MC03g0659 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0659
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	endochitinase EP3-like
Location	MC03: 13551814 .. 13553030 (-)
RNA-Seq Expression	MC03g0659
Synteny	MC03g0659

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCGCAGCAGTTCCGTATAAATACCCTTCCGTCTTCTCCTCAGTCTCACAATTCTCCCTCCCATCGCTCTTCTTCTTCTTCACTTCACCAAATTCCCCCCTCAGAAAATTAAAAATGGGTGCTGCGGCGGCGAAAAGGTCGCTGCTGCTAGCGGCGGCTCTGATTATCGGAGTAATCGGGGGAGCGGCGGCGCAGAACTGCGGGTGCGCGGCGGGGCTGTGCTGCAGCAGGTTCGGATTCTGCGGGACCGGAGAAGATTTCTGCGGGAGCGGGTGCAGGGAGGGGCCTTGCAACATTCCGCCGCTGAGCCCTAGCGTGAACGACGTCGTCGTGGCTGATTTCGTGACGGAGGAGTTCTTCAACGGTATAATTAACCAGGCCGACGCGGGGTGCGCCGGACGGGGATTCTACAGCAGAGCAACTTTTCTTCAGGCTCTCCAATCGTTCGATAGATTTGGACGGATTGGATCTGTTGACGATTCTAAAAGAGAAATTGCCGCCTTCTTCGGCCACGTCACTCACGAGACCGGACGTAAGCTTCGTTTCATAAAAGTATTTTATTTTGTGTTTTTTGCTTTTCAGCTTCTTGAAAGTATGTACAAAATTTCTGAAAGTTTAGAAACTATAGAATAAATAAATAATTAACAAGAAACTAATTAAGAAACACTAAAATAATTTCCCTGCAAAGTTAATGAGCTAAAAGAATAGCATTTGAAAGTGAAGAAACAAATAATGGTTCATCAAAGATAAATTTGATTAAACTTAAAATGCCTTATTAAGTAATTTTATTATATTTTTTTTGTTGGAACATAGATTTTTGCTTCATAGAAGAAATAAATGGGCCATCGGGGGACTACTGTGACGAGGAAAATACCCAATACCCATGCAACCCGAGCAAGGGGTATTACGGGCGCGGCCCGATCCAGCTATCGTGGAACTTCAACTACGGTCCGGCCGGAGAGGCCATCGGGTTCGACGGTCTAAACAACCCGGAAATCGTCGCAACCGACCCGGTCGTCTCCTTCAAGACGGCGCTGTGGTACTGGATGAGCTTCGTCCGACCCGTTATCAACCAGGGCTTCGGAGCCACCATTCGGGCCATTAACGGCGCGCTCGAGTGCGACGGCGGTAACTCCGCCACCGTCCAACGCCGGATCCAATTCTACACTCAGTACTGTAACCAACTTGGAGTTGCCCCGGGAGATAATTTGACATGT

mRNA sequence

CGCGCAGCAGTTCCGTATAAATACCCTTCCGTCTTCTCCTCAGTCTCACAATTCTCCCTCCCATCGCTCTTCTTCTTCTTCACTTCACCAAATTCCCCCCTCAGAAAATTAAAAATGGGTGCTGCGGCGGCGAAAAGGTCGCTGCTGCTAGCGGCGGCTCTGATTATCGGAGTAATCGGGGGAGCGGCGGCGCAGAACTGCGGGTGCGCGGCGGGGCTGTGCTGCAGCAGGTTCGGATTCTGCGGGACCGGAGAAGATTTCTGCGGGAGCGGGTGCAGGGAGGGGCCTTGCAACATTCCGCCGCTGAGCCCTAGCGTGAACGACGTCGTCGTGGCTGATTTCGTGACGGAGGAGTTCTTCAACGGTATAATTAACCAGGCCGACGCGGGGTGCGCCGGACGGGGATTCTACAGCAGAGCAACTTTTCTTCAGGCTCTCCAATCGTTCGATAGATTTGGACGGATTGGATCTGTTGACGATTCTAAAAGAGAAATTGCCGCCTTCTTCGGCCACGTCACTCACGAGACCGGACTAATTTTATTATATTTTTTTTGTTGGAACATAGATTTTTGCTTCATAGAAGAAATAAATGGGCCATCGGGGGACTACTGTGACGAGGAAAATACCCAATACCCATGCAACCCGAGCAAGGGGTATTACGGGCGCGGCCCGATCCAGCTATCGTGGAACTTCAACTACGGTCCGGCCGGAGAGGCCATCGGGTTCGACGGTCTAAACAACCCGGAAATCGTCGCAACCGACCCGGTCGTCTCCTTCAAGACGGCGCTGTGGTACTGGATGAGCTTCGTCCGACCCGTTATCAACCAGGGCTTCGGAGCCACCATTCGGGCCATTAACGGCGCGCTCGAGTGCGACGGCGGTAACTCCGCCACCGTCCAACGCCGGATCCAATTCTACACTCAGTACTGTAACCAACTTGGAGTTGCCCCGGGAGATAATTTGACATGT

Coding sequence (CDS)

CGCGCAGCAGTTCCGTATAAATACCCTTCCGTCTTCTCCTCAGTCTCACAATTCTCCCTCCCATCGCTCTTCTTCTTCTTCACTTCACCAAATTCCCCCCTCAGAAAATTAAAAATGGGTGCTGCGGCGGCGAAAAGGTCGCTGCTGCTAGCGGCGGCTCTGATTATCGGAGTAATCGGGGGAGCGGCGGCGCAGAACTGCGGGTGCGCGGCGGGGCTGTGCTGCAGCAGGTTCGGATTCTGCGGGACCGGAGAAGATTTCTGCGGGAGCGGGTGCAGGGAGGGGCCTTGCAACATTCCGCCGCTGAGCCCTAGCGTGAACGACGTCGTCGTGGCTGATTTCGTGACGGAGGAGTTCTTCAACGGTATAATTAACCAGGCCGACGCGGGGTGCGCCGGACGGGGATTCTACAGCAGAGCAACTTTTCTTCAGGCTCTCCAATCGTTCGATAGATTTGGACGGATTGGATCTGTTGACGATTCTAAAAGAGAAATTGCCGCCTTCTTCGGCCACGTCACTCACGAGACCGGACTAATTTTATTATATTTTTTTTGTTGGAACATAGATTTTTGCTTCATAGAAGAAATAAATGGGCCATCGGGGGACTACTGTGACGAGGAAAATACCCAATACCCATGCAACCCGAGCAAGGGGTATTACGGGCGCGGCCCGATCCAGCTATCGTGGAACTTCAACTACGGTCCGGCCGGAGAGGCCATCGGGTTCGACGGTCTAAACAACCCGGAAATCGTCGCAACCGACCCGGTCGTCTCCTTCAAGACGGCGCTGTGGTACTGGATGAGCTTCGTCCGACCCGTTATCAACCAGGGCTTCGGAGCCACCATTCGGGCCATTAACGGCGCGCTCGAGTGCGACGGCGGTAACTCCGCCACCGTCCAACGCCGGATCCAATTCTACACTCAGTACTGTAACCAACTTGGAGTTGCCCCGGGAGATAATTTGACATGT

Protein sequence

RAAVPYKYPSVFSSVSQFSLPSLFFFFTSPNSPLRKLKMGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC

Homology

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BLAST of MC03g0659 vs. ExPASy Swiss-Prot
Match: Q9M2U5 (Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 6.4e-110
Identity = 183/260 (70.38%), Postives = 209/260 (80.38%), Query Frame = 0


Query: 64  AQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSPSVNDVVVADFVTEEFFNGI 123
           AQNCGC++ LCCS+FGFCG   D+CG GC++GPC  P   P  N V VA+ VT+EFFNGI
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAP---PPANGVSVAEIVTQEFFNGI 87

Query: 124 INQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGLILLYF 183
           I+QA + CAG  FYSR  FL+AL S+ RFGR+GS DDS+REIAAFF HVTHETG      
Sbjct: 88  ISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETG------ 147

Query: 184 FCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFD 243
                 FC+IEEI+G S DYCDE  TQYPCNP+KGYYGRGPIQLSWNFNYGPAG AIGFD
Sbjct: 148 -----HFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFD 207

Query: 244 GLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRAINGALECDGGNSATVQRRI 303
           GLN PE VATDPV+SFKTALWYW + V+PVI+QGFGATIRAINGALECDG N+ATVQ R+
Sbjct: 208 GLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARV 267

Query: 304 QFYTQYCNQLGVAPGDNLTC 324
           ++YT YC QLGV PG+NLTC
Sbjct: 268 RYYTDYCRQLGVDPGNNLTC 273

[Close]

BLAST of MC03g0659 vs. ExPASy Swiss-Prot
Match: P27054 (Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-104
Identity = 183/277 (66.06%), Postives = 212/277 (76.53%), Query Frame = 0


Query: 48  LLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSPSVN 107
           +L+A AL++G     +AQNCGCA GLCCS++G+CGTGED+CG+GC++GPC      PS N
Sbjct: 8   VLVAVALVMGP-KNVSAQNCGCAEGLCCSQYGYCGTGEDYCGTGCQQGPCTTASPPPSNN 67

Query: 108 DVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAA 167
             V AD +T +F NGII+QAD+GCAG+ FY+R  FL AL S+  FGR+GS DDSKREIAA
Sbjct: 68  --VNADILTADFLNGIIDQADSGCAGKNFYTRDAFLSALNSYTDFGRVGSEDDSKREIAA 127

Query: 168 FFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEEN-TQYPCNPSKGYYGRGPIQ 227
            F H THETG            FC+IEEI+G S DYCDEE+  QYPC+ SKGY+GRGPIQ
Sbjct: 128 AFAHFTHETG-----------HFCYIEEIDGASKDYCDEESIAQYPCSSSKGYHGRGPIQ 187

Query: 228 LSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRAIN 287
           LSWNFNYGPAG A  FDGL  PE V+ D VVSFKTALWYWM  VRPVINQGFGATIRAIN
Sbjct: 188 LSWNFNYGPAGSANNFDGLGAPETVSNDVVVSFKTALWYWMQHVRPVINQGFGATIRAIN 247

Query: 288 GALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           GALECDG N  TVQ R+ +YT+YC QLGVA GDNLTC
Sbjct: 248 GALECDGANPTTVQARVNYYTEYCRQLGVATGDNLTC 270

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BLAST of MC03g0659 vs. ExPASy Swiss-Prot
Match: Q6K8R2 (Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.9e-94
Identity = 164/285 (57.54%), Postives = 201/285 (70.53%), Query Frame = 0


Query: 43  AAKRSLLLAAALIIGVIGG--AAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIP 102
           A + SLL     ++  +    AAAQ+CGCA+  CCS++GFCGTG D+CG+GC+ GPC++ 
Sbjct: 2   ARRLSLLAVVLAMVAAVSASTAAAQSCGCASDQCCSKWGFCGTGSDYCGTGCQAGPCDV- 61

Query: 103 PLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDD 162
              P+ NDV VA  VT EFF  ++ QAD GCA +GFY+R  FL A   +  FGR GSVDD
Sbjct: 62  ---PATNDVSVASIVTPEFFAALVAQADDGCAAKGFYTRDAFLTAAGGYPSFGRTGSVDD 121

Query: 163 SKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYY 222
           SKREIAAFF H  HET           I FC+IEEI+GPS +YCDE +TQ+PC   KGYY
Sbjct: 122 SKREIAAFFAHANHET-----------IKFCYIEEIDGPSKNYCDETSTQWPCMAGKGYY 181

Query: 223 GRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRP--VINQGF 282
           GRGP+Q+SWNFNYGPAG++IGFDGL +P+ VA  PV++F+TALWYW + V    V  QGF
Sbjct: 182 GRGPLQISWNFNYGPAGQSIGFDGLGDPDAVARSPVLAFQTALWYWTNNVHDAFVSGQGF 241

Query: 283 GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           GATIRAINGALECDG N   V  R+ +Y Q+C Q GV PG NLTC
Sbjct: 242 GATIRAINGALECDGKNPTAVSNRVAYYQQFCQQFGVDPGSNLTC 271

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BLAST of MC03g0659 vs. ExPASy Swiss-Prot
Match: Q7Y1Z0 (Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 4.3e-90
Identity = 159/287 (55.40%), Postives = 191/287 (66.55%), Query Frame = 0


Query: 48  LLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPC---------- 107
           L L  AL++   G A+AQNCGC + +CCS++G+CGTG+D+CG GCR GPC          
Sbjct: 13  LALGLALLLSATGQASAQNCGCQSNMCCSKWGYCGTGKDYCGDGCRSGPCYGGGGGGGGG 72

Query: 108 -NIPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIG 167
                     + V V   VTE FFNGI NQA  GCAG+ FY+R +FL A  S+  F R  
Sbjct: 73  GGGGGGGGGGSGVSVESVVTEAFFNGIKNQAPNGCAGKNFYTRQSFLNAAHSYSGFARDR 132

Query: 168 SVDDSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPS 227
           + DDSKREIAAFF HVTHETG             C+I EING S DYCD+ N Q+PC P 
Sbjct: 133 TNDDSKREIAAFFAHVTHETG-----------HMCYINEINGASMDYCDKNNKQWPCQPG 192

Query: 228 KGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQ 287
           K YYGRGP+Q+SWN+NYGPAG+ IGFDGL +P+ VA DP +SFKTALW+WM+ V  V+ Q
Sbjct: 193 KKYYGRGPLQISWNYNYGPAGQNIGFDGLRDPDRVAQDPTISFKTALWFWMNNVHQVMLQ 252

Query: 288 GFGATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           GFGATIRAINGALEC+G N   V  R+ +Y  YC Q GV PG NL C
Sbjct: 253 GFGATIRAINGALECNGKNPGAVNARVNYYKDYCRQFGVDPGGNLYC 288

[Close]

BLAST of MC03g0659 vs. ExPASy Swiss-Prot
Match: O04138 (Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2)

HSP 1 Score: 326.6 bits (836), Expect = 3.1e-88
Identity = 156/287 (54.36%), Postives = 191/287 (66.55%), Query Frame = 0


Query: 47  SLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPC--------- 106
           +L+   AL++     AAAQNCGC  G CCS++G+CGT E +CG GC+ GPC         
Sbjct: 10  ALVFGLALLLSAAAPAAAQNCGCQDGYCCSQWGYCGTTEAYCGQGCQSGPCWGSGGEAAA 69

Query: 107 -NIPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIG 166
                 + +   V V   VTE FFNGI NQA  GCAG+ FY+R +FL A +S+  F    
Sbjct: 70  GMAGRKAGAGAGVSVESVVTEAFFNGIKNQAPNGCAGKSFYTRQSFLNAARSYSGFANDR 129

Query: 167 SVDDSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPS 226
           + DDSKREIAAFF HVTHETG             C+I EING + DYCD+ N Q+PC P 
Sbjct: 130 TNDDSKREIAAFFAHVTHETG-----------HMCYINEINGANMDYCDKSNKQWPCQPG 189

Query: 227 KGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQ 286
           K YYGRGP+Q+SWNFNYGPAG+ IGFDGL +P+ VA DP +SFKTALW+WM+ V  V++Q
Sbjct: 190 KKYYGRGPLQISWNFNYGPAGKNIGFDGLRDPDKVAQDPTISFKTALWFWMNNVHQVMSQ 249

Query: 287 GFGATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           GFGATIRAINGALEC+G N   V  R+ +Y  YC Q GV+PG NL C
Sbjct: 250 GFGATIRAINGALECNGKNPGAVNARVNYYKDYCRQFGVSPGGNLYC 285

[Close]

BLAST of MC03g0659 vs. NCBI nr
Match: XP_022140102.1 (endochitinase EP3-like [Momordica charantia])

HSP 1 Score: 563 bits (1450), Expect = 1.27e-201
Identity = 273/285 (95.79%), Postives = 273/285 (95.79%), Query Frame = 0


Query: 39  MGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCN 98
           MGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCN
Sbjct: 1   MGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCN 60

Query: 99  IPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSV 158
           IPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSV
Sbjct: 61  IPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSV 120

Query: 159 DDSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKG 218
           DDSKREIAAFFGHVTHETG            FCFIEEINGPSGDYCDEENTQYPCNPSKG
Sbjct: 121 DDSKREIAAFFGHVTHETG-----------HFCFIEEINGPSGDYCDEENTQYPCNPSKG 180

Query: 219 YYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGF 278
           YYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGF
Sbjct: 181 YYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGF 240

Query: 279 GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC
Sbjct: 241 GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 274

[Close]

BLAST of MC03g0659 vs. NCBI nr
Match: XP_023001456.1 (endochitinase EP3 [Cucurbita maxima])

HSP 1 Score: 511 bits (1315), Expect = 3.80e-181
Identity = 241/279 (86.38%), Postives = 259/279 (92.83%), Query Frame = 0


Query: 45  KRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSP 104
           +R+LLL   L+ G+I GAAAQNCGCAAGLCCSRFGFCGTG DFCG+GCREGPCNIPPL+P
Sbjct: 2   QRALLLTVTLV-GIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLTP 61

Query: 105 SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKRE 164
           SVNDVVVADFVTEEFFNGI+NQADAGCAGRGFYSRATFL+ALQSFDRFGRIGSVDDSKRE
Sbjct: 62  SVNDVVVADFVTEEFFNGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKRE 121

Query: 165 IAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGP 224
           IAAFFGHVTHETG +           CFIEEI+G + DYCDEENTQYPCNPSKGY+GRGP
Sbjct: 122 IAAFFGHVTHETGHL-----------CFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGP 181

Query: 225 IQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRA 284
           IQLSWNFNYGPAGE+IGFDGLNNPEIVAT+PVVSFKTALWYWM+FVRPVINQGFGATIRA
Sbjct: 182 IQLSWNFNYGPAGESIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRA 241

Query: 285 INGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           INGALECDGGNSATVQRR+++YTQYCNQLGVAPGDNLTC
Sbjct: 242 INGALECDGGNSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of MC03g0659 vs. NCBI nr
Match: XP_038894874.1 (endochitinase EP3-like [Benincasa hispida])

HSP 1 Score: 511 bits (1315), Expect = 4.92e-181
Identity = 241/284 (84.86%), Postives = 260/284 (91.55%), Query Frame = 0


Query: 40  GAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNI 99
           G  A +++LL+ A  +IG+I GAAAQNCGCAAGLCCSRFGFCG GEDFCG+GCREGPCNI
Sbjct: 3   GGVAMQKTLLITAVALIGIITGAAAQNCGCAAGLCCSRFGFCGNGEDFCGTGCREGPCNI 62

Query: 100 PPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVD 159
           PPL+PSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFL+AL+SFDRFGRIGSVD
Sbjct: 63  PPLTPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALRSFDRFGRIGSVD 122

Query: 160 DSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGY 219
           DSKREIAAFFGHVTHETG +           CFIEEI+G + DYCDEENTQYPCNPSKGY
Sbjct: 123 DSKREIAAFFGHVTHETGHL-----------CFIEEIDGATKDYCDEENTQYPCNPSKGY 182

Query: 220 YGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFG 279
           +GRGPIQLSWNFNYGPAGE IGFDGLNNPEIVATDPVVSFKTALWYWM+FVRPVINQGFG
Sbjct: 183 FGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATDPVVSFKTALWYWMNFVRPVINQGFG 242

Query: 280 ATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           ATIRAINGALECDG N+ATVQRR+QFYTQYC+QLGVAPG+NLTC
Sbjct: 243 ATIRAINGALECDGANNATVQRRVQFYTQYCDQLGVAPGNNLTC 275

[Close]

BLAST of MC03g0659 vs. NCBI nr
Match: XP_023520063.1 (endochitinase EP3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509 bits (1310), Expect = 2.20e-180
Identity = 240/279 (86.02%), Postives = 259/279 (92.83%), Query Frame = 0


Query: 45  KRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSP 104
           +R+LLL   L+ G+I GAAAQNCGCAAGLCCSRFGFCGTG+DFCG+GCREGPCNIPPL+P
Sbjct: 2   QRALLLTVTLV-GIIAGAAAQNCGCAAGLCCSRFGFCGTGDDFCGTGCREGPCNIPPLTP 61

Query: 105 SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKRE 164
           SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFL+ALQSFDRFGRIGSVDDSKRE
Sbjct: 62  SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKRE 121

Query: 165 IAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGP 224
           IAAFFGHVTHETG +           CFIEEI+G + DYCDEENTQYPCNP+KGY+GRGP
Sbjct: 122 IAAFFGHVTHETGHL-----------CFIEEIDGATKDYCDEENTQYPCNPNKGYFGRGP 181

Query: 225 IQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRA 284
           IQLSWNFNYGPAGE+IGFDGLNNPEIVAT+PVVSFKTALWYWM+FVRPVINQGFGATIRA
Sbjct: 182 IQLSWNFNYGPAGESIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRA 241

Query: 285 INGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           INGALECDG NSATVQRR+++YTQYCNQLGVAPGDNLTC
Sbjct: 242 INGALECDGANSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of MC03g0659 vs. NCBI nr
Match: KAG7019501.1 (Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 508 bits (1308), Expect = 4.43e-180
Identity = 240/279 (86.02%), Postives = 258/279 (92.47%), Query Frame = 0


Query: 45  KRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSP 104
           +R+LLL   L+ G+I GAAAQNCGCAAGLCCSRFGFCGTG DFCG+GCREGPCNIPPL+P
Sbjct: 2   QRALLLTVTLV-GIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLTP 61

Query: 105 SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKRE 164
           SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFL+ALQSFDRFGRIGSVDDSKRE
Sbjct: 62  SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKRE 121

Query: 165 IAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGP 224
           IAAFFGHVTHETG +           CFIEEI+G + DYCDEENTQYPCNP+KGY+GRGP
Sbjct: 122 IAAFFGHVTHETGHL-----------CFIEEIDGATKDYCDEENTQYPCNPNKGYFGRGP 181

Query: 225 IQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRA 284
           IQLSWNFNYGPAGE+IGFDGLNNPEIVAT+P+VSFKTALWYWM+FVRPVINQGFGATIRA
Sbjct: 182 IQLSWNFNYGPAGESIGFDGLNNPEIVATNPLVSFKTALWYWMNFVRPVINQGFGATIRA 241

Query: 285 INGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           INGALECDG NSATVQRR+Q+YTQYCNQLGVAPGDNLTC
Sbjct: 242 INGALECDGANSATVQRRVQYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of MC03g0659 vs. ExPASy TrEMBL
Match: A0A6J1CFV8 (endochitinase EP3-like OS=Momordica charantia OX=3673 GN=LOC111010839 PE=4 SV=1)

HSP 1 Score: 563 bits (1450), Expect = 6.16e-202
Identity = 273/285 (95.79%), Postives = 273/285 (95.79%), Query Frame = 0


Query: 39  MGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCN 98
           MGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCN
Sbjct: 1   MGAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCN 60

Query: 99  IPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSV 158
           IPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSV
Sbjct: 61  IPPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSV 120

Query: 159 DDSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKG 218
           DDSKREIAAFFGHVTHETG            FCFIEEINGPSGDYCDEENTQYPCNPSKG
Sbjct: 121 DDSKREIAAFFGHVTHETG-----------HFCFIEEINGPSGDYCDEENTQYPCNPSKG 180

Query: 219 YYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGF 278
           YYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGF
Sbjct: 181 YYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGF 240

Query: 279 GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC
Sbjct: 241 GATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 274

[Close]

BLAST of MC03g0659 vs. ExPASy TrEMBL
Match: A0A6J1KIN5 (endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1)

HSP 1 Score: 511 bits (1315), Expect = 1.84e-181
Identity = 241/279 (86.38%), Postives = 259/279 (92.83%), Query Frame = 0


Query: 45  KRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSP 104
           +R+LLL   L+ G+I GAAAQNCGCAAGLCCSRFGFCGTG DFCG+GCREGPCNIPPL+P
Sbjct: 2   QRALLLTVTLV-GIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLTP 61

Query: 105 SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKRE 164
           SVNDVVVADFVTEEFFNGI+NQADAGCAGRGFYSRATFL+ALQSFDRFGRIGSVDDSKRE
Sbjct: 62  SVNDVVVADFVTEEFFNGILNQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKRE 121

Query: 165 IAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGP 224
           IAAFFGHVTHETG +           CFIEEI+G + DYCDEENTQYPCNPSKGY+GRGP
Sbjct: 122 IAAFFGHVTHETGHL-----------CFIEEIDGATKDYCDEENTQYPCNPSKGYFGRGP 181

Query: 225 IQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRA 284
           IQLSWNFNYGPAGE+IGFDGLNNPEIVAT+PVVSFKTALWYWM+FVRPVINQGFGATIRA
Sbjct: 182 IQLSWNFNYGPAGESIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFGATIRA 241

Query: 285 INGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           INGALECDGGNSATVQRR+++YTQYCNQLGVAPGDNLTC
Sbjct: 242 INGALECDGGNSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of MC03g0659 vs. ExPASy TrEMBL
Match: A0A6J1EGZ6 (endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1)

HSP 1 Score: 507 bits (1305), Expect = 6.15e-180
Identity = 239/279 (85.66%), Postives = 258/279 (92.47%), Query Frame = 0


Query: 45  KRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSP 104
           +R+LLL   L+ G+I GAAAQNCGCAAGLCCSRFGFCGTG DFCG+GCREGPCNIPPL+P
Sbjct: 2   QRALLLTVTLV-GIIAGAAAQNCGCAAGLCCSRFGFCGTGNDFCGTGCREGPCNIPPLTP 61

Query: 105 SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKRE 164
           SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFL+ALQSFDRFGRIGSVDDSKRE
Sbjct: 62  SVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLEALQSFDRFGRIGSVDDSKRE 121

Query: 165 IAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGP 224
           IAAFFGHVTHETG +           CFIEEI+G + DYCDE+NTQYPCNPSKGY+GRGP
Sbjct: 122 IAAFFGHVTHETGHL-----------CFIEEIDGATKDYCDEDNTQYPCNPSKGYFGRGP 181

Query: 225 IQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRA 284
           IQLSWNFNYGPAGE+IGFDGLNNPEIVAT+P+VSFKTALWYWM+FVRPVINQGFGATIRA
Sbjct: 182 IQLSWNFNYGPAGESIGFDGLNNPEIVATNPLVSFKTALWYWMNFVRPVINQGFGATIRA 241

Query: 285 INGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           INGALECDG NSATVQRR+++YTQYCNQLGVAPGDNLTC
Sbjct: 242 INGALECDGANSATVQRRVEYYTQYCNQLGVAPGDNLTC 268

[Close]

BLAST of MC03g0659 vs. ExPASy TrEMBL
Match: A0A5A7UFI0 (Endochitinase EP3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00900 PE=4 SV=1)

HSP 1 Score: 501 bits (1291), Expect = 1.08e-177
Identity = 237/284 (83.45%), Postives = 255/284 (89.79%), Query Frame = 0


Query: 40  GAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNI 99
           G  A +++LLL A  +IG+I GAAAQNCGCAAGLCCSRFGFCGTGEDFCG+GCREGPCNI
Sbjct: 3   GGVAMQKTLLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCREGPCNI 62

Query: 100 PPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVD 159
           PPL+PSVNDV VA+FVTEEFFNGIINQADA CAGRGFYSRATFL+ALQSFDRFGRIG+VD
Sbjct: 63  PPLTPSVNDVNVAEFVTEEFFNGIINQADASCAGRGFYSRATFLEALQSFDRFGRIGTVD 122

Query: 160 DSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGY 219
           DSKREIAAFF HVTHETG            FCFIEEI+G + DYCDEENTQYPCNPSKGY
Sbjct: 123 DSKREIAAFFAHVTHETG-----------HFCFIEEIDGATRDYCDEENTQYPCNPSKGY 182

Query: 220 YGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFG 279
           +GRGPIQLSWNFNYGPAGE IGFDGLNNPEIVAT+PVVSFKTALWYWM+FVRPVINQGFG
Sbjct: 183 FGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFG 242

Query: 280 ATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           ATIRAINGALECDG N+ATVQRR+QFY QYCNQL V PG+NLTC
Sbjct: 243 ATIRAINGALECDGANTATVQRRVQFYNQYCNQLNVDPGNNLTC 275

[Close]

BLAST of MC03g0659 vs. ExPASy TrEMBL
Match: A0A1S3BAS2 (endochitinase EP3-like OS=Cucumis melo OX=3656 GN=LOC103487878 PE=4 SV=1)

HSP 1 Score: 501 bits (1291), Expect = 1.08e-177
Identity = 237/284 (83.45%), Postives = 255/284 (89.79%), Query Frame = 0


Query: 40  GAAAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNI 99
           G  A +++LLL A  +IG+I GAAAQNCGCAAGLCCSRFGFCGTGEDFCG+GCREGPCNI
Sbjct: 3   GGVAMQKTLLLTAVALIGIIAGAAAQNCGCAAGLCCSRFGFCGTGEDFCGTGCREGPCNI 62

Query: 100 PPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVD 159
           PPL+PSVNDV VA+FVTEEFFNGIINQADA CAGRGFYSRATFL+ALQSFDRFGRIG+VD
Sbjct: 63  PPLTPSVNDVNVAEFVTEEFFNGIINQADASCAGRGFYSRATFLEALQSFDRFGRIGTVD 122

Query: 160 DSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGY 219
           DSKREIAAFF HVTHETG            FCFIEEI+G + DYCDEENTQYPCNPSKGY
Sbjct: 123 DSKREIAAFFAHVTHETG-----------HFCFIEEIDGATRDYCDEENTQYPCNPSKGY 182

Query: 220 YGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFG 279
           +GRGPIQLSWNFNYGPAGE IGFDGLNNPEIVAT+PVVSFKTALWYWM+FVRPVINQGFG
Sbjct: 183 FGRGPIQLSWNFNYGPAGENIGFDGLNNPEIVATNPVVSFKTALWYWMNFVRPVINQGFG 242

Query: 280 ATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 323
           ATIRAINGALECDG N+ATVQRR+QFY QYCNQL V PG+NLTC
Sbjct: 243 ATIRAINGALECDGANTATVQRRVQFYNQYCNQLNVDPGNNLTC 275

[Close]

BLAST of MC03g0659 vs. TAIR 10
Match: AT3G54420.1 (homolog of carrot EP3-3 chitinase )

HSP 1 Score: 398.7 bits (1023), Expect = 4.6e-111
Identity = 183/260 (70.38%), Postives = 209/260 (80.38%), Query Frame = 0


Query: 64  AQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSPSVNDVVVADFVTEEFFNGI 123
           AQNCGC++ LCCS+FGFCG   D+CG GC++GPC  P   P  N V VA+ VT+EFFNGI
Sbjct: 28  AQNCGCSSELCCSQFGFCGNTSDYCGVGCQQGPCFAP---PPANGVSVAEIVTQEFFNGI 87

Query: 124 INQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGHVTHETGLILLYF 183
           I+QA + CAG  FYSR  FL+AL S+ RFGR+GS DDS+REIAAFF HVTHETG      
Sbjct: 88  ISQAASSCAGNRFYSRGAFLEALDSYSRFGRVGSTDDSRREIAAFFAHVTHETG------ 147

Query: 184 FCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFD 243
                 FC+IEEI+G S DYCDE  TQYPCNP+KGYYGRGPIQLSWNFNYGPAG AIGFD
Sbjct: 148 -----HFCYIEEIDGASKDYCDENATQYPCNPNKGYYGRGPIQLSWNFNYGPAGTAIGFD 207

Query: 244 GLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRAINGALECDGGNSATVQRRI 303
           GLN PE VATDPV+SFKTALWYW + V+PVI+QGFGATIRAINGALECDG N+ATVQ R+
Sbjct: 208 GLNAPETVATDPVISFKTALWYWTNRVQPVISQGFGATIRAINGALECDGANTATVQARV 267

Query: 304 QFYTQYCNQLGVAPGDNLTC 324
           ++YT YC QLGV PG+NLTC
Sbjct: 268 RYYTDYCRQLGVDPGNNLTC 273

[Close]

BLAST of MC03g0659 vs. TAIR 10
Match: AT2G43590.1 (Chitinase family protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.1e-83
Identity = 150/284 (52.82%), Postives = 187/284 (65.85%), Query Frame = 0


Query: 42  AAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPC--NI 101
           A  K SL+L   L+        +QNCGCA  LCCS+FG+CGT + +CG GCR GPC  + 
Sbjct: 2   AFTKISLVLLLCLLGFFSETVKSQNCGCAPNLCCSQFGYCGTDDAYCGVGCRSGPCRGSG 61

Query: 102 PPLSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVD 161
            P   SV  +     VT+ FFN IINQA  GCAG+ FY+R +F+ A  +F  F       
Sbjct: 62  TPTGGSVGSI-----VTQGFFNNIINQAGNGCAGKRFYTRDSFVNAANTFPNFAN----S 121

Query: 162 DSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGY 221
            ++REIA  F H THETG            FC+IEEING + +YC   NTQYPC P KGY
Sbjct: 122 VTRREIATMFAHFTHETG-----------HFCYIEEINGATRNYCQSSNTQYPCAPGKGY 181

Query: 222 YGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFG 281
           +GRGPIQLSWN+NYG  G+++G D L  PE+V ++P V+F+T LW+WM+ VRPV+NQGFG
Sbjct: 182 FGRGPIQLSWNYNYGACGQSLGLDLLRQPELVGSNPTVAFRTGLWFWMNSVRPVLNQGFG 241

Query: 282 ATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           ATIRAING +EC+GGNS  V  RI +Y  YC QLGV PG NL+C
Sbjct: 242 ATIRAING-MECNGGNSGAVNARIGYYRDYCGQLGVDPGPNLSC 264

[Close]

BLAST of MC03g0659 vs. TAIR 10
Match: AT2G43580.1 (Chitinase family protein )

HSP 1 Score: 290.4 bits (742), Expect = 1.7e-78
Identity = 142/282 (50.35%), Postives = 183/282 (64.89%), Query Frame = 0


Query: 42  AAAKRSLLLAAALIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPP 101
           A  K  L+L  +L+        +QNC CA  LCCS+FG+CGT  D+CGS C+ GPC +  
Sbjct: 2   ALTKIFLILLLSLLGLYSETVKSQNCDCAPNLCCSQFGYCGTTADYCGSTCQSGPCRVG- 61

Query: 102 LSPSVNDVVVADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDS 161
             P     +V + VT+ FFN IINQA  GCAG+ FY+R +F+ A  +F  F        +
Sbjct: 62  -GPPTGAGLVGNIVTQIFFNNIINQAGNGCAGKSFYTRDSFINATNTFPSFAN----TVT 121

Query: 162 KREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEENTQYPCNPSKGYYG 221
           +REIA  F H T+ETG            FC+IEEING S   CD+ N QYPC P+K Y+G
Sbjct: 122 RREIATMFAHFTYETG-----------HFCYIEEINGASRVMCDQNNRQYPCAPAKSYHG 181

Query: 222 RGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGAT 281
           RGP+ LSWNFNYG  G+++G D L  PE+V+++PVV+F+TALW+WM  VRPV+NQGFGAT
Sbjct: 182 RGPLLLSWNFNYGACGQSLGLDLLRQPELVSSNPVVAFRTALWFWMKSVRPVLNQGFGAT 241

Query: 282 IRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           IRAI+G  +CDG N   V  RI +Y  YC QLG+ PG N+TC
Sbjct: 242 IRAISG-FDCDGRNLGGVNARIGYYRDYCGQLGLDPGANITC 265

[Close]

BLAST of MC03g0659 vs. TAIR 10
Match: AT2G43570.1 (chitinase, putative )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 139/277 (50.18%), Postives = 177/277 (63.90%), Query Frame = 0


Query: 54  LIIGVIGGAAAQNCGCAAGLCCSRFGFCGTGEDFCGSGCREGPCNIPPLSPSVNDVVVA- 113
           LI+ V    A+QNCGCA+  CCS++G+CGT ++FCG GC+ GPC     S    D  V+ 
Sbjct: 21  LILTVSKPVASQNCGCASDFCCSKYGYCGTTDEFCGEGCQAGPCR---SSGGGGDPAVSL 80

Query: 114 -DFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQSFDRFGRIGSVDDSKREIAAFFGH 173
              VT +FFN I+NQ    C G+GFY+  TF+ A  S+  FG       SKREIAAFF H
Sbjct: 81  EGTVTPDFFNSILNQR-GDCPGKGFYTHDTFMAAANSYPSFG----ASISKREIAAFFAH 140

Query: 174 VTHETGLILLYFFCWNIDFCFIEEINGP----SGDYCDEENTQYPCNPSKGYYGRGPIQL 233
           V  ETG +           C+IEEI+GP    SG+YCD E  ++PC   KGYYGRG IQL
Sbjct: 141 VAQETGFM-----------CYIEEIDGPAKAASGEYCDTEKPEFPCAQGKGYYGRGAIQL 200

Query: 234 SWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMSFVRPVINQGFGATIRAING 293
           SWN+NYG  G+A+  + L +PE VA D V++FKTA W+W + VR     GFGATIRA+N 
Sbjct: 201 SWNYNYGLCGKALDENLLASPEKVAQDQVLAFKTAFWFWTTNVRTSFKSGFGATIRAVN- 260

Query: 294 ALECDGGNS-ATVQRRIQFYTQYCNQLGVAPGDNLTC 324
           + EC GG+S A    RI+++  YC +LGVAPGDNLTC
Sbjct: 261 SRECSGGDSTAKAANRIKYFQDYCGKLGVAPGDNLTC 277

[Close]

BLAST of MC03g0659 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 248.4 bits (633), Expect = 7.6e-66
Identity = 128/295 (43.39%), Postives = 181/295 (61.36%), Query Frame = 0


Query: 42  AAAKRSLLLAAALIIGVIGGAAAQNC---GCAAGLCCSRFGFCGTGEDFCGSGCREGPCN 101
           A  K++L++    +  + G A +QNC   GC   +CCSR+G+CGT + +CG+GC+ GPCN
Sbjct: 6   AILKKALIIFLFTLTIMTGTAFSQNCGTNGCKGNMCCSRWGYCGTTKAYCGTGCQSGPCN 65

Query: 102 IPPL---SPSVNDVV-------VADFVTEEFFNGIINQADAGCAGRGFYSRATFLQALQS 161
             P    +PS +  +       +A  +T  FFN I+++  +GC  +GFY+R  F+ A +S
Sbjct: 66  SKPKPTPTPSGSGGLNAGPRGTIASVITPAFFNSIMSKVGSGCPAKGFYTRQAFIAAAES 125

Query: 162 FDRFGRIGSVDDSKREIAAFFGHVTHETGLILLYFFCWNIDFCFIEEINGPSGDYCDEEN 221
           F  +   G+V  +KREIAA     +HE+G            FC+ EEI    G YC   +
Sbjct: 126 FAAYK--GTV--AKREIAAMLAQFSHESG-----------SFCYKEEI--ARGRYC-SPS 185

Query: 222 TQYPCNPSKGYYGRGPIQLSWNFNYGPAGEAIGFDGLNNPEIVATDPVVSFKTALWYWMS 281
           T YPC P K YYGRGPIQ++WN+NYG AG+ +G   L +P++VA  P V+F+ A+W+W  
Sbjct: 186 TTYPCQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFWNK 245

Query: 282 FVRPVINQGFGATIRAINGALECDGGNSATVQRRIQFYTQYCNQLGVAPGDNLTC 324
            VRPV++QGFGAT R ING  EC+GG  A VQ R+  Y  +C +LGV PG NL+C
Sbjct: 246 NVRPVLSQGFGATTRRINGG-ECNGGRPAAVQSRVNHYLDFCKKLGVTPGTNLSC 281

The following BLAST results are available for this feature:

BLAST of MC03g0659 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U5	6.4e-110	70.38	Endochitinase EP3 OS=Arabidopsis thaliana OX=3702 GN=EP3 PE=1 SV=1	[more]
P27054	1.4e-104	66.06	Endochitinase PR4 OS=Phaseolus vulgaris OX=3885 GN=CHI4 PE=2 SV=1	[more]
Q6K8R2	2.9e-94	57.54	Chitinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht6 PE=2 SV=1	[more]
Q7Y1Z0	4.3e-90	55.40	Chitinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht5 PE=2 SV=1	[more]
O04138	3.1e-88	54.36	Chitinase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht4 PE=2 SV=2	[more]

BLAST of MC03g0659 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140102.1	1.27e-201	95.79	endochitinase EP3-like [Momordica charantia]	[more]
XP_023001456.1	3.80e-181	86.38	endochitinase EP3 [Cucurbita maxima]	[more]
XP_038894874.1	4.92e-181	84.86	endochitinase EP3-like [Benincasa hispida]	[more]
XP_023520063.1	2.20e-180	86.02	endochitinase EP3 [Cucurbita pepo subsp. pepo]	[more]
KAG7019501.1	4.43e-180	86.02	Endochitinase EP3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC03g0659 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CFV8	6.16e-202	95.79	endochitinase EP3-like OS=Momordica charantia OX=3673 GN=LOC111010839 PE=4 SV=1	[more]
A0A6J1KIN5	1.84e-181	86.38	endochitinase EP3 OS=Cucurbita maxima OX=3661 GN=LOC111495584 PE=4 SV=1	[more]
A0A6J1EGZ6	6.15e-180	85.66	endochitinase EP3 OS=Cucurbita moschata OX=3662 GN=LOC111434008 PE=4 SV=1	[more]
A0A5A7UFI0	1.08e-177	83.45	Endochitinase EP3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4...	[more]
A0A1S3BAS2	1.08e-177	83.45	endochitinase EP3-like OS=Cucumis melo OX=3656 GN=LOC103487878 PE=4 SV=1	[more]

BLAST of MC03g0659 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54420.1	4.6e-111	70.38	homolog of carrot EP3-3 chitinase	[more]
AT2G43590.1	3.1e-83	52.82	Chitinase family protein	[more]
AT2G43580.1	1.7e-78	50.35	Chitinase family protein	[more]
AT2G43570.1	1.2e-71	50.18	chitinase, putative	[more]
AT2G43610.1	7.6e-66	43.39	Chitinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 66..97 e-value: 3.8E-9 score: 46.4
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 61..97 e-value: 1.4E-6 score: 28.6
IPR001002	Chitin-binding, type 1	PROSITE	PS50941	CHIT_BIND_I_2	coord: 64..99 score: 9.344389
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 115..268 e-value: 1.7E-46 score: 158.9 coord: 276..323 e-value: 5.4E-11 score: 42.7
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 131..153
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00774	CHITINASE_19_2	coord: 257..267
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 194..251 e-value: 9.4E-27 score: 94.6
None	No IPR available	GENE3D	1.10.530.10		coord: 110..323 e-value: 6.4E-57 score: 193.8
None	No IPR available	PIRSR	PIRSR001060-2	PIRSR001060-2	coord: 45..323 e-value: 2.3E-106 score: 353.7
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 48..323
None	No IPR available	PANTHER	PTHR22595:SF131	ENDOCHITINASE EP3	coord: 48..323
None	No IPR available	CDD	cd00035	ChtBD1	coord: 61..92 e-value: 4.35539E-7 score: 43.9134
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 115..323 e-value: 5.21013E-90 score: 266.603
IPR036861	Endochitinase-like superfamily	GENE3D	3.30.60.10		coord: 60..106 e-value: 1.2E-9 score: 40.1
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 61..101
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 37..323 e-value: 3.0E-102 score: 340.0
IPR018371	Chitin-binding, type 1, conserved site	PROSITE	PS00026	CHIT_BIND_I_1	coord: 69..88
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 111..323

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0659.1	MC03g0659.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
biological_process	GO:0006952	defense response
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding