MC03g0512 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0512
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Oxoglutarate/iron-dependent dioxygenase
Location	MC03: 11948048 .. 11950931 (+)
RNA-Seq Expression	MC03g0512
Synteny	MC03g0512

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTATTAGATTAGACTTTAGAGAACCGAGTACCCTCTACTCTGAGTACCCATTGTTATTGGTTCCGCTCTCTTCTGATTACCAGATTCCAGTTCCAAGACCATTGAACTTCCTTCCGCTTCAAGTTCCTCCGAAATTCTGAATTTGATCGACCTCCACCGCCTTACAAGTTACTACCAATTCAGCCCGGTTCCTCTCAAGATCATGAATCGCGGTCGCCCTGGATATGGAAGACCCCATCCCAATAGCAGAGGACGATCTTTTCGCTCCGCCGATAATTATTCCTATCGTCCCCGCCATGTAACTTTCCTTAATCTCCTCAATGTTTACTCACTTTCGCGTATTCCCTGTTCTCTGATCTTTCTTTTGTTTATTTATTCCTGCCCATCAATTTGACTGTTCGGATTCTATTTCAGTAGAGGAGGGATTTGAATGGATTACCCATTTGTGTTTTTGTGTTGCCGTGTGCCACTGGTGGATTACAATAATGGCACTAGGAAGATTAATGGGTTACTTGTTTATCTCTGGAATTTTGAAATTTGAAGTTTGTTTGTAAATAAATGAAAATGTAGTGAATGACGAAGGTGCTGGAGTTTAGATTTTTACTCTCACGCTCACTTCTATTCTGTTCTTTTCACATTCTAACACAAAATTCAGGCTGGAGGTGAGGGGCCTTCTCTTGATGGTTCTTCTAACCCTAGCAGTGGTGATTTCCGAGGCAGAAGTTCTCAACAGATGCCGTCAAGGAATTTTAGGAAGAATTATGGGCAGAATCAGGCCTTGAGTTCTGAACAGTGGCAATGGCGGCCTTTAAATAGTGGGAAAGATGCTTCTCCTTCTCCTGGCGCTGTGGATCTTCAGCAGGAACATAGTTCAGCTGATGGTGCAGGCAATGCCTCTGAACAATTGTTGGCATCTACGGAATCAAATTCTAATAGCAACGAACTCTCACCGTCATCTGCTCTAAATGTCTCTAAAGATTTGCATTCTGCTGTAGAAAGAATTCAGATTCGAGAACCTACAACAGAAGGGGAAAGTTGCAGTAGTTTATTGCCTTATGATAATTGTAACAGATTAGCTGCTGTTGACCAGGAACTCAAGGTTCAAGTTCCCTTGGAATCCTGTGCTAAAGAAGAGAGTTCCTCCATAAAATCAAAGGAAAATAATGTTTCTAACTGTAAGGGTTCTGAGGACAACAATAAGCCTTCAGTGAACCTTGATCCTTTTGATATTTGCCCCCCAAAGTCTGGACGTATTACGCTGAATCCTTCTTTATTAGCTACGAACAAAGAAAAGAGGAACGAGACGAAGCGCACAACGGAGGGAAACTGTGGAGTTGTGTTGAGACCAGGCATGGTTCTTCTGAAGAGTGGAATTTCCCTCAGGGATCAGGTATACATTAATTTTCACTCTCTTAGACTTACATACATAAATGAATAATGTATATCAAAATCTTGTTATTGTAGAATAGCTCGAACATAACTGAACCAGTTCTTGAGTTAAATGTGTTTCCATATATACATAATGAGGGAACGCTCACCTTCCCAGGACCGGGAAGTGCTTTATCATCAAGCAGTAAATGGATTTTATTAAGTTGGGTTGTAGTTTTCTTTCACTATGGTCTGTGATTTGCGTTGAGTCTAGGATCATCATGCAGATGTTGGATGGTTTTGTTTCTGTTGTTTGATTGGCATCACACTATTTTTTCTGGCTCTGTTGGTAGTATTTTCAAACTCTCTAAGACCTGCTTTATTATTTTCTATCTTTATTATGCTCGCAGGTGCAGATAGTAAAAAGATGTCGGAACCTTGGTCTAGGCGCTGGAGGTTTTTACCAACCCGGTTACCGCGAAGGAGGAAAATTACACTTGAAGATGATGTGCCTTGGTAAAAATTGGGATCCTGACACTAGTACTTATGGGGATGTCCGTCCATTTGATGATACAAAACCACCACGCATTCCAGTTGAATTTCACCAATTGGTTGAGAAGGCAATCAAAGATTCTTATGCCATTATGGGAAAAGATTCAACAGCCAAAACTCCTGAACGTGTACTTCCATGGATGGAACCGAACATCTGTATCGTAAACTTTTACTCGCAAAATGGGCGATTGGGTCTTCATCAGGTCTGCATATAACTCCTCTGCTCTTCATTGCTCTTCATTTGCATACTTTTTCCATGACAATCTCTTTACATCCCATACAATGACCCTTTGACATTGAGTTATTTTAATATACTCCCATTACATTAGGATCGAGATGAAAGTCGAGAAAGTCTCGAAAAGGGGTTGCCTGTTGTTTCCTTCTCCATTGGCGACTCTGCAGAATTCCTATATGGTGATCGAAGTGACATCGATCAGGCGGAGAAAGTCACTCTGAAATCGGGAGATATTTTGATATTTGGTGGAAAATCAAGACATGTTTTTCATGGGGTGACTGAAATTCATCCAAACACTGCTCCGAAAGCACTTTTAGAAGAAACAAATCTTCGTTCGGGTCGCCTGAATCTTACTTTCCGTCAATACTGAAAAACAGTCTTCTGCTTAGCAAAGGAGATATCCATGAACCGACGCTGTTAAGGTGGGGATTTTAACCCTCGAACTTTTATATATATAGACGTTGGGGATTTGAACCTCCGAACTTTTATATATATAGGCTTTGGACCAATACAAATGTTTTAGTTGTGGTATGATCATCTAATCTTGAGACATGCTAGAGTATGTTTAAATGCTGAGTTTTGTAATTACTTGTAAATACCCTGAACAGCCTGGGTAAATACTACTCTCTGTGTGGAGAGCCCAATATTTTTGAATTGTAATCTCTGATATCCTAAACTAGTTAAATAATATAAAAGTAGCCTGAATTATAGCTTAGCTTCAATTATG

mRNA sequence

CTTTATTAGATTAGACTTTAGAGAACCGAGTACCCTCTACTCTGAGTACCCATTGTTATTGGTTCCGCTCTCTTCTGATTACCAGATTCCAGTTCCAAGACCATTGAACTTCCTTCCGCTTCAAGTTCCTCCGAAATTCTGAATTTGATCGACCTCCACCGCCTTACAAGTTACTACCAATTCAGCCCGGTTCCTCTCAAGATCATGAATCGCGGTCGCCCTGGATATGGAAGACCCCATCCCAATAGCAGAGGACGATCTTTTCGCTCCGCCGATAATTATTCCTATCGTCCCCGCCATGCTGGAGGTGAGGGGCCTTCTCTTGATGGTTCTTCTAACCCTAGCAGTGGTGATTTCCGAGGCAGAAGTTCTCAACAGATGCCGTCAAGGAATTTTAGGAAGAATTATGGGCAGAATCAGGCCTTGAGTTCTGAACAGTGGCAATGGCGGCCTTTAAATAGTGGGAAAGATGCTTCTCCTTCTCCTGGCGCTGTGGATCTTCAGCAGGAACATAGTTCAGCTGATGGTGCAGGCAATGCCTCTGAACAATTGTTGGCATCTACGGAATCAAATTCTAATAGCAACGAACTCTCACCGTCATCTGCTCTAAATGTCTCTAAAGATTTGCATTCTGCTGTAGAAAGAATTCAGATTCGAGAACCTACAACAGAAGGGGAAAGTTGCAGTAGTTTATTGCCTTATGATAATTGTAACAGATTAGCTGCTGTTGACCAGGAACTCAAGGTTCAAGTTCCCTTGGAATCCTGTGCTAAAGAAGAGAGTTCCTCCATAAAATCAAAGGAAAATAATGTTTCTAACTGTAAGGGTTCTGAGGACAACAATAAGCCTTCAGTGAACCTTGATCCTTTTGATATTTGCCCCCCAAAGTCTGGACGTATTACGCTGAATCCTTCTTTATTAGCTACGAACAAAGAAAAGAGGAACGAGACGAAGCGCACAACGGAGGGAAACTGTGGAGTTGTGTTGAGACCAGGCATGGTTCTTCTGAAGAGTGGAATTTCCCTCAGGGATCAGGTGCAGATAGTAAAAAGATGTCGGAACCTTGGTCTAGGCGCTGGAGGTTTTTACCAACCCGGTTACCGCGAAGGAGGAAAATTACACTTGAAGATGATGTGCCTTGGTAAAAATTGGGATCCTGACACTAGTACTTATGGGGATGTCCGTCCATTTGATGATACAAAACCACCACGCATTCCAGTTGAATTTCACCAATTGGTTGAGAAGGCAATCAAAGATTCTTATGCCATTATGGGAAAAGATTCAACAGCCAAAACTCCTGAACGTGTACTTCCATGGATGGAACCGAACATCTGTATCGTAAACTTTTACTCGCAAAATGGGCGATTGGGTCTTCATCAGGATCGAGATGAAAGTCGAGAAAGTCTCGAAAAGGGGTTGCCTGTTGTTTCCTTCTCCATTGGCGACTCTGCAGAATTCCTATATGGTGATCGAAGTGACATCGATCAGGCGGAGAAAGTCACTCTGAAATCGGGAGATATTTTGATATTTGGTGGAAAATCAAGACATGTTTTTCATGGGGTGACTGAAATTCATCCAAACACTGCTCCGAAAGCACTTTTAGAAGAAACAAATCTTCGTTCGGGTCGCCTGAATCTTACTTTCCGTCAATACTGAAAAACAGTCTTCTGCTTAGCAAAGGAGATATCCATGAACCGACGCTGTTAAGGTGGGGATTTTAACCCTCGAACTTTTATATATATAGACGTTGGGGATTTGAACCTCCGAACTTTTATATATATAGGCTTTGGACCAATACAAATGTTTTAGTTGTGGTATGATCATCTAATCTTGAGACATGCTAGAGTATGTTTAAATGCTGAGTTTTGTAATTACTTGTAAATACCCTGAACAGCCTGGGTAAATACTACTCTCTGTGTGGAGAGCCCAATATTTTTGAATTGTAATCTCTGATATCCTAAACTAGTTAAATAATATAAAAGTAGCCTGAATTATAGCTTAGCTTCAATTATG

Coding sequence (CDS)

ATGAATCGCGGTCGCCCTGGATATGGAAGACCCCATCCCAATAGCAGAGGACGATCTTTTCGCTCCGCCGATAATTATTCCTATCGTCCCCGCCATGCTGGAGGTGAGGGGCCTTCTCTTGATGGTTCTTCTAACCCTAGCAGTGGTGATTTCCGAGGCAGAAGTTCTCAACAGATGCCGTCAAGGAATTTTAGGAAGAATTATGGGCAGAATCAGGCCTTGAGTTCTGAACAGTGGCAATGGCGGCCTTTAAATAGTGGGAAAGATGCTTCTCCTTCTCCTGGCGCTGTGGATCTTCAGCAGGAACATAGTTCAGCTGATGGTGCAGGCAATGCCTCTGAACAATTGTTGGCATCTACGGAATCAAATTCTAATAGCAACGAACTCTCACCGTCATCTGCTCTAAATGTCTCTAAAGATTTGCATTCTGCTGTAGAAAGAATTCAGATTCGAGAACCTACAACAGAAGGGGAAAGTTGCAGTAGTTTATTGCCTTATGATAATTGTAACAGATTAGCTGCTGTTGACCAGGAACTCAAGGTTCAAGTTCCCTTGGAATCCTGTGCTAAAGAAGAGAGTTCCTCCATAAAATCAAAGGAAAATAATGTTTCTAACTGTAAGGGTTCTGAGGACAACAATAAGCCTTCAGTGAACCTTGATCCTTTTGATATTTGCCCCCCAAAGTCTGGACGTATTACGCTGAATCCTTCTTTATTAGCTACGAACAAAGAAAAGAGGAACGAGACGAAGCGCACAACGGAGGGAAACTGTGGAGTTGTGTTGAGACCAGGCATGGTTCTTCTGAAGAGTGGAATTTCCCTCAGGGATCAGGTGCAGATAGTAAAAAGATGTCGGAACCTTGGTCTAGGCGCTGGAGGTTTTTACCAACCCGGTTACCGCGAAGGAGGAAAATTACACTTGAAGATGATGTGCCTTGGTAAAAATTGGGATCCTGACACTAGTACTTATGGGGATGTCCGTCCATTTGATGATACAAAACCACCACGCATTCCAGTTGAATTTCACCAATTGGTTGAGAAGGCAATCAAAGATTCTTATGCCATTATGGGAAAAGATTCAACAGCCAAAACTCCTGAACGTGTACTTCCATGGATGGAACCGAACATCTGTATCGTAAACTTTTACTCGCAAAATGGGCGATTGGGTCTTCATCAGGATCGAGATGAAAGTCGAGAAAGTCTCGAAAAGGGGTTGCCTGTTGTTTCCTTCTCCATTGGCGACTCTGCAGAATTCCTATATGGTGATCGAAGTGACATCGATCAGGCGGAGAAAGTCACTCTGAAATCGGGAGATATTTTGATATTTGGTGGAAAATCAAGACATGTTTTTCATGGGGTGACTGAAATTCATCCAAACACTGCTCCGAAAGCACTTTTAGAAGAAACAAATCTTCGTTCGGGTCGCCTGAATCTTACTTTCCGTCAATACTGA

Protein sequence

MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMPSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLASTESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTFRQY

Homology

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BLAST of MC03g0512 vs. ExPASy Swiss-Prot
Match: P0CAT7 (Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=alkB PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 2.0e-14
Identity = 60/196 (30.61%), Postives = 84/196 (42.86%), Query Frame = 0


Query: 295 YQPGYREGGKLHLKMMCLGK-NWDPDTSTYGDV-------RPFDDTKPPRIPVEFHQLVE 354
           Y+  Y  G  + + M  LG   W  D   Y  V       RP+ D  P            
Sbjct: 57  YRTAY--GKPMSVAMTALGSLGWTSDARGYRYVDRHPETGRPWPDMPP------------ 116

Query: 355 KAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLP 414
            A+ D + ++G   T            P+ C+VN Y    R+GLHQDRDE+    +   P
Sbjct: 117 -ALLDLWTVLGDPET-----------PPDSCLVNLYRDGARMGLHQDRDEA----DPRFP 176

Query: 415 VVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALL 474
           V+S S+GD+A F  G  +  D    + L SGD+    G +R  FHGV  I P ++     
Sbjct: 177 VLSISLGDTAVFRIGGVNRKDPTRSLRLASGDVCRLLGPARLAFHGVDRILPGSSSLV-- 216

Query: 475 EETNLRSGRLNLTFRQ 483
                  GR+NLT R+
Sbjct: 237 ----PGGGRINLTLRR 216

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BLAST of MC03g0512 vs. ExPASy Swiss-Prot
Match: B8GWW6 (Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=alkB PE=3 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 2.0e-14
Identity = 60/196 (30.61%), Postives = 84/196 (42.86%), Query Frame = 0


Query: 295 YQPGYREGGKLHLKMMCLGK-NWDPDTSTYGDV-------RPFDDTKPPRIPVEFHQLVE 354
           Y+  Y  G  + + M  LG   W  D   Y  V       RP+ D  P            
Sbjct: 57  YRTAY--GKPMSVAMTALGSLGWTSDARGYRYVDRHPETGRPWPDMPP------------ 116

Query: 355 KAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLP 414
            A+ D + ++G   T            P+ C+VN Y    R+GLHQDRDE+    +   P
Sbjct: 117 -ALLDLWTVLGDPET-----------PPDSCLVNLYRDGARMGLHQDRDEA----DPRFP 176

Query: 415 VVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALL 474
           V+S S+GD+A F  G  +  D    + L SGD+    G +R  FHGV  I P ++     
Sbjct: 177 VLSISLGDTAVFRIGGVNRKDPTRSLRLASGDVCRLLGPARLAFHGVDRILPGSSSLV-- 216

Query: 475 EETNLRSGRLNLTFRQ 483
                  GR+NLT R+
Sbjct: 237 ----PGGGRINLTLRR 216

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BLAST of MC03g0512 vs. ExPASy Swiss-Prot
Match: P05050 (Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Escherichia coli (strain K12) OX=83333 GN=alkB PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 5.8e-14
Identity = 48/160 (30.00%), Postives = 78/160 (48.75%), Query Frame = 0


Query: 323 YGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFY 382
           Y  + P  +   P +P  FH L ++A           +TA       P  +P+ C++N Y
Sbjct: 78  YSPIDPQTNKPWPAMPQSFHNLCQRA-----------ATAAG----YPDFQPDACLINRY 137

Query: 383 SQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIF 442
           +   +L LHQD+DE     +   P+VS S+G  A F +G     D  +++ L+ GD++++
Sbjct: 138 APGAKLSLHQDKDEP----DLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVW 197

Query: 443 GGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTFRQ 483
           GG+SR  +HG+  +     P  +         R NLTFRQ
Sbjct: 198 GGESRLFYHGIQPLKAGFHPLTI-------DCRYNLTFRQ 211

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BLAST of MC03g0512 vs. ExPASy Swiss-Prot
Match: P37462 (Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=alkB PE=3 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 2.9e-13
Identity = 53/176 (30.11%), Postives = 79/176 (44.89%), Query Frame = 0


Query: 312 LGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLV-EKAIKDSYAIMGKDSTAKTPERVLP 371
           LG   D     Y    P  D   P +P+ F  +  + AI   YA                
Sbjct: 67  LGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYAS--------------- 126

Query: 372 WMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAE 431
             +P+ C++N Y+   +L LHQD+DE     +   P+VS S+G  A F +G     D  +
Sbjct: 127 -FQPDACLINRYAPGAKLSLHQDKDEP----DLRAPIVSVSLGVPAVFQFGGLRRSDPIQ 186

Query: 432 KVTLKSGDILIFGGKSRHVFHGVTEI----HPNTAPKALLEETNLRSGRLNLTFRQ 483
           ++ L+ GDI+++GG+SR  +HG+  +    HP T              R NLTFRQ
Sbjct: 187 RILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMTG-----------EFRYNLTFRQ 211

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BLAST of MC03g0512 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.0e-10
Identity = 34/92 (36.96%), Postives = 57/92 (61.96%), Query Frame = 0


Query: 379 VNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGD 438
           VNFYS++  +G H   D++ + +EK  P++S S G +A FL G  +       + ++SGD
Sbjct: 266 VNFYSEDSIMGGH--LDDAEQEMEK--PIISISFGSTAVFLMGAETRDIAPVPLFIRSGD 325

Query: 439 ILIFGGKSRHVFHGVTEIHPNTAPKALLEETN 471
           I+I GG+SR+ +HGV +I  N+    L++E +
Sbjct: 326 IVIMGGRSRYCYHGVAKIVENSFDLGLIDEND 353

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BLAST of MC03g0512 vs. NCBI nr
Match: XP_022142665.1 (uncharacterized protein LOC111012720 [Momordica charantia])

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0


Query: 1   MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMP 60
           MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMP
Sbjct: 1   MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMP 60

Query: 61  SRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAST 120
           SRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAST
Sbjct: 61  SRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAST 120

Query: 121 ESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELK 180
           ESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELK
Sbjct: 121 ESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELK 180

Query: 181 VQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLA 240
           VQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLA
Sbjct: 181 VQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLA 240

Query: 241 TNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYR 300
           TNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYR
Sbjct: 241 TNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYR 300

Query: 301 EGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDS 360
           EGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDS
Sbjct: 301 EGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDS 360

Query: 361 TAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLY 420
           TAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLY
Sbjct: 361 TAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLY 420

Query: 421 GDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTF 480
           GDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTF
Sbjct: 421 GDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTF 480

Query: 481 RQY 483
           RQY
Sbjct: 481 RQY 483

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BLAST of MC03g0512 vs. NCBI nr
Match: XP_038875730.1 (uncharacterized protein LOC120068103 isoform X1 [Benincasa hispida])

HSP 1 Score: 715 bits (1846), Expect = 1.58e-255
Identity = 379/501 (75.65%), Postives = 411/501 (82.04%), Query Frame = 0


Query: 4   GRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMPSRN 63
           GR   GR HPN+RGRS RSADN++YRPRHAGGEGPS+ GSSNP +G FR RSSQ M SRN
Sbjct: 5   GRRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGPSVAGSSNPGNGTFRDRSSQHMSSRN 64

Query: 64  FRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLASTESN 123
             K +GQ QA SSEQWQWRPLNSGKDAS +  A+DLQ EH+SAD   N S+QLL S  SN
Sbjct: 65  VGKPFGQKQASSSEQWQWRPLNSGKDASSA--AIDLQPEHNSADDLSNTSKQLLGSIASN 124

Query: 124 SNSN--------------------ELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSL 183
           ++SN                    E SPSSA NVSK LHSAVERIQIRE T  GESCS  
Sbjct: 125 TDSNHMSKSSKQLLGSIASNSNCKEFSPSSAQNVSKSLHSAVERIQIRESTAVGESCSDS 184

Query: 184 LPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVNLDPF 243
            P+DNCNR  AV Q+LKVQV LESC K+ESS+ K +E NNVS    S+D  KPSVNLDPF
Sbjct: 185 FPHDNCNRSDAVGQDLKVQVLLESCVKDESSTKKLRESNNVSGSTDSKDK-KPSVNLDPF 244

Query: 244 DICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVK 303
           DIC PK+G +TLNPSL A N+EKRNE KR  EGN G+VLRPGMV LKSGISLRDQV IVK
Sbjct: 245 DICLPKTGVVTLNPSLFAKNREKRNEMKRAMEGNSGIVLRPGMVHLKSGISLRDQVMIVK 304

Query: 304 RCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFH 363
           RCR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+STYGDVRPFDDTKPP +P EF+
Sbjct: 305 RCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPSLPDEFY 364

Query: 364 QLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLE 423
           QLVEKAIK SYAIMGKDST K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDESRESL+
Sbjct: 365 QLVEKAIKVSYAIMGKDSTMKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESRESLD 424

Query: 424 KGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAP 483
           KGLPVVSFSIGDSAEFL+GD+SD DQAEKVTL+SGDILIFGGKSRHVFHGVT IHPNTAP
Sbjct: 425 KGLPVVSFSIGDSAEFLFGDQSDADQAEKVTLESGDILIFGGKSRHVFHGVTVIHPNTAP 484

[Close]

BLAST of MC03g0512 vs. NCBI nr
Match: KAA0058530.1 (Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa] >TYK07216.1 Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 692 bits (1785), Expect = 3.01e-246
Identity = 369/505 (73.07%), Postives = 407/505 (80.59%), Query Frame = 0


Query: 1   MNRGRPGY-GRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G P Y GR HPN+RGRS RSADN++YRPRHAGGEG S+ GSSNP SG FRGRSS QM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAS 120
            SRNFRK  GQ QA SSEQWQWRPLNS KDASP  GAVDL  +H+S D   N ++QLL S
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASP--GAVDLHLQHNSTDDMSNTNKQLLGS 120

Query: 121 TESNSNSNELS--------------------PSSALNVSKDLHSAVERIQIREPTTEGES 180
             SNS+SN++S                     SSA NVSK LHSAVERIQI+ PT    +
Sbjct: 121 IASNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGN 180

Query: 181 CSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVN 240
           CS   PYDN N    V QELKVQ  LESCAK++SS+IK  E NNV N   S+D  KPSV+
Sbjct: 181 CSDSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDK-KPSVD 240

Query: 241 LDPFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQV 300
           LD FDICPPK+G +TLNPSLLA N+EKRNE KR  +GN G+VLRPGMV LK  ISLRDQ 
Sbjct: 241 LDTFDICPPKTGGVTLNPSLLAMNREKRNEMKRAMDGNNGIVLRPGMVHLKGSISLRDQA 300

Query: 301 QIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIP 360
           +IVK+CR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+STYGDVRPFDDTKPP +P
Sbjct: 301 KIVKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLP 360

Query: 361 VEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESR 420
            EF+QLVEKAIKDSYAI+ KDST K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDES+
Sbjct: 361 DEFYQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQ 420

Query: 421 ESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHP 480
           ESL+KGLPV+SFSIGDSAEFL+GD SD+DQAEKVTL+SGDILIFGGKSRHVFHGVT IH 
Sbjct: 421 ESLDKGLPVISFSIGDSAEFLFGDWSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHS 480

Query: 481 NTAPKALLEETNLRSGRLNLTFRQY 483
           NTAPKALLE TNLR GRLNLTFRQY
Sbjct: 481 NTAPKALLEATNLRPGRLNLTFRQY 502

[Close]

BLAST of MC03g0512 vs. NCBI nr
Match: XP_016903133.1 (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 [Cucumis melo])

HSP 1 Score: 686 bits (1770), Expect = 5.54e-244
Identity = 368/505 (72.87%), Postives = 406/505 (80.40%), Query Frame = 0


Query: 1   MNRGRPGY-GRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G P Y GR HPN+RGRS RSADN++YRPRHAGGEG S+ GSSNP SG FRGRSS QM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAS 120
            SRNFRK  GQ QA SSEQWQWRPLNS KDASP  GAVDL  +H+S D   N ++QLL S
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASP--GAVDLHLQHNSTDDMSNTNKQLLGS 120

Query: 121 TESNSNSNELS--------------------PSSALNVSKDLHSAVERIQIREPTTEGES 180
             SNS+SN++S                     SSA NVSK LHSAVERIQI+ PT    +
Sbjct: 121 IASNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGN 180

Query: 181 CSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVN 240
           CS   PYDN N    V QELKVQ  LESCAK++SS+IK  E NNV N   S+D  KPSV+
Sbjct: 181 CSDSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDK-KPSVD 240

Query: 241 LDPFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQV 300
           LD FDICPPK+G +TLNPSLLA N+EK NE KR  +GN G+VLRPGMV LK  ISLRDQ 
Sbjct: 241 LDTFDICPPKTGGVTLNPSLLAMNREK-NEMKRAMDGNNGIVLRPGMVHLKGSISLRDQA 300

Query: 301 QIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIP 360
           +IVK+CR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+STYGDVRPFDDTKPP +P
Sbjct: 301 KIVKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLP 360

Query: 361 VEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESR 420
            EF+QLVEKAIKDSYAI+ KDST K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDES+
Sbjct: 361 DEFYQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQ 420

Query: 421 ESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHP 480
           ESL+KGLPV+SFSIGDSAEFL+GDRSD+DQAEKVTL+SGDILIFGGKSRHVFHGVT IH 
Sbjct: 421 ESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHS 480

Query: 481 NTAPKALLEETNLRSGRLNLTFRQY 483
            TAPKALLE TNLR GRLNLTFRQY
Sbjct: 481 KTAPKALLEATNLRPGRLNLTFRQY 501

[Close]

BLAST of MC03g0512 vs. NCBI nr
Match: KAG6581809.1 (hypothetical protein SDJN03_21811, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 679 bits (1753), Expect = 1.43e-241
Identity = 365/503 (72.56%), Postives = 403/503 (80.12%), Query Frame = 0


Query: 1   MNRGRPGYG-RPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G   YG R HPN+RGR  RSADN++YRPRHAGGEGPS  GSSNP +G F+ RSS   
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSH-- 60

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAG--------- 120
           PSRN R  +GQ QAL SEQWQWRPLNSGKDAS   GAVDLQQ+H++AD            
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDASA--GAVDLQQDHNTADDIAPKQLIGSIS 120

Query: 121 ---------NASEQLLASTESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCS 180
                    N SEQLL S  S S+ NE SPSSA NVSK LHSAVERIQIREPT EGE C+
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCN 180

Query: 181 SLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVNLD 240
              PYD+C R  A  QEL VQ        +++++IK +E NNVS+CK S+D  KP  NL+
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDK-KPLGNLE 240

Query: 241 PFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQI 300
            FDICPPKSG +TLNPSLL+ N+EKRNE KR  EGN G VLRPGMV LKSGISL DQV+I
Sbjct: 241 IFDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKI 300

Query: 301 VKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVE 360
           VK+CR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+S YGDVRPFD+T PP +PVE
Sbjct: 301 VKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVE 360

Query: 361 FHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRES 420
           F++LVEKAIKDSYA+MGKDS  K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDES+ES
Sbjct: 361 FYKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQES 420

Query: 421 LEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNT 480
           LEKGLPVVSFSIGDSAEFL+GDRSDIDQAEKVTL+SGDILIFGGKSRHVFHGVT IH NT
Sbjct: 421 LEKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNT 480

Query: 481 APKALLEETNLRSGRLNLTFRQY 483
           APKALLE TNLR GRLNLTFRQY
Sbjct: 481 APKALLEATNLRPGRLNLTFRQY 490

[Close]

BLAST of MC03g0512 vs. ExPASy TrEMBL
Match: A0A6J1CLK3 (uncharacterized protein LOC111012720 OS=Momordica charantia OX=3673 GN=LOC111012720 PE=4 SV=1)

HSP 1 Score: 960 bits (2481), Expect = 0.0
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0


Query: 1   MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMP 60
           MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMP
Sbjct: 1   MNRGRPGYGRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQMP 60

Query: 61  SRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAST 120
           SRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAST
Sbjct: 61  SRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAST 120

Query: 121 ESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELK 180
           ESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELK
Sbjct: 121 ESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELK 180

Query: 181 VQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLA 240
           VQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLA
Sbjct: 181 VQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLA 240

Query: 241 TNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYR 300
           TNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYR
Sbjct: 241 TNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYR 300

Query: 301 EGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDS 360
           EGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDS
Sbjct: 301 EGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDS 360

Query: 361 TAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLY 420
           TAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLY
Sbjct: 361 TAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLY 420

Query: 421 GDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTF 480
           GDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTF
Sbjct: 421 GDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNLTF 480

Query: 481 RQY 483
           RQY
Sbjct: 481 RQY 483

[Close]

BLAST of MC03g0512 vs. ExPASy TrEMBL
Match: A0A0A0LC72 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G651830 PE=4 SV=1)

HSP 1 Score: 698 bits (1801), Expect = 1.72e-248
Identity = 364/485 (75.05%), Postives = 406/485 (83.71%), Query Frame = 0


Query: 1   MNRGRPGY-GRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G P Y GR HPN+RGRS RSAD+++YRPRHAGGEG S+ GSSNP SG FRGRSS QM
Sbjct: 53  MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 112

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAS 120
            SRNFRK  GQ QA SSEQWQWRPLNSGKDASP  GAVDLQ +H+S D   N ++QLL S
Sbjct: 113 SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASP--GAVDLQLQHNSTDDMSNNNKQLLES 172

Query: 121 TESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQEL 180
             SNS+  ELS SSA NVSK LHSAVERI ++ PT    S     PYDNCNR   V QEL
Sbjct: 173 IASNSDCIELSSSSAQNVSKSLHSAVERIHVQGPTAVCGSYGDSFPYDNCNRSDVVGQEL 232

Query: 181 KVQVPLESCAKEESSSIK-SKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSL 240
           KVQ  L+SCAK+ES +I+  K N+V N   S+D  KPSV+LD FDICPPK+G + LNPSL
Sbjct: 233 KVQPSLKSCAKDESFTIQLGKSNDVFNSTDSKDK-KPSVDLDSFDICPPKTGGVMLNPSL 292

Query: 241 LATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPG 300
           LA N+EKRNE +R  EGN G+VLRPGMV LK GIS+RDQ +IVK+CR+LG+GAGGFYQPG
Sbjct: 293 LAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISVRDQAKIVKKCRDLGIGAGGFYQPG 352

Query: 301 YREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGK 360
           YREGGKLHLKMMCLGKNWDPD+STYGD+RPFDDTKPP +P EF+QLVEKAIKDSYAIM +
Sbjct: 353 YREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEFYQLVEKAIKDSYAIMAE 412

Query: 361 DSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEF 420
           DST K PERVLPWM+P+ICIVNFYSQNGRLGLHQDRDES+ESL+KGLPV+SFSIGDSAEF
Sbjct: 413 DSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEF 472

Query: 421 LYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALLEETNLRSGRLNL 480
           L+GDRSD+DQAEKVTL+SGDILIFGGKSRHVFHGVT IH NTAPKALLE TNLR GRLNL
Sbjct: 473 LFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLNL 532

Query: 481 TFRQY 483
           TFRQY
Sbjct: 533 TFRQY 534

[Close]

BLAST of MC03g0512 vs. ExPASy TrEMBL
Match: A0A5D3CA69 (Oxoglutarate/iron-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G001030 PE=4 SV=1)

HSP 1 Score: 692 bits (1785), Expect = 1.46e-246
Identity = 369/505 (73.07%), Postives = 407/505 (80.59%), Query Frame = 0


Query: 1   MNRGRPGY-GRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G P Y GR HPN+RGRS RSADN++YRPRHAGGEG S+ GSSNP SG FRGRSS QM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAS 120
            SRNFRK  GQ QA SSEQWQWRPLNS KDASP  GAVDL  +H+S D   N ++QLL S
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASP--GAVDLHLQHNSTDDMSNTNKQLLGS 120

Query: 121 TESNSNSNELS--------------------PSSALNVSKDLHSAVERIQIREPTTEGES 180
             SNS+SN++S                     SSA NVSK LHSAVERIQI+ PT    +
Sbjct: 121 IASNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGN 180

Query: 181 CSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVN 240
           CS   PYDN N    V QELKVQ  LESCAK++SS+IK  E NNV N   S+D  KPSV+
Sbjct: 181 CSDSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDK-KPSVD 240

Query: 241 LDPFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQV 300
           LD FDICPPK+G +TLNPSLLA N+EKRNE KR  +GN G+VLRPGMV LK  ISLRDQ 
Sbjct: 241 LDTFDICPPKTGGVTLNPSLLAMNREKRNEMKRAMDGNNGIVLRPGMVHLKGSISLRDQA 300

Query: 301 QIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIP 360
           +IVK+CR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+STYGDVRPFDDTKPP +P
Sbjct: 301 KIVKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLP 360

Query: 361 VEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESR 420
            EF+QLVEKAIKDSYAI+ KDST K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDES+
Sbjct: 361 DEFYQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQ 420

Query: 421 ESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHP 480
           ESL+KGLPV+SFSIGDSAEFL+GD SD+DQAEKVTL+SGDILIFGGKSRHVFHGVT IH 
Sbjct: 421 ESLDKGLPVISFSIGDSAEFLFGDWSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHS 480

Query: 481 NTAPKALLEETNLRSGRLNLTFRQY 483
           NTAPKALLE TNLR GRLNLTFRQY
Sbjct: 481 NTAPKALLEATNLRPGRLNLTFRQY 502

[Close]

BLAST of MC03g0512 vs. ExPASy TrEMBL
Match: A0A1S4E4H2 (LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 OS=Cucumis melo OX=3656 GN=LOC103501985 PE=4 SV=1)

HSP 1 Score: 686 bits (1770), Expect = 2.68e-244
Identity = 368/505 (72.87%), Postives = 406/505 (80.40%), Query Frame = 0


Query: 1   MNRGRPGY-GRPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G P Y GR HPN+RGRS RSADN++YRPRHAGGEG S+ GSSNP SG FRGRSS QM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAGNASEQLLAS 120
            SRNFRK  GQ QA SSEQWQWRPLNS KDASP  GAVDL  +H+S D   N ++QLL S
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASP--GAVDLHLQHNSTDDMSNTNKQLLGS 120

Query: 121 TESNSNSNELS--------------------PSSALNVSKDLHSAVERIQIREPTTEGES 180
             SNS+SN++S                     SSA NVSK LHSAVERIQI+ PT    +
Sbjct: 121 IASNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGN 180

Query: 181 CSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVN 240
           CS   PYDN N    V QELKVQ  LESCAK++SS+IK  E NNV N   S+D  KPSV+
Sbjct: 181 CSDSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDK-KPSVD 240

Query: 241 LDPFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQV 300
           LD FDICPPK+G +TLNPSLLA N+EK NE KR  +GN G+VLRPGMV LK  ISLRDQ 
Sbjct: 241 LDTFDICPPKTGGVTLNPSLLAMNREK-NEMKRAMDGNNGIVLRPGMVHLKGSISLRDQA 300

Query: 301 QIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIP 360
           +IVK+CR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+STYGDVRPFDDTKPP +P
Sbjct: 301 KIVKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLP 360

Query: 361 VEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESR 420
            EF+QLVEKAIKDSYAI+ KDST K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDES+
Sbjct: 361 DEFYQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQ 420

Query: 421 ESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHP 480
           ESL+KGLPV+SFSIGDSAEFL+GDRSD+DQAEKVTL+SGDILIFGGKSRHVFHGVT IH 
Sbjct: 421 ESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHS 480

Query: 481 NTAPKALLEETNLRSGRLNLTFRQY 483
            TAPKALLE TNLR GRLNLTFRQY
Sbjct: 481 KTAPKALLEATNLRPGRLNLTFRQY 501

[Close]

BLAST of MC03g0512 vs. ExPASy TrEMBL
Match: A0A6J1GV59 (uncharacterized protein LOC111457830 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457830 PE=4 SV=1)

HSP 1 Score: 677 bits (1747), Expect = 5.66e-241
Identity = 364/503 (72.37%), Postives = 401/503 (79.72%), Query Frame = 0


Query: 1   MNRGRPGYG-RPHPNSRGRSFRSADNYSYRPRHAGGEGPSLDGSSNPSSGDFRGRSSQQM 60
           MN G   YG R HPN+RGR  RSADN++YRPRHAGGEGPS  GSSNP +G F+ RSS   
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSH-- 60

Query: 61  PSRNFRKNYGQNQALSSEQWQWRPLNSGKDASPSPGAVDLQQEHSSADGAG--------- 120
           PSRN R  +GQ QAL SEQWQWRPLNSGKDASP  GAVDLQQ+H++AD            
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDASP--GAVDLQQDHNTADDIAPKQLIGSIS 120

Query: 121 ---------NASEQLLASTESNSNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCS 180
                    N SEQLL S  S S+ NE SP SA NVSK LHSAVERIQIREPT EGE C 
Sbjct: 121 SNSDGNDMSNTSEQLLGSIASKSDGNEHSPCSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 181 SLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKE-NNVSNCKGSEDNNKPSVNLD 240
              PYD+C R  A  QEL VQ        +++++IK +E NNVS+CK S+D  KP  NL+
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDK-KPLGNLE 240

Query: 241 PFDICPPKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQI 300
            FDICPPKSG +TLNPSLL+ N+EKRNE KR  EGN G VLRPGMV LKSGISL DQV+I
Sbjct: 241 IFDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKI 300

Query: 301 VKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVE 360
           VK+CR+LG+GAGGFYQPGYREGGKLHLKMMCLGKNWDPD+S YGDVRPFD+T PP +PVE
Sbjct: 301 VKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVE 360

Query: 361 FHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRES 420
           F++LVEKAIKDSYA+MGKDS  K PERVLPWM+PNICIVNFYSQNGRLGLHQDRDES+ES
Sbjct: 361 FYKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQES 420

Query: 421 LEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNT 480
           LEKGLPVVSFSIGDSAEFL+GD SDIDQAEKVTL+SGDILIFGGKSRHVFHGVT IH NT
Sbjct: 421 LEKGLPVVSFSIGDSAEFLFGDWSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNT 480

Query: 481 APKALLEETNLRSGRLNLTFRQY 483
           APKALLE TNLR GRLNLTFRQY
Sbjct: 481 APKALLEATNLRPGRLNLTFRQY 490

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BLAST of MC03g0512 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 345.5 bits (885), Expect = 6.9e-95
Identity = 178/317 (56.15%), Postives = 222/317 (70.03%), Query Frame = 0


Query: 167 DNCNRLAAVDQELKVQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICP 226
           D  NR+  V    +  V      K E SS++ +++          +N+ S     FDI  
Sbjct: 146 DLVNRVNDVTLSCQESVSSTVVQKVELSSVEDQKSAPKADGAGNSSNESSTR--HFDIFL 205

Query: 227 PKSGRITLNPSLLATNKEKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRN 286
            K G I L P+LL  ++EK    K+  +G  G V+RPGMVLLK+ +S+ DQV IV +CR 
Sbjct: 206 EKKG-IVLKPNLLVLSREK----KKAAKGYSGTVIRPGMVLLKNYLSINDQVMIVNKCRR 265

Query: 287 LGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVE 346
           LGLG GGFYQPGYR+  KLHLKMMCLGKNWDP+TS YG+ RPFD +  PRIP EF+Q VE
Sbjct: 266 LGLGEGGFYQPGYRDEAKLHLKMMCLGKNWDPETSRYGETRPFDGSTAPRIPAEFNQFVE 325

Query: 347 KAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDRDESRESLEKGLP 406
           KA+K+S ++   +S        +P+M P+ICIVNFYS  GRLGLHQD+DES  S+ KGLP
Sbjct: 326 KAVKESQSLAASNSKQTKGGDEIPFMLPDICIVNFYSSTGRLGLHQDKDESENSIRKGLP 385

Query: 407 VVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAPKALL 466
           VVSFSIGDSAEFLYGD+ D D+AE +TL+SGD+L+FGG+SR VFHGV  I  +TAPKALL
Sbjct: 386 VVSFSIGDSAEFLYGDQRDEDKAETLTLESGDVLLFGGRSRKVFHGVRSIRKDTAPKALL 445

Query: 467 EETNLRSGRLNLTFRQY 484
           +ET+LR GRLNLTFRQY
Sbjct: 446 QETSLRPGRLNLTFRQY 455

[Close]

BLAST of MC03g0512 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.4e-79
Identity = 157/329 (47.72%), Postives = 214/329 (65.05%), Query Frame = 0


Query: 159 SCSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVN 218
           S +S++ +D+ N  ++        + +       +S  KS++ +    K          +
Sbjct: 77  SPTSMVHFDSTNPSSSSKSSQSQNLKIRKVRNHRNSGFKSRDQSPQRIK----------D 136

Query: 219 LDPFDICPPKSGR--ITLNPSLLATNKEKRNETKRTT--EGNCGVVLRPGMVLLKSGISL 278
             PFDIC     R   ++   +LA      +ET R T    N   V+RPGMVLLK  ++ 
Sbjct: 137 PPPFDICSSVLERNDTSIKDWILA------DETNRETVEVSNKHKVIRPGMVLLKDFLTP 196

Query: 279 RDQVQIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKP 338
             QV IVK CR LG+   GFYQPGY  G KLHL+MMCLG+NWDP T    +     D+K 
Sbjct: 197 DIQVDIVKTCRELGVKPTGFYQPGYSVGSKLHLQMMCLGRNWDPQTKYRKNTD--IDSKA 256

Query: 339 PRIPVEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDR 398
           P IPV F+ LVEKAI++++A++ ++S  +  ER+LP M P+ICIVNFYS+ GRLGLHQDR
Sbjct: 257 PEIPVTFNVLVEKAIREAHALIDRESGTEDAERILPVMSPDICIVNFYSETGRLGLHQDR 316

Query: 399 DESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVT 458
           DES ES+ +GLP+VSFSIGDSAEFLYG++ D+++A+ V L+SGD+LIFGG+SR +FHGV 
Sbjct: 317 DESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVILESGDVLIFGGESRMIFHGVK 376

Query: 459 EIHPNTAPKALLEETNLRSGRLNLTFRQY 484
            I PN+AP +LL E+ LR+GRLNLTFR +
Sbjct: 377 SIIPNSAPMSLLNESKLRTGRLNLTFRHF 387

[Close]

BLAST of MC03g0512 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.4e-79
Identity = 157/329 (47.72%), Postives = 214/329 (65.05%), Query Frame = 0


Query: 159 SCSSLLPYDNCNRLAAVDQELKVQVPLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVN 218
           S +S++ +D+ N  ++        + +       +S  KS++ +    K          +
Sbjct: 132 SPTSMVHFDSTNPSSSSKSSQSQNLKIRKVRNHRNSGFKSRDQSPQRIK----------D 191

Query: 219 LDPFDICPPKSGR--ITLNPSLLATNKEKRNETKRTT--EGNCGVVLRPGMVLLKSGISL 278
             PFDIC     R   ++   +LA      +ET R T    N   V+RPGMVLLK  ++ 
Sbjct: 192 PPPFDICSSVLERNDTSIKDWILA------DETNRETVEVSNKHKVIRPGMVLLKDFLTP 251

Query: 279 RDQVQIVKRCRNLGLGAGGFYQPGYREGGKLHLKMMCLGKNWDPDTSTYGDVRPFDDTKP 338
             QV IVK CR LG+   GFYQPGY  G KLHL+MMCLG+NWDP T    +     D+K 
Sbjct: 252 DIQVDIVKTCRELGVKPTGFYQPGYSVGSKLHLQMMCLGRNWDPQTKYRKNTD--IDSKA 311

Query: 339 PRIPVEFHQLVEKAIKDSYAIMGKDSTAKTPERVLPWMEPNICIVNFYSQNGRLGLHQDR 398
           P IPV F+ LVEKAI++++A++ ++S  +  ER+LP M P+ICIVNFYS+ GRLGLHQDR
Sbjct: 312 PEIPVTFNVLVEKAIREAHALIDRESGTEDAERILPVMSPDICIVNFYSETGRLGLHQDR 371

Query: 399 DESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVT 458
           DES ES+ +GLP+VSFSIGDSAEFLYG++ D+++A+ V L+SGD+LIFGG+SR +FHGV 
Sbjct: 372 DESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVILESGDVLIFGGESRMIFHGVK 431

Query: 459 EIHPNTAPKALLEETNLRSGRLNLTFRQY 484
            I PN+AP +LL E+ LR+GRLNLTFR +
Sbjct: 432 SIIPNSAPMSLLNESKLRTGRLNLTFRHF 442

[Close]

BLAST of MC03g0512 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-74
Identity = 157/361 (43.49%), Postives = 217/361 (60.11%), Query Frame = 0


Query: 124 SNSNELSPSSALNVSKDLHSAVERIQIREPTTEGESCSSLLPYDNCNRLAAVDQELKVQV 183
           S  N+    S L+ SK+++ +      RE   +     + +  D  NR+  V       V
Sbjct: 108 SGDNKDILQSTLSSSKNINLSGGLFVSREYENKYVKFGARMRCDLVNRMKDVTLAWHESV 167

Query: 184 PLESCAKEESSSIKSKENNVSNCKGSEDNNKPSVNLDPFDICPPKSGRITLNPSLLATNK 243
              S    E SS+++  + ++       N+    +  PFDI   K   + L PS L  N+
Sbjct: 168 SSTSDKNVELSSVEN--HKIAPKADGPGNSSNESSSSPFDIFLKKK-VMRLKPSFLELNR 227

Query: 244 EKRNETKRTTEGNCGVVLRPGMVLLKSGISLRDQVQIVKRCRNLGLGAGGFYQPGYREGG 303
           EK    K+  +G  G+V+RPGMVLLK+ +S+ +QV IV +CR LGLG GGFYQPG+++GG
Sbjct: 228 EK----KKAAKGFSGIVIRPGMVLLKNYLSINNQVMIVNKCRQLGLGEGGFYQPGFQDGG 287

Query: 304 KLHLKMMCLGKNWDPDTSTYGDVRPFDDTKPPRIPVEFHQLVEKAIKDSYAIMGKDSTAK 363
            LHLKMMCLGKNWD  T  YG++RP D + PPRIPVEF QLVEKAIK+S +++  +S   
Sbjct: 288 LLHLKMMCLGKNWDCQTRRYGEIRPIDGSVPPRIPVEFSQLVEKAIKESKSLVATNSNET 347

Query: 364 TPERVLPWMEPNICIVNFYSQNGRLGLHQ---------------------DRDESRESLE 423
                +P + P+IC+VNFY+  G+LGLHQ                     D+ ES++SL 
Sbjct: 348 KGGDEIPLLLPDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLR 407

Query: 424 KGLPVVSFSIGDSAEFLYGDRSDIDQAEKVTLKSGDILIFGGKSRHVFHGVTEIHPNTAP 464
           KGLP+VSFSIGDSAEFLYGD+ D+D+A+ + L+SGD+LIFG +SR+VFHGV  I     P
Sbjct: 408 KGLPIVSFSIGDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSIRKILPP 461

[Close]

BLAST of MC03g0512 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 2.6e-09
Identity = 40/122 (32.79%), Postives = 58/122 (47.54%), Query Frame = 0


Query: 374 PNICIVNFYSQNGRLGLHQDRDESRESLEKGLPVVSFSIGDSAEFLYGDRSDIDQAEKVT 433
           P   IVN++     LG H D  E+    +   P+VS S+G  A FL G +S  D    + 
Sbjct: 226 PEGAIVNYFGIGDTLGGHLDDMEA----DWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMY 285

Query: 434 LKSGDILIFGGKSRHVFHGVTEI---HPNTAPKALLEETNLRSG----------RLNLTF 483
           L+SGD+++  G++R  FHG+  I     N    AL  E +  SG          R+N+  
Sbjct: 286 LRSGDVVLMAGEARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININI 343

The following BLAST results are available for this feature:

BLAST of MC03g0512 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0CAT7	2.0e-14	30.61	Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides...	[more]
B8GWW6	2.0e-14	30.61	Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides...	[more]
P05050	5.8e-14	30.00	Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Escherichia coli (strain K12) ...	[more]
P37462	2.9e-13	30.11	Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Salmonella typhimurium (strain...	[more]
Q54N08	3.0e-10	36.96	Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44...	[more]

BLAST of MC03g0512 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142665.1	0.0	100.00	uncharacterized protein LOC111012720 [Momordica charantia]	[more]
XP_038875730.1	1.58e-255	75.65	uncharacterized protein LOC120068103 isoform X1 [Benincasa hispida]	[more]
KAA0058530.1	3.01e-246	73.07	Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa] >TYK07216.1 O...	[more]
XP_016903133.1	5.54e-244	72.87	PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 [Cucumis me...	[more]
KAG6581809.1	1.43e-241	72.56	hypothetical protein SDJN03_21811, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MC03g0512 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLK3	0.0	100.00	uncharacterized protein LOC111012720 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A0A0LC72	1.72e-248	75.05	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5D3CA69	1.46e-246	73.07	Oxoglutarate/iron-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S4E4H2	2.68e-244	72.87	LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 OS=Cucumis melo OX=365...	[more]
A0A6J1GV59	5.66e-241	72.37	uncharacterized protein LOC111457830 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MC03g0512 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14160.1	6.9e-95	56.15	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.1	2.4e-79	47.72	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT5G01780.2	2.4e-79	47.72	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT3G14140.1	1.0e-74	43.49	2-oxoglutarate-dependent dioxygenase family protein	[more]
AT1G11780.1	2.6e-09	32.79	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 264..481 e-value: 4.5E-42 score: 144.5
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 259..483 e-value: 8.0E-52 score: 177.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..111
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..87
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 97..111
None	No IPR available	PANTHER	PTHR16557:SF9	2OG-FE(II) OXYGENASE FAMILY PROTEIN	coord: 106..483
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 261..483
IPR004574	Alkylated DNA repair protein AlkB	PANTHER	PTHR16557	ALKYLATED DNA REPAIR PROTEIN ALKB-RELATED	coord: 106..483
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 373..483 score: 10.377964

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0512.1	MC03g0512.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
molecular_function	GO:0051213	dioxygenase activity