MC03g0423 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0423
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	MC03: 11158263 .. 11161753 (+)
RNA-Seq Expression	MC03g0423
Synteny	MC03g0423

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCAATGTGAAATGAAATTAAAGAGGAGGAACAGCCAATTTAGCATAAATTCTAATTAAATAAATGAAAAGATTTTCTTAAATAAAAAAAATACTAAATAAATAAATAAAATAATACAAATAAAAGCTGTCCGGACGATGCGGCTGCACCGTCTCTCGAACGGGCCATGGGCGACAGCTCTCACTGCACACCTATAAAACGCGAACTCGTATGAAATTTAGCTCCCATTTTCTCGCCGGAGATCCTTCAATCACCTGATTACACGGCCGTACCGGCGGCGGAGGGCGGTGGCGCGGCGGTGTTTGGTCGGTTTGGCCTTATCTACAGGCGGTGATGGCTAACTTCCACCGCCTCTTCCGAGTTCTGTTGCTGCTTTTCACTCTTTCAGGTTTGCTTTCTGGATCTTGTTTGTTTGGTTGCTTAGAAACTGCGGGAAATGCTTCGGAACTTGTATTTTGTTTTCTAGTCATGTAATCAGCTACAGAATATGGAGATTCTGACTGGATGAGTTGTATTTTGTGTTGTGAAGTTGAATTCTGTGTTTGTTTCTTGAATTATTCAGAGAGTCGGAAAGGACTCTGTGCTTCTCGCTTTTGTTTTCTTTCTTTTTCTTCCTCTGCGGATTTTTACCGATTAGTTGGACGGAGTTCCGTTTCGGAGCATATTCCGTGTCTTTTAGGACTTTATCTACTCTGGAGAATATTTGAGAAATTGAGATGATCTATTGTCGGTTAGGAATTTGAATTGTTTTGAACTCGGTGAGATTCGTTCTTCTTTTGATTCGATTACCACGAGTTGTTTTCTTTGGAATATGGATTCTCAACACTTGTCTCTGATTTGGTTCCGGTTGTGTTTCATTTCAGGTTCAGCAATTCGAACTCTGAGCGTTGGAATTGGAATTAACTATGGCCAGATCGCTAATAATCTGCCATCTCCATCGCGCGTCGCCATTCTTCTCCAAAGTCTAAATATCAGCAGAGTAAAACTGTACGATGCCGACCCCAATGTCCTGTTCGCCTTCTCCAGAAGTAATGTCAATTTCATCATAGGACTTGGGAATGAATATCTTCAGAGCATGGCCGATCCTCAGAAAGCCCAAGCTTGGGTTCAACAACATGTTCAAACGCACATCACTCAGACTCAAATCACCTGCATCACTGTTGGAAATGAAGTATTCAGCTCTAATGATACCCAATTAAGGTCTAGTCTTCTTCCTGCAATGCAATCTGTTTATAATGCGCTTGTTAATCTTGGGCTGGATAAGCAGGTATCTGTTACCACAGCTCATTCCCTTACCATCTTAGGGAACTCTTTCCCTCCGTCTGCTGGGGTATTCAGGCCAGATCTCGCTGAATACATACAGCCAATTCTCAATTTCCACGCTCAGGTCAAATCGCCTTTTCTTATAAATGCATATCCTTATTTTGCATACAAGGATAACCCTGGCCAAGTTTCTCTAGAGTATGTGCTTTTCCAGCCTAATCAAGGGATGACTGATCCCATTACAAATTTGCACTATGACAATATGCTGTATGCTCAAATCGATGCTGTTTATGCCGCCATCAAAGCGATGGGGCATACAGATATTCAAGTCCAGATTTCGGAGACTGGTTGGCCATCTCGGGGTGATCCAAATGAGGCTGGTGCTACGCCAGAGAACGCCGGGTTGTACAATGGTAATTTGCTAAGAAGAATACAGAATGGACAAGGAACCCCGGCCAAGCCATCAGTTCCTATCGATATCTATGTTTTCGCTCTATTTAATGAGGATCTGAAGCCTGGTCCGGCTTCTGAAAGAAACTATGGTCTTTACTATCCAGATGGTACACCAGTTTATAACATTGGATTACAGGGGTATCTCCCAGAGTTGTTGTATTCATCTTCCAAAAGAAATGTAAGAACCGTTTACTTTGTTTTGTAGTTCCTAATCCTGTTTCTACTTCTTAAATAAAAATCTAATTTGTGAACTATTTCTTTGCAGGTTTTCTCAGTTTTCAGCTTGTTTATGGTATTCATAGCCTGCTTAATCTACAATTAAGAGCATCACCATCAATAATGCGATAGTAGAGGTACACCAAGCAATAGATAGGAATTCTTTTATCAGACACCATGGAAATCATAATTGCCCGAGGAGCTCTATAGAGAAGCACACCCCATACAGATCCTTTCAGCTGCTGCACTTAACTAACCATCTGTATTATATATTTACTTCCCATCCCATCATCAATTTGGTTCATTGGACGATTTTTTTGTAGTTTCAAAAGGCAATAAAGTATTATTATTTCATTAATAAACTCTCTCAATGTCATGATTGGATAATATTGAGCTCCAGAAGAAGGAACAGTTTTCTTTTTCTTCCTCTCTTTGCAGCTCCATTCTGATGATCTGATGTTAAATCTCACGAACTCAAGGACTAACGTAAAGGGCCATTCAATTCATTCTCTGAGAATCTGGAATTGGTATGGTCTCTCTTCTCTCTCCTCTAATGGTATATCCAACATAGAAGAACCTATCTTGTTCATGATAAATTCTGGTAGTCCCCATCACTCTTGTCTCTCTCTCTCCCCTTCATTTTACATGTGAATCTTGCTCTAATTTCCAGTAATCTTCAGCTGTAGCAGCCATATGTGAACATGATTTCATGTAATCTAAACACATATCTGAGCTGAGCTGCCCAATTTGGAACTCCGAAAGTTACCATCTGGGGAAGGTTGGATGGACACATCTAAGATTGTGGACTTTTTCCTTCCTTTGTTCTTATTCCTTGAAGGAACGTAAGTTGGGTATCTGCTTCTGGTGGAGTAATCAAATACGCATTCTCTTTTGACTTTTGACATCTGCTTCTTTGGTGGGGGCTACTGATTTTACCAGATGATTTTTGGTGTTGAATCCATTGAACTGTTGAAGGGTCAGCTTCTCCTTGGCCCTCTGTTTTCTATTGTGATAAGTTTTTATTATTATTATTATTTTATTTTGATGTCTTTTTAGCAGGACTTTGATTTTTATTTTTAAATGGTACCATTGCCATATGAGGAGAAAGAAGAGCCCCATTTCTATATGAGGCGGTGGCTGTCTTTTTCAAAGGGAGGCATGGGAACAGATTTGAGTGGCTTCCAGATAAAGGAATTGAGTATGCTTTTAATTTTTGTGATATTTTCATAAAATAGGAGGAGGTAGTTAAATGGATCTTTTAGGTTTAAAAAGTAGTGATGTAATATCAATGATGAAATATTTTAGTCCACACGTCTTTGTTCGATTTTTGTTTATTTTAGTCTTTGTATCTCCCAACGTCTTTCAATTTTTTCCATTTTAGTCCATGTATTTTCGAAATGTTTATTTTAGTCTATGGACTTTTTGAAAGTAACTAAAATAAATGAGATAGGAATGAAAATGTATCGACCAAACTGACCCCTCTTAAGAGTACAAGAATCAAAAGAAATGTTTCGAAAGTACAAGATTAAAATAAAACTTGAAAGGACAGCGATCAAAAT

mRNA sequence

TGCAATGTGAAATGAAATTAAAGAGGAGGAACAGCCAATTTAGCATAAATTCTAATTAAATAAATGAAAAGATTTTCTTAAATAAAAAAAATACTAAATAAATAAATAAAATAATACAAATAAAAGCTGTCCGGACGATGCGGCTGCACCGTCTCTCGAACGGGCCATGGGCGACAGCTCTCACTGCACACCTATAAAACGCGAACTCGTATGAAATTTAGCTCCCATTTTCTCGCCGGAGATCCTTCAATCACCTGATTACACGGCCGTACCGGCGGCGGAGGGCGGTGGCGCGGCGGTGTTTGGTCGGTTTGGCCTTATCTACAGGCGGTGATGGCTAACTTCCACCGCCTCTTCCGAGTTCTGTTGCTGCTTTTCACTCTTTCAGGTTCAGCAATTCGAACTCTGAGCGTTGGAATTGGAATTAACTATGGCCAGATCGCTAATAATCTGCCATCTCCATCGCGCGTCGCCATTCTTCTCCAAAGTCTAAATATCAGCAGAGTAAAACTGTACGATGCCGACCCCAATGTCCTGTTCGCCTTCTCCAGAAGTAATGTCAATTTCATCATAGGACTTGGGAATGAATATCTTCAGAGCATGGCCGATCCTCAGAAAGCCCAAGCTTGGGTTCAACAACATGTTCAAACGCACATCACTCAGACTCAAATCACCTGCATCACTGTTGGAAATGAAGTATTCAGCTCTAATGATACCCAATTAAGGTCTAGTCTTCTTCCTGCAATGCAATCTGTTTATAATGCGCTTGTTAATCTTGGGCTGGATAAGCAGGTATCTGTTACCACAGCTCATTCCCTTACCATCTTAGGGAACTCTTTCCCTCCGTCTGCTGGGGTATTCAGGCCAGATCTCGCTGAATACATACAGCCAATTCTCAATTTCCACGCTCAGGTCAAATCGCCTTTTCTTATAAATGCATATCCTTATTTTGCATACAAGGATAACCCTGGCCAAGTTTCTCTAGAGTATGTGCTTTTCCAGCCTAATCAAGGGATGACTGATCCCATTACAAATTTGCACTATGACAATATGCTGTATGCTCAAATCGATGCTGTTTATGCCGCCATCAAAGCGATGGGGCATACAGATATTCAAGTCCAGATTTCGGAGACTGGTTGGCCATCTCGGGGTGATCCAAATGAGGCTGGTGCTACGCCAGAGAACGCCGGGTTGTACAATGGTAATTTGCTAAGAAGAATACAGAATGGACAAGGAACCCCGGCCAAGCCATCAGTTCCTATCGATATCTATGTTTTCGCTCTATTTAATGAGGATCTGAAGCCTGGTCCGGCTTCTGAAAGAAACTATGGTCTTTACTATCCAGATGGTACACCAGTTTATAACATTGGATTACAGGGGTATCTCCCAGAGTTGTTGTATTCATCTTCCAAAAGAAATGTTTTCTCAGTTTTCAGCTTGTTTATGGTATTCATAGCCTGCTTAATCTACAATTAAGAGCATCACCATCAATAATGCGATAGTAGAGGTACACCAAGCAATAGATAGGAATTCTTTTATCAGACACCATGGAAATCATAATTGCCCGAGGAGCTCTATAGAGAAGCACACCCCATACAGATCCTTTCAGCTGCTGCACTTAACTAACCATCTGTATTATATATTTACTTCCCATCCCATCATCAATTTGGTTCATTGGACGATTTTTTTGTAGTTTCAAAAGGCAATAAAGTATTATTATTTCATTAATAAACTCTCTCAATGTCATGATTGGATAATATTGAGCTCCAGAAGAAGGAACAGTTTTCTTTTTCTTCCTCTCTTTGCAGCTCCATTCTGATGATCTGATGTTAAATCTCACGAACTCAAGGACTAACGTAAAGGGCCATTCAATTCATTCTCTGAGAATCTGGAATTGGTATGGTCTCTCTTCTCTCTCCTCTAATGGTATATCCAACATAGAAGAACCTATCTTGTTCATGATAAATTCTGCTGTAGCAGCCATATGTGAACATGATTTCATGTAATCTAAACACATATCTGAGCTGAGCTGCCCAATTTGGAACTCCGAAAGTTACCATCTGGGGAAGGTTGGATGGACACATCTAAGATTGTGGACTTTTTCCTTCCTTTGTTCTTATTCCTTGAAGGAACGTAAGTTGGGTATCTGCTTCTGGTGGAGTAATCAAATACGCATTCTCTTTTGACTTTTGACATCTGCTTCTTTGGTGGGGGCTACTGATTTTACCAGATGATTTTTGGTGTTGAATCCATTGAACTGTTGAAGGGACTTTGATTTTTATTTTTAAATGGTACCATTGCCATATGAGGAGAAAGAAGAGCCCCATTTCTATATGAGGCGGTGGCTGTCTTTTTCAAAGGGAGGCATGGGAACAGATTTGAGTGGCTTCCAGATAAAGGAATTGAGTATGCTTTTAATTTTTGTGATATTTTCATAAAATAGGAGGAGGTAGTTAAATGGATCTTTTAGGTTTAAAAAGTAGTGATGTAATATCAATGATGAAATATTTTAGTCCACACGTCTTTGTTCGATTTTTGTTTATTTTAGTCTTTGTATCTCCCAACGTCTTTCAATTTTTTCCATTTTAGTCCATGTATTTTCGAAATGTTTATTTTAGTCTATGGACTTTTTGAAAGTAACTAAAATAAATGAGATAGGAATGAAAATGTATCGACCAAACTGACCCCTCTTAAGAGTACAAGAATCAAAAGAAATGTTTCGAAAGTACAAGATTAAAATAAAACTTGAAAGGACAGCGATCAAAAT

Coding sequence (CDS)

ATGGCTAACTTCCACCGCCTCTTCCGAGTTCTGTTGCTGCTTTTCACTCTTTCAGGTTCAGCAATTCGAACTCTGAGCGTTGGAATTGGAATTAACTATGGCCAGATCGCTAATAATCTGCCATCTCCATCGCGCGTCGCCATTCTTCTCCAAAGTCTAAATATCAGCAGAGTAAAACTGTACGATGCCGACCCCAATGTCCTGTTCGCCTTCTCCAGAAGTAATGTCAATTTCATCATAGGACTTGGGAATGAATATCTTCAGAGCATGGCCGATCCTCAGAAAGCCCAAGCTTGGGTTCAACAACATGTTCAAACGCACATCACTCAGACTCAAATCACCTGCATCACTGTTGGAAATGAAGTATTCAGCTCTAATGATACCCAATTAAGGTCTAGTCTTCTTCCTGCAATGCAATCTGTTTATAATGCGCTTGTTAATCTTGGGCTGGATAAGCAGGTATCTGTTACCACAGCTCATTCCCTTACCATCTTAGGGAACTCTTTCCCTCCGTCTGCTGGGGTATTCAGGCCAGATCTCGCTGAATACATACAGCCAATTCTCAATTTCCACGCTCAGGTCAAATCGCCTTTTCTTATAAATGCATATCCTTATTTTGCATACAAGGATAACCCTGGCCAAGTTTCTCTAGAGTATGTGCTTTTCCAGCCTAATCAAGGGATGACTGATCCCATTACAAATTTGCACTATGACAATATGCTGTATGCTCAAATCGATGCTGTTTATGCCGCCATCAAAGCGATGGGGCATACAGATATTCAAGTCCAGATTTCGGAGACTGGTTGGCCATCTCGGGGTGATCCAAATGAGGCTGGTGCTACGCCAGAGAACGCCGGGTTGTACAATGGTAATTTGCTAAGAAGAATACAGAATGGACAAGGAACCCCGGCCAAGCCATCAGTTCCTATCGATATCTATGTTTTCGCTCTATTTAATGAGGATCTGAAGCCTGGTCCGGCTTCTGAAAGAAACTATGGTCTTTACTATCCAGATGGTACACCAGTTTATAACATTGGATTACAGGGGTATCTCCCAGAGTTGTTGTATTCATCTTCCAAAAGAAATGTTTTCTCAGTTTTCAGCTTGTTTATGGTATTCATAGCCTGCTTAATCTACAATTAA

Protein sequence

MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSKRNVFSVFSLFMVFIACLIYN

Homology

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BLAST of MC03g0423 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 520.8 bits (1340), Expect = 1.3e-146
Identity = 250/372 (67.20%), Postives = 313/372 (84.14%), Query Frame = 0


Query: 8   FRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNV 67
           FRVLLLLF      I+    G+GINYGQIANNLPSP+RVA+LL+SLNI+RVKLYDADPNV
Sbjct: 10  FRVLLLLFLTLSERIK--GQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNV 69

Query: 68  LFAFSRSNVNFIIGLGNEYLQSMA-DPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSN 127
           LF+FS S V+F+IGLGNEYLQ+M+ DP KAQ W+QQ ++ HI++T+IT I VGNE+F +N
Sbjct: 70  LFSFSNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTN 129

Query: 128 DTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQP 187
           D  L  SLLPAM+SVY AL NLGL+KQV+VT+AHSL IL  S+PPS+G F+ +  +Y+QP
Sbjct: 130 DHVLIQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQP 189

Query: 188 ILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQID 247
           +L+FH+Q++SPFLINAYP+FAYKD+P +V LEYVLFQPNQGM DP TNLHYDNML+AQ+D
Sbjct: 190 LLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVD 249

Query: 248 AVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAKP 307
           A+Y+AIK +GHTDI+V+ISETGWPS+GD NE GA+PENA LYNGNLL+ IQ  +GTPAK 
Sbjct: 250 ALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQ 309

Query: 308 SVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSKRNVFSV 367
           SVPID+YVFALFNE+LKPGP SERNYGL+YPDG PVYN+G+QGYLP+++Y +S+     +
Sbjct: 310 SVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIY-TSRATTIKI 369

Query: 368 FSLFMVFIACLI 379
            +L+ V +   +
Sbjct: 370 LNLWRVVMGLAV 378

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BLAST of MC03g0423 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 4.9e-117
Identity = 202/348 (58.05%), Postives = 264/348 (75.86%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           + +FHR    LL L +L+   + T +  IG+NYGQI +NLPSP+ V  L++S+  ++VKL
Sbjct: 3   LTSFHR--SSLLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKL 62

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDA+P +L AFS + + FIIGLGNEYL  M DP KA  W++Q+V   +  T ITCIT+GN
Sbjct: 63  YDANPQILKAFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGN 122

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           E+ + ND+ L ++LLPAMQ V++AL+  GL  Q+SVTTAHSL+IL +SFPPSAG F+PDL
Sbjct: 123 EILALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDL 182

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            + + PIL FH +  SPFLINAYP+FAYK NP +V L++VLFQPNQG+ DP T  HYDNM
Sbjct: 183 LDSLTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNM 242

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           L+AQIDAVY+A+ A G   ++V+ISETGWPS+GD +E GATPENA  YNGNL++ + +G+
Sbjct: 243 LFAQIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGK 302

Query: 301 --GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIG 347
              TP KP+  + IYVFALFNE+LKPGP SERNYGL+ PDGT  Y++G
Sbjct: 303 KTKTPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLG 348

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BLAST of MC03g0423 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.5e-107
Identity = 190/341 (55.72%), Postives = 251/341 (73.61%), Query Frame = 0


Query: 11  LLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNVLFA 70
           L  LF L+  ++  +   IGINYGQ+ANNLP P  V  LL+S+  ++VKLYDADP  L A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 71  FSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSNDTQL 130
           F+ S     + LGNEYL  M+DP KAQ WV+++VQ ++  T+I  I VGNEV +SN + L
Sbjct: 69  FAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSAL 128

Query: 131 RSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQPILNF 190
            ++L PAMQS++ ALV+ GL+KQ+ VTTAHSL IL  S+PPSA  FR DL   + PIL+F
Sbjct: 129 TAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILDF 188

Query: 191 HAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQIDAVYA 250
           H +  SP LINAYP+FAY++NP  VSL++VLFQPNQG TDP +N HYDNML+AQ+DAVY 
Sbjct: 189 HVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYH 248

Query: 251 AIKAMG--HTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ-GTPAKPS 310
           A+ A+G  +  + + +SETGWPS GDP E GAT +NA  YNGNL++ + + +  TP +P 
Sbjct: 249 ALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRPE 308

Query: 311 VPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQ 349
             + I+VFALFNE++KPGP SERNYGL+ PDGTPVY++G++
Sbjct: 309 CDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIK 349

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BLAST of MC03g0423 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 1.6e-80
Identity = 147/320 (45.94%), Postives = 213/320 (66.56%), Query Frame = 0


Query: 29  IGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNVLFAFSRSNVNFIIGLGNEYLQ 88
           IG+NYGQ+A+NLP PS    LLQS +I +V+LY ADP ++ A + + V  +IG  N  + 
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVP 85

Query: 89  SMA-DPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSNDTQLRSSLLPAMQSVYNALVN 148
           S+A DP  A  W+  +V      ++I  ITVGNE+  SND  L + LLPAMQ+V  AL  
Sbjct: 86  SLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEA 145

Query: 149 LGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQPILNFHAQVKSPFLINAYPYFA 208
           + L  ++ V+T +S+T+LG+S PPS+G F       ++ IL F +   SPF IN YP+FA
Sbjct: 146 VSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFA 205

Query: 209 YKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQIDAVYAAIKAMGHTDIQVQISET 268
           Y+ +P   +L + LF+PN G  D  T + Y NM  AQ+DAV++A+K+MG   +++ ++ET
Sbjct: 206 YQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAET 265

Query: 269 GWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAKPSVPIDIYVFALFNEDLKPGPA 328
           GW SRGD NE GA+ +NA  YNGNL+  +++  GTP  P  P+D Y+FAL++E+LKPGP+
Sbjct: 266 GWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPS 325

Query: 329 SERNYGLYYPDGTPVYNIGL 348
           SER +GL+  D + VY++GL
Sbjct: 326 SERAFGLFKTDLSMVYDVGL 345

[Close]

BLAST of MC03g0423 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.0e-78
Identity = 151/344 (43.90%), Postives = 225/344 (65.41%), Query Frame = 0


Query: 11  LLLLFTLSGSAIRTLSVG----IGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPN 70
           L L+F +  S +  L+ G    IGI YG+ A+NLPSP+RV+ L+Q LNI  V++YDA+ +
Sbjct: 5   LNLVFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANID 64

Query: 71  VLFAFSRSNVNFIIGLGNEYLQSMADPQ-KAQAWVQQHVQTHITQTQITCITVGNEVFSS 130
           VL AF+ + +  +IG+ N  L + A  Q     W+  ++  +   T+IT I+VG EV  +
Sbjct: 65  VLKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEA 124

Query: 131 NDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQ 190
            D      +LPAM++++ AL   GLDK++ ++++HSL IL  SFPPS+  F    + +++
Sbjct: 125 PD-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLK 184

Query: 191 PILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQI 250
           P+L F  + +SPF+I+ YPY+AY+D+  +V LEY LF+ +  + DP T L Y NM  AQ+
Sbjct: 185 PMLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQL 244

Query: 251 DAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAK 310
           DA+Y A+ AM    ++V ++E+GWPS+G P E  ATPENA  YN NL+R +    GTPAK
Sbjct: 245 DAIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAK 304

Query: 311 PSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQG 350
           P   ID+Y+F+LFNE+ KPG  SERN+G++Y +GT VY +   G
Sbjct: 305 PGEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTG 347

[Close]

BLAST of MC03g0423 vs. NCBI nr
Match: XP_022142551.1 (glucan endo-1,3-beta-glucosidase 14 [Momordica charantia])

HSP 1 Score: 752 bits (1941), Expect = 9.62e-274
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN
Sbjct: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL
Sbjct: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
           AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ
Sbjct: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVFSLFMVFIACLIYN
Sbjct: 361 RNVFSVFSLFMVFIACLIYN 380

[Close]

BLAST of MC03g0423 vs. NCBI nr
Match: XP_008461417.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 14 [Cucumis melo])

HSP 1 Score: 696 bits (1797), Expect = 8.51e-252
Identity = 347/380 (91.32%), Postives = 365/380 (96.05%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLL+LFT SGS IRTLSVG+GINYGQIANNLPSPSRVAILLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLMLFTFSGSVIRTLSVGVGINYGQIANNLPSPSRVAILLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVLFAFSRS VNFIIGLGNEYLQ+M+DPQKA AWVQQHVQTHI+QT+ITCITVGN
Sbjct: 61  YDADPNVLFAFSRSEVNFIIGLGNEYLQNMSDPQKALAWVQQHVQTHISQTKITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF+SND+QLRS+LLPAMQSVYNALVNLGLDKQVSVTTAHSL ILGNSFPPSAG F+PDL
Sbjct: 121 EVFNSNDSQLRSNLLPAMQSVYNALVNLGLDKQVSVTTAHSLNILGNSFPPSAGTFKPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
           AEY+QPILNFH+ VKSPFLINAYP+FAYKDNP QV LEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 AEYLQPILNFHSMVKSPFLINAYPFFAYKDNPTQVPLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKAMGHTDI+VQISETGWPSRGDPNE GATPENAGLYNGNLLRRI++GQ
Sbjct: 241 LYAQIDAVYAAIKAMGHTDIRVQISETGWPSRGDPNEVGATPENAGLYNGNLLRRIESGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSV SL ++FIA  IY 
Sbjct: 361 RNVFSVLSLLLIFIAYGIYT 380

[Close]

BLAST of MC03g0423 vs. NCBI nr
Match: XP_038893871.1 (glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida])

HSP 1 Score: 695 bits (1794), Expect = 2.44e-251
Identity = 344/380 (90.53%), Postives = 365/380 (96.05%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFH  FRVLL+LFT SGS IRTLSVGIGIN+GQIANNLPSPSRVA+LLQSLNISRVKL
Sbjct: 1   MANFHHFFRVLLMLFTFSGSVIRTLSVGIGINFGQIANNLPSPSRVAVLLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVL AFS+SNVNFIIGLGNEYLQ+M+DPQKA AWVQQHVQTHI+QTQITCITVGN
Sbjct: 61  YDADPNVLLAFSKSNVNFIIGLGNEYLQNMSDPQKAIAWVQQHVQTHISQTQITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF+SND QLRS+LLPAMQSVYNALVNLGLDKQVSVTTAHSL ILGNSFPPSAG F+PDL
Sbjct: 121 EVFNSNDAQLRSNLLPAMQSVYNALVNLGLDKQVSVTTAHSLNILGNSFPPSAGAFKPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            EYIQPILNFH+ VKSPFLINAYP+FAYK+NPGQVSLEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 VEYIQPILNFHSMVKSPFLINAYPFFAYKENPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKAMGHTDI+VQISETGWPSRGD NE GATPENAGLYNGNLLRRI++GQ
Sbjct: 241 LYAQIDAVYAAIKAMGHTDIRVQISETGWPSRGDLNEVGATPENAGLYNGNLLRRIESGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVY++GLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYDVGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVF+LF++FIAC IY 
Sbjct: 361 RNVFSVFNLFLIFIACGIYT 380

[Close]

BLAST of MC03g0423 vs. NCBI nr
Match: KAG6573623.1 (Glucan endo-1,3-beta-glucosidase 14, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012704.1 Glucan endo-1,3-beta-glucosidase 14 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694 bits (1792), Expect = 4.92e-251
Identity = 347/380 (91.32%), Postives = 362/380 (95.26%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLLLLFTLS S IRTLSVGIGINYGQIANNLPSPSRV  LLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLLLFTLSDSVIRTLSVGIGINYGQIANNLPSPSRVVTLLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVL+AFS SNVNFIIGLGNEYLQ+M DPQKA AWVQQHVQTHI+QTQITCITVGN
Sbjct: 61  YDADPNVLYAFSSSNVNFIIGLGNEYLQNMTDPQKALAWVQQHVQTHISQTQITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF SND+QLRSSLLPAMQSV+NALVNLGLDKQVSVTTAHSL IL NSFPPSAG FRPDL
Sbjct: 121 EVFVSNDSQLRSSLLPAMQSVHNALVNLGLDKQVSVTTAHSLNILANSFPPSAGSFRPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            EY+QPILNFH+  KSPFLINAYP+FAYKDNPGQV LEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 TEYLQPILNFHSMAKSPFLINAYPFFAYKDNPGQVPLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKA+GHTDIQVQISETGWPSRGDPNE GATPENAGLYNGNLLRRIQ+GQ
Sbjct: 241 LYAQIDAVYAAIKALGHTDIQVQISETGWPSRGDPNEVGATPENAGLYNGNLLRRIQSGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPS+PIDIYVFALFNE+LKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSIPIDIYVFALFNENLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVFSL ++FIA LIY+
Sbjct: 361 RNVFSVFSLLLIFIASLIYS 380

[Close]

BLAST of MC03g0423 vs. NCBI nr
Match: XP_023542405.1 (glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691 bits (1783), Expect = 1.16e-249
Identity = 344/380 (90.53%), Postives = 361/380 (95.00%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLLLLFTLS S IRTLSVGIGINYGQIANNLPSPSRV  LLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLLLFTLSDSVIRTLSVGIGINYGQIANNLPSPSRVVTLLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVL+AFS SNVNFIIGLGNEYLQ+M DPQKA AWVQQHVQTHI+QTQITCITVGN
Sbjct: 61  YDADPNVLYAFSSSNVNFIIGLGNEYLQNMTDPQKALAWVQQHVQTHISQTQITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF SND+QLRS+LLPAMQSV+NALVNLGLDKQVSVTTAHSL IL NSFPPSAG FRPDL
Sbjct: 121 EVFVSNDSQLRSNLLPAMQSVHNALVNLGLDKQVSVTTAHSLNILANSFPPSAGSFRPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            EY+QPILNFH+  KSPFLINAYP+FAYKDNPGQV LEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 TEYLQPILNFHSMAKSPFLINAYPFFAYKDNPGQVPLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKA+GHTDIQVQISETGWPSRGDPNE GATPENAGLYNGNLLRRIQ+GQ
Sbjct: 241 LYAQIDAVYAAIKALGHTDIQVQISETGWPSRGDPNEVGATPENAGLYNGNLLRRIQSGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPS+PIDIYVFALFNE+LKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSIPIDIYVFALFNENLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVFSL ++F+  LIY+
Sbjct: 361 RNVFSVFSLLLIFLGSLIYS 380

[Close]

BLAST of MC03g0423 vs. ExPASy TrEMBL
Match: A0A6J1CMI6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012635 PE=3 SV=1)

HSP 1 Score: 752 bits (1941), Expect = 4.66e-274
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN
Sbjct: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL
Sbjct: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
           AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ
Sbjct: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVFSLFMVFIACLIYN
Sbjct: 361 RNVFSVFSLFMVFIACLIYN 380

[Close]

BLAST of MC03g0423 vs. ExPASy TrEMBL
Match: A0A1S3CEC3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500010 PE=3 SV=1)

HSP 1 Score: 696 bits (1797), Expect = 4.12e-252
Identity = 347/380 (91.32%), Postives = 365/380 (96.05%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLL+LFT SGS IRTLSVG+GINYGQIANNLPSPSRVAILLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLMLFTFSGSVIRTLSVGVGINYGQIANNLPSPSRVAILLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVLFAFSRS VNFIIGLGNEYLQ+M+DPQKA AWVQQHVQTHI+QT+ITCITVGN
Sbjct: 61  YDADPNVLFAFSRSEVNFIIGLGNEYLQNMSDPQKALAWVQQHVQTHISQTKITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF+SND+QLRS+LLPAMQSVYNALVNLGLDKQVSVTTAHSL ILGNSFPPSAG F+PDL
Sbjct: 121 EVFNSNDSQLRSNLLPAMQSVYNALVNLGLDKQVSVTTAHSLNILGNSFPPSAGTFKPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
           AEY+QPILNFH+ VKSPFLINAYP+FAYKDNP QV LEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 AEYLQPILNFHSMVKSPFLINAYPFFAYKDNPTQVPLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKAMGHTDI+VQISETGWPSRGDPNE GATPENAGLYNGNLLRRI++GQ
Sbjct: 241 LYAQIDAVYAAIKAMGHTDIRVQISETGWPSRGDPNEVGATPENAGLYNGNLLRRIESGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSV SL ++FIA  IY 
Sbjct: 361 RNVFSVLSLLLIFIAYGIYT 380

[Close]

BLAST of MC03g0423 vs. ExPASy TrEMBL
Match: A0A6J1G0Y8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449677 PE=3 SV=1)

HSP 1 Score: 688 bits (1775), Expect = 9.27e-249
Identity = 344/380 (90.53%), Postives = 360/380 (94.74%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLLLLFTLS S IRTLSVGIGINYGQIANNLPSPSRV  LLQSLNISRVKL
Sbjct: 1   MANFHRLFRVLLLLFTLSDSVIRTLSVGIGINYGQIANNLPSPSRVVTLLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVL+AFS SNVNFIIGLGNEYLQ+M DPQKA AWVQQHVQTHI+QTQITCITVGN
Sbjct: 61  YDADPNVLYAFSSSNVNFIIGLGNEYLQNMTDPQKALAWVQQHVQTHISQTQITCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF SND+QLRS+LLPAMQSV+NALVNLGLDKQVSVTTAHSL IL NSFPPSAG FRPDL
Sbjct: 121 EVFVSNDSQLRSNLLPAMQSVHNALVNLGLDKQVSVTTAHSLNILANSFPPSAGSFRPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            EY+QPILNFH+  KSPFLINAYP+FAYKDNPGQV LEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 181 TEYLQPILNFHSMAKSPFLINAYPFFAYKDNPGQVPLEYVLFQPNQGMTDPITNLHYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKA+GHTDIQVQISETGWPSRGD NE GATPENAGLYNGNLLRRIQ+GQ
Sbjct: 241 LYAQIDAVYAAIKALGHTDIQVQISETGWPSRGDLNEVGATPENAGLYNGNLLRRIQSGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPS+PIDIYVFALFNE+LKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSIPIDIYVFALFNENLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVFSL ++FIA  IY+
Sbjct: 361 RNVFSVFSLLLIFIASFIYS 380

[Close]

BLAST of MC03g0423 vs. ExPASy TrEMBL
Match: A0A0A0LVS8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G164660 PE=3 SV=1)

HSP 1 Score: 686 bits (1769), Expect = 3.50e-247
Identity = 340/380 (89.47%), Postives = 361/380 (95.00%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MANFHRLFRVLL+LF LSGS I TLSVG+GINYGQIANNLPSPSRVA LL+SLNISRVKL
Sbjct: 42  MANFHRLFRVLLMLFALSGSVIPTLSVGVGINYGQIANNLPSPSRVASLLRSLNISRVKL 101

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVLFAFSRS VNFIIGLGNEYLQ+M+DPQKA AWVQQHVQTHI+QT+ITCITVGN
Sbjct: 102 YDADPNVLFAFSRSEVNFIIGLGNEYLQNMSDPQKALAWVQQHVQTHISQTKITCITVGN 161

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF+SND QLRS+LLPAMQSVYN LVNLGLDKQVSVTTAHSL ILGNSFPPSAG F+PDL
Sbjct: 162 EVFNSNDNQLRSNLLPAMQSVYNVLVNLGLDKQVSVTTAHSLNILGNSFPPSAGTFKPDL 221

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
           AEY+QPILNFH+ VKSPFLINAYP+FAYKDNP QVSLEYVLFQPNQGMTDPITNLHYDNM
Sbjct: 222 AEYLQPILNFHSMVKSPFLINAYPFFAYKDNPTQVSLEYVLFQPNQGMTDPITNLHYDNM 281

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKAMGHTDI+VQISETGWPSRGDPNE GATPENAGLYNGNLLRRI++GQ
Sbjct: 282 LYAQIDAVYAAIKAMGHTDIRVQISETGWPSRGDPNEVGATPENAGLYNGNLLRRIESGQ 341

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTP KPS+PIDIYVFALFNEDLKPGP+SERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 342 GTPLKPSIPIDIYVFALFNEDLKPGPSSERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 401

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSV SL ++F+A  IY 
Sbjct: 402 RNVFSVLSLLLIFVAYGIYT 421

[Close]

BLAST of MC03g0423 vs. ExPASy TrEMBL
Match: A0A6J1HPS8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111466594 PE=3 SV=1)

HSP 1 Score: 684 bits (1764), Expect = 4.40e-247
Identity = 341/380 (89.74%), Postives = 359/380 (94.47%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           MAN HRLFRVLLLLFTLS S IRTLSVGIGINYGQIANNLPSPSRV  LLQSLNISRVKL
Sbjct: 1   MANVHRLFRVLLLLFTLSDSVIRTLSVGIGINYGQIANNLPSPSRVVTLLQSLNISRVKL 60

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDADPNVL+AFS SNVNFIIGLGNEYLQ+M DPQKA AWVQQHVQTHI+QTQI+CITVGN
Sbjct: 61  YDADPNVLYAFSSSNVNFIIGLGNEYLQNMTDPQKALAWVQQHVQTHISQTQISCITVGN 120

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           EVF SND+QLRS+LLPAMQSV+NALVNLGLDKQVSVTTAHSL IL NSFPPSAG FRPDL
Sbjct: 121 EVFVSNDSQLRSNLLPAMQSVHNALVNLGLDKQVSVTTAHSLNILANSFPPSAGSFRPDL 180

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            EY+QPILNFH+  KSPFLINAYP+FAYKDNPGQV LEYVLFQPNQGMTDPITNL YDNM
Sbjct: 181 TEYLQPILNFHSMAKSPFLINAYPFFAYKDNPGQVPLEYVLFQPNQGMTDPITNLQYDNM 240

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           LYAQIDAVYAAIKA+GHTDIQVQISETGWPSRGDPNE GATPENAGLYNGNLLRRIQ+GQ
Sbjct: 241 LYAQIDAVYAAIKALGHTDIQVQISETGWPSRGDPNEVGATPENAGLYNGNLLRRIQSGQ 300

Query: 301 GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360
           GTPAKPS+PIDIYVFALFNE+LKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK
Sbjct: 301 GTPAKPSIPIDIYVFALFNENLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSK 360

Query: 361 RNVFSVFSLFMVFIACLIYN 380
           RNVFSVFSL ++F+  LIY+
Sbjct: 361 RNVFSVFSLLLIFLVSLIYS 380

[Close]

BLAST of MC03g0423 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 522.7 bits (1345), Expect = 2.5e-148
Identity = 248/352 (70.45%), Postives = 305/352 (86.65%), Query Frame = 0


Query: 8   FRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNV 67
           FRVLLLLF      I+    G+GINYGQIANNLPSP+RVA+LL+SLNI+RVKLYDADPNV
Sbjct: 10  FRVLLLLFLTLSERIK--GQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNV 69

Query: 68  LFAFSRSNVNFIIGLGNEYLQSMA-DPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSN 127
           LF+FS S V+F+IGLGNEYLQ+M+ DP KAQ W+QQ ++ HI++T+IT I VGNE+F +N
Sbjct: 70  LFSFSNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTN 129

Query: 128 DTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQP 187
           D  L  SLLPAM+SVY AL NLGL+KQV+VT+AHSL IL  S+PPS+G F+ +  +Y+QP
Sbjct: 130 DHVLIQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQP 189

Query: 188 ILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQID 247
           +L+FH+Q++SPFLINAYP+FAYKD+P +V LEYVLFQPNQGM DP TNLHYDNML+AQ+D
Sbjct: 190 LLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVD 249

Query: 248 AVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAKP 307
           A+Y+AIK +GHTDI+V+ISETGWPS+GD NE GA+PENA LYNGNLL+ IQ  +GTPAK 
Sbjct: 250 ALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQ 309

Query: 308 SVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSS 359
           SVPID+YVFALFNE+LKPGP SERNYGL+YPDG PVYN+G+QGYLP+++Y+S
Sbjct: 310 SVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIYTS 359

[Close]

BLAST of MC03g0423 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 520.8 bits (1340), Expect = 9.4e-148
Identity = 250/372 (67.20%), Postives = 313/372 (84.14%), Query Frame = 0


Query: 8   FRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNV 67
           FRVLLLLF      I+    G+GINYGQIANNLPSP+RVA+LL+SLNI+RVKLYDADPNV
Sbjct: 10  FRVLLLLFLTLSERIK--GQGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNV 69

Query: 68  LFAFSRSNVNFIIGLGNEYLQSMA-DPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSN 127
           LF+FS S V+F+IGLGNEYLQ+M+ DP KAQ W+QQ ++ HI++T+IT I VGNE+F +N
Sbjct: 70  LFSFSNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTN 129

Query: 128 DTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQP 187
           D  L  SLLPAM+SVY AL NLGL+KQV+VT+AHSL IL  S+PPS+G F+ +  +Y+QP
Sbjct: 130 DHVLIQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQP 189

Query: 188 ILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQID 247
           +L+FH+Q++SPFLINAYP+FAYKD+P +V LEYVLFQPNQGM DP TNLHYDNML+AQ+D
Sbjct: 190 LLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVD 249

Query: 248 AVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAKP 307
           A+Y+AIK +GHTDI+V+ISETGWPS+GD NE GA+PENA LYNGNLL+ IQ  +GTPAK 
Sbjct: 250 ALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQ 309

Query: 308 SVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSKRNVFSV 367
           SVPID+YVFALFNE+LKPGP SERNYGL+YPDG PVYN+G+QGYLP+++Y +S+     +
Sbjct: 310 SVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIY-TSRATTIKI 369

Query: 368 FSLFMVFIACLI 379
            +L+ V +   +
Sbjct: 370 LNLWRVVMGLAV 378

[Close]

BLAST of MC03g0423 vs. TAIR 10
Match: AT2G27500.3 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 427.6 bits (1098), Expect = 1.1e-119
Identity = 199/301 (66.11%), Postives = 254/301 (84.39%), Query Frame = 0


Query: 79  IIGLGNEYLQSMA-DPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSNDTQLRSSLLPA 138
           +IGLGNEYLQ+M+ DP KAQ W+QQ ++ HI++T+IT I VGNE+F +ND  L  SLLPA
Sbjct: 1   MIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPA 60

Query: 139 MQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQPILNFHAQVKSP 198
           M+SVY AL NLGL+KQV+VT+AHSL IL  S+PPS+G F+ +  +Y+QP+L+FH+Q++SP
Sbjct: 61  MKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESP 120

Query: 199 FLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQIDAVYAAIKAMGH 258
           FLINAYP+FAYKD+P +V LEYVLFQPNQGM DP TNLHYDNML+AQ+DA+Y+AIK +GH
Sbjct: 121 FLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGH 180

Query: 259 TDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQGTPAKPSVPIDIYVFAL 318
           TDI+V+ISETGWPS+GD NE GA+PENA LYNGNLL+ IQ  +GTPAK SVPID+YVFAL
Sbjct: 181 TDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFAL 240

Query: 319 FNEDLKPGPASERNYGLYYPDGTPVYNIGLQGYLPELLYSSSKRNVFSVFSLFMVFIACL 378
           FNE+LKPGP SERNYGL+YPDG PVYN+G+QGYLP+++Y +S+     + +L+ V +   
Sbjct: 241 FNENLKPGPVSERNYGLFYPDGKPVYNVGMQGYLPDIIY-TSRATTIKILNLWRVVMGLA 300

[Close]

BLAST of MC03g0423 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 422.5 bits (1085), Expect = 3.5e-118
Identity = 202/348 (58.05%), Postives = 264/348 (75.86%), Query Frame = 0


Query: 1   MANFHRLFRVLLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKL 60
           + +FHR    LL L +L+   + T +  IG+NYGQI +NLPSP+ V  L++S+  ++VKL
Sbjct: 3   LTSFHR--SSLLFLISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKL 62

Query: 61  YDADPNVLFAFSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGN 120
           YDA+P +L AFS + + FIIGLGNEYL  M DP KA  W++Q+V   +  T ITCIT+GN
Sbjct: 63  YDANPQILKAFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGN 122

Query: 121 EVFSSNDTQLRSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDL 180
           E+ + ND+ L ++LLPAMQ V++AL+  GL  Q+SVTTAHSL+IL +SFPPSAG F+PDL
Sbjct: 123 EILALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDL 182

Query: 181 AEYIQPILNFHAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNM 240
            + + PIL FH +  SPFLINAYP+FAYK NP +V L++VLFQPNQG+ DP T  HYDNM
Sbjct: 183 LDSLTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNM 242

Query: 241 LYAQIDAVYAAIKAMGHTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ 300
           L+AQIDAVY+A+ A G   ++V+ISETGWPS+GD +E GATPENA  YNGNL++ + +G+
Sbjct: 243 LFAQIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGK 302

Query: 301 --GTPAKPSVPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIG 347
              TP KP+  + IYVFALFNE+LKPGP SERNYGL+ PDGT  Y++G
Sbjct: 303 KTKTPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLG 348

[Close]

BLAST of MC03g0423 vs. TAIR 10
Match: AT5G42100.1 (beta-1,3-glucanase_putative )

HSP 1 Score: 389.8 bits (1000), Expect = 2.5e-108
Identity = 190/341 (55.72%), Postives = 251/341 (73.61%), Query Frame = 0


Query: 11  LLLLFTLSGSAIRTLSVGIGINYGQIANNLPSPSRVAILLQSLNISRVKLYDADPNVLFA 70
           L  LF L+  ++  +   IGINYGQ+ANNLP P  V  LL+S+  ++VKLYDADP  L A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 71  FSRSNVNFIIGLGNEYLQSMADPQKAQAWVQQHVQTHITQTQITCITVGNEVFSSNDTQL 130
           F+ S     + LGNEYL  M+DP KAQ WV+++VQ ++  T+I  I VGNEV +SN + L
Sbjct: 69  FAGSGFELTVALGNEYLAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSAL 128

Query: 131 RSSLLPAMQSVYNALVNLGLDKQVSVTTAHSLTILGNSFPPSAGVFRPDLAEYIQPILNF 190
            ++L PAMQS++ ALV+ GL+KQ+ VTTAHSL IL  S+PPSA  FR DL   + PIL+F
Sbjct: 129 TAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILDF 188

Query: 191 HAQVKSPFLINAYPYFAYKDNPGQVSLEYVLFQPNQGMTDPITNLHYDNMLYAQIDAVYA 250
           H +  SP LINAYP+FAY++NP  VSL++VLFQPNQG TDP +N HYDNML+AQ+DAVY 
Sbjct: 189 HVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYH 248

Query: 251 AIKAMG--HTDIQVQISETGWPSRGDPNEAGATPENAGLYNGNLLRRIQNGQ-GTPAKPS 310
           A+ A+G  +  + + +SETGWPS GDP E GAT +NA  YNGNL++ + + +  TP +P 
Sbjct: 249 ALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRPE 308

Query: 311 VPIDIYVFALFNEDLKPGPASERNYGLYYPDGTPVYNIGLQ 349
             + I+VFALFNE++KPGP SERNYGL+ PDGTPVY++G++
Sbjct: 309 CDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIK 349

The following BLAST results are available for this feature:

BLAST of MC03g0423 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQG9	1.3e-146	67.20	Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500...	[more]
Q8L868	4.9e-117	58.05	Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860...	[more]
Q9FHX5	3.5e-107	55.72	Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100...	[more]
Q9M069	1.6e-80	45.94	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]
Q8VYE5	2.0e-78	43.90	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of MC03g0423 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142551.1	9.62e-274	100.00	glucan endo-1,3-beta-glucosidase 14 [Momordica charantia]	[more]
XP_008461417.1	8.51e-252	91.32	PREDICTED: glucan endo-1,3-beta-glucosidase 14 [Cucumis melo]	[more]
XP_038893871.1	2.44e-251	90.53	glucan endo-1,3-beta-glucosidase 14-like [Benincasa hispida]	[more]
KAG6573623.1	4.92e-251	91.32	Glucan endo-1,3-beta-glucosidase 14, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023542405.1	1.16e-249	90.53	glucan endo-1,3-beta-glucosidase 14 isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MC03g0423 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMI6	4.66e-274	100.00	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012635 ...	[more]
A0A1S3CEC3	4.12e-252	91.32	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103500010 PE=3 SV...	[more]
A0A6J1G0Y8	9.27e-249	90.53	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111449677 P...	[more]
A0A0A0LVS8	3.50e-247	89.47	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G164660 PE=3...	[more]
A0A6J1HPS8	4.40e-247	89.74	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111466594 PE=...	[more]

BLAST of MC03g0423 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27500.2	2.5e-148	70.45	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.1	9.4e-148	67.20	Glycosyl hydrolase superfamily protein	[more]
AT2G27500.3	1.1e-119	66.11	Glycosyl hydrolase superfamily protein	[more]
AT1G32860.1	3.5e-118	58.05	Glycosyl hydrolase superfamily protein	[more]
AT5G42100.1	2.5e-108	55.72	beta-1,3-glucanase_putative	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 28..351 e-value: 1.6E-126 score: 423.6
None	No IPR available	PANTHER	PTHR32227:SF236	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 14	coord: 8..364
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 29..345 e-value: 4.5E-92 score: 309.0
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 8..364
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 29..345

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0423.1	MC03g0423.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds