MC03g0325 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0325
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	MC03: 9581501 .. 9583243 (+)
RNA-Seq Expression	MC03g0325
Synteny	MC03g0325

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACGACGGAGCCGCCGAAGCATGGATTTTTATCAGACAAGTTGCGCGGGGACGGTGGTTCTCGGTGTTCGCGTCCTTCATGATCATGATCGGCGCCGGCTCAACTTACATGTTCGGGACTTACTCCAAAGCCATAAAGACCGAATTCAATTACAGCCAAACCGAAGTTAACACATTGGGATTCGCCAAGGACCTCGGCTCGAACGTCGGAGTTTTCGCCGGTCTTCTCGCCGAGGTGGCTCCGCTGTGGGTCCTCTTCATCGTCGGCTCAGCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAAGCCCGAATTCTGGCACATGTTCATCTACATTTGCTTGGCTACCAACTCACAGAATTTCGCCAACACCGCGGTTTTGGTCACCAGCGTCAGAAACTTCCCCGACCGAAGAGGGATCATTCTGGGGCTTCTCAAGGGCTTCGTTGGCATCGGAGGAGCCATCTTGACCCAGTTGTATTTAGCCGTGTACGGCCATGAAGACCCATCGAATCTTATTCTCCTTCTTTCGTGGTTCCCCTCATTCATATCTCTTTTGCTTTTGCTCGCAATTCGACCAATCAAAATCCGCAAACATCCCGATGAGCTAAAAGTGCTTTACCACTTGCTTTACGTCTCCATACTTCTGGCTTTGTTTATCTTGTTCTTGACCTTAATGCAAAAACAAGTGGTTTTCTCTCACACTGGATATGCGAGTGGCGCTTTCGCCATTTTGGCCTTGTTGGCTCTCCCCCTTTTGATCACTGTTAGAGAAGAGCTCATGCTCTACAAACTCAACAAACAAAACAATGACCCATCCGTCATCGTTTCCATCGTTGGCGAGCAGAAGCTCCCGATGGTAACCGTGCCCCAGAACACGCCGCCGACGTCCACGCTGCAAGAAATTACCGAAATCTCGCCGTCCTGCCTCGAAAACATATGCAACAAGCCTGAAAGAGGGGAGGATTTCACCATCTTGCAAGCCATCTTCAGCGTAGACATGGCTCTTGTCTATCTCGCCACCTTTACTGGCTGTGGCTCCTCCCTTGCTTCCATTGACAACATAGGCCAAATTGGTGAGTCTCTTCACTACCCAACTCAATCCATAGCCATTTTCGTCTCTTGGGTTTCCGTTTTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTCATGACCAAATACAAATTACCTCGCCCTCTCATGTTTGCTTTTTCCTTTCTCCTCACTTGCATCGGCCAGCTCTTCATCGCCTTCCCATCTCCTGGCTCGGTCTACGTCGCTTCCTTGATCATTGGGTTCGGGTTCGGAGCCCAAGTTCCCCTGTTGTTCGCCATAATCTCCGAGCTCTTTGGTCTCAAACGCTACTCAACGCTCTTTAACTGTGGACAACTGGTGGTCCCGTTTGGATCCTATATACTAAACGTGGATATAGTGGGGAAGTTGTACGATATTGAAGCCATAAAAGAAGGTAGTAAGAAGACTGGCAAGGGGCTAACTTGCACCGGGGCTCATTGCTTCGGCTGGTCTTTCACAATTTTGGCATCAGTGACATTGTTTGGAGCTCTAGTTGCGCTTGTGCTGGCTTATAGGACTCGAGAGTTTTACAAAGGCGACGTATATAAGAAATACAGAGAGGACATGTGGATTCCACAATCGGAAATGGAATTCTATAGGTTAGATAACAAGAAGAACATTGATGAT

mRNA sequence

ATGGCCAACGACGGAGCCGCCGAAGCATGGATTTTTATCAGACAAGTTGCGCGGGGACGGTGGTTCTCGGTGTTCGCGTCCTTCATGATCATGATCGGCGCCGGCTCAACTTACATGTTCGGGACTTACTCCAAAGCCATAAAGACCGAATTCAATTACAGCCAAACCGAAGTTAACACATTGGGATTCGCCAAGGACCTCGGCTCGAACGTCGGAGTTTTCGCCGGTCTTCTCGCCGAGGTGGCTCCGCTGTGGGTCCTCTTCATCGTCGGCTCAGCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAAGCCCGAATTCTGGCACATGTTCATCTACATTTGCTTGGCTACCAACTCACAGAATTTCGCCAACACCGCGGTTTTGGTCACCAGCGTCAGAAACTTCCCCGACCGAAGAGGGATCATTCTGGGGCTTCTCAAGGGCTTCGTTGGCATCGGAGGAGCCATCTTGACCCAGTTGTATTTAGCCGTGTACGGCCATGAAGACCCATCGAATCTTATTCTCCTTCTTTCGTGGTTCCCCTCATTCATATCTCTTTTGCTTTTGCTCGCAATTCGACCAATCAAAATCCGCAAACATCCCGATGAGCTAAAAGTGCTTTACCACTTGCTTTACGTCTCCATACTTCTGGCTTTGTTTATCTTGTTCTTGACCTTAATGCAAAAACAAGTGGTTTTCTCTCACACTGGATATGCGAGTGGCGCTTTCGCCATTTTGGCCTTGTTGGCTCTCCCCCTTTTGATCACTGTTAGAGAAGAGCTCATGCTCTACAAACTCAACAAACAAAACAATGACCCATCCGTCATCGTTTCCATCGTTGGCGAGCAGAAGCTCCCGATGGTAACCGTGCCCCAGAACACGCCGCCGACGTCCACGCTGCAAGAAATTACCGAAATCTCGCCGTCCTGCCTCGAAAACATATGCAACAAGCCTGAAAGAGGGGAGGATTTCACCATCTTGCAAGCCATCTTCAGCGTAGACATGGCTCTTGTCTATCTCGCCACCTTTACTGGCTGTGGCTCCTCCCTTGCTTCCATTGACAACATAGGCCAAATTGGTGAGTCTCTTCACTACCCAACTCAATCCATAGCCATTTTCGTCTCTTGGGTTTCCGTTTTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTCATGACCAAATACAAATTACCTCGCCCTCTCATGTTTGCTTTTTCCTTTCTCCTCACTTGCATCGGCCAGCTCTTCATCGCCTTCCCATCTCCTGGCTCGGTCTACGTCGCTTCCTTGATCATTGGGTTCGGGTTCGGAGCCCAAGTTCCCCTGTTGTTCGCCATAATCTCCGAGCTCTTTGGTCTCAAACGCTACTCAACGCTCTTTAACTGTGGACAACTGGTGGTCCCGTTTGGATCCTATATACTAAACGTGGATATAGTGGGGAAGTTGTACGATATTGAAGCCATAAAAGAAGGTAGTAAGAAGACTGGCAAGGGGCTAACTTGCACCGGGGCTCATTGCTTCGGCTGGTCTTTCACAATTTTGGCATCAGTGACATTGTTTGGAGCTCTAGTTGCGCTTGTGCTGGCTTATAGGACTCGAGAGTTTTACAAAGGCGACGTATATAAGAAATACAGAGAGGACATGTGGATTCCACAATCGGAAATGGAATTCTATAGGTTAGATAACAAGAAGAACATTGATGAT

Coding sequence (CDS)

ATGGCCAACGACGGAGCCGCCGAAGCATGGATTTTTATCAGACAAGTTGCGCGGGGACGGTGGTTCTCGGTGTTCGCGTCCTTCATGATCATGATCGGCGCCGGCTCAACTTACATGTTCGGGACTTACTCCAAAGCCATAAAGACCGAATTCAATTACAGCCAAACCGAAGTTAACACATTGGGATTCGCCAAGGACCTCGGCTCGAACGTCGGAGTTTTCGCCGGTCTTCTCGCCGAGGTGGCTCCGCTGTGGGTCCTCTTCATCGTCGGCTCAGCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAAGCCCGAATTCTGGCACATGTTCATCTACATTTGCTTGGCTACCAACTCACAGAATTTCGCCAACACCGCGGTTTTGGTCACCAGCGTCAGAAACTTCCCCGACCGAAGAGGGATCATTCTGGGGCTTCTCAAGGGCTTCGTTGGCATCGGAGGAGCCATCTTGACCCAGTTGTATTTAGCCGTGTACGGCCATGAAGACCCATCGAATCTTATTCTCCTTCTTTCGTGGTTCCCCTCATTCATATCTCTTTTGCTTTTGCTCGCAATTCGACCAATCAAAATCCGCAAACATCCCGATGAGCTAAAAGTGCTTTACCACTTGCTTTACGTCTCCATACTTCTGGCTTTGTTTATCTTGTTCTTGACCTTAATGCAAAAACAAGTGGTTTTCTCTCACACTGGATATGCGAGTGGCGCTTTCGCCATTTTGGCCTTGTTGGCTCTCCCCCTTTTGATCACTGTTAGAGAAGAGCTCATGCTCTACAAACTCAACAAACAAAACAATGACCCATCCGTCATCGTTTCCATCGTTGGCGAGCAGAAGCTCCCGATGGTAACCGTGCCCCAGAACACGCCGCCGACGTCCACGCTGCAAGAAATTACCGAAATCTCGCCGTCCTGCCTCGAAAACATATGCAACAAGCCTGAAAGAGGGGAGGATTTCACCATCTTGCAAGCCATCTTCAGCGTAGACATGGCTCTTGTCTATCTCGCCACCTTTACTGGCTGTGGCTCCTCCCTTGCTTCCATTGACAACATAGGCCAAATTGGTGAGTCTCTTCACTACCCAACTCAATCCATAGCCATTTTCGTCTCTTGGGTTTCCGTTTTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTCATGACCAAATACAAATTACCTCGCCCTCTCATGTTTGCTTTTTCCTTTCTCCTCACTTGCATCGGCCAGCTCTTCATCGCCTTCCCATCTCCTGGCTCGGTCTACGTCGCTTCCTTGATCATTGGGTTCGGGTTCGGAGCCCAAGTTCCCCTGTTGTTCGCCATAATCTCCGAGCTCTTTGGTCTCAAACGCTACTCAACGCTCTTTAACTGTGGACAACTGGTGGTCCCGTTTGGATCCTATATACTAAACGTGGATATAGTGGGGAAGTTGTACGATATTGAAGCCATAAAAGAAGGTAGTAAGAAGACTGGCAAGGGGCTAACTTGCACCGGGGCTCATTGCTTCGGCTGGTCTTTCACAATTTTGGCATCAGTGACATTGTTTGGAGCTCTAGTTGCGCTTGTGCTGGCTTATAGGACTCGAGAGTTTTACAAAGGCGACGTATATAAGAAATACAGAGAGGACATGTGGATTCCACAATCGGAAATGGAATTCTATAGGTTAGATAACAAGAAGAACATTGATGAT

Protein sequence

MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD

Homology

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BLAST of MC03g0325 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.4e-27
Identity = 138/539 (25.60%), Postives = 231/539 (42.86%), Query Frame = 0


Query: 20  RWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVFAGLLA 79
           +W  + A+  I    G+ + F  YS  +K+    SQ  +N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 80  EVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYIC--LATNSQNFANTAVL 139
              PL V+    +A+ F  Y + WL +T  I  P   +  +++C  LA  S  + NTA  
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICWFNTACF 162

Query: 140 VTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLL-SWFP---SFI 199
           +  +R+FP+ R + L L   F GI  A+ +  + A+  +   SNL LLL S  P   SF 
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLNSLVPLVVSFA 222

Query: 200 SLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFA 259
           +L  +L    +      D  +   H+  +  +LA+   F  L+      S      GA  
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVV 282

Query: 260 ILAL-LALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQNTPPTSTLQEI 319
           +L   L  PLL+  R+  +     + N++ S  V         M+ + +     +++   
Sbjct: 283 LLVFPLCAPLLVYARDYFLPVINARLNHESSGYV---------MLNIDELKNQKTSVSSK 342

Query: 320 TEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASIDNIGQIGESL 379
           T           N    G++ +    I  ++  L Y+A F G    L   +N+GQI +SL
Sbjct: 343 TGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL 402

Query: 380 HYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAF 439
               Q+    V+  S F+FFGR+ S    + +  +++L R   FA + L T I    +A 
Sbjct: 403 ---GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAV 462

Query: 440 PS--PGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPFGSYILNVD 499
            S    ++  A+ +IG   G       +I S+LFG        N     +P GS +    
Sbjct: 463 SSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY- 522

Query: 500 IVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLAYRTREFY 550
           I   +Y+  A  + +      + C G  C+  +F     +++ G + +L L  RT+  Y
Sbjct: 523 IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

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BLAST of MC03g0325 vs. NCBI nr
Match: XP_022152846.1 (uncharacterized protein LOC111020470 [Momordica charantia])

HSP 1 Score: 903 bits (2333), Expect = 0.0
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0


Query: 118 MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 177
           MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE
Sbjct: 1   MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 60

Query: 178 DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 237
           DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ
Sbjct: 61  DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 120

Query: 238 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 297
           KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV
Sbjct: 121 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 180

Query: 298 TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 357
           TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS
Sbjct: 181 TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 240

Query: 358 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 417
           SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA
Sbjct: 241 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 300

Query: 418 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 477
           FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ
Sbjct: 301 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 360

Query: 478 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 537
           LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV
Sbjct: 361 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 420

Query: 538 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
           ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD
Sbjct: 421 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 464

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BLAST of MC03g0325 vs. NCBI nr
Match: XP_022152849.1 (uncharacterized protein LOC111020473 [Momordica charantia])

HSP 1 Score: 855 bits (2208), Expect = 8.59e-308
Identity = 430/577 (74.52%), Postives = 495/577 (85.79%), Query Frame = 0


Query: 3   NDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLG 62
           N+ AA +W F++QVA GRWFSVFASF+IMIGAGSTY+FGTYSK +KT+F+Y QT+VNTLG
Sbjct: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63

Query: 63  FAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYI 122
           FAKDLGSN GVFAGLLAEVAP WVLF+VGS+LNFFSYFMIWLSVTRRIA P+FWHM +YI
Sbjct: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123

Query: 123 CLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNL 182
           CLA NSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVGIGGAILTQ+YLA+YGHE+ +++
Sbjct: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASV 183

Query: 183 ILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVF 242
           ILLL+WFPS IS + +L+IR IK+RKHP+ELKV YHLLYVSI+LAL+ILFLTL QKQ VF
Sbjct: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243

Query: 243 SHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQN 302
           SH GY  GA  I+ LL LPLLI +REE ML+ LNKQN D  V V ++ +QK         
Sbjct: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAV-LIHDQK--------- 303

Query: 303 TPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASI 362
            PP   L+E        ++++  KPERG+DFTI QA+ S DMAL+++AT +GCGSS+A+I
Sbjct: 304 PPPKRALEE------DAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAI 363

Query: 363 DNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLL 422
           DNIGQI ESL YP  SIAIFVSWVS+FNFFGRVFSGFISETLM KYKLPRPLMFA SFLL
Sbjct: 364 DNIGQIAESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLL 423

Query: 423 TCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPF 482
           TCIGQL IAFP  GSVY ASLIIGFGFGAQVPLLFAIISELFGLK YSTLFNCGQL VPF
Sbjct: 424 TCIGQLLIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPF 483

Query: 483 GSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLA 542
           GSYILNVD++GKLYD+EAI++G KKTGKGLTCTGA+CF  SFTILA+VT FGAL +LVLA
Sbjct: 484 GSYILNVDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLA 543

Query: 543 YRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNI 579
           YRTR+FYKG+ YKKYREDMWIP+++MEFY LDN+K +
Sbjct: 544 YRTRDFYKGETYKKYREDMWIPKADMEFYCLDNRKAV 564

[Close]

BLAST of MC03g0325 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 848 bits (2190), Expect = 4.53e-305
Identity = 417/583 (71.53%), Postives = 494/583 (84.73%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C  TNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++Q YLA+YGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHP+ELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  +++LL +P+LI  REEL+LYKLNKQ   PSV VS+V           
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVH---------- 300

Query: 301 QNTP--PTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSS 360
           QN P  P S   EI EISPSC ENI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSS
Sbjct: 301 QNPPTLPASADSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSS 360

Query: 361 LASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAF 420
           LA+IDN+GQ+GESL YP ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL F+F
Sbjct: 361 LAAIDNLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSF 420

Query: 421 SFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQL 480
           +FL+TC+GQLFIA+P PGSVY+A++IIGFGFGAQ P+LFA+ISE+FGL+RYSTLFNCGQL
Sbjct: 421 AFLITCVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQL 480

Query: 481 VVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVA 540
            VP GSYILNVD+VGKLYD+EA++EG K  GKGLTCTGAHCFG SFTILA+ TLFGA+V 
Sbjct: 481 AVPLGSYILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVM 540

Query: 541 LVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
           LVLAYRTREFYK DVYK + E++WIPQ++MEFYRLD+KK+I+D
Sbjct: 541 LVLAYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of MC03g0325 vs. NCBI nr
Match: XP_022964774.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 847 bits (2188), Expect = 9.13e-305
Identity = 417/583 (71.53%), Postives = 492/583 (84.39%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C ATNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLA+YGH DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHPDELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  ++ LL +P+LI  REEL+LYKLNKQ   PSV +S++           
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLH---------- 300

Query: 301 QNTP--PTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSS 360
           QN P  PTS   EI EISPSC  NI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSS
Sbjct: 301 QNPPTLPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSS 360

Query: 361 LASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAF 420
           LA+IDN+GQ+GESL Y  ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL FAF
Sbjct: 361 LAAIDNLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAF 420

Query: 421 SFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQL 480
           +FL+TC+GQLFIA+P PGS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLKRYSTLFNCGQL
Sbjct: 421 AFLITCVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQL 480

Query: 481 VVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVA 540
             PFGSYILNVD+VGKLYD+EA++E  +  GKGLTCTGAHCFG SFT+LA+ TLFGA+V 
Sbjct: 481 AAPFGSYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVM 540

Query: 541 LVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
            VLAYRTREFYK DVYK + E++WIPQ+EMEFYRLDNKKNI+D
Sbjct: 541 FVLAYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of MC03g0325 vs. NCBI nr
Match: KAG6583526.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583533.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583538.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583544.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 847 bits (2188), Expect = 9.13e-305
Identity = 417/583 (71.53%), Postives = 492/583 (84.39%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +CL TNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLA+YGH DPS
Sbjct: 121 LVCLGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHPDELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  ++ LL +P+LI  REEL+LYKLNKQ   PSV +S++           
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLH---------- 300

Query: 301 QNTP--PTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSS 360
           QN P  PTS   EI EISPSC  NI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSS
Sbjct: 301 QNPPTLPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSS 360

Query: 361 LASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAF 420
           LA+IDN+GQ+GESL Y  ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL FAF
Sbjct: 361 LAAIDNLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAF 420

Query: 421 SFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQL 480
           +FL+TC+GQLFIA+P PGS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLKRYSTLFNCGQL
Sbjct: 421 AFLITCVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQL 480

Query: 481 VVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVA 540
             PFGSYILNVD+VGKLYD+EA++E  +  GKGLTCTGAHCFG SFT+LA+ TLFGA+V 
Sbjct: 481 AAPFGSYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVM 540

Query: 541 LVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
            VLAYRTREFYK DVYK + E++WIPQ+EMEFYRLDNKKNI+D
Sbjct: 541 FVLAYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of MC03g0325 vs. ExPASy TrEMBL
Match: A0A6J1DIZ3 (uncharacterized protein LOC111020470 OS=Momordica charantia OX=3673 GN=LOC111020470 PE=4 SV=1)

HSP 1 Score: 903 bits (2333), Expect = 0.0
Identity = 464/464 (100.00%), Postives = 464/464 (100.00%), Query Frame = 0


Query: 118 MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 177
           MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE
Sbjct: 1   MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 60

Query: 178 DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 237
           DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ
Sbjct: 61  DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 120

Query: 238 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 297
           KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV
Sbjct: 121 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 180

Query: 298 TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 357
           TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS
Sbjct: 181 TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 240

Query: 358 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 417
           SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA
Sbjct: 241 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 300

Query: 418 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 477
           FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ
Sbjct: 301 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 360

Query: 478 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 537
           LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV
Sbjct: 361 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 420

Query: 538 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
           ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD
Sbjct: 421 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 464

[Close]

BLAST of MC03g0325 vs. ExPASy TrEMBL
Match: A0A6J1DHB0 (uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020473 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 4.16e-308
Identity = 430/577 (74.52%), Postives = 495/577 (85.79%), Query Frame = 0


Query: 3   NDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLG 62
           N+ AA +W F++QVA GRWFSVFASF+IMIGAGSTY+FGTYSK +KT+F+Y QT+VNTLG
Sbjct: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63

Query: 63  FAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYI 122
           FAKDLGSN GVFAGLLAEVAP WVLF+VGS+LNFFSYFMIWLSVTRRIA P+FWHM +YI
Sbjct: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123

Query: 123 CLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNL 182
           CLA NSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVGIGGAILTQ+YLA+YGHE+ +++
Sbjct: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASV 183

Query: 183 ILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVF 242
           ILLL+WFPS IS + +L+IR IK+RKHP+ELKV YHLLYVSI+LAL+ILFLTL QKQ VF
Sbjct: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243

Query: 243 SHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQN 302
           SH GY  GA  I+ LL LPLLI +REE ML+ LNKQN D  V V ++ +QK         
Sbjct: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAV-LIHDQK--------- 303

Query: 303 TPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASI 362
            PP   L+E        ++++  KPERG+DFTI QA+ S DMAL+++AT +GCGSS+A+I
Sbjct: 304 PPPKRALEE------DAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAI 363

Query: 363 DNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLL 422
           DNIGQI ESL YP  SIAIFVSWVS+FNFFGRVFSGFISETLM KYKLPRPLMFA SFLL
Sbjct: 364 DNIGQIAESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLL 423

Query: 423 TCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPF 482
           TCIGQL IAFP  GSVY ASLIIGFGFGAQVPLLFAIISELFGLK YSTLFNCGQL VPF
Sbjct: 424 TCIGQLLIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPF 483

Query: 483 GSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLA 542
           GSYILNVD++GKLYD+EAI++G KKTGKGLTCTGA+CF  SFTILA+VT FGAL +LVLA
Sbjct: 484 GSYILNVDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLA 543

Query: 543 YRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNI 579
           YRTR+FYKG+ YKKYREDMWIP+++MEFY LDN+K +
Sbjct: 544 YRTRDFYKGETYKKYREDMWIPKADMEFYCLDNRKAV 564

[Close]

BLAST of MC03g0325 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 848 bits (2190), Expect = 2.19e-305
Identity = 417/583 (71.53%), Postives = 494/583 (84.73%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C  TNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++Q YLA+YGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHP+ELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  +++LL +P+LI  REEL+LYKLNKQ   PSV VS+V           
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVH---------- 300

Query: 301 QNTP--PTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSS 360
           QN P  P S   EI EISPSC ENI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSS
Sbjct: 301 QNPPTLPASADSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSS 360

Query: 361 LASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAF 420
           LA+IDN+GQ+GESL YP ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL F+F
Sbjct: 361 LAAIDNLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSF 420

Query: 421 SFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQL 480
           +FL+TC+GQLFIA+P PGSVY+A++IIGFGFGAQ P+LFA+ISE+FGL+RYSTLFNCGQL
Sbjct: 421 AFLITCVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQL 480

Query: 481 VVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVA 540
            VP GSYILNVD+VGKLYD+EA++EG K  GKGLTCTGAHCFG SFTILA+ TLFGA+V 
Sbjct: 481 AVPLGSYILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVM 540

Query: 541 LVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
           LVLAYRTREFYK DVYK + E++WIPQ++MEFYRLD+KK+I+D
Sbjct: 541 LVLAYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of MC03g0325 vs. ExPASy TrEMBL
Match: A0A6J1HP70 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464775 PE=4 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 4.42e-305
Identity = 417/583 (71.53%), Postives = 492/583 (84.39%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C ATNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLA+YGH DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHPDELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  ++ LL +P+LI  REEL+LYKLNKQ   PSV +S++           
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLH---------- 300

Query: 301 QNTP--PTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSS 360
           QN P  PTS   EI EISPSC  NI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSS
Sbjct: 301 QNPPTLPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSS 360

Query: 361 LASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAF 420
           LA+IDN+GQ+GESL Y  ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL FAF
Sbjct: 361 LAAIDNLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAF 420

Query: 421 SFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQL 480
           +FL+TC+GQLFIA+P PGS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLKRYSTLFNCGQL
Sbjct: 421 AFLITCVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQL 480

Query: 481 VVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVA 540
             PFGSYILNVD+VGKLYD+EA++E  +  GKGLTCTGAHCFG SFT+LA+ TLFGA+V 
Sbjct: 481 AAPFGSYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVM 540

Query: 541 LVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
            VLAYRTREFYK DVYK + E++WIPQ+EMEFYRLDNKKNI+D
Sbjct: 541 FVLAYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of MC03g0325 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 844 bits (2181), Expect = 5.13e-304
Identity = 415/583 (71.18%), Postives = 491/583 (84.22%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C  TNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLA+YGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHPDELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  ++ LL +P+LI  REEL+LYKLNK+   PSV +S++           
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLH---------- 300

Query: 301 QNTP--PTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSS 360
           QN P  PTS   EI EISPSC  NI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSS
Sbjct: 301 QNPPTLPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSS 360

Query: 361 LASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAF 420
           LA+IDN+GQ+GESL Y  ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL FAF
Sbjct: 361 LAAIDNLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAF 420

Query: 421 SFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQL 480
           +FL+TC+GQLFIA+P PGS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLKRYSTLFNCGQL
Sbjct: 421 AFLITCVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQL 480

Query: 481 VVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVA 540
             PFGSYILNVD+VGKLYD+EA++E  +  GKGLTCTGAHCFG SFT+LA+ TLFGA+V 
Sbjct: 481 AAPFGSYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVM 540

Query: 541 LVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 581
            VLAYRTREFYK DVYK + E++WIPQ+EMEFYRLDNKKNI+D
Sbjct: 541 FVLAYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of MC03g0325 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 589.3 bits (1518), Expect = 3.3e-168
Identity = 313/586 (53.41%), Postives = 414/586 (70.65%), Query Frame = 0


Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M ND   E   F+     GRWF VFASF+IM  AG+TY+FGTYSK IK+   Y QT +N 
Sbjct: 1   MGND---ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNL 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGF KDLG+NVGV +GL+AEV P W +  +GSA+NF  YFMIWL+VT ++AKP+ W M +
Sbjct: 61  LGFFKDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCL 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
           YIC+  NSQNFANT  LVT V+NFP+ RG++LGLLKG+VG+ GAI TQLY A+YGH D  
Sbjct: 121 YICIGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSK 180

Query: 181 NLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           +LILL++W P+ +SL+ +  IR  K+ +  +EL V Y  LY+SI LALF++ + + +KQV
Sbjct: 181 SLILLIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNK-QNNDPSVIVSIVGEQKLPMVTV 300
            FS   YA+ A    ALL +PL ++V++EL ++ + K    +PS +     + + P   +
Sbjct: 241 HFSKAAYAASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEV-----KVEKPKKEL 300

Query: 301 PQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSL 360
             +    + +    + + SC   + + P RGED+TILQA+ S DM ++++ATF G GSSL
Sbjct: 301 DLDQDKAAKVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSL 360

Query: 361 ASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFS 420
            ++DN+GQIGESL YP  +++ FVS VS++N+FGRVFSGF+SE L+ KYKLPRPLM    
Sbjct: 361 TAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLV 420

Query: 421 FLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLV 480
            LL+C G L IAFP PGSVY+AS+++GF FGAQ+PLLFAIISELFGLK YSTLFNCGQL 
Sbjct: 421 LLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLA 480

Query: 481 VPFGSYILNVDIVGKLYDIEAIKE-----GSKKTGKGLTCTGAHCFGWSFTILASVTLFG 540
            P GSYILNV + G LYD EA+K+      ++K  K LTC G+ C+   F ILA+VT FG
Sbjct: 481 SPLGSYILNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFG 540

Query: 541 ALVALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNID 581
           ALV+L LA RTREFYKGD+YKK+RE    P+SE E    D++K ++
Sbjct: 541 ALVSLGLAIRTREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

[Close]

BLAST of MC03g0325 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 546.2 bits (1406), Expect = 3.2e-155
Identity = 286/562 (50.89%), Postives = 385/562 (68.51%), Query Frame = 0


Query: 15  QVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVF 74
           Q+  GRWF  F S +IM  AG+TYMFG YS  IK    Y QT +N L F KDLG+NVGV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  AGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFANT 134
           AGLL EV P W + ++G+ LNFF YFMIWL+VT RI+KP+ WHM +YIC+  NSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 AVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSFIS 194
             LVT V+NFP+ RG++LG+LKG+VG+ GAI+TQLY A YG ED   LIL++ W P+ +S
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 195 LLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFAI 254
              L  IR +K+++  +ELKV Y+ LY+S+ LA F++ + ++ K   F+ + +   A  +
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 255 LALLALPLLITVREELMLYKLNK-QNNDPSVIVSIVGEQKLPMVTVPQNTPPTSTLQEIT 314
           + LL LP+++ + EE  L+K  +   NDP+ I  +  + KL       +    S  +E+ 
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEES--KEVV 315

Query: 315 E--ISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASIDNIGQIGES 374
           E   +PSC   + N PERG+D+TILQA+FSVDM +++LAT  G G +L +IDN+GQIG S
Sbjct: 316 EKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNS 375

Query: 375 LHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIA 434
           L YP +S++ FVS VS++N++GRV SG +SE  + KYK PRPLM     LL+C G L IA
Sbjct: 376 LGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIA 435

Query: 435 FPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPFGSYILNVDI 494
           F  PG +YVAS+IIGF FGAQ PLLFAIISE+FGLK YSTL+N G +  P GSY+LNV +
Sbjct: 436 FNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRV 495

Query: 495 VGKLYDIEAIKE-----GSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLAYRTR 554
            G LYD+EA K+      ++  G+ L C G  CF  SF I+A+VTLFG LV++VL  RT+
Sbjct: 496 AGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTK 555

Query: 555 EFYKGDVYKKYREDMWIPQSEM 569
           +FYK D+YKK+RE     + EM
Sbjct: 556 KFYKSDIYKKFREKALAAEMEM 574

[Close]

BLAST of MC03g0325 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 3.4e-80
Identity = 184/561 (32.80%), Postives = 299/561 (53.30%), Query Frame = 0


Query: 20  RWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVFAGLLA 79
           RW    A+  I   AG  Y+FG+ S  IK+  NY+Q E++ LG AKDLG +VG  AG L+
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 80  EVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFANTAVLVT 139
           E+ PLW   +VG+  N   Y  +WL VT R      W M + I +  N + + NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 140 SVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSFISLLLLL 199
            V+NFP  RG ++G+LKGF G+GGAI++Q+Y  ++   +P++LIL+++  P+ + + L+ 
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPASLILMVAVTPAVVVVCLMF 195

Query: 200 AIRPIKIRK--HPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFAILAL 259
            IRP+   K   P +      +  V +LLA +++ + L+Q  VV SH         +  +
Sbjct: 196 FIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVI 255

Query: 260 LALPLLITVR--------------EELMLYKLNKQN---NDPSVIVSIVGEQKLPMVTVP 319
           L +P+L+ +               EE ++ K   Q      P +I+S V ++K   V + 
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDL- 315

Query: 320 QNTPPTSTLQEITEISPSCLE---------NICNKPERGEDFTILQAIFSVDMALVYLAT 379
              P +   + I  +    ++         N    P RGEDFT+ QA+   D  L++ + 
Sbjct: 316 --LPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSL 375

Query: 380 FTGCGSSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLP 439
             G GS L  IDN+GQ+ +SL Y   +  + VS +S++NF GR+  G+ SE ++  Y  P
Sbjct: 376 LLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYP 435

Query: 440 RPLMFAFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYST 499
           RP+  A + L+  +G +F A+  PG++Y+ +L+IG G+GA   ++ A  SELFGLK++  
Sbjct: 436 RPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 495

Query: 500 LFNCGQLVVPFGSYILNVDIVGKLYDIEAIKEGSKKT---GKGLTCTGAHCFGWSFTILA 550
           L+N   L  P GS + +  I   +YD EA ++           L C G+ CF  +  I++
Sbjct: 496 LYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMS 555

[Close]

BLAST of MC03g0325 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 2.2e-79
Identity = 204/560 (36.43%), Postives = 307/560 (54.82%), Query Frame = 0


Query: 15  QVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVF 74
           ++ R +W ++ AS  I   +G++Y FG YS  +K+  +Y Q+ ++T+   KD+G+N GVF
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 75  AGLLAEVAPL----------------WVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHM 134
           +GLL   A                  WV+  VG+   F  YF+IW SVT  I KP    M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 135 FIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHED 194
            +++ LA  SQ F NTA +V++V NF D  G  +G++KGF+G+ GAIL QLY  +    D
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCA-GD 181

Query: 195 PSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQK 254
           P++ ILLL+  P+ +SLL++  +R I      D+ K L  L  VS+++A +++ + +++ 
Sbjct: 182 PASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKN 241

Query: 255 QVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVT 314
               S          +L +LALPLLI  R +                    G +K    T
Sbjct: 242 TFGLSSWANIVTLVCLLVMLALPLLIARRAQR------------------DGMEK----T 301

Query: 315 VPQNTPPTSTLQEITEISPSCLENICNKPERG--EDFTILQAIFSVDMALVYLATFTGCG 374
           VP +  P  +  + T       E   +K E G  E+  +LQA+  +   L++LA   G G
Sbjct: 302 VPHDYSPLISSPKATTSGNQSSEG-DSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMG 361

Query: 375 SSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMF 434
           S L++I+NI QIGESL Y +  I   VS  S++NF GR  +G+ S+ L+ K   PRPL+ 
Sbjct: 362 SGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLM 421

Query: 435 AFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCG 494
           A +     IG L IA    G++YV S+I+G  +G+Q  L+  I SELFG++   T+FN  
Sbjct: 422 AATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTI 481

Query: 495 QLVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGAL 554
            +  P GSYI +V ++G +YD  A       +G+G TC G+HCF  SF I+ASV  FG L
Sbjct: 482 SVASPIGSYIFSVRLIGYIYDKTA-------SGEGNTCYGSHCFRLSFIIMASVAFFGFL 529

Query: 555 VALVLAYRTREFYKGDVYKK 557
           VA+VL +RT+  Y+  + K+
Sbjct: 542 VAIVLFFRTKTLYRQILVKR 529

[Close]

BLAST of MC03g0325 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.6e-77
Identity = 191/585 (32.65%), Postives = 308/585 (52.65%), Query Frame = 0


Query: 13  IRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVG 72
           ++     RW    A+  I   AG  Y+FG+ S  IK+  NY+Q +++ LG AKDLG +VG
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 73  VFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFA 132
             AG L+E+ PLW   +VGS  N   Y  +WL VT R      W M I I +  N + + 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 133 NTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSF 192
           NTA LV+ V+NFP  RG ++G+LKGF G+GGAIL+Q+Y  ++   D ++LI +++  PS 
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHS-SDRASLIFMVAVAPSV 188

Query: 193 ISLLLLLAIRPI----KIR-KHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGY 252
           + + L+  IRP+    +IR        V+Y    V ILLA +++ + L++  +  SH+  
Sbjct: 189 VVVPLMFFIRPVGGHRQIRSSDATSFTVIY---AVCILLAAYLMAVMLVEDFIDLSHSII 248

Query: 253 ASGAFAILALLALPLLITVR--------------EELMLYKLNKQ--------NNDPSVI 312
            +    + A+L +P+ I +               EE +L     Q        ++ P +I
Sbjct: 249 IAFTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELI 308

Query: 313 VSIVGEQKLPMVTVPQNTPPTSTLQEITEISPSCLENIC---------NKPERGEDFTIL 372
            S V ++K   V +    P     + I ++    ++              P RGEDFT+ 
Sbjct: 309 FSEVEDEKPKEVDL---LPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLT 368

Query: 373 QAIFSVDMALVYLATFTGCGSSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVF 432
           QA+   D  L++ +   G GS L  IDN+GQ+ +SL Y   +  +FVS +S++NF GR+ 
Sbjct: 369 QALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVFVSMISIWNFLGRIG 428

Query: 433 SGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLL 492
            G+ SE ++  Y  PRP+  A + L+  +G +F A+  PG++++ +L+IG G+GA   ++
Sbjct: 429 GGYFSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIV 488

Query: 493 FAIISELFGLKRYSTLFNCGQLVVPFGSYILNVDIVGKLYDIEAIKEGSK---KTGKGLT 552
            A  SELFGLK++  L+N   L  P GS + +  I   +YD EA ++           L 
Sbjct: 489 PATASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLR 548

Query: 553 CTGAHCFGWSFTILASVTLFGALVALVLAYRTREFYKGDVYKKYR 559
           C G+ C+  +  I++   L  A ++++L  RT+  Y  ++Y K R
Sbjct: 549 CRGSICYFLTSLIMSGFCLIAAALSMILVQRTKPVYT-NLYGKTR 583

The following BLAST results are available for this feature:

BLAST of MC03g0325 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	1.4e-27	25.60	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of MC03g0325 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152846.1	0.0	100.00	uncharacterized protein LOC111020470 [Momordica charantia]	[more]
XP_022152849.1	8.59e-308	74.52	uncharacterized protein LOC111020473 [Momordica charantia]	[more]
XP_022970365.1	4.53e-305	71.53	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote...	[more]
XP_022964774.1	9.13e-305	71.53	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]
KAG6583526.1	9.13e-305	71.53	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of MC03g0325 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIZ3	0.0	100.00	uncharacterized protein LOC111020470 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1DHB0	4.16e-308	74.52	uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1I5D7	2.19e-305	71.53	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]
A0A6J1HP70	4.42e-305	71.53	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLT1	5.13e-304	71.18	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of MC03g0325 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	3.3e-168	53.41	Major facilitator superfamily protein	[more]
AT2G39210.1	3.2e-155	50.89	Major facilitator superfamily protein	[more]
AT5G14120.1	3.4e-80	32.80	Major facilitator superfamily protein	[more]
AT1G74780.1	2.2e-79	36.43	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT3G01930.2	1.6e-77	32.65	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 20..265 e-value: 8.6E-79 score: 264.5
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 321..550 e-value: 5.3E-13 score: 50.8 coord: 14..210 e-value: 1.1E-6 score: 30.2
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 6..209
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 250..549
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 5..560
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 5..560
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 19..535 e-value: 2.85439E-95 score: 294.544

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0325.1	MC03g0325.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity