPLPGLNTKIASFLDPDGWKVVLVDNADFLKELQ 291

[Close]

BLAST of MC03g0890 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 5.3e-111
Identity = 192/291 (65.98%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 7   AQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPE 66
           + LLEWPKKDNRR LH VY VGDLDRTI+FYTE FGMKLLR RD+PEEKYSNAF+GFGPE
Sbjct: 5   SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 64

Query: 67  QSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSII 126
            S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V K+VEN+ AK   ++ 
Sbjct: 65  TSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKLVENVRAKGG-NVT 124

Query: 127 REPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMR 186
           REP  +KG   V+A+VKDPDGY FELIQR PTP+P C VMLRV DL R+I FYEKALGMR
Sbjct: 125 REPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMR 184

Query: 187 SLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAE 246
            L K+E  E+KY I +MGYA+E E++ LELTYN+ VTE++KGN Y+Q+AIGTDDVYKS E
Sbjct: 185 LLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 244

Query: 247 VVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           V+ ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDN+D+LKEL+
Sbjct: 245 VIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MC03g0890 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 8.5e-109
Identity = 192/292 (65.75%), Postives = 231/292 (79.11%), Query Frame = 0


Query: 6   DAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGP 65
           +A L+EWPKKD RR LH VY VGDLDRTI+FYTECFGMK+LR RDVPEEKYSNAF+GFGP
Sbjct: 4   NADLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGP 63

Query: 66  EQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSI 125
           E S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V KMVE + AK   ++
Sbjct: 64  ETSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKMVEAVRAKGG-NV 123

Query: 126 IREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGM 185
            REP  +KG   V+A+VKDPDGY FELIQR PTP+PLC VMLRV DL R++ F EKALGM
Sbjct: 124 TREPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGM 183

Query: 186 RSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSA 245
           R L ++E  E+   I +MGYA+E E++ LELTYN+GVTE++KGN Y+Q+AIGTDDVYKSA
Sbjct: 184 RLLRRIERPEYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSA 243

Query: 246 EVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           EVV ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDNED+LKEL+
Sbjct: 244 EVVKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKQVLVDNEDFLKELE 282

[Close]

BLAST of MC03g0890 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.3e-96
Identity = 172/289 (59.52%), Postives = 208/289 (71.97%), Query Frame = 0


Query: 9   LLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPEQS 68
           LL W K D RR+LH VY VGD+DRTIKFYTEC GMKLLR RD+PEEKY+NAF+G+GPE S
Sbjct: 78  LLTWVKNDKRRMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEDS 137

Query: 69  SFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSIIRE 128
            FV+EL YN+GV  YDIG GFGHF IA  D           V K VE + AK    + RE
Sbjct: 138 HFVIELTYNYGVDKYDIGAGFGHFGIAVDD-----------VAKTVELVKAKGG-KVSRE 197

Query: 129 PFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMRSL 188
           P  +KG   V+A+++DPDGY FEL++R PTP+PLC VMLRV DL R+I FYEKA GM  L
Sbjct: 198 PGPVKGGKTVIAFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAIKFYEKAFGMELL 257

Query: 189 NKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAEVV 248
              ++ E+KY IA+MGY  E +   LELTYN+GVTE+ KGN Y+Q+AIGTDDVYK+AE +
Sbjct: 258 RTRDNPEYKYTIAMMGYGPEDKFPVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEAI 317

Query: 249 NLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
            L     GGKIT++P  +  I+ KIT  LDPDGWK V VDN D+LKEL+
Sbjct: 318 KL----FGGKITREPGPLPGISTKITACLDPDGWKSVFVDNIDFLKELE 350

[Close]

BLAST of MC03g0890 vs. ExPASy Swiss-Prot
Match: P46235 (Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) OX=223926 GN=gloA PE=3 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 4.8e-27
Identity = 63/138 (45.65%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 17  NRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPEQSSFVVELIY 76
           N R+LH +  VGDLD++IKFYTE  GM+LLR  +  E +Y+ AF+G+G E    V+EL Y
Sbjct: 3   NGRILHTMLRVGDLDKSIKFYTEVMGMQLLRTNENKEYEYTLAFVGYGDESQGAVIELTY 62

Query: 77  NHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSIIREPFELKGSS 136
           N G T YD+GT FGH AI   D           +Y   + I A +  ++ RE   +KG +
Sbjct: 63  NWGKTEYDLGTAFGHIAIGVDD-----------IYATCDAIKA-AGGNVTREAGPVKGGT 122

Query: 137 FVLAYVKDPDGYIFELIQ 155
             +A+VKDPDGY+ ELIQ
Sbjct: 123 THIAFVKDPDGYMIELIQ 128

[Close]

BLAST of MC03g0890 vs. NCBI nr
Match: XP_022140116.1 (putative lactoylglutathione lyase [Momordica charantia])

HSP 1 Score: 570 bits (1470), Expect = 8.09e-205
Identity = 286/297 (96.30%), Postives = 286/297 (96.30%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF
Sbjct: 8   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 67

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQD           VYKMVENITAK
Sbjct: 68  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQD-----------VYKMVENITAK 127

Query: 121 SADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYE 180
           SADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYE
Sbjct: 128 SADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYE 187

Query: 181 KALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDD 240
           KALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDD
Sbjct: 188 KALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDD 247

Query: 241 VYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           VYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ
Sbjct: 248 VYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 293

[Close]

BLAST of MC03g0890 vs. NCBI nr
Match: XP_038895603.1 (LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Benincasa hispida])

HSP 1 Score: 464 bits (1194), Expect = 9.46e-163
Identity = 235/299 (78.60%), Postives = 255/299 (85.28%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APD   Q+LEWPKKDNRR L  VY VGDLDRTIKFYTECFGM+LLR  +V EEK++ AF
Sbjct: 7   VAPDGHDQMLEWPKKDNRRFLRTVYRVGDLDRTIKFYTECFGMELLRKNEVQEEKHTKAF 66

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MG+GPEQSS V+ELIY HGVTSY+IG GFGHFAIATQD           VYKMVEN+  K
Sbjct: 67  MGYGPEQSSIVLELIYEHGVTSYNIGNGFGHFAIATQD-----------VYKMVENVKDK 126

Query: 121 SADS-IIREPFELKGSS-FVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
             D+ IIREPFE+KGSS FVLAYVKDPDGYIFELIQR PTPQPLCHVMLRV DLQRSI+F
Sbjct: 127 GGDNMIIREPFEVKGSSIFVLAYVKDPDGYIFELIQRDPTPQPLCHVMLRVGDLQRSIDF 186

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           Y+KALGMR LNKV+S+EHKYAIA+MGYADE ET ALELTYNHGVTEHSKG  YSQVAIGT
Sbjct: 187 YKKALGMRVLNKVQSLEHKYAIAMMGYADELETTALELTYNHGVTEHSKGMXYSQVAIGT 246

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           DDVYKSAEVVNLITKELGGKI QQPSL    N+KIT FLDPDGW+IVLVDNEDY+KELQ
Sbjct: 247 DDVYKSAEVVNLITKELGGKINQQPSLASQTNSKITSFLDPDGWQIVLVDNEDYMKELQ 294

[Close]

BLAST of MC03g0890 vs. NCBI nr
Match: XP_022994208.1 (LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Cucurbita maxima])

HSP 1 Score: 450 bits (1158), Expect = 1.53e-156
Identity = 226/298 (75.84%), Postives = 249/298 (83.56%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APDAD QLLEWP KDNR  L AVY V DLDRTI+FYTECFGMKLLRIRDVPE K + AF
Sbjct: 54  VAPDADEQLLEWPMKDNRPFLRAVYRVSDLDRTIEFYTECFGMKLLRIRDVPEAKCTRAF 113

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFG EQSSFV+EL Y++GVTSYDIG GFGHFAIA+QD           VYKMVEN+  K
Sbjct: 114 MGFGSEQSSFVLELNYSYGVTSYDIGAGFGHFAIASQD-----------VYKMVENVRVK 173

Query: 121 SADS--IIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
            A +  ++RE FE+KG S VLA+VKDPDGYIFELIQR PTPQPLC VMLRV DL+RSINF
Sbjct: 174 GAGAGAVVRESFEVKGFSIVLAFVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLERSINF 233

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           YEKALGMR L ++ES+EHKYAIA+MGYADE ET  LELTYNHGVT+HSKGNGYSQVAIGT
Sbjct: 234 YEKALGMRVLTRIESLEHKYAIAMMGYADELETTVLELTYNHGVTQHSKGNGYSQVAIGT 293

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKEL 296
           D V KSAEV+NLI +ELGGKITQQPSL+H   AKIT FLDPDGW+IVLVDNEDYLK L
Sbjct: 294 DKVNKSAEVLNLIIQELGGKITQQPSLVHQFKAKITTFLDPDGWQIVLVDNEDYLKGL 340

[Close]

BLAST of MC03g0890 vs. NCBI nr
Match: XP_004145118.1 (lactoylglutathione lyase GLX1 [Cucumis sativus] >KGN64513.1 hypothetical protein Csa_013535 [Cucumis sativus])

HSP 1 Score: 444 bits (1143), Expect = 5.50e-155
Identity = 226/299 (75.59%), Postives = 252/299 (84.28%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC  MKLLR  +VP EK++ A 
Sbjct: 8   VAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLEMKLLRKYEVPNEKHTKAL 67

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPE+SSF++EL + HGVTSYDIGTGFGHFAIATQD           VYKMVEN+ AK
Sbjct: 68  MGFGPEESSFILELTHEHGVTSYDIGTGFGHFAIATQD-----------VYKMVENVRAK 127

Query: 121 SADS-IIREPFELKGS-SFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
             ++ IIREPFELKGS +F+LAYVKDP+GYIFELIQR  T QPLCH+MLRVADLQRSI+F
Sbjct: 128 GGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRVQT-QPLCHLMLRVADLQRSIDF 187

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           Y+KA+GMR L +VESVE KYAIA+MGYADE ET ALEL YNHGVT+HSKGNGYSQV IGT
Sbjct: 188 YQKAMGMRVLTQVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVVIGT 247

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           DDVYKSAE+VNLITKELGG+ITQ PSL   IN+KIT FLDPDGWKIVLVDNEDYLK +Q
Sbjct: 248 DDVYKSAEIVNLITKELGGEITQPPSLDSQINSKITSFLDPDGWKIVLVDNEDYLKGMQ 294

[Close]

BLAST of MC03g0890 vs. NCBI nr
Match: XP_008440988.1 (PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >ADN34057.1 lactoylglutathione lyase [Cucumis melo subsp. melo] >KAA0056849.1 putative lactoylglutathione lyase [Cucumis melo var. makuwa] >TYJ99352.1 putative lactoylglutathione lyase [Cucumis melo var. makuwa])

HSP 1 Score: 444 bits (1143), Expect = 5.50e-155
Identity = 225/299 (75.25%), Postives = 250/299 (83.61%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC GM+LLR  +V  EK++ A 
Sbjct: 7   VAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNEKHTKAI 66

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPE+SSFV+EL Y  GVTSYDIGTGFGHFAIATQD           VYKMVEN+ AK
Sbjct: 67  MGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQD-----------VYKMVENVRAK 126

Query: 121 SADS-IIREPFELKGSS-FVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
             ++ IIREPFELKGS   +LAYVKDP+GYIFELIQR  TPQPLCH+MLRVADLQRSI+F
Sbjct: 127 GGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDF 186

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           Y KALGMR L KVES+E KYAIA+MGYADE ET A+ELTYNHGVT+HSKGNGYSQVAIGT
Sbjct: 187 YRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGT 246

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           DDVYKSAE+VNLITK+LGGKITQ PSL   +N+KI  FLDPDGW+IVLVDNEDYLK +Q
Sbjct: 247 DDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLKGMQ 294

[Close]

BLAST of MC03g0890 vs. ExPASy TrEMBL
Match: A0A6J1CEU9 (Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111010851 PE=3 SV=1)

HSP 1 Score: 570 bits (1470), Expect = 3.92e-205
Identity = 286/297 (96.30%), Postives = 286/297 (96.30%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF
Sbjct: 8   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 67

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQD           VYKMVENITAK
Sbjct: 68  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQD-----------VYKMVENITAK 127

Query: 121 SADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYE 180
           SADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYE
Sbjct: 128 SADSIIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYE 187

Query: 181 KALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDD 240
           KALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDD
Sbjct: 188 KALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDD 247

Query: 241 VYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           VYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ
Sbjct: 248 VYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 293

[Close]

BLAST of MC03g0890 vs. ExPASy TrEMBL
Match: A0A6J1K4J3 (Glyoxalase I OS=Cucurbita maxima OX=3661 GN=LOC111490014 PE=3 SV=1)

HSP 1 Score: 450 bits (1158), Expect = 7.43e-157
Identity = 226/298 (75.84%), Postives = 249/298 (83.56%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APDAD QLLEWP KDNR  L AVY V DLDRTI+FYTECFGMKLLRIRDVPE K + AF
Sbjct: 54  VAPDADEQLLEWPMKDNRPFLRAVYRVSDLDRTIEFYTECFGMKLLRIRDVPEAKCTRAF 113

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFG EQSSFV+EL Y++GVTSYDIG GFGHFAIA+QD           VYKMVEN+  K
Sbjct: 114 MGFGSEQSSFVLELNYSYGVTSYDIGAGFGHFAIASQD-----------VYKMVENVRVK 173

Query: 121 SADS--IIREPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
            A +  ++RE FE+KG S VLA+VKDPDGYIFELIQR PTPQPLC VMLRV DL+RSINF
Sbjct: 174 GAGAGAVVRESFEVKGFSIVLAFVKDPDGYIFELIQRGPTPQPLCQVMLRVGDLERSINF 233

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           YEKALGMR L ++ES+EHKYAIA+MGYADE ET  LELTYNHGVT+HSKGNGYSQVAIGT
Sbjct: 234 YEKALGMRVLTRIESLEHKYAIAMMGYADELETTVLELTYNHGVTQHSKGNGYSQVAIGT 293

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKEL 296
           D V KSAEV+NLI +ELGGKITQQPSL+H   AKIT FLDPDGW+IVLVDNEDYLK L
Sbjct: 294 DKVNKSAEVLNLIIQELGGKITQQPSLVHQFKAKITTFLDPDGWQIVLVDNEDYLKGL 340

[Close]

BLAST of MC03g0890 vs. ExPASy TrEMBL
Match: A0A5D3BI05 (Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005690 PE=3 SV=1)

HSP 1 Score: 444 bits (1143), Expect = 2.66e-155
Identity = 225/299 (75.25%), Postives = 250/299 (83.61%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC GM+LLR  +V  EK++ A 
Sbjct: 7   VAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNEKHTKAI 66

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPE+SSFV+EL Y  GVTSYDIGTGFGHFAIATQD           VYKMVEN+ AK
Sbjct: 67  MGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQD-----------VYKMVENVRAK 126

Query: 121 SADS-IIREPFELKGSS-FVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
             ++ IIREPFELKGS   +LAYVKDP+GYIFELIQR  TPQPLCH+MLRVADLQRSI+F
Sbjct: 127 GGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDF 186

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           Y KALGMR L KVES+E KYAIA+MGYADE ET A+ELTYNHGVT+HSKGNGYSQVAIGT
Sbjct: 187 YRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGT 246

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           DDVYKSAE+VNLITK+LGGKITQ PSL   +N+KI  FLDPDGW+IVLVDNEDYLK +Q
Sbjct: 247 DDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLKGMQ 294

[Close]

BLAST of MC03g0890 vs. ExPASy TrEMBL
Match: E5GC58 (Glyoxalase I OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 444 bits (1143), Expect = 2.66e-155
Identity = 225/299 (75.25%), Postives = 250/299 (83.61%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC GM+LLR  +V  EK++ A 
Sbjct: 7   VAPDGDEQLLEWPNKDNRRFVRAVYRVGDLDRTIKFYTECLGMELLRKYEVSNEKHTKAI 66

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPE+SSFV+EL Y  GVTSYDIGTGFGHFAIATQD           VYKMVEN+ AK
Sbjct: 67  MGFGPEESSFVLELTYEDGVTSYDIGTGFGHFAIATQD-----------VYKMVENVRAK 126

Query: 121 SADS-IIREPFELKGSS-FVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
             ++ IIREPFELKGS   +LAYVKDP+GYIFELIQR  TPQPLCH+MLRVADLQRSI+F
Sbjct: 127 GGENMIIREPFELKGSPVLLLAYVKDPNGYIFELIQRGQTPQPLCHLMLRVADLQRSIDF 186

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           Y KALGMR L KVES+E KYAIA+MGYADE ET A+ELTYNHGVT+HSKGNGYSQVAIGT
Sbjct: 187 YRKALGMRVLTKVESLEQKYAIALMGYADELETTAVELTYNHGVTQHSKGNGYSQVAIGT 246

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           DDVYKSAE+VNLITK+LGGKITQ PSL   +N+KI  FLDPDGW+IVLVDNEDYLK +Q
Sbjct: 247 DDVYKSAEIVNLITKKLGGKITQPPSLDSQMNSKIISFLDPDGWQIVLVDNEDYLKGMQ 294

[Close]

BLAST of MC03g0890 vs. ExPASy TrEMBL
Match: A0A0A0LS05 (Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_1G062880 PE=3 SV=1)

HSP 1 Score: 444 bits (1143), Expect = 2.66e-155
Identity = 226/299 (75.59%), Postives = 252/299 (84.28%), Query Frame = 0


Query: 1   LAPDADAQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAF 60
           +APD D QLLEWP KDNRR + AVY VGDLDRTIKFYTEC  MKLLR  +VP EK++ A 
Sbjct: 8   VAPDGDEQLLEWPGKDNRRFVRAVYRVGDLDRTIKFYTECLEMKLLRKYEVPNEKHTKAL 67

Query: 61  MGFGPEQSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAK 120
           MGFGPE+SSF++EL + HGVTSYDIGTGFGHFAIATQD           VYKMVEN+ AK
Sbjct: 68  MGFGPEESSFILELTHEHGVTSYDIGTGFGHFAIATQD-----------VYKMVENVRAK 127

Query: 121 SADS-IIREPFELKGS-SFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINF 180
             ++ IIREPFELKGS +F+LAYVKDP+GYIFELIQR  T QPLCH+MLRVADLQRSI+F
Sbjct: 128 GGENMIIREPFELKGSPNFLLAYVKDPNGYIFELIQRVQT-QPLCHLMLRVADLQRSIDF 187

Query: 181 YEKALGMRSLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGT 240
           Y+KA+GMR L +VESVE KYAIA+MGYADE ET ALEL YNHGVT+HSKGNGYSQV IGT
Sbjct: 188 YQKAMGMRVLTQVESVEQKYAIALMGYADELETTALELNYNHGVTQHSKGNGYSQVVIGT 247

Query: 241 DDVYKSAEVVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 297
           DDVYKSAE+VNLITKELGG+ITQ PSL   IN+KIT FLDPDGWKIVLVDNEDYLK +Q
Sbjct: 248 DDVYKSAEIVNLITKELGGEITQPPSLDSQINSKITSFLDPDGWKIVLVDNEDYLKGMQ 294

[Close]

BLAST of MC03g0890 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 402.1 bits (1032), Expect = 3.8e-112
Identity = 192/291 (65.98%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 7   AQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPE 66
           + LLEWPKKDNRR LH VY VGDLDRTI+FYTE FGMKLLR RD+PEEKYSNAF+GFGPE
Sbjct: 5   SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 64

Query: 67  QSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSII 126
            S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V K+VEN+ AK   ++ 
Sbjct: 65  TSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKLVENVRAKGG-NVT 124

Query: 127 REPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMR 186
           REP  +KG   V+A+VKDPDGY FELIQR PTP+P C VMLRV DL R+I FYEKALGMR
Sbjct: 125 REPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMR 184

Query: 187 SLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAE 246
            L K+E  E+KY I +MGYA+E E++ LELTYN+ VTE++KGN Y+Q+AIGTDDVYKS E
Sbjct: 185 LLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 244

Query: 247 VVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           V+ ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDN+D+LKEL+
Sbjct: 245 VIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MC03g0890 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 402.1 bits (1032), Expect = 3.8e-112
Identity = 192/291 (65.98%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 7   AQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPE 66
           + LLEWPKKDNRR LH VY VGDLDRTI+FYTE FGMKLLR RD+PEEKYSNAF+GFGPE
Sbjct: 5   SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 64

Query: 67  QSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSII 126
            S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V K+VEN+ AK   ++ 
Sbjct: 65  TSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKLVENVRAKGG-NVT 124

Query: 127 REPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMR 186
           REP  +KG   V+A+VKDPDGY FELIQR PTP+P C VMLRV DL R+I FYEKALGMR
Sbjct: 125 REPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMR 184

Query: 187 SLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAE 246
            L K+E  E+KY I +MGYA+E E++ LELTYN+ VTE++KGN Y+Q+AIGTDDVYKS E
Sbjct: 185 LLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 244

Query: 247 VVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           V+ ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDN+D+LKEL+
Sbjct: 245 VIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MC03g0890 vs. TAIR 10
Match: AT1G11840.1 (glyoxalase I homolog )

HSP 1 Score: 402.1 bits (1032), Expect = 3.8e-112
Identity = 192/291 (65.98%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 7   AQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPE 66
           + LLEWPKKDNRR LH VY VGDLDRTI+FYTE FGMKLLR RD+PEEKYSNAF+GFGPE
Sbjct: 5   SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 64

Query: 67  QSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSII 126
            S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V K+VEN+ AK   ++ 
Sbjct: 65  TSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKLVENVRAKGG-NVT 124

Query: 127 REPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMR 186
           REP  +KG   V+A+VKDPDGY FELIQR PTP+P C VMLRV DL R+I FYEKALGMR
Sbjct: 125 REPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMR 184

Query: 187 SLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAE 246
            L K+E  E+KY I +MGYA+E E++ LELTYN+ VTE++KGN Y+Q+AIGTDDVYKS E
Sbjct: 185 LLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 244

Query: 247 VVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           V+ ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDN+D+LKEL+
Sbjct: 245 VIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MC03g0890 vs. TAIR 10
Match: AT1G11840.4 (glyoxalase I homolog )

HSP 1 Score: 402.1 bits (1032), Expect = 3.8e-112
Identity = 192/291 (65.98%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 7   AQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPE 66
           + LLEWPKKDNRR LH VY VGDLDRTI+FYTE FGMKLLR RD+PEEKYSNAF+GFGPE
Sbjct: 5   SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 64

Query: 67  QSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSII 126
            S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V K+VEN+ AK   ++ 
Sbjct: 65  TSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKLVENVRAKGG-NVT 124

Query: 127 REPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMR 186
           REP  +KG   V+A+VKDPDGY FELIQR PTP+P C VMLRV DL R+I FYEKALGMR
Sbjct: 125 REPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMR 184

Query: 187 SLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAE 246
            L K+E  E+KY I +MGYA+E E++ LELTYN+ VTE++KGN Y+Q+AIGTDDVYKS E
Sbjct: 185 LLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 244

Query: 247 VVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           V+ ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDN+D+LKEL+
Sbjct: 245 VIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MC03g0890 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 402.1 bits (1032), Expect = 3.8e-112
Identity = 192/291 (65.98%), Postives = 231/291 (79.38%), Query Frame = 0


Query: 7   AQLLEWPKKDNRRLLHAVYCVGDLDRTIKFYTECFGMKLLRIRDVPEEKYSNAFMGFGPE 66
           + LLEWPKKDNRR LH VY VGDLDRTI+FYTE FGMKLLR RD+PEEKYSNAF+GFGPE
Sbjct: 44  SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 103

Query: 67  QSSFVVELIYNHGVTSYDIGTGFGHFAIATQDNINVDLLFISKVYKMVENITAKSADSII 126
            S+FVVEL YN+GV+SYDIGTGFGHFAI+TQD           V K+VEN+ AK   ++ 
Sbjct: 104 TSNFVVELTYNYGVSSYDIGTGFGHFAISTQD-----------VSKLVENVRAKGG-NVT 163

Query: 127 REPFELKGSSFVLAYVKDPDGYIFELIQRAPTPQPLCHVMLRVADLQRSINFYEKALGMR 186
           REP  +KG   V+A+VKDPDGY FELIQR PTP+P C VMLRV DL R+I FYEKALGMR
Sbjct: 164 REPGPVKGGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMR 223

Query: 187 SLNKVESVEHKYAIAIMGYADESETVALELTYNHGVTEHSKGNGYSQVAIGTDDVYKSAE 246
            L K+E  E+KY I +MGYA+E E++ LELTYN+ VTE++KGN Y+Q+AIGTDDVYKS E
Sbjct: 224 LLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGE 283

Query: 247 VVNLITKELGGKITQQPSLIHPINAKITEFLDPDGWKIVLVDNEDYLKELQ 298
           V+ ++ +ELGGKIT++   +  +  KI  FLDPDGWK VLVDN+D+LKEL+
Sbjct: 284 VIKIVNQELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 322

The following BLAST results are available for this feature:

BLAST of MC03g0890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q948T6	2.4e-111	66.44	Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=...	[more]
O65398	5.3e-111	65.98	Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1	[more]
Q39366	8.5e-109	65.75	Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ...	[more]
Q8W593	8.3e-96	59.52	Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
P46235	4.8e-27	45.65	Probable lactoylglutathione lyase OS=Vibrio parahaemolyticus serotype O3:K6 (str...	[more]

BLAST of MC03g0890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140116.1	8.09e-205	96.30	putative lactoylglutathione lyase [Momordica charantia]	[more]
XP_038895603.1	9.46e-163	78.60	LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Benincasa hispida]	[more]
XP_022994208.1	1.53e-156	75.84	LOW QUALITY PROTEIN: lactoylglutathione lyase GLX1 [Cucurbita maxima]	[more]
XP_004145118.1	5.50e-155	75.59	lactoylglutathione lyase GLX1 [Cucumis sativus] >KGN64513.1 hypothetical protein...	[more]
XP_008440988.1	5.50e-155	75.25	PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >ADN34057.1 lactoylg...	[more]

BLAST of MC03g0890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEU9	3.92e-205	96.30	Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111010851 PE=3 SV=...	[more]
A0A6J1K4J3	7.43e-157	75.84	Glyoxalase I OS=Cucurbita maxima OX=3661 GN=LOC111490014 PE=3 SV=1	[more]
A0A5D3BI05	2.66e-155	75.25	Glyoxalase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005690 ...	[more]
E5GC58	2.66e-155	75.25	Glyoxalase I OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A0A0LS05	2.66e-155	75.59	Glyoxalase I OS=Cucumis sativus OX=3659 GN=Csa_1G062880 PE=3 SV=1	[more]

BLAST of MC03g0890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11840.2	3.8e-112	65.98	glyoxalase I homolog	[more]
AT1G11840.3	3.8e-112	65.98	glyoxalase I homolog	[more]
AT1G11840.1	3.8e-112	65.98	glyoxalase I homolog	[more]
AT1G11840.4	3.8e-112	65.98	glyoxalase I homolog	[more]
AT1G11840.6	3.8e-112	65.98	glyoxalase I homolog	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 2..157 e-value: 2.2E-42 score: 146.6
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 158..297 e-value: 3.2E-36 score: 126.6
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 18..157
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 162..289
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 164..284 e-value: 2.8E-10 score: 40.5 coord: 20..152 e-value: 2.0E-12 score: 47.4
IPR004361	Glyoxalase I	TIGRFAM	TIGR00068	TIGR00068	coord: 8..163 e-value: 1.6E-48 score: 161.9
None	No IPR available	PANTHER	PTHR46036	LACTOYLGLUTATHIONE LYASE	coord: 9..297
None	No IPR available	PANTHER	PTHR46036:SF8	LACTOYLGLUTATHIONE LYASE-LIKE PROTEIN	coord: 9..297
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00934	GLYOXALASE_I_1	coord: 164..185
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00935	GLYOXALASE_I_2	coord: 86..98
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 161..289 score: 18.982807
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 19..155 score: 20.710567

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0890.1	MC03g0890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019243	methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004462	lactoylglutathione lyase activity
molecular_function	GO:0046872	metal ion binding