MC03g0533 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0533
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionsubtilisin-like protease SBT2.4
LocationMC03: 12104534 .. 12110826 (+)
RNA-Seq ExpressionMC03g0533
SyntenyMC03g0533
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGGATCATGGAGTTTTCTTCACTACTAATATTTCTACTGTTTTGGAGTTTTTTTCTTCCTTGTTTTACAGAAGAGAGAGAAATCTACTTGGTCTTAATGGAAGACGACGACCCAGTTGCCTTCCACCAAAGTGAGAGGACAACTAGTCCGAGCTTCGAGTTAAGTATCGTACAAGGACGGAGTGAAGAAGGAAGAAGAAAAACACTACTGCTTAGTTTTCTTTTTAATTCTAAAGCGGGTTTGATATGATGATTTTGTTTTTGATTTTTGAAAATAATAAGTCACGAATATGTTTTTAGAAAATGTGTTTCTATTTTTGAAACTCGACTAAAAATTCAAACGTGTTTTTTTAAGAAAGATGATAAATATAGGGAAAAGAAAACTTATTTTTTACCGTTTAATTAAACTTGGCATTAAAATCTCCGACCCTTTTAATTGAAGATATATGCATTGAATAGTTAAGCTATATACTCAAGCCGAAACATAGTTTTTAGATTCTTTCTCATCTGATCATAGTAGTATCTAATACTAGAATTATCGAGCACCCTCAAATAACTTCTCCCGATCGTCACTACAACTAAATATATATAAAAATCCTCTTAAAAAGTGTAAAAACTGACTGTTTCTTTTTGGTTTTTTTAAAAGAAGAAACAGCAAAGTTTCAAAAAAGGCAGCACCAAGAGAACTGGAGGGATTCCACGACCAGTTTCTTGAGAAAAGTCTAAGAGTGGAGAGCTACACGAAGCTTCACAGCTTCAAGCACATCATCAATGGGTTCGCGGTCCACACGACGCCATCGGAGGCGAGAAAGCTGAGAGAAGCTGAAGGGGTTGTGGCTGTGGAGAGAGACAGAGGAGTGAGGAAGATGACAACTTACACCCCTCAGTTTCTAGGGGTTCCCTCCACATTAAGGGCAACAGTGGGATGCAAGAGGAATGAAAGTAGTGGTGGAGAGGGGATTGTTATTGGGTTTGTGGACTCTGGGATTGACCCGACACACCCGAGTTTCGGTTTCGGTTTCGGTCTCGGTCTCGACGAGTCTTCTAAGTTTTGTGGGGTTTGTGAGGAAGGTCCTTTTTTTGGTTCAAATTCTTGCAATGGGAAGATTGTGGGAGCAAGATTTTTCTGTGCTGGGGCTCAGGCTGTTTCACAACTCAACTCTTCTGTGGATTTCCTCTCCCCTTTTGATGCTGAAGGCCATGGAAGGTAAGCCTTCAACCTTAGCTTTTGTGTTTATTTGTTTGTTTAACAGGGGTTCTTGTTTTGTAACGTTCTCGTTTCTTATTCATGTTTCTTCCTTTTTAGTAAGAAAAAATAGAGTTGCATGGCTTTCAACCGCTAAACACGCGTGGATTCAAAATAAATCAAACAAAATTTTTATTTAGGTTGGAATGAATTGGTAATCGAAACAATAAAATAAATAAGGAAACAAAACATTTTTGTATTGTTTCTCAAAACTTTTATAACATTTCGAGAATACTTCTAACAAATAATAAACGAGAAACAAAACGCTTAGATTTCTTAATAAGATTATAAATGAGAAACAAGAAACAATAATGTTATCAAATGGATCCTTAATTTTTAAACCTTTTATTTTGATGGTTAAAGTTTGTATAGAGGGCTACTTTTAAATTTTAAGTCTTTGTTTTTTATAATTATTTAGTTTTTTCTTTTTTAAAAATTATATATATATATATATATATTTTAAATTTGTCAATCTAAGTCTCTCAACTGATTAAAATATTTATCTCTGATTAAAAAGTTCGTAAGTTCGAATTTTCTCCTTCACACGTATTATACTCAAATATATATATATATATATATATATATATTTTGAAATTAATTTGGTATGCTAGTACGTCAAAGAATATTGGGTTTTAAAATATATGTGAGTTCATTGTAAATAATTGATGAGAAAAATTAAGTGAAAAAAATATAGAATATTTGAAAGTTTATAATATGAAGGAAAAAACCATTAAAAACTTAGGGTGGTGGGTTGACTTGGGACTTTAATTTGATTATAATCGTTATTATATTATATTTAACTGATCTTAATTTTCATTGAACTGATTGAAATCAAAACATATATTTTTCTATTTTAATTTAGTTGAATAAAAATATATTTGGACATGGTTATCAGCCACGTGGCATCTATAGCTGCTGGAAATGCAGGGGTTCCAGTCGTTGTTAATGGTTTTTTCTATGGATTGGCCAGTGGGATAGCGCCACGTGCACGGTACATTAACACATTCCCAACATATATGTCATTATTATACGAATTTTCAATTTTGGCTCATTAAATTTCAATTCCATTCGTATGCTTTTTAATATATAAAACTTCAATACTCTTTCTTACAAAATTTAGTGTTAAATACAGTAAAGAAAAAGTGATTTTTATTAAAAGATTTCATTACGTATAAAAATTCGTATTAAAATTTTACAAATAGGGATCAAAATAATCCCTTTTTAAAAATACATCACCAAAACAAACATTTTTTTTAGAGTATAATAGCCACAATAGTATTTAATCCATAAACATATATCATTGTGCTTAAGTTAGAATTAACTAATTTGGTTTAAAGTGATTTTTCTTTTTTCTGGACAGAATTGCTATTTATAAGGCTGTGTATCCAACAGTGGCTACTCTAACAGATGTAGTTGCAGCCATAGATCAAGTAAGTTCTTCATACGTCCATTTAATATCGAGTTTCGAAATAGATAGCTTAAGATTTGACGGGAATGCCAAAAACGAGTCAAAGTTTAAGAACCAAAATAGAATATTCGAAAGTTCGAGTTTCATGTCGGGATTTGTTTTGAACAAGTGCAGGCAATTATTGATGGAGTTGATATATTGGCATTGTCTGTTGGACCAAATGAACCACCAGAAGTAGGATTTACGTTTCTAAGCATATATGACATTGCTGTATTATCTGCCACACGAGCCGGAATCCTTGTGGTTCAGGCTGCCGGAAATAACGGCCCGGCCCCGTCAACCGTCGTCTCTTACAGCCCTTGGGCTGTCGGCGTGGCCGCTTCCGGCACCGACCGAGTTTATCCGGCCTCACTTCTCCTTGGCAATGGCCAAAACGTTGGAGGCACAGGCTTGTCTGGTATGTCTAATATGTTGATTAGAACTTGGTATAAATTAATTAAGAAGATACAAAATATGAAAGAATTAGATGTAATTCTCACTAACTGAAAGCAAAACTATGAGAAAAGTTTGTTTAATGTTAAAAAAAAAATGGAAATGTGGCTCATTGCTTGCTTTTATGCATATTAGTGCTATAACTTTCAGAGAAGTTCTCCCTTTTTACTTATCTAGTTCATCGTAATCATTTCTAGTTTGAGGAGCCCTGTTCAGAGATGAGATTCTCATGGCGAAAATTACCTATTTTGTAATTAATTTTAAATACTTTTAAATTTAAATTTTTAAATTTTTAAATATATATATATATAAATGAAAGAAAATTATATAATGAAAATTATAATGAGAAAATTTAGAGAGATGTGTTTTGTTAATGAAAAATAAATTAAAAAGATGAAAAAGCTATTGAGGAAAATTTTAAATAGAAATTTTATAGATTTATTTTTTAGATAACGTAAATTAGAAAGATAAAGAAAGTTATTAGAAGAAAAAAAAAAGATCAGAGAGAGAAAAGTTTGAGAAAAATGTTTCAATAAATTTTTTGGGAGGAAAACATTTTAGAGAGAAATTTGTTACTAAGAATTTGAAAATCGTTTTTGGAAAACATAAACGATTATCAAACAAATTTGTTTTTCAAAAACTAAAAACAGAAAACAAAATAATTATCAAACAACCTGTTTAACAAATCAAATTTAACTACTCTATTCATCTTCCTCAGGACCCACTCTGGGGAGTGAGTTTTTGCTACATAAGCTGGTATTGGCCAAGGATGCAGTGAACCAAAATGTGGCACTTCCCACTGAAGAGTGCCAATATCCTCAAGCTTTTGACCCAAATATTGTCCAAAACAGCATAGTAATCTGCTCCTTCTCTCAAGGATTCCTCAATGGAACCTCCACCCTCACAGCTATCCTTCACACTGCAGCAACACTCAGATTCATGGCTTTTGTTCTCGTTGCAAATCCGAGCTATGGCGACTTCATTGCAGAACCCATCCCCTTCAGAATTCCTGGCATTCTCGTCCCGAGCGTTTCAGATTCTCAGGTACAATATCTACTTTGATTCAAAACCAGTGAGAAACAAAATCGTTATCAAACAAGAATATCTGAGAATTTCTATTTGCGCTCGAAATAAATATAGGTGATATTGAAGTACTATGAGGAAAATATATGCAAGGATGGGAGGGGAATGGTTACAAACTTTAAAGGCAAAGCAGCCATAGGAGAAGGAAGATTTGCTTCATTTGGGGGCCAAGCTCCCACAGTTAGCAGATTTTCATCAAGAGGACCAGATTACATGAACAGTAATAGGACTGCAGCTGATGTGCTAAAGCCCGATATTCTCGCTCCAGGTCACCAAGTTTGGGCTGCTTGGAGCCCCCTTAGTGCCTCGGAGCCACTTTTAAAGGGTGAGTTGAGCCAGTGTAAAGTTCTGTTTCGGTCTCTAAACTTTTATTTATTTTCTGCTTAGTCACTTGATTTCGGTCCTTGAACTTTCATCACTTTTTGTTTTGGTCCTTTTTCGCTTTATGGATTGAATAGGATACCATTTTGCACTCTTGTCTGGAACAAGCATGGCAGCACCTCACATTGTGGGAATAGCAGCACTTATCAAGCAAAAGAATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCAATGTCCACAACTGCTACAAAGTATGATATGAATGGAGAGCTTATTCAGGCAGAGGGATATGGCATTCATAGTTTATATCCTTCTACTCCCTTTGATTTGGGCGCTGGCCTCGTCTCTCCGACCAATGCTTTAGACCCGGGATTGGTCTTCCCATCAGGTAAATTCCAACAATACACACTCTACCTTTTCACTATGTCGTATAATGTGGTTTTGAAAATCTAAAAGCACTTGAAAGGGAAAAAAAAACACTCAAACAATGTCCTTTTCCTCTAACAATGCTAGTTAGGAAAATGTTTTACTCGAAAATGCCATTCCTTGAATAGATTCGACACGCTCATAAAACAAGAAAACCATACCATGTCATTTCAACATGAAAATTGGATAGCTGAGATTATATTTGAGGATACATTATGATTCTAGTCACTATATTATGCCATTTTTCAGCATTTCGGTTCATGTTGTTACATAATTTGCATTTTTACTCTTACTATTCAGCATGATATACCACATCAATGGCTCCTTACTATTCAACCACATGATTAACATAAACTATAGTATATTTTAATAAAAGTACATGAACTAAAAATGAAACGAAGAAAAGCATAGATATCAAAACACTAAAAGTCGTTGAAGAAAACTGACCAGCCATCTTGAAACAGAATATGAAGACTATATAAACTTCTTGTGCTCTTTACCTGGCGTTGATCCTGCTGTCATCAAAAGCACCACTGGAAGACATTGCAATGCCTCTCTCTCACAGCCACACCCAGCAGATCTGAACCTTCCTTCACTCACAATATCATCACTGGTTGGGCAACAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGCAAAGTGGAGACATATGTGTGGTCGGTGATCCCGCCGAACGGGACAACAGTCGACATCAATCCGCCATGGTTCGCCATAGCGCCAGAAGGGGTCCAAAACTTGGAAATACAGATCAATGTCACACATAAAATGGATCACTTTAGCTTTGGGGAGATTGTTTTGACAGGAAGTTTAAACCATATTGCAAGGATCCCTTTGTCAGTTTTGGCTGTTTCTACATCCTAAAGGACAGGATTGGTTCATTTTCTTTGAAAGTCTCTCTATATTCCTTACTTTTAAAAGGGGGTAAAATTTTTCAAAAGATTATAATGTGTAAAATGTCAAATAAGTGATAGTTTCAAGATGAAACTAGTACACTAGTGTAGTTCTTAGAAATGAGCTCAAAAGTTTGAATATTACCAATATAGAAAGAAAGATCTTGTTGCTATAACATTTGAGAGCTAGTAGTTCTAATGGCAGACTGTTCTGTCTTTTTGTACAAAAAGGAAGTTTAGCCTGTCTTAAGAAAAAATCTTTTGAATGGTTTCTAAAGGTGAGATACAACTTTTCAATATCGATGGCGAATCTATATATAAATTCATGTGTAATTTCTGTTCTTTTAAATTTTAATTCATTTACAATTGTGGAACACAATTT

mRNA sequence

CTTGGATCATGGAGTTTTCTTCACTACTAATATTTCTACTGTTTTGGAGTTTTTTTCTTCCTTGTTTTACAGAAGAGAGAGAAATCTACTTGGTCTTAATGGAAGACGACGACCCAGTTGCCTTCCACCAAAGTGAGAGGACAACTAGTCCGAGCTTCGAAAGAAACAGCAAAGTTTCAAAAAAGGCAGCACCAAGAGAACTGGAGGGATTCCACGACCAGTTTCTTGAGAAAAGTCTAAGAGTGGAGAGCTACACGAAGCTTCACAGCTTCAAGCACATCATCAATGGGTTCGCGGTCCACACGACGCCATCGGAGGCGAGAAAGCTGAGAGAAGCTGAAGGGGTTGTGGCTGTGGAGAGAGACAGAGGAGTGAGGAAGATGACAACTTACACCCCTCAGTTTCTAGGGGTTCCCTCCACATTAAGGGCAACAGTGGGATGCAAGAGGAATGAAAGTAGTGGTGGAGAGGGGATTGTTATTGGGTTTGTGGACTCTGGGATTGACCCGACACACCCGAGTTTCGGTTTCGGTTTCGGTCTCGGTCTCGACGAGTCTTCTAAGTTTTGTGGGGTTTGTGAGGAAGGTCCTTTTTTTGGTTCAAATTCTTGCAATGGGAAGATTGTGGGAGCAAGATTTTTCTGTGCTGGGGCTCAGGCTGTTTCACAACTCAACTCTTCTGTGGATTTCCTCTCCCCTTTTGATGCTGAAGGCCATGGAAGCCACGTGGCATCTATAGCTGCTGGAAATGCAGGGGTTCCAGTCGTTGTTAATGGTTTTTTCTATGGATTGGCCAGTGGGATAGCGCCACGTGCACGAATTGCTATTTATAAGGCTGTGTATCCAACAGTGGCTACTCTAACAGATGTAGTTGCAGCCATAGATCAAGCAATTATTGATGGAGTTGATATATTGGCATTGTCTGTTGGACCAAATGAACCACCAGAAGTAGGATTTACGTTTCTAAGCATATATGACATTGCTGTATTATCTGCCACACGAGCCGGAATCCTTGTGGTTCAGGCTGCCGGAAATAACGGCCCGGCCCCGTCAACCGTCGTCTCTTACAGCCCTTGGGCTGTCGGCGTGGCCGCTTCCGGCACCGACCGAGTTTATCCGGCCTCACTTCTCCTTGGCAATGGCCAAAACGTTGGAGGCACAGGCTTGTCTGGACCCACTCTGGGGAGTGAGTTTTTGCTACATAAGCTGGTATTGGCCAAGGATGCAGTGAACCAAAATGTGGCACTTCCCACTGAAGAGTGCCAATATCCTCAAGCTTTTGACCCAAATATTGTCCAAAACAGCATAGTAATCTGCTCCTTCTCTCAAGGATTCCTCAATGGAACCTCCACCCTCACAGCTATCCTTCACACTGCAGCAACACTCAGATTCATGGCTTTTGTTCTCGTTGCAAATCCGAGCTATGGCGACTTCATTGCAGAACCCATCCCCTTCAGAATTCCTGGCATTCTCGTCCCGAGCGTTTCAGATTCTCAGGTGATATTGAAGTACTATGAGGAAAATATATGCAAGGATGGGAGGGGAATGGTTACAAACTTTAAAGGCAAAGCAGCCATAGGAGAAGGAAGATTTGCTTCATTTGGGGGCCAAGCTCCCACAGTTAGCAGATTTTCATCAAGAGGACCAGATTACATGAACAGTAATAGGACTGCAGCTGATGTGCTAAAGCCCGATATTCTCGCTCCAGGTCACCAAGTTTGGGCTGCTTGGAGCCCCCTTAGTGCCTCGGAGCCACTTTTAAAGGGATACCATTTTGCACTCTTGTCTGGAACAAGCATGGCAGCACCTCACATTGTGGGAATAGCAGCACTTATCAAGCAAAAGAATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCAATGTCCACAACTGCTACAAAGTATGATATGAATGGAGAGCTTATTCAGGCAGAGGGATATGGCATTCATAGTTTATATCCTTCTACTCCCTTTGATTTGGGCGCTGGCCTCGTCTCTCCGACCAATGCTTTAGACCCGGGATTGGTCTTCCCATCAGAATATGAAGACTATATAAACTTCTTGTGCTCTTTACCTGGCGTTGATCCTGCTGTCATCAAAAGCACCACTGGAAGACATTGCAATGCCTCTCTCTCACAGCCACACCCAGCAGATCTGAACCTTCCTTCACTCACAATATCATCACTGGTTGGGCAACAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGCAAAGTGGAGACATATGTGTGGTCGGTGATCCCGCCGAACGGGACAACAGTCGACATCAATCCGCCATGGTTCGCCATAGCGCCAGAAGGGGTCCAAAACTTGGAAATACAGATCAATGTCACACATAAAATGGATCACTTTAGCTTTGGGGAGATTGTTTTGACAGGAAGTTTAAACCATATTGCAAGGATCCCTTTGTCAGTTTTGGCTGTTTCTACATCCTAAAGGACAGGATTGGTTCATTTTCTTTGAAAGTCTCTCTATATTCCTTACTTTTAAAAGGGGGTAAAATTTTTCAAAAGATTATAATGTGTAAAATGTCAAATAAGTGATAGTTTCAAGATGAAACTAGTACACTAGTGTAGTTCTTAGAAATGAGCTCAAAAGTTTGAATATTACCAATATAGAAAGAAAGATCTTGTTGCTATAACATTTGAGAGCTAGTAGTTCTAATGGCAGACTGTTCTGTCTTTTTGTACAAAAAGGAAGTTTAGCCTGTCTTAAGAAAAAATCTTTTGAATGGTTTCTAAAGGTGAGATACAACTTTTCAATATCGATGGCGAATCTATATATAAATTCATGTGTAATTTCTGTTCTTTTAAATTTTAATTCATTTACAATTGTGGAACACAATTT

Coding sequence (CDS)

ATGGAGTTTTCTTCACTACTAATATTTCTACTGTTTTGGAGTTTTTTTCTTCCTTGTTTTACAGAAGAGAGAGAAATCTACTTGGTCTTAATGGAAGACGACGACCCAGTTGCCTTCCACCAAAGTGAGAGGACAACTAGTCCGAGCTTCGAAAGAAACAGCAAAGTTTCAAAAAAGGCAGCACCAAGAGAACTGGAGGGATTCCACGACCAGTTTCTTGAGAAAAGTCTAAGAGTGGAGAGCTACACGAAGCTTCACAGCTTCAAGCACATCATCAATGGGTTCGCGGTCCACACGACGCCATCGGAGGCGAGAAAGCTGAGAGAAGCTGAAGGGGTTGTGGCTGTGGAGAGAGACAGAGGAGTGAGGAAGATGACAACTTACACCCCTCAGTTTCTAGGGGTTCCCTCCACATTAAGGGCAACAGTGGGATGCAAGAGGAATGAAAGTAGTGGTGGAGAGGGGATTGTTATTGGGTTTGTGGACTCTGGGATTGACCCGACACACCCGAGTTTCGGTTTCGGTTTCGGTCTCGGTCTCGACGAGTCTTCTAAGTTTTGTGGGGTTTGTGAGGAAGGTCCTTTTTTTGGTTCAAATTCTTGCAATGGGAAGATTGTGGGAGCAAGATTTTTCTGTGCTGGGGCTCAGGCTGTTTCACAACTCAACTCTTCTGTGGATTTCCTCTCCCCTTTTGATGCTGAAGGCCATGGAAGCCACGTGGCATCTATAGCTGCTGGAAATGCAGGGGTTCCAGTCGTTGTTAATGGTTTTTTCTATGGATTGGCCAGTGGGATAGCGCCACGTGCACGAATTGCTATTTATAAGGCTGTGTATCCAACAGTGGCTACTCTAACAGATGTAGTTGCAGCCATAGATCAAGCAATTATTGATGGAGTTGATATATTGGCATTGTCTGTTGGACCAAATGAACCACCAGAAGTAGGATTTACGTTTCTAAGCATATATGACATTGCTGTATTATCTGCCACACGAGCCGGAATCCTTGTGGTTCAGGCTGCCGGAAATAACGGCCCGGCCCCGTCAACCGTCGTCTCTTACAGCCCTTGGGCTGTCGGCGTGGCCGCTTCCGGCACCGACCGAGTTTATCCGGCCTCACTTCTCCTTGGCAATGGCCAAAACGTTGGAGGCACAGGCTTGTCTGGACCCACTCTGGGGAGTGAGTTTTTGCTACATAAGCTGGTATTGGCCAAGGATGCAGTGAACCAAAATGTGGCACTTCCCACTGAAGAGTGCCAATATCCTCAAGCTTTTGACCCAAATATTGTCCAAAACAGCATAGTAATCTGCTCCTTCTCTCAAGGATTCCTCAATGGAACCTCCACCCTCACAGCTATCCTTCACACTGCAGCAACACTCAGATTCATGGCTTTTGTTCTCGTTGCAAATCCGAGCTATGGCGACTTCATTGCAGAACCCATCCCCTTCAGAATTCCTGGCATTCTCGTCCCGAGCGTTTCAGATTCTCAGGTGATATTGAAGTACTATGAGGAAAATATATGCAAGGATGGGAGGGGAATGGTTACAAACTTTAAAGGCAAAGCAGCCATAGGAGAAGGAAGATTTGCTTCATTTGGGGGCCAAGCTCCCACAGTTAGCAGATTTTCATCAAGAGGACCAGATTACATGAACAGTAATAGGACTGCAGCTGATGTGCTAAAGCCCGATATTCTCGCTCCAGGTCACCAAGTTTGGGCTGCTTGGAGCCCCCTTAGTGCCTCGGAGCCACTTTTAAAGGGATACCATTTTGCACTCTTGTCTGGAACAAGCATGGCAGCACCTCACATTGTGGGAATAGCAGCACTTATCAAGCAAAAGAATCCTTCTTGGACTCCTTCCATGATAGCTTCTGCAATGTCCACAACTGCTACAAAGTATGATATGAATGGAGAGCTTATTCAGGCAGAGGGATATGGCATTCATAGTTTATATCCTTCTACTCCCTTTGATTTGGGCGCTGGCCTCGTCTCTCCGACCAATGCTTTAGACCCGGGATTGGTCTTCCCATCAGAATATGAAGACTATATAAACTTCTTGTGCTCTTTACCTGGCGTTGATCCTGCTGTCATCAAAAGCACCACTGGAAGACATTGCAATGCCTCTCTCTCACAGCCACACCCAGCAGATCTGAACCTTCCTTCACTCACAATATCATCACTGGTTGGGCAACAGGTGGTGCAGAGAAGAGTGAAGAATGTAGGAGGCAAAGTGGAGACATATGTGTGGTCGGTGATCCCGCCGAACGGGACAACAGTCGACATCAATCCGCCATGGTTCGCCATAGCGCCAGAAGGGGTCCAAAACTTGGAAATACAGATCAATGTCACACATAAAATGGATCACTTTAGCTTTGGGGAGATTGTTTTGACAGGAAGTTTAAACCATATTGCAAGGATCCCTTTGTCAGTTTTGGCTGTTTCTACATCCTAA

Protein sequence

MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKAAPRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQYPQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS
Homology
BLAST of MC03g0533 vs. ExPASy Swiss-Prot
Match: F4HYR6 (Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 1.6e-258
Identity = 464/799 (58.07%), Postives = 577/799 (72.22%), Query Frame = 0

Query: 25  EIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKAAPRELEGFHDQFLEKSLRVESYTK 84
           +IY +L+E  +P+AF  S    S +    +K        ++E  HD+ L  +L   SYTK
Sbjct: 47  KIYSILVE-GEPLAFRASTNINSKAMALEAK--------KIEEIHDEILGSTLEKGSYTK 106

Query: 85  LHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVG 144
           L+SFKH+IN  AV TT S+A+KL + +GV AVE D+GV+ MTTYTP FL +P  +   + 
Sbjct: 107 LYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYTPDFLELPQQVWQKIS 166

Query: 145 CKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSK-----FCGVCEEGPFFGSN 204
               +   GE IVIGFVD+GI+PTHPSF     L    SS      F G CE GPFF   
Sbjct: 167 -NEGDRRAGEDIVIGFVDTGINPTHPSFA-ALDLTNPYSSNLSRLHFSGDCEIGPFFPPG 226

Query: 205 SCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFY 264
           SCNGKI+ ARFF AGA+A   LNSS+D LSPFDA GHGSHVASIAAGNAGVPV+V+GFFY
Sbjct: 227 SCNGKIISARFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAGNAGVPVIVDGFFY 286

Query: 265 GLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFL 324
           G ASG+APR+RIA+YKA+YP++ TL DV+AAIDQAI+DGVD+L LSVGP+EPP    T L
Sbjct: 287 GRASGMAPRSRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSVGPDEPPVDKPTVL 346

Query: 325 SIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQ 384
            I+D+A+L A +AG+ VVQA GNNGP+PS+V+SYSPW VGVAA  TDR YPA L+L  GQ
Sbjct: 347 GIFDLAMLLARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTDRSYPAPLILDGGQ 406

Query: 385 NVGGTGLSGPTLGSEFLLHKLVLAKDAVNQN------VALPTEECQYPQAFDPNIVQNSI 444
            V G GLSGPTLG+  + H+LVLAKDAV  N      +    EECQ P+ FDP  V  SI
Sbjct: 407 TVQGVGLSGPTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSI 466

Query: 445 VICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVS 504
           VIC+FS GF N  ST+ AI  TA TL FM F+L+ANP +GD++AEP+ F  PGIL+P+VS
Sbjct: 467 VICTFSDGFYNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVS 526

Query: 505 DSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNR 564
            +Q+IL+YYEE   +D RG+ T F  +A IGEGR + F G+AP VSRFSSRGP ++++ R
Sbjct: 527 AAQIILRYYEEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATR 586

Query: 565 TAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKN 624
           +  DVLKPDILAPGHQ+W AWS  SA +P+L G  FA+LSGTSMA PHI GI ALIKQ N
Sbjct: 587 SPLDVLKPDILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLN 646

Query: 625 PSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNALDPGLV 684
           PSWTP+MIASA+STTA +YD NGE+I AE Y +  L+PS  FD GAG V+P  ALDPGLV
Sbjct: 647 PSWTPAMIASAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLV 706

Query: 685 FPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQVVQ 744
            P+ +EDYI+FLCSLP + PA I+  TG  C  +LS  HPA+LN PS+TIS+L    VV+
Sbjct: 707 LPAGFEDYISFLCSLPNISPATIRDATGVLCTTTLS--HPANLNHPSVTISALKESLVVR 766

Query: 745 RRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFGEI 804
           R  ++V  K ETY+ SV+PPNGTTV + P WF + P+  Q+L+I+ NVT  ++ F+FGE+
Sbjct: 767 RSFQDVSNKTETYLGSVLPPNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEV 826

Query: 805 VLTGSLNHIARIPLSVLAV 813
           VLTGSLNHI RIPLSV  +
Sbjct: 827 VLTGSLNHIIRIPLSVKTI 832

BLAST of MC03g0533 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.8e-177
Identity = 351/799 (43.93%), Postives = 482/799 (60.33%), Query Frame = 0

Query: 25  EIYLVLMEDDDPVAFHQSER-TTSPSFERNSKVSKKAA-----PRELEGFHDQFLEKSLR 84
           E+Y+V ME D  +++   E    + + E + K+   +       R LE  HD  L     
Sbjct: 20  EVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHLERKHDMILGMLFE 79

Query: 85  VESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPST 144
             SY KL+S+KH+INGFA H +P +A  LR A GV +V++D  VR++TT+TP+FLG+P+ 
Sbjct: 80  EGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTD 139

Query: 145 LRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSKFCGVCEEGPFFGS 204
           +  T G        GE IVIGFVDSGI P HPSF     L       + G CEE P    
Sbjct: 140 VWPTGG---GFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKK 199

Query: 205 NSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFF 264
           + CN KIVGA+ F   A+A    N  +D+ SP D +GHGSH A+IAAGN G+P+ ++G+ 
Sbjct: 200 SFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHGYE 259

Query: 265 YGLASGIAPRARIAIYKAVYPTV-ATLTDVVAAIDQAIIDGVDILALSVGPNEPP-EVGF 324
           +G ASG+APRARIA+YKA+Y      + DVVAAIDQA+ DGVDIL+LSVGPN PP     
Sbjct: 260 FGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKT 319

Query: 325 TFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLG 384
           TFL+ +D  +L A +AG+ V QAAGN GP P T+VSYSPW   VAA+  DR Y   L LG
Sbjct: 320 TFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLG 379

Query: 385 NGQNVGGTGLSGPTLGSEFLLHKLVLAKDA-VNQNVA-LPTEECQYPQAFDPNIVQNSIV 444
           NG+ + G GLS PT      L+ LV A D  ++ +V+     +CQ P+ F+  +V+ +I+
Sbjct: 380 NGKMLAGMGLSPPT--RPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNKKLVEGNIL 439

Query: 445 ICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSD 504
           +C +S  F+ GT+++  ++ TA  L    FVLV          +P+P  IPGIL+  VS 
Sbjct: 440 LCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDVSK 499

Query: 505 SQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRT 564
           S  ++ YY  +  +D  G V +FK + +IG+G        AP V+ FS+RGP+  + +  
Sbjct: 500 SMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQ 559

Query: 565 AADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNP 624
            AD+LKPDILAPG+ +WAAW P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P
Sbjct: 560 DADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHP 619

Query: 625 SWTPSMIASAMSTTATKYDMNGELIQAEGYG---IHSLYPSTPFDLGAGLVSPTNALDPG 684
            W+P+ I SA+ TT+T  D  G L+QA+ Y      +L  +TPFD G+G V+P+ ALDPG
Sbjct: 620 QWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPG 679

Query: 685 LVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQV 744
           L+F + YEDY+ FLC+ PG+    I++ T   CN  +   HP++ N PS+ +S LVG Q 
Sbjct: 680 LIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMK--HPSNFNAPSIAVSHLVGTQT 739

Query: 745 VQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFG 804
           V R+V NV    ETY  +        +++NPP   + P   +   + + V      +SFG
Sbjct: 740 VTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFG 799

Query: 805 EIVLTGSLNHIARIPLSVL 811
           E+ L GS  H  RIP+  L
Sbjct: 800 EVKLKGSRGHKVRIPVVAL 811

BLAST of MC03g0533 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 1.5e-171
Identity = 350/807 (43.37%), Postives = 476/807 (58.98%), Query Frame = 0

Query: 16  FLPCF-TEEREIYLVLMEDDDPVAF----HQSERTTSPSFERNSKVSK--KAAPRELEGF 75
           F  CF T   EIY+V ME +  +++    +  E T   S E+    S+   +  R LE  
Sbjct: 10  FFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHLERK 69

Query: 76  HDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTY 135
           HD  L       SY KL+S+KH+INGFA H +P +A  LR A GV +V+RD  VRK+TT+
Sbjct: 70  HDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTH 129

Query: 136 TPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSF-GFGFGLGLDESSKFC 195
           TPQFLG+P+ +  T G        GE IVIGF+DSGI P HPSF      +       + 
Sbjct: 130 TPQFLGLPTDVWPTGG---GYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYK 189

Query: 196 GVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGN 255
           G CEE P    + CNGKI+GA+ F   A+A    N  +DF SP D +GHGSH A+IAAGN
Sbjct: 190 GKCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGN 249

Query: 256 AGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTV-ATLTDVVAAIDQAIIDGVDILALSV 315
            G+PV ++G+ +G ASG+APRARIA+YKA+Y      + DVVAAIDQA+ DGVDIL+LSV
Sbjct: 250 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSV 309

Query: 316 GPNEPP-EVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGT 375
           GPN PP     TFL+ +D  +L A +AG+ V QAAGN GP P T+VSYSPW   VAA+  
Sbjct: 310 GPNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAID 369

Query: 376 DRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAV--NQNVALPTEECQYPQA 435
           DR Y   L LGNG+ + G GLS  T       +K+V A D +  +  +     +CQ P+ 
Sbjct: 370 DRRYKNHLTLGNGKMLAGIGLSPST--RPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEV 429

Query: 436 FDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFR 495
            +  +V+ +I++C +S  F+ G++++  +  TA  L    FVLV          +P+P  
Sbjct: 430 LNKKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSC 489

Query: 496 IPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSS 555
           IPGIL+  VS S  ++ YY     +D  G V +FK + +IG+G        AP V+ FS+
Sbjct: 490 IPGILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSA 549

Query: 556 RGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIV 615
           RGP+  + +   AD+LKPDILAPG  +W+AWS     E    G  FAL+SGTSMAAPHI 
Sbjct: 550 RGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIA 609

Query: 616 GIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYG---IHSLYPSTPFDLGAG 675
           GIAAL+KQK+P W+P+ I SA+ TT+T  D  G  +QA+ Y      +L  +TPFD G+G
Sbjct: 610 GIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSG 669

Query: 676 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 735
            V+P+ ALDPGL+F + YEDYI FLC+ PG+D   IK+ T   CN      HP++ N PS
Sbjct: 670 HVNPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCN--FKMVHPSNFNTPS 729

Query: 736 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 795
           + IS LV  Q V RRV NV  + ETY  +        ++++PP   +     +   + + 
Sbjct: 730 IAISHLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLT 789

Query: 796 VTHKMDHFSFGEIVLTGSLNHIARIPL 808
           V      +SFG++ L GS  H   +P+
Sbjct: 790 VRSVTGAYSFGQVTLKGSRGHKVTLPV 809

BLAST of MC03g0533 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 4.5e-168
Identity = 348/792 (43.94%), Postives = 471/792 (59.47%), Query Frame = 0

Query: 40  HQSERTTSPSFERNS---KVSKKAAPRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFA 99
           H+  + T     RN+   +  K   P  ++  HD FL K+L+ E Y KL+S+ ++INGFA
Sbjct: 59  HKKSKFTPKLRPRNNSRKRHGKSKIPSVVQS-HDSFLRKTLKGEKYIKLYSYHYLINGFA 118

Query: 100 VHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGI 159
           +     +A KL   + V  +  D  VR  TTYTPQF+G+P       G        GEG+
Sbjct: 119 LFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWVKEG---GFEIAGEGV 178

Query: 160 VIGFVDSGIDPTHPSFGFGFGLGLDESSK--------FCGVCEEGPFFGSNSCNGKIVGA 219
           +IGF+D+GIDP HPSF        D  SK        F GVCE  P F S SCN K++GA
Sbjct: 179 IIGFIDTGIDPNHPSFN-------DNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGA 238

Query: 220 RFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFYGLASGIAPR 279
           R F   A      NSS D+ SPFD +GHG+H AS+AAGN GVPV+V+   +G ASGIAPR
Sbjct: 239 RHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPR 298

Query: 280 ARIAIYKAVYPTVATL-TDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFLSIYDIAVL 339
           A I++YKA+Y +      DVVAAIDQA  DGVDIL+LS+ PN  P    TF +  D+A+L
Sbjct: 299 AFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPPGVATFFNPIDMALL 358

Query: 340 SATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQNVGGTGLS 399
           SA +AGI VVQAAGN GPAP T+ S+SPW   V AS  DRVY  SL LGN   + G G +
Sbjct: 359 SAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFA 418

Query: 400 GPTLGSEFLLHKLVLAKDAVNQNVALPTE----ECQYPQAFDPNIVQNSIVICSFSQGFL 459
            PT   +  ++K++ A  A+N + ++  +    ECQ  + FD + V   ++ICS+S  F+
Sbjct: 419 IPTDSGK--MYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFV 478

Query: 460 NGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSDSQVILKYYE 519
            G ST+   L  A  L     +   +P    F   P P  +PGI++PSV DS+ +LKYY 
Sbjct: 479 LGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYN 538

Query: 520 ENICKD-GRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRTAADVLKPD 579
            +I +D     + +F   AAI  G  A+F  +AP V  +S+RGPD  +++   ADVLKP+
Sbjct: 539 SSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPN 598

Query: 580 ILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNPSWTPSMIA 639
           ++APG+ +W AWS  S      +G  FA++SGTSMAAPH+ G+AALIKQ  P +TPS I+
Sbjct: 599 LVAPGNSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTIS 658

Query: 640 SAMSTTATKYDMNGELIQAE---GYGIHSLYPSTPFDLGAGLVSPTNALDPGLVFPSEYE 699
           SA+STTA   D  G  I A+        SLY +TP D+G+G V+ T ALDPGLVF + +E
Sbjct: 659 SALSTTALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFE 718

Query: 700 DYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQVVQRRVKNV 759
           DYI+FLC + G D  V+ + TG  C A+ +     DLNLPS+T+S+L G Q  QR ++N+
Sbjct: 719 DYISFLCGINGSD-TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNI 778

Query: 760 GGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFGEIVLTGSL 812
            G  ETY     PP G ++ ++P  F+IA    Q L + + VT      SFG I L G+ 
Sbjct: 779 AGN-ETYNVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNT 835

BLAST of MC03g0533 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 2.3e-164
Identity = 331/758 (43.67%), Postives = 458/758 (60.42%), Query Frame = 0

Query: 69  HDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTY 128
           HD  L  +L+ E Y KL+SF ++INGFAV  +  +A  L     V  +  D  VR  TTY
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 129 TPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSK--- 188
           TPQF+G+P       G      + GEGIVIGF+D+GIDPTHPSF      G D S +   
Sbjct: 163 TPQFMGLPKGAWVKEG---GYETAGEGIVIGFIDTGIDPTHPSFN-----GTDTSQRQYP 222

Query: 189 ----FCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVA 248
               F GVCE  P F S SCN K+VGAR F   A      NSS D+ SPFD +GHG+H A
Sbjct: 223 IPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTA 282

Query: 249 SIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATL-TDVVAAIDQAIIDGVD 308
           SIAAGN GV  VV+G  +G ASGIAPRA I++YKA+Y +      DVVAAIDQA  DGVD
Sbjct: 283 SIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVD 342

Query: 309 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 368
           IL+LS+ PN  P    TF +  D+A+LSA +AGI VVQAAGN GP+P ++ S+SPW   V
Sbjct: 343 ILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTV 402

Query: 369 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTE---- 428
            A+  DR Y  S++LGN  ++ G GL+  T   E   + ++ A DA+    ++  +    
Sbjct: 403 GAASHDRDYSNSIVLGNNVSIPGVGLALRT--DEGKKYTMISALDALKNKSSVVDKDMYV 462

Query: 429 -ECQYPQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDF 488
            ECQ   +FD ++++ +++ICS+S  F+ G ST+   L  A  L     V   +P    F
Sbjct: 463 GECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGF 522

Query: 489 IAEPIPFRIPGILVPSVSDSQVILKYYEENICKDGRGM-VTNFKGKAAIGEGRFASFGGQ 548
              P P  +PGI++PS  DS+V+LKYY  ++ +DG    +  F   AAI  G+ A+F  +
Sbjct: 523 QINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNR 582

Query: 549 APTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSG 608
           AP +  +S+RGPD  +S    AD+LKP+++APG+ +W AWS  +      +G  FA++SG
Sbjct: 583 APKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSG 642

Query: 609 TSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAE---GYGIHSLYP 668
           TSMAAPH+ G+AAL+KQK   ++PS IASA+STT+  +D  GE I A+        ++ P
Sbjct: 643 TSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISP 702

Query: 669 STPFDLGAGLVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQP 728
           +TPFD+G G V+ T ALDPGL+F + +EDY++FLC + G  P V+ + TG +C  + +  
Sbjct: 703 ATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAP-VVFNYTGTNCLRNNATI 762

Query: 729 HPADLNLPSLTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEG 788
             +DLNLPS+T+S L   + VQR + N+ G  ETY  S+I P    ++++P  F+IA   
Sbjct: 763 SGSDLNLPSITVSKLNNTRTVQRLMTNIAGN-ETYTVSLITPFDVLINVSPTQFSIASGE 822

Query: 789 VQNLEIQINVTHKMDHFSFGEIVLTGSLNHIARIPLSV 810
            + L + +         SFG I L G+  HI RIP+SV
Sbjct: 823 TKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSV 848

BLAST of MC03g0533 vs. NCBI nr
Match: XP_022142659.1 (subtilisin-like protease SBT2.4 isoform X1 [Momordica charantia])

HSP 1 Score: 1602 bits (4148), Expect = 0.0
Identity = 810/815 (99.39%), Postives = 810/815 (99.39%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEFSSLLIFLLFWSFFLPCF EEREIYLVLMEDDDPVAFHQSERTT PSFERNSKVSKKA
Sbjct: 9   MEFSSLLIFLLFWSFFLPCFXEEREIYLVLMEDDDPVAFHQSERTTXPSFERNSKVSKKA 68

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
           APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR
Sbjct: 69  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 128

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL
Sbjct: 129 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 188

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV
Sbjct: 189 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 248

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGNAGVPVVVNGFFYGLASG APRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD
Sbjct: 249 ASIAAGNAGVPVVVNGFFYGLASGXAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 308

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV
Sbjct: 309 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 368

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY
Sbjct: 369 AASGTDRVYPASLLLGNGQXVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 428

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI
Sbjct: 429 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 488

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR
Sbjct: 489 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 548

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP
Sbjct: 549 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 608

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG
Sbjct: 609 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 668

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS
Sbjct: 669 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 728

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDI PPWFAIAPEGVQNLEIQIN
Sbjct: 729 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDIXPPWFAIAPEGVQNLEIQIN 788

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS
Sbjct: 789 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 823

BLAST of MC03g0533 vs. NCBI nr
Match: XP_022142660.1 (subtilisin-like protease SBT2.4 isoform X2 [Momordica charantia])

HSP 1 Score: 1595 bits (4131), Expect = 0.0
Identity = 809/815 (99.26%), Postives = 809/815 (99.26%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEFSSLLIFLLFWSFFLPCF EEREIYLVLMEDDDPVAFHQSERTT PSFE NSKVSKKA
Sbjct: 9   MEFSSLLIFLLFWSFFLPCFXEEREIYLVLMEDDDPVAFHQSERTTXPSFE-NSKVSKKA 68

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
           APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR
Sbjct: 69  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 128

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL
Sbjct: 129 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 188

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV
Sbjct: 189 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 248

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGNAGVPVVVNGFFYGLASG APRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD
Sbjct: 249 ASIAAGNAGVPVVVNGFFYGLASGXAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 308

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV
Sbjct: 309 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 368

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY
Sbjct: 369 AASGTDRVYPASLLLGNGQXVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 428

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI
Sbjct: 429 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 488

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR
Sbjct: 489 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 548

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP
Sbjct: 549 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 608

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG
Sbjct: 609 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 668

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS
Sbjct: 669 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 728

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDI PPWFAIAPEGVQNLEIQIN
Sbjct: 729 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDIXPPWFAIAPEGVQNLEIQIN 788

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS
Sbjct: 789 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 822

BLAST of MC03g0533 vs. NCBI nr
Match: XP_023523808.1 (subtilisin-like protease SBT2.4 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1214 bits (3141), Expect = 0.0
Identity = 626/815 (76.81%), Postives = 681/815 (83.56%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEF S L  L+     + CF EER+IYLVLME+                           
Sbjct: 29  MEFRSTLSVLIASLVLVYCFAEERQIYLVLMEESSN------------------------ 88

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
                    D  L+ +L+  SYTKL SFKHI+NGFAVHTTPSEA KLR+A GV  VERDR
Sbjct: 89  --------EDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDR 148

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTP+FLGVPS  R     K   S  GEGIV+GFVDSGIDPTHPSFGF    G 
Sbjct: 149 GVRKMTTYTPKFLGVPSKSRVC-SKKTKTSPDGEGIVVGFVDSGIDPTHPSFGFD--DGY 208

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           ++   FC   E+G FF  +SCNGKIVGARFF AGA+AV++LNSSVDFLSPFDAEGHGSHV
Sbjct: 209 EDLRLFC---EKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDAEGHGSHV 268

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGN GVPV VNGFFYG ASG+APRARIA+YKAVYPT++TLTDVV+AIDQA+IDGVD
Sbjct: 269 ASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVD 328

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPE G TFLSIYDIA+LSATRAGILVVQA GNNGPAP+TVVSYSPWAVGV
Sbjct: 329 ILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPAPATVVSYSPWAVGV 388

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGG GLSGPT    F LHKLVLAKDA+ QN  +P+EECQY
Sbjct: 389 AASGTDRVYPASLLLGNGQKVGGVGLSGPT----FFLHKLVLAKDAMKQNGTIPSEECQY 448

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           P+AFDPN+VQNSIV+CSFSQGFLNGTS+LTAI+HTA TLRF+ F L+ANP+YGDFIAEPI
Sbjct: 449 PEAFDPNLVQNSIVLCSFSQGFLNGTSSLTAIIHTATTLRFIGFALIANPNYGDFIAEPI 508

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PFR+PGIL+P+ SDSQ ILKYYE N CKD  GMV+ FKGKAAIGEGR ASFG QAPTVSR
Sbjct: 509 PFRLPGILIPNASDSQAILKYYEANTCKDANGMVSEFKGKAAIGEGRVASFGSQAPTVSR 568

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMN NRT ADVLKPDILAPGHQ+WAAWSPLS +EPLLKGY FAL+SGTSMAAP
Sbjct: 569 FSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAP 628

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNG+LIQAEG+ IH LYPSTPFDLGAG
Sbjct: 629 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGFDIHRLYPSTPFDLGAG 688

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LV PTNALDPGLVFP+EYEDYINFLCSLPG DPAVIK+TTG  CNAS  QPHPADLNLPS
Sbjct: 689 LVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNTTGGQCNASEPQPHPADLNLPS 748

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           +TISSLVG QV+ RRVKNVG KVETYVWSVIPPNGTT++INPPWF IAP G+QNLEIQI 
Sbjct: 749 VTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQIK 798

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VT KM+HF+FGEIV TGSLNHI RIPLSVLAVS S
Sbjct: 809 VTQKMNHFTFGEIVFTGSLNHIGRIPLSVLAVSVS 798

BLAST of MC03g0533 vs. NCBI nr
Match: XP_038895834.1 (subtilisin-like protease SBT2.4 [Benincasa hispida])

HSP 1 Score: 1214 bits (3141), Expect = 0.0
Identity = 620/762 (81.36%), Postives = 669/762 (87.80%), Query Frame = 0

Query: 64  ELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVR 123
           E E  H + LE +++  SYTKLHSFK I NGFAVHTTPS+A KLRE +GV  VE DRGVR
Sbjct: 2   EEEDSHSKLLENTIK--SYTKLHSFKKIFNGFAVHTTPSQASKLREVDGVKLVELDRGVR 61

Query: 124 KMTTYTPQFLG-VPSTLRATVGCKRNES---SGGEGIVIGFVDSGIDPTHPSFGFGFGLG 183
           KMTTYTP+FLG VPS +        N S    GGEGI+IGFVDSGI PTHPSF F     
Sbjct: 62  KMTTYTPEFLGLVPSNI-------HNYSIIDGGGEGILIGFVDSGIYPTHPSFSFFGNFD 121

Query: 184 LDESSKF-CGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGS 243
             E  +  CGVCEEGPFF   SCNGKIV ARFF AGAQAV++LNSS+DFLSPFDAEGHGS
Sbjct: 122 HQEEEELSCGVCEEGPFFPKASCNGKIVSARFFSAGAQAVAKLNSSLDFLSPFDAEGHGS 181

Query: 244 HVASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDG 303
           HVASIAAGNAGVPV+VNGFFYGLASGIAPRARIA+YKAVYPTVATL DV++AIDQA++DG
Sbjct: 182 HVASIAAGNAGVPVIVNGFFYGLASGIAPRARIAVYKAVYPTVATLIDVISAIDQAVVDG 241

Query: 304 VDILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAV 363
           VDILALSVGPNEPPEVGFTFLS YDIA+LSATRAGI+VVQAAGNNGPA +TVVSYSPWA+
Sbjct: 242 VDILALSVGPNEPPEVGFTFLSTYDIAILSATRAGIVVVQAAGNNGPARATVVSYSPWAI 301

Query: 364 GVAASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVA---LPT 423
           GVAASGTDRVY ASLLLGNGQ + G GLSGPTLGSEF LHKLVLAKD   QN     +P 
Sbjct: 302 GVAASGTDRVYSASLLLGNGQKIEGVGLSGPTLGSEFFLHKLVLAKDGKKQNETHSNIPN 361

Query: 424 --EECQYPQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYG 483
             EECQ P+AFDPNIVQNSIV+CSFSQGFLNGTS+L AI+HTA TLRFM FVL+ANP+YG
Sbjct: 362 YIEECQNPEAFDPNIVQNSIVLCSFSQGFLNGTSSLAAIIHTATTLRFMGFVLIANPNYG 421

Query: 484 DFIAEPIPFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGG 543
           DFIAEPIPFR+PGILVPSVSD+QVILKYYEEN CKDGRGMV+  KGKAAIGEGR ASFG 
Sbjct: 422 DFIAEPIPFRVPGILVPSVSDTQVILKYYEENTCKDGRGMVSEIKGKAAIGEGRVASFGR 481

Query: 544 QAPTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLS 603
           QAPTVSRFSSRGPDY+N NRT ADVLKPDILAPGHQ+WAAWSPLSASEPLLKGYHFALLS
Sbjct: 482 QAPTVSRFSSRGPDYININRTVADVLKPDILAPGHQIWAAWSPLSASEPLLKGYHFALLS 541

Query: 604 GTSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPST 663
           GTSMAAPHIVGIAALIKQK PSWTPSMIASAMSTTATKYDMNG+LIQAEG+ +H+LYPST
Sbjct: 542 GTSMAAPHIVGIAALIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNLHTLYPST 601

Query: 664 PFDLGAGLVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHP 723
           PFD GAGLVSPT+ALDPGLVFPSEY++YINFLCSLPGVDPAVIKS TG  CN  +SQPHP
Sbjct: 602 PFDFGAGLVSPTHALDPGLVFPSEYDEYINFLCSLPGVDPAVIKSATGGQCNTCVSQPHP 661

Query: 724 ADLNLPSLTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQ 783
           ADLNLPS+TISSLVG QVVQRRVKNVG KVETYVWSVIPPNGTTV+INPP F +APE VQ
Sbjct: 662 ADLNLPSVTISSLVGHQVVQRRVKNVGSKVETYVWSVIPPNGTTVNINPPRFTVAPEEVQ 721

Query: 784 NLEIQINVTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           NLEIQINVTHKMDHF+FGEI+LTGSLNHIARIPLSV AVS S
Sbjct: 722 NLEIQINVTHKMDHFTFGEIILTGSLNHIARIPLSVKAVSAS 754

BLAST of MC03g0533 vs. NCBI nr
Match: XP_022954879.1 (subtilisin-like protease SBT2.4 [Cucurbita moschata])

HSP 1 Score: 1208 bits (3126), Expect = 0.0
Identity = 624/815 (76.56%), Postives = 680/815 (83.44%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEF S+LI  L   +   CF EER+IYLVLME+                           
Sbjct: 29  MEFRSILIASLVLVY---CFAEERQIYLVLMEESSN------------------------ 88

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
                    D  L+ +L+  SYTKL SFKHI+NGFAVHTTPSEA KLR+A GV  VERDR
Sbjct: 89  --------EDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDR 148

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTP+FLGVPS  R     K   S  GEGIVIGFVDSGIDPTHPSFGF    G 
Sbjct: 149 GVRKMTTYTPKFLGVPSKSRVC-SKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFD--DGY 208

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           ++   FC   E+G FF  +SCNGKIVGARFF  GA+AV++LNSSVDFLSPFDAEGHGSHV
Sbjct: 209 EDLRLFC---EKGRFFPLSSCNGKIVGARFFSEGARAVAKLNSSVDFLSPFDAEGHGSHV 268

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGN GVPV VNGFFYG ASG+APRARIA+YKAVYPT++TLTDVV+AIDQA+IDGVD
Sbjct: 269 ASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVD 328

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPE G TFLSIYDIA+LSATRAGILVVQA GNNGPAP+TVVSYSPW+VGV
Sbjct: 329 ILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPAPATVVSYSPWSVGV 388

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGG GLSGPT    F LHKLVLAKDA+NQN  +P+EECQY
Sbjct: 389 AASGTDRVYPASLLLGNGQKVGGVGLSGPT----FFLHKLVLAKDAMNQNGTMPSEECQY 448

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           P+AFDPN+VQNSIV+CSFSQGFLNGTS+L AI+HTA TLRF+ F L+ANP+YGDFIAEPI
Sbjct: 449 PEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTATTLRFIGFALIANPNYGDFIAEPI 508

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PF +PGIL+P+ SDSQ ILKYYE N CKD  GMV+ FKGKAAIGEGR ASFG QAPTVSR
Sbjct: 509 PFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKAAIGEGRVASFGSQAPTVSR 568

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMN NRT ADVLKPDILAPGHQ+WAAWSPLS +EPLLKGY FAL+SGTSMAAP
Sbjct: 569 FSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAP 628

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNG+LIQAEG+ IH LYPSTPFDLGAG
Sbjct: 629 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNIHRLYPSTPFDLGAG 688

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LV PTNALDPGLVFP+EYEDYINFLCSLPG DPAVIK+TTG  CNAS  QPHPADLNLPS
Sbjct: 689 LVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNTTGGQCNASEPQPHPADLNLPS 748

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           +TISSLVG QV+ RRVKNVG KVETYVWSVIPPNGTT++INPPWF IAP G+QNLEIQI 
Sbjct: 749 VTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQIK 795

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VT KM+HF+FGEI+ TGSLNHI RIPLSVLAVS S
Sbjct: 809 VTQKMNHFTFGEIIFTGSLNHIGRIPLSVLAVSVS 795

BLAST of MC03g0533 vs. ExPASy TrEMBL
Match: A0A6J1CM39 (subtilisin-like protease SBT2.4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012715 PE=3 SV=1)

HSP 1 Score: 1602 bits (4148), Expect = 0.0
Identity = 810/815 (99.39%), Postives = 810/815 (99.39%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEFSSLLIFLLFWSFFLPCF EEREIYLVLMEDDDPVAFHQSERTT PSFERNSKVSKKA
Sbjct: 9   MEFSSLLIFLLFWSFFLPCFXEEREIYLVLMEDDDPVAFHQSERTTXPSFERNSKVSKKA 68

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
           APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR
Sbjct: 69  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 128

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL
Sbjct: 129 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 188

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV
Sbjct: 189 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 248

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGNAGVPVVVNGFFYGLASG APRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD
Sbjct: 249 ASIAAGNAGVPVVVNGFFYGLASGXAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 308

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV
Sbjct: 309 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 368

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY
Sbjct: 369 AASGTDRVYPASLLLGNGQXVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 428

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI
Sbjct: 429 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 488

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR
Sbjct: 489 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 548

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP
Sbjct: 549 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 608

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG
Sbjct: 609 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 668

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS
Sbjct: 669 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 728

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDI PPWFAIAPEGVQNLEIQIN
Sbjct: 729 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDIXPPWFAIAPEGVQNLEIQIN 788

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS
Sbjct: 789 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 823

BLAST of MC03g0533 vs. ExPASy TrEMBL
Match: A0A6J1CLJ5 (subtilisin-like protease SBT2.4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012715 PE=3 SV=1)

HSP 1 Score: 1595 bits (4131), Expect = 0.0
Identity = 809/815 (99.26%), Postives = 809/815 (99.26%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEFSSLLIFLLFWSFFLPCF EEREIYLVLMEDDDPVAFHQSERTT PSFE NSKVSKKA
Sbjct: 9   MEFSSLLIFLLFWSFFLPCFXEEREIYLVLMEDDDPVAFHQSERTTXPSFE-NSKVSKKA 68

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
           APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR
Sbjct: 69  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 128

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL
Sbjct: 129 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 188

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV
Sbjct: 189 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 248

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGNAGVPVVVNGFFYGLASG APRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD
Sbjct: 249 ASIAAGNAGVPVVVNGFFYGLASGXAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 308

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV
Sbjct: 309 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 368

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY
Sbjct: 369 AASGTDRVYPASLLLGNGQXVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 428

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI
Sbjct: 429 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 488

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR
Sbjct: 489 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 548

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP
Sbjct: 549 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 608

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG
Sbjct: 609 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 668

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS
Sbjct: 669 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 728

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDI PPWFAIAPEGVQNLEIQIN
Sbjct: 729 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDIXPPWFAIAPEGVQNLEIQIN 788

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS
Sbjct: 789 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 822

BLAST of MC03g0533 vs. ExPASy TrEMBL
Match: A0A6J1GUB5 (subtilisin-like protease SBT2.4 OS=Cucurbita moschata OX=3662 GN=LOC111457007 PE=3 SV=1)

HSP 1 Score: 1208 bits (3126), Expect = 0.0
Identity = 624/815 (76.56%), Postives = 680/815 (83.44%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEF S+LI  L   +   CF EER+IYLVLME+                           
Sbjct: 29  MEFRSILIASLVLVY---CFAEERQIYLVLMEESSN------------------------ 88

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
                    D  L+ +L+  SYTKL SFKHI+NGFAVHTTPSEA KLR+A GV  VERDR
Sbjct: 89  --------EDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDR 148

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTP+FLGVPS  R     K   S  GEGIVIGFVDSGIDPTHPSFGF    G 
Sbjct: 149 GVRKMTTYTPKFLGVPSKSRVC-SKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFD--DGY 208

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           ++   FC   E+G FF  +SCNGKIVGARFF  GA+AV++LNSSVDFLSPFDAEGHGSHV
Sbjct: 209 EDLRLFC---EKGRFFPLSSCNGKIVGARFFSEGARAVAKLNSSVDFLSPFDAEGHGSHV 268

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGN GVPV VNGFFYG ASG+APRARIA+YKAVYPT++TLTDVV+AIDQA+IDGVD
Sbjct: 269 ASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVD 328

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPE G TFLSIYDIA+LSATRAGILVVQA GNNGPAP+TVVSYSPW+VGV
Sbjct: 329 ILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPAPATVVSYSPWSVGV 388

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGG GLSGPT    F LHKLVLAKDA+NQN  +P+EECQY
Sbjct: 389 AASGTDRVYPASLLLGNGQKVGGVGLSGPT----FFLHKLVLAKDAMNQNGTMPSEECQY 448

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           P+AFDPN+VQNSIV+CSFSQGFLNGTS+L AI+HTA TLRF+ F L+ANP+YGDFIAEPI
Sbjct: 449 PEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTATTLRFIGFALIANPNYGDFIAEPI 508

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
           PF +PGIL+P+ SDSQ ILKYYE N CKD  GMV+ FKGKAAIGEGR ASFG QAPTVSR
Sbjct: 509 PFGLPGILIPNASDSQAILKYYEANTCKDDNGMVSEFKGKAAIGEGRVASFGSQAPTVSR 568

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMN NRT ADVLKPDILAPGHQ+WAAWSPLS +EPLLKGY FAL+SGTSMAAP
Sbjct: 569 FSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAP 628

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNG+LIQAEG+ IH LYPSTPFDLGAG
Sbjct: 629 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNIHRLYPSTPFDLGAG 688

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LV PTNALDPGLVFP+EYEDYINFLCSLPG DPAVIK+TTG  CNAS  QPHPADLNLPS
Sbjct: 689 LVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNTTGGQCNASEPQPHPADLNLPS 748

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           +TISSLVG QV+ RRVKNVG KVETYVWSVIPPNGTT++INPPWF IAP G+QNLEIQI 
Sbjct: 749 VTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQIK 795

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VT KM+HF+FGEI+ TGSLNHI RIPLSVLAVS S
Sbjct: 809 VTQKMNHFTFGEIIFTGSLNHIGRIPLSVLAVSVS 795

BLAST of MC03g0533 vs. ExPASy TrEMBL
Match: A0A6J1JWQ4 (subtilisin-like protease SBT2.4 OS=Cucurbita maxima OX=3661 GN=LOC111490380 PE=3 SV=1)

HSP 1 Score: 1205 bits (3117), Expect = 0.0
Identity = 622/815 (76.32%), Postives = 678/815 (83.19%), Query Frame = 0

Query: 1   MEFSSLLIFLLFWSFFLPCFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKA 60
           MEF S L  L+     + CF EER+IYLVLME+                           
Sbjct: 29  MEFRSTLSGLIASLVLVYCFAEERQIYLVLMEESSN------------------------ 88

Query: 61  APRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDR 120
                    D  L+ +L+  SYTKL SFKHI+NGFAVHTTPSEA KLR+A GV  VERDR
Sbjct: 89  --------EDHLLQNTLK--SYTKLQSFKHIMNGFAVHTTPSEAAKLRQANGVKLVERDR 148

Query: 121 GVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGL 180
           GVRKMTTYTP+FLGVPS  R     K   S  GEGIVIGFVDSGIDPTHPSFGF    G 
Sbjct: 149 GVRKMTTYTPKFLGVPSKSRLC-SKKTKTSPDGEGIVIGFVDSGIDPTHPSFGFD--DGY 208

Query: 181 DESSKFCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHV 240
           ++   FC   E+G FF  +SCNGKIVGARFF AGA+AV++LNSSVDFLSPFD EGHGSHV
Sbjct: 209 EDLRLFC---EKGRFFPLSSCNGKIVGARFFSAGARAVAKLNSSVDFLSPFDVEGHGSHV 268

Query: 241 ASIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVD 300
           ASIAAGN GVPV VNGFFYG ASG+APRARIA+YKAVYPT++TLTDVV+AIDQA+IDGVD
Sbjct: 269 ASIAAGNGGVPVYVNGFFYGFASGMAPRARIAVYKAVYPTMSTLTDVVSAIDQAVIDGVD 328

Query: 301 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 360
           ILALSVGPNEPPE G TFLSIYDIA+LSATRAGILVVQA GNNGPA +TVVSYSPWAVGV
Sbjct: 329 ILALSVGPNEPPEEGLTFLSIYDIAILSATRAGILVVQAVGNNGPASATVVSYSPWAVGV 388

Query: 361 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTEECQY 420
           AASGTDRVYPASLLLGNGQ VGG GLSGPT    F LHKLVLAKDA+ QN  +P+EECQY
Sbjct: 389 AASGTDRVYPASLLLGNGQKVGGVGLSGPT----FFLHKLVLAKDAMKQNGTIPSEECQY 448

Query: 421 PQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPI 480
           P+AFDPN+VQNSIV+CSFSQGFLNGTS+L AI+HTA TLRF+ F L+ANP+YGDFIAEPI
Sbjct: 449 PEAFDPNLVQNSIVLCSFSQGFLNGTSSLAAIIHTAMTLRFIGFALIANPNYGDFIAEPI 508

Query: 481 PFRIPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSR 540
            FR+PGIL+P+ SDSQ IL YYE N CKD  GMV+ FKGKAAIGEGR ASFG QAPTVSR
Sbjct: 509 TFRLPGILIPNASDSQAILNYYEANTCKDANGMVSEFKGKAAIGEGRVASFGSQAPTVSR 568

Query: 541 FSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAP 600
           FSSRGPDYMN NRT ADVLKPDILAPGHQ+WAAWSPLS +EPLLKGY FAL+SGTSMAAP
Sbjct: 569 FSSRGPDYMNMNRTVADVLKPDILAPGHQIWAAWSPLSVTEPLLKGYQFALMSGTSMAAP 628

Query: 601 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAG 660
           HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNG+LIQAEG+ IH LYPSTPFDLGAG
Sbjct: 629 HIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGDLIQAEGFDIHRLYPSTPFDLGAG 688

Query: 661 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 720
           LV PTNALDPGLVFP+EYEDYINFLCSLPG DPAVIK+TTG  CNAS+ QPHPADLNLPS
Sbjct: 689 LVKPTNALDPGLVFPAEYEDYINFLCSLPG-DPAVIKNTTGGQCNASVPQPHPADLNLPS 748

Query: 721 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 780
           +TISSLVG QV+ RRVKNVG KVETYVWSVIPPNGTT++INPPWF IAP G+QNLEIQI 
Sbjct: 749 VTISSLVGHQVLHRRVKNVGTKVETYVWSVIPPNGTTININPPWFTIAPGGLQNLEIQIK 798

Query: 781 VTHKMDHFSFGEIVLTGSLNHIARIPLSVLAVSTS 815
           VTHKM+HF+FGEI+ TGSLNHI RIPLSVLAVS S
Sbjct: 809 VTHKMNHFTFGEILFTGSLNHIGRIPLSVLAVSVS 798

BLAST of MC03g0533 vs. ExPASy TrEMBL
Match: A0A1S3C9G9 (LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=LOC103498334 PE=3 SV=1)

HSP 1 Score: 1187 bits (3072), Expect = 0.0
Identity = 608/806 (75.43%), Postives = 675/806 (83.75%), Query Frame = 0

Query: 19  CFTEEREIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKAAPRELEGFHDQFLEKSLR 78
           C+ E+REI+LV+MEDD       S+   S                 +E  H + LE S++
Sbjct: 13  CYAEKREIFLVVMEDDGGYELRNSKEMLS-----------------IEDSHKKVLENSIK 72

Query: 79  VESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPST 138
             +Y KLHSFK I+NGFAVHTTPSEA KLREA GV  VE DRGV+KMTTYTP+FLG+   
Sbjct: 73  --NYRKLHSFKKILNGFAVHTTPSEASKLREANGVKLVELDRGVKKMTTYTPEFLGL--- 132

Query: 139 LRATVGCKRNES-----SGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSKFCGVCEEG 198
           ++     K N S     +GG+GI+IGFVDSGI P HPSF   FG   D+  +   VCEEG
Sbjct: 133 VKNNNDYKYNYSGGISGAGGDGILIGFVDSGIYPKHPSFSNNFGKDDDDYGEL--VCEEG 192

Query: 199 PFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVV 258
           P F    CNGKIV A FF AGAQA + LNSS+DFLSPFDAEGHGSHVASIAAGNA VPV+
Sbjct: 193 PLFPKGCCNGKIVSAMFFSAGAQAAAVLNSSIDFLSPFDAEGHGSHVASIAAGNAEVPVI 252

Query: 259 VNGFFYGLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVDILALSVGPNEPPE 318
           V+GFFYGLASGIAP ARI +YKAVYPT+ATLTDV++AIDQA+IDGVDILALSVGPNEPPE
Sbjct: 253 VDGFFYGLASGIAPHARIGVYKAVYPTIATLTDVISAIDQAVIDGVDILALSVGPNEPPE 312

Query: 319 VGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASL 378
           VGFTFLSIYDIA+LSATRAGILVVQAAGNNGPA +TVVSYSPWA+GVAASGTDRVY +SL
Sbjct: 313 VGFTFLSIYDIAILSATRAGILVVQAAGNNGPARATVVSYSPWAIGVAASGTDRVYSSSL 372

Query: 379 LLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVA----LPT--EECQYPQAFDPN 438
           LLGNGQ +GG G+SGP+LGSEF LHKLVLAKDA  QN      +P+  EECQ P+AFDPN
Sbjct: 373 LLGNGQKIGGVGMSGPSLGSEFFLHKLVLAKDAKMQNETQYDDIPSYIEECQNPEAFDPN 432

Query: 439 IVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGI 498
           IVQNSIV+CSFSQGFLNGTS+L AI+HTA  L+FM FVL+ANP+YGDFIAEPIPFR+PGI
Sbjct: 433 IVQNSIVLCSFSQGFLNGTSSLAAIIHTAKKLKFMGFVLIANPNYGDFIAEPIPFRVPGI 492

Query: 499 LVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPD 558
           LVPSVSD+QVILKYYEEN CKDGRGMV  FKGKA IGEGR ASFG +APTVSRFSSRGPD
Sbjct: 493 LVPSVSDTQVILKYYEENTCKDGRGMVREFKGKAGIGEGRIASFGKEAPTVSRFSSRGPD 552

Query: 559 YMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAA 618
           Y+N NR+ ADVLKPDILAPGHQ+WAAWSPLSASEPLLKGY FAL+SGTSMAAPHIVG+AA
Sbjct: 553 YININRSLADVLKPDILAPGHQIWAAWSPLSASEPLLKGYRFALMSGTSMAAPHIVGVAA 612

Query: 619 LIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNA 678
           LIKQK PSWTPSMIASAMSTTATKYDMNG+LIQAEG+ +H+LYPSTPFD GAGLVSPTNA
Sbjct: 613 LIKQKYPSWTPSMIASAMSTTATKYDMNGDLIQAEGFNLHALYPSTPFDFGAGLVSPTNA 672

Query: 679 LDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLV 738
           LDPGLVFP+EYEDYINFLCSLPGVDPAV+KS TG  CNAS+S  HPADLNLPS+TISSLV
Sbjct: 673 LDPGLVFPTEYEDYINFLCSLPGVDPAVVKSATGGQCNASISHSHPADLNLPSITISSLV 732

Query: 739 GQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDH 798
           G QVV+RRVKNVGGKVETYV SVIPPNGTTV+I PPWF +A E VQNLEIQI  THK DH
Sbjct: 733 GHQVVRRRVKNVGGKVETYVCSVIPPNGTTVNIKPPWFTVAAEEVQNLEIQIIATHKTDH 792

Query: 799 FSFGEIVLTGSLNHIARIPLSVLAVS 813
           F+FGEI LTGSLNHIARIPLS+L VS
Sbjct: 793 FTFGEIFLTGSLNHIARIPLSILVVS 794

BLAST of MC03g0533 vs. TAIR 10
Match: AT1G62340.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 893.6 bits (2308), Expect = 1.1e-259
Identity = 464/799 (58.07%), Postives = 577/799 (72.22%), Query Frame = 0

Query: 25  EIYLVLMEDDDPVAFHQSERTTSPSFERNSKVSKKAAPRELEGFHDQFLEKSLRVESYTK 84
           +IY +L+E  +P+AF  S    S +    +K        ++E  HD+ L  +L   SYTK
Sbjct: 47  KIYSILVE-GEPLAFRASTNINSKAMALEAK--------KIEEIHDEILGSTLEKGSYTK 106

Query: 85  LHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVG 144
           L+SFKH+IN  AV TT S+A+KL + +GV AVE D+GV+ MTTYTP FL +P  +   + 
Sbjct: 107 LYSFKHVINAIAVRTTASQAKKLGKTKGVKAVEEDKGVKLMTTYTPDFLELPQQVWQKIS 166

Query: 145 CKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSK-----FCGVCEEGPFFGSN 204
               +   GE IVIGFVD+GI+PTHPSF     L    SS      F G CE GPFF   
Sbjct: 167 -NEGDRRAGEDIVIGFVDTGINPTHPSFA-ALDLTNPYSSNLSRLHFSGDCEIGPFFPPG 226

Query: 205 SCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFY 264
           SCNGKI+ ARFF AGA+A   LNSS+D LSPFDA GHGSHVASIAAGNAGVPV+V+GFFY
Sbjct: 227 SCNGKIISARFFSAGARASGALNSSLDILSPFDASGHGSHVASIAAGNAGVPVIVDGFFY 286

Query: 265 GLASGIAPRARIAIYKAVYPTVATLTDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFL 324
           G ASG+APR+RIA+YKA+YP++ TL DV+AAIDQAI+DGVD+L LSVGP+EPP    T L
Sbjct: 287 GRASGMAPRSRIAVYKAIYPSIGTLVDVIAAIDQAIMDGVDVLTLSVGPDEPPVDKPTVL 346

Query: 325 SIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQ 384
            I+D+A+L A +AG+ VVQA GNNGP+PS+V+SYSPW VGVAA  TDR YPA L+L  GQ
Sbjct: 347 GIFDLAMLLARKAGVFVVQAVGNNGPSPSSVLSYSPWVVGVAAGNTDRSYPAPLILDGGQ 406

Query: 385 NVGGTGLSGPTLGSEFLLHKLVLAKDAVNQN------VALPTEECQYPQAFDPNIVQNSI 444
            V G GLSGPTLG+  + H+LVLAKDAV  N      +    EECQ P+ FDP  V  SI
Sbjct: 407 TVQGVGLSGPTLGAPLVQHRLVLAKDAVRTNGSVLQPLTRDIEECQRPENFDPAAVFGSI 466

Query: 445 VICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVS 504
           VIC+FS GF N  ST+ AI  TA TL FM F+L+ANP +GD++AEP+ F  PGIL+P+VS
Sbjct: 467 VICTFSDGFYNQMSTVLAITQTARTLGFMGFILIANPRFGDYVAEPVIFSAPGILIPTVS 526

Query: 505 DSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNR 564
            +Q+IL+YYEE   +D RG+ T F  +A IGEGR + F G+AP VSRFSSRGP ++++ R
Sbjct: 527 AAQIILRYYEEKTFRDTRGVATQFGARARIGEGRNSVFAGKAPVVSRFSSRGPAFIDATR 586

Query: 565 TAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKN 624
           +  DVLKPDILAPGHQ+W AWS  SA +P+L G  FA+LSGTSMA PHI GI ALIKQ N
Sbjct: 587 SPLDVLKPDILAPGHQIWGAWSLPSAFDPILTGRSFAILSGTSMATPHIAGIGALIKQLN 646

Query: 625 PSWTPSMIASAMSTTATKYDMNGELIQAEGYGIHSLYPSTPFDLGAGLVSPTNALDPGLV 684
           PSWTP+MIASA+STTA +YD NGE+I AE Y +  L+PS  FD GAG V+P  ALDPGLV
Sbjct: 647 PSWTPAMIASAISTTANEYDSNGEIISAEYYELSRLFPSNHFDHGAGHVNPARALDPGLV 706

Query: 685 FPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQVVQ 744
            P+ +EDYI+FLCSLP + PA I+  TG  C  +LS  HPA+LN PS+TIS+L    VV+
Sbjct: 707 LPAGFEDYISFLCSLPNISPATIRDATGVLCTTTLS--HPANLNHPSVTISALKESLVVR 766

Query: 745 RRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFGEI 804
           R  ++V  K ETY+ SV+PPNGTTV + P WF + P+  Q+L+I+ NVT  ++ F+FGE+
Sbjct: 767 RSFQDVSNKTETYLGSVLPPNGTTVRLTPTWFTVPPQKTQDLDIEFNVTQVLNKFTFGEV 826

Query: 805 VLTGSLNHIARIPLSVLAV 813
           VLTGSLNHI RIPLSV  +
Sbjct: 827 VLTGSLNHIIRIPLSVKTI 832

BLAST of MC03g0533 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 624.4 bits (1609), Expect = 1.3e-178
Identity = 351/799 (43.93%), Postives = 482/799 (60.33%), Query Frame = 0

Query: 25  EIYLVLMEDDDPVAFHQSER-TTSPSFERNSKVSKKAA-----PRELEGFHDQFLEKSLR 84
           E+Y+V ME D  +++   E    + + E + K+   +       R LE  HD  L     
Sbjct: 20  EVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHLERKHDMILGMLFE 79

Query: 85  VESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPST 144
             SY KL+S+KH+INGFA H +P +A  LR A GV +V++D  VR++TT+TP+FLG+P+ 
Sbjct: 80  EGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLGLPTD 139

Query: 145 LRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSKFCGVCEEGPFFGS 204
           +  T G        GE IVIGFVDSGI P HPSF     L       + G CEE P    
Sbjct: 140 VWPTGG---GFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKGKCEEDPHTKK 199

Query: 205 NSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFF 264
           + CN KIVGA+ F   A+A    N  +D+ SP D +GHGSH A+IAAGN G+P+ ++G+ 
Sbjct: 200 SFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHGYE 259

Query: 265 YGLASGIAPRARIAIYKAVYPTV-ATLTDVVAAIDQAIIDGVDILALSVGPNEPP-EVGF 324
           +G ASG+APRARIA+YKA+Y      + DVVAAIDQA+ DGVDIL+LSVGPN PP     
Sbjct: 260 FGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTTKT 319

Query: 325 TFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLG 384
           TFL+ +D  +L A +AG+ V QAAGN GP P T+VSYSPW   VAA+  DR Y   L LG
Sbjct: 320 TFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLTLG 379

Query: 385 NGQNVGGTGLSGPTLGSEFLLHKLVLAKDA-VNQNVA-LPTEECQYPQAFDPNIVQNSIV 444
           NG+ + G GLS PT      L+ LV A D  ++ +V+     +CQ P+ F+  +V+ +I+
Sbjct: 380 NGKMLAGMGLSPPT--RPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNKKLVEGNIL 439

Query: 445 ICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSD 504
           +C +S  F+ GT+++  ++ TA  L    FVLV          +P+P  IPGIL+  VS 
Sbjct: 440 LCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDVSK 499

Query: 505 SQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRT 564
           S  ++ YY  +  +D  G V +FK + +IG+G        AP V+ FS+RGP+  + +  
Sbjct: 500 SMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDFSFQ 559

Query: 565 AADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNP 624
            AD+LKPDILAPG+ +WAAW P    EP   G  FAL+SGTSMAAPHI GIAAL+KQK+P
Sbjct: 560 DADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIAALVKQKHP 619

Query: 625 SWTPSMIASAMSTTATKYDMNGELIQAEGYG---IHSLYPSTPFDLGAGLVSPTNALDPG 684
            W+P+ I SA+ TT+T  D  G L+QA+ Y      +L  +TPFD G+G V+P+ ALDPG
Sbjct: 620 QWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVNPSAALDPG 679

Query: 685 LVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQV 744
           L+F + YEDY+ FLC+ PG+    I++ T   CN  +   HP++ N PS+ +S LVG Q 
Sbjct: 680 LIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMK--HPSNFNAPSIAVSHLVGTQT 739

Query: 745 VQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFG 804
           V R+V NV    ETY  +        +++NPP   + P   +   + + V      +SFG
Sbjct: 740 VTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVSGVYSFG 799

Query: 805 EIVLTGSLNHIARIPLSVL 811
           E+ L GS  H  RIP+  L
Sbjct: 800 EVKLKGSRGHKVRIPVVAL 811

BLAST of MC03g0533 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 604.7 bits (1558), Expect = 1.1e-172
Identity = 350/807 (43.37%), Postives = 476/807 (58.98%), Query Frame = 0

Query: 16  FLPCF-TEEREIYLVLMEDDDPVAF----HQSERTTSPSFERNSKVSK--KAAPRELEGF 75
           F  CF T   EIY+V ME +  +++    +  E T   S E+    S+   +  R LE  
Sbjct: 10  FFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHLERK 69

Query: 76  HDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTY 135
           HD  L       SY KL+S+KH+INGFA H +P +A  LR A GV +V+RD  VRK+TT+
Sbjct: 70  HDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTH 129

Query: 136 TPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSF-GFGFGLGLDESSKFC 195
           TPQFLG+P+ +  T G        GE IVIGF+DSGI P HPSF      +       + 
Sbjct: 130 TPQFLGLPTDVWPTGG---GYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYK 189

Query: 196 GVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGN 255
           G CEE P    + CNGKI+GA+ F   A+A    N  +DF SP D +GHGSH A+IAAGN
Sbjct: 190 GKCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGN 249

Query: 256 AGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTV-ATLTDVVAAIDQAIIDGVDILALSV 315
            G+PV ++G+ +G ASG+APRARIA+YKA+Y      + DVVAAIDQA+ DGVDIL+LSV
Sbjct: 250 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSV 309

Query: 316 GPNEPP-EVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGT 375
           GPN PP     TFL+ +D  +L A +AG+ V QAAGN GP P T+VSYSPW   VAA+  
Sbjct: 310 GPNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAID 369

Query: 376 DRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAV--NQNVALPTEECQYPQA 435
           DR Y   L LGNG+ + G GLS  T       +K+V A D +  +  +     +CQ P+ 
Sbjct: 370 DRRYKNHLTLGNGKMLAGIGLSPST--RPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEV 429

Query: 436 FDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFR 495
            +  +V+ +I++C +S  F+ G++++  +  TA  L    FVLV          +P+P  
Sbjct: 430 LNKKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSC 489

Query: 496 IPGILVPSVSDSQVILKYYEENICKDGRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSS 555
           IPGIL+  VS S  ++ YY     +D  G V +FK + +IG+G        AP V+ FS+
Sbjct: 490 IPGILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSA 549

Query: 556 RGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIV 615
           RGP+  + +   AD+LKPDILAPG  +W+AWS     E    G  FAL+SGTSMAAPHI 
Sbjct: 550 RGPNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIA 609

Query: 616 GIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAEGYG---IHSLYPSTPFDLGAG 675
           GIAAL+KQK+P W+P+ I SA+ TT+T  D  G  +QA+ Y      +L  +TPFD G+G
Sbjct: 610 GIAALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSG 669

Query: 676 LVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPS 735
            V+P+ ALDPGL+F + YEDYI FLC+ PG+D   IK+ T   CN      HP++ N PS
Sbjct: 670 HVNPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCN--FKMVHPSNFNTPS 729

Query: 736 LTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQIN 795
           + IS LV  Q V RRV NV  + ETY  +        ++++PP   +     +   + + 
Sbjct: 730 IAISHLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLT 789

Query: 796 VTHKMDHFSFGEIVLTGSLNHIARIPL 808
           V      +SFG++ L GS  H   +P+
Sbjct: 790 VRSVTGAYSFGQVTLKGSRGHKVTLPV 809

BLAST of MC03g0533 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 593.2 bits (1528), Expect = 3.2e-169
Identity = 348/792 (43.94%), Postives = 471/792 (59.47%), Query Frame = 0

Query: 40  HQSERTTSPSFERNS---KVSKKAAPRELEGFHDQFLEKSLRVESYTKLHSFKHIINGFA 99
           H+  + T     RN+   +  K   P  ++  HD FL K+L+ E Y KL+S+ ++INGFA
Sbjct: 59  HKKSKFTPKLRPRNNSRKRHGKSKIPSVVQS-HDSFLRKTLKGEKYIKLYSYHYLINGFA 118

Query: 100 VHTTPSEARKLREAEGVVAVERDRGVRKMTTYTPQFLGVPSTLRATVGCKRNESSGGEGI 159
           +     +A KL   + V  +  D  VR  TTYTPQF+G+P       G        GEG+
Sbjct: 119 LFINSQQAEKLSMRKEVANIVLDYSVRTATTYTPQFMGLPQGAWVKEG---GFEIAGEGV 178

Query: 160 VIGFVDSGIDPTHPSFGFGFGLGLDESSK--------FCGVCEEGPFFGSNSCNGKIVGA 219
           +IGF+D+GIDP HPSF        D  SK        F GVCE  P F S SCN K++GA
Sbjct: 179 IIGFIDTGIDPNHPSFN-------DNDSKRSYPIPKHFSGVCEVTPDFPSGSCNKKLIGA 238

Query: 220 RFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVASIAAGNAGVPVVVNGFFYGLASGIAPR 279
           R F   A      NSS D+ SPFD +GHG+H AS+AAGN GVPV+V+   +G ASGIAPR
Sbjct: 239 RHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHGVPVIVSNHNFGYASGIAPR 298

Query: 280 ARIAIYKAVYPTVATL-TDVVAAIDQAIIDGVDILALSVGPNEPPEVGFTFLSIYDIAVL 339
           A I++YKA+Y +      DVVAAIDQA  DGVDIL+LS+ PN  P    TF +  D+A+L
Sbjct: 299 AFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRKPPGVATFFNPIDMALL 358

Query: 340 SATRAGILVVQAAGNNGPAPSTVVSYSPWAVGVAASGTDRVYPASLLLGNGQNVGGTGLS 399
           SA +AGI VVQAAGN GPAP T+ S+SPW   V AS  DRVY  SL LGN   + G G +
Sbjct: 359 SAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRVYSNSLTLGNNVTIPGMGFA 418

Query: 400 GPTLGSEFLLHKLVLAKDAVNQNVALPTE----ECQYPQAFDPNIVQNSIVICSFSQGFL 459
            PT   +  ++K++ A  A+N + ++  +    ECQ  + FD + V   ++ICS+S  F+
Sbjct: 419 IPTDSGK--MYKMISAFHALNNSTSVDKDMYVGECQDYENFDQDRVSGKLLICSYSARFV 478

Query: 460 NGTSTLTAILHTAATLRFMAFVLVANPSYGDFIAEPIPFRIPGILVPSVSDSQVILKYYE 519
            G ST+   L  A  L     +   +P    F   P P  +PGI++PSV DS+ +LKYY 
Sbjct: 479 LGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPGIIIPSVEDSKTLLKYYN 538

Query: 520 ENICKD-GRGMVTNFKGKAAIGEGRFASFGGQAPTVSRFSSRGPDYMNSNRTAADVLKPD 579
            +I +D     + +F   AAI  G  A+F  +AP V  +S+RGPD  +++   ADVLKP+
Sbjct: 539 SSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARGPDPEDNSFNDADVLKPN 598

Query: 580 ILAPGHQVWAAWSPLSASEPLLKGYHFALLSGTSMAAPHIVGIAALIKQKNPSWTPSMIA 639
           ++APG+ +W AWS  S      +G  FA++SGTSMAAPH+ G+AALIKQ  P +TPS I+
Sbjct: 599 LVAPGNSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAGVAALIKQSYPQFTPSTIS 658

Query: 640 SAMSTTATKYDMNGELIQAE---GYGIHSLYPSTPFDLGAGLVSPTNALDPGLVFPSEYE 699
           SA+STTA   D  G  I A+        SLY +TP D+G+G V+ T ALDPGLVF + +E
Sbjct: 659 SALSTTALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGFVNATAALDPGLVFDTSFE 718

Query: 700 DYINFLCSLPGVDPAVIKSTTGRHCNASLSQPHPADLNLPSLTISSLVGQQVVQRRVKNV 759
           DYI+FLC + G D  V+ + TG  C A+ +     DLNLPS+T+S+L G Q  QR ++N+
Sbjct: 719 DYISFLCGINGSD-TVVFNYTGFRCPANNTPVSGFDLNLPSITVSTLSGTQTFQRSMRNI 778

Query: 760 GGKVETYVWSVIPPNGTTVDINPPWFAIAPEGVQNLEIQINVTHKMDHFSFGEIVLTGSL 812
            G  ETY     PP G ++ ++P  F+IA    Q L + + VT      SFG I L G+ 
Sbjct: 779 AGN-ETYNVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLTVTKNSSSSSFGRIGLFGNT 835

BLAST of MC03g0533 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.6e-165
Identity = 331/758 (43.67%), Postives = 458/758 (60.42%), Query Frame = 0

Query: 69  HDQFLEKSLRVESYTKLHSFKHIINGFAVHTTPSEARKLREAEGVVAVERDRGVRKMTTY 128
           HD  L  +L+ E Y KL+SF ++INGFAV  +  +A  L     V  +  D  VR  TTY
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 129 TPQFLGVPSTLRATVGCKRNESSGGEGIVIGFVDSGIDPTHPSFGFGFGLGLDESSK--- 188
           TPQF+G+P       G      + GEGIVIGF+D+GIDPTHPSF      G D S +   
Sbjct: 163 TPQFMGLPKGAWVKEG---GYETAGEGIVIGFIDTGIDPTHPSFN-----GTDTSQRQYP 222

Query: 189 ----FCGVCEEGPFFGSNSCNGKIVGARFFCAGAQAVSQLNSSVDFLSPFDAEGHGSHVA 248
               F GVCE  P F S SCN K+VGAR F   A      NSS D+ SPFD +GHG+H A
Sbjct: 223 IPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTA 282

Query: 249 SIAAGNAGVPVVVNGFFYGLASGIAPRARIAIYKAVYPTVATL-TDVVAAIDQAIIDGVD 308
           SIAAGN GV  VV+G  +G ASGIAPRA I++YKA+Y +      DVVAAIDQA  DGVD
Sbjct: 283 SIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVD 342

Query: 309 ILALSVGPNEPPEVGFTFLSIYDIAVLSATRAGILVVQAAGNNGPAPSTVVSYSPWAVGV 368
           IL+LS+ PN  P    TF +  D+A+LSA +AGI VVQAAGN GP+P ++ S+SPW   V
Sbjct: 343 ILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTV 402

Query: 369 AASGTDRVYPASLLLGNGQNVGGTGLSGPTLGSEFLLHKLVLAKDAVNQNVALPTE---- 428
            A+  DR Y  S++LGN  ++ G GL+  T   E   + ++ A DA+    ++  +    
Sbjct: 403 GAASHDRDYSNSIVLGNNVSIPGVGLALRT--DEGKKYTMISALDALKNKSSVVDKDMYV 462

Query: 429 -ECQYPQAFDPNIVQNSIVICSFSQGFLNGTSTLTAILHTAATLRFMAFVLVANPSYGDF 488
            ECQ   +FD ++++ +++ICS+S  F+ G ST+   L  A  L     V   +P    F
Sbjct: 463 GECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGF 522

Query: 489 IAEPIPFRIPGILVPSVSDSQVILKYYEENICKDGRGM-VTNFKGKAAIGEGRFASFGGQ 548
              P P  +PGI++PS  DS+V+LKYY  ++ +DG    +  F   AAI  G+ A+F  +
Sbjct: 523 QINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNR 582

Query: 549 APTVSRFSSRGPDYMNSNRTAADVLKPDILAPGHQVWAAWSPLSASEPLLKGYHFALLSG 608
           AP +  +S+RGPD  +S    AD+LKP+++APG+ +W AWS  +      +G  FA++SG
Sbjct: 583 APKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSG 642

Query: 609 TSMAAPHIVGIAALIKQKNPSWTPSMIASAMSTTATKYDMNGELIQAE---GYGIHSLYP 668
           TSMAAPH+ G+AAL+KQK   ++PS IASA+STT+  +D  GE I A+        ++ P
Sbjct: 643 TSMAAPHVAGVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISP 702

Query: 669 STPFDLGAGLVSPTNALDPGLVFPSEYEDYINFLCSLPGVDPAVIKSTTGRHCNASLSQP 728
           +TPFD+G G V+ T ALDPGL+F + +EDY++FLC + G  P V+ + TG +C  + +  
Sbjct: 703 ATPFDMGNGFVNATAALDPGLIFDTSFEDYMSFLCGINGSAP-VVFNYTGTNCLRNNATI 762

Query: 729 HPADLNLPSLTISSLVGQQVVQRRVKNVGGKVETYVWSVIPPNGTTVDINPPWFAIAPEG 788
             +DLNLPS+T+S L   + VQR + N+ G  ETY  S+I P    ++++P  F+IA   
Sbjct: 763 SGSDLNLPSITVSKLNNTRTVQRLMTNIAGN-ETYTVSLITPFDVLINVSPTQFSIASGE 822

Query: 789 VQNLEIQINVTHKMDHFSFGEIVLTGSLNHIARIPLSV 810
            + L + +         SFG I L G+  HI RIP+SV
Sbjct: 823 TKLLSVILTAKRNSSISSFGGIKLLGNAGHIVRIPVSV 848

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4HYR61.6e-25858.07Subtilisin-like protease SBT2.4 OS=Arabidopsis thaliana OX=3702 GN=SBT2.4 PE=1 S... [more]
O644811.8e-17743.93Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S... [more]
Q9SZV51.5e-17143.37Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S... [more]
Q9FI124.5e-16843.94Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 S... [more]
Q9SUN62.3e-16443.67Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022142659.10.099.39subtilisin-like protease SBT2.4 isoform X1 [Momordica charantia][more]
XP_022142660.10.099.26subtilisin-like protease SBT2.4 isoform X2 [Momordica charantia][more]
XP_023523808.10.076.81subtilisin-like protease SBT2.4 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_038895834.10.081.36subtilisin-like protease SBT2.4 [Benincasa hispida][more]
XP_022954879.10.076.56subtilisin-like protease SBT2.4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CM390.099.39subtilisin-like protease SBT2.4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1CLJ50.099.26subtilisin-like protease SBT2.4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1GUB50.076.56subtilisin-like protease SBT2.4 OS=Cucurbita moschata OX=3662 GN=LOC111457007 PE... [more]
A0A6J1JWQ40.076.32subtilisin-like protease SBT2.4 OS=Cucurbita maxima OX=3661 GN=LOC111490380 PE=3... [more]
A0A1S3C9G90.075.43LOW QUALITY PROTEIN: subtilisin-like protease SBT2.4 OS=Cucumis melo OX=3656 GN=... [more]
Match NameE-valueIdentityDescription
AT1G62340.11.1e-25958.07PA-domain containing subtilase family protein [more]
AT2G19170.11.3e-17843.93subtilisin-like serine protease 3 [more]
AT4G30020.11.1e-17243.37PA-domain containing subtilase family protein [more]
AT5G44530.13.2e-16943.94Subtilase family protein [more]
AT4G20430.11.6e-16543.67Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 153..172
score: 38.34
coord: 232..245
score: 44.53
coord: 593..609
score: 59.77
NoneNo IPR availableGENE3D3.50.30.30coord: 366..531
e-value: 3.0E-128
score: 430.5
NoneNo IPR availableGENE3D2.60.40.2310coord: 685..812
e-value: 2.4E-14
score: 55.2
NoneNo IPR availablePANTHERPTHR10795:SF384SUBTILISIN-LIKE PROTEASE SBT2.6coord: 42..810
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 136..668
score: 28.296608
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 372..505
e-value: 8.51453E-9
score: 52.4159
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 27..123
e-value: 1.4E-13
score: 51.3
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 153..644
e-value: 3.4E-42
score: 144.6
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 715..809
e-value: 8.0E-13
score: 48.4
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 19..123
e-value: 1.4E-8
score: 37.1
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 155..681
e-value: 3.0E-128
score: 430.5
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 125..672
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 42..810
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 158..169
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 594..604
IPR022398Peptidase S8, subtilisin, His-active sitePROSITEPS00137SUBTILASE_HIScoord: 236..246
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 122..630
e-value: 2.4799E-105
score: 324.551

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0533.1MC03g0533.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process
biological_process GO:0090558 plant epidermis development
biological_process GO:0006508 proteolysis
cellular_component GO:0043229 intracellular organelle
cellular_component GO:0016020 membrane
molecular_function GO:0005488 binding
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity