MC03g0437 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g0437 vs. ExPASy Swiss-Prot
Match: Q9SEA0 (Capsanthin/capsorubin synthase, chromoplastic OS=Citrus sinensis OX=2711 GN=CCS PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 5.0e-183
Identity = 315/487 (64.68%), Postives = 382/487 (78.44%), Query Frame = 0

Query: 3   ISDTLLLPQTSPFPPTTRRSSAPK----NGLQIRNSARSVNFLDLRPESRPDPLIFDLPL 62
           I D+   P  S FP   + + + K    +  +IR S +  NFL+L PES P+ L FDLP 
Sbjct: 19  IIDSTSSPSFSLFPLGRQNACSRKADHHHHHRIRTS-KFGNFLELTPESVPEFLDFDLPW 78

Query: 63  FRPGSDSSRFDVVVVGAGPAGLRLAGEVAG-YGIKVCCVDPSPLSPWPNNYGVWIDEFRA 122
           F P SD  R+DV+++G GPAGLRLA +V+  + +KVCCVDPSPLS WPNNYGVW+DEF  
Sbjct: 79  FHP-SDRIRYDVIIIGTGPAGLRLAEQVSSRHSVKVCCVDPSPLSTWPNNYGVWVDEFED 138

Query: 123 LGLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWE 182
           +GL DC  KTWPM  V+++D K KYL RPY +VSR  LKT+L+E C + GVKFHKAKVW 
Sbjct: 139 IGLVDCLDKTWPMTCVFINDHKTKYLDRPYGRVSRNILKTKLLENCVSNGVKFHKAKVWH 198

Query: 183 MEHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFD 242
           + HQEF+SS+ CDDG +IKASLI+DASGF++ F+EY KPRN G+QIAHGILAEVE HPFD
Sbjct: 199 VNHQEFESSIVCDDGNEIKASLIVDASGFASSFVEYDKPRNHGYQIAHGILAEVESHPFD 258

Query: 243 LNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVK 302
           L+KMVLMDWRD+HL NEPYLR  N K PTFLYAMPFDSNL+FLEETSLV RP L Y  VK
Sbjct: 259 LDKMVLMDWRDSHLGNEPYLRASNLKLPTFLYAMPFDSNLVFLEETSLVSRPVLSYKEVK 318

Query: 303 RRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGM 362
            RMAARLRH+GIRV+RV+E+EKC+IPMGGPLP IPQ+V+  GG +G++HPATGYMV   M
Sbjct: 319 SRMAARLRHMGIRVKRVIEDEKCLIPMGGPLPVIPQSVMAIGGTSGLIHPATGYMVARTM 378

Query: 363 GAAAGVARRVAECLGGGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGT 422
             A  +A  +AECLG  RMIRGR + ++VW G WP++RR  REFY FGMETLL+LDL GT
Sbjct: 379 ALAPALADAIAECLGSTRMIRGRPLHQKVWNGLWPIDRRCNREFYSFGMETLLKLDLKGT 438

Query: 423 RRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVA 482
           RRFFD FFDL+ +YW GFLSS LSL+ELA LSLS+FGH S  SR+DI+TKCP+PLV+M+ 
Sbjct: 439 RRFFDAFFDLNPYYWHGFLSSRLSLAELAGLSLSLFGHASNSSRLDIVTKCPVPLVKMMG 498

Query: 483 NLALQSI 485
           NLAL++I
Sbjct: 499 NLALETI 503

BLAST of MC03g0437 vs. ExPASy Swiss-Prot
Match: K4C9E2 (Neoxanthin synthase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NSY PE=1 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 4.5e-176
Identity = 295/468 (63.03%), Postives = 366/468 (78.21%), Query Frame = 0

Query: 17  PTTRRSSAPKNGLQIRNSARSVNFLDLRPESRPDPLIFDLPLFRPGSDSSRFDVVVVGAG 76
           PTT++ S  K  L+ ++S    +FLDL P S+P+ L  ++    P S+ ++FDV+++GAG
Sbjct: 32  PTTKKKSR-KCLLRNKSSKLFCSFLDLAPTSKPESLDVNISWVDPNSNRAQFDVIIIGAG 91

Query: 77  PAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGLQDCFHKTWPMASVYLD 136
           PAGLRLA +V+ YGIKVCCVDPSPLS WPNNYGVW+DEF  LGL+DC    WPM  V+++
Sbjct: 92  PAGLRLAEQVSKYGIKVCCVDPSPLSMWPNNYGVWVDEFENLGLEDCLDHKWPMTCVHIN 151

Query: 137 DRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEHQEFQSSVHCDDGAQIK 196
           D K KYL RPY +VSRKKLK +L+  C    VKF+KAKVW++EH+EF+SS+ CDDG +I+
Sbjct: 152 DNKTKYLGRPYGRVSRKKLKLKLLNSCVENRVKFYKAKVWKVEHEEFESSIVCDDGKKIR 211

Query: 197 ASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNKMVLMDWRDTHLANEPY 256
            SL++DASGF++ FIEY +PRN G+QIAHG+L EV+ HPFDL+KMVLMDWRD+HL NEPY
Sbjct: 212 GSLVVDASGFASDFIEYDRPRNHGYQIAHGVLVEVDNHPFDLDKMVLMDWRDSHLGNEPY 271

Query: 257 LRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRMAARLRHLGIRVRRVLE 316
           LR  N K PTFLYAMPFD +L+FLEETSLV RP L Y  VKRRM ARLRHLGI+V+ V+E
Sbjct: 272 LRVNNAKEPTFLYAMPFDRDLVFLEETSLVSRPVLSYMEVKRRMVARLRHLGIKVKSVIE 331

Query: 317 EEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGMGAAAGVARRVAECLGGGRM 376
           EEKCVIPMGGPLP IPQ V+  GG +G+VHP+TGYMV   M  A  +A  + E LG  RM
Sbjct: 332 EEKCVIPMGGPLPRIPQNVMAIGGNSGIVHPSTGYMVARSMALAPVLAEAIVEGLGSTRM 391

Query: 377 IRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGTRRFFDGFFDLDAFYWEGFL 436
           IRG  +  RVW G WP++RR VRE Y FGMETLL+LDL GTRR FD FFDLD  YW+GFL
Sbjct: 392 IRGSQLYHRVWNGLWPLDRRCVRECYSFGMETLLKLDLKGTRRLFDAFFDLDPKYWQGFL 451

Query: 437 SSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANLALQSI 485
           SS LS+ EL +LSL +FGHGS M+R+DI+TKCPLPLVR++ NLA++S+
Sbjct: 452 SSRLSVKELGLLSLCLFGHGSNMTRLDIVTKCPLPLVRLIGNLAIESL 498

BLAST of MC03g0437 vs. ExPASy Swiss-Prot
Match: Q9M424 (Neoxanthin synthase, chloroplastic OS=Solanum tuberosum OX=4113 GN=NXS PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 5.0e-175
Identity = 294/468 (62.82%), Postives = 362/468 (77.35%), Query Frame = 0

Query: 17  PTTRRSSAPKNGLQIRNSARSVNFLDLRPESRPDPLIFDLPLFRPGSDSSRFDVVVVGAG 76
           PTT++ S  K   +  +S    +FLDL P S+P+    ++ L  P S  ++FDV+++GAG
Sbjct: 32  PTTKKKSR-KCHFRNESSKLFCSFLDLAPISKPESFDVNISLVDPNSGRAQFDVIIIGAG 91

Query: 77  PAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGLQDCFHKTWPMASVYLD 136
           PAGLRLA  V+ YGIKVCCVDPSPLS WPNNYGVW+DEF  LGL+DC    WPM  V+++
Sbjct: 92  PAGLRLAEHVSKYGIKVCCVDPSPLSMWPNNYGVWVDEFENLGLEDCLDHKWPMTCVHIN 151

Query: 137 DRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEHQEFQSSVHCDDGAQIK 196
           D K KYL RPY +VSRKKLK RL+  C    VKF+KAKVW++EH+EF+SS+ CDDG +I+
Sbjct: 152 DHKTKYLGRPYGRVSRKKLKLRLLNSCVENRVKFYKAKVWKVEHEEFESSIVCDDGKKIR 211

Query: 197 ASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNKMVLMDWRDTHLANEPY 256
            SL++DASGF++ FIEY KPRN G+QIAHG+L EV+ HPFDL+KMVLMDWRD+HL NEPY
Sbjct: 212 GSLVVDASGFASDFIEYDKPRNHGYQIAHGVLVEVDNHPFDLDKMVLMDWRDSHLGNEPY 271

Query: 257 LRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRMAARLRHLGIRVRRVLE 316
           LR  N K PTFLYAMPFD NL+FLEETSLV RP L Y  VKRRM ARLRHLGI+VR V+E
Sbjct: 272 LRVNNAKEPTFLYAMPFDRNLVFLEETSLVSRPVLSYMEVKRRMVARLRHLGIKVRSVIE 331

Query: 317 EEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGMGAAAGVARRVAECLGGGRM 376
           EEKCVIPMGGPLP IPQ V+  GG +G+VHP+TGYMV   M  A  +A  + + LG  RM
Sbjct: 332 EEKCVIPMGGPLPRIPQNVMAIGGNSGIVHPSTGYMVARSMALAPVLAEAIVKGLGSTRM 391

Query: 377 IRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGTRRFFDGFFDLDAFYWEGFL 436
           IRG  +  RVW G WP++RR + E Y FGMETLL+LDL GTRR FD FFDLD  YW+GFL
Sbjct: 392 IRGSQLYHRVWNGLWPLDRRCIGECYSFGMETLLKLDLKGTRRLFDAFFDLDPKYWQGFL 451

Query: 437 SSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANLALQSI 485
           SS LS+ ELA+LSL +FGHGS ++R+DI+TKCP+PLVR++ NLA++S+
Sbjct: 452 SSRLSVKELAILSLCLFGHGSNLTRLDIVTKCPVPLVRLIGNLAIESL 498

BLAST of MC03g0437 vs. ExPASy Swiss-Prot
Match: Q42435 (Capsanthin/capsorubin synthase, chromoplastic OS=Capsicum annuum OX=4072 GN=CCS PE=1 SV=2)

HSP 1 Score: 605.1 bits (1559), Expect = 6.8e-172
Identity = 288/472 (61.02%), Postives = 361/472 (76.48%), Query Frame = 0

Query: 13  SPFPPTTRRSSAPKNGLQIRNSARSVNFLDLRPESRPDPLIFDLPLFRPGSDSSRFDVVV 72
           S FP  T++  + K   + ++S    +FLDL P S+P+ L  ++       D + FDV++
Sbjct: 27  STFPNPTKQKDSRKFHYRNKSSTHFCSFLDLAPTSKPESLDVNISWVDTDLDRAEFDVII 86

Query: 73  VGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGLQDCFHKTWPMAS 132
           +G GPAGLRLA +V+ YGIKVCCVDPSPLS WPNNYGVW+DEF  LGL+DC    WP++ 
Sbjct: 87  IGTGPAGLRLAEQVSKYGIKVCCVDPSPLSMWPNNYGVWVDEFEKLGLEDCLDHKWPVSC 146

Query: 133 VYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEHQEFQSSVHCDDG 192
           V++ D K KYL RPY +VSRKKLK +L+  C    VKF+KAKV +++H+EF+SS+ CDDG
Sbjct: 147 VHISDHKTKYLDRPYGRVSRKKLKLKLLNSCVENRVKFYKAKVLKVKHEEFESSIVCDDG 206

Query: 193 AQIKASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNKMVLMDWRDTHLA 252
            +I  SLI+DASG+++ FIEY KPRN G+Q+AHGILAEV+ HPFDL+KM+LMDWRD+HL 
Sbjct: 207 RKISGSLIVDASGYASDFIEYDKPRNHGYQVAHGILAEVDNHPFDLDKMMLMDWRDSHLG 266

Query: 253 NEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRMAARLRHLGIRVR 312
           NEPYLR +N K PTFLYAMPFD NL+FLEETSLV RP L Y  VKRRM ARLRHLGI+VR
Sbjct: 267 NEPYLRVKNTKEPTFLYAMPFDRNLVFLEETSLVSRPMLSYMEVKRRMVARLRHLGIKVR 326

Query: 313 RVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGMGAAAGVARRVAECLG 372
            VLEEEKCVI MGGPLP IPQ V+  GG +G+VHP++GYMV   M  A  +A  + E LG
Sbjct: 327 SVLEEEKCVITMGGPLPRIPQNVMAIGGTSGIVHPSSGYMVARSMALAPVLAEAIVESLG 386

Query: 373 GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGTRRFFDGFFDLDAFYW 432
             RMIRG  +  RVW G WP +RRRVRE YCFGMETLL+LDL+GTRR FD FFD+D  YW
Sbjct: 387 STRMIRGSQLYHRVWNGLWPSDRRRVRECYCFGMETLLKLDLEGTRRLFDAFFDVDPKYW 446

Query: 433 EGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANLALQSI 485
            GFLSS LS+ ELA+LSL +FGH S ++R+DI+TKC +PLV+++ NLA++S+
Sbjct: 447 HGFLSSRLSVKELAVLSLYLFGHASNLARLDIVTKCTVPLVKLLGNLAIESL 498

BLAST of MC03g0437 vs. ExPASy Swiss-Prot
Match: Q43503 (Lycopene beta cyclase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LCY1 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 6.2e-141
Identity = 243/439 (55.35%), Postives = 316/439 (71.98%), Query Frame = 0

Query: 41  LDLRPESRPDPLIFDLPLFRPGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSP 100
           L+L PE++ + L F+LP++ P S     D+ VVG GPAGL +A +V+  G+ VC +DP+P
Sbjct: 58  LELVPETKKENLDFELPMYDP-SKGVVVDLAVVGGGPAGLAVAQQVSEAGLSVCSIDPNP 117

Query: 101 LSPWPNNYGVWIDEFRALGLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLM 160
              WPNNYGVW+DEF A+ L DC   TW  A+VY+DD   K LHRPY +V+RK+LK+++M
Sbjct: 118 KLIWPNNYGVWVDEFEAMDLLDCLDATWSGAAVYIDDNTAKDLHRPYGRVNRKQLKSKMM 177

Query: 161 EECAAKGVKFHKAKVWEMEHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSKPRNCG 220
           ++C   GVKFH+AKV ++ H+E +S + C+DG  I+A++++DA+GFS   ++Y KP N G
Sbjct: 178 QKCIMNGVKFHQAKVIKVIHEESKSMLICNDGITIQATVVLDATGFSRSLVQYDKPYNPG 237

Query: 221 FQIAHGILAEVEEHPFDLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFL 280
           +Q+A+GILAEVEEHPFD+NKMV MDWRD+HL N   L++ N + PTFLYAMPF SN IFL
Sbjct: 238 YQVAYGILAEVEEHPFDVNKMVFMDWRDSHLKNNTDLKERNSRIPTFLYAMPFSSNRIFL 297

Query: 281 EETSLVGRPALPYAAVKRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGG 340
           EETSLV RP L    ++ RM ARL HLGI+V+ + E+E C+IPMGGPLP +PQ VVG GG
Sbjct: 298 EETSLVARPGLRIDDIQERMVARLNHLGIKVKSIEEDEHCLIPMGGPLPVLPQRVVGIGG 357

Query: 341 AAGMVHPATGYMVVAGMGAAAGVARRVAECLGGGRMIRGRGMRERVWGGAWPVERRRVRE 400
            AGMVHP+TGYMV   + AA  VA  + + LG  R   G  +   VW   WP+ERRR RE
Sbjct: 358 TAGMVHPSTGYMVARTLAAAPVVANAIIQYLGSERSHSGNELSTAVWKDLWPIERRRQRE 417

Query: 401 FYCFGMETLLRLDLDGTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMS 460
           F+CFGM+ LL+LDL  TRRFFD FFDL+  YW GFLSS L L EL +  LS+F H S  S
Sbjct: 418 FFCFGMDILLKLDLPATRRFFDAFFDLEPRYWHGFLSSRLFLPELIVFGLSLFSHASNTS 477

Query: 461 RVDILTKCPLPLVRMVANL 480
           R +I+TK  +PLV M+ NL
Sbjct: 478 RFEIMTKGTVPLVNMINNL 495

BLAST of MC03g0437 vs. NCBI nr
Match: XP_022973132.1 (capsanthin/capsorubin synthase, chromoplastic-like [Cucurbita maxima])

HSP 1 Score: 750 bits (1936), Expect = 2.14e-269
Identity = 372/489 (76.07%), Postives = 420/489 (85.89%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARS-VNFLDLRPESRPDPLIFDLPLF 60
           MAIS  LL P + P  P++   S  +   Q   + R   NFLDL+PES+P PL FDLPLF
Sbjct: 1   MAISHALLSPFSLPHIPSSPIHSPFRTHFQPHYTLRKFANFLDLKPESKPQPLKFDLPLF 60

Query: 61  RPGSDSSR-FDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
            P SD  R FD VVVGAGPAGLRLA +++ YGIKVCCVDPSPLSPWPNNYGVW+DEF+AL
Sbjct: 61  HPYSDRPRRFDAVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQAL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           G QDCFHKTWPMASVYL+D KVK+L RPYAQVSRKKLK +LMEECA KGVKFHKAKVWE+
Sbjct: 121 GFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSK--PRNCGFQIAHGILAEVEEHPF 240
           +HQEF+SSV CDDG +IKA+L+IDASGF+++F EYSK  PRNCGFQIAHGILAEVE+HPF
Sbjct: 181 KHQEFESSVACDDGTEIKANLVIDASGFASRFTEYSKSKPRNCGFQIAHGILAEVEQHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFD NLIFLEETSLVGRPALPYA V
Sbjct: 241 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDPNLIFLEETSLVGRPALPYAVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           KRRMAARLRHLGI+V+R+LEEEKCVIPMGG LP++PQAVV AGG AGMVHPA+GY VV G
Sbjct: 301 KRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGGVAGMVHPASGYSVVLG 360

Query: 361 MGAAAGVARRVAECLG-GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 420
           M AA GVARR AE LG GGRMIRGR ++ RVW G WP ERRRVREFYCFGMETL+RLDL+
Sbjct: 361 MAAAPGVARRAAEGLGSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLVRLDLE 420

Query: 421 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 480
           G RRFFD FFDL+ FYWEGFLSS LS+ EL ML L++FGH S MS++DILTKCPLPL++M
Sbjct: 421 GLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLALFGHASPMSKLDILTKCPLPLLKM 480

Query: 481 VANLALQSI 484
           +ANLALQ++
Sbjct: 481 MANLALQAL 489

BLAST of MC03g0437 vs. NCBI nr
Match: XP_023520686.1 (capsanthin/capsorubin synthase, chromoplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 748 bits (1932), Expect = 8.71e-269
Identity = 372/489 (76.07%), Postives = 419/489 (85.69%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSV-NFLDLRPESRPDPLIFDLPLF 60
           MAIS  LL P + P  P++   S  +   Q   + R   NFLDL+PES+P PL FDLPLF
Sbjct: 1   MAISQALLSPFSLPHIPSSPIHSPFRTHFQPHCTLRKFGNFLDLKPESKPQPLKFDLPLF 60

Query: 61  RPGSDSSR-FDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
            P SD  R FDVVVVGAGPAGLRLA +++ YGIKVCCVDPSPLSPWPNNYGVW+DEF+AL
Sbjct: 61  HPYSDRPRRFDVVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQAL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           G QDCFHKTWPMASV+L+D  VK+L RPYAQVSRKKLK +LMEECA KGVKFHKAKVWE+
Sbjct: 121 GFQDCFHKTWPMASVHLNDENVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYS--KPRNCGFQIAHGILAEVEEHPF 240
           +HQEF+SSV CDDG +IKA+L+IDASGF+++F EYS  KPRNCGFQIAHGILAEVE+HPF
Sbjct: 181 KHQEFESSVACDDGTEIKANLVIDASGFASRFTEYSNSKPRNCGFQIAHGILAEVEQHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFDSNLIFLEETSLVGRPALPYA V
Sbjct: 241 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFLEETSLVGRPALPYAVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           KRRMAARLRHLGI+V+R+LEEEKCVIPMGG LP++PQAVV AGG AGMVHPA+GY VV G
Sbjct: 301 KRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGGVAGMVHPASGYSVVLG 360

Query: 361 MGAAAGVARRVAECLG-GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 420
           M AA GVARR AE LG GGRMIRGR ++ RVW G WP ERRR REFYCFGMETL+RLDL+
Sbjct: 361 MAAAPGVARRAAEGLGSGGRMIRGRALQGRVWEGVWPAERRRAREFYCFGMETLVRLDLE 420

Query: 421 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 480
           G RRFFD FFDL+ FYWEGFLSS LS+ EL ML LS+FGH S MS++DILTKCPLPL +M
Sbjct: 421 GLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLSLFGHASPMSKLDILTKCPLPLAKM 480

Query: 481 VANLALQSI 484
           +ANLALQ++
Sbjct: 481 MANLALQAL 489

BLAST of MC03g0437 vs. NCBI nr
Match: XP_022927350.1 (capsanthin/capsorubin synthase, chromoplastic-like [Cucurbita moschata])

HSP 1 Score: 748 bits (1932), Expect = 8.71e-269
Identity = 373/489 (76.28%), Postives = 419/489 (85.69%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSV-NFLDLRPESRPDPLIFDLPLF 60
           MAIS  LL P + P  P++   S  +   Q   + R   NFLDL+PES+P PL FDLPLF
Sbjct: 1   MAISHALLSPFSLPDIPSSPIHSPFRTHFQPHCTLRRFGNFLDLKPESKPQPLKFDLPLF 60

Query: 61  RPGSDSSR-FDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
            P SD  R FDVVVVGAGPAGLRLA +++ YGIKVCCVDPSPLSPWPNNYGVW+DEF+AL
Sbjct: 61  HPYSDRPRRFDVVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQAL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           G QDCFHKTWPMASVYL+D KVK+L RPYAQVSRKKLK +LMEECA KGVKFHKAKVWE+
Sbjct: 121 GFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYS--KPRNCGFQIAHGILAEVEEHPF 240
           +HQEF+SSV CDDG +IKA+L+ID SGF+++F EYS  KPRNCGFQIAHGILAEVE+HPF
Sbjct: 181 KHQEFESSVACDDGTEIKANLVIDTSGFASRFTEYSNSKPRNCGFQIAHGILAEVEQHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFDSNLIFLEETSLVGRPALPYA V
Sbjct: 241 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFLEETSLVGRPALPYAVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           KRRMAARLRHLGI+V+R+LEEEKCVIPMGG LP++PQAVV AGG AGMVHPA+GY VV G
Sbjct: 301 KRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGGVAGMVHPASGYSVVLG 360

Query: 361 MGAAAGVARRVAECLG-GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 420
           M AA GVARR AE LG GGRMIRGR ++ RVW G WP ERRRVREFYCFGMETL+RLDL+
Sbjct: 361 MAAAPGVARRAAEGLGSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLVRLDLE 420

Query: 421 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 480
           G RRFFD FFDL+ FYWEGFLSS LS+ EL ML L +FGH S MS++DILTKCPLPL +M
Sbjct: 421 GLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLLLFGHASPMSKLDILTKCPLPLAKM 480

Query: 481 VANLALQSI 484
           +ANLALQ++
Sbjct: 481 MANLALQAL 489

BLAST of MC03g0437 vs. NCBI nr
Match: KAG7019363.1 (Capsanthin/capsorubin synthase, chromoplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 747 bits (1928), Expect = 3.54e-268
Identity = 372/489 (76.07%), Postives = 418/489 (85.48%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSV-NFLDLRPESRPDPLIFDLPLF 60
           MAIS  LL P + P  P++   S  +   Q   + R   NFLDL+PES+P PL FDLPLF
Sbjct: 1   MAISHALLSPFSLPDIPSSPIHSPFRTHFQPHCTLRRFGNFLDLKPESKPQPLKFDLPLF 60

Query: 61  RPGSDSSR-FDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
            P SD  R FDVVVVGAGPAGLRLA +++ YGIKVCCVDPSPLSPWPNNYGVW+DEF+AL
Sbjct: 61  HPYSDRPRRFDVVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQAL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           G QDCFHKTWPMASVYL+D KVK+L RPYAQVSRKKLK +LMEECA KGVKFHKAKVWE+
Sbjct: 121 GFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYS--KPRNCGFQIAHGILAEVEEHPF 240
           +HQEF+SSV CDDG +IKA+L+ID SGF+++F EYS  KPRNCGFQIAHGILAEVE+HPF
Sbjct: 181 KHQEFESSVACDDGTEIKANLVIDTSGFASRFTEYSNSKPRNCGFQIAHGILAEVEQHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFDSNLIFLEETSLVGRPALPYA V
Sbjct: 241 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFLEETSLVGRPALPYAVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           KRRMAARLRHLGI+V+R+LEEEKCVIPMGG LP++PQAVV AGG AGMVHPA+GY VV G
Sbjct: 301 KRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGGVAGMVHPASGYSVVLG 360

Query: 361 MGAAAGVARRVAECLG-GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 420
           M AA GVARR  E LG GGRMIRGR ++ RVW G WP ERRRVREFYCFGMETL+RLDL+
Sbjct: 361 MAAAPGVARRAVEGLGSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLVRLDLE 420

Query: 421 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 480
           G RRFFD FFDL+ FYWEGFLSS LS+ EL ML L +FGH S MS++DILTKCPLPL +M
Sbjct: 421 GLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLLLFGHASPMSKLDILTKCPLPLAKM 480

Query: 481 VANLALQSI 484
           +ANLALQ++
Sbjct: 481 MANLALQAL 489

BLAST of MC03g0437 vs. NCBI nr
Match: XP_038903700.1 (capsanthin/capsorubin synthase, chromoplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 748 bits (1930), Expect = 5.93e-268
Identity = 370/488 (75.82%), Postives = 416/488 (85.25%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNS-ARSVNFLDLRPESRPDPLIFDLPLF 60
           MA S  LL P + P  P++   S   N  QI  S  +  NFLDL+PES+P+PL  DLPLF
Sbjct: 34  MANSQALLSPFSLPQLPSSPIHSPSTNHFQIHCSFGKFGNFLDLKPESKPEPLKLDLPLF 93

Query: 61  RPGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALG 120
            P SD SRFDVVV+GAGPAGLRLA ++ G+GIKVCC+DPSPLSPWPNNYGVW+DEF ALG
Sbjct: 94  HPYSDRSRFDVVVIGAGPAGLRLAEQLGGFGIKVCCMDPSPLSPWPNNYGVWVDEFEALG 153

Query: 121 LQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEME 180
            QDCFHKTWPMA+VYL DRKVKYL RPYAQVSRKKLK +LMEEC  KGVKFHKAKVWE++
Sbjct: 154 FQDCFHKTWPMATVYLSDRKVKYLDRPYAQVSRKKLKMKLMEECVRKGVKFHKAKVWEIK 213

Query: 181 HQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSK--PRNCGFQIAHGILAEVEEHPFD 240
           HQEF+SSV C+DG +IKA+L+IDASGF++KF EYSK  PRNCGFQIAHGILAEVE HPFD
Sbjct: 214 HQEFESSVSCNDGTEIKANLVIDASGFASKFTEYSKSKPRNCGFQIAHGILAEVERHPFD 273

Query: 241 LNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVK 300
           LNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFDSNLIF+EETSLVGRPALPY  VK
Sbjct: 274 LNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFMEETSLVGRPALPYTEVK 333

Query: 301 RRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGM 360
           RRMAARLRHLGI+V+R+LEEEKCVIPMGGPLPE+PQA V AGG AGMV+PATGY VV GM
Sbjct: 334 RRMAARLRHLGIKVKRILEEEKCVIPMGGPLPEMPQAAVAAGGVAGMVNPATGYAVVGGM 393

Query: 361 GAAAGVARRVAECLGGG-RMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDG 420
            AA  VAR  AE LGGG RMIRGR ++ RVW G WP+E+R VREFYCFGMETL+RLDLDG
Sbjct: 394 AAAPRVARMAAEGLGGGGRMIRGRALQGRVWEGVWPMEKRSVREFYCFGMETLVRLDLDG 453

Query: 421 TRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMV 480
            RRFFD FFDL+ FYWEGFLSS LSL EL MLSLS++GH S MS+VDILTKCPLPL +M+
Sbjct: 454 MRRFFDAFFDLEPFYWEGFLSSRLSLWELVMLSLSLYGHASLMSKVDILTKCPLPLFKMM 513

Query: 481 ANLALQSI 484
           AN+ LQ++
Sbjct: 514 ANITLQAL 521

BLAST of MC03g0437 vs. ExPASy TrEMBL
Match: A0A6J1I6P6 (capsanthin/capsorubin synthase, chromoplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111471649 PE=3 SV=1)

HSP 1 Score: 750 bits (1936), Expect = 1.04e-269
Identity = 372/489 (76.07%), Postives = 420/489 (85.89%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARS-VNFLDLRPESRPDPLIFDLPLF 60
           MAIS  LL P + P  P++   S  +   Q   + R   NFLDL+PES+P PL FDLPLF
Sbjct: 1   MAISHALLSPFSLPHIPSSPIHSPFRTHFQPHYTLRKFANFLDLKPESKPQPLKFDLPLF 60

Query: 61  RPGSDSSR-FDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
            P SD  R FD VVVGAGPAGLRLA +++ YGIKVCCVDPSPLSPWPNNYGVW+DEF+AL
Sbjct: 61  HPYSDRPRRFDAVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQAL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           G QDCFHKTWPMASVYL+D KVK+L RPYAQVSRKKLK +LMEECA KGVKFHKAKVWE+
Sbjct: 121 GFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSK--PRNCGFQIAHGILAEVEEHPF 240
           +HQEF+SSV CDDG +IKA+L+IDASGF+++F EYSK  PRNCGFQIAHGILAEVE+HPF
Sbjct: 181 KHQEFESSVACDDGTEIKANLVIDASGFASRFTEYSKSKPRNCGFQIAHGILAEVEQHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFD NLIFLEETSLVGRPALPYA V
Sbjct: 241 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDPNLIFLEETSLVGRPALPYAVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           KRRMAARLRHLGI+V+R+LEEEKCVIPMGG LP++PQAVV AGG AGMVHPA+GY VV G
Sbjct: 301 KRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGGVAGMVHPASGYSVVLG 360

Query: 361 MGAAAGVARRVAECLG-GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 420
           M AA GVARR AE LG GGRMIRGR ++ RVW G WP ERRRVREFYCFGMETL+RLDL+
Sbjct: 361 MAAAPGVARRAAEGLGSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLVRLDLE 420

Query: 421 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 480
           G RRFFD FFDL+ FYWEGFLSS LS+ EL ML L++FGH S MS++DILTKCPLPL++M
Sbjct: 421 GLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLALFGHASPMSKLDILTKCPLPLLKM 480

Query: 481 VANLALQSI 484
           +ANLALQ++
Sbjct: 481 MANLALQAL 489

BLAST of MC03g0437 vs. ExPASy TrEMBL
Match: A0A6J1ENN2 (capsanthin/capsorubin synthase, chromoplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434203 PE=3 SV=1)

HSP 1 Score: 748 bits (1932), Expect = 4.21e-269
Identity = 373/489 (76.28%), Postives = 419/489 (85.69%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSV-NFLDLRPESRPDPLIFDLPLF 60
           MAIS  LL P + P  P++   S  +   Q   + R   NFLDL+PES+P PL FDLPLF
Sbjct: 1   MAISHALLSPFSLPDIPSSPIHSPFRTHFQPHCTLRRFGNFLDLKPESKPQPLKFDLPLF 60

Query: 61  RPGSDSSR-FDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
            P SD  R FDVVVVGAGPAGLRLA +++ YGIKVCCVDPSPLSPWPNNYGVW+DEF+AL
Sbjct: 61  HPYSDRPRRFDVVVVGAGPAGLRLAEQLSAYGIKVCCVDPSPLSPWPNNYGVWVDEFQAL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           G QDCFHKTWPMASVYL+D KVK+L RPYAQVSRKKLK +LMEECA KGVKFHKAKVWE+
Sbjct: 121 GFQDCFHKTWPMASVYLNDEKVKFLDRPYAQVSRKKLKMKLMEECATKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYS--KPRNCGFQIAHGILAEVEEHPF 240
           +HQEF+SSV CDDG +IKA+L+ID SGF+++F EYS  KPRNCGFQIAHGILAEVE+HPF
Sbjct: 181 KHQEFESSVACDDGTEIKANLVIDTSGFASRFTEYSNSKPRNCGFQIAHGILAEVEQHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK+PTFLYAMPFDSNLIFLEETSLVGRPALPYA V
Sbjct: 241 DLNKMVLMDWRDTHLGNEPYLRQDNKKSPTFLYAMPFDSNLIFLEETSLVGRPALPYAVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           KRRMAARLRHLGI+V+R+LEEEKCVIPMGG LP++PQAVV AGG AGMVHPA+GY VV G
Sbjct: 301 KRRMAARLRHLGIKVKRILEEEKCVIPMGGSLPKMPQAVVAAGGVAGMVHPASGYSVVLG 360

Query: 361 MGAAAGVARRVAECLG-GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 420
           M AA GVARR AE LG GGRMIRGR ++ RVW G WP ERRRVREFYCFGMETL+RLDL+
Sbjct: 361 MAAAPGVARRAAEGLGSGGRMIRGRALQGRVWEGVWPAERRRVREFYCFGMETLVRLDLE 420

Query: 421 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 480
           G RRFFD FFDL+ FYWEGFLSS LS+ EL ML L +FGH S MS++DILTKCPLPL +M
Sbjct: 421 GLRRFFDAFFDLEGFYWEGFLSSRLSIRELGMLGLLLFGHASPMSKLDILTKCPLPLAKM 480

Query: 481 VANLALQSI 484
           +ANLALQ++
Sbjct: 481 MANLALQAL 489

BLAST of MC03g0437 vs. ExPASy TrEMBL
Match: A0A6J1CNU7 (capsanthin/capsorubin synthase, chromoplastic-like OS=Momordica charantia OX=3673 GN=LOC111012727 PE=3 SV=1)

HSP 1 Score: 729 bits (1883), Expect = 2.23e-263
Identity = 382/485 (78.76%), Postives = 382/485 (78.76%), Query Frame = 0

Query: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSVNFLDLRPESRPDPLIFDLPLFR 60
           MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSVNFLDLRPESRPDPLIFDLPLFR
Sbjct: 1   MAISDTLLLPQTSPFPPTTRRSSAPKNGLQIRNSARSVNFLDLRPESRPDPLIFDLPLFR 60

Query: 61  PGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGL 120
           PGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGL
Sbjct: 61  PGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGL 120

Query: 121 QDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEH 180
           QDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEH
Sbjct: 121 QDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEH 180

Query: 181 QEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNK 240
           QEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNK
Sbjct: 181 QEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNK 240

Query: 241 MVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRM 300
           MVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRM
Sbjct: 241 MVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRM 300

Query: 301 AARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGMGAA 360
           AARLRHLGIR                                                  
Sbjct: 301 AARLRHLGIR-------------------------------------------------- 360

Query: 361 AGVARRVAECLGGGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGTRRF 420
                                                                LDGTRRF
Sbjct: 361 -----------------------------------------------------LDGTRRF 382

Query: 421 FDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANLA 480
           FDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANLA
Sbjct: 421 FDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANLA 382

Query: 481 LQSIR 485
           LQSIR
Sbjct: 481 LQSIR 382

BLAST of MC03g0437 vs. ExPASy TrEMBL
Match: A0A1S3BZM2 (capsanthin/capsorubin synthase, chromoplastic-like OS=Cucumis melo OX=3656 GN=LOC103494777 PE=3 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 2.73e-249
Identity = 352/490 (71.84%), Postives = 402/490 (82.04%), Query Frame = 0

Query: 1   MAISDTLLLPQTS-PFPPTTRRSSAPKNGLQIRNS-ARSVNFLDLRPESRPDPLIFDLPL 60
           MA S  LL P  S P  P+    S  KN LQI ++ A+  NFLDL+PES+P PL  DLPL
Sbjct: 1   MANSRFLLSPFFSLPQLPSNPIQSPSKNHLQIHSTFAKFDNFLDLKPESKPKPLKLDLPL 60

Query: 61  FRPGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
           F P  D SRFDVVVVGAGP GLR+A ++ G+GIKVCCVDPSPLSPW NNYGVW+DEF  L
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           GL+DC HKTWPMAS+YL D KVKYL RPYAQVSRKKLK +LMEEC  KGVKFHKAKVWE+
Sbjct: 121 GLEDCLHKTWPMASLYLSDEKVKYLDRPYAQVSRKKLKMKLMEECIRKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYS--KPRNCGFQIAHGILAEVEEHPF 240
            HQ+F+SSV C+DG +IK++L+IDASGF++KF +YS   PRNC FQIAHGILAEV+ HPF
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSDSNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ NK+ PTFLYAMPFDSNLIFLEETSLVGRPALPY  V
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQHNKEFPTFLYAMPFDSNLIFLEETSLVGRPALPYTVV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           K+RMAARLRHLGI+V+R+LEEEKCVIPMGG LPE+PQAVVG GG AGMV+PA+GY VV G
Sbjct: 301 KKRMAARLRHLGIKVKRILEEEKCVIPMGGALPEMPQAVVGVGGVAGMVNPASGYAVVKG 360

Query: 361 MGAAAGVARRVAECLGGG--RMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDL 420
           M AA  +AR   E +GGG  RMIRGR +  RVW G WP+E R VREFYCFGME L+RLDL
Sbjct: 361 MAAAGRIARVAGEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILMRLDL 420

Query: 421 DGTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVR 480
           DG R FF+ FFDL+ FYWEGFLSS LSL ELAMLSLS++ H S  S++DILTK PLPL++
Sbjct: 421 DGIRGFFNAFFDLEPFYWEGFLSSRLSLLELAMLSLSLYQHASFRSKLDILTKSPLPLLQ 480

Query: 481 MVANLALQSI 484
           M+ANL LQ++
Sbjct: 481 MMANLTLQAL 490

BLAST of MC03g0437 vs. ExPASy TrEMBL
Match: A0A0A0KY78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001730 PE=3 SV=1)

HSP 1 Score: 693 bits (1788), Expect = 3.68e-247
Identity = 347/490 (70.82%), Postives = 402/490 (82.04%), Query Frame = 0

Query: 1   MAISDTLLLPQTS-PFPPTTRRSSAPKNGLQIRNS-ARSVNFLDLRPESRPDPLIFDLPL 60
           MA S  LL P +  P  P+    S  KN  QI ++ A+  NFLDL+PES+P PL  DLPL
Sbjct: 1   MANSQILLSPFSCLPRLPSYPIQSPSKNRFQIHSTFAKFDNFLDLKPESKPQPLKLDLPL 60

Query: 61  FRPGSDSSRFDVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRAL 120
           F P  D SRFDVVVVGAGP GLR+A ++ G+GIKVCCVDPSPLSPW NNYGVW+DEF  L
Sbjct: 61  FHPYYDRSRFDVVVVGAGPIGLRVAEQLGGFGIKVCCVDPSPLSPWSNNYGVWVDEFEDL 120

Query: 121 GLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM 180
           GL+DC HKTWPMASVYL D KVKYL RPYAQVSRKKLK +LMEEC +KGVKFHKAKVWE+
Sbjct: 121 GLEDCLHKTWPMASVYLSDEKVKYLDRPYAQVSRKKLKMKLMEECISKGVKFHKAKVWEI 180

Query: 181 EHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSK--PRNCGFQIAHGILAEVEEHPF 240
            HQ+F+SSV C+DG +IK++L+IDASGF++KF +YS+  PRNC FQIAHGILAEV+ HPF
Sbjct: 181 NHQQFESSVSCNDGTEIKSNLVIDASGFTSKFTKYSESNPRNCAFQIAHGILAEVDHHPF 240

Query: 241 DLNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAV 300
           DLNKMVLMDWRDTHL NEPYLRQ+NKK PTFLYAMPFDSNLIFLEETSL GRPA+PY AV
Sbjct: 241 DLNKMVLMDWRDTHLNNEPYLRQDNKKFPTFLYAMPFDSNLIFLEETSLAGRPAMPYTAV 300

Query: 301 KRRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAG 360
           K+RMAARLRHLGI+V+R++EEEKCVIPMGG LP++PQAVVG GG AGMV+PA+GY VV G
Sbjct: 301 KKRMAARLRHLGIKVKRIIEEEKCVIPMGGALPKMPQAVVGVGGVAGMVNPASGYAVVKG 360

Query: 361 MGAAAGVARRVAECLGGG--RMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDL 420
           MGAA  +AR  AE +GGG  RMIRGR +  RVW G WP+E R VREFYCFGME L+RLDL
Sbjct: 361 MGAAGRIARVAAEGVGGGGGRMIRGRALEGRVWEGVWPMEERNVREFYCFGMEILVRLDL 420

Query: 421 DGTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVR 480
           DG R FF+ FFDL+  YWEG LSS LSL EL MLSLS++ H S  S++DILTK PLPL++
Sbjct: 421 DGIRGFFNAFFDLEPSYWEGLLSSRLSLLELGMLSLSLYQHASFRSKLDILTKSPLPLLQ 480

Query: 481 MVANLALQSI 484
           M+ANL L ++
Sbjct: 481 MMANLTLHAL 490

BLAST of MC03g0437 vs. TAIR 10
Match: AT3G10230.1 (lycopene cyclase )

HSP 1 Score: 498.4 bits (1282), Expect = 6.4e-141
Identity = 248/463 (53.56%), Postives = 325/463 (70.19%), Query Frame = 0

Query: 21  RSSAPKNGLQIRNS--ARSVNFLDLRPESRPDPLIFDLPLFRPGSDSSRFDVVVVGAGPA 80
           R    K  ++I +S  + S   LDL PE++ + L F+LPL+   S S   D+ +VG GPA
Sbjct: 35  RLGVKKRAIKIVSSVVSGSAALLDLVPETKKENLDFELPLY-DTSKSQVVDLAIVGGGPA 94

Query: 81  GLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGLQDCFHKTWPMASVYLDDR 140
           GL +A +V+  G+ VC +DPSP   WPNNYGVW+DEF A+ L DC   TW  A VY+D+ 
Sbjct: 95  GLAVAQQVSEAGLSVCSIDPSPKLIWPNNYGVWVDEFEAMDLLDCLDTTWSGAVVYVDEG 154

Query: 141 KVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEMEHQEFQSSVHCDDGAQIKAS 200
             K L RPY +V+RK+LK++++++C   GVKFH++KV  + H+E  S+V C DG +I+AS
Sbjct: 155 VKKDLSRPYGRVNRKQLKSKMLQKCITNGVKFHQSKVTNVVHEEANSTVVCSDGVKIQAS 214

Query: 201 LIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFDLNKMVLMDWRDTHLANEPYLR 260
           +++DA+GFS   ++Y KP N G+Q+A+GI+AEV+ HPFD++KMV MDWRD HL + P L+
Sbjct: 215 VVLDATGFSRCLVQYDKPYNPGYQVAYGIVAEVDGHPFDVDKMVFMDWRDKHLDSYPELK 274

Query: 261 QENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRMAARLRHLGIRVRRVLEEE 320
           + N K PTFLYAMPF SN IFLEETSLV RP L    ++ RMAARL+HLGI V+R+ E+E
Sbjct: 275 ERNSKIPTFLYAMPFSSNRIFLEETSLVARPGLRMEDIQERMAARLKHLGINVKRIEEDE 334

Query: 321 KCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGMGAAAGVARRVAECLG--GGRM 380
           +CVIPMGGPLP +PQ VVG GG AGMVHP+TGYMV   + AA  VA  +   LG      
Sbjct: 335 RCVIPMGGPLPVLPQRVVGIGGTAGMVHPSTGYMVARTLAAAPIVANAIVRYLGSPSSNS 394

Query: 381 IRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGTRRFFDGFFDLDAFYWEGFL 440
           +RG  +   VW   WP+ERRR REF+CFGM+ LL+LDLD TRRFFD FFDL   YW GFL
Sbjct: 395 LRGDQLSAEVWRDLWPIERRRQREFFCFGMDILLKLDLDATRRFFDAFFDLQPHYWHGFL 454

Query: 441 SSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRMVANL 480
           SS L L EL +  LS+F H S  SR++I+TK  +PL +M+ NL
Sbjct: 455 SSRLFLPELLVFGLSLFSHASNTSRLEIMTKGTVPLAKMINNL 496

BLAST of MC03g0437 vs. TAIR 10
Match: AT3G10230.2 (lycopene cyclase )

HSP 1 Score: 412.1 bits (1058), Expect = 6.0e-115
Identity = 202/364 (55.49%), Postives = 262/364 (71.98%), Query Frame = 0

Query: 118 LGLQDCFHKTWPMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWE 177
           + L DC   TW  A VY+D+   K L RPY +V+RK+LK++++++C   GVKFH++KV  
Sbjct: 1   MDLLDCLDTTWSGAVVYVDEGVKKDLSRPYGRVNRKQLKSKMLQKCITNGVKFHQSKVTN 60

Query: 178 MEHQEFQSSVHCDDGAQIKASLIIDASGFSTKFIEYSKPRNCGFQIAHGILAEVEEHPFD 237
           + H+E  S+V C DG +I+AS+++DA+GFS   ++Y KP N G+Q+A+GI+AEV+ HPFD
Sbjct: 61  VVHEEANSTVVCSDGVKIQASVVLDATGFSRCLVQYDKPYNPGYQVAYGIVAEVDGHPFD 120

Query: 238 LNKMVLMDWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVK 297
           ++KMV MDWRD HL + P L++ N K PTFLYAMPF SN IFLEETSLV RP L    ++
Sbjct: 121 VDKMVFMDWRDKHLDSYPELKERNSKIPTFLYAMPFSSNRIFLEETSLVARPGLRMEDIQ 180

Query: 298 RRMAARLRHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGM 357
            RMAARL+HLGI V+R+ E+E+CVIPMGGPLP +PQ VVG GG AGMVHP+TGYMV   +
Sbjct: 181 ERMAARLKHLGINVKRIEEDERCVIPMGGPLPVLPQRVVGIGGTAGMVHPSTGYMVARTL 240

Query: 358 GAAAGVARRVAECLG--GGRMIRGRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLD 417
            AA  VA  +   LG      +RG  +   VW   WP+ERRR REF+CFGM+ LL+LDLD
Sbjct: 241 AAAPIVANAIVRYLGSPSSNSLRGDQLSAEVWRDLWPIERRRQREFFCFGMDILLKLDLD 300

Query: 418 GTRRFFDGFFDLDAFYWEGFLSSSLSLSELAMLSLSMFGHGSTMSRVDILTKCPLPLVRM 477
            TRRFFD FFDL   YW GFLSS L L EL +  LS+F H S  SR++I+TK  +PL +M
Sbjct: 301 ATRRFFDAFFDLQPHYWHGFLSSRLFLPELLVFGLSLFSHASNTSRLEIMTKGTVPLAKM 360

Query: 478 VANL 480
           + NL
Sbjct: 361 INNL 364

BLAST of MC03g0437 vs. TAIR 10
Match: AT5G57030.1 (Lycopene beta/epsilon cyclase protein )

HSP 1 Score: 261.5 bits (667), Expect = 1.3e-69
Identity = 161/390 (41.28%), Postives = 215/390 (55.13%), Query Frame = 0

Query: 69  DVVVVGAGPAGLRLAGEVAGYGIKVCCVDPSPLSPWPNNYGVWIDEFRALGLQDCFHKTW 128
           D+VV+G GPAGL LA E A  G+KV  + P    P+ NNYGVW DEF  LGLQ C    W
Sbjct: 110 DLVVIGCGPAGLALAAESAKLGLKVGLIGPD--LPFTNNYGVWEDEFNDLGLQKCIEHVW 169

Query: 129 PMASVYLDDRKVKYLHRPYAQVSRKKLKTRLMEECAAKGVKFHKAKVWEM-EHQEFQSSV 188
               VYLDD K   + R Y +VSR+ L   L+  C   GV +  +KV  + E  +    V
Sbjct: 170 RETIVYLDDDKPITIGRAYGRVSRRLLHEELLRRCVESGVSYLSSKVDSITEASDGLRLV 229

Query: 189 HCDDGAQIKASLIIDASG-FSTKFIEY--SKPRNCGFQIAHGILAEVEEHPFDLNKMVLM 248
            CDD   I   L   ASG  S K ++Y    PR C  Q A+G+  EVE  P+D ++MV M
Sbjct: 230 ACDDNNVIPCRLATVASGAASGKLLQYEVGGPRVC-VQTAYGVEVEVENSPYDPDQMVFM 289

Query: 249 DWRDTHLANEPYLRQENKKAPTFLYAMPFDSNLIFLEETSLVGRPALPYAAVKRRMAARL 308
           D+RD    NE  +R    + PTFLYAMP   + +F EET L  +  +P+  +K ++  RL
Sbjct: 290 DYRD--YTNEK-VRSLEAEYPTFLYAMPMTKSRLFFEETCLASKDVMPFDLLKTKLMLRL 349

Query: 309 RHLGIRVRRVLEEEKCVIPMGGPLPEIPQAVVGAGGAAGMVHPATGYMVVAGMGAAAGVA 368
             LGIR+ +  EEE   IP+GG LP   Q  +  G AA MVHPATGY VV  +  A   A
Sbjct: 350 DTLGIRILKTYEEEWSYIPVGGSLPNTEQKNLAFGAAASMVHPATGYSVVRSLSEAPKYA 409

Query: 369 RRVAECLGGGRMIR-GRGMRERVWGGAWPVERRRVREFYCFGMETLLRLDLDGTRRFFDG 428
             +AE L      +    +  + W   WP ER+R R F+ FG+  +++ D +G R FF  
Sbjct: 410 SVIAEILREETTKQINSNISRQAWDTLWPPERKRQRAFFLFGLALIVQFDTEGIRSFFRT 469

Query: 429 FFDLDAFYWEGFLSSSLSLSELAMLSLSMF 454
           FF L  + W+GFL S+L+  +L + +L MF
Sbjct: 470 FFRLPKWMWQGFLGSTLTSGDLVLFALYMF 493