MC03g0261 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0261
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMitogen-activated protein kinase
LocationMC03: 7742664 .. 7746141 (+)
RNA-Seq ExpressionMC03g0261
SyntenyMC03g0261
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAAAATAATAAAAAAGTGAGGAAAGAATATTCAAATTTCAAAGTTTCAAAATACAAAGCGCAAAGTGAAAATGGCAGATCAGATGGCGACGCTTTTTCTTCTCCCTCCCCAATACGAAACCCTCTTTTTAACGCTCTCTTAGCTTTCGTTTCACAATTGAAAACTACAGCAACAACAATAAGAAGAAGATGGAGAACGAATCCTCGGCTATGGAGGAGATCAGAGGCCTCCCCACGCACGGAGGAACCTACTTGCTCTACAATGTTCTCGGCAACTTCTTCGAGCTCTCCGCCAAGTACGCCTCTTCCATTCAACCTGTTGGTCGTGGCGCTTACGGCATTGTTTGGTAATTACCACTTCTTCTTTCTTGATTACCACCTCTGCTCTGTTCTTAATTTTCCATTTCTCACTCTGATTTGGGTTCTCAATTCATTCTTGTACTGAGTATGCTGTTTTGTTTTGTCATTTGATTTTCTGTAATACACCCTAATTTCTTTTCTTCTTTCTTTGTTCTTCTTTTTCTCAGTAGGGTGGGTTCATTTCTAGTGCTTCCATTTTCTTTGTTTCAATTTGTTTGTCAAGATTTTGATCATTTGATGTCTCAGTTGTACGACGAATTCGGAGACGAAAGAAGAGGTTGCGATAAAGAAGATAGGGAACGCATTTGACAACAGGATTGATGCCAAGAGGACCCTTCGCGAGATTAAACTCCTGTGCCATATGGACCATGACAATGTATTTATCCGAACTGATGCTCAATTACAATCTTCAATTTGCTGTTATATAACCAAAAACTCTGAAGCTATTTTTGTTATGATTATTTGTTAGCAATTATTGCTTATTCAGGTTGAATTTTAGTTTAATTAATGTGGCTAAACGTGTGGATTGATTATTAATTTTAAAGGGGAGGGAATTTCTTACTTTACTCCATGATAAATTATTATTGAATTTGAAAGCTTGGACTTTTCAGTTACGTGTTTTGAATCTTGTTTCTATATCAGCTGTGCTTTTGTTTTATCATCAACAGATTTCCTTAGTGTTTGATGAAGGGATGGAAAATTTATTGCTGCTATAATTACTTGGAGAGTTTGCCTTGGGGACTTATTTCGTTCTGCCATTGAAGCATAATAATCTCTTTTTTCCCCTTTCCAGATTATAAAAATCAGGGATATAATTCCTCCACCAGAGAAGGAAAAATTCAGTGATGTGTATATTGTGTATGAATTAATGGACACTGACCTGCATCAGATTATTCGCTCTAGCCAGGCTCTGACGGACGATCACTGTCAGGTAAGGGATAACATGAGAGTTACTTTTGATTTGTTGAGGTTTCCTAGTTGATATGCCTGAAACCTACACCTTAGTTTTCTAACAATGTAACTTGCAGTGATTGAGTTTGTTTTGTGGATAATTCTTTTGGGAAGTTGGATCGTGAAACCTAACATCTTGAATTGCATGAATAGAAGACTCTTTTCAAATTGATAGATGGCAATTCCATTCTTTCTTACAGGATCTAACGTTTCTACAAGAAAGAAGCATTTTTCTTATACATATAATTAGAATATGCCTAAAAAAGTTACGCATCCTGTCTATGAAAGATGAGCGATGCCCTAGAGAAGAGAAGAATGATGTTTAACTGGTTCAACTTCCTTGTCATGAAATATTATGCATTGCTAAACAAAATTTTCTCCGGGTGTCTTGTATGATCTGTACTTATAATGTGCTATATACTGAAGGTTTACTTGGAAAATGTTACGTTCCAGTGAGATTTGTTATCAAAAGTCTGTTTCAATATTTAATTCTAGGCTTTATGTCGATAATAATTTTGTTGGAGCATCAACAAACTATCTGAAAGGTTGGTTAGAAATCTTATTCTCCCTGTTAAAGCTGTATCTGTTTATTGTTGGGACAGTACTTCTTGTACCAATTACTGCGAGGTTTGAAGTATCTACACTCTGCTAATGTCTTGCACCGAGATCTAAAACCAAGCAACCTTCTTCTCAATGCAAATTGCGACCTCAAGATTTGTGACTTTGGGTTAGCAAGAACGACCTCGGAGACAGATTTCATGACAGAATATGTTGTAACCCGATGGTATCGAGCCCCTGAACTGCTGCTCAACACTTCTGAATACACTGCAGCAATAGATATTTGGTCTGTTGGATGTATTCTCATGGAAATTCTAAGGAGGGAGCCACTGTTTCCTGGTAAAGACTATGTGCAGCAACTCGGGCTTATAACCGAGGTACTTAATCATTGCCTATCAAATTTCTGCCTACTGTTTAAATTATTTGTGTAGCACTGAGGTGATCTTGTTGTTTCTATAATGTGCTGTTTCATAAATTTCCTGAATTATTATCCGCATTGGTTGCAACTTTAGCTTTTTAATGCTTGTGAATGGAGTTGTTTCTTGATCATTACTACCCCAAACCAGTTGCTAGGTTCACCAGATGATTCAGATCTTGGGTTTCTAAGAAGTGATAATGCAAGGAAGTATGTTAAGCAACTCCCTCATTTCCCGAAGCAACCGCTGACCGAAAAGTTCCCAGATCTTCCTCCACTGGCCGTTGATCTTGCAGAGAGGATGCTAATCTTTGATCCAAGCAAGAGAATAACTGGTAATGCAATGCACCCACCTTTGCATCTAACATAACTTAAACTACCTCTTTTTCTCTTCTCTCCTATATTTTCCTGATATGAGATGTATTTCCTGTCTAAAAGTCGAGGAGGCGATGAGTCACCCATATGTAGTAAGTCTTCATGAAATCAATGAAGAACCAACCAGCCCTGCCCCTTTCAACTTCGATTTTGAACAGGCATCCTTGGATGAAGAAGATATAAAAGAGCTTATATGGAGGGAGTCTCTCAAATTTAATCCAAATTACAAATAGTTTCAAACATTTCGCTCATCTTCAGTTTATTGGGTCTTGATTGAAGAGTTGATTTCGTTTCGAATACTAAACGAGTCCAATAGTGTGAATTGATGTTGTATTAGCGGTTGGCAAGTTGGAACCGAATTTGGGAACAAATAGTTCTTGTATATAATATAATCATGAAGAAGGTTCATTTATCAGGGATTGGATTCACCATCTTGTGGATGATGGCTGGTGTGACATAAGAAAGGGCTGCATCGTCCTCGGCCTCGCATCGAGTTTCTTTGCCTATGTTTTTTAGTACTCTAGGAAGTGAAAATTGTGACTCCTACAGGAAAAAGAAAAAGACAAGAAGATAAAATGTTATGGTTTGCTTCTCTTTTCTGGTATTATTGCTATTGTTTAATACCACAACATACAGTTTTGTTTTTTGGGGATTGTGAGTTCATGTTGACAATGGAATTACTGTTATCAACATTCAAAGCTGATCTTGTTACTGTCACTGTAGAGGCAGGGACTTCAATTAGAATAACTTTAAGGAATCTCATTTTCTTAAATTTTTCCTTTTTAGTAATTAAATTCTAATATATGTGCTTTCACTG

mRNA sequence

CACAAAAATAATAAAAAAGTGAGGAAAGAATATTCAAATTTCAAAGTTTCAAAATACAAAGCGCAAAGTGAAAATGGCAGATCAGATGGCGACGCTTTTTCTTCTCCCTCCCCAATACGAAACCCTCTTTTTAACGCTCTCTTAGCTTTCGTTTCACAATTGAAAACTACAGCAACAACAATAAGAAGAAGATGGAGAACGAATCCTCGGCTATGGAGGAGATCAGAGGCCTCCCCACGCACGGAGGAACCTACTTGCTCTACAATGTTCTCGGCAACTTCTTCGAGCTCTCCGCCAAGTACGCCTCTTCCATTCAACCTGTTGGTCGTGGCGCTTACGGCATTGTTTGTTGTACGACGAATTCGGAGACGAAAGAAGAGGTTGCGATAAAGAAGATAGGGAACGCATTTGACAACAGGATTGATGCCAAGAGGACCCTTCGCGAGATTAAACTCCTGTGCCATATGGACCATGACAATATTATAAAAATCAGGGATATAATTCCTCCACCAGAGAAGGAAAAATTCAGTGATGTGTATATTGTGTATGAATTAATGGACACTGACCTGCATCAGATTATTCGCTCTAGCCAGGCTCTGACGGACGATCACTGTCAGTACTTCTTGTACCAATTACTGCGAGGTTTGAAGTATCTACACTCTGCTAATGTCTTGCACCGAGATCTAAAACCAAGCAACCTTCTTCTCAATGCAAATTGCGACCTCAAGATTTGTGACTTTGGGTTAGCAAGAACGACCTCGGAGACAGATTTCATGACAGAATATGTTGTAACCCGATGGTATCGAGCCCCTGAACTGCTGCTCAACACTTCTGAATACACTGCAGCAATAGATATTTGGTCTGTTGGATGTATTCTCATGGAAATTCTAAGGAGGGAGCCACTGTTTCCTGGTAAAGACTATGTGCAGCAACTCGGGCTTATAACCGAGTTGCTAGGTTCACCAGATGATTCAGATCTTGGGTTTCTAAGAAGTGATAATGCAAGGAAGTATGTTAAGCAACTCCCTCATTTCCCGAAGCAACCGCTGACCGAAAAGTTCCCAGATCTTCCTCCACTGGCCGTTGATCTTGCAGAGAGGATGCTAATCTTTGATCCAAGCAAGAGAATAACTGTCGAGGAGGCGATGAGTCACCCATATGTAGTAAGTCTTCATGAAATCAATGAAGAACCAACCAGCCCTGCCCCTTTCAACTTCGATTTTGAACAGGCATCCTTGGATGAAGAAGATATAAAAGAGCTTATATGGAGGGAGTCTCTCAAATTTAATCCAAATTACAAATAGTTTCAAACATTTCGCTCATCTTCAGTTTATTGGGTCTTGATTGAAGAGTTGATTTCGTTTCGAATACTAAACGAGTCCAATAGTGTGAATTGATGTTGTATTAGCGGTTGGCAAGTTGGAACCGAATTTGGGAACAAATAGTTCTTGTATATAATATAATCATGAAGAAGGTTCATTTATCAGGGATTGGATTCACCATCTTGTGGATGATGGCTGGTGTGACATAAGAAAGGGCTGCATCGTCCTCGGCCTCGCATCGAGTTTCTTTGCCTATGTTTTTTAGTACTCTAGGAAGTGAAAATTGTGACTCCTACAGGAAAAAGAAAAAGACAAGAAGATAAAATGTTATGGTTTGCTTCTCTTTTCTGGTATTATTGCTATTGTTTAATACCACAACATACAGTTTTGTTTTTTGGGGATTGTGAGTTCATGTTGACAATGGAATTACTGTTATCAACATTCAAAGCTGATCTTGTTACTGTCACTGTAGAGGCAGGGACTTCAATTAGAATAACTTTAAGGAATCTCATTTTCTTAAATTTTTCCTTTTTAGTAATTAAATTCTAATATATGTGCTTTCACTG

Coding sequence (CDS)

ATGGAGAACGAATCCTCGGCTATGGAGGAGATCAGAGGCCTCCCCACGCACGGAGGAACCTACTTGCTCTACAATGTTCTCGGCAACTTCTTCGAGCTCTCCGCCAAGTACGCCTCTTCCATTCAACCTGTTGGTCGTGGCGCTTACGGCATTGTTTGTTGTACGACGAATTCGGAGACGAAAGAAGAGGTTGCGATAAAGAAGATAGGGAACGCATTTGACAACAGGATTGATGCCAAGAGGACCCTTCGCGAGATTAAACTCCTGTGCCATATGGACCATGACAATATTATAAAAATCAGGGATATAATTCCTCCACCAGAGAAGGAAAAATTCAGTGATGTGTATATTGTGTATGAATTAATGGACACTGACCTGCATCAGATTATTCGCTCTAGCCAGGCTCTGACGGACGATCACTGTCAGTACTTCTTGTACCAATTACTGCGAGGTTTGAAGTATCTACACTCTGCTAATGTCTTGCACCGAGATCTAAAACCAAGCAACCTTCTTCTCAATGCAAATTGCGACCTCAAGATTTGTGACTTTGGGTTAGCAAGAACGACCTCGGAGACAGATTTCATGACAGAATATGTTGTAACCCGATGGTATCGAGCCCCTGAACTGCTGCTCAACACTTCTGAATACACTGCAGCAATAGATATTTGGTCTGTTGGATGTATTCTCATGGAAATTCTAAGGAGGGAGCCACTGTTTCCTGGTAAAGACTATGTGCAGCAACTCGGGCTTATAACCGAGTTGCTAGGTTCACCAGATGATTCAGATCTTGGGTTTCTAAGAAGTGATAATGCAAGGAAGTATGTTAAGCAACTCCCTCATTTCCCGAAGCAACCGCTGACCGAAAAGTTCCCAGATCTTCCTCCACTGGCCGTTGATCTTGCAGAGAGGATGCTAATCTTTGATCCAAGCAAGAGAATAACTGTCGAGGAGGCGATGAGTCACCCATATGTAGTAAGTCTTCATGAAATCAATGAAGAACCAACCAGCCCTGCCCCTTTCAACTTCGATTTTGAACAGGCATCCTTGGATGAAGAAGATATAAAAGAGCTTATATGGAGGGAGTCTCTCAAATTTAATCCAAATTACAAATAG

Protein sequence

MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNPNYK
Homology
BLAST of MC03g0261 vs. ExPASy Swiss-Prot
Match: Q40531 (Mitogen-activated protein kinase homolog NTF6 OS=Nicotiana tabacum OX=4097 GN=NTF6 PE=2 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 5.5e-182
Identity = 299/368 (81.25%), Postives = 340/368 (92.39%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MENE++   EI+G+PTH G Y+ YNVLGNFFE+++KY   IQPVGRGAYG+VCC TNSET
Sbjct: 1   MENETNEKLEIKGIPTHEGKYVEYNVLGNFFEVTSKYIPPIQPVGRGAYGMVCCATNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAF+NRIDAKRTLREIKLL HMDH+NIIKI+DI+ PP++E+F+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFENRIDAKRTLREIKLLSHMDHENIIKIKDIVRPPDREEFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSE DFMTEYVVTRWYRAPELLLN +EYTAAIDIWSVGCILME+++REPLFP
Sbjct: 181 CDFGLARTTSEADFMTEYVVTRWYRAPELLLNCTEYTAAIDIWSVGCILMELIKREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           G+DY QQLGLI  LLGSP+DSDLGFLRSDNARKYVK LP  P+ P ++KFPD+ PLA+DL
Sbjct: 241 GRDYAQQLGLIIALLGSPEDSDLGFLRSDNARKYVKHLPRVPRHPFSQKFPDVSPLALDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDP+KRITVE+A++HP+++SLHEINEEP   +PFNFDFEQASL E+DIKELIW 
Sbjct: 301 AERMLVFDPAKRITVEDALNHPFLISLHEINEEPVCDSPFNFDFEQASLSEDDIKELIWN 360

Query: 361 ESLKFNPN 369
           E+LKF+PN
Sbjct: 361 EALKFDPN 368

BLAST of MC03g0261 vs. ExPASy Swiss-Prot
Match: Q9LQQ9 (Mitogen-activated protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=MPK13 PE=1 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 1.5e-171
Identity = 285/353 (80.74%), Postives = 318/353 (90.08%), Query Frame = 0

Query: 13  GLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGNA 72
           G+ T+ G Y++YNVLGN FELS+KY   I+P+GRGAYGIVCC TNSET EEVAIKKI NA
Sbjct: 8   GILTYDGRYVMYNVLGNIFELSSKYIPPIEPIGRGAYGIVCCATNSETNEEVAIKKIANA 67

Query: 73  FDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQIIRS 132
           FDNR+DAKRTLREIKLL HMDHDN+IKI+DII  PEKE+F DVYIVYELMDTDLHQIIRS
Sbjct: 68  FDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDVYIVYELMDTDLHQIIRS 127

Query: 133 SQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSET 192
           +Q LTDDHCQYFLYQ+LRGLKY+HSANVLHRDLKPSNL+LN NCDLKICDFGLART++ET
Sbjct: 128 TQTLTDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLVLNTNCDLKICDFGLARTSNET 187

Query: 193 DFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLIT 252
           + MTEYVVTRWYRAPELLLN+SEYT AIDIWSVGCI MEILRRE LFPGKDYVQQL LIT
Sbjct: 188 EIMTEYVVTRWYRAPELLLNSSEYTGAIDIWSVGCIFMEILRRETLFPGKDYVQQLKLIT 247

Query: 253 ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPSKR 312
           ELLGSPDDSDL FLRSDNARKYVKQLPH  KQ   EKFP++ P+A+DLAE+ML+FDPSKR
Sbjct: 248 ELLGSPDDSDLDFLRSDNARKYVKQLPHVQKQSFREKFPNISPMALDLAEKMLVFDPSKR 307

Query: 313 ITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKF 366
           ITV+EA+  PY+ SLHEINEEPT P PF+FDFE+ +LDE+DIKEL+WRESL F
Sbjct: 308 ITVDEALKQPYLASLHEINEEPTCPTPFSFDFEETALDEQDIKELVWRESLHF 360

BLAST of MC03g0261 vs. ExPASy Swiss-Prot
Match: A9S9Q8 (Mitogen-activated protein kinase 4b OS=Physcomitrium patens OX=3218 GN=MPK4b PE=1 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-164
Identity = 274/357 (76.75%), Postives = 310/357 (86.83%), Query Frame = 0

Query: 11  IRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIG 70
           I+G+PTH G Y  YN+ GN FE+S KY   I+P+GRGAYGIVC   NSET EEVAIKKIG
Sbjct: 16  IQGVPTHNGEYTQYNIFGNLFEVSRKYVPPIRPIGRGAYGIVCSAVNSETGEEVAIKKIG 75

Query: 71  NAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQII 130
           NAFDNRIDAKRTLREIKLL HMDH+NI+ IRDII PP +E F+DVYIVYELMDTDLHQII
Sbjct: 76  NAFDNRIDAKRTLREIKLLRHMDHENIVAIRDIIRPPTRENFNDVYIVYELMDTDLHQII 135

Query: 131 RSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTS 190
           RS+Q LT+DHCQYFLYQLLRGLKY+HSA VLHRDLKPSNLLLNANCDLKICDFGLARTTS
Sbjct: 136 RSNQPLTEDHCQYFLYQLLRGLKYIHSAKVLHRDLKPSNLLLNANCDLKICDFGLARTTS 195

Query: 191 ETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGL 250
           ETDFMTEYVVTRWYRAPELLLN SEYTAAID+WSVGCI ME+L REPLFPG+DYVQQL L
Sbjct: 196 ETDFMTEYVVTRWYRAPELLLNCSEYTAAIDVWSVGCIFMELLNREPLFPGRDYVQQLRL 255

Query: 251 ITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPS 310
           ITEL+GSP+D DLGFLRSDNAR+Y++QLP F +QPL  KFP++ P A+DL E ML FDP+
Sbjct: 256 ITELIGSPEDHDLGFLRSDNARRYIRQLPRFARQPLDRKFPNMGPAAIDLVEHMLRFDPA 315

Query: 311 KRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNP 368
           +RITVEEA++HPY+ +LH+IN+EP   +PF FDFEQ S  EE IKELI  E++ FNP
Sbjct: 316 RRITVEEALAHPYLATLHDINDEPICHSPFEFDFEQPSFTEEHIKELIMMEAIAFNP 372

BLAST of MC03g0261 vs. ExPASy Swiss-Prot
Match: Q336X9 (Mitogen-activated protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MPK6 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.0e-164
Identity = 276/359 (76.88%), Postives = 315/359 (87.74%), Query Frame = 0

Query: 10  EIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKI 69
           +I+G+ THGG Y+LYNV GNFFE+S+KYA  I+P+GRGAYGIVC   NSE  EEVAIKKI
Sbjct: 15  QIKGMGTHGGRYVLYNVYGNFFEVSSKYAPPIRPIGRGAYGIVCAAVNSENGEEVAIKKI 74

Query: 70  GNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQI 129
           GNAFDN IDAKRTLREIKLL HMDH+NII I+DII PP ++ F+DVYIV ELMDTDLHQI
Sbjct: 75  GNAFDNHIDAKRTLREIKLLRHMDHENIIAIKDIIRPPRRDNFNDVYIVSELMDTDLHQI 134

Query: 130 IRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTT 189
           IRS+Q LTDDHCQYFLYQLLRGLKY+HSANVLHRDLKPSNL LNANCDLKI DFGLARTT
Sbjct: 135 IRSNQPLTDDHCQYFLYQLLRGLKYVHSANVLHRDLKPSNLFLNANCDLKIADFGLARTT 194

Query: 190 SETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLG 249
           +ETD MTEYVVTRWYRAPELLLN S+YTAAID+WSVGCIL EI+ R+PLFPG+DY+QQL 
Sbjct: 195 TETDLMTEYVVTRWYRAPELLLNCSQYTAAIDVWSVGCILGEIVTRQPLFPGRDYIQQLK 254

Query: 250 LITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDP 309
           LITEL+GSPDDS LGFLRSDNAR+Y+KQLP +P+Q    +F ++   AVDL E+ML+FDP
Sbjct: 255 LITELIGSPDDSSLGFLRSDNARRYMKQLPQYPRQDFRLRFRNMSAGAVDLLEKMLVFDP 314

Query: 310 SKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNPN 369
           S+RITV+EA+ HPY+ SLH+INEEPT PAPF+FDFEQ S  EE IKELIWRESL FNP+
Sbjct: 315 SRRITVDEALHHPYLASLHDINEEPTCPAPFSFDFEQPSFTEEHIKELIWRESLAFNPD 373

BLAST of MC03g0261 vs. ExPASy Swiss-Prot
Match: Q40353 (Mitogen-activated protein kinase homolog MMK2 OS=Medicago sativa OX=3879 GN=MMK2 PE=2 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 4.4e-163
Identity = 272/363 (74.93%), Postives = 315/363 (86.78%), Query Frame = 0

Query: 6   SAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVA 65
           SA   IRG+PTHGG YL YN+ GN FE+S KY   I+ VGRGAYGIVC   N+ET+EEVA
Sbjct: 5   SAENNIRGVPTHGGRYLQYNIYGNLFEVSRKYVPPIRSVGRGAYGIVCAAVNAETREEVA 64

Query: 66  IKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTD 125
           IKKIGNAFDNRIDAKRTLREIKLL HMDH+N++ I+DII PP+KE F+ VYIV ELMDTD
Sbjct: 65  IKKIGNAFDNRIDAKRTLREIKLLRHMDHENVMSIKDIIRPPQKENFNHVYIVSELMDTD 124

Query: 126 LHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGL 185
           LHQIIRS+Q +TDDHC+YF+YQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLKI DFGL
Sbjct: 125 LHQIIRSNQPMTDDHCRYFVYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGL 184

Query: 186 ARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYV 245
           ARTTSETDFMTEYVVTRWYRAPELLLN S+YTAAIDIWSVGCIL EI+ R+PLFPG+DYV
Sbjct: 185 ARTTSETDFMTEYVVTRWYRAPELLLNCSDYTAAIDIWSVGCILGEIVTRQPLFPGRDYV 244

Query: 246 QQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERML 305
            QL L+TEL+GSPDD+ LGFLRS+NAR+YV+QLP +PKQ  + +FP++ P AVDL E+ML
Sbjct: 245 HQLRLVTELIGSPDDASLGFLRSENARRYVRQLPQYPKQNFSARFPNMSPGAVDLLEKML 304

Query: 306 IFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKF 365
           IFDPSKRI V+EA+ HPY+  LH+INEEP    PF+FDFE+    EEDIKELIW+ES++F
Sbjct: 305 IFDPSKRIKVDEALCHPYMAPLHDINEEPVCARPFSFDFEEPMFTEEDIKELIWKESVRF 364

Query: 366 NPN 369
           NP+
Sbjct: 365 NPD 367

BLAST of MC03g0261 vs. NCBI nr
Match: XP_022156700.1 (mitogen-activated protein kinase homolog NTF6-like [Momordica charantia])

HSP 1 Score: 754 bits (1948), Expect = 3.77e-275
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR
Sbjct: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360

Query: 361 ESLKFNPNYK 370
           ESLKFNPNYK
Sbjct: 361 ESLKFNPNYK 370

BLAST of MC03g0261 vs. NCBI nr
Match: XP_022970376.1 (mitogen-activated protein kinase homolog NTF6 [Cucurbita maxima])

HSP 1 Score: 715 bits (1846), Expect = 1.28e-259
Identity = 348/369 (94.31%), Postives = 361/369 (97.83%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SSAME I+G PT+ G YLLYNVLGNFFE+SAKYA SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSAME-IKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREI+LLCHMDHDNIIKI+DIIPPPEKEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIW+VGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWAVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY+VSLHEINEEPT P+PFNFDFEQASLDEE+IKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEEIKELIWR 360

Query: 361 ESLKFNPNY 369
            SLKFNPNY
Sbjct: 361 ASLKFNPNY 368

BLAST of MC03g0261 vs. NCBI nr
Match: XP_022964848.1 (mitogen-activated protein kinase homolog NTF6 [Cucurbita moschata] >XP_023519830.1 mitogen-activated protein kinase homolog NTF6-like [Cucurbita pepo subsp. pepo] >KAG6583474.1 Mitogen-activated protein kinase-like NTF6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 714 bits (1843), Expect = 3.68e-259
Identity = 347/369 (94.04%), Postives = 361/369 (97.83%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SSAME I+G PT+ G YLLYNVLGNFFE+SAKYA SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSAME-IKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREI+LLCHMDHDNIIKI+DIIPPPEKEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIW+VGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWAVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY++SLHEINEEPT P+PFNFDFEQASLDEE+IKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYILSLHEINEEPTCPSPFNFDFEQASLDEEEIKELIWR 360

Query: 361 ESLKFNPNY 369
            SLKFNPNY
Sbjct: 361 ASLKFNPNY 368

BLAST of MC03g0261 vs. NCBI nr
Match: XP_038893703.1 (mitogen-activated protein kinase homolog NTF6 [Benincasa hispida])

HSP 1 Score: 712 bits (1839), Expect = 1.61e-258
Identity = 346/370 (93.51%), Postives = 361/370 (97.57%), Query Frame = 0

Query: 1   MENESSAME-EIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSE 60
           MEN+SS+   +I+G+PT+   YLLYNVLGNFFE+SAKY+ SIQPVGRGAYGIVCCTTNSE
Sbjct: 1   MENDSSSSAMDIKGIPTYDSKYLLYNVLGNFFEISAKYSPSIQPVGRGAYGIVCCTTNSE 60

Query: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVY 120
           TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKI+DIIPPPEKEKF+DVYIVY
Sbjct: 61  TKEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVY 120

Query: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180
           ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK
Sbjct: 121 ELMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLK 180

Query: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240
           ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF
Sbjct: 181 ICDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLF 240

Query: 241 PGKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300
           PGKDYVQQLGLI ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD
Sbjct: 241 PGKDYVQQLGLIIELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVD 300

Query: 301 LAERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIW 360
           LAERML+FDPSKRITVEEAM+HPY+VSLHEINEEPT P+PFNFDFEQASLDEEDIKELIW
Sbjct: 301 LAERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIW 360

Query: 361 RESLKFNPNY 369
           RESLKFNPN+
Sbjct: 361 RESLKFNPNH 370

BLAST of MC03g0261 vs. NCBI nr
Match: XP_004148790.2 (mitogen-activated protein kinase homolog NTF6 [Cucumis sativus] >KGN64720.1 hypothetical protein Csa_013975 [Cucumis sativus])

HSP 1 Score: 711 bits (1836), Expect = 4.45e-258
Identity = 343/369 (92.95%), Postives = 360/369 (97.56%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SS+  +I+G PT+   YLLYNVLG+FFE+SAKY+ SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKI+DIIPPP+KEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY+VSLHEINEEPT P+PFNFDFEQASLDEEDIKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWR 360

Query: 361 ESLKFNPNY 369
           ES+KFNPN+
Sbjct: 361 ESIKFNPNH 369

BLAST of MC03g0261 vs. ExPASy TrEMBL
Match: A0A6J1DR23 (Mitogen-activated protein kinase OS=Momordica charantia OX=3673 GN=LOC111023545 PE=3 SV=1)

HSP 1 Score: 754 bits (1948), Expect = 1.82e-275
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR
Sbjct: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360

Query: 361 ESLKFNPNYK 370
           ESLKFNPNYK
Sbjct: 361 ESLKFNPNYK 370

BLAST of MC03g0261 vs. ExPASy TrEMBL
Match: A0A6J1I0F3 (Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111469378 PE=3 SV=1)

HSP 1 Score: 715 bits (1846), Expect = 6.21e-260
Identity = 348/369 (94.31%), Postives = 361/369 (97.83%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SSAME I+G PT+ G YLLYNVLGNFFE+SAKYA SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSAME-IKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREI+LLCHMDHDNIIKI+DIIPPPEKEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIW+VGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWAVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY+VSLHEINEEPT P+PFNFDFEQASLDEE+IKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEEIKELIWR 360

Query: 361 ESLKFNPNY 369
            SLKFNPNY
Sbjct: 361 ASLKFNPNY 368

BLAST of MC03g0261 vs. ExPASy TrEMBL
Match: A0A6J1HM42 (Mitogen-activated protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111464829 PE=3 SV=1)

HSP 1 Score: 714 bits (1843), Expect = 1.78e-259
Identity = 347/369 (94.04%), Postives = 361/369 (97.83%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SSAME I+G PT+ G YLLYNVLGNFFE+SAKYA SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSAME-IKGFPTYDGKYLLYNVLGNFFEVSAKYAPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREI+LLCHMDHDNIIKI+DIIPPPEKEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIELLCHMDHDNIIKIKDIIPPPEKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIW+VGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWAVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY++SLHEINEEPT P+PFNFDFEQASLDEE+IKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYILSLHEINEEPTCPSPFNFDFEQASLDEEEIKELIWR 360

Query: 361 ESLKFNPNY 369
            SLKFNPNY
Sbjct: 361 ASLKFNPNY 368

BLAST of MC03g0261 vs. ExPASy TrEMBL
Match: A0A0A0LS13 (Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G077220 PE=3 SV=1)

HSP 1 Score: 711 bits (1836), Expect = 2.16e-258
Identity = 343/369 (92.95%), Postives = 360/369 (97.56%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SS+  +I+G PT+   YLLYNVLG+FFE+SAKY+ SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKI+DIIPPP+KEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY+VSLHEINEEPT P+PFNFDFEQASLDEEDIKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWR 360

Query: 361 ESLKFNPNY 369
           ES+KFNPN+
Sbjct: 361 ESIKFNPNH 369

BLAST of MC03g0261 vs. ExPASy TrEMBL
Match: A0A1S3CNX4 (Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103502937 PE=3 SV=1)

HSP 1 Score: 709 bits (1830), Expect = 1.77e-257
Identity = 342/369 (92.68%), Postives = 359/369 (97.29%), Query Frame = 0

Query: 1   MENESSAMEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSET 60
           MEN+SS+  +I+G PT+   YLLYNVLG+FFE+SAKY+ SIQPVGRGAYGIVCCTTNSET
Sbjct: 1   MENDSSSAMDIKGTPTYDSKYLLYNVLGSFFEVSAKYSPSIQPVGRGAYGIVCCTTNSET 60

Query: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYE 120
           KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKI+DIIPPP+KEKF+DVYIVYE
Sbjct: 61  KEEVAIKKIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIKDIIPPPDKEKFNDVYIVYE 120

Query: 121 LMDTDLHQIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180
           LMDTDLHQIIRSSQ LTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI
Sbjct: 121 LMDTDLHQIIRSSQDLTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKI 180

Query: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240
           CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP
Sbjct: 181 CDFGLARTTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFP 240

Query: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDL 300
           GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPL EKFPDLPPLAVDL
Sbjct: 241 GKDYVQQLGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLIEKFPDLPPLAVDL 300

Query: 301 AERMLIFDPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWR 360
           AERML+FDPSKRITVEEAM+HPY+VSLHEINEEPT P+PFNFDFEQASLDEEDIKELIWR
Sbjct: 301 AERMLLFDPSKRITVEEAMNHPYIVSLHEINEEPTCPSPFNFDFEQASLDEEDIKELIWR 360

Query: 361 ESLKFNPNY 369
           ES+KFNPN+
Sbjct: 361 ESIKFNPNH 369

BLAST of MC03g0261 vs. TAIR 10
Match: AT1G07880.2 (Protein kinase superfamily protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.1e-172
Identity = 285/353 (80.74%), Postives = 318/353 (90.08%), Query Frame = 0

Query: 13  GLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGNA 72
           G+ T+ G Y++YNVLGN FELS+KY   I+P+GRGAYGIVCC TNSET EEVAIKKI NA
Sbjct: 8   GILTYDGRYVMYNVLGNIFELSSKYIPPIEPIGRGAYGIVCCATNSETNEEVAIKKIANA 67

Query: 73  FDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQIIRS 132
           FDNR+DAKRTLREIKLL HMDHDN+IKI+DII  PEKE+F DVYIVYELMDTDLHQIIRS
Sbjct: 68  FDNRVDAKRTLREIKLLSHMDHDNVIKIKDIIELPEKERFEDVYIVYELMDTDLHQIIRS 127

Query: 133 SQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSET 192
           +Q LTDDHCQYFLYQ+LRGLKY+HSANVLHRDLKPSNL+LN NCDLKICDFGLART++ET
Sbjct: 128 TQTLTDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLVLNTNCDLKICDFGLARTSNET 187

Query: 193 DFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLIT 252
           + MTEYVVTRWYRAPELLLN+SEYT AIDIWSVGCI MEILRRE LFPGKDYVQQL LIT
Sbjct: 188 EIMTEYVVTRWYRAPELLLNSSEYTGAIDIWSVGCIFMEILRRETLFPGKDYVQQLKLIT 247

Query: 253 ELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPSKR 312
           ELLGSPDDSDL FLRSDNARKYVKQLPH  KQ   EKFP++ P+A+DLAE+ML+FDPSKR
Sbjct: 248 ELLGSPDDSDLDFLRSDNARKYVKQLPHVQKQSFREKFPNISPMALDLAEKMLVFDPSKR 307

Query: 313 ITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKF 366
           ITV+EA+  PY+ SLHEINEEPT P PF+FDFE+ +LDE+DIKEL+WRESL F
Sbjct: 308 ITVDEALKQPYLASLHEINEEPTCPTPFSFDFEETALDEQDIKELVWRESLHF 360

BLAST of MC03g0261 vs. TAIR 10
Match: AT4G01370.1 (MAP kinase 4 )

HSP 1 Score: 572.0 bits (1473), Expect = 3.4e-163
Identity = 271/356 (76.12%), Postives = 312/356 (87.64%), Query Frame = 0

Query: 12  RGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGN 71
           +G+ THGG+Y+ YNV GN FE+S KY   ++P+GRGAYGIVC  TNSET EEVAIKKIGN
Sbjct: 17  KGVATHGGSYVQYNVYGNLFEVSRKYVPPLRPIGRGAYGIVCAATNSETGEEVAIKKIGN 76

Query: 72  AFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQIIR 131
           AFDN IDAKRTLREIKLL HMDH+N+I ++DII PP++E F+DVYIVYELMDTDLHQIIR
Sbjct: 77  AFDNIIDAKRTLREIKLLKHMDHENVIAVKDIIKPPQRENFNDVYIVYELMDTDLHQIIR 136

Query: 132 SSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSE 191
           S+Q LTDDHC++FLYQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLK+ DFGLART SE
Sbjct: 137 SNQPLTDDHCRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKLGDFGLARTKSE 196

Query: 192 TDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLI 251
           TDFMTEYVVTRWYRAPELLLN SEYTAAIDIWSVGCIL E + REPLFPGKDYV QL LI
Sbjct: 197 TDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGETMTREPLFPGKDYVHQLRLI 256

Query: 252 TELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPSK 311
           TEL+GSPDDS LGFLRSDNAR+YV+QLP +P+Q    +FP++   AVDL E+ML+FDPS+
Sbjct: 257 TELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSAGAVDLLEKMLVFDPSR 316

Query: 312 RITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNP 368
           RITV+EA+ HPY+  LH+INEEP    PFNFDFEQ +L EE+IKELI+RE++KFNP
Sbjct: 317 RITVDEALCHPYLAPLHDINEEPVCVRPFNFDFEQPTLTEENIKELIYRETVKFNP 372

BLAST of MC03g0261 vs. TAIR 10
Match: AT1G01560.2 (MAP kinase 11 )

HSP 1 Score: 548.9 bits (1413), Expect = 3.1e-156
Identity = 266/356 (74.72%), Postives = 304/356 (85.39%), Query Frame = 0

Query: 12  RGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIGN 71
           RG+  +GG Y+ YNV GN FE+S KY   ++P+GRGA GIVC   NSET EEVAIKKIGN
Sbjct: 14  RGVSINGGRYVQYNVYGNLFEVSKKYVPPLRPIGRGASGIVCAAWNSETGEEVAIKKIGN 73

Query: 72  AFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQIIR 131
           AF N IDAKRTLREIKLL HMDHDN+I I DII PP+ + F+DV+IVYELMDTDLH IIR
Sbjct: 74  AFGNIIDAKRTLREIKLLKHMDHDNVIAIIDIIRPPQPDNFNDVHIVYELMDTDLHHIIR 133

Query: 132 SSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTSE 191
           S+Q LTDDH ++FLYQLLRGLKY+HSANVLHRDLKPSNLLLNANCDLKI DFGLART SE
Sbjct: 134 SNQPLTDDHSRFFLYQLLRGLKYVHSANVLHRDLKPSNLLLNANCDLKIGDFGLARTKSE 193

Query: 192 TDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGLI 251
           TDFMTEYVVTRWYRAPELLLN SEYTAAIDIWSVGCIL EI+ REPLFPG+DYVQQL LI
Sbjct: 194 TDFMTEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEIMTREPLFPGRDYVQQLRLI 253

Query: 252 TELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPSK 311
           TEL+GSPDDS LGFLRSDNAR+YV+QLP +P+Q    +FP++   AVDL ++ML+FDP++
Sbjct: 254 TELIGSPDDSSLGFLRSDNARRYVRQLPQYPRQNFAARFPNMSVNAVDLLQKMLVFDPNR 313

Query: 312 RITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNP 368
           RITV+EA+ HPY+  LHE NEEP    PF+FDFEQ SL EE+IKELI+RES+KFNP
Sbjct: 314 RITVDEALCHPYLAPLHEYNEEPVCVRPFHFDFEQPSLTEENIKELIYRESVKFNP 369

BLAST of MC03g0261 vs. TAIR 10
Match: AT2G43790.1 (MAP kinase 6 )

HSP 1 Score: 538.9 bits (1387), Expect = 3.2e-153
Identity = 249/363 (68.60%), Postives = 309/363 (85.12%), Query Frame = 0

Query: 8   MEEIRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIK 67
           +E I    +HGG ++ YN+ GN FE++AKY   I P+G+GAYGIVC   NSET E VAIK
Sbjct: 33  IENIPATLSHGGRFIQYNIFGNIFEVTAKYKPPIMPIGKGAYGIVCSAMNSETNESVAIK 92

Query: 68  KIGNAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLH 127
           KI NAFDN+IDAKRTLREIKLL HMDH+NI+ IRDIIPPP +  F+DVYI YELMDTDLH
Sbjct: 93  KIANAFDNKIDAKRTLREIKLLRHMDHENIVAIRDIIPPPLRNAFNDVYIAYELMDTDLH 152

Query: 128 QIIRSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLAR 187
           QIIRS+QAL+++HCQYFLYQ+LRGLKY+HSANVLHRDLKPSNLLLNANCDLKICDFGLAR
Sbjct: 153 QIIRSNQALSEEHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNANCDLKICDFGLAR 212

Query: 188 TTSETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQ 247
            TSE+DFMTEYVVTRWYRAPELLLN+S+YTAAID+WSVGCI ME++ R+PLFPG+D+V Q
Sbjct: 213 VTSESDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQ 272

Query: 248 LGLITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIF 307
           L L+ EL+G+P + +L FL ++NA++Y++QLP +P+Q +T+KFP + PLA+DL E+ML F
Sbjct: 273 LRLLMELIGTPSEEELEFL-NENAKRYIRQLPPYPRQSITDKFPTVHPLAIDLIEKMLTF 332

Query: 308 DPSKRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNP 367
           DP +RITV +A++HPY+ SLH+I++EP    PFNFDFE  +L EE +KELI+RE+L FNP
Sbjct: 333 DPRRRITVLDALAHPYLNSLHDISDEPECTIPFNFDFENHALSEEQMKELIYREALAFNP 392

Query: 368 NYK 371
            Y+
Sbjct: 393 EYQ 394

BLAST of MC03g0261 vs. TAIR 10
Match: AT4G11330.1 (MAP kinase 5 )

HSP 1 Score: 538.9 bits (1387), Expect = 3.2e-153
Identity = 252/357 (70.59%), Postives = 301/357 (84.31%), Query Frame = 0

Query: 11  IRGLPTHGGTYLLYNVLGNFFELSAKYASSIQPVGRGAYGIVCCTTNSETKEEVAIKKIG 70
           I+G+  HGG Y  YNV GN FE+S KY   I+P+GRGAYG VC   +SET EE+AIKKIG
Sbjct: 16  IKGVLVHGGRYFQYNVYGNLFEVSNKYVPPIRPIGRGAYGFVCAAVDSETHEEIAIKKIG 75

Query: 71  NAFDNRIDAKRTLREIKLLCHMDHDNIIKIRDIIPPPEKEKFSDVYIVYELMDTDLHQII 130
            AFDN++DAKRTLREIKLL H++H+N++ I+DII PP+KE F DVYIV+ELMDTDLHQII
Sbjct: 76  KAFDNKVDAKRTLREIKLLRHLEHENVVVIKDIIRPPKKEDFVDVYIVFELMDTDLHQII 135

Query: 131 RSSQALTDDHCQYFLYQLLRGLKYLHSANVLHRDLKPSNLLLNANCDLKICDFGLARTTS 190
           RS+Q+L DDHCQYFLYQ+LRGLKY+HSANVLHRDLKPSNLLLN+NCDLKI DFGLARTTS
Sbjct: 136 RSNQSLNDDHCQYFLYQILRGLKYIHSANVLHRDLKPSNLLLNSNCDLKITDFGLARTTS 195

Query: 191 ETDFMTEYVVTRWYRAPELLLNTSEYTAAIDIWSVGCILMEILRREPLFPGKDYVQQLGL 250
           ET++MTEYVVTRWYRAPELLLN+SEYT+AID+WSVGCI  EI+ REPLFPGKDYV QL L
Sbjct: 196 ETEYMTEYVVTRWYRAPELLLNSSEYTSAIDVWSVGCIFAEIMTREPLFPGKDYVHQLKL 255

Query: 251 ITELLGSPDDSDLGFLRSDNARKYVKQLPHFPKQPLTEKFPDLPPLAVDLAERMLIFDPS 310
           ITEL+GSPD + L FLRS NARKYVK+LP FP+Q  + +FP +   A+DL E+ML+FDP 
Sbjct: 256 ITELIGSPDGASLEFLRSANARKYVKELPKFPRQNFSARFPSMNSTAIDLLEKMLVFDPV 315

Query: 311 KRITVEEAMSHPYVVSLHEINEEPTSPAPFNFDFEQASLDEEDIKELIWRESLKFNP 368
           KRITVEEA+ +PY+ +LH++N+EP     F+F FE  S  EE+IKEL+W ES+KFNP
Sbjct: 316 KRITVEEALCYPYLSALHDLNDEPVCSNHFSFHFEDPSSTEEEIKELVWLESVKFNP 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q405315.5e-18281.25Mitogen-activated protein kinase homolog NTF6 OS=Nicotiana tabacum OX=4097 GN=NT... [more]
Q9LQQ91.5e-17180.74Mitogen-activated protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=MPK13 PE=... [more]
A9S9Q81.0e-16476.75Mitogen-activated protein kinase 4b OS=Physcomitrium patens OX=3218 GN=MPK4b PE=... [more]
Q336X91.0e-16476.88Mitogen-activated protein kinase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q403534.4e-16374.93Mitogen-activated protein kinase homolog MMK2 OS=Medicago sativa OX=3879 GN=MMK2... [more]
Match NameE-valueIdentityDescription
XP_022156700.13.77e-275100.00mitogen-activated protein kinase homolog NTF6-like [Momordica charantia][more]
XP_022970376.11.28e-25994.31mitogen-activated protein kinase homolog NTF6 [Cucurbita maxima][more]
XP_022964848.13.68e-25994.04mitogen-activated protein kinase homolog NTF6 [Cucurbita moschata] >XP_023519830... [more]
XP_038893703.11.61e-25893.51mitogen-activated protein kinase homolog NTF6 [Benincasa hispida][more]
XP_004148790.24.45e-25892.95mitogen-activated protein kinase homolog NTF6 [Cucumis sativus] >KGN64720.1 hypo... [more]
Match NameE-valueIdentityDescription
A0A6J1DR231.82e-275100.00Mitogen-activated protein kinase OS=Momordica charantia OX=3673 GN=LOC111023545 ... [more]
A0A6J1I0F36.21e-26094.31Mitogen-activated protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111469378 PE=... [more]
A0A6J1HM421.78e-25994.04Mitogen-activated protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111464829 P... [more]
A0A0A0LS132.16e-25892.95Mitogen-activated protein kinase OS=Cucumis sativus OX=3659 GN=Csa_1G077220 PE=3... [more]
A0A1S3CNX41.77e-25792.68Mitogen-activated protein kinase OS=Cucumis melo OX=3656 GN=LOC103502937 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT1G07880.21.1e-17280.74Protein kinase superfamily protein [more]
AT4G01370.13.4e-16376.12MAP kinase 4 [more]
AT1G01560.23.1e-15674.72MAP kinase 11 [more]
AT2G43790.13.2e-15368.60MAP kinase 6 [more]
AT4G11330.13.2e-15370.59MAP kinase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 38..324
e-value: 1.9E-92
score: 323.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 41..324
e-value: 1.4E-69
score: 234.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 38..324
score: 44.625999
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 122..351
e-value: 1.6E-156
score: 522.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 21..361
e-value: 1.6E-156
score: 522.2
NoneNo IPR availablePIRSRPIRSR630616-1PIRSR630616-1coord: 42..244
e-value: 4.8E-28
score: 95.9
NoneNo IPR availablePIRSRPIRSR037568-1PIRSR037568-1coord: 40..239
e-value: 1.6E-13
score: 46.9
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 39..232
e-value: 2.2E-11
score: 41.2
NoneNo IPR availablePANTHERPTHR24055MITOGEN-ACTIVATED PROTEIN KINASEcoord: 17..368
NoneNo IPR availablePANTHERPTHR24055:SF438MITOGEN-ACTIVATED PROTEIN KINASE 13coord: 17..368
NoneNo IPR availableCDDcd07858STKc_TEY_MAPKcoord: 31..367
e-value: 0.0
score: 678.707
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 44..68
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 160..172
IPR003527Mitogen-activated protein (MAP) kinase, conserved sitePROSITEPS01351MAPKcoord: 73..176
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 41..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0261.1MC03g0261.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010311 lateral root formation
biological_process GO:0000165 MAPK cascade
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004707 MAP kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity