MC03g0378 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0378
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionpheophorbide a oxygenase, chloroplastic
LocationMC03: 10535048 .. 10539502 (+)
RNA-Seq ExpressionMC03g0378
SyntenyMC03g0378
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTTTCGATCGCTACCTCTGCTTCTTCTGCTACTCTTATTACCTCTTCGTTATATGATGTGAAATCTCGCAGAATAGTCCCCTTTGTAATCGCCGCGGATCGCAGCCCTAATTTTCTGGCGAGAAAATTGCCTAGTAGGGCAGCAGCTCTCAGGCCGGTTCGAGTAGCTGCGCCTCCAACGCCGCCCTCGACGGCAGATTCCGAGAAATCTGCGGTAGAGAATTCCGATGAAGTGGAAGTGGATGAGTCGGGCAATGAGAGCTCGTCTTCGCCGTTTTCTTGGAGAGATAATTGGTACCCTGTTTCCCTAATTGAAGATTTGGATCCTGCTTTGCCAACCCCGTTTCAGCTTCTGGGTAGAGATATCGTTCTTTGGTTTGATAAATCGAGAGGCGAGTGGGTCGCTTTTGATGATAAATGCCCTCACCGGCTTGCCCCTTTATCTGTAAGCTTCCTGAATTGTTCTTCGTCTTCCATATTTAGCATTGCGAAGCTCCTGTTTTCTGATTCCATGGGAAAATTAAAGAATGTAATTCATTGAGTTCTCTTTGTTTTCTCTTGCACATGAATTTGGTTTTTGTGAAAGGAAAGTGCAACAAGTTATGGGTTGATAAAATGGGTACATTCTGGTCATCTAGCTGCTATAAGGTTCAAAAATTGAGTTCAGTTGGAACCCTCTAGTCTCTCTGATGAATTGGAGTGTAGCTCTGAGTTTGTGATGGTGAAAAGAAATGAATGCAAAAATCACTTACTAGTTGTTAAAATTGCACGTTCAAACTTTCAATTTGAGGCATTTATAATTCTGTTAGTTGCAACTTAAAAAATACCCAAACGGACTATCCAAAAAAAACAAAAAAGAGTAAAATAAGAATTGTTACGAATATACCAGCCTAGATTCTAAACCCTAGAATCTCCCTAACTTCAATGAGATGGTGTAATTTTTTAGGAAGGGCGGATAGATGAAGGTGGCAACTTGCAGTGCTCATACCATGGATGGTCCTTCGATGGGTGTGGATCTTGTGTTAAAATCCCTCAGGCATCATCAGAAGGCCCAGAATCTCGGGCTCATCAGTCGCCACGAGCATGTGCTACAAGGTTCCCTACTCTGGTCTCCCAAGGCCTTCTCTTTGTTTGGCCTGATGAGAACGGTTGGGAAAGAGTCAATGCCACAGCACCTCCAAGGTACATGATTCAGAATTTGAATCTCCATTTTTTTAACCTTCTTGATCAGAAATTTTCACAATGAATAAGCTTCATTCCCTTTCCATCTATAATTTAATTGCAATATGCAGTCAATCCTTCATTATCACTGCTATTGTCTAGGCTCCCTGATGACTTCGATAAGCCTGAGTTCTCATCCGTTACGATTCAGCGGGATCTATTTTATGGTTATGATACACTAATGGAGAATGTCTCAGATCCTTCCCACATTGATTTTGCTCATCACAAGGTAAGGGAAAATGCCAAAGAGAGAGAAACCTGTTTCTATTTTACTTGAGTTTGGCATCGGTGAACAGTTATAGATTGAATCATTATGCTAGCTTTTGTTTTACTGTTATTTCTGGTTCTGCTGGATGCTTAGTCCAATGAAATGTTTCACATATAAAGTACAAAAGGCTTGGATGGTACCTACTCTGTGCTGTTAGATAGTTAGTTATTTACTACGTTTTATAGAAAAAATTACGTTTCAAGAAACAATAACATATGGGAGGAGAAGTTCTGATCTTAGTACCTCAATTGTACCTGAAATTTTGGTTTAGAAAATCACACTTGTCTTTTTTTGAAGATCCCAATGACTTGCAACTGAAATAGACCGCCTTCACAGGTTACAGGGAGGAGGGATCGAGCAAAACCTTTACCTTTTAAGTTGGATTCTAATGGTCCCTGGGGATTTTCTGGAGCAAATAAGGGGAACCCAAGGATCAGTGCTAAGTTCGTTGCTCCGTGTTACTATATCAACAAGTAAGAATTTCACTCTTATTCCCTTTGCGTTCACATTTGACTAATGCATAGCTTAAGATTGTTGGCATATTCCCTTTCACATTCTCTGATACTATATATCGATAATTATGCTTATGCAGGTATGATGATAGGAACCTCATGCATTAAGCAAAAGATTAGTAGGTAGCTTAGTAGAATGGTTGACAGCCTTTTGAGAGGGGACTATCTCTCTACCATATCTATCCCAACTTTCTCCTACATCTCCCTCTTCCTTTTAGACCTCTTACTATTAACAGCTTAACTAATTCACTTCCTCTTCCCAACTATATGCATAAAAAACTCCTAATTAACGACTAATCTGTTAGGAGGGGCATAAAAGGGGTGATATAGGAGGGAATCAATAGTTAGTAACAAGTGACTAGAGGAACTCTAGTGTGTGAGAGTACTAGACCTCTTGAAAGTCTGGAATTCTTGTAACTTTTCCACAATGAGAAATACAATGTTATTCAATTACTTTCGAGCTTTAATATTTTAACCTCCTCTATCGTGAGAGGGAAGAAGATGGAAAGCTGAAGGAATTGATGGAAACTTGATTAGGGGTGGTTGGTAACATGGGATGGTCTGAAAATTGTCAGTGGAGTTACTGTGCAACATCGGAAGGGAATAGGGATGAATCATCTGTTATGGAGGTAAGAAGAGACTGAGAAGACGATCAACCGTGGGTGCAAGTTGATAAGTCTGTTAGTAATAGGTGGATAGAAGGGGTGAGTCGGGTGACGACATAAGGATAAAGAAGGGAGCAAATAGTTGGTAAGAATTGACTGGAGAACTCCAGCGTGCAGAAATATCAAACCTCTCGTACGTTTAGAAGTTTTATAGCTTTTCCTTAAAAAGCAATACAATGGCAGTCCTTTCCTTTTAAGTGCTAACATAATATTAGCAAAAACTAAAATCAAATGAATTTGTTGGCAACTGTTTCTCCATGCTATTTTCTTTTGTTTAAATGCTCAACATGGTGAATTTTTTTATACACAAATCCTTATAGGTTCAACACTAACATAAACTTGTTTTCTCTTCAGAGTAGAGATAGACACTAAGCTTCCAGTACTTGGCGATCAAAAATGGGTAATATGGATTTGCTCCTTTAATGTGCCAATGGGTCCTGGAAAAACCCGTTCTATTGTATGCAGTGCTCGAAACTTCTTCCAGTTTTCTATGCCCGGTCCTGCTTGGTGGCAGGTAAGCAGTCCATGTTAACCAAATCTCTTCCCTGTTATTTATATATTAGAAGTATAAGTATTTTGATGAGATATTCCTCTTTCCATGTTTCACTTCCTACCTTTGCTTCTACTAGGTGGTCCCTAGATGGCACGAGCATTGGACGTCGAACAAGGTATATGATGGTGACATGATTGTGCTTCAGGGTCAGGAAAAGATATTTTTGTCGAAGGCCATGGAAGATTCGACAGATGTTAACAAAGAATACACTAAAATAACATTTACACCCACGCAAGCAGATCGGTTGGTGCTGGCATTCCGAAATTGGCTGAGGCGGCATGGCAAGGGCCAACCAGAATGGTTTGGGGCTAGCAGCCAGCAGCCTCTACCGTCAACCGTCCTATCTAAACGTCAGGTACAGAAAATTACCAGCATCTAGATTGTTGTAAATTTTAGATACAGTCCTATCTAACCTCTCTTTTCACCAAGTTTAGAACTGATAATTACTGCTTTTAGTTTTTTGCTAGTCTTAAAACTTATATATTATTATTAGTAGATGGTCCAATTTGAACCATAAGATTTTGGTTGTCCAATGACCAATAATACTACCCTTAAGATGTAACTCATGTTTGGTTGGTCGGTTTATGATTTCACCATGGAATCGTTGTGATAAGTACCAAACATAGACTCGACTTCTTCTTCTAGGTTACACAAATCGAGCTCGTGAGTAAAAATTCCCTACTTAGATTACGTTAGAATAATCTTATGAATCTAACTCATCTCTCACTTCACCCAATCTGCTGCCTGCGCAGATGTTGGACAGATTTGAGCAACACACACTCCATTGTTCCTCATGTAGAGGAGCTTATAAGTCATTTCAGACACTGCAGAAGCTGCTAATTGGTGCAACTGTAGTTTTTGCAGCAACGGCCGGTGTCCCGTCTACTCTGCAGATACGTATTCTTCTGGCGGGTCTCGCACTCGCAAGCGCTGGGATGGCTTATGCTTTGTTTGAACTCCAAAAGAATTTTGTGTTCATTGACTATGTTCATGCTGAGATTGATTAGCGAGATTCTACAATTCTAGGAATGTATAGTAAATTTTAGGAATGTATAGTAAAACGAAATTAGGGATAATTCTACACTTTCTTTTCTTGGTCCATGAGATTTCAGTAATGTTTCTAGATATTTAAATTTTAAAATTTCGTTACGAAGAAAATGATGGTACAAGTTTGTAAATTCATGAGTTTAATGTAGAAATAATGTGGCATTCTCAAAGTGACAAAAATGCT

mRNA sequence

ATGGCTCTTTCGATCGCTACCTCTGCTTCTTCTGCTACTCTTATTACCTCTTCGTTATATGATGTGAAATCTCGCAGAATAGTCCCCTTTGTAATCGCCGCGGATCGCAGCCCTAATTTTCTGGCGAGAAAATTGCCTAGTAGGGCAGCAGCTCTCAGGCCGGTTCGAGTAGCTGCGCCTCCAACGCCGCCCTCGACGGCAGATTCCGAGAAATCTGCGGTAGAGAATTCCGATGAAGTGGAAGTGGATGAGTCGGGCAATGAGAGCTCGTCTTCGCCGTTTTCTTGGAGAGATAATTGGTACCCTGTTTCCCTAATTGAAGATTTGGATCCTGCTTTGCCAACCCCGTTTCAGCTTCTGGGTAGAGATATCGTTCTTTGGTTTGATAAATCGAGAGGCGAGTGGGTCGCTTTTGATGATAAATGCCCTCACCGGCTTGCCCCTTTATCTGAAGGGCGGATAGATGAAGGTGGCAACTTGCAGTGCTCATACCATGGATGGTCCTTCGATGGGTGTGGATCTTGTGTTAAAATCCCTCAGGCATCATCAGAAGGCCCAGAATCTCGGGCTCATCAGTCGCCACGAGCATGTGCTACAAGGTTCCCTACTCTGGTCTCCCAAGGCCTTCTCTTTGTTTGGCCTGATGAGAACGGTTGGGAAAGAGTCAATGCCACAGCACCTCCAAGGCTCCCTGATGACTTCGATAAGCCTGAGTTCTCATCCGTTACGATTCAGCGGGATCTATTTTATGGTTATGATACACTAATGGAGAATGTCTCAGATCCTTCCCACATTGATTTTGCTCATCACAAGGTTACAGGGAGGAGGGATCGAGCAAAACCTTTACCTTTTAAGTTGGATTCTAATGGTCCCTGGGGATTTTCTGGAGCAAATAAGGGGAACCCAAGGATCAGTGCTAAGTTCGTTGCTCCGTGTTACTATATCAACAAAGTAGAGATAGACACTAAGCTTCCAGTACTTGGCGATCAAAAATGGGTAATATGGATTTGCTCCTTTAATGTGCCAATGGGTCCTGGAAAAACCCGTTCTATTGTATGCAGTGCTCGAAACTTCTTCCAGTTTTCTATGCCCGGTCCTGCTTGGTGGCAGGTGGTCCCTAGATGGCACGAGCATTGGACGTCGAACAAGGTATATGATGGTGACATGATTGTGCTTCAGGGTCAGGAAAAGATATTTTTGTCGAAGGCCATGGAAGATTCGACAGATGTTAACAAAGAATACACTAAAATAACATTTACACCCACGCAAGCAGATCGGTTGGTGCTGGCATTCCGAAATTGGCTGAGGCGGCATGGCAAGGGCCAACCAGAATGGTTTGGGGCTAGCAGCCAGCAGCCTCTACCGTCAACCGTCCTATCTAAACGTCAGATGTTGGACAGATTTGAGCAACACACACTCCATTGTTCCTCATGTAGAGGAGCTTATAAGTCATTTCAGACACTGCAGAAGCTGCTAATTGGTGCAACTGTAGTTTTTGCAGCAACGGCCGGTGTCCCGTCTACTCTGCAGATACGTATTCTTCTGGCGGGTCTCGCACTCGCAAGCGCTGGGATGGCTTATGCTTTGTTTGAACTCCAAAAGAATTTTGTGTTCATTGACTATGTTCATGCTGAGATTGATTAGCGAGATTCTACAATTCTAGGAATGTATAGTAAATTTTAGGAATGTATAGTAAAACGAAATTAGGGATAATTCTACACTTTCTTTTCTTGGTCCATGAGATTTCAGTAATGTTTCTAGATATTTAAATTTTAAAATTTCGTTACGAAGAAAATGATGGTACAAGTTTGTAAATTCATGAGTTTAATGTAGAAATAATGTGGCATTCTCAAAGTGACAAAAATGCT

Coding sequence (CDS)

ATGGCTCTTTCGATCGCTACCTCTGCTTCTTCTGCTACTCTTATTACCTCTTCGTTATATGATGTGAAATCTCGCAGAATAGTCCCCTTTGTAATCGCCGCGGATCGCAGCCCTAATTTTCTGGCGAGAAAATTGCCTAGTAGGGCAGCAGCTCTCAGGCCGGTTCGAGTAGCTGCGCCTCCAACGCCGCCCTCGACGGCAGATTCCGAGAAATCTGCGGTAGAGAATTCCGATGAAGTGGAAGTGGATGAGTCGGGCAATGAGAGCTCGTCTTCGCCGTTTTCTTGGAGAGATAATTGGTACCCTGTTTCCCTAATTGAAGATTTGGATCCTGCTTTGCCAACCCCGTTTCAGCTTCTGGGTAGAGATATCGTTCTTTGGTTTGATAAATCGAGAGGCGAGTGGGTCGCTTTTGATGATAAATGCCCTCACCGGCTTGCCCCTTTATCTGAAGGGCGGATAGATGAAGGTGGCAACTTGCAGTGCTCATACCATGGATGGTCCTTCGATGGGTGTGGATCTTGTGTTAAAATCCCTCAGGCATCATCAGAAGGCCCAGAATCTCGGGCTCATCAGTCGCCACGAGCATGTGCTACAAGGTTCCCTACTCTGGTCTCCCAAGGCCTTCTCTTTGTTTGGCCTGATGAGAACGGTTGGGAAAGAGTCAATGCCACAGCACCTCCAAGGCTCCCTGATGACTTCGATAAGCCTGAGTTCTCATCCGTTACGATTCAGCGGGATCTATTTTATGGTTATGATACACTAATGGAGAATGTCTCAGATCCTTCCCACATTGATTTTGCTCATCACAAGGTTACAGGGAGGAGGGATCGAGCAAAACCTTTACCTTTTAAGTTGGATTCTAATGGTCCCTGGGGATTTTCTGGAGCAAATAAGGGGAACCCAAGGATCAGTGCTAAGTTCGTTGCTCCGTGTTACTATATCAACAAAGTAGAGATAGACACTAAGCTTCCAGTACTTGGCGATCAAAAATGGGTAATATGGATTTGCTCCTTTAATGTGCCAATGGGTCCTGGAAAAACCCGTTCTATTGTATGCAGTGCTCGAAACTTCTTCCAGTTTTCTATGCCCGGTCCTGCTTGGTGGCAGGTGGTCCCTAGATGGCACGAGCATTGGACGTCGAACAAGGTATATGATGGTGACATGATTGTGCTTCAGGGTCAGGAAAAGATATTTTTGTCGAAGGCCATGGAAGATTCGACAGATGTTAACAAAGAATACACTAAAATAACATTTACACCCACGCAAGCAGATCGGTTGGTGCTGGCATTCCGAAATTGGCTGAGGCGGCATGGCAAGGGCCAACCAGAATGGTTTGGGGCTAGCAGCCAGCAGCCTCTACCGTCAACCGTCCTATCTAAACGTCAGATGTTGGACAGATTTGAGCAACACACACTCCATTGTTCCTCATGTAGAGGAGCTTATAAGTCATTTCAGACACTGCAGAAGCTGCTAATTGGTGCAACTGTAGTTTTTGCAGCAACGGCCGGTGTCCCGTCTACTCTGCAGATACGTATTCTTCTGGCGGGTCTCGCACTCGCAAGCGCTGGGATGGCTTATGCTTTGTTTGAACTCCAAAAGAATTTTGTGTTCATTGACTATGTTCATGCTGAGATTGATTAG

Protein sequence

MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAPPTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKGNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFTPTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRGAYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFIDYVHAEID
Homology
BLAST of MC03g0378 vs. ExPASy Swiss-Prot
Match: Q9FYC2 (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO PE=1 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 6.4e-235
Identity = 386/548 (70.44%), Postives = 462/548 (84.31%), Query Frame = 0

Query: 3   LSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAAL-RPVRVAAPP 62
           +S+   +S++  IT S    +S++I PF+    + P  ++   PSR+     P+RVAAPP
Sbjct: 1   MSVVLLSSTSATITKS----QSKKI-PFLSPTTKFPLKVSIS-PSRSKLFHNPLRVAAPP 60

Query: 63  TPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLG 122
           + P++  +E+  +E     + +E G+E     F WRD+WYPVSL+EDLDP +PTPFQLLG
Sbjct: 61  SVPTSDSTEEKRIEEEYGGDKEEEGSE-----FKWRDHWYPVSLVEDLDPNVPTPFQLLG 120

Query: 123 RDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQA 182
           RD+VLWFD++  +W AFDD CPHRLAPLSEGR+DE G+LQCSYHGWSF GCGSC +IPQA
Sbjct: 121 RDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQA 180

Query: 183 SSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFSS 242
           ++ GPE+RA +SPRACA +FPT+VSQGLLFVWPDENGW+R N+  PPRLPDDFDKPEFS+
Sbjct: 181 ATSGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFST 240

Query: 243 VTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKGN 302
           VTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFK++S+GPWGF GAN  +
Sbjct: 241 VTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQGANDDS 300

Query: 303 PRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQF 362
           PRI+AKFVAPCY +NK+E+D KLP++G+QKWVIWICSFN+PM PGKTRSIVCSARNFFQF
Sbjct: 301 PRITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQF 360

Query: 363 SMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMED-STDVNKEYTKITFT 422
           S+PGPAWWQVVPRW+EHWTSN VYDGDMIVLQGQEK+FL+K+ME    DVNK+YTK+TFT
Sbjct: 361 SVPGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFT 420

Query: 423 PTQADRLVLAFRNWLRRHGKGQPEWFGAS-SQQPLPSTVLSKRQMLDRFEQHTLHCSSCR 482
           PTQADR VLAFRNWLRRHGK QPEWFG++ S QPLPSTVL+KRQMLDRF+QHT  CSSC+
Sbjct: 421 PTQADRFVLAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCK 480

Query: 483 GAYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFI 542
           GAY SFQ L+K L+GATV +AATAGVPS +QIR++LAGL+L SA  AYAL E +KNFVF 
Sbjct: 481 GAYNSFQILKKFLVGATVFWAATAGVPSDVQIRLVLAGLSLISAASAYALHEQEKNFVFR 537

Query: 543 DYVHAEID 548
           DYVH+EI+
Sbjct: 541 DYVHSEIE 537

BLAST of MC03g0378 vs. ExPASy Swiss-Prot
Match: Q0DV66 (Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PAO PE=2 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 3.7e-227
Identity = 374/539 (69.39%), Postives = 434/539 (80.52%), Query Frame = 0

Query: 9   ASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAPPTPPSTAD 68
           A +A+L+ S      SRR VP + A   S     R    RA A   +RVAAPP+ P  AD
Sbjct: 5   APTASLLLSPRPLPASRR-VPSLPALSASGRLRLR----RARADTRLRVAAPPSVPGEAD 64

Query: 69  SEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLGRDIVLWF 128
                 E S           S+   F WRD+WYPVSL+EDLDP++PTPFQLL RD+V+W 
Sbjct: 65  QAPGETEPS---------TSSADEKFVWRDHWYPVSLVEDLDPSVPTPFQLLNRDLVIWK 124

Query: 129 DKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASSEGPES 188
           D   GEWVA DD+CPHRLAPLSEGRIDE G LQCSYHGWSFDG G+C +IPQA+ EGPE+
Sbjct: 125 DPKSGEWVALDDRCPHRLAPLSEGRIDETGCLQCSYHGWSFDGSGACTRIPQAAPEGPEA 184

Query: 189 RAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFSSVTIQRDL 248
           +A +SP+ACA +FPTLVSQGLLFVWPDENGWE+  AT PP LP +F+ P FS+VTIQRDL
Sbjct: 185 KAVRSPKACAIKFPTLVSQGLLFVWPDENGWEKATATKPPMLPKEFEDPAFSTVTIQRDL 244

Query: 249 FYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKGNPRISAKF 308
           +YGYDTLMENVSDPSHI+FAHHKVTGRRDRA+PLPFK++S+G WG+SG+N GNPRISA F
Sbjct: 245 YYGYDTLMENVSDPSHIEFAHHKVTGRRDRARPLPFKMESSGAWGYSGSNSGNPRISATF 304

Query: 309 VAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPGPAW 368
           VAPCY +NK+EIDTKLP+ GDQKWVIWICSFN+PM PGKTRSIVCSARNFFQFSMPG AW
Sbjct: 305 VAPCYALNKIEIDTKLPIFGDQKWVIWICSFNIPMAPGKTRSIVCSARNFFQFSMPGKAW 364

Query: 369 WQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFTPTQADRLV 428
           WQ+VPRW+EHWTSN VYDGDMIVLQGQEKIFLS + E S D+N++YTKITFTPTQADR V
Sbjct: 365 WQLVPRWYEHWTSNLVYDGDMIVLQGQEKIFLSASKESSADINQQYTKITFTPTQADRFV 424

Query: 429 LAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRGAYKSFQTL 488
           LAFR WLR+ G  QP+WFG  SQ+ LPSTVLSKR+MLDR+EQHTL CSSC+GAY +FQTL
Sbjct: 425 LAFRAWLRKFGNSQPDWFGNPSQEVLPSTVLSKREMLDRYEQHTLKCSSCKGAYNAFQTL 484

Query: 489 QKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFIDYVHAEID 548
           QK+ +GATV F ATAG+P+ +Q R+LLA  AL SA +AYA + LQKNFVF+DYVHAEID
Sbjct: 485 QKVFMGATVAFCATAGIPADVQFRLLLAAAALVSAAVAYAFYTLQKNFVFVDYVHAEID 529

BLAST of MC03g0378 vs. ExPASy Swiss-Prot
Match: Q8W496 (Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTC52 PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 7.1e-69
Identity = 178/571 (31.17%), Postives = 273/571 (47.81%), Query Frame = 0

Query: 37  SPNFLARKLPSRAAALRPVRVAAPPTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSW 96
           SPN L  +  SR          A  +PPS+     SA  +++     E+  E  S  F W
Sbjct: 34  SPNSLITRKSSRF-------TTAVSSPPSS-----SAATSTNSPPEPEALFEPGSDKFDW 93

Query: 97  RDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDE 156
             NWYPV  I DLD  +P   +++G D+V+W+D++  +W   DD CPHRLAPLS+GRID+
Sbjct: 94  YANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQ 153

Query: 157 GGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDE 216
            G LQC YHGW F+G G C  IPQA  +GP    H   +AC   +P+ V   +++ WP+ 
Sbjct: 154 WGRLQCVYHGWCFNGSGDCKLIPQAPPDGPP--VHTFKQACVAVYPSTVQHEIIWFWPNS 213

Query: 217 NGWER--VNATAPPRLPDDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHH---- 276
           +   +  +    PP +P + + P F+ +   RD+ YGYD L+EN+ DP+H+ +AH+    
Sbjct: 214 DPKYKNIIETNKPPYIP-ELEDPSFTKLMGNRDIPYGYDVLVENLMDPAHVPYAHYGLMR 273

Query: 277 --KVTGR-------------------------RDRAKPLPF---KLDSNG-----PWGFS 336
             K  G+                         R+  KPL     KLD+ G      WG+S
Sbjct: 274 FPKPKGKYIICISNSCFNPFTNLQILLAEKIDREGGKPLEINVKKLDNKGFFSKQEWGYS 333

Query: 337 GANKGNPRISAKFVAPCYY------INKVEIDTKLPVLGDQK-------WVIWICSFNVP 396
                       F+APC Y      + + E +   P   D+         +I+IC   +P
Sbjct: 334 -----------NFIAPCVYRSSTDPLPEQEHEYPAPAASDKAALSKRRLSLIFIC---IP 393

Query: 397 MGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSK 456
           + PG++R I    RNF  F        ++VPRW  H   N + D D+ +L  +E+  L +
Sbjct: 394 VSPGRSRLIWTFPRNFGVFID------KIVPRWVFHIGQNTILDSDLHLLHVEERKILER 453

Query: 457 AMEDSTDVNKEYTKITFTPTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKR 516
             E+       + K  F PT++D  V+ FR W  ++ + + +W G      LP T   + 
Sbjct: 454 GPEN-------WQKACFIPTKSDANVVTFRRWFNKYSEARVDWRGKFDPFLLPPTP-PRE 513

Query: 517 QMLDRFEQHTLHCSSCRGAYKSFQTLQKLL-------IGATVVFAATAGVPSTLQIRILL 547
           Q+ DR+  H  +CSSC+ A+K    L+ +L       IG   V   T  + +  +I +L+
Sbjct: 514 QLFDRYWSHVENCSSCKKAHKYLNALEVILQIASVAMIGVMAVLKQTT-MSNVARIAVLV 559

BLAST of MC03g0378 vs. ExPASy Swiss-Prot
Match: Q9SK50 (Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.5e-45
Identity = 140/511 (27.40%), Postives = 224/511 (43.84%), Query Frame = 0

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSP----------FSWRDNWYPVSLIEDLD 120
           PT P      +SAV  +   +  E G +   +P          + W + WYP+ L +++ 
Sbjct: 39  PTKPRLRLLRRSAVAGTAVSDQTEGGGDVLLNPEEEKRVEVADYDWTEEWYPLYLTKNVP 98

Query: 121 PALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFD 180
              P    +  R IVL+ D   G    ++D+CPHRLA LSEG++ + G L+C YHGW F+
Sbjct: 99  EDAPLGLTVYDRQIVLYKD-GEGTLRCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFE 158

Query: 181 GCGSCVKIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRL 240
           G G CVKIPQ  +     +A     AC   +    SQG+++VW             P +L
Sbjct: 159 GEGKCVKIPQLPASAKIPKA-----ACVKTYEVKDSQGVVWVWMSTK-----TPPNPEKL 218

Query: 241 P--DDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKL 300
           P  ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   T +R+ A+PL F++
Sbjct: 219 PWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEV 278

Query: 301 DSNGPWGFSGA-----NKGNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNV 360
                 GF+G        G      +F APC   N  E + K  V       +++C    
Sbjct: 279 TERSNRGFAGTWGREKEGGKGSNLLRFDAPCVLQNNREFEGKDGVKNYFSG-LFLCR--- 338

Query: 361 PMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS 420
           P G GK+  IV      F  +   P    V+P+W  H  + KV++ DM  L  Q ++   
Sbjct: 339 PTGQGKSMLIV-----RFGVTKRSPL-VSVLPQWFWHQNACKVFEQDMGFLSSQNEVL-- 398

Query: 421 KAMEDSTDVNKEYTKITFTPTQADRLVLAFRNWLRRHGKGQPEWFG-------------- 480
             M++       Y  +      +D  V  +R W+ + G G P  FG              
Sbjct: 399 --MKEKVPTKDLYLNL----KSSDTWVAEYRKWMDKVGHGMPYHFGHRTISLPKVPPVVE 458

Query: 481 ---------ASSQQPLPSTV--LSKRQMLDRFEQHTLHCSSCRGAYKSFQTLQKLLIGAT 528
                     S+  P    +  +    + +R+ +H +HC SC    KSF+  + +L    
Sbjct: 459 HAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSCSNVIKSFELWKNILSATA 518

BLAST of MC03g0378 vs. ExPASy Swiss-Prot
Match: O49931 (Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.6e-44
Identity = 155/566 (27.39%), Postives = 257/566 (45.41%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLY--DVKSRRIVPFVIAADRSPNFLARKLPS--RAAALRPVR 60
           MAL++A SA+S  L T + +   V        ++  + S NF   K  S  R      V 
Sbjct: 1   MALALA-SANSFLLPTKTHFALHVSPPPSKKTLLCTNPSSNFSFNKALSSRRRKQAWCVA 60

Query: 61  VAAPPTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTP 120
            AA     +  D E+            E   E   + + W + WYP+ L +++    P  
Sbjct: 61  AAADVKDATLLDGEEDQKVLVGPSSEQERKGEREVADYDWTEEWYPLYLTKNVPHDAPLG 120

Query: 121 FQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCV 180
            ++  ++IVL+ D    ++  ++D+CPHRLA LSEG++ + G L+C YHGW F+G G CV
Sbjct: 121 LKVYDKNIVLFRD-GNDQFQCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFEGEGKCV 180

Query: 181 KIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPP---RLP-- 240
           KIPQ  ++    ++     AC   +    SQG+L+VW        ++   PP   ++P  
Sbjct: 181 KIPQLPADAKIPKS-----ACVKTYEVRDSQGVLWVW--------MSRKTPPNVSKIPWF 240

Query: 241 DDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKLDSN 300
           ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   + +R+ A+ L F++   
Sbjct: 241 ENFARPGFQDISTTHELPYDHSILLENLMDPAHVPISHDRTDWSAKREDAQALGFEVTER 300

Query: 301 GPWGFSG----ANKGNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGP 360
              GF+G       G+     +F APC   N  EI  K   +      +++C    P G 
Sbjct: 301 TDRGFAGWWGREKDGSKPNFLRFEAPCVLQNNREIVDKNGEINHFSG-LFLCR---PTGQ 360

Query: 361 GKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAME 420
           GK+  IV      F  +   P   ++ P W+ H  ++KV++ DM  L  Q +I L + + 
Sbjct: 361 GKSMLIV-----RFGATKRSPL-IKLFPEWYFHQNASKVFEQDMGFLSSQNEILLKEKVP 420

Query: 421 DSTDVNKEYTKITFTPTQADRLVLAFRNWLRRHGKGQPEWFGAS--SQQPLPSTV----- 480
                     ++      +D  V  +R W+ + G G P  FG S  S    P+ V     
Sbjct: 421 TK--------ELYLNLKSSDTWVAEYRKWMDKVGHGMPYHFGHSTISLPEEPAVVEHAPA 480

Query: 481 ------------------LSKRQMLDRFEQHTLHCSSCRGAYKSFQTLQKLLIGATVVFA 526
                             +    + +R+ +H +HC  C  A K+FQ  + +L G  V  A
Sbjct: 481 GLVAGLSASSPAKGGIGTMHAPNLANRYFRHVIHCKGCSSAIKAFQIWKNVLSGVVVALA 532

BLAST of MC03g0378 vs. NCBI nr
Match: XP_022152935.1 (pheophorbide a oxygenase, chloroplastic [Momordica charantia])

HSP 1 Score: 1115 bits (2885), Expect = 0.0
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP
Sbjct: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID
Sbjct: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 547

BLAST of MC03g0378 vs. NCBI nr
Match: XP_022964963.1 (pheophorbide a oxygenase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1046 bits (2705), Expect = 0.0
Identity = 512/547 (93.60%), Postives = 524/547 (95.80%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSIATSA SATLITSSL  VKS RIVP +I +DR+PNFL+RK   RA+ +RPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSLIVSDRNPNFLSRKSHIRASTIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKS  EN DEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSEAENLDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPT+VSQGLLFVWPDENGWER NAT PPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  MEDSTDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEDSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQTLQKLLIGATVVFAATAG+PS LQ+RILLA LAL SAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTLQKLLIGATVVFAATAGIPSALQMRILLAALALTSAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. NCBI nr
Match: XP_008462467.1 (PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo] >KAA0032593.1 pheophorbide a oxygenase [Cucumis melo var. makuwa] >TYK20892.1 pheophorbide a oxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 504/547 (92.14%), Postives = 526/547 (96.16%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSI+TS  S+TLITSSLYDVKS RIVP +IA DR+PNF  RKL S A+ +RP+RVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRNPNFWLRKLRSTASCIRPLRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS V +SD+VEVDESGNESSS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEVVDSDDVEVDESGNESSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKS GEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSSGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWER NAT PPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKF+APCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  ME STDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEGSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWF ASSQQPLPSTVLSKRQMLDRFEQHTLHCSSC+G
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFRASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQT+Q+LLIGATVVF+ATAG+PS+LQIR+LLA LALASAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTVQRLLIGATVVFSATAGIPSSLQIRVLLASLALASAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. NCBI nr
Match: XP_022970307.1 (pheophorbide a oxygenase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 510/547 (93.24%), Postives = 522/547 (95.43%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSIATSA SATLITSSL  VKS RIVP VI +DR+PNFL+RK   RA+A+RPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSVIVSDRNPNFLSRKSHIRASAIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS  EN DEVEV E GNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEAENLDEVEVVELGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPT+VSQGLLFVWPDENGWER NAT PPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPV+GDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVVGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  MEDSTDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEDSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQTLQKLLIGATVVFAATAG+PS LQ+RILLA LAL SAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTLQKLLIGATVVFAATAGIPSALQMRILLAALALTSAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. NCBI nr
Match: XP_023519435.1 (pheophorbide a oxygenase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1040 bits (2689), Expect = 0.0
Identity = 511/548 (93.25%), Postives = 526/548 (95.99%), Query Frame = 0

Query: 1   MALSIATSAS-SATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAA 60
           MALSIATSA+ SATLITSSL  V+S RIVP +I +DR+PNFL+RK   RA+A+RPVRVAA
Sbjct: 1   MALSIATSAAVSATLITSSLNHVESLRIVPSLIVSDRNPNFLSRKSHIRASAIRPVRVAA 60

Query: 61  PPTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQL 120
           PPTPPSTADSEKS  EN DEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQL
Sbjct: 61  PPTPPSTADSEKSEAENLDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQL 120

Query: 121 LGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIP 180
           LGRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIP
Sbjct: 121 LGRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIP 180

Query: 181 QASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEF 240
           QAS+EGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWER NAT PPRLPDDF KPEF
Sbjct: 181 QASAEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEF 240

Query: 241 SSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANK 300
           SSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANK
Sbjct: 241 SSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANK 300

Query: 301 GNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFF 360
           GNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFF
Sbjct: 301 GNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFF 360

Query: 361 QFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITF 420
           QFSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  ME STDVNKEYTKITF
Sbjct: 361 QFSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEGSTDVNKEYTKITF 420

Query: 421 TPTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCR 480
           TPTQADRLVLAFRNWLRRHGKGQPEWFGAS+QQPLPSTVLSKRQMLDRFEQHTLHCSSCR
Sbjct: 421 TPTQADRLVLAFRNWLRRHGKGQPEWFGASNQQPLPSTVLSKRQMLDRFEQHTLHCSSCR 480

Query: 481 GAYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFI 540
           GAYK+FQTLQKLLIGATVVFAATAG+PS LQ+RILLA LALASAG+AYALFELQKNFVFI
Sbjct: 481 GAYKTFQTLQKLLIGATVVFAATAGIPSALQMRILLAALALASAGLAYALFELQKNFVFI 540

Query: 541 DYVHAEID 547
           DYVHAEID
Sbjct: 541 DYVHAEID 546

BLAST of MC03g0378 vs. ExPASy TrEMBL
Match: A0A6J1DFC6 (pheophorbide a oxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111020548 PE=4 SV=1)

HSP 1 Score: 1115 bits (2885), Expect = 0.0
Identity = 547/547 (100.00%), Postives = 547/547 (100.00%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP
Sbjct: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID
Sbjct: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 547

BLAST of MC03g0378 vs. ExPASy TrEMBL
Match: A0A6J1HMG2 (pheophorbide a oxygenase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464914 PE=4 SV=1)

HSP 1 Score: 1046 bits (2705), Expect = 0.0
Identity = 512/547 (93.60%), Postives = 524/547 (95.80%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSIATSA SATLITSSL  VKS RIVP +I +DR+PNFL+RK   RA+ +RPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSLIVSDRNPNFLSRKSHIRASTIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPSTADSEKS  EN DEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTADSEKSEAENLDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPT+VSQGLLFVWPDENGWER NAT PPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  MEDSTDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEDSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQTLQKLLIGATVVFAATAG+PS LQ+RILLA LAL SAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTLQKLLIGATVVFAATAGIPSALQMRILLAALALTSAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. ExPASy TrEMBL
Match: A0A5D3DBC9 (Pheophorbide a oxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold284G00270 PE=4 SV=1)

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 504/547 (92.14%), Postives = 526/547 (96.16%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSI+TS  S+TLITSSLYDVKS RIVP +IA DR+PNF  RKL S A+ +RP+RVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRNPNFWLRKLRSTASCIRPLRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS V +SD+VEVDESGNESSS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEVVDSDDVEVDESGNESSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKS GEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSSGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWER NAT PPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKF+APCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  ME STDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEGSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWF ASSQQPLPSTVLSKRQMLDRFEQHTLHCSSC+G
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFRASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQT+Q+LLIGATVVF+ATAG+PS+LQIR+LLA LALASAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTVQRLLIGATVVFSATAGIPSSLQIRVLLASLALASAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. ExPASy TrEMBL
Match: A0A6J1HYR3 (pheophorbide a oxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469310 PE=4 SV=1)

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 510/547 (93.24%), Postives = 522/547 (95.43%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSIATSA SATLITSSL  VKS RIVP VI +DR+PNFL+RK   RA+A+RPVRVAAP
Sbjct: 1   MALSIATSAVSATLITSSLNHVKSLRIVPSVIVSDRNPNFLSRKSHIRASAIRPVRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS  EN DEVEV E GNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEAENLDEVEVVELGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKSR EWVAFDDKCPHRLAPLSEGRIDE GNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSRSEWVAFDDKCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPT+VSQGLLFVWPDENGWER NAT PPRLPDDF KPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTMVSQGLLFVWPDENGWERANATTPPRLPDDFYKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKFVAPCYYINKVEIDTKLPV+GDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFVAPCYYINKVEIDTKLPVVGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQ+VPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  MEDSTDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQLVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEDSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQTLQKLLIGATVVFAATAG+PS LQ+RILLA LAL SAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTLQKLLIGATVVFAATAGIPSALQMRILLAALALTSAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. ExPASy TrEMBL
Match: A0A1S3CII7 (pheophorbide a oxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500813 PE=4 SV=1)

HSP 1 Score: 1040 bits (2690), Expect = 0.0
Identity = 504/547 (92.14%), Postives = 526/547 (96.16%), Query Frame = 0

Query: 1   MALSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAALRPVRVAAP 60
           MALSI+TS  S+TLITSSLYDVKS RIVP +IA DR+PNF  RKL S A+ +RP+RVAAP
Sbjct: 1   MALSISTSLFSSTLITSSLYDVKSLRIVPSLIAVDRNPNFWLRKLRSTASCIRPLRVAAP 60

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLL 120
           PTPPST DSEKS V +SD+VEVDESGNESSS+PFSWRDNWYPVSLIEDLDPALPTPFQLL
Sbjct: 61  PTPPSTVDSEKSEVVDSDDVEVDESGNESSSTPFSWRDNWYPVSLIEDLDPALPTPFQLL 120

Query: 121 GRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180
           GRDIVLWFDKS GEWVAFDD+CPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ
Sbjct: 121 GRDIVLWFDKSSGEWVAFDDRCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQ 180

Query: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFS 240
           ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWER NAT PPRLPDDFDKPEFS
Sbjct: 181 ASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERANATTPPRLPDDFDKPEFS 240

Query: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300
           SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG
Sbjct: 241 SVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKG 300

Query: 301 NPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360
           NPRISAKF+APCYYINKVEIDTKLP+LGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ
Sbjct: 301 NPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQ 360

Query: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFT 420
           FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS  ME STDVNKEYTKITFT
Sbjct: 361 FSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS--MEGSTDVNKEYTKITFT 420

Query: 421 PTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRG 480
           PTQADRLVLAFRNWLRRHGKGQPEWF ASSQQPLPSTVLSKRQMLDRFEQHTLHCSSC+G
Sbjct: 421 PTQADRLVLAFRNWLRRHGKGQPEWFRASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCKG 480

Query: 481 AYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFID 540
           AYK+FQT+Q+LLIGATVVF+ATAG+PS+LQIR+LLA LALASAG+AYALFELQKNFVFID
Sbjct: 481 AYKTFQTVQRLLIGATVVFSATAGIPSSLQIRVLLASLALASAGLAYALFELQKNFVFID 540

Query: 541 YVHAEID 547
           YVHAEID
Sbjct: 541 YVHAEID 545

BLAST of MC03g0378 vs. TAIR 10
Match: AT3G44880.1 (Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain )

HSP 1 Score: 814.7 bits (2103), Expect = 4.5e-236
Identity = 386/548 (70.44%), Postives = 462/548 (84.31%), Query Frame = 0

Query: 3   LSIATSASSATLITSSLYDVKSRRIVPFVIAADRSPNFLARKLPSRAAAL-RPVRVAAPP 62
           +S+   +S++  IT S    +S++I PF+    + P  ++   PSR+     P+RVAAPP
Sbjct: 1   MSVVLLSSTSATITKS----QSKKI-PFLSPTTKFPLKVSIS-PSRSKLFHNPLRVAAPP 60

Query: 63  TPPSTADSEKSAVENSDEVEVDESGNESSSSPFSWRDNWYPVSLIEDLDPALPTPFQLLG 122
           + P++  +E+  +E     + +E G+E     F WRD+WYPVSL+EDLDP +PTPFQLLG
Sbjct: 61  SVPTSDSTEEKRIEEEYGGDKEEEGSE-----FKWRDHWYPVSLVEDLDPNVPTPFQLLG 120

Query: 123 RDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQA 182
           RD+VLWFD++  +W AFDD CPHRLAPLSEGR+DE G+LQCSYHGWSF GCGSC +IPQA
Sbjct: 121 RDLVLWFDRNDQKWAAFDDLCPHRLAPLSEGRLDENGHLQCSYHGWSFGGCGSCTRIPQA 180

Query: 183 SSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRLPDDFDKPEFSS 242
           ++ GPE+RA +SPRACA +FPT+VSQGLLFVWPDENGW+R N+  PPRLPDDFDKPEFS+
Sbjct: 181 ATSGPEARAVKSPRACAIKFPTMVSQGLLFVWPDENGWDRANSIEPPRLPDDFDKPEFST 240

Query: 243 VTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSGANKGN 302
           VTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFK++S+GPWGF GAN  +
Sbjct: 241 VTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQGANDDS 300

Query: 303 PRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNVPMGPGKTRSIVCSARNFFQF 362
           PRI+AKFVAPCY +NK+E+D KLP++G+QKWVIWICSFN+PM PGKTRSIVCSARNFFQF
Sbjct: 301 PRITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSARNFFQF 360

Query: 363 SMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMED-STDVNKEYTKITFT 422
           S+PGPAWWQVVPRW+EHWTSN VYDGDMIVLQGQEK+FL+K+ME    DVNK+YTK+TFT
Sbjct: 361 SVPGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYTKLTFT 420

Query: 423 PTQADRLVLAFRNWLRRHGKGQPEWFGAS-SQQPLPSTVLSKRQMLDRFEQHTLHCSSCR 482
           PTQADR VLAFRNWLRRHGK QPEWFG++ S QPLPSTVL+KRQMLDRF+QHT  CSSC+
Sbjct: 421 PTQADRFVLAFRNWLRRHGKSQPEWFGSTPSNQPLPSTVLTKRQMLDRFDQHTQVCSSCK 480

Query: 483 GAYKSFQTLQKLLIGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVFI 542
           GAY SFQ L+K L+GATV +AATAGVPS +QIR++LAGL+L SA  AYAL E +KNFVF 
Sbjct: 481 GAYNSFQILKKFLVGATVFWAATAGVPSDVQIRLVLAGLSLISAASAYALHEQEKNFVFR 537

Query: 543 DYVHAEID 548
           DYVH+EI+
Sbjct: 541 DYVHSEIE 537

BLAST of MC03g0378 vs. TAIR 10
Match: AT4G25650.1 (ACD1-like )

HSP 1 Score: 270.8 bits (691), Expect = 2.4e-72
Identity = 177/548 (32.30%), Postives = 271/548 (49.45%), Query Frame = 0

Query: 37  SPNFLARKLPSRAAALRPVRVAAPPTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSW 96
           SPN L  +  SR          A  +PPS+     SA  +++     E+  E  S  F W
Sbjct: 34  SPNSLITRKSSRF-------TTAVSSPPSS-----SAATSTNSPPEPEALFEPGSDKFDW 93

Query: 97  RDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDE 156
             NWYPV  I DLD  +P   +++G D+V+W+D++  +W   DD CPHRLAPLS+GRID+
Sbjct: 94  YANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQ 153

Query: 157 GGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDE 216
            G LQC YHGW F+G G C  IPQA  +GP    H   +AC   +P+ V   +++ WP+ 
Sbjct: 154 WGRLQCVYHGWCFNGSGDCKLIPQAPPDGPP--VHTFKQACVAVYPSTVQHEIIWFWPNS 213

Query: 217 NGWER--VNATAPPRLPDDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHH---- 276
           +   +  +    PP +P + + P F+ +   RD+ YGYD L+EN+ DP+H+ +AH+    
Sbjct: 214 DPKYKNIIETNKPPYIP-ELEDPSFTKLMGNRDIPYGYDVLVENLMDPAHVPYAHYGLMR 273

Query: 277 ----KVTGRRDRAKPLPF---KLDSNG-----PWGFSGANKGNPRISAKFVAPCYY---- 336
               K    R+  KPL     KLD+ G      WG+S            F+APC Y    
Sbjct: 274 FPKPKEKIDREGGKPLEINVKKLDNKGFFSKQEWGYS-----------NFIAPCVYRSST 333

Query: 337 --INKVEIDTKLPVLGDQK-------WVIWICSFNVPMGPGKTRSIVCSARNFFQFSMPG 396
             + + E +   P   D+         +I+IC   +P+ PG++R I    RNF  F    
Sbjct: 334 DPLPEQEHEYPAPAASDKAALSKRRLSLIFIC---IPVSPGRSRLIWTFPRNFGVFID-- 393

Query: 397 PAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKAMEDSTDVNKEYTKITFTPTQAD 456
               ++VPRW  H   N + D D+ +L  +E+  L +  E+       + K  F PT++D
Sbjct: 394 ----KIVPRWVFHIGQNTILDSDLHLLHVEERKILERGPEN-------WQKACFIPTKSD 453

Query: 457 RLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKRQMLDRFEQHTLHCSSCRGAYKSF 516
             V+ FR W  ++ + + +W G      LP T   + Q+ DR+  H  +CSSC+ A+K  
Sbjct: 454 ANVVTFRRWFNKYSEARVDWRGKFDPFLLPPTP-PREQLFDRYWSHVENCSSCKKAHKYL 513

Query: 517 QTLQKLL-------IGATVVFAATAGVPSTLQIRILLAGLALASAGMAYALFELQKNFVF 547
             L+ +L       IG   V   T  + +  +I +L+A + L+ A   +    + K F +
Sbjct: 514 NALEVILQIASVAMIGVMAVLKQTT-MSNVARIAVLVAAV-LSFAASKWLSHFIYKTFHY 536

BLAST of MC03g0378 vs. TAIR 10
Match: AT4G25650.2 (ACD1-like )

HSP 1 Score: 263.1 bits (671), Expect = 5.1e-70
Identity = 178/571 (31.17%), Postives = 273/571 (47.81%), Query Frame = 0

Query: 37  SPNFLARKLPSRAAALRPVRVAAPPTPPSTADSEKSAVENSDEVEVDESGNESSSSPFSW 96
           SPN L  +  SR          A  +PPS+     SA  +++     E+  E  S  F W
Sbjct: 34  SPNSLITRKSSRF-------TTAVSSPPSS-----SAATSTNSPPEPEALFEPGSDKFDW 93

Query: 97  RDNWYPVSLIEDLDPALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDE 156
             NWYPV  I DLD  +P   +++G D+V+W+D++  +W   DD CPHRLAPLS+GRID+
Sbjct: 94  YANWYPVMPICDLDKKVPHGKKVMGIDLVVWWDRNEKQWKVMDDTCPHRLAPLSDGRIDQ 153

Query: 157 GGNLQCSYHGWSFDGCGSCVKIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDE 216
            G LQC YHGW F+G G C  IPQA  +GP    H   +AC   +P+ V   +++ WP+ 
Sbjct: 154 WGRLQCVYHGWCFNGSGDCKLIPQAPPDGPP--VHTFKQACVAVYPSTVQHEIIWFWPNS 213

Query: 217 NGWER--VNATAPPRLPDDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHH---- 276
           +   +  +    PP +P + + P F+ +   RD+ YGYD L+EN+ DP+H+ +AH+    
Sbjct: 214 DPKYKNIIETNKPPYIP-ELEDPSFTKLMGNRDIPYGYDVLVENLMDPAHVPYAHYGLMR 273

Query: 277 --KVTGR-------------------------RDRAKPLPF---KLDSNG-----PWGFS 336
             K  G+                         R+  KPL     KLD+ G      WG+S
Sbjct: 274 FPKPKGKYIICISNSCFNPFTNLQILLAEKIDREGGKPLEINVKKLDNKGFFSKQEWGYS 333

Query: 337 GANKGNPRISAKFVAPCYY------INKVEIDTKLPVLGDQK-------WVIWICSFNVP 396
                       F+APC Y      + + E +   P   D+         +I+IC   +P
Sbjct: 334 -----------NFIAPCVYRSSTDPLPEQEHEYPAPAASDKAALSKRRLSLIFIC---IP 393

Query: 397 MGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSK 456
           + PG++R I    RNF  F        ++VPRW  H   N + D D+ +L  +E+  L +
Sbjct: 394 VSPGRSRLIWTFPRNFGVFID------KIVPRWVFHIGQNTILDSDLHLLHVEERKILER 453

Query: 457 AMEDSTDVNKEYTKITFTPTQADRLVLAFRNWLRRHGKGQPEWFGASSQQPLPSTVLSKR 516
             E+       + K  F PT++D  V+ FR W  ++ + + +W G      LP T   + 
Sbjct: 454 GPEN-------WQKACFIPTKSDANVVTFRRWFNKYSEARVDWRGKFDPFLLPPTP-PRE 513

Query: 517 QMLDRFEQHTLHCSSCRGAYKSFQTLQKLL-------IGATVVFAATAGVPSTLQIRILL 547
           Q+ DR+  H  +CSSC+ A+K    L+ +L       IG   V   T  + +  +I +L+
Sbjct: 514 QLFDRYWSHVENCSSCKKAHKYLNALEVILQIASVAMIGVMAVLKQTT-MSNVARIAVLV 559

BLAST of MC03g0378 vs. TAIR 10
Match: AT2G24820.1 (translocon at the inner envelope membrane of chloroplasts 55-II )

HSP 1 Score: 184.9 bits (468), Expect = 1.8e-46
Identity = 140/511 (27.40%), Postives = 224/511 (43.84%), Query Frame = 0

Query: 61  PTPPSTADSEKSAVENSDEVEVDESGNESSSSP----------FSWRDNWYPVSLIEDLD 120
           PT P      +SAV  +   +  E G +   +P          + W + WYP+ L +++ 
Sbjct: 39  PTKPRLRLLRRSAVAGTAVSDQTEGGGDVLLNPEEEKRVEVADYDWTEEWYPLYLTKNVP 98

Query: 121 PALPTPFQLLGRDIVLWFDKSRGEWVAFDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFD 180
              P    +  R IVL+ D   G    ++D+CPHRLA LSEG++ + G L+C YHGW F+
Sbjct: 99  EDAPLGLTVYDRQIVLYKD-GEGTLRCYEDRCPHRLAKLSEGQLID-GRLECLYHGWQFE 158

Query: 181 GCGSCVKIPQASSEGPESRAHQSPRACATRFPTLVSQGLLFVWPDENGWERVNATAPPRL 240
           G G CVKIPQ  +     +A     AC   +    SQG+++VW             P +L
Sbjct: 159 GEGKCVKIPQLPASAKIPKA-----ACVKTYEVKDSQGVVWVWMSTK-----TPPNPEKL 218

Query: 241 P--DDFDKPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKV--TGRRDRAKPLPFKL 300
           P  ++F +P F  ++   +L Y +  L+EN+ DP+H+  +H +   T +R+ A+PL F++
Sbjct: 219 PWFENFARPGFFDISTTHELPYDHSILLENLMDPAHVPISHDRTDFTAKREDAQPLVFEV 278

Query: 301 DSNGPWGFSGA-----NKGNPRISAKFVAPCYYINKVEIDTKLPVLGDQKWVIWICSFNV 360
                 GF+G        G      +F APC   N  E + K  V       +++C    
Sbjct: 279 TERSNRGFAGTWGREKEGGKGSNLLRFDAPCVLQNNREFEGKDGVKNYFSG-LFLCR--- 338

Query: 361 PMGPGKTRSIVCSARNFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLS 420
           P G GK+  IV      F  +   P    V+P+W  H  + KV++ DM  L  Q ++   
Sbjct: 339 PTGQGKSMLIV-----RFGVTKRSPL-VSVLPQWFWHQNACKVFEQDMGFLSSQNEVL-- 398

Query: 421 KAMEDSTDVNKEYTKITFTPTQADRLVLAFRNWLRRHGKGQPEWFG-------------- 480
             M++       Y  +      +D  V  +R W+ + G G P  FG              
Sbjct: 399 --MKEKVPTKDLYLNL----KSSDTWVAEYRKWMDKVGHGMPYHFGHRTISLPKVPPVVE 458

Query: 481 ---------ASSQQPLPSTV--LSKRQMLDRFEQHTLHCSSCRGAYKSFQTLQKLLIGAT 528
                     S+  P    +  +    + +R+ +H +HC SC    KSF+  + +L    
Sbjct: 459 HAPAGLIAALSASYPAKGGIGTMHAPNLANRYFRHIIHCRSCSNVIKSFELWKNILSATA 518

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYC26.4e-23570.44Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAO P... [more]
Q0DV663.7e-22769.39Pheophorbide a oxygenase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947... [more]
Q8W4967.1e-6931.17Protochlorophyllide-dependent translocon component 52, chloroplastic OS=Arabidop... [more]
Q9SK502.5e-4527.40Protein TIC 55, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC55 PE=1 SV=1[more]
O499313.6e-4427.39Protein TIC 55, chloroplastic OS=Pisum sativum OX=3888 GN=TIC55 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022152935.10.0100.00pheophorbide a oxygenase, chloroplastic [Momordica charantia][more]
XP_022964963.10.093.60pheophorbide a oxygenase, chloroplastic [Cucurbita moschata][more]
XP_008462467.10.092.14PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo] >KAA0032593.1 ... [more]
XP_022970307.10.093.24pheophorbide a oxygenase, chloroplastic [Cucurbita maxima][more]
XP_023519435.10.093.25pheophorbide a oxygenase, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DFC60.0100.00pheophorbide a oxygenase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1HMG20.093.60pheophorbide a oxygenase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5D3DBC90.092.14Pheophorbide a oxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A6J1HYR30.093.24pheophorbide a oxygenase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A1S3CII70.092.14pheophorbide a oxygenase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500813 ... [more]
Match NameE-valueIdentityDescription
AT3G44880.14.5e-23670.44Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain [more]
AT4G25650.12.4e-7232.30ACD1-like [more]
AT4G25650.25.1e-7031.17ACD1-like [more]
AT2G24820.11.8e-4627.40translocon at the inner envelope membrane of chloroplasts 55-II [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017941Rieske [2Fe-2S] iron-sulphur domainPFAMPF00355Rieskecoord: 99..182
e-value: 6.0E-20
score: 71.0
IPR017941Rieske [2Fe-2S] iron-sulphur domainPROSITEPS51296RIESKEcoord: 100..212
score: 30.128016
IPR013626Pheophorbide a oxygenasePFAMPF08417PaOcoord: 308..402
e-value: 3.2E-23
score: 81.7
NoneNo IPR availableGENE3D3.90.380.10coord: 238..443
e-value: 1.8E-10
score: 42.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..94
NoneNo IPR availablePANTHERPTHR21266IRON-SULFUR DOMAIN CONTAINING PROTEINcoord: 17..547
NoneNo IPR availablePANTHERPTHR21266:SF24PHEOPHORBIDE A OXYGENASE, CHLOROPLASTICcoord: 17..547
NoneNo IPR availableCDDcd03480Rieske_RO_Alpha_PaOcoord: 88..223
e-value: 2.58113E-87
score: 263.799
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 207..443
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilyGENE3D2.102.10.10coord: 95..237
e-value: 7.1E-40
score: 137.8
IPR036922Rieske [2Fe-2S] iron-sulphur domain superfamilySUPERFAMILY50022ISP domaincoord: 94..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0378.1MC03g0378.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0031967 organelle envelope
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0010277 chlorophyllide a oxygenase [overall] activity
molecular_function GO:0046872 metal ion binding