MC03g0448 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0448
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	MC03: 11364009 .. 11364752 (+)
RNA-Seq Expression	MC03g0448
Synteny	MC03g0448

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACTAGAAGGCAAAGTGGCAATAATCACTGGTGGGGCCAGAGGAATCGGGGAGTACACTGCAAGGCTCTTCTGCAAGCATGGAGCCAAAGTGGTGATTGCAGACATCAATGACGATCTAGGTCAATCCCTCTCCAACCATCTTGGCTCTTCATCTTCTTCCTTCGTCCATTGCGATGTAACCAAAGAAAAAGATGTCGAAAACATCGTCGACACAACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGCCATTCTTGGAGCCCCAAACTACAACATTCTTGAAAACGACTCATCAGACTTCCAGAAGGTACTCAATGTCAACCTTTTCGGTGCCTTCCTCGGCACGAAACACGCGGCGCGGGCGATGATTCCGGCTCGTCGAGGTAGCATAATCACAGCTGCAAGTGCTGCGGCGACCGTGGGCGGGATTGCCCCCCATGCATGCACAAGCTCAAAGCATGGAGTTTTGGGATTGATGAAGAATGCAGCTGTGGATTTAGGACGGTATGGGATTAGAGTGAATTGTGTGTCGCCCTATGCAGTGCCCACACAATTGACCAGGGACTTGCTTAGGCTCAAAGATGGGGACAAGTTTCCTAATATGTATTGGAATCTGAAGAATGAAGATGCTATAAGTGAAGAAGATGTGGCTGAAGCAGCTCTTTATCTGGGGAGTGATGCGGCTAAGTACGTGAGTGGGCACAATTTAGTTGTTGATGGAGGCTTCTCT

mRNA sequence

AGACTAGAAGGCAAAGTGGCAATAATCACTGGTGGGGCCAGAGGAATCGGGGAGTACACTGCAAGGCTCTTCTGCAAGCATGGAGCCAAAGTGGTGATTGCAGACATCAATGACGATCTAGGTCAATCCCTCTCCAACCATCTTGGCTCTTCATCTTCTTCCTTCGTCCATTGCGATGTAACCAAAGAAAAAGATGTCGAAAACATCGTCGACACAACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGCCATTCTTGGAGCCCCAAACTACAACATTCTTGAAAACGACTCATCAGACTTCCAGAAGGTACTCAATGTCAACCTTTTCGGTGCCTTCCTCGGCACGAAACACGCGGCGCGGGCGATGATTCCGGCTCGTCGAGGTAGCATAATCACAGCTGCAAGTGCTGCGGCGACCGTGGGCGGGATTGCCCCCCATGCATGCACAAGCTCAAAGCATGGAGTTTTGGGATTGATGAAGAATGCAGCTGTGGATTTAGGACGGTATGGGATTAGAGTGAATTGTGTGTCGCCCTATGCAGTGCCCACACAATTGACCAGGGACTTGCTTAGGCTCAAAGATGGGGACAAGTTTCCTAATATGTATTGGAATCTGAAGAATGAAGATGCTATAAGTGAAGAAGATGTGGCTGAAGCAGCTCTTTATCTGGGGAGTGATGCGGCTAAGTACGTGAGTGGGCACAATTTAGTTGTTGATGGAGGCTTCTCT

Coding sequence (CDS)

AGACTAGAAGGCAAAGTGGCAATAATCACTGGTGGGGCCAGAGGAATCGGGGAGTACACTGCAAGGCTCTTCTGCAAGCATGGAGCCAAAGTGGTGATTGCAGACATCAATGACGATCTAGGTCAATCCCTCTCCAACCATCTTGGCTCTTCATCTTCTTCCTTCGTCCATTGCGATGTAACCAAAGAAAAAGATGTCGAAAACATCGTCGACACAACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAACGCAGCCATTCTTGGAGCCCCAAACTACAACATTCTTGAAAACGACTCATCAGACTTCCAGAAGGTACTCAATGTCAACCTTTTCGGTGCCTTCCTCGGCACGAAACACGCGGCGCGGGCGATGATTCCGGCTCGTCGAGGTAGCATAATCACAGCTGCAAGTGCTGCGGCGACCGTGGGCGGGATTGCCCCCCATGCATGCACAAGCTCAAAGCATGGAGTTTTGGGATTGATGAAGAATGCAGCTGTGGATTTAGGACGGTATGGGATTAGAGTGAATTGTGTGTCGCCCTATGCAGTGCCCACACAATTGACCAGGGACTTGCTTAGGCTCAAAGATGGGGACAAGTTTCCTAATATGTATTGGAATCTGAAGAATGAAGATGCTATAAGTGAAGAAGATGTGGCTGAAGCAGCTCTTTATCTGGGGAGTGATGCGGCTAAGTACGTGAGTGGGCACAATTTAGTTGTTGATGGAGGCTTCTCT

Protein sequence

RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDVTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGTKHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNCVSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHNLVVDGGFS

Homology

[Close]

BLAST of MC03g0448 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 4.4e-74
Identity = 143/251 (56.97%), Postives = 186/251 (74.10%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGA GIGE TA+LF +HGAKV IAD+ D+LG S+   +G+S+S+++HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E  V+N VD TVS YGKLDIMF+NA I       I++N+ +DF++V +VN+ G FL  
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCM 133

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAAR MIPAR G+II+ AS ++T+GG + HA   SKH VLGL +N AV+LG++GIRVNC
Sbjct: 134 KHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNC 193

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMY---WNLKNEDAISEEDVAEAALYLGSDAAKYVS 240
           +SP+ +PT L +    +K+ ++F N+     NLK     + EDVA AALYL SD AKYVS
Sbjct: 194 LSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKG-PKFNVEDVANAALYLASDEAKYVS 253

Query: 241 GHNLVVDGGFS 249
           GHNL +DGGFS
Sbjct: 254 GHNLFIDGGFS 263

[Close]

BLAST of MC03g0448 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.2e-70
Identity = 141/250 (56.40%), Postives = 175/250 (70.00%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           +L GKVA+ITGGA GIG  TARLF KHGA+VV+ADI D+LG SL   LG  +SS+VHCDV
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDV 73

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E DV   VD  V+++GKLD+MFNNA + G P + + E    DF++VL VNL G FLGT
Sbjct: 74  TNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLGT 133

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAAR M PARRGSII+ AS +++V G A HA T+SKH ++G  +NAA +LGR+GIRVNC
Sbjct: 134 KHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRVNC 193

Query: 181 VSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSG 240
           VSP  V T L R  + + D   +       NLK   A+  +D+A AAL+L SD  +YVSG
Sbjct: 194 VSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYVSG 253

Query: 241 HNLVVDGGFS 249
            NL VDGG S
Sbjct: 254 QNLRVDGGLS 263

[Close]

BLAST of MC03g0448 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 4.0e-67
Identity = 135/252 (53.57%), Postives = 180/252 (71.43%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSS-SSFVHCD 60
           RL+ KVAIITGGA GIGE TA+LF ++GAKVVIADI DD GQ + N++GS    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLG 120
           VTK++DV N+VDTT++K+GKLDIMF N  +L    Y+ILE  + DF++V+++N++GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMIPARRGSII-TAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRV 180
            KHAAR MIPA++GSI+ TA+ ++ T G    H  T++KH VLGL  +   +LG YGIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 181 NCVSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYV 240
           NCVSPY V + L  D+  +     ++  +   NLK    +  EDVA+A  YL  D +KYV
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKG-TLLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLVVDGGFS 249
           SG NLV+DGG++
Sbjct: 253 SGLNLVIDGGYT 263

[Close]

BLAST of MC03g0448 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.7e-67
Identity = 134/252 (53.17%), Postives = 182/252 (72.22%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSS-SSFVHCD 60
           RL+ KVAIITGGA GIGE TA+LF ++GAKVVIADI+DD GQ + N++GS    SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLG 120
           VTK++DV N+VDTT++K+GKLDIMF N  +L    Y+ILE  + DF++V+++N++GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMIPARRGSII-TAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRV 180
            KHAAR MIPA++GSI+ TA+ ++ T G    H  T++KH VLGL  +   +LG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 181 NCVSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYV 240
           NCVSPY V + L  D+  +     ++  +   NLK    +  EDVA+A  YL  D +KYV
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKG-ILLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLVVDGGFS 249
           SG NLV+DGG++
Sbjct: 253 SGLNLVIDGGYT 263

[Close]

BLAST of MC03g0448 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 7.5e-66
Identity = 134/257 (52.14%), Postives = 171/257 (66.54%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSS-SSSFVHCD 60
           RLEGKVA++TGGA GIGE  ARLF +HGAK+ I D+ D+LGQ +S  LG    + + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 61  VTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLG 120
           VT E DV   VD T  KYG +DIM NNA I G    +I + D ++F+KV ++N+ G FLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 121 TKHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
            KHAAR MIP  +GSI++ AS ++ + G  PH  T +KH V+GL K+ A +LGR+GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 181 CVSPYAVPTQLTRDLL--------RLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSD 240
           CVSPYAVPT+L+   L         L+    F     NLK  D +   DVAEA LYL ++
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVD-LMPNDVAEAVLYLATE 241

Query: 241 AAKYVSGHNLVVDGGFS 249
            +KYVSG NLV+DGGFS
Sbjct: 242 ESKYVSGLNLVIDGGFS 257

[Close]

BLAST of MC03g0448 vs. NCBI nr
Match: XP_022142634.1 (secoisolariciresinol dehydrogenase-like [Momordica charantia])

HSP 1 Score: 487 bits (1254), Expect = 3.88e-173
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV
Sbjct: 12  RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT
Sbjct: 72  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN
Sbjct: 192 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 251

Query: 241 LVVDGGFS 248
           LVVDGGFS
Sbjct: 252 LVVDGGFS 259

[Close]

BLAST of MC03g0448 vs. NCBI nr
Match: XP_038895162.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 407 bits (1046), Expect = 1.98e-141
Identity = 203/249 (81.53%), Postives = 224/249 (89.96%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGEYTARLF KHGAKVVIADI DDLG SLSNH+GSSS SFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIEDDLGHSLSNHIGSSSFSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN VD+ +S YGKLDIMFNNAAILGAP +NILEN+SSDFQKVLNVNL GAFLGT
Sbjct: 72  TKEKDVENAVDSALSDYGKLDIMFNNAAILGAPKFNILENESSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAAR MIPA RGSIIT  S  +++GGI PHACTSSKHGV+GLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARVMIPAGRGSIITTGSVISSIGGIGPHACTSSKHGVVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLK-NEDAISEEDVAEAALYLGSDAAKYVSGH 240
           VSPY V T++TR+L ++KDGD+ P++YWNLK  ED + EEDVAEAALYL SDA+KYVSGH
Sbjct: 192 VSPYGVHTKMTRELFKVKDGDEIPSLYWNLKCGEDVVKEEDVAEAALYLASDASKYVSGH 251

Query: 241 NLVVDGGFS 248
           NLVVDGGF+
Sbjct: 252 NLVVDGGFT 260

[Close]

BLAST of MC03g0448 vs. NCBI nr
Match: XP_022142407.1 (momilactone A synthase-like [Momordica charantia])

HSP 1 Score: 383 bits (983), Expect = 8.60e-132
Identity = 194/248 (78.23%), Postives = 216/248 (87.10%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGEYTARLF KHGAKVVIADI DDLG SLSN+L  SSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T+E DV N VDTTVSKYGKLDIMFNNA I G   +NILEN+S DFQKVLNVNL GAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAM+PARRGSII  AS  + +GG  PHA TSSKHG++GLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VPTQ+TRDL +L+DGD+FPN Y +LK    + EED+AEAALYLGSDA+K+VSGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LVVDGGFS 248
           L+VDGGF+
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0448 vs. NCBI nr
Match: XP_022927563.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 369 bits (947), Expect = 2.59e-126
Identity = 185/248 (74.60%), Postives = 212/248 (85.48%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN V+T +SKYGKLDIM NNA ILG P +NILEN+SSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+ +TR+L +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSDITRELFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFS 248
           L+VDGGF+
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0448 vs. NCBI nr
Match: XP_023520696.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369 bits (947), Expect = 2.59e-126
Identity = 184/248 (74.19%), Postives = 211/248 (85.08%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADIQDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN V+T +SKYGKLDIM NNA ILG P +NILENDSSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENDSSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+ +T++L +L+DGD+FPN+Y + K  D +  ED+AE ALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSDMTKELFKLEDGDEFPNVYSHFKCGDILRAEDIAETALYLASDASRFVSGHN 251

Query: 241 LVVDGGFS 248
           L+VDGGF+
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0448 vs. ExPASy TrEMBL
Match: A0A6J1CM16 (secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111012697 PE=4 SV=1)

HSP 1 Score: 487 bits (1254), Expect = 1.88e-173
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV
Sbjct: 12  RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT
Sbjct: 72  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN
Sbjct: 192 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 251

Query: 241 LVVDGGFS 248
           LVVDGGFS
Sbjct: 252 LVVDGGFS 259

[Close]

BLAST of MC03g0448 vs. ExPASy TrEMBL
Match: A0A6J1CMN7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 SV=1)

HSP 1 Score: 383 bits (983), Expect = 4.16e-132
Identity = 194/248 (78.23%), Postives = 216/248 (87.10%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITGGARGIGEYTARLF KHGAKVVIADI DDLG SLSN+L  SSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T+E DV N VDTTVSKYGKLDIMFNNA I G   +NILEN+S DFQKVLNVNL GAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAM+PARRGSII  AS  + +GG  PHA TSSKHG++GLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VPTQ+TRDL +L+DGD+FPN Y +LK    + EED+AEAALYLGSDA+K+VSGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LVVDGGFS 248
           L+VDGGF+
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0448 vs. ExPASy TrEMBL
Match: A0A6J1ELC4 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111434357 PE=4 SV=1)

HSP 1 Score: 369 bits (947), Expect = 1.25e-126
Identity = 185/248 (74.60%), Postives = 212/248 (85.48%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKDVEN V+T +SKYGKLDIM NNA ILG P +NILEN+SSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKDVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSAIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+ +TR+L +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSDITRELFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFS 248
           L+VDGGF+
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0448 vs. ExPASy TrEMBL
Match: A0A6J1I7X4 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471669 PE=4 SV=1)

HSP 1 Score: 368 bits (945), Expect = 2.53e-126
Identity = 184/247 (74.49%), Postives = 211/247 (85.43%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+I G ARGIGE TAR F KHGAK+VIADI DD+G SLSNHL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVILGAARGIGESTARTFFKHGAKLVIADILDDVGHSLSNHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEK+VEN V+T +SKYGKLDIM NNA ILG P +NILEN+SSDFQKVLNVNL G FLGT
Sbjct: 72  TKEKEVENAVNTAISKYGKLDIMLNNAGILGNPIFNILENESSDFQKVLNVNLLGTFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAARAMIPAR+GSII  AS  + +GGI P+  TSSKHGVLGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+Q+T+DL +L+DGD+FPN Y + K  D +  +D+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSQMTKDLFKLEDGDEFPNFYSHFKYGDILRADDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGF 247
           L+VDGGF
Sbjct: 252 LIVDGGF 258

[Close]

BLAST of MC03g0448 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 367 bits (941), Expect = 8.92e-126
Identity = 183/248 (73.79%), Postives = 213/248 (85.89%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LF KHGAKVV+ADI DD+G SLS HL SSSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           TKEKD+EN V+T +SKYGKLDIM NNA I G   +NILEN+ SDFQKVLNVNL GAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVNC 180
           KHAA+AMIPAR+GSII  AS  + +GGI P+A TSSKHG+LGLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSGHN 240
           VSP+ VP+Q+TRDL +L+DGD+FPN+Y + K  D +  ED+AEAALYL SDA+++VSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LVVDGGFS 248
           L+VDGGF+
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0448 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.3e-65
Identity = 136/264 (51.52%), Postives = 179/264 (67.80%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADIND----DLGQSLSNHLGSSSSSFV 60
           RLEGKVAIITGGA GIG+ T  LF +HGA VVIAD+++     L +SLS+H  S   +F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTKEKDVENIVDTTVSKYGKLDIMFNNAAILG--APNYNILENDSSDFQKVLNVNLF 120
            CDV+ E DVEN+V+ TV++YG+LDI+FNNA +LG    + +IL+ D+ +F  V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GAFLGTKHAARAMIP-ARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGR 180
           G  LG KH ARAMI    +G II+ AS A  +GG+ PHA T+SKH ++GL KNAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 YGIRVNCVSPYAVPTQLTRDLLRLKDG-----------DKFPNMYWNLKNEDAISEEDVA 240
           YGIRVNC+SP+ V T +  +  R   G           ++F     NLK E  +   D+A
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLKGE-TLRANDIA 270

Query: 241 EAALYLGSDAAKYVSGHNLVVDGG 247
           EAALYL SD +KYV+GHNLVVDGG
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGG 293

[Close]

BLAST of MC03g0448 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 6.3e-60
Identity = 123/261 (47.13%), Postives = 166/261 (63.60%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLG----QSLSNHLGSSSSSFV 60
           RL GKVA+ITGGA GIGE   RLF KHGAKV I D+ DDLG    +SL       ++ F+
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 61  HCDVTKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGA 120
           H DV  E D+ N VD  V  +G LDI+ NNA + GAP  +I     S+F+   +VN+ GA
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 121 FLGTKHAARAMIPARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGI 180
           FL  KHAAR MIP ++GSI++  S    VGG+ PH+   SKH VLGL ++ A +LG++GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 181 RVNCVSPYAVPTQLTRDLLRLKDGDKFPNMYWNLKNEDA---------ISEEDVAEAALY 240
           RVNCVSPYAV T+L   L  L + ++  + +   +N  A         ++ +DVA A L+
Sbjct: 197 RVNCVSPYAVATKLA--LAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLF 256

Query: 241 LGSDAAKYVSGHNLVVDGGFS 249
           L SD ++Y+SG NL++DGGF+
Sbjct: 257 LASDDSRYISGDNLMIDGGFT 275

[Close]

BLAST of MC03g0448 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.0e-58
Identity = 124/250 (49.60%), Postives = 166/250 (66.40%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RL+GK+AIITGGA GIG    RLF  HGAKVVI D  ++LGQ+++  +G   +SF  CDV
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T EK+VEN V  TV KYGKLD++F+NA ++  P  + L+ +   F + + VN+ GA    
Sbjct: 65  TNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQPG-SFLDLNLEQFDRTMAVNVRGAAAFI 124

Query: 121 KHAARAMI-PARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+   S A+ +GG  PHA T+SKH +LGL+K+A   LG+YGIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRVN 184

Query: 181 CVSPYAVPTQL-TRDLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVSG 240
            V+PYAV T + +RD   ++  +++      LK    +    VAEAAL+L SD + YVSG
Sbjct: 185 GVAPYAVATAINSRDEETVRMVEEYSAATGILKGV-VLKARHVAEAALFLASDDSAYVSG 244

Query: 241 HNLVVDGGFS 249
            NL VDGG+S
Sbjct: 245 QNLAVDGGYS 252

[Close]

BLAST of MC03g0448 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 3.4e-58
Identity = 120/251 (47.81%), Postives = 169/251 (67.33%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RL+GK+ IITGGA GIG  + RLF +HGA+VVI D+ D+LGQ+++  +G   +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E +VEN V  TV KYGKLD++F+NA ++  P  +IL+ + ++  + + +NL G     
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVI-EPFVSILDLNLNELDRTIAINLRGTAAFI 124

Query: 121 KHAARAMI-PARRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+   S AA + G APH  T+SKHG+LGL+K+A+  LG+YGIRVN
Sbjct: 125 KHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRVN 184

Query: 181 CVSPYAVPTQLTRDLLRLKDG--DKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVS 240
            V+P+ V T L  +  +++    ++  +   NLK    +    VAEAAL+L SD + YVS
Sbjct: 185 GVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKG-IVLKARHVAEAALFLASDESAYVS 244

Query: 241 GHNLVVDGGFS 249
           G NL VDGG+S
Sbjct: 245 GQNLAVDGGYS 253

[Close]

BLAST of MC03g0448 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 7.7e-58
Identity = 124/251 (49.40%), Postives = 168/251 (66.93%), Query Frame = 0


Query: 1   RLEGKVAIITGGARGIGEYTARLFCKHGAKVVIADINDDLGQSLSNHLGSSSSSFVHCDV 60
           RL+GK+AIITGGA GIG    RLF  HGAKVVI DI ++LGQ+L+  +G   +SF  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 61  TKEKDVENIVDTTVSKYGKLDIMFNNAAILGAPNYNILENDSSDFQKVLNVNLFGAFLGT 120
           T E DVEN V  TV K+GKLD++F+NA +L A   ++L+ D   F + + VN+ GA    
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFG-SVLDLDLEAFDRTMAVNVRGAAAFI 124

Query: 121 KHAARAMIPA-RRGSIITAASAAATVGGIAPHACTSSKHGVLGLMKNAAVDLGRYGIRVN 180
           KHAAR+M+ +  RGSI+   S AA +GG  PH+ T+SKH +LGL+++A   LG+YGIRVN
Sbjct: 125 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 184

Query: 181 CVSPYAVPTQLTR--DLLRLKDGDKFPNMYWNLKNEDAISEEDVAEAALYLGSDAAKYVS 240
            V+PY V T +T   +   +K  +++     NLK    +    +AEAAL+L SD + Y+S
Sbjct: 185 GVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGV-VLKARHIAEAALFLASDDSVYIS 244

Query: 241 GHNLVVDGGFS 249
           G NLVVDGGFS
Sbjct: 245 GQNLVVDGGFS 253

The following BLAST results are available for this feature:

BLAST of MC03g0448 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	4.4e-74	56.97	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	2.2e-70	56.40	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q94KL8	4.0e-67	53.57	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	6.7e-67	53.17	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
F1SWA0	7.5e-66	52.14	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of MC03g0448 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142634.1	3.88e-173	100.00	secoisolariciresinol dehydrogenase-like [Momordica charantia]	[more]
XP_038895162.1	1.98e-141	81.53	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]
XP_022142407.1	8.60e-132	78.23	momilactone A synthase-like [Momordica charantia]	[more]
XP_022927563.1	2.59e-126	74.60	secoisolariciresinol dehydrogenase-like [Cucurbita moschata]	[more]
XP_023520696.1	2.59e-126	74.19	secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC03g0448 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CM16	1.88e-173	100.00	secoisolariciresinol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1CMN7	4.16e-132	78.23	momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 ...	[more]
A0A6J1ELC4	1.25e-126	74.60	secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I7X4	2.53e-126	74.49	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]
A0A6J1EID0	8.92e-126	73.79	secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]

BLAST of MC03g0448 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	1.3e-65	51.52	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	6.3e-60	47.13	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	2.0e-58	49.60	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	3.4e-58	47.81	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	7.7e-58	49.40	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 153..172 score: 31.98 coord: 211..231 score: 25.28 coord: 174..191 score: 40.89 coord: 6..23 score: 48.82 coord: 127..143 score: 32.25 coord: 78..89 score: 51.35
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 11..248 e-value: 1.4E-63 score: 214.6
None	No IPR available	GENE3D	3.40.50.720		coord: 1..248 e-value: 6.5E-75 score: 253.6
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 2..248 e-value: 6.8E-19 score: 65.8
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..248
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 1..248
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..247

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0448.1	MC03g0448.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity