MC03g0349 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0349
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionP-loop NTPase domain-containing protein LPA1 homolog 1-like
LocationMC03: 10034610 .. 10039122 (-)
RNA-Seq ExpressionMC03g0349
SyntenyMC03g0349
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGCGATGGCTGAGCTTCCCAAATTGCTCTACGTAGTAGTGGTGGATGCGGGAGACAAGCCGGAGAACGGGAAAGGGTCTTTCAGGTATACCCGTCATGGGCTGCAGAGCACTCTGCAGCTCATGGGTTGCAAACCGCGGCACGCTTTCAAGGTAATTCTTGTTTTGTTTTATCGAATTTGTTCAGGTAGATCCATTCTTAGGATGCAGATGCTTCAATTACTCTTTCCAGCTGTGATGATTGGTTGTGTTTCTTTGGATATTTTCATGTGGGAAACAGTTTTTAAATACCCTGGTCTTATTGGTCTGTTGTTATGCTGATTTAACGTTTTTCCTGCTGCTTCCTAGGTTTTGGAGGTGGGTTTGAATTTATATACTTCCAGATTCCATGTGGATTCTTCTGCAAATTTAACAGTTGAATTTTCTAACTAGCAAGTTTGTCTTCAACAGGGTTGTTAAGACGTGTCATTGGAGATTTACCTGTGGAACTTACAAGATTTAAACTAGGATGTAGAATTTCAATTATAATTGGGAAATCCGATTAAAATTAATTGACTAATCTGAGGACTTCATGTTCAACTCGGAGTCTGATTATCAATTATAATCCAAAGAGCTTAACCAGGGATTAAAAACAGAAACAATAAAATAGAACCCCAAGAATAATAAAGTATACAAAGATACAATATATTTATGTATTTTAGTGTTACCTTTAGCCCTGTATTACATTGTTGTGTGCATTAGGTAATGTGATTAATTTATTGCCATGGTACTCAGATCTTCTGGTAGCAAAAATGTTTCTGAGAGTAAAGATTGAAATATCTCAAGTTTGGGTTCCTATCAGTTTCACGGCTGAGAGATATCAGATTGTGAAAACCAATGGTGCTTTTGTCAGTTCTTTTCTTTCAATTTTCTCTACCTTTGATTTAGTCGTTGAGCAAGTCTTTTATCTTTGTGGCTAGGATGTTGGGTAGGTTGTAATTTCCTTAATTGCCTTTTTCGTTTGCTTTTTTATTTCTTAATGGTCAATCTTATTTGGTTTCCATTTGAAAGCCATACATCCCTGACTTGAAATGATTGAAATTTATGCTGAAAGAGTTTTGTTTTTCTAAACGCATTCTTCTGTGGCCAGATAAGCAAATGGGTATTTGAATTAATAAGAAAGGAAGATTCAACTGATGCTTTTCTCCCTGAAGACTTGGACATGTTGGGATCAGTTTCTTTGGTTGGAAAAACTGAAATAGATAGTAATCACTACTATGATTGCTTAGATGAAGAGGAGATTTTCAATAATTTGGGTTCAGGAGAAGGTGATAATTGCAAGAATAATCCATTCGAGTTATATAAAAGAAGCAGGACTGCTGTTGTTAGAAGAGAAACTTTCTTGGATGTTATTTGTGATTCTCTGGCCAAGTACAAGTACGTTGGTCCCAATCAGAGGGCAGACCTGATGTTGGCGTGCAGGTACTTAAAAAGTCTCATTTTTTCATCAAATCATATGATTTAAGAATCAAAATTTTGATGTAAATTTATATTTATATATTGCAGTGAGAATATTAATTTGGTTAGAAATTGGATATTTGTGCATGTTCTGTTGTAAGATGCAGAGAACACTTTTCATACTTATTTCTCATGCATTTGAACATGTTCATCAACAAATTTGTAGCATGTACTGCAGCTTGTTTGATAGTATTGATGTTTATCTCTGACTGAGTTTAAAGGTGGCTTTCTAAGAAGCTAGATCCAATAAGAAGCCCTTAGATGCAGCCTATCGAGTTGATAGTCTAAGTGTATAATGAGAGGTGCTTAAATTTTCTGGAATGTGTCCTCACAATAAGAAATGTCCAAGCTGCCAACACATATATGGGTCTTTCTTATATTTCCAATTAGATTCTTGTCTGTTTATCGTTTGCTCCACTGCCAGTCATCAAAGCCACATTTGCTTTCTGAAAATAGTAAACGATATTGTTCTTGATACATGCAAAATTATTAAACAGAATCAATGTTGAGAACACTCAGAATTGCAAGACACATTCTACTGTAACTTAACATCATCTGTTGGCCGTGTATATGGAGCGTGACAATATTTAAGCAATAAGCTCCTAATGATGTATCTAGAAAGGGTCATTTATTTCATTCTCTTTCTGTGTGGTGCACATTGCATTACATAATATCCTTTATTTAAAATTTCTCACTGCAGAATCCGAGAAAGGAAGGAGTCTGTAACTGTGCTATTATGTGGTACTAGCGGCTGTGGCAAATCCACTTTGTCAGCGCTGCTGGTAACTTTTTGTTTTCCTGATATCTATCTTTATATCCCAGAATTGTTGAACTTATTAAGTGAGACCCTTGGTTAATGTCACATATCACAACGAAGTAATTTCAATTTGTGACATTTATTTGCATGCTAAAATTTCTGGAATTTGAGCATTCTGCACTTCCCACTATAGGGTAGTAGGTTGGGAATTACAACAGTGGTTTCAACTGACTCTATACGACACATGATGAGGAGCTTTGTGGATGATAAACAAAATCCTTTGCTCTGGGCATCAACCTACCATGCTGGGGAATTTTTGGATCCTGTGGCTGTTGCTGAATCTAAGGCTAGGAAGAAAGCAAAAAAATTGGCTGGAATTTCTCACAAGCACCTGAAAGATGAAAGTGCTGATGGTTTGATGACCAAGAACTCTGACAGCCTATTAATAGGGTCAACGAAAGTTCCGAACAATTTGGAATTAATCAGTCCAAAGCAGATGGCTATTGAAGGATTTAAAGCGCAGAGTGAGATGGTGATAGATAGTCTTGATCGTCTTATTACTGCATGGGAAGAGCGGAAAGAATCTGTAATTGTTGAGGGAGTTCACTTAAGCTTGAGTTTTGTGGTATGGTTTGTATACATGACTTGTGTATCACTTCTTTATTATAGCTAATTTCTTTACATCCACTTTCTTTTCCAAAAAACAATTATAATCCTGATCTGCAGATGGGACTAATGAAGAAACACCCTTCAATTATACCATTCATGATCTATATAATGAATGAGGAAAAACACTTGGAACGATTTGCAGTACGTGCCAAATATATGGCATTGGACCCAGCAAAAAACAAGTATGTGAAGTATATTCGAAATATTAGAACAATTCAGGACTATCTCTGCAAGCGGGCTGACAAGCATCTTGTTCCCAAAATAAACAACACCAATGTTGATAAAAGCGTTGCTGCCATTCAAGCAACATTGTTCAGCTGCCTTCGTAGGCGTGAGGCTGGGGAGCAGCTTTATGATCCCTCCAGAAATACTGTTAGTGTAGTCGATGAGGAGTACAGGAACCAGTGTGTAGCGTATTCATTGAGTTCCAAGGGTATGTTTCAGATGATCCAAAGGAAAGGTTCTAAGCATTTGATGGCTCTTGTTAATACTGATGGATCGGTGGCAAAGGCTTGGCCCGTTGATTCAGTAGATAGCAATGGTAAACCTCTTCTGGGCCTCAAGACAGATGATAATGTAGGGAATTGGATGCGTGGATCACTTAATATAGGTAAGGTTGAACCAGTTAATCTTCAATTTGGTCTTTATGGCATCAGTGCTTGGCCAAGTAGCAGTGGCCCTAGTCATGCTAGTAGTCTTGATGAGTCGAGAGCTGAATGGACTGATACTGGTAGTAGATACTTCTCATCTTGCTGCAGTTCCCCTAGGATATTAGATGGACCTTCGAAGGAGGTAAATTTTCTATGTCAATTTTCCTTGCAACATTCAGTTACTAATTTTCTCTCCTTACTGGGACTTTGATAATATCTTGATGCATAAAATTGATATTTCCCTACAGCTCAAGGAGGATAATTCAGTTCATGGTAGTGATGACGAGGTTGACGATCCACATGAGTCTGGTAGTGATGAAGACATTAGTGATGATGGTGACAAGATGTTCCATGAAGAGGTAATATGATGGTAATATGTATTGCCATATGCCTGGCTTTTTTCATATCAGAATACTAGAATCGGCTTGAGTCCTCTTTTAAGAACCATATGGCAATTGAGTATCTTTTCATTTATTATTCCATACTATTATCATCTGGTGCAAGATTATAAAATATGATTTAGCCTTAAAACAGAAGTAGAATATGATTTAGCCTTTAAATATAAGTACTCCCCAATCCTCTTCTTTTACGTGCACAAAAATTTTTAATACATCTGAGGCAGTACAATAGTTTATTTTTATTTCATTCATTATTATTTTTAATGATGCAGATAGGATCAGTGGATGAGGAATCTACGAAATCAGATGAAGAATATGATGATCTGGAATTGCAGGACATCCTAGAGAGTGGCTATCAATCAGATGAAGATGATGAGGAGGTTAAAGGAAAATTTGAATATGATTTAATGGACCAATTTAGACTCAAAAAAGGATTTAAGCATAGCCAAAACCTGGATCCATTCCCACAGAGTAAGAGAGGGACATTTGATCCCCTCCACTCTTATTCATCTATGCATGCAGAGAAGAATGGAGCAGTTTGC

mRNA sequence

ACAGCGATGGCTGAGCTTCCCAAATTGCTCTACGTAGTAGTGGTGGATGCGGGAGACAAGCCGGAGAACGGGAAAGGGTCTTTCAGGTATACCCGTCATGGGCTGCAGAGCACTCTGCAGCTCATGGGTTGCAAACCGCGGCACGCTTTCAAGATAAGCAAATGGGTATTTGAATTAATAAGAAAGGAAGATTCAACTGATGCTTTTCTCCCTGAAGACTTGGACATGTTGGGATCAGTTTCTTTGGTTGGAAAAACTGAAATAGATAGTAATCACTACTATGATTGCTTAGATGAAGAGGAGATTTTCAATAATTTGGGTTCAGGAGAAGGTGATAATTGCAAGAATAATCCATTCGAGTTATATAAAAGAAGCAGGACTGCTGTTGTTAGAAGAGAAACTTTCTTGGATGTTATTTGTGATTCTCTGGCCAAGTACAAGTACGTTGGTCCCAATCAGAGGGCAGACCTGATGTTGGCGTGCAGAATCCGAGAAAGGAAGGAGTCTGTAACTGTGCTATTATGTGGTACTAGCGGCTGTGGCAAATCCACTTTGTCAGCGCTGCTGGGTAGTAGGTTGGGAATTACAACAGTGGTTTCAACTGACTCTATACGACACATGATGAGGAGCTTTGTGGATGATAAACAAAATCCTTTGCTCTGGGCATCAACCTACCATGCTGGGGAATTTTTGGATCCTGTGGCTGTTGCTGAATCTAAGGCTAGGAAGAAAGCAAAAAAATTGGCTGGAATTTCTCACAAGCACCTGAAAGATGAAAGTGCTGATGGTTTGATGACCAAGAACTCTGACAGCCTATTAATAGGGTCAACGAAAGTTCCGAACAATTTGGAATTAATCAGTCCAAAGCAGATGGCTATTGAAGGATTTAAAGCGCAGAGTGAGATGGTGATAGATAGTCTTGATCGTCTTATTACTGCATGGGAAGAGCGGAAAGAATCTGTAATTGTTGAGGGAGTTCACTTAAGCTTGAGTTTTGTGATGGGACTAATGAAGAAACACCCTTCAATTATACCATTCATGATCTATATAATGAATGAGGAAAAACACTTGGAACGATTTGCAGTACGTGCCAAATATATGGCATTGGACCCAGCAAAAAACAAGTATGTGAAGTATATTCGAAATATTAGAACAATTCAGGACTATCTCTGCAAGCGGGCTGACAAGCATCTTGTTCCCAAAATAAACAACACCAATGTTGATAAAAGCGTTGCTGCCATTCAAGCAACATTGTTCAGCTGCCTTCGTAGGCGTGAGGCTGGGGAGCAGCTTTATGATCCCTCCAGAAATACTGTTAGTGTAGTCGATGAGGAGTACAGGAACCAGTGTGTAGCGTATTCATTGAGTTCCAAGGGTATGTTTCAGATGATCCAAAGGAAAGGTTCTAAGCATTTGATGGCTCTTGTTAATACTGATGGATCGGTGGCAAAGGCTTGGCCCGTTGATTCAGTAGATAGCAATGGTAAACCTCTTCTGGGCCTCAAGACAGATGATAATGTAGGGAATTGGATGCGTGGATCACTTAATATAGGTAAGGTTGAACCAGTTAATCTTCAATTTGGTCTTTATGGCATCAGTGCTTGGCCAAGTAGCAGTGGCCCTAGTCATGCTAGTAGTCTTGATGAGTCGAGAGCTGAATGGACTGATACTGGTAGTAGATACTTCTCATCTTGCTGCAGTTCCCCTAGGATATTAGATGGACCTTCGAAGGAGCTCAAGGAGGATAATTCAGTTCATGGTAGTGATGACGAGGTTGACGATCCACATGAGTCTGGTAGTGATGAAGACATTAGTGATGATGGTGACAAGATGTTCCATGAAGAGATAGGATCAGTGGATGAGGAATCTACGAAATCAGATGAAGAATATGATGATCTGGAATTGCAGGACATCCTAGAGAGTGGCTATCAATCAGATGAAGATGATGAGGAGGTTAAAGGAAAATTTGAATATGATTTAATGGACCAATTTAGACTCAAAAAAGGATTTAAGCATAGCCAAAACCTGGATCCATTCCCACAGAGTAAGAGAGGGACATTTGATCCCCTCCACTCTTATTCATCTATGCATGCAGAGAAGAATGGAGCAGTTTGC

Coding sequence (CDS)

ACAGCGATGGCTGAGCTTCCCAAATTGCTCTACGTAGTAGTGGTGGATGCGGGAGACAAGCCGGAGAACGGGAAAGGGTCTTTCAGGTATACCCGTCATGGGCTGCAGAGCACTCTGCAGCTCATGGGTTGCAAACCGCGGCACGCTTTCAAGATAAGCAAATGGGTATTTGAATTAATAAGAAAGGAAGATTCAACTGATGCTTTTCTCCCTGAAGACTTGGACATGTTGGGATCAGTTTCTTTGGTTGGAAAAACTGAAATAGATAGTAATCACTACTATGATTGCTTAGATGAAGAGGAGATTTTCAATAATTTGGGTTCAGGAGAAGGTGATAATTGCAAGAATAATCCATTCGAGTTATATAAAAGAAGCAGGACTGCTGTTGTTAGAAGAGAAACTTTCTTGGATGTTATTTGTGATTCTCTGGCCAAGTACAAGTACGTTGGTCCCAATCAGAGGGCAGACCTGATGTTGGCGTGCAGAATCCGAGAAAGGAAGGAGTCTGTAACTGTGCTATTATGTGGTACTAGCGGCTGTGGCAAATCCACTTTGTCAGCGCTGCTGGGTAGTAGGTTGGGAATTACAACAGTGGTTTCAACTGACTCTATACGACACATGATGAGGAGCTTTGTGGATGATAAACAAAATCCTTTGCTCTGGGCATCAACCTACCATGCTGGGGAATTTTTGGATCCTGTGGCTGTTGCTGAATCTAAGGCTAGGAAGAAAGCAAAAAAATTGGCTGGAATTTCTCACAAGCACCTGAAAGATGAAAGTGCTGATGGTTTGATGACCAAGAACTCTGACAGCCTATTAATAGGGTCAACGAAAGTTCCGAACAATTTGGAATTAATCAGTCCAAAGCAGATGGCTATTGAAGGATTTAAAGCGCAGAGTGAGATGGTGATAGATAGTCTTGATCGTCTTATTACTGCATGGGAAGAGCGGAAAGAATCTGTAATTGTTGAGGGAGTTCACTTAAGCTTGAGTTTTGTGATGGGACTAATGAAGAAACACCCTTCAATTATACCATTCATGATCTATATAATGAATGAGGAAAAACACTTGGAACGATTTGCAGTACGTGCCAAATATATGGCATTGGACCCAGCAAAAAACAAGTATGTGAAGTATATTCGAAATATTAGAACAATTCAGGACTATCTCTGCAAGCGGGCTGACAAGCATCTTGTTCCCAAAATAAACAACACCAATGTTGATAAAAGCGTTGCTGCCATTCAAGCAACATTGTTCAGCTGCCTTCGTAGGCGTGAGGCTGGGGAGCAGCTTTATGATCCCTCCAGAAATACTGTTAGTGTAGTCGATGAGGAGTACAGGAACCAGTGTGTAGCGTATTCATTGAGTTCCAAGGGTATGTTTCAGATGATCCAAAGGAAAGGTTCTAAGCATTTGATGGCTCTTGTTAATACTGATGGATCGGTGGCAAAGGCTTGGCCCGTTGATTCAGTAGATAGCAATGGTAAACCTCTTCTGGGCCTCAAGACAGATGATAATGTAGGGAATTGGATGCGTGGATCACTTAATATAGGTAAGGTTGAACCAGTTAATCTTCAATTTGGTCTTTATGGCATCAGTGCTTGGCCAAGTAGCAGTGGCCCTAGTCATGCTAGTAGTCTTGATGAGTCGAGAGCTGAATGGACTGATACTGGTAGTAGATACTTCTCATCTTGCTGCAGTTCCCCTAGGATATTAGATGGACCTTCGAAGGAGCTCAAGGAGGATAATTCAGTTCATGGTAGTGATGACGAGGTTGACGATCCACATGAGTCTGGTAGTGATGAAGACATTAGTGATGATGGTGACAAGATGTTCCATGAAGAGATAGGATCAGTGGATGAGGAATCTACGAAATCAGATGAAGAATATGATGATCTGGAATTGCAGGACATCCTAGAGAGTGGCTATCAATCAGATGAAGATGATGAGGAGGTTAAAGGAAAATTTGAATATGATTTAATGGACCAATTTAGACTCAAAAAAGGATTTAAGCATAGCCAAAACCTGGATCCATTCCCACAGAGTAAGAGAGGGACATTTGATCCCCTCCACTCTTATTCATCTATGCATGCAGAGAAGAATGGAGCAGTTTGC

Protein sequence

TAMAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRKEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMDQFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC
Homology
BLAST of MC03g0349 vs. ExPASy Swiss-Prot
Match: Q9FJH9 (P-loop NTPase domain-containing protein LPA1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At5g60760 PE=2 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 1.6e-230
Identity = 454/718 (63.23%), Postives = 537/718 (74.79%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPE--------NGKGSFRYTRHGLQSTLQLMGCKPRHAFKISK 62
           M E  K++Y+VVVD  D  E          K SFRYTR  LQSTLQLMGCK RHAFKIS+
Sbjct: 1   MTETTKVMYIVVVDGTDTAETVEEDGTGTWKDSFRYTRPVLQSTLQLMGCKARHAFKISR 60

Query: 63  WVFELIRKEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYD---CLDEEEI--FNNLGSG 122
            VFELIR E S        L +  S+S     E +     D   C + E+    N+L + 
Sbjct: 61  RVFELIRSEGS--------LILSPSLSPSHSKESEFQKTGDASACANVEKANKVNSLATD 120

Query: 123 EGDNCKNNPFELYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKES 182
           + D  K+ PFE+YKR  T VV RE F++V+CD+LA+YKYVG +QRADL+L+CRIRERKES
Sbjct: 121 DVDKSKSKPFEVYKRRTTVVVSREIFVNVVCDALAEYKYVGHDQRADLILSCRIRERKES 180

Query: 183 VTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGE 242
           VTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVD+KQNPLLWASTYHAGE
Sbjct: 181 VTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDEKQNPLLWASTYHAGE 240

Query: 243 FLDPVAVAESKA-RKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISP 302
           +LDPVAVAESKA R++AKK+  I  +  K ++++G   KN+    +GSTK  N   L+SP
Sbjct: 241 YLDPVAVAESKARRRRAKKMDSIEDE--KAKASEGGKAKNTQQTDVGSTK--NTPVLLSP 300

Query: 303 KQMAIEGFKAQSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMI 362
           KQMA+EGFKAQSEMVID+LDRLITAWEERKESVIVEGVHLSL+FVMGLMKKHPSI+PFM+
Sbjct: 301 KQMAVEGFKAQSEMVIDNLDRLITAWEERKESVIVEGVHLSLNFVMGLMKKHPSIVPFMV 360

Query: 363 YIMNEEKHLERFAVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVD 422
           YI NEEKHLERFAVRAKYM LDP KNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVD
Sbjct: 361 YIANEEKHLERFAVRAKYMTLDPEKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVD 420

Query: 423 KSVAAIQATLFSCLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG 482
           KSVAAI AT+FSCLRRREAGE LYD + NTVSV+D+EYRNQC A SLSSKGMFQ+IQRKG
Sbjct: 421 KSVAAIHATVFSCLRRREAGEHLYDATTNTVSVIDDEYRNQCTANSLSSKGMFQLIQRKG 480

Query: 483 -SKHLMALVNTDGSVAKAWPV-DSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQ 542
            S+HLMAL+NTDG+ A+ WPV   VD +GKP+   +  +  G        + K EPVNLQ
Sbjct: 481 SSRHLMALLNTDGTFARTWPVTGKVDESGKPVFCNEMIEENGMEHPVYGYLQKAEPVNLQ 540

Query: 543 FGLYGISAWPSSSGPSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVH 602
           FGL+GISAWPS    S A S+D+ +A+  +T SRY+SSCCSSPR+ +G SKELKED SVH
Sbjct: 541 FGLFGISAWPSDGATSRAGSVDDCKADMAETSSRYYSSCCSSPRMSEGTSKELKEDQSVH 600

Query: 603 GSDDEV-DDPHESGSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQS 662
           GSD+EV DDP E   D D SDD +K  H+E+GSVDE+STKSDEEYDDL ++D     Y +
Sbjct: 601 GSDEEVEDDPPE--PDTDFSDDDNKRDHDEVGSVDEQSTKSDEEYDDLAMED---KSYWT 660

Query: 663 DEDDEEVKGKFEYDLMDQFRLKKGFKHSQNLDPFPQ-SKRGTFDPLHSYSSMHAEKNG 703
           DE++E           +  +  K  K+ QNLD F + + +   +PL   +S+   +NG
Sbjct: 661 DEEEESRDTIAIMSEKNNKQATKEDKYIQNLDLFLRTANQQLVEPLQLCASLLTCENG 701

BLAST of MC03g0349 vs. ExPASy Swiss-Prot
Match: Q93ZS1 (P-loop NTPase domain-containing protein LPA1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At3g45090 PE=2 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 8.2e-227
Identity = 435/687 (63.32%), Postives = 513/687 (74.67%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPE-NGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIR 62
           M E  K+LY+VV + GD  + NG  SFRYTR  LQSTLQLMGCK RHAFKIS+ VFELIR
Sbjct: 2   MTEATKVLYIVVREEGDDDDNNGDDSFRYTRPVLQSTLQLMGCKARHAFKISRRVFELIR 61

Query: 63  KEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFEL 122
            E S +       +   +  + G T ++     +CL        + +G+ D  K+ PFE+
Sbjct: 62  SEGSCNTSPENGKEPEFAKEVGGSTCVEK---LNCL--------VVAGDVDKNKSKPFEM 121

Query: 123 YKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCG 182
           YKR  T VV RE F+DV+CD+LA+YKYVG +QRADL+LACRIRERKESVTVLLCGTSGCG
Sbjct: 122 YKRRTTVVVSREIFVDVVCDALAEYKYVGRDQRADLILACRIRERKESVTVLLCGTSGCG 181

Query: 183 KSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKA 242
           KSTLSALLGSRLGITTVVSTDSIRHMMRSF D+KQNPLLWASTYHAGE+LDPVAVAESKA
Sbjct: 182 KSTLSALLGSRLGITTVVSTDSIRHMMRSFADEKQNPLLWASTYHAGEYLDPVAVAESKA 241

Query: 243 RKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSE 302
           ++KAKKL G    +   +  D            GS    +  EL+S KQMAIEG+KAQSE
Sbjct: 242 KRKAKKLKGSRGVNSNAQKTDA-----------GSNS--STTELLSHKQMAIEGYKAQSE 301

Query: 303 MVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFA 362
           MVIDSLDRLIT WEER ESV+VEGVHLSL+FVMGLMKKHPSI+PFM+YI NEEKHLERFA
Sbjct: 302 MVIDSLDRLITTWEERNESVVVEGVHLSLNFVMGLMKKHPSIVPFMVYIANEEKHLERFA 361

Query: 363 VRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSC 422
           VRAKYM LDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVA I AT+F C
Sbjct: 362 VRAKYMTLDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVATIHATVFGC 421

Query: 423 LRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG-SKHLMALVNTDG 482
           LRRRE GE+LYD + NTVSV+D+E+RNQC A SL+SKGMFQ+IQR+G S+  MAL NTDG
Sbjct: 422 LRRRETGEKLYDTTTNTVSVIDDEHRNQCAANSLTSKGMFQVIQRQGSSRRFMALCNTDG 481

Query: 483 SVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG 542
           +VAK WPV SV    KP++  + DD   +       + K EPVNLQFG +GISAWPS   
Sbjct: 482 TVAKTWPVASVGKIRKPVVNTEMDDGTEH------QLHKAEPVNLQFGHFGISAWPSDGA 541

Query: 543 PSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDE----VDDPH 602
            SHA S+D+ RA+  +TGSR++SSCCSSPR  DGPSKEL E+ SV+GSD++     DD H
Sbjct: 542 TSHAGSVDDLRADIIETGSRHYSSCCSSPRTSDGPSKELMEEQSVNGSDEDDEEGDDDFH 601

Query: 603 ESGSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKF 662
           E  SDED+SD+ D+   +EIGSVDEESTKSDEEYDDL ++D     Y +D ++EE +   
Sbjct: 602 EPDSDEDLSDNNDERNRDEIGSVDEESTKSDEEYDDLAMED---KSYWTDNEEEESRDTI 655

Query: 663 ----EYDLMDQFRLKKGFKHSQNLDPF 680
               + +  +  +  K  K+SQNLD F
Sbjct: 662 SMVSQNNHNEASKTNKDDKYSQNLDLF 655

BLAST of MC03g0349 vs. ExPASy Swiss-Prot
Match: B9F4I8 (P-loop NTPase domain-containing protein LPA1 OS=Oryza sativa subsp. japonica OX=39947 GN=LPA1 PE=2 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 2.5e-215
Identity = 415/646 (64.24%), Postives = 494/646 (76.47%), Query Frame = 0

Query: 7   PKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRKEDST 66
           PKLLY+ V DA       + +FRYTR  LQ TLQLMGCK RHAFKISK VF ++R E   
Sbjct: 41  PKLLYIAVSDAA-----ARRAFRYTRPVLQGTLQLMGCKARHAFKISKRVFNVMRSE-FL 100

Query: 67  DAFLPEDLD-MLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELYKRS 126
           DA   +  D    + SLV   E+        L  + +   L S         PFELYK  
Sbjct: 101 DASKSDTADNEENAPSLVKDVEM--------LKPKILEATLSS--------IPFELYKTQ 160

Query: 127 RTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGKSTL 186
            T VV RE FL V+CD+L+ YKYVGPNQ+AD +LACRI+ERKESVTVLLCGTSGCGKSTL
Sbjct: 161 TTIVVSREKFLSVVCDALSSYKYVGPNQKADFLLACRIKERKESVTVLLCGTSGCGKSTL 220

Query: 187 SALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKARKKA 246
           S+LLGSRLGITTVVSTDSIRHMMR F D+KQNPLL+ASTYHAGE LDPVAVA++KA++KA
Sbjct: 221 SSLLGSRLGITTVVSTDSIRHMMRGFTDEKQNPLLYASTYHAGECLDPVAVAQAKAKRKA 280

Query: 247 KKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEMVID 306
           +KL  +SH +  +   D     + D    GS+++P   ELI  KQMAIEGFKAQSEMVID
Sbjct: 281 QKLDIVSHPNTNEGRDD----TSDDKAHHGSSELPPRTELIGSKQMAIEGFKAQSEMVID 340

Query: 307 SLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAVRAK 366
           SLDRLIT+WEE+K+SVIVEGVHLSL+FVMGLMKKHPSIIPFM+YI NEEKH+ERFAVRAK
Sbjct: 341 SLDRLITSWEEQKQSVIVEGVHLSLNFVMGLMKKHPSIIPFMVYIANEEKHMERFAVRAK 400

Query: 367 YMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCLRRR 426
           YM LDPAKN+Y+KYIRNIR IQDYLC RADKHLVPKINNTNVD+SVAAI AT+FSCLRRR
Sbjct: 401 YMTLDPAKNRYIKYIRNIRAIQDYLCNRADKHLVPKINNTNVDQSVAAIHATVFSCLRRR 460

Query: 427 EAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG-SKHLMALVNTDGSVAK 486
           EAGEQLYD + NTV+VV+EEYRNQ  A SL SKGMFQ+IQR+G S++LMA++NTDGSV K
Sbjct: 461 EAGEQLYDLNTNTVAVVNEEYRNQRAANSLGSKGMFQLIQRQGSSRNLMAILNTDGSVTK 520

Query: 487 AWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPS-SSGPSH 546
           AW VD  + NG  L G  +D +  N M  +   GK EPVNLQFG +GISAW S + G SH
Sbjct: 521 AWHVDKNNGNGS-LDGTSSDKSTKNPMYDT--FGKAEPVNLQFGSFGISAWMSDTGGTSH 580

Query: 547 ASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDN-SVHGSDDEVDDPHESGSDE 606
             S+D+ RA+  +TG RY+SSCCSSP++ D PSKEL ED+ SV GS+++ DDP ++G+DE
Sbjct: 581 TGSVDDLRADGIETGGRYYSSCCSSPKVSDCPSKELMEDDYSVFGSEEDADDPPDAGTDE 640

Query: 607 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDED 649
           D++D+   M   E GSVDE STKSDEEYDDL +QD++E+GY SD++
Sbjct: 641 DLTDEERDMHEIEAGSVDEHSTKSDEEYDDLAMQDVMENGYWSDDE 657

BLAST of MC03g0349 vs. ExPASy Swiss-Prot
Match: Q651A1 (P-loop NTPase domain-containing protein LPA1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0572200 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 2.7e-193
Identity = 381/650 (58.62%), Postives = 464/650 (71.38%), Query Frame = 0

Query: 8   KLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRKEDSTD 67
           KLLY+VVVD      +   +FRYTR  L STLQLMGCKPRHAF+IS  VF+ IR      
Sbjct: 11  KLLYIVVVD------DDGATFRYTRSLLHSTLQLMGCKPRHAFEISGRVFDEIRGH---- 70

Query: 68  AFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELYKRSRT 127
                   M G +++ G   +            + +      E  + +   FELYKR  T
Sbjct: 71  --------MGGDMAMGGGGGV------------QRYELAADAEAASPRQFQFELYKRRTT 130

Query: 128 AVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGKSTLSA 187
            ++ R  FL ++C +LA YKYV P+QR+DL  ACRIRERKESVT+LLCGTSGCGKSTLS 
Sbjct: 131 LLIPRPLFLRLVCHALALYKYVAPDQRSDLHRACRIRERKESVTILLCGTSGCGKSTLST 190

Query: 188 LLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKARKKAKK 247
           LLGSRLGITTVVSTDSIRHMMRSFV++KQNPLLWASTYHAGE LDPVAVA++KAR+KAKK
Sbjct: 191 LLGSRLGITTVVSTDSIRHMMRSFVEEKQNPLLWASTYHAGECLDPVAVADAKARRKAKK 250

Query: 248 LAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEMVIDSL 307
            +GIS     D      +    D   IG             KQMAIEG+KAQSEMVIDSL
Sbjct: 251 RSGISTTSTIDFDKTRPLNDKPDGKPIGK------------KQMAIEGYKAQSEMVIDSL 310

Query: 308 DRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAVRAKYM 367
           DRLITAWE+RKESV+VEGVHLSL+FVMGLM+KHPSIIPFMIYI +E KH ERFAVRAKYM
Sbjct: 311 DRLITAWEDRKESVVVEGVHLSLNFVMGLMRKHPSIIPFMIYISDEGKHTERFAVRAKYM 370

Query: 368 ALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCLRRREA 427
            LDP KNKYVKYI NIRTIQ+YLC RADK+LVPK+NNTNVD+SVA+I AT+FSCLRRR A
Sbjct: 371 TLDPTKNKYVKYISNIRTIQEYLCSRADKYLVPKVNNTNVDRSVASIHATVFSCLRRRAA 430

Query: 428 GEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG-SKHLMALVNTDGSVAKAW 487
           G+QLYDP+ NTV+VV+EEY+NQCVA S+SSKGMF++IQR G S+ LMA+VN DGSV+KAW
Sbjct: 431 GDQLYDPATNTVAVVNEEYKNQCVANSMSSKGMFKLIQRLGSSRKLMAIVNVDGSVSKAW 490

Query: 488 PVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG-PSHAS 547
           PV+S   +GK      +   VG+ + G LNIG+ E VNLQFG +GISAWP+ +G  S A 
Sbjct: 491 PVESSSGDGKGGSENGSKKYVGDPIYGPLNIGRAESVNLQFGAFGISAWPTDAGCTSQAG 550

Query: 548 SLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDE-------VDDPHES 607
           S++ES     +    +  S   SP+ LDG  KE+KE  +  GSDD+        D P  S
Sbjct: 551 SVNESWDNANEGTGSHVPSSSGSPKKLDGHCKEIKESAAASGSDDDEEEEEEAADVPPNS 610

Query: 608 GSDEDISDDGDKMFHEEI-GSVDEESTKSDEEYDDLELQDILESGYQSDE 648
           GS+ED+S++  +  HEE+ GSVDE+  +SDEEYDDL ++D +E+G+ +D+
Sbjct: 611 GSEEDLSEEDIRAIHEEMEGSVDEDCNRSDEEYDDLAMRDCMENGFLTDD 618

BLAST of MC03g0349 vs. ExPASy Swiss-Prot
Match: Q8T1P1 (Uncharacterized protein DDB_G0273453/DDB_G0273565 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0273453 PE=4 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 1.6e-76
Identity = 178/507 (35.11%), Postives = 274/507 (54.04%), Query Frame = 0

Query: 29  RYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK--EDSTDAFLPEDLDMLGSVSLVGKT 88
           RY R  L  TL+ MGCK  HA +IS  VFE + K   +        + + + +   + KT
Sbjct: 124 RYARFHLNLTLKFMGCKSIHARQISNTVFEQLEKCRIEQNKQLTATNNNAIVTTDSITKT 183

Query: 89  EIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELYKRSRTAVVRRETFLDVICDSLAKY 148
           E  +                 +         P  +Y  S    ++R  F  +I   L+ Y
Sbjct: 184 EQSTT------TTTTTTTTTTTTTATTTTQPP--IYCVS----IQRNIFYYIIGHILSCY 243

Query: 149 KYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRH 208
           +Y  P    D  ++C ++++K S T+LL GTSGCGKSTL+ALL SR+G T V+STD+IR 
Sbjct: 244 QYSKPQYIIDFPVSCEVQDKKHSFTILLGGTSGCGKSTLTALLASRIGFTAVISTDNIRQ 303

Query: 209 MMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKARKKAKKLAGISHKHLKDESADGLMT 268
           ++R F+  +++P+LWASTYHAGE +   +                               
Sbjct: 304 LLRKFISRQESPILWASTYHAGEIISNPS------------------------------- 363

Query: 269 KNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEMVIDSLDRLITAWEERKESVIVEGV 328
                              +S K+  ++G++AQ+EM+ + LD LI  +E+RKES+IVEGV
Sbjct: 364 -------------------LSHKEKILQGYEAQNEMIFNKLDILIGHYEKRKESLIVEGV 423

Query: 329 HLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAVRAKYMALDPAKNKYVKYIRNIRTI 388
           HL    ++ L+KKHPS IPF++YI NE KH ERFA+R+KYM LDP +NKY KY +NIR I
Sbjct: 424 HLDTKLILRLVKKHPSCIPFLMYISNEAKHKERFAIRSKYMTLDPHQNKYTKYFKNIRII 483

Query: 389 QDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCLRRR-EAGEQLYDPSRNTVSVVDEE 448
            D+LC  AD+H++P+I+NT++D+S+A I  T+F+CL+R+ + GE  Y+   + ++++  +
Sbjct: 484 NDHLCHGADEHMIPQIDNTSIDRSLATIHGTIFACLKRKVQCGESYYNHETDKMNMLYNQ 543

Query: 449 YRNQCVAYSLSSKGMFQMIQRK------GSKHLMALVNTDGSVAKAWPV--DSVDSNGKP 508
           Y  Q      SSKGM ++IQ+K      G+ ++   V+ + S +    V  ++ D+NG  
Sbjct: 544 Y-EQIQHQFWSSKGMLRLIQKKKTVSPHGNNNVTGDVDNNNSNSNNNNVNNNNGDNNGDS 566

Query: 509 LLGLKTDDNVGNWMRGSLNIGKVEPVN 525
            +   ++DN  N    S NI  +   N
Sbjct: 604 NVDNNSNDNDNN-NNNSNNISNINNDN 566

BLAST of MC03g0349 vs. NCBI nr
Match: XP_022152872.1 (P-loop NTPase domain-containing protein LPA1 homolog 1-like [Momordica charantia])

HSP 1 Score: 1392 bits (3603), Expect = 0.0
Identity = 703/703 (100.00%), Postives = 703/703 (100.00%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 122
           EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY
Sbjct: 61  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 120

Query: 123 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 182
           KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK
Sbjct: 121 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 180

Query: 183 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 242
           STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR
Sbjct: 181 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 240

Query: 243 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 302
           KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM
Sbjct: 241 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 300

Query: 303 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 362
           VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV
Sbjct: 301 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 360

Query: 363 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 422
           RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL
Sbjct: 361 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 420

Query: 423 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 482
           RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV
Sbjct: 421 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 480

Query: 483 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 542
           AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS
Sbjct: 481 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 540

Query: 543 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 602
           HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE
Sbjct: 541 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 600

Query: 603 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 662
           DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD
Sbjct: 601 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 660

Query: 663 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC 705
           QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC
Sbjct: 661 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC 703

BLAST of MC03g0349 vs. NCBI nr
Match: XP_038896070.1 (P-loop NTPase domain-containing protein LPA1 homolog 1-like [Benincasa hispida])

HSP 1 Score: 1220 bits (3157), Expect = 0.0
Identity = 624/703 (88.76%), Postives = 659/703 (93.74%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAE+ KLLY+VVVD  +KPE GK SFRYTR+ LQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAEVAKLLYIVVVDEEEKPEKGKLSFRYTRYVLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 122
           E ST+ FLPE+ ++LGSVSL GKTE D  HYY+C D  E+FN  GS EGDN  N PFELY
Sbjct: 61  EASTENFLPEERNILGSVSLGGKTESDGIHYYNCFDGNEMFNQ-GSREGDNSNNIPFELY 120

Query: 123 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 182
           KRSRTAVVRRE FLDVICDSLAKYKYVGPNQRADL+LACRIRERKESVTVLLCGTSGCGK
Sbjct: 121 KRSRTAVVRREFFLDVICDSLAKYKYVGPNQRADLILACRIRERKESVTVLLCGTSGCGK 180

Query: 183 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 242
           STLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESKAR
Sbjct: 181 STLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESKAR 240

Query: 243 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 302
           K AKKLAGISHKHLKDE+ADGL+T+NSD+LLIGS KV NNLELISPKQMAIEGFKAQSEM
Sbjct: 241 KNAKKLAGISHKHLKDETADGLITRNSDNLLIGSAKVMNNLELISPKQMAIEGFKAQSEM 300

Query: 303 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 362
           VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERFAV
Sbjct: 301 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERFAV 360

Query: 363 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 422
           RAKYM LDPAKNKYVKYIRNIRTIQDYLC RADKHLVPKINNTNVDKSVAAIQATLFSCL
Sbjct: 361 RAKYMTLDPAKNKYVKYIRNIRTIQDYLCNRADKHLVPKINNTNVDKSVAAIQATLFSCL 420

Query: 423 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 482
           RRREAGEQLYD SRNTV+VVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMAL+NTDGSV
Sbjct: 421 RRREAGEQLYDSSRNTVTVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALLNTDGSV 480

Query: 483 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 542
           AKAWPVDSVDSNGKPLLGL+ DD+VGN M GSLNIGKVEPVNLQFGLYGISAWPSS GPS
Sbjct: 481 AKAWPVDSVDSNGKPLLGLEPDDSVGNLMLGSLNIGKVEPVNLQFGLYGISAWPSSGGPS 540

Query: 543 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 602
           HA S+DESRAE TDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSD+EVDDP ESGSDE
Sbjct: 541 HAGSVDESRAEGTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDEEVDDPTESGSDE 600

Query: 603 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 662
           D SDDGDKMFHEEIGSVDEESTKSDEEYDDL +QDIL+SGYQSDEDDE+VK K E+D MD
Sbjct: 601 DRSDDGDKMFHEEIGSVDEESTKSDEEYDDLAMQDILDSGYQSDEDDEKVKIKTEHDFMD 660

Query: 663 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC 705
           QFRL+KGFK+SQNL+PF QSK+GTF+PLHSYSS+H EKN AVC
Sbjct: 661 QFRLRKGFKYSQNLEPFQQSKKGTFEPLHSYSSIHTEKNEAVC 702

BLAST of MC03g0349 vs. NCBI nr
Match: KAA0051769.1 (P-loop NTPase domain-containing protein LPA1-like protein 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1189 bits (3077), Expect = 0.0
Identity = 612/704 (86.93%), Postives = 652/704 (92.61%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAE+ KLLY+VVVD  +KPE  K SFRYTRH LQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAEVAKLLYIVVVDEEEKPEKEKTSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 122
           E ST+ FLPE+ ++LGS+SL G TE D N YY+CLD +E FN  GS EGDN  N PFELY
Sbjct: 61  EASTENFLPEEWNVLGSISLGGNTESDGNQYYNCLDGKETFNQ-GSREGDNSNNIPFELY 120

Query: 123 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 182
           KRSRTAVV RE+FLDVICDSLAKYKYVGPNQRADL+LACRIRERKESVTVLLCGTSGCGK
Sbjct: 121 KRSRTAVVGRESFLDVICDSLAKYKYVGPNQRADLILACRIRERKESVTVLLCGTSGCGK 180

Query: 183 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 242
           STLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESKAR
Sbjct: 181 STLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESKAR 240

Query: 243 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 302
           KKAKKLAGISHKHLKDE+A+GL+++NSD+ L GS KV NNLELISPKQMAIEGFKAQSEM
Sbjct: 241 KKAKKLAGISHKHLKDETANGLISRNSDNPLTGSAKVTNNLELISPKQMAIEGFKAQSEM 300

Query: 303 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 362
           VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERFAV
Sbjct: 301 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERFAV 360

Query: 363 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 422
           RAKYM LDPAKN+YVKYIRNIRTIQDYLC RADKHLVPKINNTNVDKSVAAIQATLFSCL
Sbjct: 361 RAKYMTLDPAKNRYVKYIRNIRTIQDYLCNRADKHLVPKINNTNVDKSVAAIQATLFSCL 420

Query: 423 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 482
           RRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV
Sbjct: 421 RRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 480

Query: 483 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 542
           AKAWPVDSVD NGKPLLGLK DD+VGN M GSL IGKVEPVNLQFGLYGISAWPSSSGPS
Sbjct: 481 AKAWPVDSVDRNGKPLLGLKEDDSVGNLMLGSLQIGKVEPVNLQFGLYGISAWPSSSGPS 540

Query: 543 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 602
           HA S+DESRAE TDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSD+EVDDP ESGSDE
Sbjct: 541 HAGSVDESRAEGTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDEEVDDPPESGSDE 600

Query: 603 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 662
           D SDDG+K+FHEEIGSVDEESTKSDEEYDDL +QDIL+SGYQSDEDD+ VK K E+D MD
Sbjct: 601 DFSDDGNKIFHEEIGSVDEESTKSDEEYDDLAMQDILDSGYQSDEDDK-VKTKVEHDFMD 660

Query: 663 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEK-NGAVC 705
           +FRL KG K+SQ+L+PF Q+K+GTF+PLHSY S+H EK N AVC
Sbjct: 661 KFRLIKGLKYSQSLEPFQQNKKGTFEPLHSYPSIHTEKKNEAVC 702

BLAST of MC03g0349 vs. NCBI nr
Match: XP_023523962.1 (LOW QUALITY PROTEIN: P-loop NTPase domain-containing protein LPA1 homolog 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1187 bits (3070), Expect = 0.0
Identity = 610/701 (87.02%), Postives = 647/701 (92.30%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAE+ KLLY+VVVD G+K E  KGSFRYTRH LQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAEVAKLLYIVVVDEGEKLEKEKGSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGK--TEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFE 122
           E STD FLPE+ D+LG VSLVG+  +E D+++Y++C D  EIF NLGS EGDN KN PFE
Sbjct: 61  EASTDVFLPEESDILGLVSLVGQGQSEKDTDYYHNCFDGNEIFENLGSREGDNSKNIPFE 120

Query: 123 LYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGC 182
           LYK+SRTAVV+RETFLDVICDSLAKYKYVGPNQRADL+LACRIRERKESVTVLLCGTSGC
Sbjct: 121 LYKKSRTAVVKRETFLDVICDSLAKYKYVGPNQRADLILACRIRERKESVTVLLCGTSGC 180

Query: 183 GKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESK 242
           GKSTLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESK
Sbjct: 181 GKSTLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESK 240

Query: 243 ARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQS 302
           ARKKAKKLAGISHKH+KDE++DGL+T NSD+ LIGS KV NNLELISPKQMAIEGFKAQS
Sbjct: 241 ARKKAKKLAGISHKHMKDETSDGLITINSDNQLIGSAKVLNNLELISPKQMAIEGFKAQS 300

Query: 303 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERF 362
           EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERF
Sbjct: 301 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERF 360

Query: 363 AVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS 422
           AVRAKYM LDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS
Sbjct: 361 AVRAKYMTLDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS 420

Query: 423 CLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 482
           CLRRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG
Sbjct: 421 CLRRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 480

Query: 483 SVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG 542
           SVAKAWPVDSVDSNGKPLLGLK DD+ GN M GSL IGKVEPVNLQFGLYGISAWPSS G
Sbjct: 481 SVAKAWPVDSVDSNGKPLLGLKADDSTGNLMFGSLQIGKVEPVNLQFGLYGISAWPSSGG 540

Query: 543 PSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGS 602
           PSHA S+DESRAE TDTGSRYFSSCCSSPRILDGPSKELKED SVHGSD+EVDDP ESGS
Sbjct: 541 PSHAGSVDESRAEGTDTGSRYFSSCCSSPRILDGPSKELKEDISVHGSDEEVDDPPESGS 600

Query: 603 DEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDL 662
           DED SDDG KM HEEIGSVDEES+KSDEEYDDL LQDIL++GYQSDED+EE+K   E   
Sbjct: 601 DEDFSDDGGKMIHEEIGSVDEESSKSDEEYDDLALQDILDNGYQSDEDNEEIKKNCEDGF 660

Query: 663 MDQFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKN 701
             QFR KKG K++QNL+PF QSK+GTF+PL SY SMH EKN
Sbjct: 661 RYQFRHKKGLKYNQNLEPFQQSKKGTFEPLPSYXSMHTEKN 701

BLAST of MC03g0349 vs. NCBI nr
Match: XP_031736118.1 (P-loop NTPase domain-containing protein LPA1 homolog 1 [Cucumis sativus] >KGN64816.1 hypothetical protein Csa_022821 [Cucumis sativus])

HSP 1 Score: 1185 bits (3065), Expect = 0.0
Identity = 611/706 (86.54%), Postives = 647/706 (91.64%), Query Frame = 0

Query: 1   TAMAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELI 60
           T MAE+ KLLY+VVVD  + PE GK SFRYTRH LQSTLQLMGCKPRHAFKISKWVFELI
Sbjct: 32  TGMAEVAKLLYIVVVDEEENPEKGKRSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELI 91

Query: 61  RKEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFE 120
           RKE ST+ FLPE+ ++LGSVSL  KTE D N YY+C D +E FN     EGDN  N PFE
Sbjct: 92  RKEASTENFLPEERNILGSVSLGEKTETDGNRYYNCFDGKETFNQ-SPREGDNNNNIPFE 151

Query: 121 LYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGC 180
           LYKRSRTAVV RE FLDVICDSLAKYKYVGPNQRADL+LACRIRERKESVTVLLCGTSGC
Sbjct: 152 LYKRSRTAVVGRELFLDVICDSLAKYKYVGPNQRADLILACRIRERKESVTVLLCGTSGC 211

Query: 181 GKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESK 240
           GKSTLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESK
Sbjct: 212 GKSTLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESK 271

Query: 241 ARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQS 300
           ARKKAKKLAGISHKHLKDE+A+GL+++NSD+ LIG  KV NNLELISPKQMAIEGFKAQS
Sbjct: 272 ARKKAKKLAGISHKHLKDETANGLISRNSDNPLIGPAKVANNLELISPKQMAIEGFKAQS 331

Query: 301 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERF 360
           EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERF
Sbjct: 332 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERF 391

Query: 361 AVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS 420
           AVRAKYM LDPAKNKYVKYIRNIRTIQDYLC RADKHLVPKINNTNVDKSVAAIQATLFS
Sbjct: 392 AVRAKYMTLDPAKNKYVKYIRNIRTIQDYLCNRADKHLVPKINNTNVDKSVAAIQATLFS 451

Query: 421 CLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 480
           CLRRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG
Sbjct: 452 CLRRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 511

Query: 481 SVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG 540
           SVAKAWPVDSVDSNGKPLLGLK DD+V N M GSL IGKVEPVNLQFGLYGISAWPSSSG
Sbjct: 512 SVAKAWPVDSVDSNGKPLLGLKEDDSVENLMLGSLQIGKVEPVNLQFGLYGISAWPSSSG 571

Query: 541 PSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGS 600
           PSHA S+DESRAE TDTGSRYFSSCCSSPRI DGPSKELKEDNSVHGSD+EVDDP ESGS
Sbjct: 572 PSHAGSVDESRAEGTDTGSRYFSSCCSSPRIFDGPSKELKEDNSVHGSDEEVDDPPESGS 631

Query: 601 DEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDL 660
           DED SDDGDKMFHEEIGSVDEESTKSDEEYDDL +QDIL+SGYQSDEDD+ VK K E+  
Sbjct: 632 DEDFSDDGDKMFHEEIGSVDEESTKSDEEYDDLAMQDILDSGYQSDEDDK-VKTKIEHGF 691

Query: 661 MDQFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEK-NGAVC 705
           MD+FRL KG K+SQNL+PF Q+K+GTF+PLH + S+H +K NGAVC
Sbjct: 692 MDKFRLIKGLKYSQNLEPFQQNKKGTFEPLHPFPSIHTDKKNGAVC 735

BLAST of MC03g0349 vs. ExPASy TrEMBL
Match: A0A6J1DHF5 (P-loop NTPase domain-containing protein LPA1 homolog 1-like OS=Momordica charantia OX=3673 GN=LOC111020498 PE=4 SV=1)

HSP 1 Score: 1392 bits (3603), Expect = 0.0
Identity = 703/703 (100.00%), Postives = 703/703 (100.00%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 122
           EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY
Sbjct: 61  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 120

Query: 123 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 182
           KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK
Sbjct: 121 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 180

Query: 183 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 242
           STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR
Sbjct: 181 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 240

Query: 243 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 302
           KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM
Sbjct: 241 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 300

Query: 303 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 362
           VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV
Sbjct: 301 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 360

Query: 363 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 422
           RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL
Sbjct: 361 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 420

Query: 423 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 482
           RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV
Sbjct: 421 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 480

Query: 483 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 542
           AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS
Sbjct: 481 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 540

Query: 543 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 602
           HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE
Sbjct: 541 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 600

Query: 603 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 662
           DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD
Sbjct: 601 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 660

Query: 663 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC 705
           QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC
Sbjct: 661 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEKNGAVC 703

BLAST of MC03g0349 vs. ExPASy TrEMBL
Match: A0A5A7UE49 (P-loop NTPase domain-containing protein LPA1-like protein 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold60G001690 PE=4 SV=1)

HSP 1 Score: 1189 bits (3077), Expect = 0.0
Identity = 612/704 (86.93%), Postives = 652/704 (92.61%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAE+ KLLY+VVVD  +KPE  K SFRYTRH LQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAEVAKLLYIVVVDEEEKPEKEKTSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFELY 122
           E ST+ FLPE+ ++LGS+SL G TE D N YY+CLD +E FN  GS EGDN  N PFELY
Sbjct: 61  EASTENFLPEEWNVLGSISLGGNTESDGNQYYNCLDGKETFNQ-GSREGDNSNNIPFELY 120

Query: 123 KRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCGK 182
           KRSRTAVV RE+FLDVICDSLAKYKYVGPNQRADL+LACRIRERKESVTVLLCGTSGCGK
Sbjct: 121 KRSRTAVVGRESFLDVICDSLAKYKYVGPNQRADLILACRIRERKESVTVLLCGTSGCGK 180

Query: 183 STLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKAR 242
           STLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESKAR
Sbjct: 181 STLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESKAR 240

Query: 243 KKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSEM 302
           KKAKKLAGISHKHLKDE+A+GL+++NSD+ L GS KV NNLELISPKQMAIEGFKAQSEM
Sbjct: 241 KKAKKLAGISHKHLKDETANGLISRNSDNPLTGSAKVTNNLELISPKQMAIEGFKAQSEM 300

Query: 303 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFAV 362
           VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERFAV
Sbjct: 301 VIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERFAV 360

Query: 363 RAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSCL 422
           RAKYM LDPAKN+YVKYIRNIRTIQDYLC RADKHLVPKINNTNVDKSVAAIQATLFSCL
Sbjct: 361 RAKYMTLDPAKNRYVKYIRNIRTIQDYLCNRADKHLVPKINNTNVDKSVAAIQATLFSCL 420

Query: 423 RRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 482
           RRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV
Sbjct: 421 RRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDGSV 480

Query: 483 AKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSGPS 542
           AKAWPVDSVD NGKPLLGLK DD+VGN M GSL IGKVEPVNLQFGLYGISAWPSSSGPS
Sbjct: 481 AKAWPVDSVDRNGKPLLGLKEDDSVGNLMLGSLQIGKVEPVNLQFGLYGISAWPSSSGPS 540

Query: 543 HASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGSDE 602
           HA S+DESRAE TDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSD+EVDDP ESGSDE
Sbjct: 541 HAGSVDESRAEGTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDEEVDDPPESGSDE 600

Query: 603 DISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDLMD 662
           D SDDG+K+FHEEIGSVDEESTKSDEEYDDL +QDIL+SGYQSDEDD+ VK K E+D MD
Sbjct: 601 DFSDDGNKIFHEEIGSVDEESTKSDEEYDDLAMQDILDSGYQSDEDDK-VKTKVEHDFMD 660

Query: 663 QFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEK-NGAVC 705
           +FRL KG K+SQ+L+PF Q+K+GTF+PLHSY S+H EK N AVC
Sbjct: 661 KFRLIKGLKYSQSLEPFQQNKKGTFEPLHSYPSIHTEKKNEAVC 702

BLAST of MC03g0349 vs. ExPASy TrEMBL
Match: A0A0A0LXV3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G111370 PE=4 SV=1)

HSP 1 Score: 1185 bits (3065), Expect = 0.0
Identity = 611/706 (86.54%), Postives = 647/706 (91.64%), Query Frame = 0

Query: 1   TAMAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELI 60
           T MAE+ KLLY+VVVD  + PE GK SFRYTRH LQSTLQLMGCKPRHAFKISKWVFELI
Sbjct: 32  TGMAEVAKLLYIVVVDEEENPEKGKRSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELI 91

Query: 61  RKEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFE 120
           RKE ST+ FLPE+ ++LGSVSL  KTE D N YY+C D +E FN     EGDN  N PFE
Sbjct: 92  RKEASTENFLPEERNILGSVSLGEKTETDGNRYYNCFDGKETFNQ-SPREGDNNNNIPFE 151

Query: 121 LYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGC 180
           LYKRSRTAVV RE FLDVICDSLAKYKYVGPNQRADL+LACRIRERKESVTVLLCGTSGC
Sbjct: 152 LYKRSRTAVVGRELFLDVICDSLAKYKYVGPNQRADLILACRIRERKESVTVLLCGTSGC 211

Query: 181 GKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESK 240
           GKSTLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESK
Sbjct: 212 GKSTLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESK 271

Query: 241 ARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQS 300
           ARKKAKKLAGISHKHLKDE+A+GL+++NSD+ LIG  KV NNLELISPKQMAIEGFKAQS
Sbjct: 272 ARKKAKKLAGISHKHLKDETANGLISRNSDNPLIGPAKVANNLELISPKQMAIEGFKAQS 331

Query: 301 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERF 360
           EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERF
Sbjct: 332 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERF 391

Query: 361 AVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS 420
           AVRAKYM LDPAKNKYVKYIRNIRTIQDYLC RADKHLVPKINNTNVDKSVAAIQATLFS
Sbjct: 392 AVRAKYMTLDPAKNKYVKYIRNIRTIQDYLCNRADKHLVPKINNTNVDKSVAAIQATLFS 451

Query: 421 CLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 480
           CLRRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG
Sbjct: 452 CLRRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 511

Query: 481 SVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG 540
           SVAKAWPVDSVDSNGKPLLGLK DD+V N M GSL IGKVEPVNLQFGLYGISAWPSSSG
Sbjct: 512 SVAKAWPVDSVDSNGKPLLGLKEDDSVENLMLGSLQIGKVEPVNLQFGLYGISAWPSSSG 571

Query: 541 PSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGS 600
           PSHA S+DESRAE TDTGSRYFSSCCSSPRI DGPSKELKEDNSVHGSD+EVDDP ESGS
Sbjct: 572 PSHAGSVDESRAEGTDTGSRYFSSCCSSPRIFDGPSKELKEDNSVHGSDEEVDDPPESGS 631

Query: 601 DEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDL 660
           DED SDDGDKMFHEEIGSVDEESTKSDEEYDDL +QDIL+SGYQSDEDD+ VK K E+  
Sbjct: 632 DEDFSDDGDKMFHEEIGSVDEESTKSDEEYDDLAMQDILDSGYQSDEDDK-VKTKIEHGF 691

Query: 661 MDQFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEK-NGAVC 705
           MD+FRL KG K+SQNL+PF Q+K+GTF+PLH + S+H +K NGAVC
Sbjct: 692 MDKFRLIKGLKYSQNLEPFQQNKKGTFEPLHPFPSIHTDKKNGAVC 735

BLAST of MC03g0349 vs. ExPASy TrEMBL
Match: A0A6J1GAC9 (P-loop NTPase domain-containing protein LPA1 homolog 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452390 PE=4 SV=1)

HSP 1 Score: 1173 bits (3034), Expect = 0.0
Identity = 603/702 (85.90%), Postives = 644/702 (91.74%), Query Frame = 0

Query: 1   TAMAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELI 60
           T MAE+ KLLY+VVVD G+K E  KGSFRYTRH LQSTLQLMGCKPRHAFKISKWVFELI
Sbjct: 8   TGMAEVAKLLYIVVVDEGEKLEKEKGSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELI 67

Query: 61  RKEDSTDAFLPEDLDMLGSVSLVGK--TEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNP 120
           RKE STD FLPE+ D+LG VSLVG+  +E D+++Y++C D  EIF NLGS EGDN KN P
Sbjct: 68  RKEASTDVFLPEESDILGLVSLVGQGQSEKDTDYYHNCFDGNEIFENLGSREGDNSKNIP 127

Query: 121 FELYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTS 180
           FELYK+SRTAVV+RETFLDVICDSLA YKYVGPNQRADL+LACRIRERKESVTVLLCGTS
Sbjct: 128 FELYKKSRTAVVKRETFLDVICDSLATYKYVGPNQRADLILACRIRERKESVTVLLCGTS 187

Query: 181 GCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAE 240
           GCGKSTLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAE
Sbjct: 188 GCGKSTLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAE 247

Query: 241 SKARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKA 300
           SKARKKAKKLAGISHKH+KDE++DGL+T NSD+ LIGS KV NNLELISPKQMAIEGFKA
Sbjct: 248 SKARKKAKKLAGISHKHMKDETSDGLITINSDNQLIGSAKVLNNLELISPKQMAIEGFKA 307

Query: 301 QSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLE 360
           QSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLE
Sbjct: 308 QSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLE 367

Query: 361 RFAVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATL 420
           RFAVRAKYM LDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATL
Sbjct: 368 RFAVRAKYMTLDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATL 427

Query: 421 FSCLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNT 480
           FSCLRRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNT
Sbjct: 428 FSCLRRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNT 487

Query: 481 DGSVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSS 540
           DGSVAKAWPVDSVDSNGKPLLGLK DD+ GN + GSL IGKVEPVNLQFGLYGISAWPSS
Sbjct: 488 DGSVAKAWPVDSVDSNGKPLLGLKADDSTGNLIFGSLQIGKVEPVNLQFGLYGISAWPSS 547

Query: 541 SGPSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHES 600
            GPSHA S+DE RAE TDTGSRYFSSCCSSPRILDGPSKELKED SVHGSD+EVDDP ES
Sbjct: 548 GGPSHAGSVDELRAEGTDTGSRYFSSCCSSPRILDGPSKELKEDISVHGSDEEVDDPPES 607

Query: 601 GSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEY 660
           GSDED SDDG KM HEEIGSVDEES+KSDEEYDDL LQDIL++GYQSDED+EE+K   E 
Sbjct: 608 GSDEDFSDDGGKMIHEEIGSVDEESSKSDEEYDDLALQDILDNGYQSDEDNEEIKKNCED 667

Query: 661 DLMDQFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEK 700
           D   QFR+KKG K++QNL+P  QSK+GTF+PL SY   + E+
Sbjct: 668 DFRYQFRVKKGLKYNQNLEPLQQSKKGTFEPLPSYIYAYREE 709

BLAST of MC03g0349 vs. ExPASy TrEMBL
Match: A0A6J1GAE4 (P-loop NTPase domain-containing protein LPA1 homolog 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452390 PE=4 SV=1)

HSP 1 Score: 1171 bits (3029), Expect = 0.0
Identity = 602/700 (86.00%), Postives = 643/700 (91.86%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPENGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIRK 62
           MAE+ KLLY+VVVD G+K E  KGSFRYTRH LQSTLQLMGCKPRHAFKISKWVFELIRK
Sbjct: 1   MAEVAKLLYIVVVDEGEKLEKEKGSFRYTRHVLQSTLQLMGCKPRHAFKISKWVFELIRK 60

Query: 63  EDSTDAFLPEDLDMLGSVSLVGK--TEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFE 122
           E STD FLPE+ D+LG VSLVG+  +E D+++Y++C D  EIF NLGS EGDN KN PFE
Sbjct: 61  EASTDVFLPEESDILGLVSLVGQGQSEKDTDYYHNCFDGNEIFENLGSREGDNSKNIPFE 120

Query: 123 LYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGC 182
           LYK+SRTAVV+RETFLDVICDSLA YKYVGPNQRADL+LACRIRERKESVTVLLCGTSGC
Sbjct: 121 LYKKSRTAVVKRETFLDVICDSLATYKYVGPNQRADLILACRIRERKESVTVLLCGTSGC 180

Query: 183 GKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESK 242
           GKSTLSALLGSRLGITTV+STDSIRHMMRSFVD+KQNPLLW+STYHAGEFLDPVAVAESK
Sbjct: 181 GKSTLSALLGSRLGITTVISTDSIRHMMRSFVDEKQNPLLWSSTYHAGEFLDPVAVAESK 240

Query: 243 ARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQS 302
           ARKKAKKLAGISHKH+KDE++DGL+T NSD+ LIGS KV NNLELISPKQMAIEGFKAQS
Sbjct: 241 ARKKAKKLAGISHKHMKDETSDGLITINSDNQLIGSAKVLNNLELISPKQMAIEGFKAQS 300

Query: 303 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERF 362
           EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYI+NEEKHLERF
Sbjct: 301 EMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIVNEEKHLERF 360

Query: 363 AVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS 422
           AVRAKYM LDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS
Sbjct: 361 AVRAKYMTLDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFS 420

Query: 423 CLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 482
           CLRRREAGEQLYD SRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG
Sbjct: 421 CLRRREAGEQLYDSSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKGSKHLMALVNTDG 480

Query: 483 SVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG 542
           SVAKAWPVDSVDSNGKPLLGLK DD+ GN + GSL IGKVEPVNLQFGLYGISAWPSS G
Sbjct: 481 SVAKAWPVDSVDSNGKPLLGLKADDSTGNLIFGSLQIGKVEPVNLQFGLYGISAWPSSGG 540

Query: 543 PSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDEVDDPHESGS 602
           PSHA S+DE RAE TDTGSRYFSSCCSSPRILDGPSKELKED SVHGSD+EVDDP ESGS
Sbjct: 541 PSHAGSVDELRAEGTDTGSRYFSSCCSSPRILDGPSKELKEDISVHGSDEEVDDPPESGS 600

Query: 603 DEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKFEYDL 662
           DED SDDG KM HEEIGSVDEES+KSDEEYDDL LQDIL++GYQSDED+EE+K   E D 
Sbjct: 601 DEDFSDDGGKMIHEEIGSVDEESSKSDEEYDDLALQDILDNGYQSDEDNEEIKKNCEDDF 660

Query: 663 MDQFRLKKGFKHSQNLDPFPQSKRGTFDPLHSYSSMHAEK 700
             QFR+KKG K++QNL+P  QSK+GTF+PL SY   + E+
Sbjct: 661 RYQFRVKKGLKYNQNLEPLQQSKKGTFEPLPSYIYAYREE 700

BLAST of MC03g0349 vs. TAIR 10
Match: AT5G60760.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 800.4 bits (2066), Expect = 1.1e-231
Identity = 454/718 (63.23%), Postives = 537/718 (74.79%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPE--------NGKGSFRYTRHGLQSTLQLMGCKPRHAFKISK 62
           M E  K++Y+VVVD  D  E          K SFRYTR  LQSTLQLMGCK RHAFKIS+
Sbjct: 1   MTETTKVMYIVVVDGTDTAETVEEDGTGTWKDSFRYTRPVLQSTLQLMGCKARHAFKISR 60

Query: 63  WVFELIRKEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYD---CLDEEEI--FNNLGSG 122
            VFELIR E S        L +  S+S     E +     D   C + E+    N+L + 
Sbjct: 61  RVFELIRSEGS--------LILSPSLSPSHSKESEFQKTGDASACANVEKANKVNSLATD 120

Query: 123 EGDNCKNNPFELYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKES 182
           + D  K+ PFE+YKR  T VV RE F++V+CD+LA+YKYVG +QRADL+L+CRIRERKES
Sbjct: 121 DVDKSKSKPFEVYKRRTTVVVSREIFVNVVCDALAEYKYVGHDQRADLILSCRIRERKES 180

Query: 183 VTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGE 242
           VTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVD+KQNPLLWASTYHAGE
Sbjct: 181 VTVLLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDEKQNPLLWASTYHAGE 240

Query: 243 FLDPVAVAESKA-RKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISP 302
           +LDPVAVAESKA R++AKK+  I  +  K ++++G   KN+    +GSTK  N   L+SP
Sbjct: 241 YLDPVAVAESKARRRRAKKMDSIEDE--KAKASEGGKAKNTQQTDVGSTK--NTPVLLSP 300

Query: 303 KQMAIEGFKAQSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMI 362
           KQMA+EGFKAQSEMVID+LDRLITAWEERKESVIVEGVHLSL+FVMGLMKKHPSI+PFM+
Sbjct: 301 KQMAVEGFKAQSEMVIDNLDRLITAWEERKESVIVEGVHLSLNFVMGLMKKHPSIVPFMV 360

Query: 363 YIMNEEKHLERFAVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVD 422
           YI NEEKHLERFAVRAKYM LDP KNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVD
Sbjct: 361 YIANEEKHLERFAVRAKYMTLDPEKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVD 420

Query: 423 KSVAAIQATLFSCLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG 482
           KSVAAI AT+FSCLRRREAGE LYD + NTVSV+D+EYRNQC A SLSSKGMFQ+IQRKG
Sbjct: 421 KSVAAIHATVFSCLRRREAGEHLYDATTNTVSVIDDEYRNQCTANSLSSKGMFQLIQRKG 480

Query: 483 -SKHLMALVNTDGSVAKAWPV-DSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQ 542
            S+HLMAL+NTDG+ A+ WPV   VD +GKP+   +  +  G        + K EPVNLQ
Sbjct: 481 SSRHLMALLNTDGTFARTWPVTGKVDESGKPVFCNEMIEENGMEHPVYGYLQKAEPVNLQ 540

Query: 543 FGLYGISAWPSSSGPSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVH 602
           FGL+GISAWPS    S A S+D+ +A+  +T SRY+SSCCSSPR+ +G SKELKED SVH
Sbjct: 541 FGLFGISAWPSDGATSRAGSVDDCKADMAETSSRYYSSCCSSPRMSEGTSKELKEDQSVH 600

Query: 603 GSDDEV-DDPHESGSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQS 662
           GSD+EV DDP E   D D SDD +K  H+E+GSVDE+STKSDEEYDDL ++D     Y +
Sbjct: 601 GSDEEVEDDPPE--PDTDFSDDDNKRDHDEVGSVDEQSTKSDEEYDDLAMED---KSYWT 660

Query: 663 DEDDEEVKGKFEYDLMDQFRLKKGFKHSQNLDPFPQ-SKRGTFDPLHSYSSMHAEKNG 703
           DE++E           +  +  K  K+ QNLD F + + +   +PL   +S+   +NG
Sbjct: 661 DEEEESRDTIAIMSEKNNKQATKEDKYIQNLDLFLRTANQQLVEPLQLCASLLTCENG 701

BLAST of MC03g0349 vs. TAIR 10
Match: AT3G45090.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 788.1 bits (2034), Expect = 5.8e-228
Identity = 435/687 (63.32%), Postives = 513/687 (74.67%), Query Frame = 0

Query: 3   MAELPKLLYVVVVDAGDKPE-NGKGSFRYTRHGLQSTLQLMGCKPRHAFKISKWVFELIR 62
           M E  K+LY+VV + GD  + NG  SFRYTR  LQSTLQLMGCK RHAFKIS+ VFELIR
Sbjct: 2   MTEATKVLYIVVREEGDDDDNNGDDSFRYTRPVLQSTLQLMGCKARHAFKISRRVFELIR 61

Query: 63  KEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGDNCKNNPFEL 122
            E S +       +   +  + G T ++     +CL        + +G+ D  K+ PFE+
Sbjct: 62  SEGSCNTSPENGKEPEFAKEVGGSTCVEK---LNCL--------VVAGDVDKNKSKPFEM 121

Query: 123 YKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTVLLCGTSGCG 182
           YKR  T VV RE F+DV+CD+LA+YKYVG +QRADL+LACRIRERKESVTVLLCGTSGCG
Sbjct: 122 YKRRTTVVVSREIFVDVVCDALAEYKYVGRDQRADLILACRIRERKESVTVLLCGTSGCG 181

Query: 183 KSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLDPVAVAESKA 242
           KSTLSALLGSRLGITTVVSTDSIRHMMRSF D+KQNPLLWASTYHAGE+LDPVAVAESKA
Sbjct: 182 KSTLSALLGSRLGITTVVSTDSIRHMMRSFADEKQNPLLWASTYHAGEYLDPVAVAESKA 241

Query: 243 RKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMAIEGFKAQSE 302
           ++KAKKL G    +   +  D            GS    +  EL+S KQMAIEG+KAQSE
Sbjct: 242 KRKAKKLKGSRGVNSNAQKTDA-----------GSNS--STTELLSHKQMAIEGYKAQSE 301

Query: 303 MVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMNEEKHLERFA 362
           MVIDSLDRLIT WEER ESV+VEGVHLSL+FVMGLMKKHPSI+PFM+YI NEEKHLERFA
Sbjct: 302 MVIDSLDRLITTWEERNESVVVEGVHLSLNFVMGLMKKHPSIVPFMVYIANEEKHLERFA 361

Query: 363 VRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVAAIQATLFSC 422
           VRAKYM LDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVA I AT+F C
Sbjct: 362 VRAKYMTLDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVATIHATVFGC 421

Query: 423 LRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG-SKHLMALVNTDG 482
           LRRRE GE+LYD + NTVSV+D+E+RNQC A SL+SKGMFQ+IQR+G S+  MAL NTDG
Sbjct: 422 LRRRETGEKLYDTTTNTVSVIDDEHRNQCAANSLTSKGMFQVIQRQGSSRRFMALCNTDG 481

Query: 483 SVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYGISAWPSSSG 542
           +VAK WPV SV    KP++  + DD   +       + K EPVNLQFG +GISAWPS   
Sbjct: 482 TVAKTWPVASVGKIRKPVVNTEMDDGTEH------QLHKAEPVNLQFGHFGISAWPSDGA 541

Query: 543 PSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDE----VDDPH 602
            SHA S+D+ RA+  +TGSR++SSCCSSPR  DGPSKEL E+ SV+GSD++     DD H
Sbjct: 542 TSHAGSVDDLRADIIETGSRHYSSCCSSPRTSDGPSKELMEEQSVNGSDEDDEEGDDDFH 601

Query: 603 ESGSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDEDDEEVKGKF 662
           E  SDED+SD+ D+   +EIGSVDEESTKSDEEYDDL ++D     Y +D ++EE +   
Sbjct: 602 EPDSDEDLSDNNDERNRDEIGSVDEESTKSDEEYDDLAMED---KSYWTDNEEEESRDTI 655

Query: 663 ----EYDLMDQFRLKKGFKHSQNLDPF 680
               + +  +  +  K  K+SQNLD F
Sbjct: 662 SMVSQNNHNEASKTNKDDKYSQNLDLF 655

BLAST of MC03g0349 vs. TAIR 10
Match: AT3G45090.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 724.9 bits (1870), Expect = 6.1e-209
Identity = 401/636 (63.05%), Postives = 475/636 (74.69%), Query Frame = 0

Query: 53  SKWVFELIRKEDSTDAFLPEDLDMLGSVSLVGKTEIDSNHYYDCLDEEEIFNNLGSGEGD 112
           S+ VFELIR E S +       +   +  + G T ++     +CL        + +G+ D
Sbjct: 34  SRRVFELIRSEGSCNTSPENGKEPEFAKEVGGSTCVEK---LNCL--------VVAGDVD 93

Query: 113 NCKNNPFELYKRSRTAVVRRETFLDVICDSLAKYKYVGPNQRADLMLACRIRERKESVTV 172
             K+ PFE+YKR  T VV RE F+DV+CD+LA+YKYVG +QRADL+LACRIRERKESVTV
Sbjct: 94  KNKSKPFEMYKRRTTVVVSREIFVDVVCDALAEYKYVGRDQRADLILACRIRERKESVTV 153

Query: 173 LLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFVDDKQNPLLWASTYHAGEFLD 232
           LLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSF D+KQNPLLWASTYHAGE+LD
Sbjct: 154 LLCGTSGCGKSTLSALLGSRLGITTVVSTDSIRHMMRSFADEKQNPLLWASTYHAGEYLD 213

Query: 233 PVAVAESKARKKAKKLAGISHKHLKDESADGLMTKNSDSLLIGSTKVPNNLELISPKQMA 292
           PVAVAESKA++KAKKL G    +   +  D            GS    +  EL+S KQMA
Sbjct: 214 PVAVAESKAKRKAKKLKGSRGVNSNAQKTDA-----------GSNS--STTELLSHKQMA 273

Query: 293 IEGFKAQSEMVIDSLDRLITAWEERKESVIVEGVHLSLSFVMGLMKKHPSIIPFMIYIMN 352
           IEG+KAQSEMVIDSLDRLIT WEER ESV+VEGVHLSL+FVMGLMKKHPSI+PFM+YI N
Sbjct: 274 IEGYKAQSEMVIDSLDRLITTWEERNESVVVEGVHLSLNFVMGLMKKHPSIVPFMVYIAN 333

Query: 353 EEKHLERFAVRAKYMALDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVA 412
           EEKHLERFAVRAKYM LDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVA
Sbjct: 334 EEKHLERFAVRAKYMTLDPAKNKYVKYIRNIRTIQDYLCKRADKHLVPKINNTNVDKSVA 393

Query: 413 AIQATLFSCLRRREAGEQLYDPSRNTVSVVDEEYRNQCVAYSLSSKGMFQMIQRKG-SKH 472
            I AT+F CLRRRE GE+LYD + NTVSV+D+E+RNQC A SL+SKGMFQ+IQR+G S+ 
Sbjct: 394 TIHATVFGCLRRRETGEKLYDTTTNTVSVIDDEHRNQCAANSLTSKGMFQVIQRQGSSRR 453

Query: 473 LMALVNTDGSVAKAWPVDSVDSNGKPLLGLKTDDNVGNWMRGSLNIGKVEPVNLQFGLYG 532
            MAL NTDG+VAK WPV SV    KP++  + DD   +       + K EPVNLQFG +G
Sbjct: 454 FMALCNTDGTVAKTWPVASVGKIRKPVVNTEMDDGTEH------QLHKAEPVNLQFGHFG 513

Query: 533 ISAWPSSSGPSHASSLDESRAEWTDTGSRYFSSCCSSPRILDGPSKELKEDNSVHGSDDE 592
           ISAWPS    SHA S+D+ RA+  +TGSR++SSCCSSPR  DGPSKEL E+ SV+GSD++
Sbjct: 514 ISAWPSDGATSHAGSVDDLRADIIETGSRHYSSCCSSPRTSDGPSKELMEEQSVNGSDED 573

Query: 593 ----VDDPHESGSDEDISDDGDKMFHEEIGSVDEESTKSDEEYDDLELQDILESGYQSDE 652
                DD HE  SDED+SD+ D+   +EIGSVDEESTKSDEEYDDL ++D     Y +D 
Sbjct: 574 DEEGDDDFHEPDSDEDLSDNNDERNRDEIGSVDEESTKSDEEYDDLAMED---KSYWTDN 633

Query: 653 DDEEVKGKF----EYDLMDQFRLKKGFKHSQNLDPF 680
           ++EE +       + +  +  +  K  K+SQNLD F
Sbjct: 634 EEEESRDTISMVSQNNHNEASKTNKDDKYSQNLDLF 636

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJH91.6e-23063.23P-loop NTPase domain-containing protein LPA1 homolog 1 OS=Arabidopsis thaliana O... [more]
Q93ZS18.2e-22763.32P-loop NTPase domain-containing protein LPA1 homolog 2 OS=Arabidopsis thaliana O... [more]
B9F4I82.5e-21564.24P-loop NTPase domain-containing protein LPA1 OS=Oryza sativa subsp. japonica OX=... [more]
Q651A12.7e-19358.62P-loop NTPase domain-containing protein LPA1 homolog OS=Oryza sativa subsp. japo... [more]
Q8T1P11.6e-7635.11Uncharacterized protein DDB_G0273453/DDB_G0273565 OS=Dictyostelium discoideum OX... [more]
Match NameE-valueIdentityDescription
XP_022152872.10.0100.00P-loop NTPase domain-containing protein LPA1 homolog 1-like [Momordica charantia... [more]
XP_038896070.10.088.76P-loop NTPase domain-containing protein LPA1 homolog 1-like [Benincasa hispida][more]
KAA0051769.10.086.93P-loop NTPase domain-containing protein LPA1-like protein 1-like [Cucumis melo v... [more]
XP_023523962.10.087.02LOW QUALITY PROTEIN: P-loop NTPase domain-containing protein LPA1 homolog 1-like... [more]
XP_031736118.10.086.54P-loop NTPase domain-containing protein LPA1 homolog 1 [Cucumis sativus] >KGN648... [more]
Match NameE-valueIdentityDescription
A0A6J1DHF50.0100.00P-loop NTPase domain-containing protein LPA1 homolog 1-like OS=Momordica charant... [more]
A0A5A7UE490.086.93P-loop NTPase domain-containing protein LPA1-like protein 1-like OS=Cucumis melo... [more]
A0A0A0LXV30.086.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G111370 PE=4 SV=1[more]
A0A6J1GAC90.085.90P-loop NTPase domain-containing protein LPA1 homolog 1-like isoform X1 OS=Cucurb... [more]
A0A6J1GAE40.086.00P-loop NTPase domain-containing protein LPA1 homolog 1-like isoform X2 OS=Cucurb... [more]
Match NameE-valueIdentityDescription
AT5G60760.11.1e-23163.23P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G45090.15.8e-22863.32P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G45090.26.1e-20963.05P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 166..425
e-value: 8.8E-10
score: 40.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 171..424
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 672..693
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 574..593
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 572..609
NoneNo IPR availablePANTHERPTHR33477P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1coord: 5..701
NoneNo IPR availablePANTHERPTHR33477:SF3P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1coord: 5..701

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0349.1MC03g0349.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane