MC03g0827 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0827
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Aspergillus nuclease S(1)
Location	MC03: 14921591 .. 14925340 (-)
RNA-Seq Expression	MC03g0827
Synteny	MC03g0827

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AACACCTCTCTCACACTACAAATCATCTCTCTCTATATATTCAAAGCGATTGATCATCAAAGGGGGGAAAATGTGGCTGTTTAGACTATTTCTGGTTTTGCTGAGTTTTGCTTTCCTGGTTCTCCCCAGTGCTCAAGCATGGAGCAAAGAAGGCCACGTTCTAACATGCCAAATTGCACAGGTAACTTGAACAACAAACTTATAAAAATGGATAACATAATTCAATTCACTCGAATTAATATGCTCTCTGTTTTGTTTTAAATACAGGGCCTCCTGAATCCAGACGCAACAGAGGCCGTCCAAAATTTACTACCGGAAAATGTCGACGGAAACCTGTCGGCGTTGTGTGTTTGGCCGGACCAAATCCGACATTGGTATCGGTATCGCTGGACCAGTCCTCTTCACTTCATTGACACGCCCGATAATTCCTGTTCTTTCCTCTACACGAGTAACCATTTTTTCTTCTCTCTTTCATCAAACAATCTTGTCCATTTTTTATTCTTTGGATTTTTAATTGGTGGCTGTTTATAGGAGATTGCCACGACAACGGCGGCGTCGAGAACATGTGCGTCGCCGGCGCCGTTCGCAATTTCACCACTCAGCTCGCGCACTACGCAGAAGGGGGCTCCGATCGTCGACGTAATTAACCCGACCCGGCTCCGGTCCAAACCCGATTTGTTTTATTTTACTTAAACCCGATTATTTTTTTTGAAGACGACGATTTTTCATGGTTACTGCAGATAACTTGACCGGGGCGTTATTATTTCTATCGCACTTCCTTGGGGATATTCATCAGGTAAATAAAATATTGATTAAAATATTAAATAATCTAATTTAGTCCATTTAATTTGAAAAACTCCATTTCTCAAAAAAAAAAAAAAAAAAAAAATCCAATTTGATCTTTATTTGAGCTGAATTTTATGTTGTTCTCTGTAATTTAAAACCTCTTAATTTAGTATGCAGATCAAACTTTACAAATTATTTCCATTATACCATATTGAATACGAGTTAATGATTGGTATTTTATTTGAATTATTATATTAATTAATCGTGTCGGCTTAAAGATAAGTGAAATGTAAGTGTATTATCCAATAAATTTTTAGATATATCAGAACAAACTAAAATTTTTAAAATTATAGAGGCCACCCTTAAAAAAGTTTGAATAGCAAAAGAAATTTTATCCTCCGACTCCTCTATTTGATGAGATAGTCATTTTCCTTCTTAGTTTTTTTAATCTTTAACGAACAACTAATAATCAAAGGTAGTTTATGTAGTTTTTGAAAGAAAGAAGAGGTACATCGAAATTAATGAAACTAAAACTATAAGAAGTGAAAGGAGTAATTTAACCTAAAATGTCTATATCGATATGAAAAATTTTAATTAATTATCCGAGTGTAATTGTAAATTACAATTAACCCGGTGGTTTTACATCCAGCCAATGCACGTGGGGTTCACGAGCGACGAGGGAGGGAACACCATAAACTTACGGTGGTACCGCCACAAGTCCAATCTACACCACGTAAGCCCTAAGAAATAATTTAGACTTATAGCTTGAAATTAATTAATTATGACAATTCTTACTAATTAGGGAAAAAAAAAAAAACCACTGACGCAGGTGTGGGATAGGGAGATTATTCTCACAGCTCTAGCAGATTATTACGACAAGGACTCGGGCCTCCTCCTACAAGACATACAAAGGAATTTGACCCATGTAATTAATATAATTTATTTTACATTAATTAGGATAATATATATTAGTTTATAAGCTCATTTTTTAATACTAACTCAATTCTTGTAGGGAATTTGGACCCATGAGATTCCAACATGGCAGCATTGCGAAGATCTTGATTCATGCGTAAACAAGTATATGTTTATTTATATATTTGATCTCTCTATTTTAATTTTTTTCTCTCTAAATAATGCTTCATTTTTTCCTTTTTTTTAATGGAGGTGGGCTGAGGAGAGTATAAGCTTAGCTTGCAAGTGGGGTTATGAAGGAGTTGAGGCTGGTACAACTTTAGCAGGTAAATCTAATCAAACTTAAATTAATGGTCTTTCTTTAGTATAATAATAGGAAAGTAGATTTAAATTCACGATTTCTTGGTTGGTTCGGAATATATATGTCTGTTCAAATAGGCTAAATTAAGTTCCTTAATTATAACATAAATAATGAAGAATCAATAATCAAATTAAGTCCATTATTGGTTCTCTATTTATTTTAATAATGGATTAGTTATACTAAGTCTTCAATAATGAAGAATCAATATTCAAATTAGGCCCATTACCTGTCCTCTATTCATCTTAATAATGGGTTGGTTATACTGAAGTATTTTTTATATTTAGGGGCCTGCTTTAGATTATTTTGTTTTTAAAAAAACTGGATTTGTTTGTTAACATTTTATTTGTCTCACAGTCCATATTTAGAATAATTGTGAAAAATTATAAGAAATACTATAATGTTTTATGTTCACTTTTCAATTTGAAAAAAAGAAATTAAGTTTATTACATTATTCAGTTTATCGCTTTTGTATTTCATTTTTCGGGAACATTTTTGAAGTTTAATCAAAATATTAAAACATAAACACATTTTAAAAACTATCTTGTCAAGTTTTAAAAGATCTAGATATGGCCAATTTGAAAACATTAAGAAAAGGAACAAAATGAATATAGTAAAGTATACATTTCCAATAAAAAACAACAGTATCTATCAGGAAAAATATGGAGCAGTTTTTCTAAAATAATTTGGGTAGCTAATTAAGAGATAATATTCTCTAAATTTTTTTTTAAAAAAAGCTAATTAAGAGATGATAAATCTCTAACCAATGTGATTTTTGGGCAGAGGATTACTTCGATTCAAGGATGCCAATAGTGATGAAACGCATTGCTGAAGGTGGAGTCCGGTTGGCCATGCTTTTGAACCGGGTTTTTTCTGATGGAGGAGGAGGATTCGCCTCCTCAACTTGATTATTAATCCGACCGCTACATTTAATATAATATTAGCCAACAAAAACTCTCTCTTCTGTTTTTATGTTTATAAAATTAAATTTCTTTCTTTATTTCTGTAACATAGTAGTAGCAAATGCAGTAGAATCAGTTTATTACGTTTAAATCGTAGATTTGAATTCTCTCCACTTAATTTGTAATGTTATATTGTAAAGAAGAAATCTAACGATCATTGACTAACAATCTTGTTGAATGAGAAATTTCGGCTAATTACTAAAGGTCACGTCACAAAATTAAAATTACATTTAGGACCAATGACAAATTTACACGTGTCCTAAGTTAATTTTTAAATCGACGAATCAAATTGTGTCACATGTCCCTCTTATGGTTGTCCGATGAAATCAACTAATTGTATCAAAACCAGACTGGGTCCAAGCACTATAAATAGAGGGGTCACCTCTATTTGAAAGAAGGAGAAGAAGGATTGGGAAAGATCAAGAAGCATTGGAGATTCAGAGCATTCGGAGCATTTACAACCGTTCAAAGTGCGAAGCATCCAGAACCAACAAGACTCGATGAACTGAAGACTCGAAGAACGGAAGACTCGAAGACTACGAGACTTCAAGAATTCTAAATCTTCTCTTCGCCAAGCAACCGGCCGAAGAACTTCACAAGCTACAAGCCGAAGACTTCATCAACCTACAAACTTTGCCAAGCATCAAGCTTCACAGCCGAAACTCTAAAGATTCTTCAATACGCTCGAATCTGAAGGTGCAAACTCATTGTGAGAACAAGCTCACAAGCCCT

mRNA sequence

AACACCTCTCTCACACTACAAATCATCTCTCTCTATATATTCAAAGCGATTGATCATCAAAGGGGGGAAAATGTGGCTGTTTAGACTATTTCTGGTTTTGCTGAGTTTTGCTTTCCTGGTTCTCCCCAGTGCTCAAGCATGGAGCAAAGAAGGCCACGTTCTAACATGCCAAATTGCACAGGGCCTCCTGAATCCAGACGCAACAGAGGCCGTCCAAAATTTACTACCGGAAAATGTCGACGGAAACCTGTCGGCGTTGTGTGTTTGGCCGGACCAAATCCGACATTGGTATCGGTATCGCTGGACCAGTCCTCTTCACTTCATTGACACGCCCGATAATTCCTGTTCTTTCCTCTACACGAGAGATTGCCACGACAACGGCGGCGTCGAGAACATGTGCGTCGCCGGCGCCGTTCGCAATTTCACCACTCAGCTCGCGCACTACGCAGAAGGGGGCTCCGATCGTCGACATAACTTGACCGGGGCGTTATTATTTCTATCGCACTTCCTTGGGGATATTCATCAGCCAATGCACGTGGGGTTCACGAGCGACGAGGGAGGGAACACCATAAACTTACGGTGGTACCGCCACAAGTCCAATCTACACCACGTGTGGGATAGGGAGATTATTCTCACAGCTCTAGCAGATTATTACGACAAGGACTCGGGCCTCCTCCTACAAGACATACAAAGGAATTTGACCCATGGAATTTGGACCCATGAGATTCCAACATGGCAGCATTGCGAAGATCTTGATTCATGCGTAAACAAGTGGGCTGAGGAGAGTATAAGCTTAGCTTGCAAGTGGGGTTATGAAGGAGTTGAGGCTGGTACAACTTTAGCAGAGGATTACTTCGATTCAAGGATGCCAATAGTGATGAAACGCATTGCTGAAGGTGGAGTCCGGTTGGCCATGCTTTTGAACCGGGTTTTTTCTGATGGAGGAGGAGGATTCGCCTCCTCAACTTGATTATTAATCCGACCGCTACATTTAATATAATATTAGCCAACAAAAACTCTCTCTTCTGTTTTTATGTTTATAAAATTAAATTTCTTTCTTTATTTCTGTAACATAGTAGTAGCAAATGCAGTAGAATCAGTTTATTACGTTTAAATCGTAGATTTGAATTCTCTCCACTTAATTTGTAATGTTATATTGTAAAGAAGAAATCTAACGATCATTGACTAACAATCTTGTTGAATGAGAAATTTCGGCTAATTACTAAAGGTCACGTCACAAAATTAAAATTACATTTAGGACCAATGACAAATTTACACGTGTCCTAAGTTAATTTTTAAATCGACGAATCAAATTGTGTCACATGTCCCTCTTATGGTTGTCCGATGAAATCAACTAATTGTATCAAAACCAGACTGGGTCCAAGCACTATAAATAGAGGGGTCACCTCTATTTGAAAGAAGGAGAAGAAGGATTGGGAAAGATCAAGAAGCATTGGAGATTCAGAGCATTCGGAGCATTTACAACCGTTCAAAGTGCGAAGCATCCAGAACCAACAAGACTCGATGAACTGAAGACTCGAAGAACGGAAGACTCGAAGACTACGAGACTTCAAGAATTCTAAATCTTCTCTTCGCCAAGCAACCGGCCGAAGAACTTCACAAGCTACAAGCCGAAGACTTCATCAACCTACAAACTTTGCCAAGCATCAAGCTTCACAGCCGAAACTCTAAAGATTCTTCAATACGCTCGAATCTGAAGGTGCAAACTCATTGTGAGAACAAGCTCACAAGCCCT

Coding sequence (CDS)

ATGTGGCTGTTTAGACTATTTCTGGTTTTGCTGAGTTTTGCTTTCCTGGTTCTCCCCAGTGCTCAAGCATGGAGCAAAGAAGGCCACGTTCTAACATGCCAAATTGCACAGGGCCTCCTGAATCCAGACGCAACAGAGGCCGTCCAAAATTTACTACCGGAAAATGTCGACGGAAACCTGTCGGCGTTGTGTGTTTGGCCGGACCAAATCCGACATTGGTATCGGTATCGCTGGACCAGTCCTCTTCACTTCATTGACACGCCCGATAATTCCTGTTCTTTCCTCTACACGAGAGATTGCCACGACAACGGCGGCGTCGAGAACATGTGCGTCGCCGGCGCCGTTCGCAATTTCACCACTCAGCTCGCGCACTACGCAGAAGGGGGCTCCGATCGTCGACATAACTTGACCGGGGCGTTATTATTTCTATCGCACTTCCTTGGGGATATTCATCAGCCAATGCACGTGGGGTTCACGAGCGACGAGGGAGGGAACACCATAAACTTACGGTGGTACCGCCACAAGTCCAATCTACACCACGTGTGGGATAGGGAGATTATTCTCACAGCTCTAGCAGATTATTACGACAAGGACTCGGGCCTCCTCCTACAAGACATACAAAGGAATTTGACCCATGGAATTTGGACCCATGAGATTCCAACATGGCAGCATTGCGAAGATCTTGATTCATGCGTAAACAAGTGGGCTGAGGAGAGTATAAGCTTAGCTTGCAAGTGGGGTTATGAAGGAGTTGAGGCTGGTACAACTTTAGCAGAGGATTACTTCGATTCAAGGATGCCAATAGTGATGAAACGCATTGCTGAAGGTGGAGTCCGGTTGGCCATGCTTTTGAACCGGGTTTTTTCTGATGGAGGAGGAGGATTCGCCTCCTCAACTTGA

Protein sequence

MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST

Homology

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BLAST of MC03g0827 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 5.7e-121
Identity = 190/286 (66.43%), Postives = 239/286 (83.57%), Query Frame = 0


Query: 5   RLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALC 64
           RL LVL       + S ++WSKEGH+LTC+IAQ LL       V+NLLP+ V G+LSALC
Sbjct: 10  RLILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALC 69

Query: 65  VWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAH 124
           VWPDQIRHWY+YRWTS LH+IDTPD +CS+ Y+RDCHD  G+++MCV GA++NFT+QL H
Sbjct: 70  VWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQH 129

Query: 125 YAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDR 184
           Y EG SDRR+N+T ALLFLSHF+GDIHQPMHVGFTSDEGGNTI+LRWY+HKSNLHHVWDR
Sbjct: 130 YGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDR 189

Query: 185 EIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLAC 244
           EIILTAL + YDK+  LL +D+++N+T+G+W  ++ +W  C DL +C +K+A ESI LAC
Sbjct: 190 EIILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLAC 249

Query: 245 KWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSD 291
           KWGY+GV++G TL+E+YF++R+PIVMKRI +GGVRLAM+LNRVFSD
Sbjct: 250 KWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSD 295

[Close]

BLAST of MC03g0827 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 4.9e-88
Identity = 142/275 (51.64%), Postives = 197/275 (71.64%), Query Frame = 0


Query: 17  VLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRY 76
           ++  A  W KEGH   C+IA+     +   AV+ LLP++ DG+L+++C WPD+I+H +++
Sbjct: 18  LINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQW 77

Query: 77  RWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-N 136
           RWTSPLH++DTPD  C++ Y RDCHD    ++ CV GA+ N+T QL   +E      H N
Sbjct: 78  RWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYN 137

Query: 137 LTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYY 196
           LT AL+FLSHF+GDIHQP+HVGF  DEGGNTI +RWYR K+NLHHVWD  II +AL  YY
Sbjct: 138 LTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYY 197

Query: 197 DKDSGLLLQDIQRNLTHGIWTHEIPTWQHCE-DLDSCVNKWAEESISLACKWGYEGVEAG 256
           +K   L+++ +Q NLT+  W++++P W+ C+ +  +C N +A ESI+LACK+ Y     G
Sbjct: 198 NKSLPLMIEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPG 257

Query: 257 TTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFS 290
           TTL +DYF SR+PIV KR+A+GG+RLA  LNR+FS
Sbjct: 258 TTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]

BLAST of MC03g0827 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 2.2e-80
Identity = 134/284 (47.18%), Postives = 194/284 (68.31%), Query Frame = 0


Query: 6   LFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCV 65
           + +++  +     P+   W KEGH + C+IAQ  L+  A +AV+ LLPE+ +G+LS+LC+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 66  WPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHY 125
           W D+++  +RY W+SPLH+I+TPD +CS+ Y RDC D  G +  CVAGA+ N+TTQL  Y
Sbjct: 70  WADRVK--FRYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSY 129

Query: 126 AEGGSDR-RHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDR 185
               S + ++NLT ALLF+SHF+GDIHQP+HV + SD+GGNTI + WY  K+NLHH+WD 
Sbjct: 130 KTAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDS 189

Query: 186 EIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLAC 245
            II TA AD Y+     ++  +++N+T   W  ++  W+ C    +C + +A E I  AC
Sbjct: 190 NIIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAAC 249

Query: 246 KWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVF 289
            W Y+GV  G TL ++YF SR+PIV +R+A+GGVRLA  LNR+F
Sbjct: 250 DWAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of MC03g0827 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 4.6e-78
Identity = 134/288 (46.53%), Postives = 192/288 (66.67%), Query Frame = 0


Query: 5   RLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVD-GNLSAL 64
           RL++V +     ++  A  W K+GH   C++A+G    D   AV+ LLPE+VD G L+  
Sbjct: 2   RLWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADF 61

Query: 65  CVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLA 124
           C WPD+I+   +++WTS LH+++TP+  C++ Y RDCHD    ++ CV GA+ N+T QL 
Sbjct: 62  CSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLM 121

Query: 125 HYAEGGSDRRH-NLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVW 184
             +E   +  H NLT ALLFLSH++GD+HQP+H GF  D GGNTI + WY +KSNLHHVW
Sbjct: 122 SASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVW 181

Query: 185 DREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCE-DLDSCVNKWAEESIS 244
           D  II +AL  YY+     ++Q +Q  L +G W++++P+W+ C     +C N +A ESI 
Sbjct: 182 DNMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESID 241

Query: 245 LACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFS 290
           LACK+ Y     GTTL ++YF SR+P+V KR+A+GG+RLA  LNR+FS
Sbjct: 242 LACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]

BLAST of MC03g0827 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.6e-75
Identity = 131/290 (45.17%), Postives = 187/290 (64.48%), Query Frame = 0


Query: 2   WLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLS 61
           W  R+++V +     ++  A  W   GH   C+IAQ     D   AV+ LLPE+ +G L+
Sbjct: 3   WSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELA 62

Query: 62  ALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQ 121
           A+C WPD+I+   ++RWTS LHF DTPD  C++ Y+RDC      ++ CV GA+ N+T Q
Sbjct: 63  AVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQ 122

Query: 122 LAHYAEGGSDRRH-NLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 181
           L   +E      H NLT AL+FLSH++GDIHQP+H GF  D GGN I + WY  ++NLH 
Sbjct: 123 LMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHR 182

Query: 182 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCE-DLDSCVNKWAEES 241
           VWD  II +AL  YY+     ++ ++Q  L +G W++++P+W+ C+ +  +C N +A ES
Sbjct: 183 VWDDMIIESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASES 242

Query: 242 ISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFS 290
           I LACK+ Y    AGTTL + YF SR+P+V KR+A+GG+RLA  LNR+FS
Sbjct: 243 IDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

[Close]

BLAST of MC03g0827 vs. NCBI nr
Match: XP_022140160.1 (endonuclease 1 [Momordica charantia])

HSP 1 Score: 637 bits (1642), Expect = 1.04e-229
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0


Query: 1   MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL 60
           MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL
Sbjct: 75  MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL 134

Query: 61  SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT 120
           SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT
Sbjct: 135 SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT 194

Query: 121 QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 180
           QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH
Sbjct: 195 QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 254

Query: 181 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI 240
           VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI
Sbjct: 255 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI 314

Query: 241 SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST
Sbjct: 315 SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 373

[Close]

BLAST of MC03g0827 vs. NCBI nr
Match: XP_020221420.1 (endonuclease 1 [Cajanus cajan] >KYP63013.1 Nuclease PA3 [Cajanus cajan])

HSP 1 Score: 498 bits (1283), Expect = 4.75e-176
Identity = 221/293 (75.43%), Postives = 260/293 (88.74%), Query Frame = 0


Query: 7   FLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVW 66
           FL+  + AF+ +P A AWSKEGHV+TCQIAQ LL P+A EAV +LLPE V+GNLSALC+W
Sbjct: 16  FLLFFTSAFIGVPGALAWSKEGHVMTCQIAQALLEPEAAEAVHHLLPEYVNGNLSALCIW 75

Query: 67  PDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYA 126
           PDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+RDCHD  GVE+MCVAGAV+NFT+QL HY+
Sbjct: 76  PDQIRHWYKYRWTSPLHFIDTPDNACSFKYSRDCHDPHGVEDMCVAGAVKNFTSQLMHYS 135

Query: 127 EGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREI 186
           EG SDRRHN+T ALLFLSHF+GDIHQPMHVGFT+DEGGNTINLRW+RHKSNLHHVWDREI
Sbjct: 136 EGTSDRRHNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTINLRWFRHKSNLHHVWDREI 195

Query: 187 ILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKW 246
           ILTALADYYDKD  LLLQDI+RN T GIW+ ++ +W++C D+  CVN WA+ESI +ACKW
Sbjct: 196 ILTALADYYDKDVSLLLQDIERNYTDGIWSDDVTSWKNCNDISQCVNNWAKESIQIACKW 255

Query: 247 GYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           GYEGVE+GTTLA+DYFDSRMP VMKRIA+GG+RLAM+LN+VF+D   G A++T
Sbjct: 256 GYEGVESGTTLADDYFDSRMPYVMKRIAQGGIRLAMILNKVFADSEEGLATAT 308

[Close]

BLAST of MC03g0827 vs. NCBI nr
Match: XP_023000814.1 (endonuclease 1 [Cucurbita maxima])

HSP 1 Score: 494 bits (1273), Expect = 1.14e-174
Identity = 229/299 (76.59%), Postives = 261/299 (87.29%), Query Frame = 0


Query: 1   MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL 60
           MWLFR FLVLL F FL+LPSAQ WSKEGHVLTCQIAQ LLNP+ATEAVQ LLPE+  GNL
Sbjct: 1   MWLFR-FLVLLCFTFLLLPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNL 60

Query: 61  SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT 120
           SA+CVW DQIR W +YRWTSPLH+I+TPDN+CSFLY RDCH+     NMCVAGA+RNFTT
Sbjct: 61  SAMCVWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTT 120

Query: 121 QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 180
           QL  + + G D ++NLT ALLFLSHF+GDIHQP+HVGFTSDEGGNTI LRWYRHKSNLHH
Sbjct: 121 QLTAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHH 180

Query: 181 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI 240
           VWDREIILTALADYYDKD+GLLL+D+QRNLTHGIW+  +PTW+ C +++SC+N WAEESI
Sbjct: 181 VWDREIILTALADYYDKDTGLLLEDLQRNLTHGIWSDNVPTWERCVNVNSCINNWAEESI 240

Query: 241 SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGG-GGFASS 298
            LAC W YEGVEAG TL+EDYFDSR+PIVM+R+A+GGVRLAMLLNRVFS+   GGF SS
Sbjct: 241 KLACTWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFGSS 298

[Close]

BLAST of MC03g0827 vs. NCBI nr
Match: NP_001235325.1 (endonuclease [Glycine max] >ABP68856.1 endonuclease [Glycine max])

HSP 1 Score: 493 bits (1269), Expect = 6.44e-174
Identity = 218/293 (74.40%), Postives = 256/293 (87.37%), Query Frame = 0


Query: 7   FLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVW 66
           FL+  S AF+ +P A AWSKEGHV+TCQIAQ LL P+A+EAV  LLP++V GNLSALC W
Sbjct: 16  FLLFFSSAFIGVPGALAWSKEGHVMTCQIAQALLEPEASEAVYQLLPDHVKGNLSALCTW 75

Query: 67  PDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYA 126
           PDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+RDCHD  GVE+MCVAGAV+NFT+QL HY 
Sbjct: 76  PDQIRHWYKYRWTSPLHFIDTPDNACSFQYSRDCHDPQGVEDMCVAGAVKNFTSQLMHYK 135

Query: 127 EGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREI 186
           EG SDRR+N+T ALLFLSHF+GDIHQPMHVGFT+DEGGNTI LRW+RHKSNLHHVWDREI
Sbjct: 136 EGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDREI 195

Query: 187 ILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKW 246
           ILT LADYYDKD   LLQDI+RN T GIW+ ++ +W+HC D+  CVN WA+ESI +ACKW
Sbjct: 196 ILTGLADYYDKDVSFLLQDIERNYTDGIWSDDVTSWKHCNDISQCVNNWAKESIQIACKW 255

Query: 247 GYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           GYEGV+ GTTLA+DYFDSRMP+VMKRIA+GG+RLAM+LN+VF+D   GFA++T
Sbjct: 256 GYEGVQPGTTLADDYFDSRMPLVMKRIAQGGIRLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MC03g0827 vs. NCBI nr
Match: XP_028203080.1 (endonuclease 1-like [Glycine soja] >KAG4948408.1 hypothetical protein JHK86_041647 [Glycine max] >KAG4945541.1 hypothetical protein JHK87_041548 [Glycine soja] >KAG5104622.1 hypothetical protein JHK82_041592 [Glycine max] >KAG5115746.1 hypothetical protein JHK84_041859 [Glycine max] >KRH10768.1 hypothetical protein GLYMA_15G068600v4 [Glycine max])

HSP 1 Score: 492 bits (1267), Expect = 1.30e-173
Identity = 218/293 (74.40%), Postives = 255/293 (87.03%), Query Frame = 0


Query: 7   FLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVW 66
           FL+  S AF+ +P A AWSKEGHV+TCQIAQ LL P+A+EAV  LLP++V GNLSALC W
Sbjct: 16  FLLFFSSAFIGVPGALAWSKEGHVMTCQIAQALLEPEASEAVYQLLPDHVKGNLSALCTW 75

Query: 67  PDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYA 126
           PDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+RDCHD  GVE+MCVAGAV+NFT+QL HY 
Sbjct: 76  PDQIRHWYKYRWTSPLHFIDTPDNACSFQYSRDCHDPQGVEDMCVAGAVKNFTSQLMHYK 135

Query: 127 EGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREI 186
           EG SDRR+N+T ALLFLSHF+GDIHQPMHVGFT+DEGGNTI LRW+RHKSNLHHVWDREI
Sbjct: 136 EGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDREI 195

Query: 187 ILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKW 246
           ILT LADYYDKD   LLQDI+RN T GIW+ ++ +W+HC D+  CVN WA+ESI +ACKW
Sbjct: 196 ILTGLADYYDKDVSFLLQDIERNYTDGIWSDDVTSWKHCNDISQCVNNWAKESIQIACKW 255

Query: 247 GYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           GYEGV+ GTTLA+DYFDSRMP VMKRIA+GG+RLAM+LN+VF+D   GFA++T
Sbjct: 256 GYEGVQPGTTLADDYFDSRMPFVMKRIAQGGIRLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MC03g0827 vs. ExPASy TrEMBL
Match: A0A6J1CEC2 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111010892 PE=3 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 5.01e-230
Identity = 299/299 (100.00%), Postives = 299/299 (100.00%), Query Frame = 0


Query: 1   MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL 60
           MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL
Sbjct: 75  MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL 134

Query: 61  SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT 120
           SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT
Sbjct: 135 SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT 194

Query: 121 QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 180
           QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH
Sbjct: 195 QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 254

Query: 181 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI 240
           VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI
Sbjct: 255 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI 314

Query: 241 SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST
Sbjct: 315 SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 373

[Close]

BLAST of MC03g0827 vs. ExPASy TrEMBL
Match: A0A151T7J6 (Aspergillus nuclease S(1) OS=Cajanus cajan OX=3821 GN=KK1_017575 PE=3 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 2.30e-176
Identity = 221/293 (75.43%), Postives = 260/293 (88.74%), Query Frame = 0


Query: 7   FLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVW 66
           FL+  + AF+ +P A AWSKEGHV+TCQIAQ LL P+A EAV +LLPE V+GNLSALC+W
Sbjct: 16  FLLFFTSAFIGVPGALAWSKEGHVMTCQIAQALLEPEAAEAVHHLLPEYVNGNLSALCIW 75

Query: 67  PDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYA 126
           PDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+RDCHD  GVE+MCVAGAV+NFT+QL HY+
Sbjct: 76  PDQIRHWYKYRWTSPLHFIDTPDNACSFKYSRDCHDPHGVEDMCVAGAVKNFTSQLMHYS 135

Query: 127 EGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREI 186
           EG SDRRHN+T ALLFLSHF+GDIHQPMHVGFT+DEGGNTINLRW+RHKSNLHHVWDREI
Sbjct: 136 EGTSDRRHNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTINLRWFRHKSNLHHVWDREI 195

Query: 187 ILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKW 246
           ILTALADYYDKD  LLLQDI+RN T GIW+ ++ +W++C D+  CVN WA+ESI +ACKW
Sbjct: 196 ILTALADYYDKDVSLLLQDIERNYTDGIWSDDVTSWKNCNDISQCVNNWAKESIQIACKW 255

Query: 247 GYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           GYEGVE+GTTLA+DYFDSRMP VMKRIA+GG+RLAM+LN+VF+D   G A++T
Sbjct: 256 GYEGVESGTTLADDYFDSRMPYVMKRIAQGGIRLAMILNKVFADSEEGLATAT 308

[Close]

BLAST of MC03g0827 vs. ExPASy TrEMBL
Match: A0A6J1KL07 (Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111495151 PE=3 SV=1)

HSP 1 Score: 494 bits (1273), Expect = 5.53e-175
Identity = 229/299 (76.59%), Postives = 261/299 (87.29%), Query Frame = 0


Query: 1   MWLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNL 60
           MWLFR FLVLL F FL+LPSAQ WSKEGHVLTCQIAQ LLNP+ATEAVQ LLPE+  GNL
Sbjct: 1   MWLFR-FLVLLCFTFLLLPSAQGWSKEGHVLTCQIAQELLNPEATEAVQALLPESAGGNL 60

Query: 61  SALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTT 120
           SA+CVW DQIR W +YRWTSPLH+I+TPDN+CSFLY RDCH+     NMCVAGA+RNFTT
Sbjct: 61  SAMCVWADQIRRWSKYRWTSPLHYINTPDNACSFLYKRDCHNTAAQLNMCVAGAIRNFTT 120

Query: 121 QLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 180
           QL  + + G D ++NLT ALLFLSHF+GDIHQP+HVGFTSDEGGNTI LRWYRHKSNLHH
Sbjct: 121 QLTAFPKQGPDAKNNLTEALLFLSHFVGDIHQPLHVGFTSDEGGNTIELRWYRHKSNLHH 180

Query: 181 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESI 240
           VWDREIILTALADYYDKD+GLLL+D+QRNLTHGIW+  +PTW+ C +++SC+N WAEESI
Sbjct: 181 VWDREIILTALADYYDKDTGLLLEDLQRNLTHGIWSDNVPTWERCVNVNSCINNWAEESI 240

Query: 241 SLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGG-GGFASS 298
            LAC W YEGVEAG TL+EDYFDSR+PIVM+R+A+GGVRLAMLLNRVFS+   GGF SS
Sbjct: 241 KLACTWAYEGVEAGMTLSEDYFDSRLPIVMERLAKGGVRLAMLLNRVFSENPKGGFGSS 298

[Close]

BLAST of MC03g0827 vs. ExPASy TrEMBL
Match: A5A338 (Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=EN1 PE=2 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 3.12e-174
Identity = 218/293 (74.40%), Postives = 256/293 (87.37%), Query Frame = 0


Query: 7   FLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVW 66
           FL+  S AF+ +P A AWSKEGHV+TCQIAQ LL P+A+EAV  LLP++V GNLSALC W
Sbjct: 16  FLLFFSSAFIGVPGALAWSKEGHVMTCQIAQALLEPEASEAVYQLLPDHVKGNLSALCTW 75

Query: 67  PDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYA 126
           PDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+RDCHD  GVE+MCVAGAV+NFT+QL HY 
Sbjct: 76  PDQIRHWYKYRWTSPLHFIDTPDNACSFQYSRDCHDPQGVEDMCVAGAVKNFTSQLMHYK 135

Query: 127 EGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREI 186
           EG SDRR+N+T ALLFLSHF+GDIHQPMHVGFT+DEGGNTI LRW+RHKSNLHHVWDREI
Sbjct: 136 EGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDREI 195

Query: 187 ILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKW 246
           ILT LADYYDKD   LLQDI+RN T GIW+ ++ +W+HC D+  CVN WA+ESI +ACKW
Sbjct: 196 ILTGLADYYDKDVSFLLQDIERNYTDGIWSDDVTSWKHCNDISQCVNNWAKESIQIACKW 255

Query: 247 GYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           GYEGV+ GTTLA+DYFDSRMP+VMKRIA+GG+RLAM+LN+VF+D   GFA++T
Sbjct: 256 GYEGVQPGTTLADDYFDSRMPLVMKRIAQGGIRLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MC03g0827 vs. ExPASy TrEMBL
Match: I1MEB5 (Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=GLYMA_15G068600 PE=3 SV=1)

HSP 1 Score: 492 bits (1267), Expect = 6.29e-174
Identity = 218/293 (74.40%), Postives = 255/293 (87.03%), Query Frame = 0


Query: 7   FLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVW 66
           FL+  S AF+ +P A AWSKEGHV+TCQIAQ LL P+A+EAV  LLP++V GNLSALC W
Sbjct: 16  FLLFFSSAFIGVPGALAWSKEGHVMTCQIAQALLEPEASEAVYQLLPDHVKGNLSALCTW 75

Query: 67  PDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYA 126
           PDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+RDCHD  GVE+MCVAGAV+NFT+QL HY 
Sbjct: 76  PDQIRHWYKYRWTSPLHFIDTPDNACSFQYSRDCHDPQGVEDMCVAGAVKNFTSQLMHYK 135

Query: 127 EGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREI 186
           EG SDRR+N+T ALLFLSHF+GDIHQPMHVGFT+DEGGNTI LRW+RHKSNLHHVWDREI
Sbjct: 136 EGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDREI 195

Query: 187 ILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKW 246
           ILT LADYYDKD   LLQDI+RN T GIW+ ++ +W+HC D+  CVN WA+ESI +ACKW
Sbjct: 196 ILTGLADYYDKDVSFLLQDIERNYTDGIWSDDVTSWKHCNDISQCVNNWAKESIQIACKW 255

Query: 247 GYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST 299
           GYEGV+ GTTLA+DYFDSRMP VMKRIA+GG+RLAM+LN+VF+D   GFA++T
Sbjct: 256 GYEGVQPGTTLADDYFDSRMPFVMKRIAQGGIRLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MC03g0827 vs. TAIR 10
Match: AT1G11190.1 (bifunctional nuclease i )

HSP 1 Score: 435.3 bits (1118), Expect = 4.1e-122
Identity = 190/286 (66.43%), Postives = 239/286 (83.57%), Query Frame = 0


Query: 5   RLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALC 64
           RL LVL       + S ++WSKEGH+LTC+IAQ LL       V+NLLP+ V G+LSALC
Sbjct: 10  RLILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALC 69

Query: 65  VWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAH 124
           VWPDQIRHWY+YRWTS LH+IDTPD +CS+ Y+RDCHD  G+++MCV GA++NFT+QL H
Sbjct: 70  VWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQH 129

Query: 125 YAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDR 184
           Y EG SDRR+N+T ALLFLSHF+GDIHQPMHVGFTSDEGGNTI+LRWY+HKSNLHHVWDR
Sbjct: 130 YGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDR 189

Query: 185 EIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLAC 244
           EIILTAL + YDK+  LL +D+++N+T+G+W  ++ +W  C DL +C +K+A ESI LAC
Sbjct: 190 EIILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLAC 249

Query: 245 KWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSD 291
           KWGY+GV++G TL+E+YF++R+PIVMKRI +GGVRLAM+LNRVFSD
Sbjct: 250 KWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSD 295

[Close]

BLAST of MC03g0827 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 325.9 bits (834), Expect = 3.5e-89
Identity = 142/275 (51.64%), Postives = 197/275 (71.64%), Query Frame = 0


Query: 17  VLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRY 76
           ++  A  W KEGH   C+IA+     +   AV+ LLP++ DG+L+++C WPD+I+H +++
Sbjct: 18  LINGALCWGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQW 77

Query: 77  RWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-N 136
           RWTSPLH++DTPD  C++ Y RDCHD    ++ CV GA+ N+T QL   +E      H N
Sbjct: 78  RWTSPLHYVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYN 137

Query: 137 LTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYY 196
           LT AL+FLSHF+GDIHQP+HVGF  DEGGNTI +RWYR K+NLHHVWD  II +AL  YY
Sbjct: 138 LTEALMFLSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYY 197

Query: 197 DKDSGLLLQDIQRNLTHGIWTHEIPTWQHCE-DLDSCVNKWAEESISLACKWGYEGVEAG 256
           +K   L+++ +Q NLT+  W++++P W+ C+ +  +C N +A ESI+LACK+ Y     G
Sbjct: 198 NKSLPLMIEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPG 257

Query: 257 TTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFS 290
           TTL +DYF SR+PIV KR+A+GG+RLA  LNR+FS
Sbjct: 258 TTLGDDYFLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]

BLAST of MC03g0827 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 134/284 (47.18%), Postives = 194/284 (68.31%), Query Frame = 0


Query: 6   LFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLSALCV 65
           + +++  +     P+   W KEGH + C+IAQ  L+  A +AV+ LLPE+ +G+LS+LC+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 66  WPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHY 125
           W D+++  +RY W+SPLH+I+TPD +CS+ Y RDC D  G +  CVAGA+ N+TTQL  Y
Sbjct: 70  WADRVK--FRYHWSSPLHYINTPD-ACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSY 129

Query: 126 AEGGSDR-RHNLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDR 185
               S + ++NLT ALLF+SHF+GDIHQP+HV + SD+GGNTI + WY  K+NLHH+WD 
Sbjct: 130 KTAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDS 189

Query: 186 EIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLAC 245
            II TA AD Y+     ++  +++N+T   W  ++  W+ C    +C + +A E I  AC
Sbjct: 190 NIIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAAC 249

Query: 246 KWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVF 289
            W Y+GV  G TL ++YF SR+PIV +R+A+GGVRLA  LNR+F
Sbjct: 250 DWAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]

BLAST of MC03g0827 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 292.7 bits (748), Expect = 3.3e-79
Identity = 134/288 (46.53%), Postives = 192/288 (66.67%), Query Frame = 0


Query: 5   RLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVD-GNLSAL 64
           RL++V +     ++  A  W K+GH   C++A+G    D   AV+ LLPE+VD G L+  
Sbjct: 2   RLWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADF 61

Query: 65  CVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLA 124
           C WPD+I+   +++WTS LH+++TP+  C++ Y RDCHD    ++ CV GA+ N+T QL 
Sbjct: 62  CSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLM 121

Query: 125 HYAEGGSDRRH-NLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVW 184
             +E   +  H NLT ALLFLSH++GD+HQP+H GF  D GGNTI + WY +KSNLHHVW
Sbjct: 122 SASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVW 181

Query: 185 DREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCE-DLDSCVNKWAEESIS 244
           D  II +AL  YY+     ++Q +Q  L +G W++++P+W+ C     +C N +A ESI 
Sbjct: 182 DNMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESID 241

Query: 245 LACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFS 290
           LACK+ Y     GTTL ++YF SR+P+V KR+A+GG+RLA  LNR+FS
Sbjct: 242 LACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]

BLAST of MC03g0827 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 283.1 bits (723), Expect = 2.6e-76
Identity = 131/290 (45.17%), Postives = 187/290 (64.48%), Query Frame = 0


Query: 2   WLFRLFLVLLSFAFLVLPSAQAWSKEGHVLTCQIAQGLLNPDATEAVQNLLPENVDGNLS 61
           W  R+++V +     ++  A  W   GH   C+IAQ     D   AV+ LLPE+ +G L+
Sbjct: 3   WSLRMWIVSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELA 62

Query: 62  ALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQ 121
           A+C WPD+I+   ++RWTS LHF DTPD  C++ Y+RDC      ++ CV GA+ N+T Q
Sbjct: 63  AVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-----PKDWCVTGAIFNYTNQ 122

Query: 122 LAHYAEGGSDRRH-NLTGALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHH 181
           L   +E      H NLT AL+FLSH++GDIHQP+H GF  D GGN I + WY  ++NLH 
Sbjct: 123 LMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHR 182

Query: 182 VWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCE-DLDSCVNKWAEES 241
           VWD  II +AL  YY+     ++ ++Q  L +G W++++P+W+ C+ +  +C N +A ES
Sbjct: 183 VWDDMIIESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASES 242

Query: 242 ISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFS 290
           I LACK+ Y    AGTTL + YF SR+P+V KR+A+GG+RLA  LNR+FS
Sbjct: 243 IDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

The following BLAST results are available for this feature:

BLAST of MC03g0827 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXA6	5.7e-121	66.43	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]
F4JJL0	4.9e-88	51.64	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
Q9C9G4	2.2e-80	47.18	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
F4JJL3	4.6e-78	46.53	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	3.6e-75	45.17	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]

BLAST of MC03g0827 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140160.1	1.04e-229	100.00	endonuclease 1 [Momordica charantia]	[more]
XP_020221420.1	4.75e-176	75.43	endonuclease 1 [Cajanus cajan] >KYP63013.1 Nuclease PA3 [Cajanus cajan]	[more]
XP_023000814.1	1.14e-174	76.59	endonuclease 1 [Cucurbita maxima]	[more]
NP_001235325.1	6.44e-174	74.40	endonuclease [Glycine max] >ABP68856.1 endonuclease [Glycine max]	[more]
XP_028203080.1	1.30e-173	74.40	endonuclease 1-like [Glycine soja] >KAG4948408.1 hypothetical protein JHK86_0416...	[more]

BLAST of MC03g0827 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEC2	5.01e-230	100.00	Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111010892 PE=3 SV...	[more]
A0A151T7J6	2.30e-176	75.43	Aspergillus nuclease S(1) OS=Cajanus cajan OX=3821 GN=KK1_017575 PE=3 SV=1	[more]
A0A6J1KL07	5.53e-175	76.59	Aspergillus nuclease S(1) OS=Cucurbita maxima OX=3661 GN=LOC111495151 PE=3 SV=1	[more]
A5A338	3.12e-174	74.40	Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=EN1 PE=2 SV=1	[more]
I1MEB5	6.29e-174	74.40	Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=GLYMA_15G068600 PE=3 SV=1	[more]

BLAST of MC03g0827 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11190.1	4.1e-122	66.43	bifunctional nuclease i	[more]
AT4G21585.1	3.5e-89	51.64	endonuclease 4	[more]
AT1G68290.1	1.6e-81	47.18	endonuclease 2	[more]
AT4G21600.1	3.3e-79	46.53	endonuclease 5	[more]
AT4G21590.1	2.6e-76	45.17	endonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 24..292 e-value: 5.3E-98 score: 330.1
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 24..286
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 24..288 e-value: 3.5E-79 score: 266.2
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 6..291
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 24..288 e-value: 2.01008E-81 score: 244.602
None	No IPR available	PANTHER	PTHR33146:SF14	ENDONUCLEASE 1	coord: 6..291

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0827.1	MC03g0827.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006413	translational initiation
cellular_component	GO:0005852	eukaryotic translation initiation factor 3 complex
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004521	endoribonuclease activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0000014	single-stranded DNA endodeoxyribonuclease activity
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0003676	nucleic acid binding