MC03g0553 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0553
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA-directed RNA polymerases II and IV subunit 5A
Location	MC03: 12298772 .. 12301626 (+)
RNA-Seq Expression	MC03g0553
Synteny	MC03g0553

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTTTATTTTTTGCCCTAATCAAGATTGCATCTTTTAAAAGAGAACAGTGAGCGCGAGCATGCGCGCGCCAAAACCCAGCCGCGGCGGAGGGAAGACGAAAACGAAAATATTTTCGAGAGAGGCTCCGAATCCAAAATTTCTTTCTTCCGTCGAATCAATTCAATGAGATCTTAATCAGGTTATTTCGACACTTTCTTTTGTTCTTCTTAGAATTACTCTTGTTTTTCGTTCTCTGATTCTATCGTTATTTCCTTGATCGGTGTGATTTTGTTCTTATTGTTGCCTGGATTCAAGTCTAGGGTGGATTATTCCCCTTCTCGCAAGGGTAAATCAATACCCTCTTGAATAATATATACGCAAATCACAAATCTATACTGTTTCGGAAATTGATGTGTCTCAATTTCACGTCTATGTTGAGTGTCCGTGATTTGGTGTTTCCCATTTATTTGGCTTTGAAATTCCCATATGCTACAGGATCAATTGAGTCGATTGAAATGTGGATGAATTTTATCTTTCCTTCTATTTTTTTCGTTTTCCATTTTCTTTTCCCTGTTTCTGGTTTTGGAATTGAGTTTTTATATTGTTCTATGTGCTTCTTGCTTTGAATGATGACCGAGTGGTGACTTATTTGAACATGTATCAGCTGAAAGAGATGGTGTTAGCAGACGAGGAAATCACTAGACTCTTTAGAATACGAAAAACTGTGTTGCAGATGTTAAAAGACCGAGGCTACTTTGTTGGAGATTTCGAGATTGGCATGTCTAGAGAGCAATTTAGGAATAAGTATGGTGAGAGCATGAAAAGAGAAGACCTTGTTATCAATAAAGCCAAGAGAAACGATAGCTCTGACCAGGTATAATCTCATGCTATGCAATAATAATATGAGATTTTAGTGACTCATGCAGTACATTACCACAGGAAAAATTTCTATTTGAGTTCTTCTCAAGTACTTTGACATGGAAAATACTTCACTATTGATCTTTGATTAGTATAATCATATTATTTGATAAATATTTCCCATGTTTATGCAGAGTTTAGTTATAGGAATGTACAAGCTATTTGTTAATTCTTTTATTTGCGACTCTTTTATACAGATATATATTTTCTTTCCTGAAGAGCAGAAGGTTGGTGTCAAGACAATGAAAACCTATACAAATCGTATGAAATCAGAGAACGTTTTTAGAGCAATATTGGTTGTTCAACAGAATTTGACCCCCTTTGCTCGTACTTGCATAGGTGAAATATCTTCTAAATTCCATTTGGAGGTTTTCCAGGTAAATGTTAAATTTCTAACTGTTTCCTGGAGTTTTCTAATGTTGGATTGAATAAAATAGCATCATCATATGCCAGAAATTTAATGAGAACCGGAGGGACCTCGTATGCTTCTTCCTCAAAACTAGTTATCATTGAAAGATTGTTACGTTGAATTCCATCTAGAATTTCAATTGAAGTTAAGAGATCTAAGCAATTACTCGTTTAATCCCTCATGTATAGCTTTTTTTTTTGGTTTTATAAGAAATCTGAAACATATGTATTATATAGACAAAAGAGCACTAAGAACTGGGTAGAGGGCACCCCGCCCAAGAGCAACTAGAAGAGAGCTTTTAAATGGCTGACAATCATGCTAAGGCTTAGTTACAAAAGTTTTTTTCTATAATTAAGCATACACCAAGAAGATACCACCAAGAAGAGGTGTACTAAACATTCCTATAAAAAATATCAAAAGTGTAGGCTTTATCTTTGACGATTTCTTTCCAACTTAATGTGTCAAAGAAGAGCTCTAGCCACACAATTCCAATTCCAAAGGACCTTTTCTTTTCAAGGAAACATCCAACCTGCAGTCCTTTATACAGCCAACAGTCTATTTGCTTGGCAAAACAAACCGAGATGCCAAAAATATCCAATAGATAATGCCAACCTCGAACCACATAAGGACAGTGGATAGATAAATGATAAATGGACTCGCTACTTTTGAGGCATAAACTGCACACCAAGGGAGAAAGAGCTCAGTTTCTACATTTCCCTGCTTTAGCTACAAATTTCTTGTGATTTCCATAATACCGTGCCTACTCTTAATTTACTGAAGTTAATGCCTACCCATTTGTCACTTGTTGATGCAGGAAGCAGAATTGATGGTCAATATTAAAGATCATGTTCTCATTCCCGAGCACCAGTTGCTGACAACTGAAGAGAAGAAGACGCTGCTGGAGAGGTATACTGTAAAAGAGACGCAGGTTAGTATCAAATTCTTTTCCCCGAAAGACACCACATGTTGAGAATTAATTTCTAAACACATACTGAAACGTGTATCTTTTAGTTTTTAATGTGATTTTGAATTTTGCTTCAGCTACCTCGCATTCAGGTCACTGATCCAATTGCTAGATATTACGGGCTGAAGCGTGGACAAGTTGTGAAGATTATCCGACCAAGTGAGACGGCAGGAAGATATGTGACATATAGATATGTTGTTTGATATTGACCTGATTGGCCATTGAAGTTACGGTTCTTGGAGCAGTGGTTTGAATTTTAGGCAATGTTTCTGTTAGCGTGGGCTCAATCTTGTGGGGAGTTTCTGTAGGATTTTGATAGAATTTCAGCGGTAGTGGACTGTTATTCATACACTAAACTGAGTAGGATAATTACATTTGTTTCTTTTCGAGTTTGACAAGTGATGATGAAGATTCAAATTTTCAACCTTTTGGTAAAAAATACGTCTTAACTAATTAACCTATGTTCAGGCTGACGAAGATATGTTTCTCTTCGATTTAACCTTCTGTTGTAAGATAGATTCTTATGTGCACCTGTAGTTCATATAATATTTGCCAGTTCTAAACTTCGATAGAACTTTTGAT

mRNA sequence

ATTTTTTATTTTTTGCCCTAATCAAGATTGCATCTTTTAAAAGAGAACAGTGAGCGCGAGCATGCGCGCGCCAAAACCCAGCCGCGGCGGAGGGAAGACGAAAACGAAAATATTTTCGAGAGAGGCTCCGAATCCAAAATTTCTTTCTTCCGTCGAATCAATTCAATGAGATCTTAATCAGCTGAAAGAGATGGTGTTAGCAGACGAGGAAATCACTAGACTCTTTAGAATACGAAAAACTGTGTTGCAGATGTTAAAAGACCGAGGCTACTTTGTTGGAGATTTCGAGATTGGCATGTCTAGAGAGCAATTTAGGAATAAGTATGGTGAGAGCATGAAAAGAGAAGACCTTGTTATCAATAAAGCCAAGAGAAACGATAGCTCTGACCAGATATATATTTTCTTTCCTGAAGAGCAGAAGGTTGGTGTCAAGACAATGAAAACCTATACAAATCGTATGAAATCAGAGAACGTTTTTAGAGCAATATTGGTTGTTCAACAGAATTTGACCCCCTTTGCTCGTACTTGCATAGGTGAAATATCTTCTAAATTCCATTTGGAGGTTTTCCAGGAAGCAGAATTGATGGTCAATATTAAAGATCATGTTCTCATTCCCGAGCACCAGTTGCTGACAACTGAAGAGAAGAAGACGCTGCTGGAGAGGTATACTGTAAAAGAGACGCAGCTACCTCGCATTCAGGTCACTGATCCAATTGCTAGATATTACGGGCTGAAGCGTGGACAAGTTGTGAAGATTATCCGACCAAGTGAGACGGCAGGAAGATATGTGACATATAGATATGTTGTTTGATATTGACCTGATTGGCCATTGAAGTTACGGTTCTTGGAGCAGTGGTTTGAATTTTAGGCAATGTTTCTGTTAGCGTGGGCTCAATCTTGTGGGGAGTTTCTGTAGGATTTTGATAGAATTTCAGCGGTAGTGGACTGTTATTCATACACTAAACTGAGTAGGATAATTACATTTGTTTCTTTTCGAGTTTGACAAGTGATGATGAAGATTCAAATTTTCAACCTTTTGGTAAAAAATACGTCTTAACTAATTAACCTATGTTCAGGCTGACGAAGATATGTTTCTCTTCGATTTAACCTTCTGTTGTAAGATAGATTCTTATGTGCACCTGTAGTTCATATAATATTTGCCAGTTCTAAACTTCGATAGAACTTTTGAT

Coding sequence (CDS)

ATGGTGTTAGCAGACGAGGAAATCACTAGACTCTTTAGAATACGAAAAACTGTGTTGCAGATGTTAAAAGACCGAGGCTACTTTGTTGGAGATTTCGAGATTGGCATGTCTAGAGAGCAATTTAGGAATAAGTATGGTGAGAGCATGAAAAGAGAAGACCTTGTTATCAATAAAGCCAAGAGAAACGATAGCTCTGACCAGATATATATTTTCTTTCCTGAAGAGCAGAAGGTTGGTGTCAAGACAATGAAAACCTATACAAATCGTATGAAATCAGAGAACGTTTTTAGAGCAATATTGGTTGTTCAACAGAATTTGACCCCCTTTGCTCGTACTTGCATAGGTGAAATATCTTCTAAATTCCATTTGGAGGTTTTCCAGGAAGCAGAATTGATGGTCAATATTAAAGATCATGTTCTCATTCCCGAGCACCAGTTGCTGACAACTGAAGAGAAGAAGACGCTGCTGGAGAGGTATACTGTAAAAGAGACGCAGCTACCTCGCATTCAGGTCACTGATCCAATTGCTAGATATTACGGGCTGAAGCGTGGACAAGTTGTGAAGATTATCCGACCAAGTGAGACGGCAGGAAGATATGTGACATATAGATATGTTGTTTGA

Protein sequence

MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKRNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSKFHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGLKRGQVVKIIRPSETAGRYVTYRYVV

Homology

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BLAST of MC03g0553 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 5.9e-93
Identity = 168/205 (81.95%), Postives = 193/205 (94.15%), Query Frame = 0


Query: 2   VLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKR 61
           +L +EE+ RL+RI+KT++QML+DRGYF+ D E+ M+++QF  K+G++MKREDLV  KAKR
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSKF 121
           ND+SDQ+YIFFP+E KVGVKTMK YTNRMKSENVFRAILVVQQNLTPFARTCI EISSKF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 HLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGL 181
           HLEVFQEAE++VNIK+HVL+PEHQ+LTTEEKKTLLERYTVKETQLPRIQVTDPIARY+GL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGL 180

Query: 182 KRGQVVKIIRPSETAGRYVTYRYVV 207
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 KRGQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of MC03g0553 vs. ExPASy Swiss-Prot
Match: Q9N5K2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis elegans OX=6239 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.4e-57
Identity = 115/211 (54.50%), Postives = 155/211 (73.46%), Query Frame = 0


Query: 3   LADEEIT--RLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGE-----SMKREDLV 62
           +AD+E+   RL+RIRKTVLQM+ DRGY V   E+    E F+ +YG+        R DL 
Sbjct: 1   MADDELETYRLWRIRKTVLQMVHDRGYLVAQDELDQPLETFKVQYGDRPSEKKPARSDLT 60

Query: 63  INKAKRNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIG 122
           I  A  +D +DQ+++FFPE+ K+G+KT+K    +M+ +N+ RAI+VVQ  +TP A+  IG
Sbjct: 61  ILVAHNDDPADQMFVFFPEDAKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIG 120

Query: 123 EISSKFHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPI 182
           +++ K+ LE F EAELMVNI +H L+PEH ++T EEK  LL RY +K++QLPRIQ  DP+
Sbjct: 121 DMAPKYMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPV 180

Query: 183 ARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 207
           ARY+GL+RGQVVKIIRPSETAGRY+TYR VV
Sbjct: 181 ARYFGLRRGQVVKIIRPSETAGRYITYRLVV 211

[Close]

BLAST of MC03g0553 vs. ExPASy Swiss-Prot
Match: A8XGH1 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis briggsae OX=6238 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.3e-57
Identity = 114/211 (54.03%), Postives = 154/211 (72.99%), Query Frame = 0


Query: 3   LADEEIT--RLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGE-----SMKREDLV 62
           +AD+E+   RL+RIRKTVLQM+ DRGY V   E+    E F+  +G+        R DL 
Sbjct: 1   MADDELETYRLWRIRKTVLQMVHDRGYLVAQEELDQPLETFKETFGDRPSEKKPARSDLT 60

Query: 63  INKAKRNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIG 122
           I  A  +D +DQ+++FFPE+ K+G+KT+K    +M+ +N+ RAI+VVQ  +TP A+  IG
Sbjct: 61  ILVAHNDDPADQMFVFFPEDTKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIG 120

Query: 123 EISSKFHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPI 182
           +++ K+ LE F EAELMVNI +H L+PEH ++T EEK  LL RY +K++QLPRIQ  DP+
Sbjct: 121 DMAPKYMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPV 180

Query: 183 ARYYGLKRGQVVKIIRPSETAGRYVTYRYVV 207
           ARY+GL+RGQVVKIIRPSETAGRY+TYR VV
Sbjct: 181 ARYFGLRRGQVVKIIRPSETAGRYITYRLVV 211

[Close]

BLAST of MC03g0553 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 9.9e-56
Identity = 106/206 (51.46%), Postives = 151/206 (73.30%), Query Frame = 0


Query: 5   DEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGES-----MKREDLVINKA 64
           +EE  RL++IRKT++Q+  DRGY V   E+  + E+FR ++G+       +R DL +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISS 124
             +D +DQ+++FFPEE KVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYY 184
           K+ LE F + EL++NI +H L+PEH ++T EE   LL RY ++E QLPRIQ  DP+ARY+
Sbjct: 124 KYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVARYF 183

Query: 185 GLKRGQVVKIIRPSETAGRYVTYRYV 206
           G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 GIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of MC03g0553 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.7e-55
Identity = 105/206 (50.97%), Postives = 152/206 (73.79%), Query Frame = 0


Query: 5   DEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGES-----MKREDLVINKA 64
           +EE  RL++IRKT++Q+  DRGY V   E+  + E+F+ ++G+       +R DL +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISS 124
             +D +DQ+++FFPEE KVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYY 184
           K+ LE F + EL++NI +H L+PEH ++T EE   LL RY ++E+QLPRIQ  DP+ARY+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYF 183

Query: 185 GLKRGQVVKIIRPSETAGRYVTYRYV 206
           G+KRGQVVKIIRPSETAGRY+TYR V
Sbjct: 184 GIKRGQVVKIIRPSETAGRYITYRLV 209

[Close]

BLAST of MC03g0553 vs. NCBI nr
Match: XP_022142704.1 (DNA-directed RNA polymerases II and IV subunit 5A [Momordica charantia] >XP_022142705.1 DNA-directed RNA polymerases II and IV subunit 5A [Momordica charantia])

HSP 1 Score: 406 bits (1043), Expect = 1.06e-142
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK
Sbjct: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK
Sbjct: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG
Sbjct: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. NCBI nr
Match: XP_022991826.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023547519.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389 bits (998), Expect = 7.67e-136
Identity = 192/206 (93.20%), Postives = 203/206 (98.54%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVL++EEITRLFRIRKTVLQMLKDRGYFVGDFEI MSREQF++KYGESMKRED+VINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIY+FFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCI EISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VN+KDHVL+PEHQLLT EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. NCBI nr
Match: XP_022953770.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata])

HSP 1 Score: 388 bits (997), Expect = 1.09e-135
Identity = 192/206 (93.20%), Postives = 203/206 (98.54%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVL++EEITRLFRIRKTVLQMLKDRGYFVGDFEI MSREQF++KYGESMKRED+VINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDVVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIY+FFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCI EISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VN+KDHVL+PEHQLLT EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. NCBI nr
Match: XP_022959553.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959554.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959555.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_023004795.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004796.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004797.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023513561.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo] >XP_023513563.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 388 bits (996), Expect = 1.55e-135
Identity = 192/206 (93.20%), Postives = 202/206 (98.06%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVLA+EEITRLFR+RKTVLQMLKDRGYFVGDFE+ MSREQF+NKYGESMKRED+VINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIYIFFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCI EIS+K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VNIKDHVL+PEHQLL+ EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. NCBI nr
Match: XP_022143609.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia])

HSP 1 Score: 385 bits (990), Expect = 1.27e-134
Identity = 191/206 (92.72%), Postives = 202/206 (98.06%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVL++EEITRLFRIRKTVLQMLKDR YFVGDFEI MSREQF++KYGE+MKRED+VINKA+
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKAR 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIY+FFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCI EISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VNIKDHVL+PEHQLLT EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. ExPASy TrEMBL
Match: A0A6J1CLP6 (DNA-directed RNA polymerases II and IV subunit 5A OS=Momordica charantia OX=3673 GN=LOC111012752 PE=3 SV=1)

HSP 1 Score: 406 bits (1043), Expect = 5.12e-143
Identity = 206/206 (100.00%), Postives = 206/206 (100.00%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK
Sbjct: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK
Sbjct: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG
Sbjct: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. ExPASy TrEMBL
Match: A0A6J1JRV4 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111488357 PE=3 SV=1)

HSP 1 Score: 389 bits (998), Expect = 3.72e-136
Identity = 192/206 (93.20%), Postives = 203/206 (98.54%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVL++EEITRLFRIRKTVLQMLKDRGYFVGDFEI MSREQF++KYGESMKRED+VINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIY+FFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCI EISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VN+KDHVL+PEHQLLT EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. ExPASy TrEMBL
Match: A0A6J1GQK9 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111456196 PE=3 SV=1)

HSP 1 Score: 388 bits (997), Expect = 5.28e-136
Identity = 192/206 (93.20%), Postives = 203/206 (98.54%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVL++EEITRLFRIRKTVLQMLKDRGYFVGDFEI MSREQF++KYGESMKRED+VINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDVVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIY+FFPEEQKVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCI EISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VN+KDHVL+PEHQLLT EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. ExPASy TrEMBL
Match: A0A6J1KT45 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111497988 PE=3 SV=1)

HSP 1 Score: 388 bits (996), Expect = 7.50e-136
Identity = 192/206 (93.20%), Postives = 202/206 (98.06%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVLA+EEITRLFR+RKTVLQMLKDRGYFVGDFE+ MSREQF+NKYGESMKRED+VINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIYIFFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCI EIS+K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VNIKDHVL+PEHQLL+ EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. ExPASy TrEMBL
Match: A0A6J1H699 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111460591 PE=3 SV=1)

HSP 1 Score: 388 bits (996), Expect = 7.50e-136
Identity = 192/206 (93.20%), Postives = 202/206 (98.06%), Query Frame = 0


Query: 1   MVLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAK 60
           MVLA+EEITRLFR+RKTVLQMLKDRGYFVGDFE+ MSREQF+NKYGESMKRED+VINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 120
           RNDSSDQIYIFFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCI EIS+K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 180
           FHLEVFQEAEL+VNIKDHVL+PEHQLL+ EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

Query: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 LKRGQVVKIIRPSETAGRYVTYRYVV 206

[Close]

BLAST of MC03g0553 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 341.7 bits (875), Expect = 4.2e-94
Identity = 168/205 (81.95%), Postives = 193/205 (94.15%), Query Frame = 0


Query: 2   VLADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKR 61
           +L +EE+ RL+RI+KT++QML+DRGYF+ D E+ M+++QF  K+G++MKREDLV  KAKR
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NDSSDQIYIFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSKF 121
           ND+SDQ+YIFFP+E KVGVKTMK YTNRMKSENVFRAILVVQQNLTPFARTCI EISSKF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 HLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGL 181
           HLEVFQEAE++VNIK+HVL+PEHQ+LTTEEKKTLLERYTVKETQLPRIQVTDPIARY+GL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGL 180

Query: 182 KRGQVVKIIRPSETAGRYVTYRYVV 207
           KRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 181 KRGQVVKIIRPSETAGRYVTYRYVV 205

[Close]

BLAST of MC03g0553 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 181.8 bits (460), Expect = 5.6e-46
Identity = 95/206 (46.12%), Postives = 142/206 (68.93%), Query Frame = 0


Query: 5   DEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESM---KREDLVINKAKR 64
           D+EITR+F++R+TVLQML+DRGY + + ++ + RE+F  ++ ++M    +E L ++  K 
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 65  NDSSDQIYIFFPEEQKVGVKTM-KTYTNRMKSENVFRAILVVQQNLTPFARTCIGEISSK 124
            + +D+IY+F+PE  KVGV  + K    +M+ + V R I+VV   +T  AR  + E++  
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKM 124

Query: 125 FHLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 184
             +EVF+EAEL+ NI +H L+ ++ +L  + KK LL  YTV++TQLPRI VTDP+ARYYG
Sbjct: 125 LTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYYG 184

Query: 185 LKRGQVVKIIRPSETAGRYVTYRYVV 207
           LKRGQVVKI R   T+  Y TYR+ V
Sbjct: 185 LKRGQVVKIRRSDATSLDYYTYRFAV 210

[Close]

BLAST of MC03g0553 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 134.8 bits (338), Expect = 7.8e-32
Identity = 79/200 (39.50%), Postives = 123/200 (61.50%), Query Frame = 0


Query: 7   EITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKRNDSSD 66
           E  + +  R+T ++ML+DRGY V D +I +S +QFR  YGE    + L I+   R DSS 
Sbjct: 20  ECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFDSSK 79

Query: 67  QIYIFFPEEQKVGVKTMKTY-TNRMKSENVFRAILVVQQNLTPFARTCIGEISSKFHLEV 126
           +I + F     V V  M+    + +  EN+   ILV+Q ++T  A   +   S  F +E+
Sbjct: 80  KISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFS--FKVEL 139

Query: 127 FQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGLKRGQ 186
           F+  +L+VN+  HVL P+HQ+L  +EK++LL++++++E QLPR+   DPI RYYGL+ GQ
Sbjct: 140 FEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGLETGQ 199

Query: 187 VVKIIRPSETAGRYVTYRYV 206
           V+K+    E +  +VTYR V
Sbjct: 200 VMKVTYKDELSESHVTYRCV 217

[Close]

BLAST of MC03g0553 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 133.3 bits (334), Expect = 2.3e-31
Identity = 82/204 (40.20%), Postives = 120/204 (58.82%), Query Frame = 0


Query: 3   LADEEITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKRN 62
           L+ EE  R +  R+  LQML+DRGY V D +I +S   FR  YGE    + L I+   R+
Sbjct: 20  LSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRS 79

Query: 63  DSSDQIYIFFPEEQKVGVKTMKT-YTNRMKSENVFRAILVVQQNLTPFARTCIGEISSKF 122
           DS+ ++ I F     V V  +++   + +  E +   ILV+Q ++T  A   I   S  F
Sbjct: 80  DSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAIELFS--F 139

Query: 123 HLEVFQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGL 182
            +E+FQ  +L+VNI  H L P+HQ+L  EEK TLL++++++E QLPRI   D I RYYGL
Sbjct: 140 KVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIVRYYGL 199

Query: 183 KRGQVVKIIRPSETAGRYVTYRYV 206
           ++GQVVK+    E    +V +R V
Sbjct: 200 EKGQVVKVNYRGELTESHVAFRCV 221

[Close]

BLAST of MC03g0553 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 115.9 bits (289), Expect = 3.8e-26
Identity = 68/201 (33.83%), Postives = 114/201 (56.72%), Query Frame = 0


Query: 7   EITRLFRIRKTVLQMLKDRGYFVGDFEIGMSREQFRNKYGESMKREDLVINKAKRNDSSD 66
           E  R +  R T  +ML+DRGY V + E+ ++  +FR+ +GE  + E L I    R+D   
Sbjct: 35  ESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKK 94

Query: 67  QIYIFFPEEQKVGVKTMKTYTNRMKSE-NVFRAILVVQQNLTPFARTCIGEISSKFHLEV 126
           +I + F   + + VK+++    ++ +   +   ILV+Q  +  FA+  +   +  F +E 
Sbjct: 95  KILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKAL--TTFPFTVET 154

Query: 127 FQEAELMVNIKDHVLIPEHQLLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYGLKRGQ 186
           F   +L+VNI  H+  P+ ++L  EEK+ LL ++ +++ QLP +Q  D   RYYGLK+ Q
Sbjct: 155 FPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFVRYYGLKKKQ 214

Query: 187 VVKIIRPSETAGRYVTYRYVV 207
           VVKI    E  G +VTYR ++
Sbjct: 215 VVKITYSKEPVGDFVTYRCII 233

The following BLAST results are available for this feature:

BLAST of MC03g0553 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	5.9e-93	81.95	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q9N5K2	2.4e-57	54.50	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis ele...	[more]
A8XGH1	5.3e-57	54.03	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis bri...	[more]
Q5R587	9.9e-56	51.46	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
Q80UW8	1.7e-55	50.97	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]

BLAST of MC03g0553 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142704.1	1.06e-142	100.00	DNA-directed RNA polymerases II and IV subunit 5A [Momordica charantia] >XP_0221...	[more]
XP_022991826.1	7.67e-136	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_02...	[more]
XP_022953770.1	1.09e-135	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata]	[more]
XP_022959553.1	1.55e-135	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_...	[more]
XP_022143609.1	1.27e-134	92.72	DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia]	[more]

BLAST of MC03g0553 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLP6	5.12e-143	100.00	DNA-directed RNA polymerases II and IV subunit 5A OS=Momordica charantia OX=3673...	[more]
A0A6J1JRV4	3.72e-136	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1GQK9	5.28e-136	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KT45	7.50e-136	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H699	7.50e-136	93.20	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=...	[more]

BLAST of MC03g0553 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	4.2e-94	81.95	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	5.6e-46	46.12	RNA polymerase II fifth largest subunit, C	[more]
AT2G41340.1	7.8e-32	39.50	RNA polymerase II fifth largest subunit, D	[more]
AT3G57080.1	2.3e-31	40.20	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT3G54490.1	3.8e-26	33.83	RNA polymerase II fifth largest subunit, E	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 1..206 e-value: 5.2E-90 score: 298.4
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 3..130 e-value: 2.8E-47 score: 162.3
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 5..134
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 131..206 e-value: 3.8E-34 score: 118.2
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 131..206
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 133..205 e-value: 5.5E-34 score: 115.9
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 6..90 e-value: 2.7E-32 score: 111.2
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..206
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 1..206
IPR020609	Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit	HAMAP	MF_00025	RNApol_RpoH_RPB5	coord: 133..206 score: 22.614899

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0553.1	MC03g0553.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0055029	nuclear DNA-directed RNA polymerase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity