MC03g0733 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0733
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationMC03: 14120268 .. 14122406 (-)
RNA-Seq ExpressionMC03g0733
SyntenyMC03g0733
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCGCTGCGATTTTCTCGTCTTTACGAAGGAGGAGATCGCCGTCGCTGGAGGCTTTCTTGGCCCCCGTTGACCTCTCCGATGTGGCCTTAGTTCAAACCCTAGCGTTAGTTGCGACCGAGCTCGTTTCGCGATTTTCAGACAAGGCCTTCTTCTTCCAGCGCCGGAATTCGCGATCTCTGATACGGAGGCTCCAGGTTTGCCTCGTCTTTTTGGAATTTTTGAAGGATTCGGATGCCACTTTGCCTTACACCGCCTTGCTCTGTTTGAAAGAGCTTTATTTATTACTCTACCGCTCCAAGATACTTCTTGATTATTGCTCCGAATCGAGCAAGTTGTGGCTCTTGCTTCAGAACCACTCCATCTCCGGCCATTTCAATGATTTGAATTTAGAGTTGTTGACCTTTTTGGATGTGTTCCCTCTTGAGGAAGTTGAATTGGGGGTGGACATTAGAGAGCAGGTGGAGCTCTTGCAGAAGCAGTTGAAAAGGGCGAGGATATTTATTGATGAACGTGATGAAGCGTTGAGGATTCGCTTCCTTTTGTTTCTTGAGGAATTTGAGAACGGGCGGCTTCCCAATCCGAGAGAAATGAGGGAGTTTTTTGTGGACAAGTTGGGGATTCGGAATGCCAAGAGTTGCAGAGCTGAAATTGAGTTTCTAGAGGAGCAGATTGTCAATCATGAGGGCGACATCGAGCCCACGGTAGCAGTGCTATATGGTTTCGTTGCATTTACGAGATACAGCAGGTTTTTCCTCTTTGGGTTTGAGGAAGATGAAATCGAATCAGGTGCAACGAACCAGAAGAAGCTGAAGAAGAATTTGATAACACAGGAAATTGCCGAGACGTTTTTAACCATTCCAAGGGATTTCTGCTGTCCAATATCACTGGATTTGATGAAAGACCCAGTGATAGTTTCTACAGGCCAGACATACGATCGCAGTTCAATCGTCCGGTGGATGGAAGAAGGGCACTGCACTTGCCCGAAGACGGGGCAAATGCTCATCCATACCCGCCTCGCTCCCAACCGTGCTTTAAGGAATTTGATCATGCAGTGGTGCATAGCCCACGGAGTTCCTTATGATCCACCAGAGGGGATGGATCCATCTGCAGAAAGTTTTGCAACGGCTTCCCCCACTAGAGCTGCACTAGAAGCCAACAGAGCGACAGCTGCAATTCTTATCCAACAGTTAGCAATTGGATCACAAGATGCGCAGACCATCGCTGCTCGAGAGATTCGTTTGTTGGCGAAAACAGGGAAAGAGAACCGTGCTTTTATTGCAGAAGCCGGTGCCATCCCTCATCTCCAAAAGTTGCTGGCTTCCCCCAACCCCGTTGCACAGGAGAATTCGGTGACTGCTATGCTCAATCTCTCAATATATGACAAGAACAAAAGTCTGATTATGAGTGAGGTAGGGTGCTTAGGATCAATAACCGAAGTGTTGAGATTTGGACACACTACAGAAGCACGGGAAAATGCTGCAGCTACATTATTCAGCCTGTCTGCAGTTCATGACTTCAAGAAGAGAATAGCAGAGGAGGGTGGTGCAGTTGAAGCCTTGGCAGGTTTGTTGAGAAATGGTACACCAAGAGGAAAGAAGGATGCCGTAACAGCCTTATTTAATCTGTCAACCCACACCGATAACTGTGTGCGGATGATCGAGGCCGGGGCAGTAACAGCACTTGTAGGAGCATTGGGGAATGAAGGTGTTGCTGAGGAAGCAGCAGGTGCATTGGCCTTGATTGTTAGACAGCCCGTTGGTGCTGCAGCAGTGGGTAAAGAGGAGAGAGCCGTGGCAGGATTGATAGCAATGATGCGATGTGGGACGCCGAGAGGAAAAGAGAACGCAGTTGCAGCTTTGCTTGAGTTATGCCGAAGTGGGGGAGCTGCCACAACCGAGCAAGTATTCAAGGCCCCCGCTTTGGCTGGATTGCTCCAAACGCTTCTATTTACCGGTACCAAGCGAGCAAGAAGAAAGGCTGCTTCACTAGCCAGAGTGTTCCAAAGATGCGAGCATGTTGCAATGCATTATGGTGGATTGGGTGTTGGTTATGCCTATGCAACTAACTCGGCCTCGGATCCGAGCTTTACAAGCGAGGTGTCTGTGCCAATATCCATCTCGGTACCCGTCGTA

mRNA sequence

ATGGCTGCCGCTGCGATTTTCTCGTCTTTACGAAGGAGGAGATCGCCGTCGCTGGAGGCTTTCTTGGCCCCCGTTGACCTCTCCGATGTGGCCTTAGTTCAAACCCTAGCGTTAGTTGCGACCGAGCTCGTTTCGCGATTTTCAGACAAGGCCTTCTTCTTCCAGCGCCGGAATTCGCGATCTCTGATACGGAGGCTCCAGGTTTGCCTCGTCTTTTTGGAATTTTTGAAGGATTCGGATGCCACTTTGCCTTACACCGCCTTGCTCTGTTTGAAAGAGCTTTATTTATTACTCTACCGCTCCAAGATACTTCTTGATTATTGCTCCGAATCGAGCAAGTTGTGGCTCTTGCTTCAGAACCACTCCATCTCCGGCCATTTCAATGATTTGAATTTAGAGTTGTTGACCTTTTTGGATGTGTTCCCTCTTGAGGAAGTTGAATTGGGGGTGGACATTAGAGAGCAGGTGGAGCTCTTGCAGAAGCAGTTGAAAAGGGCGAGGATATTTATTGATGAACGTGATGAAGCGTTGAGGATTCGCTTCCTTTTGTTTCTTGAGGAATTTGAGAACGGGCGGCTTCCCAATCCGAGAGAAATGAGGGAGTTTTTTGTGGACAAGTTGGGGATTCGGAATGCCAAGAGTTGCAGAGCTGAAATTGAGTTTCTAGAGGAGCAGATTGTCAATCATGAGGGCGACATCGAGCCCACGGTAGCAGTGCTATATGGTTTCGTTGCATTTACGAGATACAGCAGGTTTTTCCTCTTTGGGTTTGAGGAAGATGAAATCGAATCAGGTGCAACGAACCAGAAGAAGCTGAAGAAGAATTTGATAACACAGGAAATTGCCGAGACGTTTTTAACCATTCCAAGGGATTTCTGCTGTCCAATATCACTGGATTTGATGAAAGACCCAGTGATAGTTTCTACAGGCCAGACATACGATCGCAGTTCAATCGTCCGGTGGATGGAAGAAGGGCACTGCACTTGCCCGAAGACGGGGCAAATGCTCATCCATACCCGCCTCGCTCCCAACCGTGCTTTAAGGAATTTGATCATGCAGTGGTGCATAGCCCACGGAGTTCCTTATGATCCACCAGAGGGGATGGATCCATCTGCAGAAAGTTTTGCAACGGCTTCCCCCACTAGAGCTGCACTAGAAGCCAACAGAGCGACAGCTGCAATTCTTATCCAACAGTTAGCAATTGGATCACAAGATGCGCAGACCATCGCTGCTCGAGAGATTCGTTTGTTGGCGAAAACAGGGAAAGAGAACCGTGCTTTTATTGCAGAAGCCGGTGCCATCCCTCATCTCCAAAAGTTGCTGGCTTCCCCCAACCCCGTTGCACAGGAGAATTCGGTGACTGCTATGCTCAATCTCTCAATATATGACAAGAACAAAAGTCTGATTATGAGTGAGGTAGGGTGCTTAGGATCAATAACCGAAGTGTTGAGATTTGGACACACTACAGAAGCACGGGAAAATGCTGCAGCTACATTATTCAGCCTGTCTGCAGTTCATGACTTCAAGAAGAGAATAGCAGAGGAGGGTGGTGCAGTTGAAGCCTTGGCAGGTTTGTTGAGAAATGGTACACCAAGAGGAAAGAAGGATGCCGTAACAGCCTTATTTAATCTGTCAACCCACACCGATAACTGTGTGCGGATGATCGAGGCCGGGGCAGTAACAGCACTTGTAGGAGCATTGGGGAATGAAGGTGTTGCTGAGGAAGCAGCAGGTGCATTGGCCTTGATTGTTAGACAGCCCGTTGGTGCTGCAGCAGTGGGTAAAGAGGAGAGAGCCGTGGCAGGATTGATAGCAATGATGCGATGTGGGACGCCGAGAGGAAAAGAGAACGCAGTTGCAGCTTTGCTTGAGTTATGCCGAAGTGGGGGAGCTGCCACAACCGAGCAAGTATTCAAGGCCCCCGCTTTGGCTGGATTGCTCCAAACGCTTCTATTTACCGGTACCAAGCGAGCAAGAAGAAAGGCTGCTTCACTAGCCAGAGTGTTCCAAAGATGCGAGCATGTTGCAATGCATTATGGTGGATTGGGTGTTGGTTATGCCTATGCAACTAACTCGGCCTCGGATCCGAGCTTTACAAGCGAGGTGTCTGTGCCAATATCCATCTCGGTACCCGTCGTA

Coding sequence (CDS)

ATGGCTGCCGCTGCGATTTTCTCGTCTTTACGAAGGAGGAGATCGCCGTCGCTGGAGGCTTTCTTGGCCCCCGTTGACCTCTCCGATGTGGCCTTAGTTCAAACCCTAGCGTTAGTTGCGACCGAGCTCGTTTCGCGATTTTCAGACAAGGCCTTCTTCTTCCAGCGCCGGAATTCGCGATCTCTGATACGGAGGCTCCAGGTTTGCCTCGTCTTTTTGGAATTTTTGAAGGATTCGGATGCCACTTTGCCTTACACCGCCTTGCTCTGTTTGAAAGAGCTTTATTTATTACTCTACCGCTCCAAGATACTTCTTGATTATTGCTCCGAATCGAGCAAGTTGTGGCTCTTGCTTCAGAACCACTCCATCTCCGGCCATTTCAATGATTTGAATTTAGAGTTGTTGACCTTTTTGGATGTGTTCCCTCTTGAGGAAGTTGAATTGGGGGTGGACATTAGAGAGCAGGTGGAGCTCTTGCAGAAGCAGTTGAAAAGGGCGAGGATATTTATTGATGAACGTGATGAAGCGTTGAGGATTCGCTTCCTTTTGTTTCTTGAGGAATTTGAGAACGGGCGGCTTCCCAATCCGAGAGAAATGAGGGAGTTTTTTGTGGACAAGTTGGGGATTCGGAATGCCAAGAGTTGCAGAGCTGAAATTGAGTTTCTAGAGGAGCAGATTGTCAATCATGAGGGCGACATCGAGCCCACGGTAGCAGTGCTATATGGTTTCGTTGCATTTACGAGATACAGCAGGTTTTTCCTCTTTGGGTTTGAGGAAGATGAAATCGAATCAGGTGCAACGAACCAGAAGAAGCTGAAGAAGAATTTGATAACACAGGAAATTGCCGAGACGTTTTTAACCATTCCAAGGGATTTCTGCTGTCCAATATCACTGGATTTGATGAAAGACCCAGTGATAGTTTCTACAGGCCAGACATACGATCGCAGTTCAATCGTCCGGTGGATGGAAGAAGGGCACTGCACTTGCCCGAAGACGGGGCAAATGCTCATCCATACCCGCCTCGCTCCCAACCGTGCTTTAAGGAATTTGATCATGCAGTGGTGCATAGCCCACGGAGTTCCTTATGATCCACCAGAGGGGATGGATCCATCTGCAGAAAGTTTTGCAACGGCTTCCCCCACTAGAGCTGCACTAGAAGCCAACAGAGCGACAGCTGCAATTCTTATCCAACAGTTAGCAATTGGATCACAAGATGCGCAGACCATCGCTGCTCGAGAGATTCGTTTGTTGGCGAAAACAGGGAAAGAGAACCGTGCTTTTATTGCAGAAGCCGGTGCCATCCCTCATCTCCAAAAGTTGCTGGCTTCCCCCAACCCCGTTGCACAGGAGAATTCGGTGACTGCTATGCTCAATCTCTCAATATATGACAAGAACAAAAGTCTGATTATGAGTGAGGTAGGGTGCTTAGGATCAATAACCGAAGTGTTGAGATTTGGACACACTACAGAAGCACGGGAAAATGCTGCAGCTACATTATTCAGCCTGTCTGCAGTTCATGACTTCAAGAAGAGAATAGCAGAGGAGGGTGGTGCAGTTGAAGCCTTGGCAGGTTTGTTGAGAAATGGTACACCAAGAGGAAAGAAGGATGCCGTAACAGCCTTATTTAATCTGTCAACCCACACCGATAACTGTGTGCGGATGATCGAGGCCGGGGCAGTAACAGCACTTGTAGGAGCATTGGGGAATGAAGGTGTTGCTGAGGAAGCAGCAGGTGCATTGGCCTTGATTGTTAGACAGCCCGTTGGTGCTGCAGCAGTGGGTAAAGAGGAGAGAGCCGTGGCAGGATTGATAGCAATGATGCGATGTGGGACGCCGAGAGGAAAAGAGAACGCAGTTGCAGCTTTGCTTGAGTTATGCCGAAGTGGGGGAGCTGCCACAACCGAGCAAGTATTCAAGGCCCCCGCTTTGGCTGGATTGCTCCAAACGCTTCTATTTACCGGTACCAAGCGAGCAAGAAGAAAGGCTGCTTCACTAGCCAGAGTGTTCCAAAGATGCGAGCATGTTGCAATGCATTATGGTGGATTGGGTGTTGGTTATGCCTATGCAACTAACTCGGCCTCGGATCCGAGCTTTACAAGCGAGGTGTCTGTGCCAATATCCATCTCGGTACCCGTCGTA

Protein sequence

MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSRSLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQNHSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIRFLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVLYGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGVPYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTALFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARRKAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV
Homology
BLAST of MC03g0733 vs. ExPASy Swiss-Prot
Match: Q9C7R6 (U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 5.3e-266
Identity = 498/729 (68.31%), Postives = 592/729 (81.21%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MA+AAIFSSLRRRRSPSLEAFLAPVDLS VALVQTLA +++E+VS F+   F FQR+N+R
Sbjct: 1   MASAAIFSSLRRRRSPSLEAFLAPVDLSGVALVQTLASISSEVVSCFTSVRFSFQRKNAR 60

Query: 61  SLIRRLQVCLVFLEFLKDSD----------------ATLPYTALLCLKELYLLLYRSKIL 120
           SLIR++++ +V  EFL DS+                +    TALLCLKELYLLLYRSKIL
Sbjct: 61  SLIRKIEIFVVLFEFLVDSNWGSTTTRTRARRRSKSSVSESTALLCLKELYLLLYRSKIL 120

Query: 121 LDYCSESSKLWLLLQNHSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLK 180
           +DYC++SSKLWLLLQN SISG+F+DLN E+ T LDV P+ ++ L  DIREQ+ELLQ+Q +
Sbjct: 121 VDYCAQSSKLWLLLQNPSISGYFHDLNQEISTLLDVLPVNDLGLSDDIREQIELLQRQSR 180

Query: 181 RARIFIDERDEALRIRFLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEE 240
           +AR++ID+ DE+LR  F  FL+ FENG++P+  ++R FFV+KLGIR++KSCR+EIEFLEE
Sbjct: 181 KARLYIDKNDESLRESFYSFLDGFENGKIPSSVDLRMFFVEKLGIRDSKSCRSEIEFLEE 240

Query: 241 QIVNHEGDIEPTVAVLYGFVAFTRYSRFFLFGFEEDEIESG-ATNQKKLKKNLITQEIAE 300
           QIVNH+GD+EPT +V+ GFVA TRY RF LFGFEED +E     N KK +K  + QEI +
Sbjct: 241 QIVNHDGDLEPTGSVINGFVAITRYCRFLLFGFEEDGMEWWIENNPKKPRKGFVAQEIGD 300

Query: 301 TFLTIPRDFCCPISLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAP 360
           TF+T+P+DF CPISLDLM DPVI+STGQTYDR+SI RW+EEGHCTCPKTGQML+ +R+ P
Sbjct: 301 TFITVPKDFVCPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVP 360

Query: 361 NRALRNLIMQWCIAHGVPYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGS 420
           NRAL+NLI+QWC A G+ Y+  E  D   ESFA+A PT+AA+EAN+AT +ILI+ LA GS
Sbjct: 361 NRALKNLIVQWCTASGISYE-SEFTDSPNESFASALPTKAAVEANKATVSILIKYLADGS 420

Query: 421 QDAQTIAAREIRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYD 480
           Q AQT+AAREIRLLAKTGKENRA+IAEAGAIPHL +LL S N +AQENSVTAMLNLSIY+
Sbjct: 421 QAAQTVAAREIRLLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYE 480

Query: 481 KNKSLIMSEVGCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALA 540
           KNKS IM E  CL SI  VL  G T EA+ENAAATLFSLSAVH++KKRIA     VEALA
Sbjct: 481 KNKSRIMEEGDCLESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQCVEALA 540

Query: 541 GLLRNGTPRGKKDAVTALFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALI 600
            LL+NGTPRGKKDAVTAL+NLSTH DNC RMIE G V++LVGAL NEGVAEEAAGALAL+
Sbjct: 541 LLLQNGTPRGKKDAVTALYNLSTHPDNCSRMIEGGGVSSLVGALKNEGVAEEAAGALALL 600

Query: 601 VRQPVGAAAVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPAL 660
           VRQ +GA A+GKE+ AVAGL+ MMRCGTPRGKENAVAALLELCRSGGAA  E+V +APA+
Sbjct: 601 VRQSLGAEAIGKEDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAEKVLRAPAI 660

Query: 661 AGLLQTLLFTGTKRARRKAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVS 713
           AGLLQTLLFTGTKRARRKAASLARVFQR E+ AM  G    G+   TN   D  FT++VS
Sbjct: 661 AGLLQTLLFTGTKRARRKAASLARVFQRRENAAMRSG--VYGFVGNTNGNRDGGFTTDVS 720

BLAST of MC03g0733 vs. ExPASy Swiss-Prot
Match: Q6EUK7 (U-box domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB4 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.8e-149
Identity = 341/739 (46.14%), Postives = 466/739 (63.06%), Query Frame = 0

Query: 1   MAAAAIFSS----------LRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDK 60
           MAAAA  SS           RRRR P   AF AP  L+   L++ +A +A  LV+     
Sbjct: 1   MAAAASASSPVEFLLRRPAPRRRRLPLAGAFFAPTGLAGATLLRAVASLAASLVAGARPP 60

Query: 61  AFFFQRRNSRSLIRRLQVCLVFLE-FLKDSDATLPYT--ALLCLKELYLLLYRSKILLDY 120
           +   QRRN  +L RRL +    LE  L D+ A   ++  A LC +ELY++L+R+++L+ Y
Sbjct: 61  S---QRRNVDALARRLALLSAILESILLDTAAAGAFSDAANLCFRELYVVLFRAELLVSY 120

Query: 121 CSESSKLWLLLQNHSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLK-RA 180
            + + + W LL++  ++  F DL+ EL   LDV P   + L  D    ++LL+   + RA
Sbjct: 121 VASAGRAWALLRSPHLAASFRDLDAELAVVLDVLPAASLRLSHDATGLLDLLRAHCRCRA 180

Query: 181 RIFIDERDE-ALRIRFLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQ 240
                + DE ALR R +  L +F+ G+ P+   ++    D +GI  A SCRAEI++LEEQ
Sbjct: 181 PAQYHDPDEAALRERLMDALRQFDLGQPPDHPSLQSLLAD-MGISTAASCRAEIDYLEEQ 240

Query: 241 IVNHEGDIEPTVAVLYGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKN----LITQEI 300
           I++ E D +  + ++   +A  RY  F +F    D   + A     L  N    L     
Sbjct: 241 ILSQEEDTD--LPLVGSVLALLRYCLFAVF----DPSNAKALRDWPLSGNRQRLLSIGGG 300

Query: 301 AETFLTIPRDFCCPISLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRL 360
            +T  ++P++F CPISLDLM+DPV+ STGQTYDR SI++W+EEGH TCP +GQ L   RL
Sbjct: 301 DDTSFSVPKEFSCPISLDLMRDPVVASTGQTYDRPSIIQWIEEGHSTCPNSGQTLADHRL 360

Query: 361 APNRALRNLIMQWCIAHGVPYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAI 420
            PNRALR+LI QWC  +G+ YD PE  +  AE  A +  +RAA+EAN+ATA IL++ L  
Sbjct: 361 VPNRALRSLISQWCGVYGLQYDSPESNEGMAECVAASCSSRAAMEANKATARILVRMLED 420

Query: 421 GSQDAQTIAAREIRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSI 480
           GS++ + +AA+EIRLLAKTGK+NRAFIA+ GAIP L +LL S + +AQEN+VTA+LNLSI
Sbjct: 421 GSENVKAVAAKEIRLLAKTGKQNRAFIADLGAIPLLCRLLLSNDWMAQENAVTALLNLSI 480

Query: 481 YDKNKSLIMSEVGCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEA 540
           ++ NK  IM + GCL  I  VL+ G TTEA+ENAAATLFSLS VH+FKK I  E GAVE 
Sbjct: 481 FEPNKGRIMEQEGCLRLIVGVLQNGWTTEAKENAAATLFSLSVVHNFKKLIMNEPGAVEE 540

Query: 541 LAGLLRNGTPRGKKDAVTALFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALA 600
           LA +L  GT RGKKDAV ALFNLSTH ++  RM+E+ AV AL+ +L N+ V+EEAAGALA
Sbjct: 541 LASMLTKGTSRGKKDAVMALFNLSTHPESSARMLESCAVVALIQSLRNDTVSEEAAGALA 600

Query: 601 LIVRQPVGAAAVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAP 660
           L+++QP     VG  E  +  L+ +MR GTP+GKENAV+AL E+CR GG+A  ++V K P
Sbjct: 601 LLMKQPSIVHLVGSSETVITSLVGLMRRGTPKGKENAVSALYEICRRGGSALVQRVAKIP 660

Query: 661 ALAGLLQTLLFTGTKRARRKAASLARVFQRCE-HVAMHYGGL-------GVGYAYATNSA 713
            L  ++QT+   GTKRA++KA+ + ++ QR +   AM  G          VG      +A
Sbjct: 661 GLNTVIQTITLNGTKRAKKKASLIVKMCQRSQMPSAMALGSTLTVVDRSLVGNNTLRRAA 720

BLAST of MC03g0733 vs. ExPASy Swiss-Prot
Match: E4NKF8 (U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.2e-104
Identity = 259/686 (37.76%), Postives = 385/686 (56.12%), Query Frame = 0

Query: 16  PSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSRSLIRRLQVCLVFLEF 75
           P  +  ++P  L   +L+ +L L++ E+ S    K    Q +N  S+IRR+++     E 
Sbjct: 4   PRSKMMISPGLLPTESLLDSLILISNEVSS--MQKFPLVQIKNVSSMIRRIKLLSSLFEE 63

Query: 76  LKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQNHSISGHFNDLNLELL 135
           +++SD+ LP +++LC  E++ ++ R K+L+  C++ S LW L+Q   IS  F  L  E+ 
Sbjct: 64  IQESDSPLPPSSILCFIEIFSVITRVKVLIQECTDGSSLWSLIQLDFISNQFFVLVKEMG 123

Query: 136 TFLDVFPLEEVELGVDIREQVELLQKQLKRA--RIFIDERDEALRIRFLLFLEE--FENG 195
             LD+ PL  + +  DI+EQV+LL KQ KR    +FID R+   R      + +   +N 
Sbjct: 124 RALDILPLNLLNVAQDIKEQVDLLHKQSKRVELELFIDPREVQRRENLFEVMSKNCLQNK 183

Query: 196 RLPNPREMREF-----FVDKLGIRNAKSCRAEIEFLEEQIVNHEGD----IEPTVAVLYG 255
           +  N +   +F      +  +G+R       EI  LE +  N  G     +   +  L  
Sbjct: 184 KTNNNKGFIDFVKVEEIMCSIGLRTLSDYVEEISKLEVEAQNQAGTGGLIVVSNINNLMS 243

Query: 256 FVAFTRYSRFFLFGFEED--EIESGATNQKKLKKN--LITQEIAETFLT--IPRDFCCPI 315
            V++T+   F   G  E+   I     N+ K+  N    +   +++ +T  IP +F CPI
Sbjct: 244 LVSYTKSMVFRNDGESEECKPISMFLYNKSKIHDNDSSSSSSFSQSMMTVNIPDEFRCPI 303

Query: 316 SLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCI 375
           SLDLM+DPVIVS+G TYDR SI  W+  GH TCPK+GQ LIHT L PN AL++L+ QWC 
Sbjct: 304 SLDLMRDPVIVSSGHTYDRISIAEWINSGHHTCPKSGQRLIHTALIPNYALKSLVHQWCY 363

Query: 376 AHGVPYDPPEGMDPSAES--------FATASPTRAALEANRATAAILIQQLAIGSQDAQT 435
            + V  +     + ++ S            S  +A+ +A + TA  L+ +LA GS D Q 
Sbjct: 364 ENNVKMNEAITKNNNSSSKRHKNENAIDHISENKASKDAVKMTAEFLVGKLATGSTDIQR 423

Query: 436 IAAREIRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSL 495
            +A EIRLLAKTG +NR  IAE GAIP L  LL S +   QE+ VTA+ NLSIYD NK L
Sbjct: 424 QSAYEIRLLAKTGMDNRRIIAEVGAIPFLVTLLVSKDSRIQEHVVTALFNLSIYDNNKIL 483

Query: 496 IMSEVGCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRN 555
           IM+  G + +I EVL FG T EARENAAA ++SLS + D K +I     A+ AL GLL+ 
Sbjct: 484 IMA-AGAIDNIVEVLEFGKTMEARENAAAAIYSLSMIDDCKVQIGASSRAIPALVGLLKE 543

Query: 556 GTPRGKKDAVTALFNLSTHTDNCVRMIEAGAVTALVGALGNE--GVAEEAAGALALIVRQ 615
           GT  GK+DA TALFNL+ +  N + ++++GAVT LV  L ++  G+ +++   LA+++  
Sbjct: 544 GTIIGKRDAATALFNLAVYNPNKLSIVKSGAVTLLVELLMDDKAGITDDSLAVLAVLLGC 603

Query: 616 PVGAAAVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGL 673
             G   +   +  V  LI ++R G+ +GKEN++  LL LC+  G     ++   P     
Sbjct: 604 SEGLEEIKNSKSLVPLLIDLLRFGSVKGKENSITLLLGLCKEEGELVAMRLLANPRSIPS 663

BLAST of MC03g0733 vs. ExPASy Swiss-Prot
Match: Q9LZW3 (U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 7.6e-95
Identity = 243/669 (36.32%), Postives = 372/669 (55.61%), Query Frame = 0

Query: 8   SSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSRSLIRRLQ 67
           S  R+RR   + +F +P   SD  L ++L L + E+ S       F  RRNS SLIR+++
Sbjct: 9   SPARKRRPLVVGSFESPKLSSDTKLTRSLFLASHEISS--MQPLPFILRRNSLSLIRKVK 68

Query: 68  V-CLVFLEFLKDSDATLPY--TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQNHSIS 127
           +   VF E L      + Y  +A LC +E+ +++ R K L+D CS  SKLWLLLQ   ++
Sbjct: 69  ILASVFDELLLPRSQLVVYSQSAHLCFEEMQIVMQRIKSLIDDCSRVSKLWLLLQIDIVA 128

Query: 128 GHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIRFLLF 187
            +F++L  +L T LD+ PL + +L  D ++ + LL KQ   +  F+D RD ALR +    
Sbjct: 129 FNFHELVTDLSTVLDILPLHDFDLSDDAQDLISLLTKQCSDSVQFVDARDVALRRKVTDT 188

Query: 188 LEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVLYGFV 247
           +   ++   P+   + + F D LG+ ++ S   EI+ LE++I +   D   + A     +
Sbjct: 189 IAGIKHQISPDHSTLIKIFND-LGLSDSASLTDEIQRLEDEIQDQIDDRSKSAAA--SLI 248

Query: 248 AFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDLMKDP 307
              RYS+  L+G         +T     +++   Q +++    IP DF CPI+L+LM+DP
Sbjct: 249 GLVRYSKCVLYG--------PSTPAPDFRRH---QSLSDA--NIPADFRCPITLELMRDP 308

Query: 308 VIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGVPYDP 367
           V+V+TGQTYDR SI  W++ GH TCPKTGQ+L HT L PNRAL+NLI+ WC    +P+  
Sbjct: 309 VVVATGQTYDRESIDLWIQSGHNTCPKTGQVLKHTSLVPNRALKNLIVLWCRDQKIPF-- 368

Query: 368 PEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKTGKEN 427
               +   +     +P + A+E  +   + LI++L++   +       E+R LAK+    
Sbjct: 369 ----ELYGDGGGEPAPCKEAVEFTKMMVSFLIEKLSVADSNGVVF---ELRALAKSDTVA 428

Query: 428 RAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSITEVLR 487
           RA IAEAGAIP L + LA+  P  Q N+VT +LNLSI ++NK+ IM   G L  + EVLR
Sbjct: 429 RACIAEAGAIPKLVRYLATECPSLQINAVTTILNLSILEQNKTRIMETDGALNGVIEVLR 488

Query: 488 FGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTALFNL 547
            G T EA+ NAAATLFSL+ V  +++R+  +   V  L  L + G    K+DA+ A+ NL
Sbjct: 489 SGATWEAKANAAATLFSLAGVSAYRRRLGRKARVVSGLVDLAKQGPTSSKRDALVAILNL 548

Query: 548 STHTDNCVRMIEAGAVTALVGALGN--EGVAEEAAGALALIVRQPVGAAAVGKEERAVAG 607
               +N  R +EAG    ++GA G+  + + EEA   +  +VR+  G  AV      +  
Sbjct: 549 VAERENVGRFVEAG----VMGAAGDAFQELPEEAVAVVEAVVRRG-GLMAVSAAFSLIRL 608

Query: 608 LIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARRKA 667
           L  +MR G    +E+A A L+ +CR GG+    ++   P +  ++  ++  GT R  RKA
Sbjct: 609 LGEVMREGADTTRESAAATLVTMCRKGGSELVAEMAAIPGIERVIWEMIGAGTARGGRKA 645

Query: 668 ASLARVFQR 672
           ASL R  +R
Sbjct: 669 ASLMRYLRR 645

BLAST of MC03g0733 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.3e-83
Identity = 233/618 (37.70%), Postives = 329/618 (53.24%), Query Frame = 0

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           +L RRL++ +   E +++S+  +    L  L  L   +  +K  L +CS+ SK++L+++ 
Sbjct: 35  NLARRLKLLVPMFEEIRESNEPISEDTLKTLMNLKEAMCSAKDYLKFCSQGSKIYLVMER 94

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
             ++    +++++L   L   P EE+++  ++REQVEL+  Q +RA+  +D  D+ L   
Sbjct: 95  EQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQVELVLSQFRRAKGRVDVSDDELYED 154

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGD----IEPT 240
                 +  +     P  + E    KL +        E   L E + +  GD    IE  
Sbjct: 155 LQSLCNKSSDVDAYQP--VLERVAKKLHLMEIPDLAQESVALHEMVASSGGDVGENIEEM 214

Query: 241 VAVLYGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPI 300
             VL     F +         E+D  E              +   ++    IP DF CPI
Sbjct: 215 AMVLKMIKDFVQ--------TEDDNGEEQKVGVNSRSNGQTSTAASQKIPVIPDDFRCPI 274

Query: 301 SLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCI 360
           SL++M+DPVIVS+GQTY+R+ I +W+E GH TCPKT Q L  T L PN  LR+LI QWC 
Sbjct: 275 SLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRSLIAQWCE 334

Query: 361 AHGV-PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIR 420
           A+ + P  PP  + P   S + +SP     EAN+     L+ +LA G+ + Q  AA EIR
Sbjct: 335 ANDIEPPKPPSSLRPRKVS-SFSSPA----EANKIED--LMWRLAYGNPEDQRSAAGEIR 394

Query: 421 LLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGC 480
           LLAK   +NR  IAEAGAIP L  LL++P+   QE+SVTA+LNLSI + NK  I+S  G 
Sbjct: 395 LLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICENNKGAIVS-AGA 454

Query: 481 LGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKK 540
           +  I +VL+ G + EARENAAATLFSLS + + K  I    GA+  L  LL  GT RGKK
Sbjct: 455 IPGIVQVLKKG-SMEARENAAATLFSLSVIDENKVTIGAL-GAIPPLVVLLNEGTQRGKK 514

Query: 541 DAVTALFNLSTHTDNCVRMIEAGAVTALVGAL--GNEGVAEEAAGALALIVRQPVGAAAV 600
           DA TALFNL  +  N  + I AG +  L   L     G+ +EA   LA++   P G A +
Sbjct: 515 DAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSSHPEGKAII 574

Query: 601 GKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFT 660
           G  + AV  L+  +R G+PR +ENA A L+ LC SG      +  K   L G L  L   
Sbjct: 575 GSSD-AVPSLVEFIRTGSPRNRENAAAVLVHLC-SGDPQHLVEAQKL-GLMGPLIDLAGN 629

Query: 661 GTKRARRKAASLARVFQR 672
           GT R +RKAA L     R
Sbjct: 635 GTDRGKRKAAQLLERISR 629

BLAST of MC03g0733 vs. NCBI nr
Match: XP_022140088.1 (U-box domain-containing protein 17 [Momordica charantia])

HSP 1 Score: 1350 bits (3494), Expect = 0.0
Identity = 713/713 (100.00%), Postives = 713/713 (100.00%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR
Sbjct: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
           YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV
Sbjct: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT
Sbjct: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA
Sbjct: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV
Sbjct: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV 713

BLAST of MC03g0733 vs. NCBI nr
Match: XP_008443533.1 (PREDICTED: U-box domain-containing protein 17 [Cucumis melo] >KAA0057064.1 U-box domain-containing protein 17 [Cucumis melo var. makuwa])

HSP 1 Score: 1249 bits (3233), Expect = 0.0
Identity = 657/715 (91.89%), Postives = 688/715 (96.22%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLA+VATELVSRFS+K+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLAIVATELVSRFSEKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFLEFLK++DA LP+TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRKLEVCLVFLEFLKETDANLPHTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP+EEVELG D+REQVELLQKQL+R R+F+DERDE LR  
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPIEEVELGADVREQVELLQKQLRRTRLFVDERDEVLRTC 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPREMREFFVDKL I NAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLSFLDEFENGRLPNPREMREFFVDKLKIWNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVAFTRYSRFFLFGFEED+++SG +NQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 NGFVAFTRYSRFFLFGFEEDDVDSGTSNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPV +STGQTYDRSSI RWMEEGH TCPKTGQMLIHTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MKDPVTISTGQTYDRSSITRWMEEGHNTCPKTGQMLIHTRLAPNRALRNLIVQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPP+GMD SAES+A ASPTRAALEANRATA ILIQQL+IGSQDA+TIAAREIRLLAKT
Sbjct: 361 PYDPPDGMDASAESYAIASPTRAALEANRATALILIQQLSIGSQDAKTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPN VAQENSVTAMLNLSIYDKNKSLIMSEVGCL +IT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNAVAQENSVTAMLNLSIYDKNKSLIMSEVGCLRAIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLRFGH+TEARENAAATLFSLSAVHD+KKRIAEEGGAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 EVLRFGHSTEARENAAATLFSLSAVHDYKKRIAEEGGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCV+MIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV KEERAV
Sbjct: 541 LFNLSTHTDNCVQMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVAKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MHYGGLGVGYAYA+NSAS  + SF SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVTMHYGGLGVGYAYASNSASSRETSFASEVSVPISISVPVV 715

BLAST of MC03g0733 vs. NCBI nr
Match: XP_004147388.1 (U-box domain-containing protein 17 [Cucumis sativus] >KGN65604.1 hypothetical protein Csa_019666 [Cucumis sativus])

HSP 1 Score: 1249 bits (3231), Expect = 0.0
Identity = 654/715 (91.47%), Postives = 688/715 (96.22%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTL +VATELVSRFSDK+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLTIVATELVSRFSDKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFLEFLK++DA LP+TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRKLEVCLVFLEFLKETDANLPHTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP+EEVELG D+REQVELLQKQL+R R+F+DERDE LR R
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPIEEVELGADVREQVELLQKQLRRTRLFVDERDEVLRTR 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPREMREFFVDKL I NAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLSFLDEFENGRLPNPREMREFFVDKLKIWNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVA TRYSRFFLFGFEED+++SG +NQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 NGFVALTRYSRFFLFGFEEDDVDSGTSNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPV +STGQTYDRSSI RWMEEGH TCPKTGQMLIHTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MKDPVTISTGQTYDRSSITRWMEEGHNTCPKTGQMLIHTRLAPNRALRNLIVQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPP+GMD S+ES+A ASPTRAALEANRATA ILIQQL+IGSQDA+TIAAREIRLLAKT
Sbjct: 361 PYDPPDGMDASSESYAIASPTRAALEANRATAMILIQQLSIGSQDAKTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPN VAQENSVTAMLNLSIYDKNKSLIMSEVGCLG+IT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNAVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGAIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           +VLRFGH+TEARENAAATLFSLSAVHD+KKRIAEEGGAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 DVLRFGHSTEARENAAATLFSLSAVHDYKKRIAEEGGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCV+MIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV K+ERAV
Sbjct: 541 LFNLSTHTDNCVQMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVAKQERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MHYGGLGVGYAYA+NSAS  + +F SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVTMHYGGLGVGYAYASNSASNRETNFASEVSVPISISVPVV 715

BLAST of MC03g0733 vs. NCBI nr
Match: XP_038894534.1 (U-box domain-containing protein 17 [Benincasa hispida])

HSP 1 Score: 1246 bits (3223), Expect = 0.0
Identity = 654/715 (91.47%), Postives = 687/715 (96.08%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLA+VAT LVSRFS+K+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLAIVATGLVSRFSEKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFLEFLK++DA LP+TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRKLEVCLVFLEFLKETDANLPHTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFPLEEVELGVD+REQ+ELLQKQL+R R+F+DER+E LR R
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPLEEVELGVDVREQLELLQKQLRRTRMFVDEREEVLRTR 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPREMREFFVDKL IRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLSFLDEFENGRLPNPREMREFFVDKLKIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVAFTRYSRFFLFGFEEDE++SGA+NQKKLKKNLI+QEIAETFLT+PRDFCCPISLDL
Sbjct: 241 NGFVAFTRYSRFFLFGFEEDEVDSGASNQKKLKKNLISQEIAETFLTVPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPV +STGQTYDRSSI RWMEEGH  CPKTGQ+LIHTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MKDPVTISTGQTYDRSSITRWMEEGHNNCPKTGQVLIHTRLAPNRALRNLIVQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPPEGMD SAES+A ASPTRAALEANRATA ILIQQL+IGSQDA+TIAAREIRLLAKT
Sbjct: 361 PYDPPEGMDASAESYAMASPTRAALEANRATAMILIQQLSIGSQDAKTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQ LLAS NPVAQENSVTAMLNLSIYDKNKSLIMSE+GCLG+IT
Sbjct: 421 GKENRAFIAEAGAIPHLQTLLASQNPVAQENSVTAMLNLSIYDKNKSLIMSEIGCLGAIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           +VLRFGH+TEARENAAATLFSLSAVHD+KKRIAEE GAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 KVLRFGHSTEARENAAATLFSLSAVHDYKKRIAEEDGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV KEERAV
Sbjct: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVTKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
            GLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 GGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MHYGGLGVGYAYA+NSA   D SF SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVTMHYGGLGVGYAYASNSAPSRDSSFASEVSVPISISVPVV 715

BLAST of MC03g0733 vs. NCBI nr
Match: KAG6583941.1 (U-box domain-containing protein 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1216 bits (3145), Expect = 0.0
Identity = 643/715 (89.93%), Postives = 675/715 (94.41%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLE FLAPVDLSDVALV+TLA+VATELVSRFS K+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEVFLAPVDLSDVALVRTLAVVATELVSRFSQKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFL+ LKDS ATLP+TALLCLKELYLLLYRSKILLDYCSESSKLW+LLQN
Sbjct: 61  SLIRKLEVCLVFLDCLKDSGATLPHTALLCLKELYLLLYRSKILLDYCSESSKLWMLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP EEVEL VD+REQVELLQKQL+R R+F+DE DE LR  
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPFEEVELAVDVREQVELLQKQLRRTRMFVDECDEVLRAH 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPRE+REFFVDKL IRNAK CRAEIEFLEEQIVNHEGD+EPT+AVL
Sbjct: 181 FLSFLDEFENGRLPNPRELREFFVDKLKIRNAKCCRAEIEFLEEQIVNHEGDVEPTLAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVAFTRYSRF LFGFEEDE++SGA NQKKLKKNLI+QEIAET LTIPRDFCCPISLDL
Sbjct: 241 NGFVAFTRYSRFLLFGFEEDEVDSGAGNQKKLKKNLISQEIAETLLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           M DPVI+STGQTYDRSSI RWMEEGH TCPKTGQ+L+HTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MNDPVIISTGQTYDRSSITRWMEEGHSTCPKTGQLLVHTRLAPNRALRNLIIQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPPEGMD SAES+A ASPTRAALEANRATA ILIQQL+IGSQDA++IAAREIRLLAKT
Sbjct: 361 PYDPPEGMDASAESYAMASPTRAALEANRATAIILIQQLSIGSQDAKSIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLR GH+TEARENAAATLFSLSAVHD+KKRIAEE GAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 EVLRSGHSTEARENAAATLFSLSAVHDYKKRIAEESGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCV+MIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV KEERAV
Sbjct: 541 LFNLSTHTDNCVQMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVAKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQ LLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQMLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MH    GVGYAY +NSAS  D SF SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVVMH----GVGYAYPSNSASNRDSSFASEVSVPISISVPVV 711

BLAST of MC03g0733 vs. ExPASy TrEMBL
Match: A0A6J1CH75 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111010827 PE=4 SV=1)

HSP 1 Score: 1350 bits (3494), Expect = 0.0
Identity = 713/713 (100.00%), Postives = 713/713 (100.00%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR
Sbjct: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
           YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV
Sbjct: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT
Sbjct: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA
Sbjct: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV
Sbjct: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVSVPISISVPVV 713

BLAST of MC03g0733 vs. ExPASy TrEMBL
Match: A0A5A7UPD8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002530 PE=4 SV=1)

HSP 1 Score: 1249 bits (3233), Expect = 0.0
Identity = 657/715 (91.89%), Postives = 688/715 (96.22%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLA+VATELVSRFS+K+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLAIVATELVSRFSEKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFLEFLK++DA LP+TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRKLEVCLVFLEFLKETDANLPHTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP+EEVELG D+REQVELLQKQL+R R+F+DERDE LR  
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPIEEVELGADVREQVELLQKQLRRTRLFVDERDEVLRTC 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPREMREFFVDKL I NAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLSFLDEFENGRLPNPREMREFFVDKLKIWNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVAFTRYSRFFLFGFEED+++SG +NQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 NGFVAFTRYSRFFLFGFEEDDVDSGTSNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPV +STGQTYDRSSI RWMEEGH TCPKTGQMLIHTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MKDPVTISTGQTYDRSSITRWMEEGHNTCPKTGQMLIHTRLAPNRALRNLIVQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPP+GMD SAES+A ASPTRAALEANRATA ILIQQL+IGSQDA+TIAAREIRLLAKT
Sbjct: 361 PYDPPDGMDASAESYAIASPTRAALEANRATALILIQQLSIGSQDAKTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPN VAQENSVTAMLNLSIYDKNKSLIMSEVGCL +IT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNAVAQENSVTAMLNLSIYDKNKSLIMSEVGCLRAIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLRFGH+TEARENAAATLFSLSAVHD+KKRIAEEGGAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 EVLRFGHSTEARENAAATLFSLSAVHDYKKRIAEEGGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCV+MIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV KEERAV
Sbjct: 541 LFNLSTHTDNCVQMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVAKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MHYGGLGVGYAYA+NSAS  + SF SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVTMHYGGLGVGYAYASNSASSRETSFASEVSVPISISVPVV 715

BLAST of MC03g0733 vs. ExPASy TrEMBL
Match: A0A1S3B912 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487100 PE=4 SV=1)

HSP 1 Score: 1249 bits (3233), Expect = 0.0
Identity = 657/715 (91.89%), Postives = 688/715 (96.22%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLA+VATELVSRFS+K+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLAIVATELVSRFSEKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFLEFLK++DA LP+TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRKLEVCLVFLEFLKETDANLPHTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP+EEVELG D+REQVELLQKQL+R R+F+DERDE LR  
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPIEEVELGADVREQVELLQKQLRRTRLFVDERDEVLRTC 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPREMREFFVDKL I NAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLSFLDEFENGRLPNPREMREFFVDKLKIWNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVAFTRYSRFFLFGFEED+++SG +NQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 NGFVAFTRYSRFFLFGFEEDDVDSGTSNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPV +STGQTYDRSSI RWMEEGH TCPKTGQMLIHTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MKDPVTISTGQTYDRSSITRWMEEGHNTCPKTGQMLIHTRLAPNRALRNLIVQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPP+GMD SAES+A ASPTRAALEANRATA ILIQQL+IGSQDA+TIAAREIRLLAKT
Sbjct: 361 PYDPPDGMDASAESYAIASPTRAALEANRATALILIQQLSIGSQDAKTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPN VAQENSVTAMLNLSIYDKNKSLIMSEVGCL +IT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNAVAQENSVTAMLNLSIYDKNKSLIMSEVGCLRAIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLRFGH+TEARENAAATLFSLSAVHD+KKRIAEEGGAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 EVLRFGHSTEARENAAATLFSLSAVHDYKKRIAEEGGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCV+MIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV KEERAV
Sbjct: 541 LFNLSTHTDNCVQMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVAKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MHYGGLGVGYAYA+NSAS  + SF SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVTMHYGGLGVGYAYASNSASSRETSFASEVSVPISISVPVV 715

BLAST of MC03g0733 vs. ExPASy TrEMBL
Match: A0A0A0LX59 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G467720 PE=4 SV=1)

HSP 1 Score: 1249 bits (3231), Expect = 0.0
Identity = 654/715 (91.47%), Postives = 688/715 (96.22%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTL +VATELVSRFSDK+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLTIVATELVSRFSDKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFLEFLK++DA LP+TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN
Sbjct: 61  SLIRKLEVCLVFLEFLKETDANLPHTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP+EEVELG D+REQVELLQKQL+R R+F+DERDE LR R
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPIEEVELGADVREQVELLQKQLRRTRLFVDERDEVLRTR 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPREMREFFVDKL I NAKSCRAEIEFLEEQIVNHEGDIEPTVAVL
Sbjct: 181 FLSFLDEFENGRLPNPREMREFFVDKLKIWNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVA TRYSRFFLFGFEED+++SG +NQKKLKKNLITQEIAETFLTIPRDFCCPISLDL
Sbjct: 241 NGFVALTRYSRFFLFGFEEDDVDSGTSNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           MKDPV +STGQTYDRSSI RWMEEGH TCPKTGQMLIHTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MKDPVTISTGQTYDRSSITRWMEEGHNTCPKTGQMLIHTRLAPNRALRNLIVQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPP+GMD S+ES+A ASPTRAALEANRATA ILIQQL+IGSQDA+TIAAREIRLLAKT
Sbjct: 361 PYDPPDGMDASSESYAIASPTRAALEANRATAMILIQQLSIGSQDAKTIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPN VAQENSVTAMLNLSIYDKNKSLIMSEVGCLG+IT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNAVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGAIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           +VLRFGH+TEARENAAATLFSLSAVHD+KKRIAEEGGAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 DVLRFGHSTEARENAAATLFSLSAVHDYKKRIAEEGGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCV+MIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGA AV K+ERAV
Sbjct: 541 LFNLSTHTDNCVQMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAEAVAKQERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MHYGGLGVGYAYA+NSAS  + +F SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVTMHYGGLGVGYAYASNSASNRETNFASEVSVPISISVPVV 715

BLAST of MC03g0733 vs. ExPASy TrEMBL
Match: A0A6J1EI41 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434297 PE=4 SV=1)

HSP 1 Score: 1214 bits (3140), Expect = 0.0
Identity = 643/715 (89.93%), Postives = 674/715 (94.27%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MAAAAIFSSLRRRRSPSLE FLAPVDLSDVALV+TLA+VATELVSRFS K+FFFQRRNSR
Sbjct: 1   MAAAAIFSSLRRRRSPSLEVFLAPVDLSDVALVRTLAVVATELVSRFSQKSFFFQRRNSR 60

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           SLIR+L+VCLVFL+ LKDS ATLP+TALLCLKELYLLLYRSKILLDYCSESSKLW+LLQN
Sbjct: 61  SLIRKLEVCLVFLDCLKDSGATLPHTALLCLKELYLLLYRSKILLDYCSESSKLWMLLQN 120

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
           HSISGHFNDLNLELLTF DVFP EEVEL VD+REQVELLQKQL+R R+F+DE DE LR  
Sbjct: 121 HSISGHFNDLNLELLTFFDVFPFEEVELAVDVREQVELLQKQLRRTRMFVDECDEVLRAH 180

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVL 240
           FL FL+EFENGRLPNPRE+REFFVDKL IRNAK CRAEIEFLEEQIVNHEGD+EPT+AVL
Sbjct: 181 FLSFLDEFENGRLPNPRELREFFVDKLKIRNAKCCRAEIEFLEEQIVNHEGDVEPTLAVL 240

Query: 241 YGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDL 300
            GFVAFTRYSRF LFGFEEDE++SGA NQKKLKKNLI+QEIAET LTIPRDFCCPISLDL
Sbjct: 241 NGFVAFTRYSRFLLFGFEEDEVDSGAGNQKKLKKNLISQEIAETLLTIPRDFCCPISLDL 300

Query: 301 MKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGV 360
           M DPVI+STGQTYDRSSI RWMEEGH TCPKTGQ+L+HTRLAPNRALRNLI+QWCIAHGV
Sbjct: 301 MNDPVIISTGQTYDRSSITRWMEEGHSTCPKTGQLLVHTRLAPNRALRNLIIQWCIAHGV 360

Query: 361 PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKT 420
           PYDPPEGMD SAES+A ASPTRAALEANRATA ILIQQL+IGSQDA++IAAREIRLLAKT
Sbjct: 361 PYDPPEGMDASAESYAMASPTRAALEANRATAIILIQQLSIGSQDAKSIAAREIRLLAKT 420

Query: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480
           GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT
Sbjct: 421 GKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSIT 480

Query: 481 EVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTA 540
           EVLR GH+TEARENAAATLFSLSAVHD+KKRIAEE GAVEALAGLLR+GTPRGKKDAVTA
Sbjct: 481 EVLRSGHSTEARENAAATLFSLSAVHDYKKRIAEESGAVEALAGLLRDGTPRGKKDAVTA 540

Query: 541 LFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALIVRQPVGAAAVGKEERAV 600
           LFNLSTHTDNCVRMIEAGAVTALV ALGNEGVAEEAAGALALIVRQPVGA AV KEERAV
Sbjct: 541 LFNLSTHTDNCVRMIEAGAVTALVVALGNEGVAEEAAGALALIVRQPVGAEAVAKEERAV 600

Query: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARR 660
           AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQ LLFTGTKRARR
Sbjct: 601 AGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQMLLFTGTKRARR 660

Query: 661 KAASLARVFQRCEHVAMHYGGLGVGYAYATNSAS--DPSFTSEVSVPISISVPVV 713
           KAASLARVFQRCEHV MH    GVGYAY +NSAS  D SF SEVSVPISISVPVV
Sbjct: 661 KAASLARVFQRCEHVVMH----GVGYAYPSNSASNRDSSFASEVSVPISISVPVV 711

BLAST of MC03g0733 vs. TAIR 10
Match: AT1G29340.1 (plant U-box 17 )

HSP 1 Score: 918.3 bits (2372), Expect = 3.8e-267
Identity = 498/729 (68.31%), Postives = 592/729 (81.21%), Query Frame = 0

Query: 1   MAAAAIFSSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSR 60
           MA+AAIFSSLRRRRSPSLEAFLAPVDLS VALVQTLA +++E+VS F+   F FQR+N+R
Sbjct: 1   MASAAIFSSLRRRRSPSLEAFLAPVDLSGVALVQTLASISSEVVSCFTSVRFSFQRKNAR 60

Query: 61  SLIRRLQVCLVFLEFLKDSD----------------ATLPYTALLCLKELYLLLYRSKIL 120
           SLIR++++ +V  EFL DS+                +    TALLCLKELYLLLYRSKIL
Sbjct: 61  SLIRKIEIFVVLFEFLVDSNWGSTTTRTRARRRSKSSVSESTALLCLKELYLLLYRSKIL 120

Query: 121 LDYCSESSKLWLLLQNHSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLK 180
           +DYC++SSKLWLLLQN SISG+F+DLN E+ T LDV P+ ++ L  DIREQ+ELLQ+Q +
Sbjct: 121 VDYCAQSSKLWLLLQNPSISGYFHDLNQEISTLLDVLPVNDLGLSDDIREQIELLQRQSR 180

Query: 181 RARIFIDERDEALRIRFLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEE 240
           +AR++ID+ DE+LR  F  FL+ FENG++P+  ++R FFV+KLGIR++KSCR+EIEFLEE
Sbjct: 181 KARLYIDKNDESLRESFYSFLDGFENGKIPSSVDLRMFFVEKLGIRDSKSCRSEIEFLEE 240

Query: 241 QIVNHEGDIEPTVAVLYGFVAFTRYSRFFLFGFEEDEIESG-ATNQKKLKKNLITQEIAE 300
           QIVNH+GD+EPT +V+ GFVA TRY RF LFGFEED +E     N KK +K  + QEI +
Sbjct: 241 QIVNHDGDLEPTGSVINGFVAITRYCRFLLFGFEEDGMEWWIENNPKKPRKGFVAQEIGD 300

Query: 301 TFLTIPRDFCCPISLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAP 360
           TF+T+P+DF CPISLDLM DPVI+STGQTYDR+SI RW+EEGHCTCPKTGQML+ +R+ P
Sbjct: 301 TFITVPKDFVCPISLDLMTDPVIISTGQTYDRNSIARWIEEGHCTCPKTGQMLMDSRIVP 360

Query: 361 NRALRNLIMQWCIAHGVPYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGS 420
           NRAL+NLI+QWC A G+ Y+  E  D   ESFA+A PT+AA+EAN+AT +ILI+ LA GS
Sbjct: 361 NRALKNLIVQWCTASGISYE-SEFTDSPNESFASALPTKAAVEANKATVSILIKYLADGS 420

Query: 421 QDAQTIAAREIRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYD 480
           Q AQT+AAREIRLLAKTGKENRA+IAEAGAIPHL +LL S N +AQENSVTAMLNLSIY+
Sbjct: 421 QAAQTVAAREIRLLAKTGKENRAYIAEAGAIPHLCRLLTSENAIAQENSVTAMLNLSIYE 480

Query: 481 KNKSLIMSEVGCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALA 540
           KNKS IM E  CL SI  VL  G T EA+ENAAATLFSLSAVH++KKRIA     VEALA
Sbjct: 481 KNKSRIMEEGDCLESIVSVLVSGLTVEAQENAAATLFSLSAVHEYKKRIAIVDQCVEALA 540

Query: 541 GLLRNGTPRGKKDAVTALFNLSTHTDNCVRMIEAGAVTALVGALGNEGVAEEAAGALALI 600
            LL+NGTPRGKKDAVTAL+NLSTH DNC RMIE G V++LVGAL NEGVAEEAAGALAL+
Sbjct: 541 LLLQNGTPRGKKDAVTALYNLSTHPDNCSRMIEGGGVSSLVGALKNEGVAEEAAGALALL 600

Query: 601 VRQPVGAAAVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPAL 660
           VRQ +GA A+GKE+ AVAGL+ MMRCGTPRGKENAVAALLELCRSGGAA  E+V +APA+
Sbjct: 601 VRQSLGAEAIGKEDSAVAGLMGMMRCGTPRGKENAVAALLELCRSGGAAVAEKVLRAPAI 660

Query: 661 AGLLQTLLFTGTKRARRKAASLARVFQRCEHVAMHYGGLGVGYAYATNSASDPSFTSEVS 713
           AGLLQTLLFTGTKRARRKAASLARVFQR E+ AM  G    G+   TN   D  FT++VS
Sbjct: 661 AGLLQTLLFTGTKRARRKAASLARVFQRRENAAMRSG--VYGFVGNTNGNRDGGFTTDVS 720

BLAST of MC03g0733 vs. TAIR 10
Match: AT5G01830.1 (ARM repeat superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 5.4e-96
Identity = 243/669 (36.32%), Postives = 372/669 (55.61%), Query Frame = 0

Query: 8   SSLRRRRSPSLEAFLAPVDLSDVALVQTLALVATELVSRFSDKAFFFQRRNSRSLIRRLQ 67
           S  R+RR   + +F +P   SD  L ++L L + E+ S       F  RRNS SLIR+++
Sbjct: 9   SPARKRRPLVVGSFESPKLSSDTKLTRSLFLASHEISS--MQPLPFILRRNSLSLIRKVK 68

Query: 68  V-CLVFLEFLKDSDATLPY--TALLCLKELYLLLYRSKILLDYCSESSKLWLLLQNHSIS 127
           +   VF E L      + Y  +A LC +E+ +++ R K L+D CS  SKLWLLLQ   ++
Sbjct: 69  ILASVFDELLLPRSQLVVYSQSAHLCFEEMQIVMQRIKSLIDDCSRVSKLWLLLQIDIVA 128

Query: 128 GHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIRFLLF 187
            +F++L  +L T LD+ PL + +L  D ++ + LL KQ   +  F+D RD ALR +    
Sbjct: 129 FNFHELVTDLSTVLDILPLHDFDLSDDAQDLISLLTKQCSDSVQFVDARDVALRRKVTDT 188

Query: 188 LEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGDIEPTVAVLYGFV 247
           +   ++   P+   + + F D LG+ ++ S   EI+ LE++I +   D   + A     +
Sbjct: 189 IAGIKHQISPDHSTLIKIFND-LGLSDSASLTDEIQRLEDEIQDQIDDRSKSAAA--SLI 248

Query: 248 AFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPISLDLMKDP 307
              RYS+  L+G         +T     +++   Q +++    IP DF CPI+L+LM+DP
Sbjct: 249 GLVRYSKCVLYG--------PSTPAPDFRRH---QSLSDA--NIPADFRCPITLELMRDP 308

Query: 308 VIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCIAHGVPYDP 367
           V+V+TGQTYDR SI  W++ GH TCPKTGQ+L HT L PNRAL+NLI+ WC    +P+  
Sbjct: 309 VVVATGQTYDRESIDLWIQSGHNTCPKTGQVLKHTSLVPNRALKNLIVLWCRDQKIPF-- 368

Query: 368 PEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIRLLAKTGKEN 427
               +   +     +P + A+E  +   + LI++L++   +       E+R LAK+    
Sbjct: 369 ----ELYGDGGGEPAPCKEAVEFTKMMVSFLIEKLSVADSNGVVF---ELRALAKSDTVA 428

Query: 428 RAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGCLGSITEVLR 487
           RA IAEAGAIP L + LA+  P  Q N+VT +LNLSI ++NK+ IM   G L  + EVLR
Sbjct: 429 RACIAEAGAIPKLVRYLATECPSLQINAVTTILNLSILEQNKTRIMETDGALNGVIEVLR 488

Query: 488 FGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKKDAVTALFNL 547
            G T EA+ NAAATLFSL+ V  +++R+  +   V  L  L + G    K+DA+ A+ NL
Sbjct: 489 SGATWEAKANAAATLFSLAGVSAYRRRLGRKARVVSGLVDLAKQGPTSSKRDALVAILNL 548

Query: 548 STHTDNCVRMIEAGAVTALVGALGN--EGVAEEAAGALALIVRQPVGAAAVGKEERAVAG 607
               +N  R +EAG    ++GA G+  + + EEA   +  +VR+  G  AV      +  
Sbjct: 549 VAERENVGRFVEAG----VMGAAGDAFQELPEEAVAVVEAVVRRG-GLMAVSAAFSLIRL 608

Query: 608 LIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFTGTKRARRKA 667
           L  +MR G    +E+A A L+ +CR GG+    ++   P +  ++  ++  GT R  RKA
Sbjct: 609 LGEVMREGADTTRESAAATLVTMCRKGGSELVAEMAAIPGIERVIWEMIGAGTARGGRKA 645

Query: 668 ASLARVFQR 672
           ASL R  +R
Sbjct: 669 ASLMRYLRR 645

BLAST of MC03g0733 vs. TAIR 10
Match: AT3G46510.1 (plant U-box 13 )

HSP 1 Score: 312.4 bits (799), Expect = 9.5e-85
Identity = 233/618 (37.70%), Postives = 329/618 (53.24%), Query Frame = 0

Query: 61  SLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKLWLLLQN 120
           +L RRL++ +   E +++S+  +    L  L  L   +  +K  L +CS+ SK++L+++ 
Sbjct: 35  NLARRLKLLVPMFEEIRESNEPISEDTLKTLMNLKEAMCSAKDYLKFCSQGSKIYLVMER 94

Query: 121 HSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERDEALRIR 180
             ++    +++++L   L   P EE+++  ++REQVEL+  Q +RA+  +D  D+ L   
Sbjct: 95  EQVTSKLMEVSVKLEQSLSQIPYEELDISDEVREQVELVLSQFRRAKGRVDVSDDELYED 154

Query: 181 FLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEGD----IEPT 240
                 +  +     P  + E    KL +        E   L E + +  GD    IE  
Sbjct: 155 LQSLCNKSSDVDAYQP--VLERVAKKLHLMEIPDLAQESVALHEMVASSGGDVGENIEEM 214

Query: 241 VAVLYGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFCCPI 300
             VL     F +         E+D  E              +   ++    IP DF CPI
Sbjct: 215 AMVLKMIKDFVQ--------TEDDNGEEQKVGVNSRSNGQTSTAASQKIPVIPDDFRCPI 274

Query: 301 SLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQWCI 360
           SL++M+DPVIVS+GQTY+R+ I +W+E GH TCPKT Q L  T L PN  LR+LI QWC 
Sbjct: 275 SLEMMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRSLIAQWCE 334

Query: 361 AHGV-PYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAAREIR 420
           A+ + P  PP  + P   S + +SP     EAN+     L+ +LA G+ + Q  AA EIR
Sbjct: 335 ANDIEPPKPPSSLRPRKVS-SFSSPA----EANKIED--LMWRLAYGNPEDQRSAAGEIR 394

Query: 421 LLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEVGC 480
           LLAK   +NR  IAEAGAIP L  LL++P+   QE+SVTA+LNLSI + NK  I+S  G 
Sbjct: 395 LLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICENNKGAIVS-AGA 454

Query: 481 LGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRGKK 540
           +  I +VL+ G + EARENAAATLFSLS + + K  I    GA+  L  LL  GT RGKK
Sbjct: 455 IPGIVQVLKKG-SMEARENAAATLFSLSVIDENKVTIGAL-GAIPPLVVLLNEGTQRGKK 514

Query: 541 DAVTALFNLSTHTDNCVRMIEAGAVTALVGAL--GNEGVAEEAAGALALIVRQPVGAAAV 600
           DA TALFNL  +  N  + I AG +  L   L     G+ +EA   LA++   P G A +
Sbjct: 515 DAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSSHPEGKAII 574

Query: 601 GKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLLFT 660
           G  + AV  L+  +R G+PR +ENA A L+ LC SG      +  K   L G L  L   
Sbjct: 575 GSSD-AVPSLVEFIRTGSPRNRENAAAVLVHLC-SGDPQHLVEAQKL-GLMGPLIDLAGN 629

Query: 661 GTKRARRKAASLARVFQR 672
           GT R +RKAA L     R
Sbjct: 635 GTDRGKRKAAQLLERISR 629

BLAST of MC03g0733 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 305.4 bits (781), Expect = 1.2e-82
Identity = 231/640 (36.09%), Postives = 339/640 (52.97%), Query Frame = 0

Query: 44  VSRFSDKAFFFQRRNSRSLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKI 103
           +S FS    F  +     L+RR+ +   F E L D +  L    +   + + + L  S  
Sbjct: 23  ISGFSSSRGFIGKIQG-DLVRRITLLSPFFEELIDVNVELKKDQITGFEAMRIALDSSLE 82

Query: 104 LLDYCSESSKLWLLLQNHSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQL 163
           L    +  SKL+ L    S+   F D+ +E+   L   P E++E+  ++REQV+LL  Q 
Sbjct: 83  LFRSVNGGSKLFQLFDRDSLVEKFRDMTVEIEAALSQIPYEKIEVSEEVREQVQLLHFQF 142

Query: 164 KRARIFIDERDEALRIRFLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLE 223
           KRA+    ER E   ++    L   EN   P+P  ++     +L +      + E   + 
Sbjct: 143 KRAK----ERWEESDLQLSHDLAMAENVMDPDPIILKR-LSQELQLTTIDELKKESHAIH 202

Query: 224 EQIVNHEGD----IEPTVAVLYGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQ 283
           E  ++++GD     E   ++L   V F            E      +T  + + ++    
Sbjct: 203 EYFLSYDGDPDDCFERMSSLLKNLVDFVTM---------ESSDPDPSTGSRIVSRHRSP- 262

Query: 284 EIAETFLTIPRDFCCPISLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHT 343
                   IP  F CPISL+LMKDPVIVSTGQTY+RSSI +W++ GH TCPK+ + L+H 
Sbjct: 263 -------VIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDAGHKTCPKSQETLLHA 322

Query: 344 RLAPNRALRNLIMQWCIAHGVPYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQL 403
            L PN  L++LI  WC ++G+     +G   + +   ++S      + +R     L+++L
Sbjct: 323 GLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSS-----DCDRTFVLSLLEKL 382

Query: 404 AIGSQDAQTIAAREIRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNL 463
           A G+ + Q  AA E+RLLAK   +NR  IAEAGAIP L +LL+SP+P  QE+SVTA+LNL
Sbjct: 383 ANGTTEQQRAAAGELRLLAKRNVDNRVCIAEAGAIPLLVELLSSPDPRTQEHSVTALLNL 442

Query: 464 SIYDKNKSLIMSEVGCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAV 523
           SI + NK  I+ + G +  I EVL+ G + EARENAAATLFSLS + + K  I    GA+
Sbjct: 443 SINEGNKGAIV-DAGAITDIVEVLKNG-SMEARENAAATLFSLSVIDENKVAIG-AAGAI 502

Query: 524 EALAGLLRNGTPRGKKDAVTALFNLSTHTDNCVRMIEAGAVTALVGAL--GNEGVAEEAA 583
           +AL  LL  GT RGKKDA TA+FNL  +  N  R ++ G V  L   L     G+ +EA 
Sbjct: 503 QALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPLTRLLKDAGGGMVDEAL 562

Query: 584 GALALIVRQPVGAAAVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQV 643
             LA++     G  A+ + E ++  L+ ++R G+PR +ENA A L  LC   G      V
Sbjct: 563 AILAILSTNQEGKTAIAEAE-SIPVLVEIIRTGSPRNRENAAAILWYLCI--GNIERLNV 622

Query: 644 FKAPALAGLLQTLLFTGTKRARRKAASLARVFQRCEHVAM 678
            +       L+ L   GT RA+RKAASL  + Q+ E VA+
Sbjct: 623 AREVGADVALKELTENGTDRAKRKAASLLELIQQTEGVAV 628

BLAST of MC03g0733 vs. TAIR 10
Match: AT5G42340.1 (Plant U-Box 15 )

HSP 1 Score: 280.8 bits (717), Expect = 3.1e-75
Identity = 210/627 (33.49%), Postives = 340/627 (54.23%), Query Frame = 0

Query: 55  QRRNSRSLIRRLQVCLVFLEFLKDSDATLPYTALLCLKELYLLLYRSKILLDYCSESSKL 114
           Q++   +L+RRL++ + FL+ ++  ++      L  L++++L    +K LL+ CS  SK+
Sbjct: 74  QQKECFNLVRRLKILIPFLDEIRGFESPSCKHFLNRLRKVFL---AAKKLLETCSNGSKI 133

Query: 115 WLLLQNHSISGHFNDLNLELLTFLDVFPLEEVELGVDIREQVELLQKQLKRARIFIDERD 174
           ++ L   ++   F+ +  +L   L   P +E+ +  D +++++ L KQLK+A+   D +D
Sbjct: 134 YMALDGETMMTRFHSIYEKLNRVLVKAPFDELMISGDAKDEIDSLCKQLKKAKRRTDTQD 193

Query: 175 EALRIRFLLFLEEFENGRLPNPREMREFFVDKLGIRNAKSCRAEIEFLEEQIVNHEG-DI 234
             L +  ++   + +     +   + E    KL ++     + E   ++  I +  G +I
Sbjct: 194 IELAVDMMVVFSKTDPRNADS--AIIERLAKKLELQTIDDLKTETIAIQSLIQDKGGLNI 253

Query: 235 EPTVAVLYGFVAFTRYSRFFLFGFEEDEIESGATNQKKLKKNLITQEIAETFLTIPRDFC 294
           E    ++     F +     L G E  +I       K + K+        T L +P +F 
Sbjct: 254 ETKQHIIELLNKFKK-----LQGLEATDILYQPVINKAITKS--------TSLILPHEFL 313

Query: 295 CPISLDLMKDPVIVSTGQTYDRSSIVRWMEEGHCTCPKTGQMLIHTRLAPNRALRNLIMQ 354
           CPI+L++M DPVI++TGQTY++ SI +W + GH TCPKT Q L H  LAPN AL+NLIMQ
Sbjct: 314 CPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKTCPKTRQELDHLSLAPNFALKNLIMQ 373

Query: 355 WCIAHGVPYDPPEGMDPSAESFATASPTRAALEANRATAAILIQQLAIGSQDAQTIAARE 414
           WC  +     P + + P +++              +   ++L++ L+    + Q  + ++
Sbjct: 374 WCEKNNFKI-PEKEVSPDSQN------------EQKDEVSLLVEALSSSQLEEQRRSVKQ 433

Query: 415 IRLLAKTGKENRAFIAEAGAIPHLQKLLASPNPVAQENSVTAMLNLSIYDKNKSLIMSEV 474
           +RLLA+   ENR  IA AGAIP L +LL+ P+   QEN+VT +LNLSI + NK LI +E 
Sbjct: 434 MRLLARENPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLNLSIDEVNKKLISNE- 493

Query: 475 GCLGSITEVLRFGHTTEARENAAATLFSLSAVHDFKKRIAEEGGAVEALAGLLRNGTPRG 534
           G + +I E+L  G+  EAREN+AA LFSLS + + K  I    G +  L  LL++GT RG
Sbjct: 494 GAIPNIIEILENGN-REARENSAAALFSLSMLDENKVTIGLSNG-IPPLVDLLQHGTLRG 553

Query: 535 KKDAVTALFNLSTHTDNCVRMIEAGAVTALVGAL--GNEGVAEEAAGALALIVRQPVGAA 594
           KKDA+TALFNLS ++ N  R I+AG V  L+  L   N G+ +EA   L L+   P G  
Sbjct: 554 KKDALTALFNLSLNSANKGRAIDAGIVQPLLNLLKDKNLGMIDEALSILLLLASHPEGRQ 613

Query: 595 AVGKEERAVAGLIAMMRCGTPRGKENAVAALLELCRSGGAATTEQVFKAPALAGLLQTLL 654
           A+G +   +  L+  +R GTP+ KE A + LLEL      +       A    G+ + L+
Sbjct: 614 AIG-QLSFIETLVEFIRQGTPKNKECATSVLLEL-----GSNNSSFILAALQFGVYEYLV 660

Query: 655 ---FTGTKRARRKAASLARVFQRCEHV 676
               +GT RA+RKA +L ++  + E +
Sbjct: 674 EITTSGTNRAQRKANALIQLISKSEQI 660

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C7R65.3e-26668.31U-box domain-containing protein 17 OS=Arabidopsis thaliana OX=3702 GN=PUB17 PE=2... [more]
Q6EUK71.8e-14946.14U-box domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PU... [more]
E4NKF85.2e-10437.76U-box domain-containing protein 1 OS=Medicago truncatula OX=3880 GN=PUB1 PE=1 SV... [more]
Q9LZW37.6e-9536.32U-box domain-containing protein 16 OS=Arabidopsis thaliana OX=3702 GN=PUB16 PE=2... [more]
Q9SNC61.3e-8337.70U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Match NameE-valueIdentityDescription
XP_022140088.10.0100.00U-box domain-containing protein 17 [Momordica charantia][more]
XP_008443533.10.091.89PREDICTED: U-box domain-containing protein 17 [Cucumis melo] >KAA0057064.1 U-box... [more]
XP_004147388.10.091.47U-box domain-containing protein 17 [Cucumis sativus] >KGN65604.1 hypothetical pr... [more]
XP_038894534.10.091.47U-box domain-containing protein 17 [Benincasa hispida][more]
KAG6583941.10.089.93U-box domain-containing protein 17, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
A0A6J1CH750.0100.00RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101082... [more]
A0A5A7UPD80.091.89RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3B9120.091.89RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487100 PE=4 ... [more]
A0A0A0LX590.091.47RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G467720 PE... [more]
A0A6J1EI410.089.93RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434297... [more]
Match NameE-valueIdentityDescription
AT1G29340.13.8e-26768.31plant U-box 17 [more]
AT5G01830.15.4e-9636.32ARM repeat superfamily protein [more]
AT3G46510.19.5e-8537.70plant U-box 13 [more]
AT3G54850.11.2e-8236.09plant U-box 14 [more]
AT5G42340.13.1e-7533.49Plant U-Box 15 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 212..232
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 11..693
NoneNo IPR availablePANTHERPTHR23315:SF266U-BOX DOMAIN-CONTAINING PROTEIN 17coord: 11..693
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 292..334
e-value: 9.31758E-27
score: 100.771
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 277..362
IPR000225ArmadilloSMARTSM00185arm_5coord: 547..585
e-value: 0.0087
score: 25.3
coord: 506..546
e-value: 5.3
score: 15.4
coord: 421..461
e-value: 0.36
score: 19.9
IPR000225ArmadilloPFAMPF00514Armcoord: 422..460
e-value: 8.5E-6
score: 25.6
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 517..559
score: 8.7674
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 432..474
score: 10.1674
IPR003613U box domainSMARTSM00504Ubox_2coord: 291..354
e-value: 9.5E-30
score: 114.8
IPR003613U box domainPFAMPF04564U-boxcoord: 288..358
e-value: 5.7E-19
score: 68.1
IPR003613U box domainPROSITEPS51698U_BOXcoord: 287..361
score: 37.074093
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 391..512
e-value: 4.2E-19
score: 70.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 513..667
e-value: 6.8E-19
score: 70.1
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 287..362
e-value: 2.9E-27
score: 96.5
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 394..674

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0733.1MC03g0733.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007166 cell surface receptor signaling pathway
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity