MC03g0280 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0280
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionF-box/kelch-repeat protein
LocationMC03: 8322474 .. 8323517 (-)
RNA-Seq ExpressionMC03g0280
SyntenyMC03g0280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTGATTCACTGTTCCCAGGTCTCATTGATGATGTGGCCCTGAATTGCCTTGCTTGGGTTTGCCAATCAGATTACACTTCATTGTCATGTATTAACTCAAAGTTTAACAAGTTGGTGAGAAGTGGTGATCTATATGGGTGGAGGAAGCATTTGGGGATAAAGGAGCATTGGGTGTATCTGGTTTGTGATTTAAAGGGTTGGGAAGCATTTGATCCTTTGAGGAAAGTGTGGATGACTTTGCCTAAGATGCCTTGTGATGAGTGCTTCAATCATGCAGACAAGGAATCGTTGGCTGTAGGAAGCGAACTGTTAGTTTTTGGCCGTGAGATGTTTGACTTTGCTATATGGAAGTATAATTCGACTTGTAATAGTTGGGTAAAGTGTCAAGGAATGAACCGACGTCGCTGTCTGTTTGGCTCTGGTAGCCTTGGTCCGATTGCTATTGTAGCAGGTGGAAGTGATATAAATGGGAATGTTTTAGACTCTGCAGAGTTGTATGACTCTTCATTGGGTACATGGGAAATGTTGCCCAAGATGACTACCCCGCGTAGATTGTGTTCGGGTTTTTTTATGGATGGGAAGTTTTACGTGATTGGTGGAATGTCGAGCTCCACTGTTTCACTAACATGTGGGGAGGAGTACAATTTTCAAACAAGAAAATGGAGGAAAATTGAGGGAATGTATCCCTATGTCAATAGGGCTGCTCAGGCTCCTCCCCTTGTTGCTGTGGTGGATAACCAGTTATATGCTGTGGAGTATCTAACCAACCTGGTTAAGAGGTATGAAAAAGTGAAGAACACCTGGAATGTATTCGGAAGGCTTCCTCTGAGGGCGGATTCTTCGAATGGCTGGGGCCTGGCTTTCAAGACCTGTGGGGAGGAACTTCTGGTTGTAGGTGGACAAAGAGGTCCAAACGGTGAAGCAATCGTACTGAATGCTTGTTCTCCGAAGTTTGGCATGAGGAATGGCACCTTGGATTGGAAGTTTCTCGGTGTGAAGGAGCACGTCGGAGTGTTTGTCTATAACTGTGCTGTTATGGGTTGC

mRNA sequence

AGTGATTCACTGTTCCCAGGTCTCATTGATGATGTGGCCCTGAATTGCCTTGCTTGGGTTTGCCAATCAGATTACACTTCATTGTCATGTATTAACTCAAAGTTTAACAAGTTGGTGAGAAGTGGTGATCTATATGGGTGGAGGAAGCATTTGGGGATAAAGGAGCATTGGGTGTATCTGGTTTGTGATTTAAAGGGTTGGGAAGCATTTGATCCTTTGAGGAAAGTGTGGATGACTTTGCCTAAGATGCCTTGTGATGAGTGCTTCAATCATGCAGACAAGGAATCGTTGGCTGTAGGAAGCGAACTGTTAGTTTTTGGCCGTGAGATGTTTGACTTTGCTATATGGAAGTATAATTCGACTTGTAATAGTTGGGTAAAGTGTCAAGGAATGAACCGACGTCGCTGTCTGTTTGGCTCTGGTAGCCTTGGTCCGATTGCTATTGTAGCAGGTGGAAGTGATATAAATGGGAATGTTTTAGACTCTGCAGAGTTGTATGACTCTTCATTGGGTACATGGGAAATGTTGCCCAAGATGACTACCCCGCGTAGATTGTGTTCGGGTTTTTTTATGGATGGGAAGTTTTACGTGATTGGTGGAATGTCGAGCTCCACTGTTTCACTAACATGTGGGGAGGAGTACAATTTTCAAACAAGAAAATGGAGGAAAATTGAGGGAATGTATCCCTATGTCAATAGGGCTGCTCAGGCTCCTCCCCTTGTTGCTGTGGTGGATAACCAGTTATATGCTGTGGAGTATCTAACCAACCTGGTTAAGAGGTATGAAAAAGTGAAGAACACCTGGAATGTATTCGGAAGGCTTCCTCTGAGGGCGGATTCTTCGAATGGCTGGGGCCTGGCTTTCAAGACCTGTGGGGAGGAACTTCTGGTTGTAGGTGGACAAAGAGGTCCAAACGGTGAAGCAATCGTACTGAATGCTTGTTCTCCGAAGTTTGGCATGAGGAATGGCACCTTGGATTGGAAGTTTCTCGGTGTGAAGGAGCACGTCGGAGTGTTTGTCTATAACTGTGCTGTTATGGGTTGC

Coding sequence (CDS)

AGTGATTCACTGTTCCCAGGTCTCATTGATGATGTGGCCCTGAATTGCCTTGCTTGGGTTTGCCAATCAGATTACACTTCATTGTCATGTATTAACTCAAAGTTTAACAAGTTGGTGAGAAGTGGTGATCTATATGGGTGGAGGAAGCATTTGGGGATAAAGGAGCATTGGGTGTATCTGGTTTGTGATTTAAAGGGTTGGGAAGCATTTGATCCTTTGAGGAAAGTGTGGATGACTTTGCCTAAGATGCCTTGTGATGAGTGCTTCAATCATGCAGACAAGGAATCGTTGGCTGTAGGAAGCGAACTGTTAGTTTTTGGCCGTGAGATGTTTGACTTTGCTATATGGAAGTATAATTCGACTTGTAATAGTTGGGTAAAGTGTCAAGGAATGAACCGACGTCGCTGTCTGTTTGGCTCTGGTAGCCTTGGTCCGATTGCTATTGTAGCAGGTGGAAGTGATATAAATGGGAATGTTTTAGACTCTGCAGAGTTGTATGACTCTTCATTGGGTACATGGGAAATGTTGCCCAAGATGACTACCCCGCGTAGATTGTGTTCGGGTTTTTTTATGGATGGGAAGTTTTACGTGATTGGTGGAATGTCGAGCTCCACTGTTTCACTAACATGTGGGGAGGAGTACAATTTTCAAACAAGAAAATGGAGGAAAATTGAGGGAATGTATCCCTATGTCAATAGGGCTGCTCAGGCTCCTCCCCTTGTTGCTGTGGTGGATAACCAGTTATATGCTGTGGAGTATCTAACCAACCTGGTTAAGAGGTATGAAAAAGTGAAGAACACCTGGAATGTATTCGGAAGGCTTCCTCTGAGGGCGGATTCTTCGAATGGCTGGGGCCTGGCTTTCAAGACCTGTGGGGAGGAACTTCTGGTTGTAGGTGGACAAAGAGGTCCAAACGGTGAAGCAATCGTACTGAATGCTTGTTCTCCGAAGTTTGGCATGAGGAATGGCACCTTGGATTGGAAGTTTCTCGGTGTGAAGGAGCACGTCGGAGTGTTTGTCTATAACTGTGCTGTTATGGGTTGC

Protein sequence

SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYLVCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNSTCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMTTPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC
Homology
BLAST of MC03g0280 vs. ExPASy Swiss-Prot
Match: Q9FKJ0 (F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana OX=3702 GN=At5g60570 PE=2 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 1.7e-156
Identity = 252/348 (72.41%), Postives = 291/348 (83.62%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDS+ PGLIDDVALNCLAWV +SDY SLSC+N K+NKL+ SG L+  RK LGI E+ V++
Sbjct: 46  SDSVLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVEYLVFM 105

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCD +GW  F P++K WM LPKMPCDECFNHADKESLAV  ELLVFGRE+F FAIWKY+ 
Sbjct: 106 VCDPRGWLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDELLVFGRELFQFAIWKYSL 165

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
               WVKC+GM+R RCLF SGSLG IAIVAGG+D+NGN+L SAELYDSS G WEMLP M 
Sbjct: 166 RSRCWVKCEGMHRPRCLFASGSLGGIAIVAGGTDMNGNILASAELYDSSSGRWEMLPNMH 225

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           +PRRLCSGFFMDGKFYVIGGMSS  VS+T GEE++ +TRKWRKIEGMYP VNRAAQAPPL
Sbjct: 226 SPRRLCSGFFMDGKFYVIGGMSSPNVSVTFGEEFDLETRKWRKIEGMYPNVNRAAQAPPL 285

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           V VV+N+L+ +EY TN+VK+Y+KVKN W V GRLP   DSSNGWGLAFK CG++LLV  G
Sbjct: 286 VVVVNNELFTLEYSTNMVKKYDKVKNKWEVMGRLPPMVDSSNGWGLAFKPCGDQLLVFCG 345

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           QRGP+GE IV+N+  PK G ++G LDWK LGVKE+VGVFVYNCAVMGC
Sbjct: 346 QRGPHGEGIVVNSWCPKSGAKDGNLDWKVLGVKENVGVFVYNCAVMGC 393

BLAST of MC03g0280 vs. ExPASy Swiss-Prot
Match: Q84M94 (F-box/kelch-repeat protein At1g26930 OS=Arabidopsis thaliana OX=3702 GN=At1g26930 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.7e-98
Identity = 176/359 (49.03%), Postives = 232/359 (64.62%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           S +L PG+  D +L+CL    ++DY S++ +N     L+RSG++Y  R+  G  EHWVY 
Sbjct: 68  SGTLIPGMNRDDSLSCLIRCSRADYCSIASVNRSLRSLIRSGEIYRLRRLQGTLEHWVYF 127

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
            C L  WEAFDP  K WM LP MP +ECF +ADKESLAVG++LLVFG E+  + I++Y+ 
Sbjct: 128 SCHLNEWEAFDPRSKRWMHLPSMPQNECFRYADKESLAVGTDLLVFGWEVSSYVIYRYSL 187

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
             NSW   + MN  RCLFGS S G IA++AGG D +G +LD+AELY+    TW +LP M 
Sbjct: 188 LTNSWSTAKSMNMPRCLFGSASYGEIAVLAGGCDSSGRILDTAELYNYEDQTWLVLPGMN 247

Query: 181 TPRRLCSGFFMDGKFYVIGGM----SSSTVSLTCGEEYNFQTRKWRKIEGMYP------- 240
             R++CSG FMDGKFYVIGG+     +    LTCGEE++ +TRKW +I  M P       
Sbjct: 248 KRRKMCSGVFMDGKFYVIGGIGVGEENEPKVLTCGEEFDLKTRKWTEIPEMSPPRSNQGN 307

Query: 241 YVNRAAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFK 300
            ++ AA APPLVAVV++QLYA ++    V+RY+K K  WN  G LP +A S NGWGLAF+
Sbjct: 308 GMSAAAMAPPLVAVVNDQLYAADHAGMAVRRYDKEKRVWNKVGNLPEQAGSMNGWGLAFR 367

Query: 301 TCGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
            CG+ ++V+GG + P    I LN+  P       T +W  LG K+ V  FVYNCAVM C
Sbjct: 368 ACGDRIIVIGGPKAPGEGFIELNSWVPSV----TTPEWHLLGKKQSVN-FVYNCAVMSC 421

BLAST of MC03g0280 vs. ExPASy Swiss-Prot
Match: Q8L736 (F-box/kelch-repeat protein SKIP11 OS=Arabidopsis thaliana OX=3702 GN=SKIP11 PE=1 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 3.7e-95
Identity = 177/358 (49.44%), Postives = 227/358 (63.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           S SL   +  D +++CL    +SDY S++ +N  F  LV+SG++Y  R+  G  EHWVY 
Sbjct: 114 SHSLINEIGRDNSIDCLIRCSRSDYGSIASLNRNFRSLVKSGEIYRLRRQNGFVEHWVYF 173

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMF-DFAIWKYN 120
            C L  W AFDP+ + WM LP MP    F  ADKESLAVG++LLV G++ F    I++Y+
Sbjct: 174 SCQLLEWVAFDPVERRWMQLPTMPSSVTFMCADKESLAVGTDLLVLGKDDFSSHVIYRYS 233

Query: 121 STCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKM 180
              NSW     MN  RCLFGS SLG IAI AGG D  G +LD AE+Y+S L TW  LP+M
Sbjct: 234 LLTNSWSSGMKMNSPRCLFGSASLGEIAIFAGGCDSQGKILDFAEMYNSELQTWITLPRM 293

Query: 181 TTPRRLCSGFFMDGKFYVIGGM-SSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNR----- 240
             PR++CSG FMDGKFYVIGG+  + +  LTCGEEY+ +T+KW +I  + P  +R     
Sbjct: 294 NKPRKMCSGVFMDGKFYVIGGIGGADSKGLTCGEEYDLETKKWTQIPDLSPPRSRADQAD 353

Query: 241 ---AAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKT 300
              AA+APPLVAVV+NQLYA ++    V++Y+K    W   GRLP RA S NGWGLAF+ 
Sbjct: 354 MSPAAEAPPLVAVVNNQLYAADHADMEVRKYDKENKKWLTVGRLPERAGSVNGWGLAFRA 413

Query: 301 CGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           CGE L+V+GG +   G  I LN+  P  G   G   W  L  ++H   FVYNCAVMGC
Sbjct: 414 CGERLIVIGGPKCSGGGFIELNSWIPSDG---GPPQWTLLD-RKHSPTFVYNCAVMGC 467

BLAST of MC03g0280 vs. ExPASy Swiss-Prot
Match: Q9CA63 (F-box/kelch-repeat protein At1g74510 OS=Arabidopsis thaliana OX=3702 GN=At1g74510 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.2e-91
Identity = 166/357 (46.50%), Postives = 215/357 (60.22%), Query Frame = 0

Query: 8   LIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYLVCDLKGW 67
           L  +  LNCLA    SD+ S++  N  F  L++  +LY  R+  GI EHW+Y  C L  W
Sbjct: 99  LDQNALLNCLAHCSLSDFGSIASTNRTFRSLIKDSELYRLRRAKGIVEHWIYFSCRLLEW 158

Query: 68  EAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNSTCNSWVK 127
           EA+DP    W+ +PKM  +ECF  +DKESLAVG+ELLVFG+E+    I++Y+   N+W  
Sbjct: 159 EAYDPNGDRWLRVPKMTFNECFMCSDKESLAVGTELLVFGKEIMSHVIYRYSILTNTWTS 218

Query: 128 CQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMTTPRRLCS 187
              MN  RCLFGS SLG IA++AGG D  G +L SAELY+S  G W ++P M   R++CS
Sbjct: 219 GMQMNVPRCLFGSASLGEIAVIAGGCDPRGRILSSAELYNSETGEWTVIPSMNKARKMCS 278

Query: 188 GFFMDGKFYVIGGM-SSSTVSLTCGEEYNFQTRKWRKIEGMYP---------------YV 247
             FMDG FY IGG+   ++  L CGE Y+ + + W  I  M P                 
Sbjct: 279 SVFMDGNFYCIGGIGEGNSKMLLCGEVYDLKKKTWTLIPNMLPERSSGGGGDQAKEIAAA 338

Query: 248 NRAAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTC 307
             A++APPLVAVV ++LYA  Y    VK+Y+K  N WN  G LP RA S NGWG+AF+ C
Sbjct: 339 TAASEAPPLVAVVKDELYAANYAQQEVKKYDKRLNVWNKVGNLPERASSMNGWGMAFRAC 398

Query: 308 GEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           G++L+VVGG R   G  I +NAC P  G +   L W+ L  K   G FVYNCAVMGC
Sbjct: 399 GDQLVVVGGPRAIGGGFIEINACVPSEGTQ---LHWRVLASKPS-GNFVYNCAVMGC 451

BLAST of MC03g0280 vs. ExPASy Swiss-Prot
Match: Q9LI89 (F-box/kelch-repeat protein At3g27150 OS=Arabidopsis thaliana OX=3702 GN=At3g27150 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.8e-61
Identity = 129/352 (36.65%), Postives = 194/352 (55.11%), Query Frame = 0

Query: 6   PGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYLVCDLK 65
           P L+ ++ +  LA V + +Y  L  +N  F++L++S +++  R+  G+ E  V+++    
Sbjct: 72  PQLVYELEVEILARVPRFEYWKLKLLNKGFSRLLKSDEIFKVRRERGVVEPSVFMLSSGD 131

Query: 66  G-WEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNSTCNS 125
             W  FD        LP++P D CF H DKESL  G+ L+V G+E    A+W+Y    + 
Sbjct: 132 TCWTMFDKGFGNCQKLPELPSDICFLHGDKESLCAGTHLIVTGKEEKSIALWRYELETSK 191

Query: 126 WVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGN----VLDSAELYDSSLGTWEMLPKMT 185
           W K   M   R LF S + G +  VAGG  I GN    V+DS E YDS   TW +L  M 
Sbjct: 192 WFKGPAMITPRILFASATCGTVVFVAGGLKIEGNGTMEVVDSVEKYDSKTKTWTLLRGMH 251

Query: 186 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVN-RAAQAPP 245
             R+ CSG ++ GKFYV+GG   +  +LTCGE Y+ +T  W  I  +   ++  + Q+PP
Sbjct: 252 KRRKFCSGCYLRGKFYVLGGRDENGQNLTCGESYDEKTNTWELIPDILKDMSFSSVQSPP 311

Query: 246 LVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVG 305
           L+AVV + LY++E   N ++ Y+   N+W   G +P+RA S+ GWG+AFK+ G++LLV+G
Sbjct: 312 LIAVVGDDLYSLETSANELRVYDANANSWKKLGDVPVRAKSNGGWGVAFKSLGDKLLVIG 371

Query: 306 GQRGPN-GEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGV----FVYNCAVM 347
              GP+  E + +    P     N  L W+    K   GV    F+ NC VM
Sbjct: 372 ASAGPSRAETMSVYTSRPSANPAN-KLYWE--ESKRCCGVRFNHFILNCCVM 420

BLAST of MC03g0280 vs. NCBI nr
Match: XP_022156954.1 (F-box/kelch-repeat protein At5g60570-like [Momordica charantia] >XP_022156955.1 F-box/kelch-repeat protein At5g60570-like [Momordica charantia])

HSP 1 Score: 738 bits (1906), Expect = 8.60e-269
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL
Sbjct: 43  SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 102

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 103 VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 162

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT
Sbjct: 163 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 222

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 223 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 282

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG
Sbjct: 283 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 342

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 343 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 390

BLAST of MC03g0280 vs. NCBI nr
Match: XP_011653482.1 (F-box/kelch-repeat protein At5g60570 [Cucumis sativus] >KGN64735.1 hypothetical protein Csa_013744 [Cucumis sativus])

HSP 1 Score: 708 bits (1828), Expect = 6.42e-257
Identity = 329/348 (94.54%), Postives = 340/348 (97.70%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYT+LSC+NS+FNKLVR+GDLY WRKHLGIKEHWVYL
Sbjct: 42  SDSLFPGLIDDVALNCLAWTCQSDYTALSCLNSRFNKLVRNGDLYEWRKHLGIKEHWVYL 101

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVG+ELLVFGREMFDFAIWKYNS
Sbjct: 102 VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGTELLVFGREMFDFAIWKYNS 161

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KCQGMNR RCLFGSGSLG IAIVAGGSD+NGNVLDSAELYDSSLGTWEMLPKMT
Sbjct: 162 TCNSWAKCQGMNRPRCLFGSGSLGSIAIVAGGSDMNGNVLDSAELYDSSLGTWEMLPKMT 221

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMDGKF+VIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 222 TPRRLCSGFFMDGKFFVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 281

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLYAVEYLTNLVKRY+K+KNTWNV GRLPLRADSSNGWGLAFK CGEELLV+GG
Sbjct: 282 VAVVDNQLYAVEYLTNLVKRYDKIKNTWNVLGRLPLRADSSNGWGLAFKACGEELLVIGG 341

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 342 QKGPNGEAIVLNACSPKFGMRNGGLDWKFLGVKEHVGVFVYNCAVMGC 389

BLAST of MC03g0280 vs. NCBI nr
Match: XP_023521249.1 (F-box/kelch-repeat protein At5g60570-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706 bits (1822), Expect = 5.08e-256
Identity = 329/348 (94.54%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYTSLSC+NS+FN+LVR+GDLYGWRK LGIKEHWVYL
Sbjct: 41  SDSLFPGLIDDVALNCLAWACQSDYTSLSCLNSRFNELVRNGDLYGWRKRLGIKEHWVYL 100

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEA+DPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 101 VCDLKGWEAYDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 160

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KCQGMNR RCLFGSGSLG IAIVAGGSDINGN+LDSAELYDSSLGTWEMLPKMT
Sbjct: 161 TCNSWTKCQGMNRPRCLFGSGSLGSIAIVAGGSDINGNILDSAELYDSSLGTWEMLPKMT 220

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMD KFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 221 TPRRLCSGFFMDEKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 280

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLY+VEYLTNLVKRY+KVKN+WNV GRLPLRADSSNGWGLAFK CGEELLVVGG
Sbjct: 281 VAVVDNQLYSVEYLTNLVKRYDKVKNSWNVLGRLPLRADSSNGWGLAFKACGEELLVVGG 340

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 341 QKGPNGEAIVLNACSPKFGMRNGALDWKFLGVKEHVGVFVYNCAVMGC 388

BLAST of MC03g0280 vs. NCBI nr
Match: XP_022964959.1 (F-box/kelch-repeat protein At5g60570-like [Cucurbita moschata] >XP_022964960.1 F-box/kelch-repeat protein At5g60570-like [Cucurbita moschata])

HSP 1 Score: 704 bits (1818), Expect = 2.59e-255
Identity = 328/348 (94.25%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYTSLSC+NS+FN+LVR+GDLYGWRK LGIKEHWVYL
Sbjct: 47  SDSLFPGLIDDVALNCLAWACQSDYTSLSCLNSRFNELVRNGDLYGWRKRLGIKEHWVYL 106

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEA+DPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 107 VCDLKGWEAYDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 166

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KC+GMNR RCLFGSGSLG IAIVAGGSDINGN+LDSAELYDSSLGTWEMLPKMT
Sbjct: 167 TCNSWTKCRGMNRPRCLFGSGSLGSIAIVAGGSDINGNILDSAELYDSSLGTWEMLPKMT 226

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMD KFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 227 TPRRLCSGFFMDEKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 286

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLY+VEYLTNLVKRY+KVKN+WNV GRLPLRADSSNGWGLAFK CGEELLVVGG
Sbjct: 287 VAVVDNQLYSVEYLTNLVKRYDKVKNSWNVLGRLPLRADSSNGWGLAFKACGEELLVVGG 346

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 347 QKGPNGEAIVLNACSPKFGMRNGALDWKFLGVKEHVGVFVYNCAVMGC 394

BLAST of MC03g0280 vs. NCBI nr
Match: KAG6583486.1 (F-box/kelch-repeat protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 703 bits (1815), Expect = 4.07e-255
Identity = 327/348 (93.97%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYTSLSC+NS+FN+LV++GDLYGWRK LGIKEHWVYL
Sbjct: 31  SDSLFPGLIDDVALNCLAWACQSDYTSLSCLNSRFNELVKNGDLYGWRKRLGIKEHWVYL 90

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEA+DPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 91  VCDLKGWEAYDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 150

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KC+GMNR RCLFGSGSLG IAIVAGGSDINGN+LDSAELYDSSLGTWEMLPKMT
Sbjct: 151 TCNSWTKCRGMNRPRCLFGSGSLGSIAIVAGGSDINGNILDSAELYDSSLGTWEMLPKMT 210

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMD KFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 211 TPRRLCSGFFMDEKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 270

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLY+VEYLTNLVKRY+KVKN+WNV GRLPLRADSSNGWGLAFK CGEELLVVGG
Sbjct: 271 VAVVDNQLYSVEYLTNLVKRYDKVKNSWNVLGRLPLRADSSNGWGLAFKACGEELLVVGG 330

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 331 QKGPNGEAIVLNACSPKFGMRNGALDWKFLGVKEHVGVFVYNCAVMGC 378

BLAST of MC03g0280 vs. ExPASy TrEMBL
Match: A0A6J1DV43 (F-box/kelch-repeat protein At5g60570-like OS=Momordica charantia OX=3673 GN=LOC111023783 PE=4 SV=1)

HSP 1 Score: 738 bits (1906), Expect = 4.17e-269
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL
Sbjct: 43  SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 102

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 103 VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 162

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT
Sbjct: 163 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 222

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 223 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 282

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG
Sbjct: 283 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 342

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 343 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 390

BLAST of MC03g0280 vs. ExPASy TrEMBL
Match: A0A0A0LS32 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G084830 PE=4 SV=1)

HSP 1 Score: 708 bits (1828), Expect = 3.11e-257
Identity = 329/348 (94.54%), Postives = 340/348 (97.70%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYT+LSC+NS+FNKLVR+GDLY WRKHLGIKEHWVYL
Sbjct: 42  SDSLFPGLIDDVALNCLAWTCQSDYTALSCLNSRFNKLVRNGDLYEWRKHLGIKEHWVYL 101

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVG+ELLVFGREMFDFAIWKYNS
Sbjct: 102 VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGTELLVFGREMFDFAIWKYNS 161

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KCQGMNR RCLFGSGSLG IAIVAGGSD+NGNVLDSAELYDSSLGTWEMLPKMT
Sbjct: 162 TCNSWAKCQGMNRPRCLFGSGSLGSIAIVAGGSDMNGNVLDSAELYDSSLGTWEMLPKMT 221

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMDGKF+VIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 222 TPRRLCSGFFMDGKFFVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 281

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLYAVEYLTNLVKRY+K+KNTWNV GRLPLRADSSNGWGLAFK CGEELLV+GG
Sbjct: 282 VAVVDNQLYAVEYLTNLVKRYDKIKNTWNVLGRLPLRADSSNGWGLAFKACGEELLVIGG 341

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 342 QKGPNGEAIVLNACSPKFGMRNGGLDWKFLGVKEHVGVFVYNCAVMGC 389

BLAST of MC03g0280 vs. ExPASy TrEMBL
Match: A0A6J1HPP5 (F-box/kelch-repeat protein At5g60570-like OS=Cucurbita moschata OX=3662 GN=LOC111464911 PE=4 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 1.25e-255
Identity = 328/348 (94.25%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYTSLSC+NS+FN+LVR+GDLYGWRK LGIKEHWVYL
Sbjct: 47  SDSLFPGLIDDVALNCLAWACQSDYTSLSCLNSRFNELVRNGDLYGWRKRLGIKEHWVYL 106

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEA+DPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 107 VCDLKGWEAYDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 166

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KC+GMNR RCLFGSGSLG IAIVAGGSDINGN+LDSAELYDSSLGTWEMLPKMT
Sbjct: 167 TCNSWTKCRGMNRPRCLFGSGSLGSIAIVAGGSDINGNILDSAELYDSSLGTWEMLPKMT 226

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMD KFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 227 TPRRLCSGFFMDEKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 286

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLY+VEYLTNLVKRY+KVKN+WNV GRLPLRADSSNGWGLAFK CGEELLVVGG
Sbjct: 287 VAVVDNQLYSVEYLTNLVKRYDKVKNSWNVLGRLPLRADSSNGWGLAFKACGEELLVVGG 346

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 347 QKGPNGEAIVLNACSPKFGMRNGALDWKFLGVKEHVGVFVYNCAVMGC 394

BLAST of MC03g0280 vs. ExPASy TrEMBL
Match: A0A5D3BCP0 (F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold690G00220 PE=4 SV=1)

HSP 1 Score: 703 bits (1815), Expect = 2.21e-255
Identity = 327/348 (93.97%), Postives = 338/348 (97.13%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYT+LSC+NS+FNKLVR+GDLY WRKHLGIKEHWVYL
Sbjct: 34  SDSLFPGLIDDVALNCLAWACQSDYTALSCLNSRFNKLVRNGDLYEWRKHLGIKEHWVYL 93

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVG+ELLVFGREMFDFAIWKYNS
Sbjct: 94  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGTELLVFGREMFDFAIWKYNS 153

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KC GMNR RCLFGSGSLG IAIVAGGSD+NGNVLDSAELYDSSLGTWEMLPKMT
Sbjct: 154 TCNSWAKCPGMNRPRCLFGSGSLGSIAIVAGGSDMNGNVLDSAELYDSSLGTWEMLPKMT 213

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMDGKF+VIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 214 TPRRLCSGFFMDGKFFVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 273

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLYAVEYLTNLVKRY+K+KNTWNV GRLPLRADSSNGWGLAFK CG ELLV+GG
Sbjct: 274 VAVVDNQLYAVEYLTNLVKRYDKIKNTWNVLGRLPLRADSSNGWGLAFKACGGELLVIGG 333

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 334 QKGPNGEAIVLNACSPKFGMRNGGLDWKFLGVKEHVGVFVYNCAVMGC 381

BLAST of MC03g0280 vs. ExPASy TrEMBL
Match: A0A6J1I4G4 (F-box/kelch-repeat protein At5g60570-like OS=Cucurbita maxima OX=3661 GN=LOC111469560 PE=4 SV=1)

HSP 1 Score: 704 bits (1816), Expect = 2.53e-255
Identity = 327/348 (93.97%), Postives = 339/348 (97.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDSLFPGLIDDVALNCLAW CQSDYTSLSC+NS+FN+LVR+GDLYGWRKHLGIKEHWVYL
Sbjct: 47  SDSLFPGLIDDVALNCLAWACQSDYTSLSCLNSRFNELVRNGDLYGWRKHLGIKEHWVYL 106

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCDLKGWEA+DPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS
Sbjct: 107 VCDLKGWEAYDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 166

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
           TCNSW KC+GMN+ RCLFGSGSLG IAIVAGGSDINGN+LDSAELYDSSLGTWEMLPKM 
Sbjct: 167 TCNSWTKCRGMNQPRCLFGSGSLGSIAIVAGGSDINGNILDSAELYDSSLGTWEMLPKMM 226

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           TPRRLCSGFFMD KFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL
Sbjct: 227 TPRRLCSGFFMDEKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 286

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           VAVVDNQLY+VEYLTNLVKRY+KVKN+WNV GRLPLRADSSNGWGLAFK CGEELLVVGG
Sbjct: 287 VAVVDNQLYSVEYLTNLVKRYDKVKNSWNVLGRLPLRADSSNGWGLAFKACGEELLVVGG 346

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 348
           Q+GPNGEAIVLNACSPKFGMRNG LDWKFLGVKEHVGVFVYNCAVMGC
Sbjct: 347 QKGPNGEAIVLNACSPKFGMRNGALDWKFLGVKEHVGVFVYNCAVMGC 394

BLAST of MC03g0280 vs. TAIR 10
Match: AT5G60570.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 553.5 bits (1425), Expect = 1.2e-157
Identity = 252/348 (72.41%), Postives = 291/348 (83.62%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           SDS+ PGLIDDVALNCLAWV +SDY SLSC+N K+NKL+ SG L+  RK LGI E+ V++
Sbjct: 46  SDSVLPGLIDDVALNCLAWVPRSDYPSLSCVNKKYNKLINSGHLFALRKELGIVEYLVFM 105

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
           VCD +GW  F P++K WM LPKMPCDECFNHADKESLAV  ELLVFGRE+F FAIWKY+ 
Sbjct: 106 VCDPRGWLMFSPMKKKWMVLPKMPCDECFNHADKESLAVDDELLVFGRELFQFAIWKYSL 165

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
               WVKC+GM+R RCLF SGSLG IAIVAGG+D+NGN+L SAELYDSS G WEMLP M 
Sbjct: 166 RSRCWVKCEGMHRPRCLFASGSLGGIAIVAGGTDMNGNILASAELYDSSSGRWEMLPNMH 225

Query: 181 TPRRLCSGFFMDGKFYVIGGMSSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNRAAQAPPL 240
           +PRRLCSGFFMDGKFYVIGGMSS  VS+T GEE++ +TRKWRKIEGMYP VNRAAQAPPL
Sbjct: 226 SPRRLCSGFFMDGKFYVIGGMSSPNVSVTFGEEFDLETRKWRKIEGMYPNVNRAAQAPPL 285

Query: 241 VAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKTCGEELLVVGG 300
           V VV+N+L+ +EY TN+VK+Y+KVKN W V GRLP   DSSNGWGLAFK CG++LLV  G
Sbjct: 286 VVVVNNELFTLEYSTNMVKKYDKVKNKWEVMGRLPPMVDSSNGWGLAFKPCGDQLLVFCG 345

Query: 301 QRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           QRGP+GE IV+N+  PK G ++G LDWK LGVKE+VGVFVYNCAVMGC
Sbjct: 346 QRGPHGEGIVVNSWCPKSGAKDGNLDWKVLGVKENVGVFVYNCAVMGC 393

BLAST of MC03g0280 vs. TAIR 10
Match: AT1G26930.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-99
Identity = 176/359 (49.03%), Postives = 232/359 (64.62%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           S +L PG+  D +L+CL    ++DY S++ +N     L+RSG++Y  R+  G  EHWVY 
Sbjct: 68  SGTLIPGMNRDDSLSCLIRCSRADYCSIASVNRSLRSLIRSGEIYRLRRLQGTLEHWVYF 127

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMFDFAIWKYNS 120
            C L  WEAFDP  K WM LP MP +ECF +ADKESLAVG++LLVFG E+  + I++Y+ 
Sbjct: 128 SCHLNEWEAFDPRSKRWMHLPSMPQNECFRYADKESLAVGTDLLVFGWEVSSYVIYRYSL 187

Query: 121 TCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKMT 180
             NSW   + MN  RCLFGS S G IA++AGG D +G +LD+AELY+    TW +LP M 
Sbjct: 188 LTNSWSTAKSMNMPRCLFGSASYGEIAVLAGGCDSSGRILDTAELYNYEDQTWLVLPGMN 247

Query: 181 TPRRLCSGFFMDGKFYVIGGM----SSSTVSLTCGEEYNFQTRKWRKIEGMYP------- 240
             R++CSG FMDGKFYVIGG+     +    LTCGEE++ +TRKW +I  M P       
Sbjct: 248 KRRKMCSGVFMDGKFYVIGGIGVGEENEPKVLTCGEEFDLKTRKWTEIPEMSPPRSNQGN 307

Query: 241 YVNRAAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFK 300
            ++ AA APPLVAVV++QLYA ++    V+RY+K K  WN  G LP +A S NGWGLAF+
Sbjct: 308 GMSAAAMAPPLVAVVNDQLYAADHAGMAVRRYDKEKRVWNKVGNLPEQAGSMNGWGLAFR 367

Query: 301 TCGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
            CG+ ++V+GG + P    I LN+  P       T +W  LG K+ V  FVYNCAVM C
Sbjct: 368 ACGDRIIVIGGPKAPGEGFIELNSWVPSV----TTPEWHLLGKKQSVN-FVYNCAVMSC 421

BLAST of MC03g0280 vs. TAIR 10
Match: AT2G02870.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.6e-96
Identity = 177/358 (49.44%), Postives = 227/358 (63.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           S SL   +  D +++CL    +SDY S++ +N  F  LV+SG++Y  R+  G  EHWVY 
Sbjct: 114 SHSLINEIGRDNSIDCLIRCSRSDYGSIASLNRNFRSLVKSGEIYRLRRQNGFVEHWVYF 173

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMF-DFAIWKYN 120
            C L  W AFDP+ + WM LP MP    F  ADKESLAVG++LLV G++ F    I++Y+
Sbjct: 174 SCQLLEWVAFDPVERRWMQLPTMPSSVTFMCADKESLAVGTDLLVLGKDDFSSHVIYRYS 233

Query: 121 STCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKM 180
              NSW     MN  RCLFGS SLG IAI AGG D  G +LD AE+Y+S L TW  LP+M
Sbjct: 234 LLTNSWSSGMKMNSPRCLFGSASLGEIAIFAGGCDSQGKILDFAEMYNSELQTWITLPRM 293

Query: 181 TTPRRLCSGFFMDGKFYVIGGM-SSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNR----- 240
             PR++CSG FMDGKFYVIGG+  + +  LTCGEEY+ +T+KW +I  + P  +R     
Sbjct: 294 NKPRKMCSGVFMDGKFYVIGGIGGADSKGLTCGEEYDLETKKWTQIPDLSPPRSRADQAD 353

Query: 241 ---AAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKT 300
              AA+APPLVAVV+NQLYA ++    V++Y+K    W   GRLP RA S NGWGLAF+ 
Sbjct: 354 MSPAAEAPPLVAVVNNQLYAADHADMEVRKYDKENKKWLTVGRLPERAGSVNGWGLAFRA 413

Query: 301 CGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           CGE L+V+GG +   G  I LN+  P  G   G   W  L  ++H   FVYNCAVMGC
Sbjct: 414 CGERLIVIGGPKCSGGGFIELNSWIPSDG---GPPQWTLLD-RKHSPTFVYNCAVMGC 467

BLAST of MC03g0280 vs. TAIR 10
Match: AT2G02870.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.6e-96
Identity = 177/358 (49.44%), Postives = 227/358 (63.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           S SL   +  D +++CL    +SDY S++ +N  F  LV+SG++Y  R+  G  EHWVY 
Sbjct: 114 SHSLINEIGRDNSIDCLIRCSRSDYGSIASLNRNFRSLVKSGEIYRLRRQNGFVEHWVYF 173

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMF-DFAIWKYN 120
            C L  W AFDP+ + WM LP MP    F  ADKESLAVG++LLV G++ F    I++Y+
Sbjct: 174 SCQLLEWVAFDPVERRWMQLPTMPSSVTFMCADKESLAVGTDLLVLGKDDFSSHVIYRYS 233

Query: 121 STCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKM 180
              NSW     MN  RCLFGS SLG IAI AGG D  G +LD AE+Y+S L TW  LP+M
Sbjct: 234 LLTNSWSSGMKMNSPRCLFGSASLGEIAIFAGGCDSQGKILDFAEMYNSELQTWITLPRM 293

Query: 181 TTPRRLCSGFFMDGKFYVIGGM-SSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNR----- 240
             PR++CSG FMDGKFYVIGG+  + +  LTCGEEY+ +T+KW +I  + P  +R     
Sbjct: 294 NKPRKMCSGVFMDGKFYVIGGIGGADSKGLTCGEEYDLETKKWTQIPDLSPPRSRADQAD 353

Query: 241 ---AAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKT 300
              AA+APPLVAVV+NQLYA ++    V++Y+K    W   GRLP RA S NGWGLAF+ 
Sbjct: 354 MSPAAEAPPLVAVVNNQLYAADHADMEVRKYDKENKKWLTVGRLPERAGSVNGWGLAFRA 413

Query: 301 CGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           CGE L+V+GG +   G  I LN+  P  G   G   W  L  ++H   FVYNCAVMGC
Sbjct: 414 CGERLIVIGGPKCSGGGFIELNSWIPSDG---GPPQWTLLD-RKHSPTFVYNCAVMGC 467

BLAST of MC03g0280 vs. TAIR 10
Match: AT2G02870.3 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.6e-96
Identity = 177/358 (49.44%), Postives = 227/358 (63.41%), Query Frame = 0

Query: 1   SDSLFPGLIDDVALNCLAWVCQSDYTSLSCINSKFNKLVRSGDLYGWRKHLGIKEHWVYL 60
           S SL   +  D +++CL    +SDY S++ +N  F  LV+SG++Y  R+  G  EHWVY 
Sbjct: 114 SHSLINEIGRDNSIDCLIRCSRSDYGSIASLNRNFRSLVKSGEIYRLRRQNGFVEHWVYF 173

Query: 61  VCDLKGWEAFDPLRKVWMTLPKMPCDECFNHADKESLAVGSELLVFGREMF-DFAIWKYN 120
            C L  W AFDP+ + WM LP MP    F  ADKESLAVG++LLV G++ F    I++Y+
Sbjct: 174 SCQLLEWVAFDPVERRWMQLPTMPSSVTFMCADKESLAVGTDLLVLGKDDFSSHVIYRYS 233

Query: 121 STCNSWVKCQGMNRRRCLFGSGSLGPIAIVAGGSDINGNVLDSAELYDSSLGTWEMLPKM 180
              NSW     MN  RCLFGS SLG IAI AGG D  G +LD AE+Y+S L TW  LP+M
Sbjct: 234 LLTNSWSSGMKMNSPRCLFGSASLGEIAIFAGGCDSQGKILDFAEMYNSELQTWITLPRM 293

Query: 181 TTPRRLCSGFFMDGKFYVIGGM-SSSTVSLTCGEEYNFQTRKWRKIEGMYPYVNR----- 240
             PR++CSG FMDGKFYVIGG+  + +  LTCGEEY+ +T+KW +I  + P  +R     
Sbjct: 294 NKPRKMCSGVFMDGKFYVIGGIGGADSKGLTCGEEYDLETKKWTQIPDLSPPRSRADQAD 353

Query: 241 ---AAQAPPLVAVVDNQLYAVEYLTNLVKRYEKVKNTWNVFGRLPLRADSSNGWGLAFKT 300
              AA+APPLVAVV+NQLYA ++    V++Y+K    W   GRLP RA S NGWGLAF+ 
Sbjct: 354 MSPAAEAPPLVAVVNNQLYAADHADMEVRKYDKENKKWLTVGRLPERAGSVNGWGLAFRA 413

Query: 301 CGEELLVVGGQRGPNGEAIVLNACSPKFGMRNGTLDWKFLGVKEHVGVFVYNCAVMGC 349
           CGE L+V+GG +   G  I LN+  P  G   G   W  L  ++H   FVYNCAVMGC
Sbjct: 414 CGERLIVIGGPKCSGGGFIELNSWIPSDG---GPPQWTLLD-RKHSPTFVYNCAVMGC 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKJ01.7e-15672.41F-box/kelch-repeat protein At5g60570 OS=Arabidopsis thaliana OX=3702 GN=At5g6057... [more]
Q84M942.7e-9849.03F-box/kelch-repeat protein At1g26930 OS=Arabidopsis thaliana OX=3702 GN=At1g2693... [more]
Q8L7363.7e-9549.44F-box/kelch-repeat protein SKIP11 OS=Arabidopsis thaliana OX=3702 GN=SKIP11 PE=1... [more]
Q9CA634.2e-9146.50F-box/kelch-repeat protein At1g74510 OS=Arabidopsis thaliana OX=3702 GN=At1g7451... [more]
Q9LI897.8e-6136.65F-box/kelch-repeat protein At3g27150 OS=Arabidopsis thaliana OX=3702 GN=At3g2715... [more]
Match NameE-valueIdentityDescription
XP_022156954.18.60e-269100.00F-box/kelch-repeat protein At5g60570-like [Momordica charantia] >XP_022156955.1 ... [more]
XP_011653482.16.42e-25794.54F-box/kelch-repeat protein At5g60570 [Cucumis sativus] >KGN64735.1 hypothetical ... [more]
XP_023521249.15.08e-25694.54F-box/kelch-repeat protein At5g60570-like [Cucurbita pepo subsp. pepo][more]
XP_022964959.12.59e-25594.25F-box/kelch-repeat protein At5g60570-like [Cucurbita moschata] >XP_022964960.1 F... [more]
KAG6583486.14.07e-25593.97F-box/kelch-repeat protein, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1DV434.17e-269100.00F-box/kelch-repeat protein At5g60570-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0LS323.11e-25794.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G084830 PE=4 SV=1[more]
A0A6J1HPP51.25e-25594.25F-box/kelch-repeat protein At5g60570-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5D3BCP02.21e-25593.97F-box/kelch-repeat protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1I4G42.53e-25593.97F-box/kelch-repeat protein At5g60570-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G60570.11.2e-15772.41Galactose oxidase/kelch repeat superfamily protein [more]
AT1G26930.11.9e-9949.03Galactose oxidase/kelch repeat superfamily protein [more]
AT2G02870.12.6e-9649.44Galactose oxidase/kelch repeat superfamily protein [more]
AT2G02870.22.6e-9649.44Galactose oxidase/kelch repeat superfamily protein [more]
AT2G02870.32.6e-9649.44Galactose oxidase/kelch repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 194..246
e-value: 11.0
score: 2.7
coord: 146..193
e-value: 1.0E-5
score: 35.0
coord: 102..145
e-value: 11.0
score: 2.5
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 182..227
e-value: 1.7E-7
score: 30.7
coord: 134..179
e-value: 1.1E-6
score: 28.1
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 53..317
e-value: 6.6E-37
score: 129.0
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 64..304
NoneNo IPR availablePANTHERPTHR46122:SF1GALACTOSE OXIDASE/KELCH REPEAT PROTEINcoord: 5..348
NoneNo IPR availablePANTHERPTHR46122GALACTOSE OXIDASE/KELCH REPEAT PROTEIN-RELATEDcoord: 5..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0280.1MC03g0280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding