MC03g0867 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0867
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRNA pseudouridine synthase 6, chloroplastic-like
LocationMC03: 15376807 .. 15380670 (-)
RNA-Seq ExpressionMC03g0867
SyntenyMC03g0867
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGGCTTCCATTAATATAGCTCTGGTTGCTGCCATTGGTTGCCGTGGCCTCAGCACATCACAAACCCTGCGACGCACATTCGCAAGAACTCGTGCTAATCTCAGTGCTTTCTGCGACAATTACTTGGCTGTTCCCCGTTCTCCGAAGAGCAGTGTGTTCCGCTGTGAATCCTCAATAGCAGATGTTACTGTTGCACAAACTCCGACTTCTTCTGCAGAAATTGGGTATTGCAATTTTAGACATTTTTAGTTTACTTTGTAGTTTGATGGCTAGTTCTATCTTGTTTCCAGTTTCGAAGTATCGGAAACGGGAGTACTGTTGTTTAGTCGCGCTTTCACTTCGTTTATTCATAGGTAGACCGAAGAAATCATCCAAATTAAATCGTTTCACTGTTAATTTTGTGGTTGTGGGAGATTTCATTGTTAGTCGTCGCAACATTTCTAGTTTTTCTAACACGTTGTCCCATAGAATCTTATTTATCTCTGCAAATTGCTTGTGTTGCGGCAAAAGTGTTGCCGAATGAAAGCAAGTTGGAAGGATTTAAGTTTAAAGAAAGTCCGTGGCTCGAACTATGGTAATTATATATGGACTATGAATAATGAGCATTGAACCCCATATTTACTGGCTAGCACTGAAAATTGTGGTTTTGTTTGGTTCTGTCAGATATGGGAAAAAGTACAATGTTTCTCGACACATTTGCTGATTAGTGATTGCTCCCTTAGCTTGCTTGTTTTATTAATGCACGTGCAGCGATCTATGATTTTTTGTTGGATTCTTTTTCATTTTCCCAACCCCTGTAATTGAATTTCCTTTCTACGATTCCTTGATTCCTTTAGGATTTAGCATGCTCAATCTGAAATTGATTTCAGATTCCCTCAATATCATCGTTTGCTTCCATGTCCCTCACTCAATGGACCACCAAGAGTTGAACATCTGGTCGTTTTGGAAGGGGGGCCTGTCATGGAGTACATCTCTAAATCCCTCGATCTTCCTCCTCTGTACGTTCTATTATCTTTAAGTTGTTCATAATAATTGTTGCTGCAACAATGTTGAAGTCAAATTAAGATGAAGCAATGAATTAAGAGTTTCATTGTTTGAAGGTATGTTGCAGATCTCATTCATTTTGGTGCAGTATATTATGCCCTAGTATGCCCACGGCCCCCGCCAACTGCAACCCCTGAGCAAATTAGACTATTCAAAAAATTTACAGAACCATCATTATTGAGAGGGCGAGCATCTATCAAAGGGAAAACGGTAAGAGAAGCACAGAAGACTTTCCGGATAACTCGTGTTGATGAGTTTATGGAGGTTGGAACGTATTTGCGTGTTCACGTGCATCCTAAACGCTTTCCAAGGTAAAATGAGCCACCTGTTTTTCTTTTCAAAAACGAATATGATGTTCATACTGGGTTGATGCTAGTTGCAATTTACAAGTAATCAGGTGCTATGAAATTGATTGGAAATCAAGAATTATTGCTGTGAGAGAATCCTATGTGGTTTTGGACAAACCTGCTGGTACATCGGTCTGTGGTAACACTTGAACCATTAATCTTTAACTTGTTTTGGTATTGAATTTAAGGAATTAACTTCATTTCCTTATGCATGTGATAGCTGTCGGTTGTTGCATTATTTCTTCTTGTTACATTCTGCAAGATTTGATGAAAACTAGTGCAGGCATGTTGGCGATATGGATAAATTTATTCAATAGGATGGCATAATATATTAACGTCAAAATCATTTAGTTCAGTACTCATTCGTTAGTTATCAAACACATATTTAGCTACTAAACATGACTGGTATTATGTCAGGTTGGAGGCACTACTGACAACATAGAAGAAAGTTGTGCAACTTTTGCAACTCGTGCTTTAGGATTAACATCTCCCTTGTGGACTACCCATCAGATTGACAACTGCACTGAAGGCTGGTAATGTATCTTGTAGGTATTTTTATCTCTACTGCCTTATAATGAATTACTCGTATTAAGTCATTTACACTTTTTTGCAGCATCATTCTGGCAAGGACAAAAGAATATTGCTCTGTCTTTCATCGTAAAATCAGAGTAAGTTTTATTGTATCATTTCTTTCTTCAATTGGGCCATTATAAATTAAGTTACCATACTAACTGGGAAGCCCATTCCATGAAAATGCTGAACTGAAAATGCAGAGTAAGGAAGTTTTAACTTTTAATCCCAGTTCACCCACTCGTTTTTTAGTGCATGCGCATTATTATTTCGATATAGAATAGTTAAATGTTCACACTTAAACCTTCTTTAAGCTCACCATACTGGTAAAAGATAAAAGGGCTCCTACTGTCCTCTCTCTCTAAGTATGAAGTTTATCTATGATAAGAAGGTCCATCAAGGGAACGAGAGTGAAAACTAATCATAAGCTTTGCATTAAGACTGTTATTTTAAACCACTTTGCAAGGAGGTGGTGTAAGATAAAAGGAATAAGGATGGATTTTATTTTGAGATATATTCGTGATAAACAGGGTGCATGAGTTGAGTTATTTTATTGTTGTTGGGAGAATATCTAATATACATATTTGACATTTATTACTTAATTTATTTGTCCCCATCCGTTTCATTTCTTAATTTGATTGGAATGCTTCATATTTTTTTCTTAATAGGAGAAAAAGGTTAAGAAGCTTTATCTTGCTCTTGCTGCTGCTCCTGTGCCAATAGGAATAATAACACACTATATGCGACCAATTAATATGGCTCCAAGACTTGTTTCAGAAGGTAAGTCTCTCAATCAAGCATTGAAATAATAGTTGGATGTTGCTCTGAATTATTTCTTGCAATTTGATATCTTGAGGGGATAGATGTATACCGCTTGATAATTACTTTGCAGCCATTAGCTCTTAGAATATCAGTCTTTAATATTCTGCCAGCAAATTAATGTGCAAATGATTGTCCTTGTGGTATAAATATTTTAACTCCCTCCCAACCCAACACCTACGGTGGTGTCACCCTCAGCCTCCTACCCCAGCCCTAAATGTATCCAGACTAAGATGTGGTTGGCCGTTTAAGTCCTTTTGCTGCTTGCAGATTGTATCGATGGATGGAACTTGTGTCAGCTTGAGGTGTTGGAATGCAGAGAGGTTCCATGGCCGGTTGCTGATATTGAAGCAAAATACTCTATTGAAGAATGTGGGTGGCCATCCAAAAAGAAAGCATATGAATGCAAAATCAACCTCTTGACTGGTCGGACACATCAGGTTCTTAGCGTAGCCATTTTATTTCAGTTGTTACCTCTGTTCTTTGAAAACCATCTTCAGAAATTCTTGTATCCAGACTTTTGACCGTTATTTAAAATTGTTCTTAAAATGCCCCCAAGGGCGGCCCCATGGTTTGAGGTCTCTTAGTCATATTGGTTTAGAGGTTGCAAGTTCGAATTTTTGGGCGAGCTTAATACAAAAAAAACTCTTCATGTCTTCCGGGTCTAAGCTTTGGACGGACATGGATGCCCCTGGGTTTGGGCAACAAAGCTGCCGACTCCGGGAAGAAAGAAAAGAAAAGAAAAGAAAATAAAATTGTTACTAAAATAAATACGTTGTTGTTTCCTGATGATATGCAGATTCGAGCACAATTGGCCGATTGTGGAGCACCATTGGTTGGTGATTCCATGTACATGCCTGCGGCAGTTGCAGAGACGACTAATCCTGGGTTGAATCCATTTGGAAAATGTCAGAAACAGTATACAAGTGAAGAAGATAAAGAAATGGATGTGACAGAGTGGATTGCACGACATGGAAAAGAACCAGCTGTTGCGATTGGACTTCAGGCTTATGAGATTTCTTGGGATGCTGGGGAACACGTTTACAATGCTGGGATCCCTTGGTGGAAGCAAGAACAATTA

mRNA sequence

ATGGTGGCTTCCATTAATATAGCTCTGGTTGCTGCCATTGGTTGCCGTGGCCTCAGCACATCACAAACCCTGCGACGCACATTCGCAAGAACTCGTGCTAATCTCAGTGCTTTCTGCGACAATTACTTGGCTGTTCCCCGTTCTCCGAAGAGCAGTGTGTTCCGCTGTGAATCCTCAATAGCAGATGTTACTGTTGCACAAACTCCGACTTCTTCTGCAGAAATTGGATTCCCTCAATATCATCGTTTGCTTCCATGTCCCTCACTCAATGGACCACCAAGAGTTGAACATCTGGTCGTTTTGGAAGGGGGGCCTGTCATGGAGTACATCTCTAAATCCCTCGATCTTCCTCCTCTGTATGTTGCAGATCTCATTCATTTTGGTGCAGTATATTATGCCCTAGTATGCCCACGGCCCCCGCCAACTGCAACCCCTGAGCAAATTAGACTATTCAAAAAATTTACAGAACCATCATTATTGAGAGGGCGAGCATCTATCAAAGGGAAAACGGTAAGAGAAGCACAGAAGACTTTCCGGATAACTCGTGTTGATGAGTTTATGGAGGTTGGAACGTATTTGCGTGTTCACGTGCATCCTAAACGCTTTCCAAGGTGCTATGAAATTGATTGGAAATCAAGAATTATTGCTGTGAGAGAATCCTATGTGGTTTTGGACAAACCTGCTGGTACATCGGTTGGAGGCACTACTGACAACATAGAAGAAAGTTGTGCAACTTTTGCAACTCGTGCTTTAGGATTAACATCTCCCTTGTGGACTACCCATCAGATTGACAACTGCACTGAAGGCTGCATCATTCTGGCAAGGACAAAAGAATATTGCTCTGTCTTTCATCGTAAAATCAGAGAGAAAAAGGTTAAGAAGCTTTATCTTGCTCTTGCTGCTGCTCCTGTGCCAATAGGAATAATAACACACTATATGCGACCAATTAATATGGCTCCAAGACTTGTTTCAGAAGATTGTATCGATGGATGGAACTTGTGTCAGCTTGAGGTGTTGGAATGCAGAGAGGTTCCATGGCCGGTTGCTGATATTGAAGCAAAATACTCTATTGAAGAATGTGGGTGGCCATCCAAAAAGAAAGCATATGAATGCAAAATCAACCTCTTGACTGGTCGGACACATCAGATTCGAGCACAATTGGCCGATTGTGGAGCACCATTGGTTGGTGATTCCATGTACATGCCTGCGGCAGTTGCAGAGACGACTAATCCTGGGTTGAATCCATTTGGAAAATGTCAGAAACAGTATACAAGTGAAGAAGATAAAGAAATGGATGTGACAGAGTGGATTGCACGACATGGAAAAGAACCAGCTGTTGCGATTGGACTTCAGGCTTATGAGATTTCTTGGGATGCTGGGGAACACGTTTACAATGCTGGGATCCCTTGGTGGAAGCAAGAACAATTA

Coding sequence (CDS)

ATGGTGGCTTCCATTAATATAGCTCTGGTTGCTGCCATTGGTTGCCGTGGCCTCAGCACATCACAAACCCTGCGACGCACATTCGCAAGAACTCGTGCTAATCTCAGTGCTTTCTGCGACAATTACTTGGCTGTTCCCCGTTCTCCGAAGAGCAGTGTGTTCCGCTGTGAATCCTCAATAGCAGATGTTACTGTTGCACAAACTCCGACTTCTTCTGCAGAAATTGGATTCCCTCAATATCATCGTTTGCTTCCATGTCCCTCACTCAATGGACCACCAAGAGTTGAACATCTGGTCGTTTTGGAAGGGGGGCCTGTCATGGAGTACATCTCTAAATCCCTCGATCTTCCTCCTCTGTATGTTGCAGATCTCATTCATTTTGGTGCAGTATATTATGCCCTAGTATGCCCACGGCCCCCGCCAACTGCAACCCCTGAGCAAATTAGACTATTCAAAAAATTTACAGAACCATCATTATTGAGAGGGCGAGCATCTATCAAAGGGAAAACGGTAAGAGAAGCACAGAAGACTTTCCGGATAACTCGTGTTGATGAGTTTATGGAGGTTGGAACGTATTTGCGTGTTCACGTGCATCCTAAACGCTTTCCAAGGTGCTATGAAATTGATTGGAAATCAAGAATTATTGCTGTGAGAGAATCCTATGTGGTTTTGGACAAACCTGCTGGTACATCGGTTGGAGGCACTACTGACAACATAGAAGAAAGTTGTGCAACTTTTGCAACTCGTGCTTTAGGATTAACATCTCCCTTGTGGACTACCCATCAGATTGACAACTGCACTGAAGGCTGCATCATTCTGGCAAGGACAAAAGAATATTGCTCTGTCTTTCATCGTAAAATCAGAGAGAAAAAGGTTAAGAAGCTTTATCTTGCTCTTGCTGCTGCTCCTGTGCCAATAGGAATAATAACACACTATATGCGACCAATTAATATGGCTCCAAGACTTGTTTCAGAAGATTGTATCGATGGATGGAACTTGTGTCAGCTTGAGGTGTTGGAATGCAGAGAGGTTCCATGGCCGGTTGCTGATATTGAAGCAAAATACTCTATTGAAGAATGTGGGTGGCCATCCAAAAAGAAAGCATATGAATGCAAAATCAACCTCTTGACTGGTCGGACACATCAGATTCGAGCACAATTGGCCGATTGTGGAGCACCATTGGTTGGTGATTCCATGTACATGCCTGCGGCAGTTGCAGAGACGACTAATCCTGGGTTGAATCCATTTGGAAAATGTCAGAAACAGTATACAAGTGAAGAAGATAAAGAAATGGATGTGACAGAGTGGATTGCACGACATGGAAAAGAACCAGCTGTTGCGATTGGACTTCAGGCTTATGAGATTTCTTGGGATGCTGGGGAACACGTTTACAATGCTGGGATCCCTTGGTGGAAGCAAGAACAATTA

Protein sequence

MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL
Homology
[Close]
BLAST of MC03g0867 vs. ExPASy Swiss-Prot
Match: Q9SVS0 (RNA pseudouridine synthase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g21770 PE=2 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 1.1e-182
Identity = 318/467 (68.09%), Postives = 370/467 (79.23%), Query Frame = 0 

Query: 14  GCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPR---SPKSSVFRCESSIADVTVAQTP- 73
           G R L+   +L RT A TR     F  N  + PR   SPK   F C S +   +  QT  
Sbjct: 9   GYRNLTAPVSLLRTLASTRVTTPLFRSNKHS-PRFISSPKR--FTCLSLLKTDSQNQTTL 68

Query: 74  TSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGP-VMEYISKSLDLPPLYVADLIHFG 133
           +SS+  G+ +Y+RL+PCP+ N PPR+EH+VVLE    V E+ISK LDLPPLYVADLI FG
Sbjct: 69  SSSSNSGYHEYNRLMPCPAYNLPPRIEHMVVLEDDVLVSEFISKQLDLPPLYVADLIRFG 128

Query: 134 AVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRITRVDEFME 193
           AV+YALVCP+PPPTATPE+I LF++ T PS+L+ R+SIKGKTVREAQKTFR+T  +++ E
Sbjct: 129 AVHYALVCPKPPPTATPEEIILFEEVTSPSVLKKRSSIKGKTVREAQKTFRVTHTNQYAE 188

Query: 194 VGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIEESCATFAT 253
            GTYLRVHVHPKR PRCYEIDWKSRI+AV +SYV+LDKPAGT+VGGTTDNIEESCATFA+
Sbjct: 189 AGTYLRVHVHPKRSPRCYEIDWKSRIVAVTDSYVILDKPAGTTVGGTTDNIEESCATFAS 248

Query: 254 RALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALAAAPVPIGI 313
           RAL L  PL TTHQIDNCTEGC++ ARTKEYCSVFH KIR K+VKKLY ALAAAP+PIGI
Sbjct: 249 RALDLPEPLKTTHQIDNCTEGCVVFARTKEYCSVFHTKIRNKEVKKLYRALAAAPLPIGI 308

Query: 314 ITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEECGWPSKKKA 373
           I+HYMRP NMAPRLV+ED I GW+LCQLEVLEC+++PWP A  E K+ IE+CGW SK+ A
Sbjct: 309 ISHYMRPKNMAPRLVAEDSIQGWHLCQLEVLECKKIPWPDAATEKKHDIEDCGWTSKEFA 368

Query: 374 YECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQKQYTSEED 433
           YEC INLLTG+THQIRAQLA CGAPLVGDSMYMPAA+AE  NP  NP+GK +K YT EE+
Sbjct: 369 YECTINLLTGKTHQIRAQLAACGAPLVGDSMYMPAAIAEMGNPDTNPYGKAKKHYTMEEN 428

Query: 434 -KEMDVTEWIARHGKEPAVAIGLQAYEISW--DAGEHVYNAGIPWWK 473
            KE  V EWI RHGKEP V IGLQA +ISW  D GEH Y AG PWW+
Sbjct: 429 QKETAVAEWIDRHGKEPRVGIGLQACQISWDDDDGEHFYEAGTPWWR 472

[Close]
BLAST of MC03g0867 vs. ExPASy Swiss-Prot
Match: A3BN26 (RNA pseudouridine synthase 6, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0660400 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.5e-163
Identity = 271/419 (64.68%), Postives = 327/419 (78.04%), Query Frame = 0 

Query: 58  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 117
           S+ A  T A T  + A   +P Y RLLPCP  + PPR+EHLV  E    +++IS+SL LP
Sbjct: 52  SAAAVSTSAATVEAPATAAYPVYGRLLPCPLQDDPPRIEHLVAREDEVAVDFISRSLTLP 111

Query: 118 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 177
           PLYVADLI FGAVYYALV P+PPP A PE +R+F++ TEPS+L  R SIKGKTVREAQKT
Sbjct: 112 PLYVADLIKFGAVYYALVAPQPPPHAAPEHVRIFREVTEPSVLCRRKSIKGKTVREAQKT 171

Query: 178 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 237
           FR+T  ++ +E GTYLRVHVHPKRFPRCYEIDWKSR+IAV ++YVVLDKPA TSVGG TD
Sbjct: 172 FRVTDPNQRLEAGTYLRVHVHPKRFPRCYEIDWKSRVIAVTDNYVVLDKPAATSVGGATD 231

Query: 238 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 297
           NIEESC  F +RALGL +PL TTHQIDNC+EGC++L++TKE+CSVFH  IREK+V K YL
Sbjct: 232 NIEESCVVFTSRALGLETPLMTTHQIDNCSEGCVVLSKTKEFCSVFHGMIREKQVNKRYL 291

Query: 298 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 357
           AL  APV  GIITHYMRPIN APRLVSED I GW++CQ+E+L+C++VPWP + I   + +
Sbjct: 292 ALTTAPVSTGIITHYMRPINRAPRLVSEDHIKGWHVCQMEILDCKKVPWPSSLIRKVHKV 351

Query: 358 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 417
           + CGWP ++ AYECKINLLTG+THQIRAQLA  G P+VGDS YM AA+A   NP +NPFG
Sbjct: 352 DNCGWPQQEAAYECKINLLTGKTHQIRAQLAAIGTPIVGDSAYMTAAMAAIVNPSINPFG 411

Query: 418 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWD-AGE-HVYNAGIPWWKQE 475
           +  + Y SE++K   V  WI+ HGKEP   IGLQA EISWD  GE H Y AG+PWW+Q+
Sbjct: 412 RWGQNYDSEDEKAAAVEAWISCHGKEPKSVIGLQASEISWDYEGEHHSYKAGVPWWRQD 470

[Close]
BLAST of MC03g0867 vs. ExPASy Swiss-Prot
Match: P43930 (Uncharacterized RNA pseudouridine synthase HI_0042 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=HI_0042 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 7.2e-09
Identity = 47/194 (24.23%), Postives = 84/194 (43.30%), Query Frame = 0 

Query: 214 IIAVRESYVVLDKPAGTSVGGTTDNIEESCATFATRALGLTSPLWTTHQIDNCTEGCIIL 273
           ++     +++++K  G SV    D  E+       + L +   +W  H++D  T G +IL
Sbjct: 5   VVYQHSDFIIINKSEGISV--HKDQEEQGLTELVAKQLNVPK-VWLVHRLDKVTSGLLIL 64

Query: 274 ARTKEYCSVFHRKIREKKVKKLYLALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNL 333
           A   E  + F R   E K+ K YLAL+                N  P+   +  I G   
Sbjct: 65  ALNAESAAEFSRLFSEHKIHKTYLALS----------------NQKPK-KKQGLIIG--- 124

Query: 334 CQLEVLECREVPWPVADIEAKYSIEECGWPSKKKAYECKINL-------LTGRTHQIRAQ 393
              ++ + RE  W +   +   +I      ++ ++  C+ NL        TG+THQ+R  
Sbjct: 125 ---DMKKAREGAWKLCQTKDNPAI------TRFESVSCEPNLRLFILKPQTGKTHQLRVA 166

Query: 394 LADCGAPLVGDSMY 401
           +   G+P++GD +Y
Sbjct: 185 MKSLGSPILGDGLY 166

[Close]
BLAST of MC03g0867 vs. ExPASy Swiss-Prot
Match: P75485 (Uncharacterized RNA pseudouridine synthase MG209 homolog OS=Mycoplasma pneumoniae (strain ATCC 29342 / M129) OX=272634 GN=MPN_292 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-07
Identity = 64/249 (25.70%), Postives = 103/249 (41.37%), Query Frame = 0 

Query: 159 LLRGRASIKGKTVRE----AQKTFRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRI 218
           ++ G  S+ GK + +     Q   R+  V+   E+   + V V P  FP    +D    I
Sbjct: 32  IVDGLVSVNGKKITKNGWLVQPEDRV-HVNWSEELFEKVPVEVQPYDFP----LD----I 91

Query: 219 IAVRESYVVLDKPAGTSVGGTTDNIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILA 278
           +   E  +V++KP G     T+ N  ES    A        P++  H++D  T G I+LA
Sbjct: 92  LYEDEQIMVVNKPNGLISHPTSFNESESLLGAALFHCN-HQPVFLVHRLDRDTSGVIMLA 151

Query: 279 RTKEYCSVFHRKIREKKVKKLYLALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLC 338
           + +       ++++++ +K+ YLAL   P+                     D + G    
Sbjct: 152 KNQSSLLHLQKQLQQRVMKRYYLALVHFPL---------------------DSLSGTISA 211

Query: 339 QLEVLECREVPWPVADIEAKYSIEECGWP---SKKKAYECKINLLTGRTHQIRAQLADCG 398
            LE +   +V W V +   K       +      +KA   K  LLTGRTHQIR  L    
Sbjct: 212 PLERVGNNKVMWKVGNSSNKAKNAFTKFTVLNQNEKAALIKCELLTGRTHQIRVHLQFIK 249

Query: 399 APLVGDSMY 401
            P+  D +Y
Sbjct: 272 HPVYNDPLY 249

[Close]
BLAST of MC03g0867 vs. ExPASy Swiss-Prot
Match: Q8PHN2 (Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. citri (strain 306) OX=190486 GN=rluD PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.4e-07
Identity = 51/195 (26.15%), Postives = 76/195 (38.97%), Query Frame = 0 

Query: 219 ESYVVLDKPAGTSV---GGTTDNIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILAR 278
           E  +V+DKPAG  V    G  D    +   F   AL         H++D  T G +++AR
Sbjct: 97  EQVLVIDKPAGLVVHPGAGNPDRTLVNALLFRDPALASVPRAGVVHRLDKDTSGVMVVAR 156

Query: 279 TKEYCSVFHRKIREKKVKKLYLALAAAPVPIG------IITHYMRPINMAPRLVSEDCID 338
           T +  +    ++  ++V + YLA+    +  G      I  H    + MA R    D + 
Sbjct: 157 TVQAQTALVEQLSAREVHRQYLAVVVGALVSGGTANAPIDRHPRDRLKMAVRDDGRDAVT 216

Query: 339 GWNLCQL----EVLECREVPWPVADIEAKYSIEECGWPSKKKAYECKINLLTGRTHQIRA 398
            + L +       LECR                                L TGRTHQIR 
Sbjct: 217 HYRLRERFRAHTALECR--------------------------------LETGRTHQIRV 259

Query: 399 QLADCGAPLVGDSMY 401
            +A   +P+VGD +Y
Sbjct: 277 HMAHLKSPIVGDPLY 259

[Close]
BLAST of MC03g0867 vs. NCBI nr
Match: XP_022139955.1 (RNA pseudouridine synthase 6, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 984 bits (2545), Expect = 0.0
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSI 60
           MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSI
Sbjct: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSI 60

Query: 61  ADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLY 120
           ADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLY
Sbjct: 61  ADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLY 120

Query: 121 VADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRI 180
           VADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRI
Sbjct: 121 VADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRI 180

Query: 181 TRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIE 240
           TRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIE
Sbjct: 181 TRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIE 240

Query: 241 ESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALA 300
           ESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALA
Sbjct: 241 ESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALA 300

Query: 301 AAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEEC 360
           AAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEEC
Sbjct: 301 AAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEEC 360

Query: 361 GWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQ 420
           GWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQ
Sbjct: 361 GWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQ 420

Query: 421 KQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL
Sbjct: 421 KQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476

[Close]
BLAST of MC03g0867 vs. NCBI nr
Match: XP_022994289.1 (RNA pseudouridine synthase 6, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022994290.1 RNA pseudouridine synthase 6, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022994291.1 RNA pseudouridine synthase 6, chloroplastic-like isoform X1 [Cucurbita maxima] >XP_022994292.1 RNA pseudouridine synthase 6, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 418/479 (87.27%), Postives = 443/479 (92.48%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSA-FCDNYLAVPRS--PKSSVFRCE 60
           M+AS+NIALVAA GCRGLSTS  L+RT ARTRAN SA  C NY A  RS   KSS F CE
Sbjct: 1   MLASMNIALVAANGCRGLSTSLALQRTLARTRANFSATVCGNYFAARRSLSSKSSAFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
           SS+ DV V QT T+ AE G+PQYHRLLPCPS +GPPRVEHLVVLEGGPVMEYI+KSLDLP
Sbjct: 61  SSVTDVAVTQTATTFAESGYPQYHRLLPCPSFSGPPRVEHLVVLEGGPVMEYIAKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAVYYALVCP+PPPTATPEQIRLFKKFTEPS L+GR SIKGKTVREAQKT
Sbjct: 121 PLYVADLIHFGAVYYALVCPQPPPTATPEQIRLFKKFTEPSFLKGRKSIKGKTVREAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+DEF+EVGTYLR+HVHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTTD
Sbjct: 181 FRITRIDEFVEVGTYLRIHVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTD 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEESCATFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCSVFHRKIREKKVKKLYL
Sbjct: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSVFHRKIREKKVKKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVPIGIITHYMRPIN+APRLVSEDCIDG+NLCQLEVLECREV WP ADIEAKY I
Sbjct: 301 ALAAAPVPIGIITHYMRPINIAPRLVSEDCIDGYNLCQLEVLECREVSWPGADIEAKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           EECGWPSK KAYECKINLLTGRTHQIRAQLA+CGAPLVGDSMYMPAAVAE  NPGL+PFG
Sbjct: 361 EECGWPSKTKAYECKINLLTGRTHQIRAQLANCGAPLVGDSMYMPAAVAEMANPGLSPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KC+K+Y SEEDKE+ VTEWI+RHGKEPA+AIGLQAYEISWD+ EH+YNAG PWWKQEQL
Sbjct: 421 KCKKEYASEEDKEIAVTEWISRHGKEPAMAIGLQAYEISWDSMEHIYNAGTPWWKQEQL 479

[Close]
BLAST of MC03g0867 vs. NCBI nr
Match: XP_022954964.1 (RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita moschata] >XP_022954965.1 RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita moschata] >XP_022954966.1 RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita moschata] >XP_022954967.1 RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 865 bits (2235), Expect = 3.21e-315
Identity = 417/479 (87.06%), Postives = 441/479 (92.07%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAF-CDNYLAVPRS--PKSSVFRCE 60
           M+AS+NIALVAA GCRGL TS  L+RT ARTRAN SA  C NY A  RS   KSS F CE
Sbjct: 1   MLASMNIALVAANGCRGLGTSLALQRTLARTRANFSATACGNYFAARRSLSSKSSAFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
           SS+ DV V Q+ T+ AE G+PQYHRLLPCPS +GPPRVEHLVVLEGGPVMEYISKSLDLP
Sbjct: 61  SSVTDVAVTQSTTTFAENGYPQYHRLLPCPSFSGPPRVEHLVVLEGGPVMEYISKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAVYYALVCP+PPPTATPEQIRLFKKFT+PS L+GR SIKGKTVREAQKT
Sbjct: 121 PLYVADLIHFGAVYYALVCPQPPPTATPEQIRLFKKFTQPSFLKGRKSIKGKTVREAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+DEF+EVGTYLR+HVHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTTD
Sbjct: 181 FRITRIDEFVEVGTYLRIHVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTD 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEESCATFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCSVFHRKIREKKVKKLYL
Sbjct: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSVFHRKIREKKVKKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVPIGIITHYMRPIN+APRLVSEDCIDG+NLCQLEVLECREV WP ADIEAKY I
Sbjct: 301 ALAAAPVPIGIITHYMRPINIAPRLVSEDCIDGYNLCQLEVLECREVAWPDADIEAKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           EECGWPSK KAYECKINLLTGRTHQIRAQLA+CGAPLVGDSMYMPAAVAE  NPGLNPFG
Sbjct: 361 EECGWPSKTKAYECKINLLTGRTHQIRAQLANCGAPLVGDSMYMPAAVAEMANPGLNPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KC+K+Y SEEDKE  VTEWI+RHGKEPA+AIGLQAYEISWD+ EH+YNAG PWWKQEQL
Sbjct: 421 KCKKEYASEEDKESAVTEWISRHGKEPAMAIGLQAYEISWDSMEHIYNAGTPWWKQEQL 479

[Close]
BLAST of MC03g0867 vs. NCBI nr
Match: KAG6573200.1 (RNA pseudouridine synthase 6, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 862 bits (2228), Expect = 3.68e-314
Identity = 414/479 (86.43%), Postives = 442/479 (92.28%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSA-FCDNYLAVPRS--PKSSVFRCE 60
           M+AS+NIALVAA GCRGL TS  L+RT ARTRAN SA  C NY A  RS   KSS F CE
Sbjct: 2   MLASMNIALVAANGCRGLGTSLALQRTLARTRANFSASVCGNYFAARRSLSSKSSAFPCE 61

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
           SS+ DV V ++ T+ AE G+PQYHRLLPCPS +GPPRVEHLVVLEGGPVMEYISKSLDLP
Sbjct: 62  SSVTDVAVTKSATTFAENGYPQYHRLLPCPSFSGPPRVEHLVVLEGGPVMEYISKSLDLP 121

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAVYYALVCP+PPPTATPEQIRLFKKFT+PS L+GR SIKGKTVREAQKT
Sbjct: 122 PLYVADLIHFGAVYYALVCPQPPPTATPEQIRLFKKFTQPSFLKGRKSIKGKTVREAQKT 181

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+DEF+EVGTYLR+HVHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTTD
Sbjct: 182 FRITRIDEFVEVGTYLRIHVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTD 241

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEESCATFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCSVFHRKIREKKVKKLYL
Sbjct: 242 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSVFHRKIREKKVKKLYL 301

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVPIGIITHYMRPIN+APRLVSEDCIDG+NLCQLEVLECREV WP ADIEAKY I
Sbjct: 302 ALAAAPVPIGIITHYMRPINIAPRLVSEDCIDGYNLCQLEVLECREVAWPDADIEAKYCI 361

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           EECGWPSK KAYECKINLLTGRTHQIRAQLA+CGAPLVGDSMYMPAAVAE  NPGLNPFG
Sbjct: 362 EECGWPSKTKAYECKINLLTGRTHQIRAQLANCGAPLVGDSMYMPAAVAEMANPGLNPFG 421

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KC+K+Y +EEDKE+ VT+WI+RHGKEPA+AIGLQAYEISWD+ EH+YNAG PWWKQEQL
Sbjct: 422 KCKKEYANEEDKEIAVTDWISRHGKEPAMAIGLQAYEISWDSMEHIYNAGTPWWKQEQL 480

[Close]
BLAST of MC03g0867 vs. NCBI nr
Match: XP_023542657.1 (RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023542658.1 RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023542659.1 RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023542660.1 RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 861 bits (2225), Expect = 1.01e-313
Identity = 416/479 (86.85%), Postives = 439/479 (91.65%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSA-FCDNYLAVPRS--PKSSVFRCE 60
           M+AS+NIALVAA GCRGL TS  L+RT ARTRAN SA  C NY A  RS   KSS F CE
Sbjct: 1   MLASMNIALVAANGCRGLGTSLALQRTLARTRANFSATVCGNYFAARRSLSSKSSAFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
           SS+ DV V QT T+ AE G+ QYHRLLPCPS +GPPRVEHLVVLEGGPVMEYISKSLDLP
Sbjct: 61  SSVTDVAVTQTATTFAENGYSQYHRLLPCPSFSGPPRVEHLVVLEGGPVMEYISKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAVYYALVCP+PPPTATPEQIRLFKKFTEPS L+GR SIKGKTVREAQKT
Sbjct: 121 PLYVADLIHFGAVYYALVCPQPPPTATPEQIRLFKKFTEPSFLKGRKSIKGKTVREAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+DEF+EVGTYLR+HVHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTTD
Sbjct: 181 FRITRIDEFVEVGTYLRIHVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTD 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEESCATFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCSVFHRKIREKKVKKLYL
Sbjct: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSVFHRKIREKKVKKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVPIGIITHYMRPIN+APRLVSEDCIDG+NLCQLE LECREV WP ADIE KY I
Sbjct: 301 ALAAAPVPIGIITHYMRPINIAPRLVSEDCIDGYNLCQLEGLECREVAWPDADIEEKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           EECGWPSK KAYECKINLLTGRTHQIRAQLA+CGAPLVGDSMYMPAAVAE  NPGLNPFG
Sbjct: 361 EECGWPSKTKAYECKINLLTGRTHQIRAQLANCGAPLVGDSMYMPAAVAEMANPGLNPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KC+K+Y SEEDKE+ VTEWI+RHGKEPA+AIGLQAYEISWD+ EH+YNAG PWWKQEQL
Sbjct: 421 KCKKEYASEEDKEIAVTEWISRHGKEPAMAIGLQAYEISWDSMEHIYNAGTPWWKQEQL 479

[Close]
BLAST of MC03g0867 vs. ExPASy TrEMBL
Match: A0A6J1CDQ3 (RNA pseudouridine synthase 6, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010740 PE=4 SV=1)

HSP 1 Score: 984 bits (2545), Expect = 0.0
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSI 60
           MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSI
Sbjct: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPRSPKSSVFRCESSI 60

Query: 61  ADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLY 120
           ADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLY
Sbjct: 61  ADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLPPLY 120

Query: 121 VADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRI 180
           VADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRI
Sbjct: 121 VADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRI 180

Query: 181 TRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIE 240
           TRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIE
Sbjct: 181 TRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIE 240

Query: 241 ESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALA 300
           ESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALA
Sbjct: 241 ESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALA 300

Query: 301 AAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEEC 360
           AAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEEC
Sbjct: 301 AAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEEC 360

Query: 361 GWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQ 420
           GWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQ
Sbjct: 361 GWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQ 420

Query: 421 KQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL
Sbjct: 421 KQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476

[Close]
BLAST of MC03g0867 vs. ExPASy TrEMBL
Match: A0A6J1JVD9 (RNA pseudouridine synthase 6, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490064 PE=4 SV=1)

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 418/479 (87.27%), Postives = 443/479 (92.48%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSA-FCDNYLAVPRS--PKSSVFRCE 60
           M+AS+NIALVAA GCRGLSTS  L+RT ARTRAN SA  C NY A  RS   KSS F CE
Sbjct: 1   MLASMNIALVAANGCRGLSTSLALQRTLARTRANFSATVCGNYFAARRSLSSKSSAFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
           SS+ DV V QT T+ AE G+PQYHRLLPCPS +GPPRVEHLVVLEGGPVMEYI+KSLDLP
Sbjct: 61  SSVTDVAVTQTATTFAESGYPQYHRLLPCPSFSGPPRVEHLVVLEGGPVMEYIAKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAVYYALVCP+PPPTATPEQIRLFKKFTEPS L+GR SIKGKTVREAQKT
Sbjct: 121 PLYVADLIHFGAVYYALVCPQPPPTATPEQIRLFKKFTEPSFLKGRKSIKGKTVREAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+DEF+EVGTYLR+HVHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTTD
Sbjct: 181 FRITRIDEFVEVGTYLRIHVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTD 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEESCATFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCSVFHRKIREKKVKKLYL
Sbjct: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSVFHRKIREKKVKKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVPIGIITHYMRPIN+APRLVSEDCIDG+NLCQLEVLECREV WP ADIEAKY I
Sbjct: 301 ALAAAPVPIGIITHYMRPINIAPRLVSEDCIDGYNLCQLEVLECREVSWPGADIEAKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           EECGWPSK KAYECKINLLTGRTHQIRAQLA+CGAPLVGDSMYMPAAVAE  NPGL+PFG
Sbjct: 361 EECGWPSKTKAYECKINLLTGRTHQIRAQLANCGAPLVGDSMYMPAAVAEMANPGLSPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KC+K+Y SEEDKE+ VTEWI+RHGKEPA+AIGLQAYEISWD+ EH+YNAG PWWKQEQL
Sbjct: 421 KCKKEYASEEDKEIAVTEWISRHGKEPAMAIGLQAYEISWDSMEHIYNAGTPWWKQEQL 479

[Close]
BLAST of MC03g0867 vs. ExPASy TrEMBL
Match: A0A6J1GUJ2 (RNA pseudouridine synthase 6, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457066 PE=4 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 1.55e-315
Identity = 417/479 (87.06%), Postives = 441/479 (92.07%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLSAF-CDNYLAVPRS--PKSSVFRCE 60
           M+AS+NIALVAA GCRGL TS  L+RT ARTRAN SA  C NY A  RS   KSS F CE
Sbjct: 1   MLASMNIALVAANGCRGLGTSLALQRTLARTRANFSATACGNYFAARRSLSSKSSAFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
           SS+ DV V Q+ T+ AE G+PQYHRLLPCPS +GPPRVEHLVVLEGGPVMEYISKSLDLP
Sbjct: 61  SSVTDVAVTQSTTTFAENGYPQYHRLLPCPSFSGPPRVEHLVVLEGGPVMEYISKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAVYYALVCP+PPPTATPEQIRLFKKFT+PS L+GR SIKGKTVREAQKT
Sbjct: 121 PLYVADLIHFGAVYYALVCPQPPPTATPEQIRLFKKFTQPSFLKGRKSIKGKTVREAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+DEF+EVGTYLR+HVHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTTD
Sbjct: 181 FRITRIDEFVEVGTYLRIHVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTD 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEESCATFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCSVFHRKIREKKVKKLYL
Sbjct: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSVFHRKIREKKVKKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVPIGIITHYMRPIN+APRLVSEDCIDG+NLCQLEVLECREV WP ADIEAKY I
Sbjct: 301 ALAAAPVPIGIITHYMRPINIAPRLVSEDCIDGYNLCQLEVLECREVAWPDADIEAKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           EECGWPSK KAYECKINLLTGRTHQIRAQLA+CGAPLVGDSMYMPAAVAE  NPGLNPFG
Sbjct: 361 EECGWPSKTKAYECKINLLTGRTHQIRAQLANCGAPLVGDSMYMPAAVAEMANPGLNPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           KC+K+Y SEEDKE  VTEWI+RHGKEPA+AIGLQAYEISWD+ EH+YNAG PWWKQEQL
Sbjct: 421 KCKKEYASEEDKESAVTEWISRHGKEPAMAIGLQAYEISWDSMEHIYNAGTPWWKQEQL 479

[Close]
BLAST of MC03g0867 vs. ExPASy TrEMBL
Match: A0A6J1F482 (RNA pseudouridine synthase 6, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111440038 PE=4 SV=1)

HSP 1 Score: 835 bits (2158), Expect = 7.87e-304
Identity = 403/479 (84.13%), Postives = 431/479 (89.98%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLS-AFCDNYLAVPRSP--KSSVFRCE 60
           M+AS+NIALVAA GC GL TS+TL RT ARTRANL  A C N  A  RS   KSSVF CE
Sbjct: 1   MLASMNIALVAAHGCHGLITSRTLLRTLARTRANLGVAICGNCFAARRSSSSKSSVFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
            S  D+TV QT T SAE G+PQYHRLLPCPS   PPRVEHLVVLE GPVMEYISKSLDLP
Sbjct: 61  CSTTDITVTQTATDSAENGYPQYHRLLPCPSFKEPPRVEHLVVLEEGPVMEYISKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAV+YALVCPRPPPTATPEQIRLFKKFTEP+ L+GR SIKGKT+ EAQKT
Sbjct: 121 PLYVADLIHFGAVHYALVCPRPPPTATPEQIRLFKKFTEPTFLKGRKSIKGKTMGEAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRITR+D+F+EVG YLRV+VHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTT+
Sbjct: 181 FRITRIDDFVEVGMYLRVYVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTN 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           NIEE+C TFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCS+FHRKIREKKV KLYL
Sbjct: 241 NIEETCVTFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSIFHRKIREKKVMKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVP+GIITHYMRPIN+APRLVS+DCIDGWNLCQLEVLECREV WPVADIEAKY I
Sbjct: 301 ALAAAPVPMGIITHYMRPINLAPRLVSKDCIDGWNLCQLEVLECREVSWPVADIEAKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           E+CGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDS+YMPAAVAE  NPG NPFG
Sbjct: 361 EDCGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSIYMPAAVAEMANPGSNPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           + +K+YTSEEDKE+ V EWIA HGKEPA+AIGLQAYEISWD+GEH YNAG PWW Q QL
Sbjct: 421 RSKKEYTSEEDKEIAVAEWIALHGKEPALAIGLQAYEISWDSGEHSYNAGTPWWNQGQL 479

[Close]
BLAST of MC03g0867 vs. ExPASy TrEMBL
Match: A0A6J1KKP4 (RNA pseudouridine synthase 6, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495443 PE=4 SV=1)

HSP 1 Score: 823 bits (2126), Expect = 5.89e-299
Identity = 396/479 (82.67%), Postives = 427/479 (89.14%), Query Frame = 0 

Query: 1   MVASINIALVAAIGCRGLSTSQTLRRTFARTRANLS-AFCDNYLAVPRSP--KSSVFRCE 60
           M+AS+NIALVAA GC  L TS+TL  T ARTR N   A C N  A  RS   KSSVF CE
Sbjct: 1   MLASMNIALVAAHGCHDLITSRTLSCTLARTRTNPGVAICGNCFAARRSSSSKSSVFPCE 60

Query: 61  SSIADVTVAQTPTSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGPVMEYISKSLDLP 120
            S  D+TV QT T+SAE G+PQYHRLLPCPS   PPRVEHLVVLE GPVMEYISKSLDLP
Sbjct: 61  CSTTDITVTQTATNSAENGYPQYHRLLPCPSFKEPPRVEHLVVLEEGPVMEYISKSLDLP 120

Query: 121 PLYVADLIHFGAVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKT 180
           PLYVADLIHFGAV+YALVCPRPPPTATPEQIRLFKKFTEP+ L+GR SIKGKT+ EAQKT
Sbjct: 121 PLYVADLIHFGAVHYALVCPRPPPTATPEQIRLFKKFTEPTFLKGRKSIKGKTMGEAQKT 180

Query: 181 FRITRVDEFMEVGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTD 240
           FRIT +D+F+EVG YLRV+VHPKRFPRCYEIDWKSRIIAV ESYVVLDKPAGTSVGGTT+
Sbjct: 181 FRITHIDDFVEVGMYLRVYVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTN 240

Query: 241 NIEESCATFATRALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYL 300
           N+EE+C TFATRALGLTSPLWTTHQIDNCTEGC+ILARTKEYCS+FHRKIREKKV KLYL
Sbjct: 241 NVEETCVTFATRALGLTSPLWTTHQIDNCTEGCVILARTKEYCSIFHRKIREKKVMKLYL 300

Query: 301 ALAAAPVPIGIITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSI 360
           ALAAAPVP+GIITHYMRPIN+APR+VS+DCIDGWNLCQLEVLECREV WPVADIEAKY I
Sbjct: 301 ALAAAPVPMGIITHYMRPINLAPRIVSKDCIDGWNLCQLEVLECREVSWPVADIEAKYCI 360

Query: 361 EECGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFG 420
           E+CGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDS+YMPAAVAE  NPG NPFG
Sbjct: 361 EDCGWPSKKKAYECKINLLTGRTHQIRAQLADCGAPLVGDSIYMPAAVAEMANPGSNPFG 420

Query: 421 KCQKQYTSEEDKEMDVTEWIARHGKEPAVAIGLQAYEISWDAGEHVYNAGIPWWKQEQL 476
           + +K+YTSEEDKE+ V EWIA HGKEPA+AIGLQAYEISWD+GEH YNAG PWW Q QL
Sbjct: 421 RSKKEYTSEEDKEIAVAEWIALHGKEPALAIGLQAYEISWDSGEHSYNAGTPWWNQGQL 479

[Close]
BLAST of MC03g0867 vs. TAIR 10
Match: AT4G21770.1 (Pseudouridine synthase family protein )

HSP 1 Score: 641.0 bits (1652), Expect = 7.8e-184
Identity = 318/467 (68.09%), Postives = 370/467 (79.23%), Query Frame = 0 

Query: 14  GCRGLSTSQTLRRTFARTRANLSAFCDNYLAVPR---SPKSSVFRCESSIADVTVAQTP- 73
           G R L+   +L RT A TR     F  N  + PR   SPK   F C S +   +  QT  
Sbjct: 9   GYRNLTAPVSLLRTLASTRVTTPLFRSNKHS-PRFISSPKR--FTCLSLLKTDSQNQTTL 68

Query: 74  TSSAEIGFPQYHRLLPCPSLNGPPRVEHLVVLEGGP-VMEYISKSLDLPPLYVADLIHFG 133
           +SS+  G+ +Y+RL+PCP+ N PPR+EH+VVLE    V E+ISK LDLPPLYVADLI FG
Sbjct: 69  SSSSNSGYHEYNRLMPCPAYNLPPRIEHMVVLEDDVLVSEFISKQLDLPPLYVADLIRFG 128

Query: 134 AVYYALVCPRPPPTATPEQIRLFKKFTEPSLLRGRASIKGKTVREAQKTFRITRVDEFME 193
           AV+YALVCP+PPPTATPE+I LF++ T PS+L+ R+SIKGKTVREAQKTFR+T  +++ E
Sbjct: 129 AVHYALVCPKPPPTATPEEIILFEEVTSPSVLKKRSSIKGKTVREAQKTFRVTHTNQYAE 188

Query: 194 VGTYLRVHVHPKRFPRCYEIDWKSRIIAVRESYVVLDKPAGTSVGGTTDNIEESCATFAT 253
            GTYLRVHVHPKR PRCYEIDWKSRI+AV +SYV+LDKPAGT+VGGTTDNIEESCATFA+
Sbjct: 189 AGTYLRVHVHPKRSPRCYEIDWKSRIVAVTDSYVILDKPAGTTVGGTTDNIEESCATFAS 248

Query: 254 RALGLTSPLWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLALAAAPVPIGI 313
           RAL L  PL TTHQIDNCTEGC++ ARTKEYCSVFH KIR K+VKKLY ALAAAP+PIGI
Sbjct: 249 RALDLPEPLKTTHQIDNCTEGCVVFARTKEYCSVFHTKIRNKEVKKLYRALAAAPLPIGI 308

Query: 314 ITHYMRPINMAPRLVSEDCIDGWNLCQLEVLECREVPWPVADIEAKYSIEECGWPSKKKA 373
           I+HYMRP NMAPRLV+ED I GW+LCQLEVLEC+++PWP A  E K+ IE+CGW SK+ A
Sbjct: 309 ISHYMRPKNMAPRLVAEDSIQGWHLCQLEVLECKKIPWPDAATEKKHDIEDCGWTSKEFA 368

Query: 374 YECKINLLTGRTHQIRAQLADCGAPLVGDSMYMPAAVAETTNPGLNPFGKCQKQYTSEED 433
           YEC INLLTG+THQIRAQLA CGAPLVGDSMYMPAA+AE  NP  NP+GK +K YT EE+
Sbjct: 369 YECTINLLTGKTHQIRAQLAACGAPLVGDSMYMPAAIAEMGNPDTNPYGKAKKHYTMEEN 428

Query: 434 -KEMDVTEWIARHGKEPAVAIGLQAYEISW--DAGEHVYNAGIPWWK 473
            KE  V EWI RHGKEP V IGLQA +ISW  D GEH Y AG PWW+
Sbjct: 429 QKETAVAEWIDRHGKEPRVGIGLQACQISWDDDDGEHFYEAGTPWWR 472

[Close]
BLAST of MC03g0867 vs. TAIR 10
Match: AT5G51140.1 (Pseudouridine synthase family protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.9e-04
Identity = 38/155 (24.52%), Postives = 58/155 (37.42%), Query Frame = 0 

Query: 257 LWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLA--LAAAPVPIGIITHYMR 316
           L+  H++D    G +I+ART      F ++I    VKK Y+A  +   P    I+   + 
Sbjct: 180 LFPIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANIN 239

Query: 317 PINMAPRLVSEDC--------IDGWNLCQLEVLECREVPWPVADIEAKYSIEECGWPSKK 376
                 R  +ED         + G   C           +   D    +S+  C      
Sbjct: 240 YNGSEGRSTAEDANSSGDDKKVKGKPAC---------TKFTRIDTNGTHSLVSC------ 299

Query: 377 KAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYM 402
                    +TGRTHQIR  L   G P+  D +Y+
Sbjct: 300 -------EPVTGRTHQIRVHLQYTGHPIANDPLYL 312

[Close]
BLAST of MC03g0867 vs. TAIR 10
Match: AT5G51140.2 (Pseudouridine synthase family protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.9e-04
Identity = 38/155 (24.52%), Postives = 58/155 (37.42%), Query Frame = 0 

Query: 257 LWTTHQIDNCTEGCIILARTKEYCSVFHRKIREKKVKKLYLA--LAAAPVPIGIITHYMR 316
           L+  H++D    G +I+ART      F ++I    VKK Y+A  +   P    I+   + 
Sbjct: 180 LFPIHRLDRLVSGLLIIARTAAKADFFRQQIEGGMVKKRYIAKVIGVFPEDEMIVDANIN 239

Query: 317 PINMAPRLVSEDC--------IDGWNLCQLEVLECREVPWPVADIEAKYSIEECGWPSKK 376
                 R  +ED         + G   C           +   D    +S+  C      
Sbjct: 240 YNGSEGRSTAEDANSSGDDKKVKGKPAC---------TKFTRIDTNGTHSLVSC------ 299

Query: 377 KAYECKINLLTGRTHQIRAQLADCGAPLVGDSMYM 402
                    +TGRTHQIR  L   G P+  D +Y+
Sbjct: 300 -------EPVTGRTHQIRVHLQYTGHPIANDPLYL 312

The following BLAST results are available for this feature:
BLAST of MC03g0867 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SVS01.1e-18268.09RNA pseudouridine synthase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
A3BN262.5e-16364.68RNA pseudouridine synthase 6, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
P439307.2e-0924.23Uncharacterized RNA pseudouridine synthase HI_0042 OS=Haemophilus influenzae (st... [more]
P754851.0e-0725.70Uncharacterized RNA pseudouridine synthase MG209 homolog OS=Mycoplasma pneumonia... [more]
Q8PHN21.4e-0726.15Ribosomal large subunit pseudouridine synthase D OS=Xanthomonas axonopodis pv. c... [more]
BLAST of MC03g0867 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022139955.10.0100.00RNA pseudouridine synthase 6, chloroplastic isoform X1 [Momordica charantia][more]
XP_022994289.10.087.27RNA pseudouridine synthase 6, chloroplastic-like isoform X1 [Cucurbita maxima] >... [more]
XP_022954964.13.21e-31587.06RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita moschata] >XP_022954... [more]
KAG6573200.13.68e-31486.43RNA pseudouridine synthase 6, chloroplastic, partial [Cucurbita argyrosperma sub... [more]
XP_023542657.11.01e-31386.85RNA pseudouridine synthase 6, chloroplastic-like [Cucurbita pepo subsp. pepo] >X... [more]
BLAST of MC03g0867 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CDQ30.0100.00RNA pseudouridine synthase 6, chloroplastic isoform X1 OS=Momordica charantia OX... [more]
A0A6J1JVD90.087.27RNA pseudouridine synthase 6, chloroplastic-like isoform X1 OS=Cucurbita maxima ... [more]
A0A6J1GUJ21.55e-31587.06RNA pseudouridine synthase 6, chloroplastic-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1F4827.87e-30484.13RNA pseudouridine synthase 6, chloroplastic-like isoform X1 OS=Cucurbita moschat... [more]
A0A6J1KKP45.89e-29982.67RNA pseudouridine synthase 6, chloroplastic-like isoform X1 OS=Cucurbita maxima ... [more]
BLAST of MC03g0867 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G21770.17.8e-18468.09Pseudouridine synthase family protein [more]
AT5G51140.11.9e-0424.52Pseudouridine synthase family protein [more]
AT5G51140.21.9e-0424.52Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 221..388
e-value: 3.8E-23
score: 82.3
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 221..413
e-value: 2.19934E-33
score: 122.831
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 206..460
e-value: 4.2E-27
score: 97.1
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 19..471
NoneNo IPR availablePANTHERPTHR21600:SF52RNA PSEUDOURIDINE SYNTHASE 6, CHLOROPLASTICcoord: 19..471
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 206..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0867.1MC03g0867.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding