MC03g0447 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0447
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	MC03: 11354004 .. 11354747 (+)
RNA-Seq Expression	MC03g0447
Synteny	MC03g0447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGACTAGAAGGCAAAGTGGCACTAATCACGGGTGGGGCTAGAGGAATTGGAGAATACACTGCAAGACTGTTCTTCAAGCATGGAGCTAAAGTGGTCATTGCTGACATCCAAGACGATTTAGGTCACTCTCTTTCCAACAATCTCTGCCCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAGAGAGAACGACGTCGCAAATGCTGTCGATACGACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAATGCAGGTATTTCTGGAGACCTAAAGTTCAACATTCTCGAAAACGAATCATTAGACTTTCAAAAGGTACTCAATGTCAACCTTCTCGGAGCCTTTCTCGGCACAAAACACGCGGCACGAGCAATGATGCCAGCTCGTCGGGGCAGCATAATCATGACCGCAAGCGGTTGCTCGGTCATTGGCGGGGCGTGCCCGCATGCATACACGAGCTCAAAACATGGAATGGTGGGATTGATGAAGAATGCAGCTGTGGATTTGGGACGATATGGGATTAGGGTGAATTGTGTATCGCCCCATGTAGTGCCCACACAAATGACTAGGGACTTGTTTAAGCTTGAAGATGGTGATGAGTTTCCAAATCATTATTCTCATTTGAAATGTGGAGGGATGTTAATGGAAGAAGATATTGCAGAAGCTGCTTTGTATTTAGGAAGTGATGCGTCTAAGTTTGTGAGTGGCCATAATTTAATTGTTGATGGAGGCTTCACT

mRNA sequence

AGACTAGAAGGCAAAGTGGCACTAATCACGGGTGGGGCTAGAGGAATTGGAGAATACACTGCAAGACTGTTCTTCAAGCATGGAGCTAAAGTGGTCATTGCTGACATCCAAGACGATTTAGGTCACTCTCTTTCCAACAATCTCTGCCCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAGAGAGAACGACGTCGCAAATGCTGTCGATACGACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAATGCAGGTATTTCTGGAGACCTAAAGTTCAACATTCTCGAAAACGAATCATTAGACTTTCAAAAGGTACTCAATGTCAACCTTCTCGGAGCCTTTCTCGGCACAAAACACGCGGCACGAGCAATGATGCCAGCTCGTCGGGGCAGCATAATCATGACCGCAAGCGGTTGCTCGGTCATTGGCGGGGCGTGCCCGCATGCATACACGAGCTCAAAACATGGAATGGTGGGATTGATGAAGAATGCAGCTGTGGATTTGGGACGATATGGGATTAGGGTGAATTGTGTATCGCCCCATGTAGTGCCCACACAAATGACTAGGGACTTGTTTAAGCTTGAAGATGGTGATGAGTTTCCAAATCATTATTCTCATTTGAAATGTGGAGGGATGTTAATGGAAGAAGATATTGCAGAAGCTGCTTTGTATTTAGGAAGTGATGCGTCTAAGTTTGTGAGTGGCCATAATTTAATTGTTGATGGAGGCTTCACT

Coding sequence (CDS)

AGACTAGAAGGCAAAGTGGCACTAATCACGGGTGGGGCTAGAGGAATTGGAGAATACACTGCAAGACTGTTCTTCAAGCATGGAGCTAAAGTGGTCATTGCTGACATCCAAGACGATTTAGGTCACTCTCTTTCCAACAATCTCTGCCCTTCATCTTCTTCCTTCGTCCACTGCGACGTAACGAGAGAGAACGACGTCGCAAATGCTGTCGATACGACCGTCTCCAAGTACGGAAAGCTCGACATCATGTTCAACAATGCAGGTATTTCTGGAGACCTAAAGTTCAACATTCTCGAAAACGAATCATTAGACTTTCAAAAGGTACTCAATGTCAACCTTCTCGGAGCCTTTCTCGGCACAAAACACGCGGCACGAGCAATGATGCCAGCTCGTCGGGGCAGCATAATCATGACCGCAAGCGGTTGCTCGGTCATTGGCGGGGCGTGCCCGCATGCATACACGAGCTCAAAACATGGAATGGTGGGATTGATGAAGAATGCAGCTGTGGATTTGGGACGATATGGGATTAGGGTGAATTGTGTATCGCCCCATGTAGTGCCCACACAAATGACTAGGGACTTGTTTAAGCTTGAAGATGGTGATGAGTTTCCAAATCATTATTCTCATTTGAAATGTGGAGGGATGTTAATGGAAGAAGATATTGCAGAAGCTGCTTTGTATTTAGGAAGTGATGCGTCTAAGTTTGTGAGTGGCCATAATTTAATTGTTGATGGAGGCTTCACT

Protein sequence

RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDVTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGTKHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNCVSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHNLIVDGGFT

Homology

[Close]

BLAST of MC03g0447 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.0e-75
Identity = 141/250 (56.40%), Postives = 185/250 (74.00%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVALITGGA GIGE TA+LF +HGAKV IAD+QD+LGHS+   +  S+S+++HCDV
Sbjct: 14  RLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCDV 73

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T E+ V NAVD TVS YGKLDIMF+NAGIS   +  I++NE  DF++V +VN+ G FL  
Sbjct: 74  TNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERVFSVNVTGVFLCM 133

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAAR M+PAR G+II TAS  S +GG   HAY  SKH ++GL +N AV+LG++GIRVNC
Sbjct: 134 KHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGIRVNC 193

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPN--HYSHLKCGGMLMEEDIAEAALYLGSDASKFVSG 240
           +SP  +PT + +    +++ +EF N  +++    G     ED+A AALYL SD +K+VSG
Sbjct: 194 LSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVSG 253

Query: 241 HNLIVDGGFT 249
           HNL +DGGF+
Sbjct: 254 HNLFIDGGFS 263

[Close]

BLAST of MC03g0447 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 3.3e-74
Identity = 147/250 (58.80%), Postives = 181/250 (72.40%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           +L GKVA+ITGGA GIG  TARLF KHGA+VV+ADIQD+LG SL   L P +SS+VHCDV
Sbjct: 14  KLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSYVHCDV 73

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T E DVA AVD  V+++GKLD+MFNNAG+SG   F + E    DF++VL VNL+G FLGT
Sbjct: 74  TNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFLGT 133

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAAR M PARRGSII TAS  S + GA  HAYT+SKH +VG  +NAA +LGR+GIRVNC
Sbjct: 134 KHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRVNC 193

Query: 181 VSPHVVPTQMTRDLFKLEDG--DEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSG 240
           VSP  V T + R    ++D   +    + ++LK  G L  +DIA AAL+L SD  ++VSG
Sbjct: 194 VSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYVSG 253

Query: 241 HNLIVDGGFT 249
            NL VDGG +
Sbjct: 254 QNLRVDGGLS 263

[Close]

BLAST of MC03g0447 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 4.7e-68
Identity = 130/252 (51.59%), Postives = 180/252 (71.43%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNL-CPSSSSFVHCD 60
           RL+ KVA+ITGGA GIGE TA+LF ++GAKVVIADI DD G  + NN+  P   SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLG 120
           VT++ DV N VDTT++K+GKLDIMF N G+     ++ILE  + DF++V+++N+ GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMMPARRGSIIMTASGCSVIGG-ACPHAYTSSKHGMVGLMKNAAVDLGRYGIRV 180
            KHAAR M+PA++GSI+ TAS  S   G    H YT++KH ++GL  +   +LG YGIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 181 NCVSPHVVPTQMTRDLFKLEDG--DEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFV 240
           NCVSP++V + +  D+F ++    +E  +  ++LK G +L  ED+A+A  YL  D SK+V
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLK-GTLLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLIVDGGFT 249
           SG NL++DGG+T
Sbjct: 253 SGLNLVIDGGYT 263

[Close]

BLAST of MC03g0447 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.4e-67
Identity = 130/252 (51.59%), Postives = 181/252 (71.83%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNL-CPSSSSFVHCD 60
           RL+ KVA+ITGGA GIGE TA+LF ++GAKVVIADI DD G  + NN+  P   SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 61  VTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLG 120
           VT++ DV N VDTT++K+GKLDIMF N G+     ++ILE  + DF++V+++N+ GAFL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 121 TKHAARAMMPARRGSIIMTASGCSVIGG-ACPHAYTSSKHGMVGLMKNAAVDLGRYGIRV 180
            KHAAR M+PA++GSI+ TAS  S   G    H YT++KH ++GL  +   +LG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 181 NCVSPHVVPTQMTRDLFKLEDG--DEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFV 240
           NCVSP+VV + +  D+F ++    +E  +  ++LK G +L  ED+A+A  YL  D SK+V
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLK-GILLRAEDVADAVAYLAGDESKYV 252

Query: 241 SGHNLIVDGGFT 249
           SG NL++DGG+T
Sbjct: 253 SGLNLVIDGGYT 263

[Close]

BLAST of MC03g0447 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.3e-67
Identity = 134/257 (52.14%), Postives = 175/257 (68.09%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNL-CPSSSSFVHCD 60
           RLEGKVAL+TGGA GIGE  ARLF +HGAK+ I D+QD+LG  +S  L     + + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 61  VTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLG 120
           VT E+DV  AVD T  KYG +DIM NNAGI+GD   +I + +  +F+KV ++N+ G FLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 121 TKHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVN 180
            KHAAR M+P  +GSI+  AS  SVI GA PH YT +KH +VGL K+ A +LGR+GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 181 CVSPHVVPTQMTRDLFKLEDGDE--------FPNHYSHLKCGGMLMEEDIAEAALYLGSD 240
           CVSP+ VPT+++       +  E        F    ++LK G  LM  D+AEA LYL ++
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLK-GVDLMPNDVAEAVLYLATE 241

Query: 241 ASKFVSGHNLIVDGGFT 249
            SK+VSG NL++DGGF+
Sbjct: 242 ESKYVSGLNLVIDGGFS 257

[Close]

BLAST of MC03g0447 vs. NCBI nr
Match: XP_022142407.1 (momilactone A synthase-like [Momordica charantia])

HSP 1 Score: 498 bits (1283), Expect = 1.70e-177
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. NCBI nr
Match: XP_022927564.1 (secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 418 bits (1074), Expect = 1.04e-145
Identity = 206/248 (83.06%), Postives = 226/248 (91.13%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E D+ NAV+T +SKYGKLDIM NNAGISG LKFNILENE  DFQKVLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAA+AM+PAR+GSII+ ASGCS+IGG  P+AYTSSKHG++GLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. NCBI nr
Match: XP_022927566.1 (secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 418 bits (1074), Expect = 1.04e-145
Identity = 206/248 (83.06%), Postives = 226/248 (91.13%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E D+ NAV+T +SKYGKLDIM NNAGISG LKFNILENE  DFQKVLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAA+AM+PAR+GSII+ ASGCS+IGG  P+AYTSSKHG++GLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. NCBI nr
Match: XP_022927565.1 (secoisolariciresinol dehydrogenase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 416 bits (1069), Expect = 6.00e-145
Identity = 205/247 (83.00%), Postives = 225/247 (91.09%), Query Frame = 0


Query: 2   LEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDVT 61
           LEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDVT
Sbjct: 13  LEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDVT 72

Query: 62  RENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGTK 121
           +E D+ NAV+T +SKYGKLDIM NNAGISG LKFNILENE  DFQKVLNVNLLGAFLGTK
Sbjct: 73  KEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGTK 132

Query: 122 HAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNCV 181
           HAA+AM+PAR+GSII+ ASGCS+IGG  P+AYTSSKHG++GLMKNA VDLGRYGIRVNCV
Sbjct: 133 HAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNCV 192

Query: 182 SPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHNL 241
           SPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHNL
Sbjct: 193 SPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHNL 252

Query: 242 IVDGGFT 248
           IVDGGFT
Sbjct: 253 IVDGGFT 259

[Close]

BLAST of MC03g0447 vs. NCBI nr
Match: XP_022973162.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 414 bits (1064), Expect = 3.46e-144
Identity = 205/248 (82.66%), Postives = 223/248 (89.92%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E D+ NAV+T ++KYGKLDIM NNAGISG LKFN LENES DFQ VLNVNLLG FLGT
Sbjct: 72  TKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVLNVNLLGTFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAARAM+PAR+GSIIMTAS CSVIGG  P+ YTSSKHG++GLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. ExPASy TrEMBL
Match: A0A6J1CMN7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 8.25e-178
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV
Sbjct: 12  RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT
Sbjct: 72  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC
Sbjct: 132 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN
Sbjct: 192 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. ExPASy TrEMBL
Match: A0A6J1EID0 (secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 5.03e-146
Identity = 206/248 (83.06%), Postives = 226/248 (91.13%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E D+ NAV+T +SKYGKLDIM NNAGISG LKFNILENE  DFQKVLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAA+AM+PAR+GSII+ ASGCS+IGG  P+AYTSSKHG++GLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. ExPASy TrEMBL
Match: A0A6J1EI14 (secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 5.03e-146
Identity = 206/248 (83.06%), Postives = 226/248 (91.13%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E D+ NAV+T +SKYGKLDIM NNAGISG LKFNILENE  DFQKVLNVNLLGAFLGT
Sbjct: 72  TKEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAA+AM+PAR+GSII+ ASGCS+IGG  P+AYTSSKHG++GLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. ExPASy TrEMBL
Match: A0A6J1EPB9 (secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434358 PE=4 SV=1)

HSP 1 Score: 416 bits (1069), Expect = 2.90e-145
Identity = 205/247 (83.00%), Postives = 225/247 (91.09%), Query Frame = 0


Query: 2   LEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDVT 61
           LEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDVT
Sbjct: 13  LEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDVT 72

Query: 62  RENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGTK 121
           +E D+ NAV+T +SKYGKLDIM NNAGISG LKFNILENE  DFQKVLNVNLLGAFLGTK
Sbjct: 73  KEKDIENAVNTAISKYGKLDIMLNNAGISGTLKFNILENELSDFQKVLNVNLLGAFLGTK 132

Query: 122 HAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNCV 181
           HAA+AM+PAR+GSII+ ASGCS+IGG  P+AYTSSKHG++GLMKNA VDLGRYGIRVNCV
Sbjct: 133 HAAKAMIPARQGSIIIMASGCSIIGGIGPYAYTSSKHGLLGLMKNATVDLGRYGIRVNCV 192

Query: 182 SPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHNL 241
           SPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHNL
Sbjct: 193 SPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRPEDIAEAALYLASDASRFVSGHNL 252

Query: 242 IVDGGFT 248
           IVDGGFT
Sbjct: 253 IVDGGFT 259

[Close]

BLAST of MC03g0447 vs. ExPASy TrEMBL
Match: A0A6J1I6S5 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471670 PE=4 SV=1)

HSP 1 Score: 414 bits (1064), Expect = 1.68e-144
Identity = 205/248 (82.66%), Postives = 223/248 (89.92%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RLEGKVA+ITG ARGIGE TA+LFFKHGAKVV+ADI DD+GHSLS +LC SSSSFVHCDV
Sbjct: 12  RLEGKVAVITGAARGIGESTAKLFFKHGAKVVLADILDDVGHSLSRHLCSSSSSFVHCDV 71

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E D+ NAV+T ++KYGKLDIM NNAGISG LKFN LENES DFQ VLNVNLLG FLGT
Sbjct: 72  TKEKDIENAVNTAIAKYGKLDIMLNNAGISGTLKFNFLENESSDFQNVLNVNLLGTFLGT 131

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAARAM+PAR+GSIIMTAS CSVIGG  P+ YTSSKHG++GLMKNA VDLGRYGIRVNC
Sbjct: 132 KHAARAMIPARQGSIIMTASVCSVIGGIGPYGYTSSKHGVLGLMKNATVDLGRYGIRVNC 191

Query: 181 VSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVSGHN 240
           VSPHVVP+QMTRDLFKLEDGDEFPN YSH KCG +L  EDIAEAALYL SDAS+FVSGHN
Sbjct: 192 VSPHVVPSQMTRDLFKLEDGDEFPNVYSHFKCGDILRAEDIAEAALYLASDASRFVSGHN 251

Query: 241 LIVDGGFT 248
           LIVDGGFT
Sbjct: 252 LIVDGGFT 259

[Close]

BLAST of MC03g0447 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 2.2e-65
Identity = 138/266 (51.88%), Postives = 180/266 (67.67%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSS----FV 60
           RLEGKVA+ITGGA GIG+ T  LF +HGA VVIAD+ +  G SL+ +L    +S    F+
Sbjct: 31  RLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAFI 90

Query: 61  HCDVTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLK--FNILENESLDFQKVLNVNLL 120
            CDV+ E DV N V+ TV++YG+LDI+FNNAG+ GD K   +IL+ ++ +F  V+ VN+ 
Sbjct: 91  SCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNVR 150

Query: 121 GAFLGTKHAARAMMP-ARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGR 180
           G  LG KH ARAM+    +G II TAS   V+GG  PHAYT+SKH +VGL KNAA +LG+
Sbjct: 151 GVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELGK 210

Query: 181 YGIRVNCVSPHVVPTQMTRDLFK-----------LEDGDEFPNHYSHLKCGGMLMEEDIA 240
           YGIRVNC+SP  V T M  + ++           +E+ +EF    ++LK G  L   DIA
Sbjct: 211 YGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDIA 270

Query: 241 EAALYLGSDASKFVSGHNLIVDGGFT 249
           EAALYL SD SK+V+GHNL+VDGG T
Sbjct: 271 EAALYLASDESKYVNGHNLVVDGGVT 295

[Close]

BLAST of MC03g0447 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 224.9 bits (572), Expect = 6.9e-59
Identity = 122/260 (46.92%), Postives = 164/260 (63.08%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSS----FV 60
           RL GKVALITGGA GIGE   RLF KHGAKV I D+QDDLG  +  +L    S     F+
Sbjct: 17  RLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESKETAFFI 76

Query: 61  HCDVTRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGA 120
           H DV  E+D++NAVD  V  +G LDI+ NNAG+ G    +I      +F+   +VN+ GA
Sbjct: 77  HGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFDVNVKGA 136

Query: 121 FLGTKHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGI 180
           FL  KHAAR M+P ++GSI+   S   V+GG  PH+Y  SKH ++GL ++ A +LG++GI
Sbjct: 137 FLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAELGQHGI 196

Query: 181 RVNCVSPHVVPTQMTRDLFKLEDGDE--------FPNHYSHLKCGGMLMEEDIAEAALYL 240
           RVNCVSP+ V T++       E+  E        F    ++LK G  L  +D+A A L+L
Sbjct: 197 RVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLK-GVELTVDDVANAVLFL 256

Query: 241 GSDASKFVSGHNLIVDGGFT 249
            SD S+++SG NL++DGGFT
Sbjct: 257 ASDDSRYISGDNLMIDGGFT 275

[Close]

BLAST of MC03g0447 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.1 bits (562), Expect = 1.0e-57
Identity = 119/256 (46.48%), Postives = 164/256 (64.06%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           +LEGKVALITGGA G+G+ TA  F +HGA+VVIAD+  + G   +  L  S + FV CDV
Sbjct: 40  KLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKEL-GSEAEFVRCDV 99

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDL-KFNILENESLDFQKVLNVNLLGAFLG 120
           T E D+A AV+ TV +YGKLD+M+NNAGI G +   +I + +  +F++V+ +N+ G   G
Sbjct: 100 TVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVVSG 159

Query: 121 TKHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVN 180
            KHAA+ M+PAR G I+ T+S   V GG  PH+YT SK    G++K+AA +L  +G+R+N
Sbjct: 160 IKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVRIN 219

Query: 181 CVSPHVVPTQMTRDLFKL-------EDGDEFPNHYSHLKCGGMLMEEDIAEAALYLGSDA 240
           C+SP  V T +T    +        E   E       LK G    E D+A+AALYL S+ 
Sbjct: 220 CISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELK-GAECEEADVAKAALYLASND 279

Query: 241 SKFVSGHNLIVDGGFT 249
            K+V+GHNL+VDGG T
Sbjct: 280 GKYVTGHNLVVDGGMT 293

[Close]

BLAST of MC03g0447 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 220.3 bits (560), Expect = 1.7e-57
Identity = 118/251 (47.01%), Postives = 169/251 (67.33%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           RL+GK+ +ITGGA GIG  + RLF +HGA+VVI D+QD+LG +++ ++    +S+ HCDV
Sbjct: 5   RLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCDV 64

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T E +V NAV  TV KYGKLD++F+NAG+      +IL+    +  + + +NL G     
Sbjct: 65  TNETEVENAVKFTVEKYGKLDVLFSNAGVIEPF-VSILDLNLNELDRTIAINLRGTAAFI 124

Query: 121 KHAARAMM-PARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVN 180
           KHAARAM+    RGSI+ T S  + I G  PH YT+SKHG++GL+K+A+  LG+YGIRVN
Sbjct: 125 KHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRVN 184

Query: 181 CVSPHVVPTQMTRDLFKLEDG--DEFPNHYSHLKCGGMLMEEDIAEAALYLGSDASKFVS 240
            V+P  V T +  + FK+E    ++  +  ++LK G +L    +AEAAL+L SD S +VS
Sbjct: 185 GVAPFGVATPLVCNGFKMEPNVVEQNTSASANLK-GIVLKARHVAEAALFLASDESAYVS 244

Query: 241 GHNLIVDGGFT 249
           G NL VDGG++
Sbjct: 245 GQNLAVDGGYS 253

[Close]

BLAST of MC03g0447 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 2.9e-57
Identity = 119/254 (46.85%), Postives = 162/254 (63.78%), Query Frame = 0


Query: 1   RLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNNLCPSSSSFVHCDV 60
           +LEGKVALITGGA GIG+ TA  F  HGAKV+IADIQ  +G      L PS + F  CDV
Sbjct: 77  KLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPSCAYF-PCDV 136

Query: 61  TRENDVANAVDTTVSKYGKLDIMFNNAGISGDLKFNILENESLDFQKVLNVNLLGAFLGT 120
           T+E+D+ANAVD  VS + KLDIM+NNAGI      +I++ +   F KV+N N+ G   G 
Sbjct: 137 TKESDIANAVDFAVSLHTKLDIMYNNAGIPCKTPPSIVDLDLNVFDKVINTNVRGVMAGI 196

Query: 121 KHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNAAVDLGRYGIRVNC 180
           KHAAR M+P   GSII   S   ++GG   H Y+ SK  ++G++++ A +L ++ IRVNC
Sbjct: 197 KHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRIRVNC 256

Query: 181 VSPHVVPTQMTRD-LFKLEDGDEFPNHYSHLKCGGMLMEE-----DIAEAALYLGSDASK 240
           +SP  + T    D + ++  G +       ++  G+L  E     D+A AA+YL SD SK
Sbjct: 257 ISPFAITTSFVMDEMRQIYPGVDDSRLIQIVQSTGVLNGEVCEPTDVANAAVYLASDDSK 316

Query: 241 FVSGHNLIVDGGFT 249
           +V+GHNL+VDGGFT
Sbjct: 317 YVNGHNLVVDGGFT 329

The following BLAST results are available for this feature:

BLAST of MC03g0447 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	3.0e-75	56.40	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	3.3e-74	58.80	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q94KL8	4.7e-68	51.59	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
A3F5F0	1.4e-67	51.59	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
F1SWA0	2.3e-67	52.14	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of MC03g0447 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142407.1	1.70e-177	100.00	momilactone A synthase-like [Momordica charantia]	[more]
XP_022927564.1	1.04e-145	83.06	secoisolariciresinol dehydrogenase-like isoform X1 [Cucurbita moschata]	[more]
XP_022927566.1	1.04e-145	83.06	secoisolariciresinol dehydrogenase-like isoform X3 [Cucurbita moschata]	[more]
XP_022927565.1	6.00e-145	83.00	secoisolariciresinol dehydrogenase-like isoform X2 [Cucurbita moschata]	[more]
XP_022973162.1	3.46e-144	82.66	secoisolariciresinol dehydrogenase-like [Cucurbita maxima]	[more]

BLAST of MC03g0447 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMN7	8.25e-178	100.00	momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 ...	[more]
A0A6J1EID0	5.03e-146	83.06	secoisolariciresinol dehydrogenase-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EI14	5.03e-146	83.06	secoisolariciresinol dehydrogenase-like isoform X3 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1EPB9	2.90e-145	83.00	secoisolariciresinol dehydrogenase-like isoform X2 OS=Cucurbita moschata OX=3662...	[more]
A0A6J1I6S5	1.68e-144	82.66	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111471...	[more]

BLAST of MC03g0447 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51680.1	2.2e-65	51.88	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	6.9e-59	46.92	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	1.0e-57	46.48	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	1.7e-57	47.01	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G03140.1	2.9e-57	46.85	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 153..172 score: 35.56 coord: 127..143 score: 30.64 coord: 6..23 score: 50.34 coord: 174..191 score: 42.1 coord: 78..89 score: 58.12 coord: 211..231 score: 27.75
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 153..172 score: 30.04 coord: 78..89 score: 51.1 coord: 133..141 score: 39.58
None	No IPR available	GENE3D	3.40.50.720		coord: 1..248 e-value: 3.6E-74 score: 251.2
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 11..248 e-value: 9.2E-64 score: 215.2
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 2..247 e-value: 1.5E-16 score: 57.4
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 8..209 e-value: 7.7E-17 score: 58.4
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 1..248
None	No IPR available	PANTHER	PTHR43180:SF30	MOMILACTONE A SYNTHASE	coord: 1..248
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 3..248

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0447.1	MC03g0447.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity