MC03g0525 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0525
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein of unknown function (DUF3537)
LocationMC03: 12041780 .. 12045946 (-)
RNA-Seq ExpressionMC03g0525
SyntenyMC03g0525
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGCACCTTCAAGCAGTGGCTCAACTGCAACAAAGAAGATATACACGTGGCAACTTCAATGTGGACACTGCTCCCATTGGGCCCCACTTCTGACTTTACTTTCGTCTCACAGCGAGATATCTCATATAATCCAGAAACTCCACAGATTATTTTAATATATATAACCACACAAATAAATAAATAAATAATTAATGAATTAAATATAATAACAAAAACTTAAATATAATTATATATTTCCCTCTCTCCTCCCCTTTCCCCCACGTCATCTCTCTCTCTCTCTCCGCTCTATGAGAATCTCCCGCTTCTCTGTTCCGGCCGCGAAGCAGATTCCGAAGCTATGGAAGACGAGAAGAAGAAAGCCTCGTCTCAAATCGATTCGCAACAGCATCAATTGCTCGATCCGGAAAAATCAGAAGCAGCAGCCGAATCCGAATCGAGAAGCGCCTTCGAATCTTACCTGAAATGGATTTGCAGAATCATCGTGGATCAGTCGAATCCATGGCGCGCTTCGGTCTCCTGCTTCGTGTTCTTCGTCTTAGCAATCGCCGTCCCCCTCGCCTCGCACTTCGCTCTCGCTTGCGCCAATTGCGACGAAGATCACAGCAGGCCTTTCCATGTTGTCGTCCAGCTCTCGCTCTCCGCCGCCGCGACGCTCTCCTTCGCCTGCCTCTCTTTTTGGCTCCGTCTCTACGGATTGAGGCGGTTTCTGTTCCTCGATAAGCTCTGTGAAGCGAATCGGAAGGTTCGGGATGAGTATTCGAAGCAATTGCAGGTATGTTTTGATCCTTTTTCTTTTTCCTTTTTTTTTTTTTGGGTTCAGAGCATTTGAAAATCCCCTTTCGTGAAGATGAAGACGAAGACTAATCATTTTCGATCGTTATTTTATTTTATTTATTTAATGAGTCCATATTTTCCGAAATTATTATTATTATAATATTTTATTTTGTTAAATATGTGTTTTGCGCAAGTCGATGCCAGTTGATGGAAAAATAATAATTAAACGATGTTGATGTAAAATAAAAGAATGTCTCTAGTCCATAGAGCTTGATTAGACAAATTATTATTATATTAGCTTATGAATTTATCAAAACGAACGTATTTTTTACTATTTCAGACTTTGATTATTATTAAAAAAAAAAAGACTTTGATTATCATTTTAAATTCTAAAAATGTTTGCTGAAGGTATACATAAATAAATAAATAAATATATATATATATATATTAATTTTAATTTGAAAGTTTACAAATATAGTATTATGTCTGCCTAACACCATTTTAAAAAAGTTTTACAATATGCATATATAAATTTTAATTTTAAATCTTTCTCACTTATCTGTCCTCTTCATTTTTTAAAAAATTTAAAAGTATGCATCTAGAAAGTTAAATGTTAAAATCACACTTGTTTCAAAAAAAATAAAAATAATAAATAATAATAATTAAATCACACTTCCTCTAATTTCTAAGTTTGCTATTAATATTTTTTTTAAGTCGATCACTTTTTTTATAAGTATTTATTTTTATATGTTAAATTATAATTTTAATATATGTACCTTAATCGTAATTTAATTTATTTTCTATACTTTTAACGCTATTTTTCTAATTAATTTTTTTATAGATTCATACTTGTTGGTAATTTTTTAAAAAATAAACTCAATTTAACTAGACAGCAGAAATTTAAAAGTAAATATGAAAAATAATTGACACTTGTCTCTAGAGATCATGTCCTATCCCACTTGTTCGTATTAAAAATGGTGGAAGGTCAAAAAAATATAAATTTTTTTATAATGTCCAATTGTTAGCTTTTTAATTTTAGTACCTAACAGTTGTTAGTGCAGTAAAAAATGTATAATTTTTCCTTTAAAAGATCTAATCAGAATTAAGTGTCCATTTGGTAATTATTTAGTATTTGTAGTTTTTAAATACATTTGATTTGTTAATTACCAAAATTTTCATCTAAGAAACCACATTATATTTATAAATGATTACATTTACAAATTATCTTAAAGCTTTTGAGATTTATTGACATTATTTAACAAGTTTAGAAACTCTCATTAAATTTTAAAAATATTGTTCACTCTAGAGAACTAAATAAATCATATTACAAATTTTATTACAATGTTTAAATTGACCAATTTAAACGATATAAATAGATTACTATAAATTAAAGATCAGGATTAAATTGTTACTTCAACAGAATTAAAATTATTACTGAATTTTAGACATGAATAATGTTGTTTTGTGGCAGAGATCAATGGAGCTGATCTGCTTCTTCCTCCTCCCATGTTTCACGGCAGAAGCAGCGTACAAAGTGTGGTGGTACGTCTCAGCAGCCAACCAAATCCCATACTACGGCAACAACATGTACATCAGCTACATCACCAGCTGTGCACTGGAGCTCTGCTCCTGGCTCTACAGAACCTCCATCTTCTTCTTCGTCTGCATCCTCTTCCGCCTCGTCTGCCGCCTCCAGATGGTGTGCCTCGAAGATTTCGGGCGCGCCTTCCATCGGGAGACGGAGGTGGGCGCCATCTTGAGGCAGCATTTGGGGCTCAGAAGAACCCTCAGAATCGTCAGCCATCGGTTCAGAGTGTTCATGTTGCTGTCTCTGGTTTTGGTCACCGCCAGTCAATTTATATCTCTCTTGATGACTACCAGATCCAATGCCCATGTCAATCTCTCCAAGGCTGGACAACTTGCGGTAATCTTAATTACTTCTTCCCTCCTGGATTCACAACTTTTAATTGTCAATTTTGTAACGGTAGTTGCTTTTGATCGGTAAGCACGCTTGAGTTTTATCATTTTTTTATTCACCTTAATTGTCATTTTTCAGTACAATATGTTGGGGTGAAAATTCAAATTCATGATCTTTTAATTCGGATATAATTAATTGTCTTGACCAATTGAATTATAAGATTGGCTAATTGTCACTTTTTTTTTGGGTATAAGTAGAAGATACTTGCTTTAAACACCTATAATTAGTAATTTAGTGGGAATTTGATTTGGTTACATTGGTAAAAGCTATGAATTTTGAGAATGCAGCTATGCTCCGTCAGCCTGGTGACAGGCATCTTCATATGCCTCCGAAGTGCTGCAAAGATCACCCACAAGGCACAGTCCATCACGTGCCTTGCTGCCAAGTGGCACGTCGCCGCCATCATAAACACCTTCGACGACCTCGACGGTGAGACGCCGACGGCAGCTCGGGTCATGTCAACCATTGTATCTAATTCTGATGACGAATATGAGGATGATGACGACTTGGACGATGCCAAATTAATGCCAGTTTTTGCCCACACAATCTCATTCCAAAAGAGGCAGGCATTAGGTAAGTAAACATAGCTCTCTTCAACTTTTAATATTGTGTAAGTCCCTAAACTTTAAATCCTAAAAGAATGAAAGAAAACCATTCCAGAACAAGAAAATGACATCGCGATTAAGAAGCTTAGAATCTAAACATCAATTTTTTTGTAGCTGATTTTTTTTTTAACTGTATGCATCAAAAGGTGCCCGTAAGTAAGAAGTGTCTAATAGATCGTCAAAGTTCTCTATCTCCTAAAGGAGATGCAATATCAAAGGGCTTGGATCTTATGGTATCACCTACCATAAGGTTTTGGGTTCAAGTCCTGTAAGTTTCGATTATAGGAATTCGCAGCGTAAGCCATCCGACATTAATTCTGAAAAATTAAAAAAACAAAAAAGGGTCCAATTTCTCCTATTGAATACTTTTTAAAACATTTCAGTCCTCTTAGATACAAAGTCGAAAGTTCATAAAAGTATTATAAACTTTTTTTTTTGTGTGTGCAGTGGTATATTTGAGGAATAACAAAGCAGGAATTACAGTGTATGGATTCATGGTGGACAGAACATGGCTGAAATCCATTTTTGCCATTGAACTTGCACTTGTCCTGTGGCTGCTCAACAAGACTGTTGGTATTTCTTGAACTTTTTTGCTACCTCTTACACAATTTAAATTTCCTAATCTCCATAAATATTATTTATGAATTTTACTTATATATATACATACATATATACATACATGTAGTATATGCGTGTGTATTTATGTGGATCTACAAGAAATTTTGATTTGTACGATGAAATCTTTGCGACCTGAAATAACGTCGAAAATGTTACAATGAAATAATTAATTTCAAGTTATTGTTTCCTCTC

mRNA sequence

GTGCACCTTCAAGCAGTGGCTCAACTGCAACAAAGAAGATATACACGTGGCAACTTCAATGTGGACACTGCTCCCATTGGGCCCCACTTCTGACTTTACTTTCGTCTCACAGCGAGATATCTCATATAATCCAGAAACTCCACAGATTATTTTAATATATATAACCACACAAATAAATAAATAAATAATTAATGAATTAAATATAATAACAAAAACTTAAATATAATTATATATTTCCCTCTCTCCTCCCCTTTCCCCCACGTCATCTCTCTCTCTCTCTCCGCTCTATGAGAATCTCCCGCTTCTCTGTTCCGGCCGCGAAGCAGATTCCGAAGCTATGGAAGACGAGAAGAAGAAAGCCTCGTCTCAAATCGATTCGCAACAGCATCAATTGCTCGATCCGGAAAAATCAGAAGCAGCAGCCGAATCCGAATCGAGAAGCGCCTTCGAATCTTACCTGAAATGGATTTGCAGAATCATCGTGGATCAGTCGAATCCATGGCGCGCTTCGGTCTCCTGCTTCGTGTTCTTCGTCTTAGCAATCGCCGTCCCCCTCGCCTCGCACTTCGCTCTCGCTTGCGCCAATTGCGACGAAGATCACAGCAGGCCTTTCCATGTTGTCGTCCAGCTCTCGCTCTCCGCCGCCGCGACGCTCTCCTTCGCCTGCCTCTCTTTTTGGCTCCGTCTCTACGGATTGAGGCGGTTTCTGTTCCTCGATAAGCTCTGTGAAGCGAATCGGAAGGTTCGGGATGAGTATTCGAAGCAATTGCAGAGATCAATGGAGCTGATCTGCTTCTTCCTCCTCCCATGTTTCACGGCAGAAGCAGCGTACAAAGTGTGGTGGTACGTCTCAGCAGCCAACCAAATCCCATACTACGGCAACAACATGTACATCAGCTACATCACCAGCTGTGCACTGGAGCTCTGCTCCTGGCTCTACAGAACCTCCATCTTCTTCTTCGTCTGCATCCTCTTCCGCCTCGTCTGCCGCCTCCAGATGGTGTGCCTCGAAGATTTCGGGCGCGCCTTCCATCGGGAGACGGAGGTGGGCGCCATCTTGAGGCAGCATTTGGGGCTCAGAAGAACCCTCAGAATCGTCAGCCATCGGTTCAGAGTGTTCATGTTGCTGTCTCTGGTTTTGGTCACCGCCAGTCAATTTATATCTCTCTTGATGACTACCAGATCCAATGCCCATGTCAATCTCTCCAAGGCTGGACAACTTGCGCTATGCTCCGTCAGCCTGGTGACAGGCATCTTCATATGCCTCCGAAGTGCTGCAAAGATCACCCACAAGGCACAGTCCATCACGTGCCTTGCTGCCAAGTGGCACGTCGCCGCCATCATAAACACCTTCGACGACCTCGACGGTGAGACGCCGACGGCAGCTCGGGTCATGTCAACCATTGTATCTAATTCTGATGACGAATATGAGGATGATGACGACTTGGACGATGCCAAATTAATGCCAGTTTTTGCCCACACAATCTCATTCCAAAAGAGGCAGGCATTAGTGGTATATTTGAGGAATAACAAAGCAGGAATTACAGTGTATGGATTCATGGTGGACAGAACATGGCTGAAATCCATTTTTGCCATTGAACTTGCACTTGTCCTGTGGCTGCTCAACAAGACTGTTGGTATTTCTTGAACTTTTTTGCTACCTCTTACACAATTTAAATTTCCTAATCTCCATAAATATTATTTATGAATTTTACTTATATATATACATACATATATACATACATGTAGTATATGCGTGTGTATTTATGTGGATCTACAAGAAATTTTGATTTGTACGATGAAATCTTTGCGACCTGAAATAACGTCGAAAATGTTACAATGAAATAATTAATTTCAAGTTATTGTTTCCTCTC

Coding sequence (CDS)

ATGGAAGACGAGAAGAAGAAAGCCTCGTCTCAAATCGATTCGCAACAGCATCAATTGCTCGATCCGGAAAAATCAGAAGCAGCAGCCGAATCCGAATCGAGAAGCGCCTTCGAATCTTACCTGAAATGGATTTGCAGAATCATCGTGGATCAGTCGAATCCATGGCGCGCTTCGGTCTCCTGCTTCGTGTTCTTCGTCTTAGCAATCGCCGTCCCCCTCGCCTCGCACTTCGCTCTCGCTTGCGCCAATTGCGACGAAGATCACAGCAGGCCTTTCCATGTTGTCGTCCAGCTCTCGCTCTCCGCCGCCGCGACGCTCTCCTTCGCCTGCCTCTCTTTTTGGCTCCGTCTCTACGGATTGAGGCGGTTTCTGTTCCTCGATAAGCTCTGTGAAGCGAATCGGAAGGTTCGGGATGAGTATTCGAAGCAATTGCAGAGATCAATGGAGCTGATCTGCTTCTTCCTCCTCCCATGTTTCACGGCAGAAGCAGCGTACAAAGTGTGGTGGTACGTCTCAGCAGCCAACCAAATCCCATACTACGGCAACAACATGTACATCAGCTACATCACCAGCTGTGCACTGGAGCTCTGCTCCTGGCTCTACAGAACCTCCATCTTCTTCTTCGTCTGCATCCTCTTCCGCCTCGTCTGCCGCCTCCAGATGGTGTGCCTCGAAGATTTCGGGCGCGCCTTCCATCGGGAGACGGAGGTGGGCGCCATCTTGAGGCAGCATTTGGGGCTCAGAAGAACCCTCAGAATCGTCAGCCATCGGTTCAGAGTGTTCATGTTGCTGTCTCTGGTTTTGGTCACCGCCAGTCAATTTATATCTCTCTTGATGACTACCAGATCCAATGCCCATGTCAATCTCTCCAAGGCTGGACAACTTGCGCTATGCTCCGTCAGCCTGGTGACAGGCATCTTCATATGCCTCCGAAGTGCTGCAAAGATCACCCACAAGGCACAGTCCATCACGTGCCTTGCTGCCAAGTGGCACGTCGCCGCCATCATAAACACCTTCGACGACCTCGACGGTGAGACGCCGACGGCAGCTCGGGTCATGTCAACCATTGTATCTAATTCTGATGACGAATATGAGGATGATGACGACTTGGACGATGCCAAATTAATGCCAGTTTTTGCCCACACAATCTCATTCCAAAAGAGGCAGGCATTAGTGGTATATTTGAGGAATAACAAAGCAGGAATTACAGTGTATGGATTCATGGTGGACAGAACATGGCTGAAATCCATTTTTGCCATTGAACTTGCACTTGTCCTGTGGCTGCTCAACAAGACTGTTGGTATTTCTTGA

Protein sequence

MEDEKKKASSQIDSQQHQLLDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILRQHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARVMSTIVSNSDDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIELALVLWLLNKTVGIS
Homology
BLAST of MC03g0525 vs. NCBI nr
Match: XP_008458982.1 (PREDICTED: uncharacterized protein LOC103498231 [Cucumis melo])

HSP 1 Score: 652 bits (1683), Expect = 1.38e-232
Identity = 338/435 (77.70%), Postives = 375/435 (86.21%), Query Frame = 0

Query: 3   DEKKKASSQIDSQQHQLLDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVSCF 62
           +E+KK+  Q+DSQ+ +  + E SE+  E+     FES+LKWIC  I+D SN +RAS+SC 
Sbjct: 4   EEEKKSQPQMDSQKSKSAESE-SESELEAAELRRFESFLKWIC--IMDHSNLYRASLSCI 63

Query: 63  VFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRR 122
           VFFV  IAVP+ASHFAL+C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLRL+GL R
Sbjct: 64  VFFVFGIAVPIASHFALSCSDCDEDHQRPFHVVVQLSLSAVATLSFLCLSLWLRLFGLNR 123

Query: 123 FLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGN 182
           FLFLDKL EA+ K+R EY +QLQRSMEL+ FFLLPCF AEA YK+WWY+SAA +IPYY N
Sbjct: 124 FLFLDKLSEASPKIRAEYFRQLQRSMELMSFFLLPCFMAEAGYKIWWYISAAKEIPYYTN 183

Query: 183 NMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILR 242
           NMYISYITSC LELCSWLYRTSIFFFVCILFRL+C LQM+ LEDF   F  ETEVG IL 
Sbjct: 184 NMYISYITSCTLELCSWLYRTSIFFFVCILFRLICCLQMIRLEDFASIFRSETEVGTILI 243

Query: 243 QHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSL 302
           QHLGLRRT  I+SHRFRVFMLLSL+LVTASQFISLLMTTRS AHVNLSKAGQLALCS+SL
Sbjct: 244 QHLGLRRTFTIISHRFRVFMLLSLILVTASQFISLLMTTRSKAHVNLSKAGQLALCSISL 303

Query: 303 VTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARVMSTIV-SNSD 362
           VTG+FICLRSAAKITHKAQSITCLAAKWHV+A+INTFDDLD ETP  A ++  IV SNSD
Sbjct: 304 VTGLFICLRSAAKITHKAQSITCLAAKWHVSAVINTFDDLDTETPPTASLVPNIVESNSD 363

Query: 363 DEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIE 422
           DE  D+DDLDD KLMPVFAHTISFQKRQALV YLRNNKAGITVYGFMVDRTWLKSIFAIE
Sbjct: 364 DEDGDEDDLDDPKLMPVFAHTISFQKRQALVTYLRNNKAGITVYGFMVDRTWLKSIFAIE 423

Query: 423 LALVLWLLNKTVGIS 436
           LAL LWLLNKTVG+S
Sbjct: 424 LALFLWLLNKTVGVS 435

BLAST of MC03g0525 vs. NCBI nr
Match: XP_038893800.1 (uncharacterized protein LOC120082620 [Benincasa hispida])

HSP 1 Score: 647 bits (1668), Expect = 2.36e-230
Identity = 333/436 (76.38%), Postives = 370/436 (84.86%), Query Frame = 0

Query: 1   MEDEKKKASSQIDSQQHQLLDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVS 60
           ME E+KK+  QIDSQ+  L    +SE  +E+     F+S LKWIC  I D SNP+RAS+S
Sbjct: 1   METEEKKSQPQIDSQK--LESEPESEPESEAAELRRFKSILKWIC--ITDHSNPYRASLS 60

Query: 61  CFVFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGL 120
           C VFFV AIAVP+ASHFAL+C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLR +GL
Sbjct: 61  CIVFFVFAIAVPVASHFALSCSDCDEDHQRPFHVVVQLSLSAVATLSFLCLSLWLRFFGL 120

Query: 121 RRFLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYY 180
            RFLFLDKL EA+ +VR EY +QLQRSM L+ FFLLPCF AEA YK+WWY+SAA +IPYY
Sbjct: 121 NRFLFLDKLSEASPRVRAEYFRQLQRSMVLMSFFLLPCFMAEAGYKIWWYISAAKEIPYY 180

Query: 181 GNNMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAI 240
            NN+YISYI SC LELCSWLYRTSIFFFVCILFRL+C LQM+ LEDF   FHRE EVG I
Sbjct: 181 TNNIYISYIISCTLELCSWLYRTSIFFFVCILFRLICHLQMIRLEDFASIFHREAEVGTI 240

Query: 241 LRQHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSV 300
           L QHL LRRT  I+SHRFR F+LLSL+LVTASQFISLLMTTRS AHVNLSKAGQLALCS+
Sbjct: 241 LMQHLVLRRTFTIISHRFRAFILLSLILVTASQFISLLMTTRSKAHVNLSKAGQLALCSI 300

Query: 301 SLVTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARVMSTIVSNS 360
           SLVTG+FICLRSAAKITHKAQSITCLAAKWHV+A+INTFDDLD ETP  A ++S IV ++
Sbjct: 301 SLVTGLFICLRSAAKITHKAQSITCLAAKWHVSAVINTFDDLDSETPPTASLISNIVESN 360

Query: 361 DDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAI 420
            DE  D+DDLDDAKLMPVFAHTISFQKRQALV YLRNNKAGITVYGF VDRTWLKSIFAI
Sbjct: 361 SDEESDEDDLDDAKLMPVFAHTISFQKRQALVTYLRNNKAGITVYGFEVDRTWLKSIFAI 420

Query: 421 ELALVLWLLNKTVGIS 436
           ELAL LWLLNKTVG+S
Sbjct: 421 ELALFLWLLNKTVGVS 432

BLAST of MC03g0525 vs. NCBI nr
Match: XP_011660297.1 (uncharacterized protein LOC101203162 [Cucumis sativus])

HSP 1 Score: 638 bits (1645), Expect = 8.03e-227
Identity = 329/439 (74.94%), Postives = 374/439 (85.19%), Query Frame = 0

Query: 1   MEDEKKKASSQIDSQQHQLLDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVS 60
           ME  +KK+  QIDSQ+ +  + E   +  E+      ES+L+WIC  I+D SN +RASVS
Sbjct: 1   METVEKKSQPQIDSQKSKSAELE---SELEAPELRKLESFLRWIC--IMDHSNLYRASVS 60

Query: 61  CFVFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGL 120
           C +FFV  IAVP+ASHF L+C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLR++GL
Sbjct: 61  CIMFFVFGIAVPIASHFGLSCSDCDEDHQRPFHVVVQLSLSAVATLSFLCLSLWLRVFGL 120

Query: 121 RRFLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYY 180
            RFLFLDKLCEA+ K+R EY +QLQ+SM+L+ FFLLPCF AEA YK+WWY+SAA +IPYY
Sbjct: 121 NRFLFLDKLCEASPKIRAEYFRQLQKSMKLMSFFLLPCFMAEAGYKIWWYISAAKEIPYY 180

Query: 181 GNNMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAI 240
            NNMYISY+TSC LELCSWLYRTSIFFFVCI FRL+C LQM+ LEDF  +F  ETEVG I
Sbjct: 181 TNNMYISYVTSCTLELCSWLYRTSIFFFVCIFFRLICCLQMIRLEDFASSFRSETEVGTI 240

Query: 241 LRQHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSV 300
           L QHLGLRRT  ++SHRFRVFMLLSL+LVTASQFISLLMTTRS AH NLSK+GQLALCS+
Sbjct: 241 LIQHLGLRRTFTVISHRFRVFMLLSLILVTASQFISLLMTTRSKAHANLSKSGQLALCSI 300

Query: 301 SLVTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGE-TPTAARVMSTIVSN 360
           SLVTG+FICLRSAAKITHKAQSITCLAAKWHV+A+INTFD+LD E TPTA+ V + + SN
Sbjct: 301 SLVTGLFICLRSAAKITHKAQSITCLAAKWHVSAVINTFDELDTEMTPTASFVPNVVESN 360

Query: 361 SDDEY--EDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSI 420
           SDDE   ED+DDLDDAKLMPVFAHTISFQKRQALV YLRNNKAGITVYGFMVDRTWLKSI
Sbjct: 361 SDDEDGDEDEDDLDDAKLMPVFAHTISFQKRQALVTYLRNNKAGITVYGFMVDRTWLKSI 420

Query: 421 FAIELALVLWLLNKTVGIS 436
           FAIELAL LWLLNKTVG+S
Sbjct: 421 FAIELALFLWLLNKTVGVS 434

BLAST of MC03g0525 vs. NCBI nr
Match: XP_022954540.1 (uncharacterized protein LOC111456780 [Cucurbita moschata])

HSP 1 Score: 637 bits (1644), Expect = 1.47e-226
Identity = 333/444 (75.00%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   MEDEKKKASSQIDSQQHQLLDPEKSEAAAESESRSA--------FESYLKWICRIIVDQS 60
           MED +KK+ S   S     ++  KSE   ESES S         FES+LKWIC  I+D S
Sbjct: 1   MEDPEKKSPSPSPSPSPSQINSRKSELKLESESESESDSAELRRFESFLKWIC--IMDHS 60

Query: 61  NPWRASVSCFVFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLS 120
           NP+ A++SCF+F   AIAVP+ASHFAL+C++CDEDH RPFHVVVQLSLSA ATLSF CLS
Sbjct: 61  NPFTATLSCFLFSAFAIAVPIASHFALSCSDCDEDHRRPFHVVVQLSLSAVATLSFGCLS 120

Query: 121 FWLRLYGLRRFLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVS 180
            WLR  GL RFLFLDKLCE++ K RDEYSKQL+RSMELI FFLLPCF AEAAYK+WWYVS
Sbjct: 121 TWLRHLGLSRFLFLDKLCESSHKARDEYSKQLKRSMELISFFLLPCFMAEAAYKIWWYVS 180

Query: 181 AANQIPYYGNNMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFH 240
           AAN+IPYYGN MY+SYITSC LEL SWLYRTSIFFFVCILFRLVCRLQM+ LEDF   FH
Sbjct: 181 AANEIPYYGN-MYLSYITSCTLELFSWLYRTSIFFFVCILFRLVCRLQMIRLEDFVSVFH 240

Query: 241 RETEVGAILRQHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKA 300
           RE++VG IL QHLGLRRTL  +SHRFRVFM LSL+LVTASQFISLLMTTRS+A  NLSK 
Sbjct: 241 RESDVGTILMQHLGLRRTLTTISHRFRVFMFLSLILVTASQFISLLMTTRSHALANLSKT 300

Query: 301 GQLALCSVSLVTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARV 360
           GQLALCS+SLVTG+FICLRSAAKITHKAQSITCLAAKWHV+A INTFDDLD ETPTA+ +
Sbjct: 301 GQLALCSISLVTGLFICLRSAAKITHKAQSITCLAAKWHVSAAINTFDDLDNETPTASVI 360

Query: 361 MSTIVSNSDDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRT 420
            +   ++ DDE +D+DD DD KLMPVFAHTISFQKRQALV YL+NNK GITVYGF+VDRT
Sbjct: 361 AAFEFNSDDDEEDDEDDEDDMKLMPVFAHTISFQKRQALVTYLKNNKGGITVYGFVVDRT 420

Query: 421 WLKSIFAIELALVLWLLNKTVGIS 436
           WLKS+FAIELAL+LWLLNKTVGIS
Sbjct: 421 WLKSVFAIELALMLWLLNKTVGIS 441

BLAST of MC03g0525 vs. NCBI nr
Match: KAG6573486.1 (hypothetical protein SDJN03_27373, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 637 bits (1642), Expect = 2.97e-226
Identity = 328/417 (78.66%), Postives = 363/417 (87.05%), Query Frame = 0

Query: 24  KSEAAAESESRSA----FESYLKWICRIIVDQSNPWRASVSCFVFFVLAIAVPLASHFAL 83
           KSE   ESES SA    FES+LKWIC  I+D SNP+ A++SCF+F   AIAVP+ASHFAL
Sbjct: 28  KSELKLESESDSAELRRFESFLKWIC--IMDHSNPFTATLSCFLFSAFAIAVPIASHFAL 87

Query: 84  ACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLDKLCEANRKVRDE 143
           +C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLR  GL RFLFLDKLCE++ K RDE
Sbjct: 88  SCSDCDEDHRRPFHVVVQLSLSAVATLSFGCLSAWLRHLGLSRFLFLDKLCESSHKARDE 147

Query: 144 YSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYISYITSCALELCSW 203
           YSKQL+RSMELI FFLLPCF AEAAYK+WWYVSAAN+IPYYGN MY+SYITSC LEL SW
Sbjct: 148 YSKQLKRSMELISFFLLPCFMAEAAYKIWWYVSAANEIPYYGN-MYLSYITSCTLELFSW 207

Query: 204 LYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILRQHLGLRRTLRIVSHRFR 263
           LYRTSIFFFVCILFRLVCRLQM+ LEDF   FHRE++VG IL QHLGLRRTL ++SHRFR
Sbjct: 208 LYRTSIFFFVCILFRLVCRLQMIRLEDFVSVFHRESDVGTILMQHLGLRRTLTLISHRFR 267

Query: 264 VFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGIFICLRSAAKITHK 323
           VFM LSL+LVTASQFISLLMTTRS+A  NLSK GQLALCS+SLVTG+FICLRSAAKITHK
Sbjct: 268 VFMFLSLILVTASQFISLLMTTRSHALANLSKTGQLALCSISLVTGLFICLRSAAKITHK 327

Query: 324 AQSITCLAAKWHVAAIINTFDDLDGETPTAARVMSTIVSNSDDEYEDDDDLDDAKLMPVF 383
           AQSITCLAAKWHV+A INTFDDLD ETPTA+ + +   ++ DDE ED+DD DD KLMPVF
Sbjct: 328 AQSITCLAAKWHVSAAINTFDDLDNETPTASVIAAFEFNSDDDEEEDEDDEDDMKLMPVF 387

Query: 384 AHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIELALVLWLLNKTVGIS 436
           AHTISFQKRQALV YL+NNK GITVYGF+VDRTWLKS+FAIELAL LWLLNKTVGIS
Sbjct: 388 AHTISFQKRQALVTYLKNNKGGITVYGFVVDRTWLKSVFAIELALTLWLLNKTVGIS 441

BLAST of MC03g0525 vs. ExPASy TrEMBL
Match: A0A1S3C949 (uncharacterized protein LOC103498231 OS=Cucumis melo OX=3656 GN=LOC103498231 PE=4 SV=1)

HSP 1 Score: 652 bits (1683), Expect = 6.66e-233
Identity = 338/435 (77.70%), Postives = 375/435 (86.21%), Query Frame = 0

Query: 3   DEKKKASSQIDSQQHQLLDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVSCF 62
           +E+KK+  Q+DSQ+ +  + E SE+  E+     FES+LKWIC  I+D SN +RAS+SC 
Sbjct: 4   EEEKKSQPQMDSQKSKSAESE-SESELEAAELRRFESFLKWIC--IMDHSNLYRASLSCI 63

Query: 63  VFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRR 122
           VFFV  IAVP+ASHFAL+C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLRL+GL R
Sbjct: 64  VFFVFGIAVPIASHFALSCSDCDEDHQRPFHVVVQLSLSAVATLSFLCLSLWLRLFGLNR 123

Query: 123 FLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGN 182
           FLFLDKL EA+ K+R EY +QLQRSMEL+ FFLLPCF AEA YK+WWY+SAA +IPYY N
Sbjct: 124 FLFLDKLSEASPKIRAEYFRQLQRSMELMSFFLLPCFMAEAGYKIWWYISAAKEIPYYTN 183

Query: 183 NMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILR 242
           NMYISYITSC LELCSWLYRTSIFFFVCILFRL+C LQM+ LEDF   F  ETEVG IL 
Sbjct: 184 NMYISYITSCTLELCSWLYRTSIFFFVCILFRLICCLQMIRLEDFASIFRSETEVGTILI 243

Query: 243 QHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSL 302
           QHLGLRRT  I+SHRFRVFMLLSL+LVTASQFISLLMTTRS AHVNLSKAGQLALCS+SL
Sbjct: 244 QHLGLRRTFTIISHRFRVFMLLSLILVTASQFISLLMTTRSKAHVNLSKAGQLALCSISL 303

Query: 303 VTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARVMSTIV-SNSD 362
           VTG+FICLRSAAKITHKAQSITCLAAKWHV+A+INTFDDLD ETP  A ++  IV SNSD
Sbjct: 304 VTGLFICLRSAAKITHKAQSITCLAAKWHVSAVINTFDDLDTETPPTASLVPNIVESNSD 363

Query: 363 DEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIE 422
           DE  D+DDLDD KLMPVFAHTISFQKRQALV YLRNNKAGITVYGFMVDRTWLKSIFAIE
Sbjct: 364 DEDGDEDDLDDPKLMPVFAHTISFQKRQALVTYLRNNKAGITVYGFMVDRTWLKSIFAIE 423

Query: 423 LALVLWLLNKTVGIS 436
           LAL LWLLNKTVG+S
Sbjct: 424 LALFLWLLNKTVGVS 435

BLAST of MC03g0525 vs. ExPASy TrEMBL
Match: A0A0A0M3M6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G699550 PE=4 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 3.89e-227
Identity = 329/439 (74.94%), Postives = 374/439 (85.19%), Query Frame = 0

Query: 1   MEDEKKKASSQIDSQQHQLLDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVS 60
           ME  +KK+  QIDSQ+ +  + E   +  E+      ES+L+WIC  I+D SN +RASVS
Sbjct: 1   METVEKKSQPQIDSQKSKSAELE---SELEAPELRKLESFLRWIC--IMDHSNLYRASVS 60

Query: 61  CFVFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGL 120
           C +FFV  IAVP+ASHF L+C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLR++GL
Sbjct: 61  CIMFFVFGIAVPIASHFGLSCSDCDEDHQRPFHVVVQLSLSAVATLSFLCLSLWLRVFGL 120

Query: 121 RRFLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYY 180
            RFLFLDKLCEA+ K+R EY +QLQ+SM+L+ FFLLPCF AEA YK+WWY+SAA +IPYY
Sbjct: 121 NRFLFLDKLCEASPKIRAEYFRQLQKSMKLMSFFLLPCFMAEAGYKIWWYISAAKEIPYY 180

Query: 181 GNNMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAI 240
            NNMYISY+TSC LELCSWLYRTSIFFFVCI FRL+C LQM+ LEDF  +F  ETEVG I
Sbjct: 181 TNNMYISYVTSCTLELCSWLYRTSIFFFVCIFFRLICCLQMIRLEDFASSFRSETEVGTI 240

Query: 241 LRQHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSV 300
           L QHLGLRRT  ++SHRFRVFMLLSL+LVTASQFISLLMTTRS AH NLSK+GQLALCS+
Sbjct: 241 LIQHLGLRRTFTVISHRFRVFMLLSLILVTASQFISLLMTTRSKAHANLSKSGQLALCSI 300

Query: 301 SLVTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGE-TPTAARVMSTIVSN 360
           SLVTG+FICLRSAAKITHKAQSITCLAAKWHV+A+INTFD+LD E TPTA+ V + + SN
Sbjct: 301 SLVTGLFICLRSAAKITHKAQSITCLAAKWHVSAVINTFDELDTEMTPTASFVPNVVESN 360

Query: 361 SDDEY--EDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSI 420
           SDDE   ED+DDLDDAKLMPVFAHTISFQKRQALV YLRNNKAGITVYGFMVDRTWLKSI
Sbjct: 361 SDDEDGDEDEDDLDDAKLMPVFAHTISFQKRQALVTYLRNNKAGITVYGFMVDRTWLKSI 420

Query: 421 FAIELALVLWLLNKTVGIS 436
           FAIELAL LWLLNKTVG+S
Sbjct: 421 FAIELALFLWLLNKTVGVS 434

BLAST of MC03g0525 vs. ExPASy TrEMBL
Match: A0A6J1GSP8 (uncharacterized protein LOC111456780 OS=Cucurbita moschata OX=3662 GN=LOC111456780 PE=4 SV=1)

HSP 1 Score: 637 bits (1644), Expect = 7.13e-227
Identity = 333/444 (75.00%), Postives = 373/444 (84.01%), Query Frame = 0

Query: 1   MEDEKKKASSQIDSQQHQLLDPEKSEAAAESESRSA--------FESYLKWICRIIVDQS 60
           MED +KK+ S   S     ++  KSE   ESES S         FES+LKWIC  I+D S
Sbjct: 1   MEDPEKKSPSPSPSPSPSQINSRKSELKLESESESESDSAELRRFESFLKWIC--IMDHS 60

Query: 61  NPWRASVSCFVFFVLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLS 120
           NP+ A++SCF+F   AIAVP+ASHFAL+C++CDEDH RPFHVVVQLSLSA ATLSF CLS
Sbjct: 61  NPFTATLSCFLFSAFAIAVPIASHFALSCSDCDEDHRRPFHVVVQLSLSAVATLSFGCLS 120

Query: 121 FWLRLYGLRRFLFLDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVS 180
            WLR  GL RFLFLDKLCE++ K RDEYSKQL+RSMELI FFLLPCF AEAAYK+WWYVS
Sbjct: 121 TWLRHLGLSRFLFLDKLCESSHKARDEYSKQLKRSMELISFFLLPCFMAEAAYKIWWYVS 180

Query: 181 AANQIPYYGNNMYISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFH 240
           AAN+IPYYGN MY+SYITSC LEL SWLYRTSIFFFVCILFRLVCRLQM+ LEDF   FH
Sbjct: 181 AANEIPYYGN-MYLSYITSCTLELFSWLYRTSIFFFVCILFRLVCRLQMIRLEDFVSVFH 240

Query: 241 RETEVGAILRQHLGLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKA 300
           RE++VG IL QHLGLRRTL  +SHRFRVFM LSL+LVTASQFISLLMTTRS+A  NLSK 
Sbjct: 241 RESDVGTILMQHLGLRRTLTTISHRFRVFMFLSLILVTASQFISLLMTTRSHALANLSKT 300

Query: 301 GQLALCSVSLVTGIFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARV 360
           GQLALCS+SLVTG+FICLRSAAKITHKAQSITCLAAKWHV+A INTFDDLD ETPTA+ +
Sbjct: 301 GQLALCSISLVTGLFICLRSAAKITHKAQSITCLAAKWHVSAAINTFDDLDNETPTASVI 360

Query: 361 MSTIVSNSDDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRT 420
            +   ++ DDE +D+DD DD KLMPVFAHTISFQKRQALV YL+NNK GITVYGF+VDRT
Sbjct: 361 AAFEFNSDDDEEDDEDDEDDMKLMPVFAHTISFQKRQALVTYLKNNKGGITVYGFVVDRT 420

Query: 421 WLKSIFAIELALVLWLLNKTVGIS 436
           WLKS+FAIELAL+LWLLNKTVGIS
Sbjct: 421 WLKSVFAIELALMLWLLNKTVGIS 441

BLAST of MC03g0525 vs. ExPASy TrEMBL
Match: A0A6J1K314 (uncharacterized protein LOC111490209 OS=Cucurbita maxima OX=3661 GN=LOC111490209 PE=4 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 1.66e-226
Identity = 332/431 (77.03%), Postives = 374/431 (86.77%), Query Frame = 0

Query: 10  SQIDSQQHQLLDPEKSEAAAESESRSA----FESYLKWICRIIVDQSNPWRASVSCFVFF 69
           SQI+S++ +L    KSE+ +ESES SA    FES+LKWIC  I+D SNP+ A++SCF+F 
Sbjct: 22  SQINSRKSEL----KSESESESESDSAELRRFESFLKWIC--IMDHSNPFTATLSCFLFS 81

Query: 70  VLAIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLF 129
             AIAVP+ASHFAL+C++CDEDH RPFHVVVQLSLSA ATLSF CLS WLR +GL RFLF
Sbjct: 82  AFAIAVPIASHFALSCSDCDEDHRRPFHVVVQLSLSAVATLSFGCLSAWLRHFGLSRFLF 141

Query: 130 LDKLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMY 189
           LDKLCE++ K RDEYSKQL+RSMELI FFLLPCF AEAAYK+WWYVSAA +IPYYGN MY
Sbjct: 142 LDKLCESSHKARDEYSKQLKRSMELISFFLLPCFMAEAAYKIWWYVSAAYEIPYYGN-MY 201

Query: 190 ISYITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILRQHL 249
           +SYITSC LEL SWLYRTSIFFFVCILFRLVCRLQM+ LEDF   FHRE++VG IL QHL
Sbjct: 202 LSYITSCTLELFSWLYRTSIFFFVCILFRLVCRLQMIRLEDFVSVFHRESDVGTILMQHL 261

Query: 250 GLRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTG 309
           GLRRTL I+SHRFRVFM LSL+LVTASQFI LLMTTRS+A  NLSK GQLALCS+SLVTG
Sbjct: 262 GLRRTLTIISHRFRVFMFLSLILVTASQFIFLLMTTRSHALANLSKTGQLALCSISLVTG 321

Query: 310 IFICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARVMSTIVSNSDDEYE 369
           +FICLRSAAKI+HKAQSITCLAAKWHV+A INTFDDLD ETPT + + +   ++ DDE +
Sbjct: 322 LFICLRSAAKISHKAQSITCLAAKWHVSAAINTFDDLDNETPTTSVIATFEFNSDDDEDD 381

Query: 370 DDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIELALV 429
           D+DD DD KLMPVFAHTISFQKRQALV YLRNNKAGITVYGF+VDRTWLKS+FAIELALV
Sbjct: 382 DEDDEDDMKLMPVFAHTISFQKRQALVTYLRNNKAGITVYGFVVDRTWLKSVFAIELALV 441

Query: 430 LWLLNKTVGIS 436
           LWLLNKTVGIS
Sbjct: 442 LWLLNKTVGIS 445

BLAST of MC03g0525 vs. ExPASy TrEMBL
Match: A0A6J1EG16 (uncharacterized protein LOC111433990 OS=Cucurbita moschata OX=3662 GN=LOC111433990 PE=4 SV=1)

HSP 1 Score: 623 bits (1607), Expect = 1.69e-221
Identity = 320/420 (76.19%), Postives = 362/420 (86.19%), Query Frame = 0

Query: 20  LDPEKSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVLAIAVPLASHFAL 79
           +D ++SE+  E+      ES+LKWIC  I DQSNP+RAS+SC +FF+ AIAVPLASHFAL
Sbjct: 12  MDSQESESELEAPELRRLESFLKWIC--INDQSNPYRASLSCLIFFIFAIAVPLASHFAL 71

Query: 80  ACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLDKLCEANRKVRDE 139
           +C++CDEDH RPFHVVVQLSLSA A LSF  LS WLRL+G  RFLFLDKL +A+ +VR E
Sbjct: 72  SCSDCDEDHQRPFHVVVQLSLSAVAMLSFLSLSLWLRLFGFNRFLFLDKLSKASPRVRAE 131

Query: 140 YSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYISYITSCALELCSW 199
           YS+QLQRS ELIC F++PCF AEAAYK+WWY++AA QIPYY NNMY+SYITSC LELCSW
Sbjct: 132 YSRQLQRSKELICLFVVPCFMAEAAYKMWWYITAAKQIPYYTNNMYLSYITSCTLELCSW 191

Query: 200 LYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILRQHLGLRRTLRIVSHRFR 259
           LYRTSIFFFVC+LFRL+C LQM+ LEDF   FHRET+VG IL  HLGLRRT  I+SHRFR
Sbjct: 192 LYRTSIFFFVCVLFRLICCLQMIRLEDFASVFHRETDVGTILVLHLGLRRTFTIISHRFR 251

Query: 260 VFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGIFICLRSAAKITHK 319
            F+LLSL+LVTASQFISLLMTT + AHVNLSKAGQLALCS+SLVTG+FICLRSAAKITHK
Sbjct: 252 AFILLSLILVTASQFISLLMTTTAKAHVNLSKAGQLALCSISLVTGLFICLRSAAKITHK 311

Query: 320 AQSITCLAAKWHVAAIINTFDDLDGET-PTAARVMSTIVSNSDDEYED--DDDLDDAKLM 379
           AQSITCLAAKWH++A+++TFDDLD +T PTAA     I SNSDDE  D  +DDLDDAKLM
Sbjct: 312 AQSITCLAAKWHISAVVSTFDDLDNKTTPTAA----IIESNSDDEDGDGDEDDLDDAKLM 371

Query: 380 PVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIELALVLWLLNKTVGIS 436
           PVFA TISFQKRQALV+YLRNNKAGITVYGFMVDRTWLKSIFAIELAL LWLLNKTVGIS
Sbjct: 372 PVFADTISFQKRQALVIYLRNNKAGITVYGFMVDRTWLKSIFAIELALFLWLLNKTVGIS 425

BLAST of MC03g0525 vs. TAIR 10
Match: AT4G22270.1 (Protein of unknown function (DUF3537) )

HSP 1 Score: 442.2 bits (1136), Expect = 4.9e-124
Identity = 236/434 (54.38%), Postives = 311/434 (71.66%), Query Frame = 0

Query: 14  SQQHQLLDPEKSEAAAESE------SRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVL 73
           S +H L +  +  A    +      SR  F S + W      DQSN   A +S  VFF+L
Sbjct: 6   SSEHLLPETTRPSAIINKQQPEFPASRFTFMSLVLWF-----DQSNFGTALLSWSVFFLL 65

Query: 74  AIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLD 133
            + VPL SHF L C++CD  H RP+ V+VQLSLS  A +SF  LS W R +G+RRFLFLD
Sbjct: 66  VVIVPLISHFLLVCSDCDFHHRRPYDVIVQLSLSIFAGISFVSLSIWSRKFGMRRFLFLD 125

Query: 134 KLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYIS 193
           KL + + KVR EY  ++QRS++ +  F+LP  T EA Y++WWY+S  NQIPY  N + +S
Sbjct: 126 KLWDVSDKVRIEYEAEIQRSLKRLMIFVLPSLTLEATYRIWWYISGFNQIPYIINPI-LS 185

Query: 194 YITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRE-TEVGAILRQHLG 253
           ++ +C L+L SWLYR S+F  VCIL+++ C LQ + L+DF R F  E T+V + L +H  
Sbjct: 186 HVVACTLQLSSWLYRNSLFIIVCILYKITCHLQTLRLDDFARCFASEITDVRSALGEHQK 245

Query: 254 LRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGI 313
           +RR LRIVSHRFR F+LLSL+LVTA+QF++LL TTR++  VN+ + G+LALCS+SLVTG+
Sbjct: 246 IRRNLRIVSHRFRRFILLSLILVTATQFMALLTTTRASVAVNIYEVGELALCSLSLVTGV 305

Query: 314 FICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDLDGETPTAARVMS-------TIVSN 373
           FICLRSA KITHKAQS+T LAAKW+V A +++FD LDGETPT + + S        I ++
Sbjct: 306 FICLRSATKITHKAQSVTSLAAKWNVCATVDSFDHLDGETPTGSIIESQVSLRGNAIETS 365

Query: 374 SDDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFA 433
            D+E E DDDLD+ K+ P++A+TIS+QKRQALV YL NNKAGITVYGF+VDR+WL +IF 
Sbjct: 366 DDEEGEGDDDLDNTKIHPIYANTISYQKRQALVTYLENNKAGITVYGFLVDRSWLNTIFG 425

BLAST of MC03g0525 vs. TAIR 10
Match: AT3G20300.1 (Protein of unknown function (DUF3537) )

HSP 1 Score: 438.7 bits (1127), Expect = 5.4e-123
Identity = 227/424 (53.54%), Postives = 312/424 (73.58%), Query Frame = 0

Query: 24  KSEAAAESESRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVLAIAVPLASHFALACAN 83
           +S + A+ E  S F  YL+W+C   VDQS+PW A +S  +F V  + VP  SHF LAC++
Sbjct: 34  RSVSHAQDELHS-FRKYLRWMC---VDQSSPWTAVLSWSMFVVFTLVVPATSHFMLACSD 93

Query: 84  CDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLDKLCEANRKVRDEYSKQ 143
           CD  HSRP+  VVQLSLS+ A LSF CLS ++  YGLRRFLF DKL + +  VR  Y+ Q
Sbjct: 94  CDSHHSRPYDSVVQLSLSSFAALSFLCLSRFVSKYGLRRFLFFDKLWDESETVRLGYTNQ 153

Query: 144 LQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYISYITSCALELCSWLYRT 203
           L RS++++ +F+ PCF A ++YK+WWY S A+QIP+ G N+ +S   +C +ELCSWLYRT
Sbjct: 154 LNRSLKILSYFVSPCFLAMSSYKIWWYASGASQIPFLG-NVILSDTVACLMELCSWLYRT 213

Query: 204 SIFFFVCILFRLVCRLQMVCLEDFGRAFHRETEVGAILRQHLGLRRTLRIVSHRFRVFML 263
           ++ F VC+LFRL+C LQ++ L+DF + F  +++VG+IL +HL +RR LRI+SHR+R F+L
Sbjct: 214 TVIFLVCVLFRLICHLQILRLQDFAQVFQMDSDVGSILSEHLRIRRHLRIISHRYRTFIL 273

Query: 264 LSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGIFICLRSAAKITHKAQSI 323
           LSL+LVT SQF SLL+TT++ A +N+ +AG+LALCS++LVT + I LRSA+KITHKAQ++
Sbjct: 274 LSLILVTGSQFYSLLITTKAYAELNIYRAGELALCSMTLVTALLILLRSASKITHKAQAV 333

Query: 324 TCLAAKWHVAAIINTFDDLDGETPTAARVMS----------TIVSNSDDEYEDDDDLDDA 383
           TCLAAKWHV A I +F+ +DGETP      S             S+S+D  +++DD D+ 
Sbjct: 334 TCLAAKWHVCATIESFETVDGETPRLVDRASGHGYYPTDDDNGESDSEDYGDEEDDFDNN 393

Query: 384 KLMPVFAH-TISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIELALVLWLLNKT 437
            L+P +A+ TISFQKRQALV Y  NN++GITV+GF +DR+ L +IF IE++LVLWLL KT
Sbjct: 394 NLIPAYAYSTISFQKRQALVNYFENNRSGITVFGFTLDRSTLHTIFGIEMSLVLWLLGKT 452

BLAST of MC03g0525 vs. TAIR 10
Match: AT1G50630.1 (Protein of unknown function (DUF3537) )

HSP 1 Score: 426.8 bits (1096), Expect = 2.1e-119
Identity = 222/425 (52.24%), Postives = 306/425 (72.00%), Query Frame = 0

Query: 30  ESESRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVLAIAVPLASHFALACANCDEDHS 89
           + +   +F  YL+W+C   VD S+PW A +S  +F V  + VP  SHF LACA+CD  HS
Sbjct: 33  QQDELHSFRKYLRWMC---VDHSSPWTAILSWTMFIVFTLVVPAISHFLLACADCDSYHS 92

Query: 90  RPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLDKLCEANRKVRDEYSKQLQRSME 149
           RP+  VVQLSLS+ AT+SF CL+ ++  YGLRRFLF DKL + +  VR  Y+ QL  S+ 
Sbjct: 93  RPYDSVVQLSLSSVATVSFLCLTRFVSKYGLRRFLFFDKLWDESETVRRNYTNQLNTSLH 152

Query: 150 LICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYISYITSCALELCSWLYRTSIFFFV 209
           ++ +F++PCF+A +AYK+WWY S  ++IP+ GN + +S   +C +ELCSWLYRT++ F V
Sbjct: 153 IVSYFVIPCFSAMSAYKIWWYASGGSRIPFLGNAV-LSDTVACIMELCSWLYRTTVIFLV 212

Query: 210 CILFRLVCRLQMVCLEDFGRAFHRETEVGAILRQHLGLRRTLRIVSHRFRVFMLLSLVLV 269
           C+LFRL+C LQ++ L+DF + F  +++VG+IL +HL +RR LRI+SHR+R F+L  L+LV
Sbjct: 213 CVLFRLICHLQILRLQDFAKLFQIDSDVGSILSEHLRIRRHLRIISHRYRSFILCLLILV 272

Query: 270 TASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGIFICLRSAAKITHKAQSITCLAAK 329
           T SQF SLL+TT++   VN+ +AG+LALCS++LVT + I LRSA+KITHKAQ++TCLAAK
Sbjct: 273 TGSQFSSLLITTKAYTEVNIYRAGELALCSMTLVTALLILLRSASKITHKAQAVTCLAAK 332

Query: 330 WHVAAIINTFD------DLDGETPT-AAR----------VMSTIVSNSDDEYEDDDDLDD 389
           WHV A + +FD      D   ETPT  AR          V++   S+SD+  +++DDLD+
Sbjct: 333 WHVCATLESFDQTVESFDQTVETPTLVARNNNDNNNVHDVVTLTESDSDEYGDEEDDLDN 392

Query: 390 AKLMPVFA-HTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKSIFAIELALVLWLLNK 437
             ++PV+A  T+SFQKRQALV Y  NN AGITVYGF +DR  L +IF +EL+LVLWLL K
Sbjct: 393 NDIIPVYAFSTMSFQKRQALVSYFENNSAGITVYGFTLDRGTLHTIFGLELSLVLWLLGK 452

BLAST of MC03g0525 vs. TAIR 10
Match: AT4G03820.1 (Protein of unknown function (DUF3537) )

HSP 1 Score: 392.1 bits (1006), Expect = 5.8e-109
Identity = 216/437 (49.43%), Postives = 302/437 (69.11%), Query Frame = 0

Query: 13  DSQQHQLLD----PEKSEAAAES-ESRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVL 72
           +S   Q+L+    P+  +   ES  ++ +F     W      DQSN  +  +S  +FF+L
Sbjct: 3   ESSNQQILNSETRPDPIQKPPESPATKLSFPRLFLWF-----DQSNRIKTLLSWSIFFLL 62

Query: 73  AIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLD 132
           A+ VP+ SHF L CA+CD  H RP+  +VQLSLS  A +SF  LS W + YG+RRFLF D
Sbjct: 63  AVIVPMISHFVLICADCDFKHRRPYDGLVQLSLSIFAGISFVSLSDWSKKYGIRRFLFFD 122

Query: 133 KLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYIS 192
           KL + + KVR  Y  ++QRSM+L+  F+LP  T +A Y++WWY S  NQIPY   N  +S
Sbjct: 123 KLKDVSDKVRIGYEAKIQRSMKLLAIFVLPSTTLQAIYRIWWYASGFNQIPYI-INPTLS 182

Query: 193 YITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRE-TEVGAILRQHLG 252
           ++ +C L+L SWLYRTS+F   CIL++ +C LQ++ L++F R F  E  +  +IL +HL 
Sbjct: 183 HVLACTLQLSSWLYRTSLFIIACILYQNICHLQVLRLDEFARCFASEIKDFSSILAEHLK 242

Query: 253 LRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGI 312
           +RR L+IVSHRFR F+LLSL  VTA+QF++LL T R++   N+ + G+LALCS SLV+G+
Sbjct: 243 IRRELKIVSHRFRRFILLSLFFVTATQFMALLTTIRASVPFNIYEVGELALCSTSLVSGL 302

Query: 313 FICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDL-DGETPTA------ARVMS---TI 372
           FICL+SA ++THKAQS+T +A KW+V A ++TFD L DGETP        ++++S    +
Sbjct: 303 FICLKSATQMTHKAQSVTSIATKWNVCASLDTFDVLYDGETPKCPTTTQHSQILSRRRNV 362

Query: 373 VSNSDDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKS 432
           V +SDD+ E + D +D ++ P+FA  IS QKRQALV YL NN+AGITVYGF+VD+TWL+ 
Sbjct: 363 VQSSDDDEEGEGDDNDLEIHPIFARAISSQKRQALVTYLENNRAGITVYGFLVDKTWLRM 422

Query: 433 IFAIELALVLWLLNKTV 434
           IF+IELAL+LWLL KT+
Sbjct: 423 IFSIELALLLWLLKKTI 433

BLAST of MC03g0525 vs. TAIR 10
Match: AT4G03820.2 (Protein of unknown function (DUF3537) )

HSP 1 Score: 392.1 bits (1006), Expect = 5.8e-109
Identity = 216/437 (49.43%), Postives = 302/437 (69.11%), Query Frame = 0

Query: 13  DSQQHQLLD----PEKSEAAAES-ESRSAFESYLKWICRIIVDQSNPWRASVSCFVFFVL 72
           +S   Q+L+    P+  +   ES  ++ +F     W      DQSN  +  +S  +FF+L
Sbjct: 3   ESSNQQILNSETRPDPIQKPPESPATKLSFPRLFLWF-----DQSNRIKTLLSWSIFFLL 62

Query: 73  AIAVPLASHFALACANCDEDHSRPFHVVVQLSLSAAATLSFACLSFWLRLYGLRRFLFLD 132
           A+ VP+ SHF L CA+CD  H RP+  +VQLSLS  A +SF  LS W + YG+RRFLF D
Sbjct: 63  AVIVPMISHFVLICADCDFKHRRPYDGLVQLSLSIFAGISFVSLSDWSKKYGIRRFLFFD 122

Query: 133 KLCEANRKVRDEYSKQLQRSMELICFFLLPCFTAEAAYKVWWYVSAANQIPYYGNNMYIS 192
           KL + + KVR  Y  ++QRSM+L+  F+LP  T +A Y++WWY S  NQIPY   N  +S
Sbjct: 123 KLKDVSDKVRIGYEAKIQRSMKLLAIFVLPSTTLQAIYRIWWYASGFNQIPYI-INPTLS 182

Query: 193 YITSCALELCSWLYRTSIFFFVCILFRLVCRLQMVCLEDFGRAFHRE-TEVGAILRQHLG 252
           ++ +C L+L SWLYRTS+F   CIL++ +C LQ++ L++F R F  E  +  +IL +HL 
Sbjct: 183 HVLACTLQLSSWLYRTSLFIIACILYQNICHLQVLRLDEFARCFASEIKDFSSILAEHLK 242

Query: 253 LRRTLRIVSHRFRVFMLLSLVLVTASQFISLLMTTRSNAHVNLSKAGQLALCSVSLVTGI 312
           +RR L+IVSHRFR F+LLSL  VTA+QF++LL T R++   N+ + G+LALCS SLV+G+
Sbjct: 243 IRRELKIVSHRFRRFILLSLFFVTATQFMALLTTIRASVPFNIYEVGELALCSTSLVSGL 302

Query: 313 FICLRSAAKITHKAQSITCLAAKWHVAAIINTFDDL-DGETPTA------ARVMS---TI 372
           FICL+SA ++THKAQS+T +A KW+V A ++TFD L DGETP        ++++S    +
Sbjct: 303 FICLKSATQMTHKAQSVTSIATKWNVCASLDTFDVLYDGETPKCPTTTQHSQILSRRRNV 362

Query: 373 VSNSDDEYEDDDDLDDAKLMPVFAHTISFQKRQALVVYLRNNKAGITVYGFMVDRTWLKS 432
           V +SDD+ E + D +D ++ P+FA  IS QKRQALV YL NN+AGITVYGF+VD+TWL+ 
Sbjct: 363 VQSSDDDEEGEGDDNDLEIHPIFARAISSQKRQALVTYLENNRAGITVYGFLVDKTWLRM 422

Query: 433 IFAIELALVLWLLNKTV 434
           IF+IELAL+LWLL KT+
Sbjct: 423 IFSIELALLLWLLKKTI 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_008458982.11.38e-23277.70PREDICTED: uncharacterized protein LOC103498231 [Cucumis melo][more]
XP_038893800.12.36e-23076.38uncharacterized protein LOC120082620 [Benincasa hispida][more]
XP_011660297.18.03e-22774.94uncharacterized protein LOC101203162 [Cucumis sativus][more]
XP_022954540.11.47e-22675.00uncharacterized protein LOC111456780 [Cucurbita moschata][more]
KAG6573486.12.97e-22678.66hypothetical protein SDJN03_27373, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A1S3C9496.66e-23377.70uncharacterized protein LOC103498231 OS=Cucumis melo OX=3656 GN=LOC103498231 PE=... [more]
A0A0A0M3M63.89e-22774.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G699550 PE=4 SV=1[more]
A0A6J1GSP87.13e-22775.00uncharacterized protein LOC111456780 OS=Cucurbita moschata OX=3662 GN=LOC1114567... [more]
A0A6J1K3141.66e-22677.03uncharacterized protein LOC111490209 OS=Cucurbita maxima OX=3661 GN=LOC111490209... [more]
A0A6J1EG161.69e-22176.19uncharacterized protein LOC111433990 OS=Cucurbita moschata OX=3662 GN=LOC1114339... [more]
Match NameE-valueIdentityDescription
AT4G22270.14.9e-12454.38Protein of unknown function (DUF3537) [more]
AT3G20300.15.4e-12353.54Protein of unknown function (DUF3537) [more]
AT1G50630.12.1e-11952.24Protein of unknown function (DUF3537) [more]
AT4G03820.15.8e-10949.43Protein of unknown function (DUF3537) [more]
AT4G03820.25.8e-10949.43Protein of unknown function (DUF3537) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021924Protein of unknown function DUF3537PFAMPF12056DUF3537coord: 35..420
e-value: 1.3E-153
score: 511.5
IPR021924Protein of unknown function DUF3537PANTHERPTHR31963RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR Kcoord: 23..436
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR31963:SF17PROTEIN, PUTATIVE (DUF3537)-RELATEDcoord: 23..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0525.1MC03g0525.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane