MC03g0332 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0332
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	High-affinity nitrate transporter 2.2
Location	MC03: 9732734 .. 9734401 (+)
RNA-Seq Expression	MC03g0332
Synteny	MC03g0332

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGAATGTTGAGGGATCCCCAGGGAGCTCGATGCATGGAGTGACCGGGAGAGAGCAGACATTCGCGTTCTCGGTGGCGTCCCCAATAGTCCCAACAGACACGAGCGCCAAATTCGCACTCCCGGTGGACTCGGAGCACAAGGCGAAGGTGTTCAAGATCTTCTCCTTAGCCAACCCCCACATGAGAACCTTCCACCTCTCATGGATCTCCTTCTTCACCTGCTTCGTCTCCACCTTCGCCGCCGCCCCTCTCGTCCCCATCATCCGCGACAACCTCAACCTCACCAAGTCCGACATCGGCAACGCCGGCGTCACCTCCGTCTCCGGCAGCATCTTCTCCCGTCTCGTCATGGGCGCCGTCTGCGACCTCCTCGGCCCCCGCTACGGCTGCGCCTTCCTCATCATGCTCTCCGCCCCCACCGTCTTCTGCATGTCCTTCGTCTCCTCCCCCGCCGGCTACATCGCCGTCCGCTTCATGATCGGCTTCTCCCTCGCCACCTTCGTCTCCTGCCAGTACTGGATGAGCACCATGTTCAACAGCCAGATCATCGGCCTCGTCAACGGCACCGCCGCCGGCTGGGGCAACATGGGCGGAGGCGCCACCCAGCTCCTCATGCCTCTCCTCTACGAGATCATCAAGAAGGTCGGGGCCACCCCCTTCACCGCCTGGAGGATCGCCTTCTTCATCCCCGGCTCCCTCCACGTCCTCATGGGCATTTTGGTCATGACTCTCGGCCAGGACTTGCCCGACGGAAATCTCAGCAGTCTCCAAAAGAAGGGCACCGTGGCCAAGGATAAATTTTCCAACGTAACTTCCCACCTTACCCTCTAGTAGATAGTGATGTCTATTATAATAATTAACGGAAGATGTTATTGTTAAATGTTTCAGGTGCTGTGGTACGCGGTCACCAACTACAGAACTTGGATCTTCGTTCTCCTGTATGGCTACTCAATGGGCGTCGAGCTCTCCACCGACAACGTCGTCGCCGAGTACTTCTACGACAGGTTCGACCTGAAGCTGCACACGGCGGGAATCATCGCAGCCACCTTCGGGATGGCGAATCTAGTGGCTCGCCCCTTCGGAGGGTACGCTTCCGACTTCGCAGCTCGATATTTCGGGATGAGGGGAAGGCTGTGGACCCTGTGGATCCTCCAAACGTGCGGTGGAGTGTTCTGCATGTGGCTTGGGAAGGCAACCCAGCTTCCAGTGGCCATAGTGGCCATGATCCTGTTCTCAATAGGAGCCCAGGCTGCGTGCGGAGCCACGTTTGGGATCATTCCCTTCATTTCCCGGCGCTCGTTGGGCATTATATCGGGCCTCACAGGCGCAGGAGGCAACTTCGGGTCTGGGTTGACTCAATTGGTGTTCTTCTCGACAACGAAATACTCCACAGACAAAGGGTTGTTCTTGATGGGCATTATGATCGTGTGCTGCACTCTTCCGGTCACATTGGTTCACTTCCCGCAGTGGGGGAGCATGTTCTTGCCGCCCACCAAACACGTGGAGAAATCCACAGAAGAATTCTATTATGGGTCTGAATGGACTGAGGACGAGAAGAAGAAGGGTCTGCACCAGCAGAGCATCAAGTTCGCTGAGAATAGCAGGTCCGAACGTGGCAGGAAAATTGCGTCAGCTCCAACCCCACCCAACGCCACTCCTTCCCATGTT

mRNA sequence

GGGAATGTTGAGGGATCCCCAGGGAGCTCGATGCATGGAGTGACCGGGAGAGAGCAGACATTCGCGTTCTCGGTGGCGTCCCCAATAGTCCCAACAGACACGAGCGCCAAATTCGCACTCCCGGTGGACTCGGAGCACAAGGCGAAGGTGTTCAAGATCTTCTCCTTAGCCAACCCCCACATGAGAACCTTCCACCTCTCATGGATCTCCTTCTTCACCTGCTTCGTCTCCACCTTCGCCGCCGCCCCTCTCGTCCCCATCATCCGCGACAACCTCAACCTCACCAAGTCCGACATCGGCAACGCCGGCGTCACCTCCGTCTCCGGCAGCATCTTCTCCCGTCTCGTCATGGGCGCCGTCTGCGACCTCCTCGGCCCCCGCTACGGCTGCGCCTTCCTCATCATGCTCTCCGCCCCCACCGTCTTCTGCATGTCCTTCGTCTCCTCCCCCGCCGGCTACATCGCCGTCCGCTTCATGATCGGCTTCTCCCTCGCCACCTTCGTCTCCTGCCAGTACTGGATGAGCACCATGTTCAACAGCCAGATCATCGGCCTCGTCAACGGCACCGCCGCCGGCTGGGGCAACATGGGCGGAGGCGCCACCCAGCTCCTCATGCCTCTCCTCTACGAGATCATCAAGAAGGTCGGGGCCACCCCCTTCACCGCCTGGAGGATCGCCTTCTTCATCCCCGGCTCCCTCCACGTCCTCATGGGCATTTTGGTCATGACTCTCGGCCAGGACTTGCCCGACGGAAATCTCAGCAGTCTCCAAAAGAAGGGCACCGTGGCCAAGGATAAATTTTCCAACGTGCTGTGGTACGCGGTCACCAACTACAGAACTTGGATCTTCGTTCTCCTGTATGGCTACTCAATGGGCGTCGAGCTCTCCACCGACAACGTCGTCGCCGAGTACTTCTACGACAGGTTCGACCTGAAGCTGCACACGGCGGGAATCATCGCAGCCACCTTCGGGATGGCGAATCTAGTGGCTCGCCCCTTCGGAGGGTACGCTTCCGACTTCGCAGCTCGATATTTCGGGATGAGGGGAAGGCTGTGGACCCTGTGGATCCTCCAAACGTGCGGTGGAGTGTTCTGCATGTGGCTTGGGAAGGCAACCCAGCTTCCAGTGGCCATAGTGGCCATGATCCTGTTCTCAATAGGAGCCCAGGCTGCGTGCGGAGCCACGTTTGGGATCATTCCCTTCATTTCCCGGCGCTCGTTGGGCATTATATCGGGCCTCACAGGCGCAGGAGGCAACTTCGGGTCTGGGTTGACTCAATTGGTGTTCTTCTCGACAACGAAATACTCCACAGACAAAGGGTTGTTCTTGATGGGCATTATGATCGTGTGCTGCACTCTTCCGGTCACATTGGTTCACTTCCCGCAGTGGGGGAGCATGTTCTTGCCGCCCACCAAACACGTGGAGAAATCCACAGAAGAATTCTATTATGGGTCTGAATGGACTGAGGACGAGAAGAAGAAGGGTCTGCACCAGCAGAGCATCAAGTTCGCTGAGAATAGCAGGTCCGAACGTGGCAGGAAAATTGCGTCAGCTCCAACCCCACCCAACGCCACTCCTTCCCATGTT

Coding sequence (CDS)

GGGAATGTTGAGGGATCCCCAGGGAGCTCGATGCATGGAGTGACCGGGAGAGAGCAGACATTCGCGTTCTCGGTGGCGTCCCCAATAGTCCCAACAGACACGAGCGCCAAATTCGCACTCCCGGTGGACTCGGAGCACAAGGCGAAGGTGTTCAAGATCTTCTCCTTAGCCAACCCCCACATGAGAACCTTCCACCTCTCATGGATCTCCTTCTTCACCTGCTTCGTCTCCACCTTCGCCGCCGCCCCTCTCGTCCCCATCATCCGCGACAACCTCAACCTCACCAAGTCCGACATCGGCAACGCCGGCGTCACCTCCGTCTCCGGCAGCATCTTCTCCCGTCTCGTCATGGGCGCCGTCTGCGACCTCCTCGGCCCCCGCTACGGCTGCGCCTTCCTCATCATGCTCTCCGCCCCCACCGTCTTCTGCATGTCCTTCGTCTCCTCCCCCGCCGGCTACATCGCCGTCCGCTTCATGATCGGCTTCTCCCTCGCCACCTTCGTCTCCTGCCAGTACTGGATGAGCACCATGTTCAACAGCCAGATCATCGGCCTCGTCAACGGCACCGCCGCCGGCTGGGGCAACATGGGCGGAGGCGCCACCCAGCTCCTCATGCCTCTCCTCTACGAGATCATCAAGAAGGTCGGGGCCACCCCCTTCACCGCCTGGAGGATCGCCTTCTTCATCCCCGGCTCCCTCCACGTCCTCATGGGCATTTTGGTCATGACTCTCGGCCAGGACTTGCCCGACGGAAATCTCAGCAGTCTCCAAAAGAAGGGCACCGTGGCCAAGGATAAATTTTCCAACGTGCTGTGGTACGCGGTCACCAACTACAGAACTTGGATCTTCGTTCTCCTGTATGGCTACTCAATGGGCGTCGAGCTCTCCACCGACAACGTCGTCGCCGAGTACTTCTACGACAGGTTCGACCTGAAGCTGCACACGGCGGGAATCATCGCAGCCACCTTCGGGATGGCGAATCTAGTGGCTCGCCCCTTCGGAGGGTACGCTTCCGACTTCGCAGCTCGATATTTCGGGATGAGGGGAAGGCTGTGGACCCTGTGGATCCTCCAAACGTGCGGTGGAGTGTTCTGCATGTGGCTTGGGAAGGCAACCCAGCTTCCAGTGGCCATAGTGGCCATGATCCTGTTCTCAATAGGAGCCCAGGCTGCGTGCGGAGCCACGTTTGGGATCATTCCCTTCATTTCCCGGCGCTCGTTGGGCATTATATCGGGCCTCACAGGCGCAGGAGGCAACTTCGGGTCTGGGTTGACTCAATTGGTGTTCTTCTCGACAACGAAATACTCCACAGACAAAGGGTTGTTCTTGATGGGCATTATGATCGTGTGCTGCACTCTTCCGGTCACATTGGTTCACTTCCCGCAGTGGGGGAGCATGTTCTTGCCGCCCACCAAACACGTGGAGAAATCCACAGAAGAATTCTATTATGGGTCTGAATGGACTGAGGACGAGAAGAAGAAGGGTCTGCACCAGCAGAGCATCAAGTTCGCTGAGAATAGCAGGTCCGAACGTGGCAGGAAAATTGCGTCAGCTCCAACCCCACCCAACGCCACTCCTTCCCATGTT

Protein sequence

GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV

Homology

[Close]

BLAST of MC03g0332 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 1.2e-249
Identity = 419/525 (79.81%), Postives = 472/525 (89.90%), Query Frame = 0


Query: 5   GSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMRTF 64
           G PGSSMHGVTGREQ++AFSV SP VP+D+SAKF+LPVD+EHKAKVFK+ S   PHMRTF
Sbjct: 6   GEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAPHMRTF 65

Query: 65  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCDLL 124
           HL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGAVCDLL
Sbjct: 66  HLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLL 125

Query: 125 GPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 184
           GPRYGCAFL+MLSAPTVF MSFV    GYI VRFMIGF LATFVSCQYWMSTMFN QIIG
Sbjct: 126 GPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIG 185

Query: 185 LVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVMTL 244
           LVNGTAAGWGNMGGG TQLLMP++YEII+++G+T FTAWR+AFF+PG +H++MGILV+TL
Sbjct: 186 LVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTL 245

Query: 245 GQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVAEY 304
           GQDLPDGN S+L+KKG V KDKFS VLWYA+TNYRTW+FVLLYGYSMGVEL+TDNV+AEY
Sbjct: 246 GQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEY 305

Query: 305 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVF 364
           F+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD AAR FGMRGRLWTLWI+QT GG F
Sbjct: 306 FFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFF 365

Query: 365 CMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 424
           C+WLG+AT LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNFGSGL
Sbjct: 366 CLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGL 425

Query: 425 TQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 484
           TQLVFFST+ +ST++GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTEE+YY 
Sbjct: 426 TQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEEYYYM 485

Query: 485 SEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 530
            EWTE EK+KG+H+ S+KFA NSRSERGR++ASAP+PP   P HV
Sbjct: 486 KEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

[Close]

BLAST of MC03g0332 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 2.4e-247
Identity = 417/529 (78.83%), Postives = 470/529 (88.85%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+  G PGSSMHGVTGREQ+FAFSV SPIV TD +AKF LPVD+EHKA VFK+FS A PH
Sbjct: 2   GDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFL+MLSAPTVF MSFVS  AG+I VRFMIGF LATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGG TQLLMP++YEII++ G+T FTAWRIAFF+PG LH++MGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+ LGQDLPDGN ++L+K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASDFAA+YFGMRGRLWTLWI+QT 
Sbjct: 302 IAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTA 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GG+FC+WLG+A  L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 362 GGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQL+FFST+ ++T++GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE 
Sbjct: 422 GSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEA 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 530
            YYGSEW E EK+K +HQ S++FAEN++SE GR++ SA TPP  TP++V
Sbjct: 482 HYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

[Close]

BLAST of MC03g0332 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 3.6e-235
Identity = 401/529 (75.80%), Postives = 454/529 (85.82%), Query Frame = 0


Query: 5   GSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMRTF 64
           G+PGSS+HGVTGRE  FAFS          ++KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 65  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCDLL 124
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK+DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 125 GPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 184
           GPRYGCAFLIML+APTVFCMS + S AGYIAVRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 185 LVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVMTL 244
           LVNG AAGWGNMGGGATQL+MPL+Y++I+K GATPFTAWR+A+F+PG+LHV+MG+LV+TL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 245 GQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVAEY 304
           GQDLPDGNL SLQKKG V +D FS VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNV+AEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 305 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVF 364
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD  ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 365 CMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 424
           C+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 425 TQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 484
           TQL+FF++++YST  GL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 485 SEWTEDEKKKGLHQQSIKFAENSRSERGRK-----IASAPTPPNATPSH 529
           SEW+E EK KGLH  S+KFAENSRSERGR+      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

[Close]

BLAST of MC03g0332 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 3.6e-235
Identity = 401/529 (75.80%), Postives = 454/529 (85.82%), Query Frame = 0


Query: 5   GSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMRTF 64
           G+PGSS+HGVTGRE  FAFS          ++KF LPVDSEHKAK  ++ S ANPHMRTF
Sbjct: 7   GAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFANPHMRTF 66

Query: 65  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCDLL 124
           HLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK+DIGNAGV SVSGSIFSRL MGA+CD+L
Sbjct: 67  HLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMGAICDML 126

Query: 125 GPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 184
           GPRYGCAFLIML+APTVFCMS + S AGYIAVRF+IGFSLATFVSCQYWMSTMFNS+IIG
Sbjct: 127 GPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMFNSKIIG 186

Query: 185 LVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVMTL 244
           LVNG AAGWGNMGGGATQL+MPL+Y++I+K GATPFTAWR+A+F+PG+LHV+MG+LV+TL
Sbjct: 187 LVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMGVLVLTL 246

Query: 245 GQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVAEY 304
           GQDLPDGNL SLQKKG V +D FS VLWYAVTNYRTWIFVLLYGYSMGVEL+TDNV+AEY
Sbjct: 247 GQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTDNVIAEY 306

Query: 305 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVF 364
           FYDRFDL L  AGIIAA+FGMAN+VARP GG  SD  ARYFGMR RLW +WILQT GG F
Sbjct: 307 FYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQTAGGAF 366

Query: 365 CMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 424
           C+ LG+A+ LP ++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGGNFG+GL
Sbjct: 367 CLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGGNFGAGL 426

Query: 425 TQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 484
           TQL+FF++++YST  GL  MGIMI+ CTLPV LVHFPQWGSMFLPP    E   EE YYG
Sbjct: 427 TQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE---EEHYYG 486

Query: 485 SEWTEDEKKKGLHQQSIKFAENSRSERGRK-----IASAPTPPNATPSH 529
           SEW+E EK KGLH  S+KFAENSRSERGR+      A+A TPPN +P H
Sbjct: 487 SEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

[Close]

BLAST of MC03g0332 vs. ExPASy Swiss-Prot
Match: Q9FJH7 (High affinity nitrate transporter 2.3 OS=Arabidopsis thaliana OX=3702 GN=NRT2.3 PE=1 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.3e-228
Identity = 388/533 (72.80%), Postives = 452/533 (84.80%), Query Frame = 0


Query: 2   NVEGSPGSSMHGVTGREQTFAFSV---ASPIVPTDTSAKFALPVDSEHKAKVFKIFSLAN 61
           N EGS G+S+HGVT REQ F+FSV   +  +   D +AKFALPVDSEH+AKVF   S A 
Sbjct: 7   NEEGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAK 66

Query: 62  PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMG 121
           PHMR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK+DIGNAGV SVSG+IFSRL MG
Sbjct: 67  PHMRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMG 126

Query: 122 AVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMF 181
           AVCDLLG RYG AF +ML+APTVF MSFV  P+GY+ VRFMIGF LATFVSCQYW S MF
Sbjct: 127 AVCDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMF 186

Query: 182 NSQIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMG 241
           N +IIGLVNG A GWG+MGGG TQLLMP+++ +IK  GATPF AWRIAFF+PG L V+MG
Sbjct: 187 NGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMG 246

Query: 242 ILVMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTD 301
           ILV++LGQDLPDGNLS+LQK G V+KDKFS V W+AV NYRTWI  +LYG SMG+EL+ +
Sbjct: 247 ILVLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTIN 306

Query: 302 NVVAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQ 361
           NV++ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASD AAR FGMRGRLWTLWI Q
Sbjct: 307 NVISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQ 366

Query: 362 TCGGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 421
           T G +FC+WLG+A+ LP+AI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGG
Sbjct: 367 TVGALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGG 426

Query: 422 NFGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKST 481
           NFGSGLTQL+FFS+ ++ST +GL LMG+M V CTLPV  +HFPQWGSMFL P+   E+S 
Sbjct: 427 NFGSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQ 486

Query: 482 EEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASA--PTPPNATPSHV 530
           EE+YYGSEWTE+EK++GLH+ SIKFAENSRSERGRK+A A  PTP N TPSHV
Sbjct: 487 EEYYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

[Close]

BLAST of MC03g0332 vs. NCBI nr
Match: XP_022152832.1 (high affinity nitrate transporter 2.4-like [Momordica charantia])

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH
Sbjct: 6   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 65

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 66  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 125

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 126 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 185

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL
Sbjct: 186 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 245

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 246 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 305

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC
Sbjct: 306 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 365

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 366 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 425

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 426 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 485

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV
Sbjct: 486 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

[Close]

BLAST of MC03g0332 vs. NCBI nr
Match: XP_031736393.1 (high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP53714.1 high affinity nitrate transporter 2.1 [Cucumis sativus] >KAE8652752.1 hypothetical protein Csa_014056 [Cucumis sativus])

HSP 1 Score: 1014 bits (2621), Expect = 0.0
Identity = 502/529 (94.90%), Postives = 519/529 (98.11%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVF+I+SLANPH
Sbjct: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLYE+I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKAT+LP+AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TP+HV
Sbjct: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of MC03g0332 vs. NCBI nr
Match: AGO64298.1 (high-affinity nitrate transporter NRT2.2 [Cucumis sativus])

HSP 1 Score: 1013 bits (2619), Expect = 0.0
Identity = 501/529 (94.71%), Postives = 518/529 (97.92%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVF+I+SLANPH
Sbjct: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS  AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLYE+I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKAT+LP+AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TP+HV
Sbjct: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of MC03g0332 vs. NCBI nr
Match: NP_001274401.1 (high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-affinity nitrate transporter [Cucumis sativus])

HSP 1 Score: 1012 bits (2616), Expect = 0.0
Identity = 502/529 (94.90%), Postives = 518/529 (97.92%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVF+I+SLANPH
Sbjct: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLYE+I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKAT+LP+AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPS V
Sbjct: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

[Close]

BLAST of MC03g0332 vs. NCBI nr
Match: XP_023520099.1 (high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo] >XP_023520141.1 high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1010 bits (2612), Expect = 0.0
Identity = 500/529 (94.52%), Postives = 514/529 (97.16%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVFK+FS ANPH
Sbjct: 3   GGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPH 62

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 63  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 122

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS  AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 123 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 182

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLY+IIK+ GATPFTAWRIAFFIPG LHV+MGIL
Sbjct: 183 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGIL 242

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNLSSLQKKG VAKDKFS VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 243 VLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNV 302

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT 
Sbjct: 303 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 362

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKA +LP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 363 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 422

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKY+TDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 423 GSGLTQLVFFSTTKYTTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 482

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYG+EWTEDEKKKGLHQQSIKFAENSRSERGR++ SAPTPPN TPSHV
Sbjct: 483 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 531

[Close]

BLAST of MC03g0332 vs. ExPASy TrEMBL
Match: A0A6J1DHB7 (high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC111020453 PE=4 SV=1)

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH
Sbjct: 6   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 65

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 66  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 125

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 126 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 185

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL
Sbjct: 186 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 245

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 246 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 305

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC
Sbjct: 306 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 365

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 366 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 425

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 426 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 485

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV
Sbjct: 486 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 534

[Close]

BLAST of MC03g0332 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 1014 bits (2621), Expect = 0.0
Identity = 502/529 (94.90%), Postives = 519/529 (98.11%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVF+I+SLANPH
Sbjct: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLYE+I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKAT+LP+AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TP+HV
Sbjct: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of MC03g0332 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 1013 bits (2619), Expect = 0.0
Identity = 501/529 (94.71%), Postives = 518/529 (97.92%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVF+I+SLANPH
Sbjct: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS  AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLYE+I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKAT+LP+AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TP+HV
Sbjct: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

[Close]

BLAST of MC03g0332 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 1012 bits (2616), Expect = 0.0
Identity = 502/529 (94.90%), Postives = 518/529 (97.92%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVF+I+SLANPH
Sbjct: 2   GDVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS+ AGYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGGATQL+MPLLYE+I++ GATPFTAWRIAFFIPG LHVLMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+TLGQDLPDGNL+SLQKKG VAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQT 
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTF 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GGVFCMWLGKAT+LP+AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQLVFFSTTKYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGR++ASAPTPPN TPS V
Sbjct: 482 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

[Close]

BLAST of MC03g0332 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 1009 bits (2610), Expect = 0.0
Identity = 500/527 (94.88%), Postives = 513/527 (97.34%), Query Frame = 0


Query: 3   VEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMR 62
           VEGSPGSSMHGVTGREQTFAFSVASPIVPTDT+AKFALPVDSEHKAKVFK+FS ANPHMR
Sbjct: 4   VEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPHMR 63

Query: 63  TFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCD 122
           TFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTK DIGNAGVTSVSGSIFSRLVMGAVCD
Sbjct: 64  TFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCD 123

Query: 123 LLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQI 182
           LLGPRYGCAFLIMLSAPTVFCMSFVS  AGYIAVRFMIGFSLATFVSCQYWMSTMFNSQI
Sbjct: 124 LLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQI 183

Query: 183 IGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVM 242
           IGLVNGTAAGWGNMGGGATQL+MPLLY+IIK+ GATPFTAWRIAFFIPG LHV+MGILV+
Sbjct: 184 IGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGILVL 243

Query: 243 TLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVA 302
           TLGQDLPDGNLSSLQKKG VAKDKFS VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV+A
Sbjct: 244 TLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNVIA 303

Query: 303 EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGG 362
           EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQT GG
Sbjct: 304 EYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTFGG 363

Query: 363 VFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS 422
           VFCMWLGKA +LP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS
Sbjct: 364 VFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGS 423

Query: 423 GLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY 482
           GLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY
Sbjct: 424 GLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFY 483

Query: 483 YGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 529
           YG+EWTEDEKKKGLHQQSIKFAENSRSERGR++ SAPTPPN TPSHV
Sbjct: 484 YGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

[Close]

BLAST of MC03g0332 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 863.6 bits (2230), Expect = 8.2e-251
Identity = 419/525 (79.81%), Postives = 472/525 (89.90%), Query Frame = 0


Query: 5   GSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMRTF 64
           G PGSSMHGVTGREQ++AFSV SP VP+D+SAKF+LPVD+EHKAKVFK+ S   PHMRTF
Sbjct: 6   GEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAPHMRTF 65

Query: 65  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCDLL 124
           HL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGAVCDLL
Sbjct: 66  HLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLL 125

Query: 125 GPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 184
           GPRYGCAFL+MLSAPTVF MSFV    GYI VRFMIGF LATFVSCQYWMSTMFN QIIG
Sbjct: 126 GPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIG 185

Query: 185 LVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVMTL 244
           LVNGTAAGWGNMGGG TQLLMP++YEII+++G+T FTAWR+AFF+PG +H++MGILV+TL
Sbjct: 186 LVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTL 245

Query: 245 GQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVAEY 304
           GQDLPDGN S+L+KKG V KDKFS VLWYA+TNYRTW+FVLLYGYSMGVEL+TDNV+AEY
Sbjct: 246 GQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEY 305

Query: 305 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVF 364
           F+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD AAR FGMRGRLWTLWI+QT GG F
Sbjct: 306 FFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFF 365

Query: 365 CMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 424
           C+WLG+AT LP A+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNFGSGL
Sbjct: 366 CLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGL 425

Query: 425 TQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYG 484
           TQLVFFST+ +ST++GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTEE+YY 
Sbjct: 426 TQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEEYYYM 485

Query: 485 SEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 530
            EWTE EK+KG+H+ S+KFA NSRSERGR++ASAP+PP   P HV
Sbjct: 486 KEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

[Close]

BLAST of MC03g0332 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-248
Identity = 417/529 (78.83%), Postives = 470/529 (88.85%), Query Frame = 0


Query: 1   GNVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPH 60
           G+  G PGSSMHGVTGREQ+FAFSV SPIV TD +AKF LPVD+EHKA VFK+FS A PH
Sbjct: 2   GDSTGEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKPH 61

Query: 61  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAV 120
           MRTFHLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAV 121

Query: 121 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 180
           CDLLGPRYGCAFL+MLSAPTVF MSFVS  AG+I VRFMIGF LATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNS 181

Query: 181 QIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGIL 240
           QIIGLVNGTAAGWGNMGGG TQLLMP++YEII++ G+T FTAWRIAFF+PG LH++MGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGIL 241

Query: 241 VMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 300
           V+ LGQDLPDGN ++L+K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLNLGQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 301 VAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTC 360
           +AEYF+DRF LKLHTAG+IAA FGMAN  ARP GGYASDFAA+YFGMRGRLWTLWI+QT 
Sbjct: 302 IAEYFFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTA 361

Query: 361 GGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 420
           GG+FC+WLG+A  L  A+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNF
Sbjct: 362 GGLFCVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNF 421

Query: 421 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 480
           GSGLTQL+FFST+ ++T++GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE 
Sbjct: 422 GSGLTQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEA 481

Query: 481 FYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNATPSHV 530
            YYGSEW E EK+K +HQ S++FAEN++SE GR++ SA TPP  TP++V
Sbjct: 482 HYYGSEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

[Close]

BLAST of MC03g0332 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 792.3 bits (2045), Expect = 2.3e-229
Identity = 388/533 (72.80%), Postives = 452/533 (84.80%), Query Frame = 0


Query: 2   NVEGSPGSSMHGVTGREQTFAFSV---ASPIVPTDTSAKFALPVDSEHKAKVFKIFSLAN 61
           N EGS G+S+HGVT REQ F+FSV   +  +   D +AKFALPVDSEH+AKVF   S A 
Sbjct: 7   NEEGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFAK 66

Query: 62  PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMG 121
           PHMR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK+DIGNAGV SVSG+IFSRL MG
Sbjct: 67  PHMRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAMG 126

Query: 122 AVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMF 181
           AVCDLLG RYG AF +ML+APTVF MSFV  P+GY+ VRFMIGF LATFVSCQYW S MF
Sbjct: 127 AVCDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVMF 186

Query: 182 NSQIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMG 241
           N +IIGLVNG A GWG+MGGG TQLLMP+++ +IK  GATPF AWRIAFF+PG L V+MG
Sbjct: 187 NGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVMG 246

Query: 242 ILVMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTD 301
           ILV++LGQDLPDGNLS+LQK G V+KDKFS V W+AV NYRTWI  +LYG SMG+EL+ +
Sbjct: 247 ILVLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTIN 306

Query: 302 NVVAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQ 361
           NV++ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASD AAR FGMRGRLWTLWI Q
Sbjct: 307 NVISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIFQ 366

Query: 362 TCGGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 421
           T G +FC+WLG+A+ LP+AI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAGG
Sbjct: 367 TVGALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAGG 426

Query: 422 NFGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKST 481
           NFGSGLTQL+FFS+ ++ST +GL LMG+M V CTLPV  +HFPQWGSMFL P+   E+S 
Sbjct: 427 NFGSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERSQ 486

Query: 482 EEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRKIASA--PTPPNATPSHV 530
           EE+YYGSEWTE+EK++GLH+ SIKFAENSRSERGRK+A A  PTP N TPSHV
Sbjct: 487 EEYYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

[Close]

BLAST of MC03g0332 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 783.1 bits (2021), Expect = 1.4e-226
Identity = 385/518 (74.32%), Postives = 448/518 (86.49%), Query Frame = 0


Query: 7   PGSSMHGVTGREQTFAFSVASPIVPTDTSAKFALPVDSEHKAKVFKIFSLANPHMRTFHL 66
           PGSSMHGVTGREQ++AFSV     PT+T  K+ LPVD+E KA VFK+FS A PHMRTFHL
Sbjct: 7   PGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKPHMRTFHL 66

Query: 67  SWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRLVMGAVCDLLGP 126
           SWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAVCDLLGP
Sbjct: 67  SWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAVCDLLGP 126

Query: 127 RYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLV 186
           RYGCAFL+MLSAPTVF MSFVS  AG+I VRFMIGF LATFVSCQYWMSTMFNSQIIGLV
Sbjct: 127 RYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIGLV 186

Query: 187 NGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHVLMGILVMTLGQ 246
           NGTAAGWGNMGGG TQLLMP++YEII++ G+T FTAWRIAFF+PG LH++MGILV+TLGQ
Sbjct: 187 NGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLTLGQ 246

Query: 247 DLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVVAEYFY 306
           DLP GN ++++K G VAKDKF  +LWYAVTNYRTWIFVLLYGYSMGVELSTDNV+AEYF+
Sbjct: 247 DLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFF 306

Query: 307 DRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTCGGVFCM 366
           DRF LKLHTAGIIAA FGMAN  ARP GG+ASD AA+ FGMRGRLWTLWI+QT GG+FC+
Sbjct: 307 DRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSGGLFCV 366

Query: 367 WLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQ 426
           WLG+A  L  A+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFGSGLTQ
Sbjct: 367 WLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQ 426

Query: 427 LVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEFYYGSE 486
           LVFFST++++T++GL  MG+MIV CTLPVTL+HFPQWGSMF PP+     +TE +Y G E
Sbjct: 427 LVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHYYVG-E 486

Query: 487 WTEDEKKKGLHQQSIKFAENSRSERGRKIASAPTPPNA 525
           ++++E++ G+H +S  FA+ +++E G  +       NA
Sbjct: 487 YSKEEQQIGMHLKSKLFADGAKTEGGSSVHKGNATNNA 522

[Close]

BLAST of MC03g0332 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 776.2 bits (2003), Expect = 1.7e-224
Identity = 381/536 (71.08%), Postives = 447/536 (83.40%), Query Frame = 0


Query: 2   NVEGSPGSSMHGVTGREQTFAFSV------ASPIVPTDTSAKFALPVDSEHKAKVFKIFS 61
           N +GS G+S+HGVT REQ F+FSV      +  +   D +AKFALPVDSEH+AKVFK  S
Sbjct: 7   NEDGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPLS 66

Query: 62  LANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKSDIGNAGVTSVSGSIFSRL 121
            A PHMR FHL WISFFTCF+STFAAAPLVP+IRDNL+LTK+DIGNAGV SVSG+IFSRL
Sbjct: 67  FAKPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSRL 126

Query: 122 VMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSPAGYIAVRFMIGFSLATFVSCQYWMS 181
            MGAVCDLLG RYG AF +ML+AP VF MSFV+    Y+AVRFMIGF LATFVSCQYW S
Sbjct: 127 AMGAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWTS 186

Query: 182 TMFNSQIIGLVNGTAAGWGNMGGGATQLLMPLLYEIIKKVGATPFTAWRIAFFIPGSLHV 241
            MF  +IIGLVNG A GWG+MGGG TQLLMP+++ +IK  GATPFTAWR AFFIPG L +
Sbjct: 187 VMFTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQI 246

Query: 242 LMGILVMTLGQDLPDGNLSSLQKKGTVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVEL 301
           +MGILV+TLGQDLPDGNLS+LQK G V+KDKFS V W+AV NYRTWI  +LYG+SMGVEL
Sbjct: 247 VMGILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVEL 306

Query: 302 STDNVVAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLW 361
           + +NV++ YFYDRF+L LHTAGIIAA+FGMAN  ARPFGGYASD AAR FGMRGRLW LW
Sbjct: 307 TINNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWILW 366

Query: 362 ILQTCGGVFCMWLGKATQLPVAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTG 421
           ILQT G +FC+WLG+A+ LP+AI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLTG
Sbjct: 367 ILQTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLTG 426

Query: 422 AGGNFGSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVE 481
           AGGNFGSG+TQL+FFS++++ST +GL LMG+M V C+LPV  +HFPQWGSMFL P++  E
Sbjct: 427 AGGNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDGE 486

Query: 482 KSTEEFYYGSEWTEDEKKKGLHQQSIKFAENSRSERGRK--IASAPTPPNATPSHV 530
           KS EE YYG+EWTE+EK  GLH+ SIKFAENSRSERGRK  +A  PTP   +P+HV
Sbjct: 487 KSKEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:

BLAST of MC03g0332 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJH8	1.2e-249	79.81	High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ...	[more]
O82811	2.4e-247	78.83	High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ...	[more]
P0DKG9	3.6e-235	75.80	High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P0DKH0	3.6e-235	75.80	High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9FJH7	3.3e-228	72.80	High affinity nitrate transporter 2.3 OS=Arabidopsis thaliana OX=3702 GN=NRT2.3 ...	[more]

BLAST of MC03g0332 vs. NCBI nr


Match Name	Identity	Description
XP_022152832.1	100.00	high affinity nitrate transporter 2.4-like [Momordica charantia]	[more]
XP_031736393.1	94.90	high affinity nitrate transporter 2.4-like isoform X1 [Cucumis sativus] >AZP5371...	[more]
AGO64298.1	94.71	high-affinity nitrate transporter NRT2.2 [Cucumis sativus]	[more]
NP_001274401.1	94.90	high affinity nitrate transporter 2.4-like [Cucumis sativus] >AAS93686.3 high-af...	[more]
XP_023520099.1	94.52	high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo] >XP_0235...	[more]

BLAST of MC03g0332 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1DHB7	100.00	high affinity nitrate transporter 2.4-like OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A3Q9CZ42	94.90	High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ...	[more]
S4VM42	94.71	High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE...	[more]
Q6PRP8	94.90	High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3	[more]
A0A6J1HIH1	94.88	high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of MC03g0332 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60770.1	8.2e-251	79.81	nitrate transporter 2.4	[more]
AT1G08090.1	1.7e-248	78.83	nitrate transporter 2:1	[more]
AT5G60780.1	2.3e-229	72.80	nitrate transporter 2.3	[more]
AT1G08100.1	1.4e-226	74.32	nitrate transporter 2.2	[more]
AT3G45060.1	1.7e-224	71.08	high affinity nitrate transporter 2.6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 68..424 e-value: 3.6E-21 score: 75.5
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 57..252 e-value: 4.9E-22 score: 80.3
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 263..465 e-value: 2.6E-27 score: 97.6
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 58..459
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 491..512
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 491..529
None	No IPR available	PANTHER	PTHR23515:SF16	HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKE	coord: 2..526
IPR044772	Nitrate transporter	PANTHER	PTHR23515	HIGH-AFFINITY NITRATE TRANSPORTER 2.3	coord: 2..526
IPR044772	Nitrate transporter	CDD	cd17341	MFS_NRT2_like	coord: 64..457 e-value: 5.72733E-137 score: 399.704

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0332.1	MC03g0332.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071249	cellular response to nitrate
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0031204	posttranslational protein targeting to membrane, translocation
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009705	plant-type vacuole membrane
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0071261	Ssh1 translocon complex
molecular_function	GO:0015112	nitrate transmembrane transporter activity
molecular_function	GO:0008320	protein transmembrane transporter activity
molecular_function	GO:0022857	transmembrane transporter activity