MC03g0196 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0196
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	outer envelope pore protein 16, chloroplastic
Location	MC03: 3677757 .. 3679860 (+)
RNA-Seq Expression	MC03g0196
Synteny	MC03g0196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCAAGCTCGACGTCGTCATCGACATGGGCAACCCCTTCCTCAACCTCACCGTCGACGCCTTCTTCAAGATCGGATCCGTATTGACTTATTCACTTCCTCCATTTTTCTCCCTTATTCACTTCCTCTTGTTCGAATTCAAATCATTTCTCTTTTAGGTCGCTGCTACGCGTGCCGCCGCCGAGGATGCCTATCATGTTCTTAGAAAAGGTTGTATCCCTATTCCCTATTCCCTCGTCTTTGCTTTTGCTGCCGAATTTAATTGAAGCCATTGTTTGGATTCTGCAGGAACGATTTCCAGTCACAATTTCGAGCACACGGTTAGTATCTCTTGCTTGCTTCATCTATCCAGTTTTGAAGTCGCTCTGTTTGCTGAAATTTTCGCAAGTGCATAATTAGTGAAACAGATTTCTGATGTAGAGATCACAAATTACTTGTGGTAGTTTCTGTTGGCAACTCTGGTTTTATATTTGAACTGAGATTTGAAACTCTCGTTGGGTAAATGATTATATGCTGTTTTGGAAAAAATAATGTGAAATCACACACCACCAGGCCCTGAAAGTTTGAGCGAACTAATAGGGGTTTGAGCTTAAGGTGTCCTGTTTTTGAAATTAATTTTAGACCCAAGAGGTTGAATTAGCAGGCTAAGGTAAAGTTTGGTCTTCTGATCTGTACTGAGCCAGAAAAGAAAAATAAAGAACCTTGAAATTGTTGTCTCTCTCACTCAGCTTTTTTCTTCAGTTCATTAATTTCAACTTCTGTTGCAGTTGAAGAAAATGTGTAAAGAAGGTGCATACTGGGGTAAGTATGGATCACATTATTCCATAACTCGGGGAAGTAGTTTACTTAGTGGATGAGTTTCAGAATCTGTAAACCTTCTGCTAGTACCATTTGATAGTGATTTTATTTTTAGGTTATTGTTTTTGTATGTTAAGCTTATAAAGACCATACATATCTATAAGCCTATGTTTTTCTTTTTTTAATTTCCACTTTTAAAGAACACTATATTTCAAAAACAAAAAACATGTTTTTGAACTTCTTCTTTAAGATTTTTTAATTTGTCTATAATTTCAGAAACAATTTTGGTAGTCAGTTTGAAGGCGCAGAAACAAAAAGTTAATTAATAAGCATAGTTTTCAAATCAAACAGACCTTTCACTTTCACTTTTGGTTCATTTGCATGAATAAGGAACTATTTTCTCATCCGAACACAGGAACCGTAGCTGGAGTTTATGTTGGTATGGAGTATGGGGTAGAAAGGATCCGTGGCACAAGAGATTGGGTATGTTTGCCATAAATTCAAAGTTACTATGTAAATCAAGTTTGAGTTATTCTTTTAGATTATCACTTCCAGCTTTTCAAGTTTGGGAAAAATTTCTAATCCTACTGTACTCCAGTTCATTGTCATTCACGTCATAATTATTTTAAAATGAATAGAGGTACTCCACTCTAACAACGTTAAAAATTATTTAAGAAGGAAAATTTAGTGTGAACGGAGGGAATAGGTACTACTAGGAGCACTAGAAAATTTCTCTCGAGTTTGCTCGGGAATATTTTCACAATGTGTTTTCTGTATTGTTGAATGTATTAATCCTAGAATGGAAAAGAACTCCTGTTTGTTTCTATTTGATTTCAAACAAGCCAGAAACAAAATCTATTAAGATCCTAGGTAAATTGTCAATATTTCTTACAATTGTCCTCTTGGCTCTTATGAAAGTGATTATATCATTACTTACAACCATGTAACAGAGAAACAATGGTAGTACTCTACTGGTTTTCTAACTCAGGCTTAGTTATCTGTAATATTTCAAACAACGAGTTCTCGACGAAGTATGAAGTCACCAAATTATAAAAAAAAATCTACAGTTGCTTCCTATTTGTTTGAGCATAATATTACCAATTGATTCATTTGTCAATTTTTGAAGTTGAGAAATTTAATTGTGGACACAAATGTTTAAACATGTTCCAGAAAAATGCTATGATTGGAGGTGCTTTGACGGGGGCTCTGGTATCAGCAGCGAGCAGCAACAACCGAGACAAAATCGCCATCGATGCGATTACAGGAGGGGCTGTTGCAACCGCTGCGGAGTTCCTAAACTATCTTACT

mRNA sequence

CCCAAGCTCGACGTCGTCATCGACATGGGCAACCCCTTCCTCAACCTCACCGTCGACGCCTTCTTCAAGATCGGATCCGTCGCTGCTACGCGTGCCGCCGCCGAGGATGCCTATCATGTTCTTAGAAAAGGAACGATTTCCAGTCACAATTTCGAGCACACGTTGAAGAAAATGTGTAAAGAAGGTGCATACTGGGGAACCGTAGCTGGAGTTTATGTTGGTATGGAGTATGGGGTAGAAAGGATCCGTGGCACAAGAGATTGGAAAAATGCTATGATTGGAGGTGCTTTGACGGGGGCTCTGGTATCAGCAGCGAGCAGCAACAACCGAGACAAAATCGCCATCGATGCGATTACAGGAGGGGCTGTTGCAACCGCTGCGGAGTTCCTAAACTATCTTACT

Coding sequence (CDS)

CCCAAGCTCGACGTCGTCATCGACATGGGCAACCCCTTCCTCAACCTCACCGTCGACGCCTTCTTCAAGATCGGATCCGTCGCTGCTACGCGTGCCGCCGCCGAGGATGCCTATCATGTTCTTAGAAAAGGAACGATTTCCAGTCACAATTTCGAGCACACGTTGAAGAAAATGTGTAAAGAAGGTGCATACTGGGGAACCGTAGCTGGAGTTTATGTTGGTATGGAGTATGGGGTAGAAAGGATCCGTGGCACAAGAGATTGGAAAAATGCTATGATTGGAGGTGCTTTGACGGGGGCTCTGGTATCAGCAGCGAGCAGCAACAACCGAGACAAAATCGCCATCGATGCGATTACAGGAGGGGCTGTTGCAACCGCTGCGGAGTTCCTAAACTATCTTACT

Protein sequence

PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCKEGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITGGAVATAAEFLNYLT

Homology

[Close]

BLAST of MC03g0196 vs. ExPASy Swiss-Prot
Match: Q41050 (Outer envelope pore protein 16, chloroplastic OS=Pisum sativum OX=3888 GN=OEP16 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 3.3e-60
Identity = 109/134 (81.34%), Postives = 125/134 (93.28%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PKLDVVIDMGNPFLNLTVD F KIG+VAATR+ AED +H++RKG+ISS++FE +LKKMCK
Sbjct: 13  PKLDVVIDMGNPFLNLTVDGFLKIGAVAATRSVAEDTFHIIRKGSISSNDFEKSLKKMCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWG +AGVYVGMEYGVERIRGTRDWKNAM GGA+TGALVSAAS+N +DKIA+DAITG
Sbjct: 73  EGAYWGAIAGVYVGMEYGVERIRGTRDWKNAMFGGAVTGALVSAASNNKKDKIAVDAITG 132

Query: 121 GAVATAAEFLNYLT 135
            A+ATAAEF+NYLT
Sbjct: 133 AAIATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. ExPASy Swiss-Prot
Match: Q9ZV24 (Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP161 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 2.3e-45
Identity = 82/131 (62.60%), Postives = 101/131 (77.10%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PKL V +DMGNPFLNLTVDAF KIG+V  T++ AED Y  + KG++S    EH LKK+CK
Sbjct: 13  PKLSVAVDMGNPFLNLTVDAFLKIGAVGVTKSLAEDTYKAIDKGSLSKSTLEHALKKLCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EG YWG   GVY+G EYG+ERIRG+RDWKNAM+ GA TGA++SA     +D I IDAI G
Sbjct: 73  EGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATGAVLSAVGKKGKDTIVIDAILG 132

Query: 121 GAVATAAEFLN 132
           GA+ATA++F+N
Sbjct: 133 GALATASQFVN 143

[Close]

BLAST of MC03g0196 vs. ExPASy Swiss-Prot
Match: Q0WMZ5 (Outer envelope pore protein 16-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP162 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.6e-14
Identity = 47/155 (30.32%), Postives = 76/155 (49.03%), Query Frame = 0


Query: 6   VIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGT-------------------- 65
           + D+G+P LN   D+F K   V A +A + +AY  +  G                     
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHR 80

Query: 66  ------ISSHNFEHTLKKMCKEGAYWGTVAGVYVGMEYGVERIR-GTRDWKNAMIGGALT 125
                  SS + +  +K   KE   WG  AG+Y G+ YG+  +R G  DW+N+ + GALT
Sbjct: 81  FPNLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALT 140

Query: 126 GALVSAASS--NNRDKIAIDAITGGAVATAAEFLN 132
           GA ++  +S   + +++   A+TG A++TAA  L+
Sbjct: 141 GAAMAMTTSERTSHEQVVQSALTGAAISTAANLLS 175

[Close]

BLAST of MC03g0196 vs. ExPASy Swiss-Prot
Match: Q9LZH8 (Outer envelope pore protein 16-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP164 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 1.4e-05
Identity = 32/100 (32.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 12  PFLNLTVDAFFKI---GSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCKEGAYWGTV 71
           P  +LTV++  ++   G +    A   DA  +   G +S  +F    K + + G   G V
Sbjct: 10  PCSSLTVESVLRVATAGGLYGLCAGPRDARKIGLSG-VSQASF--VAKSIGRFGFQCGLV 69

Query: 72  AGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSN 109
           +GV+     G++R RG  DW NA++GGA+ GA V+ ++ N
Sbjct: 70  SGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAVAISTRN 106

[Close]

BLAST of MC03g0196 vs. NCBI nr
Match: XP_022158248.1 (outer envelope pore protein 16, chloroplastic [Momordica charantia])

HSP 1 Score: 267 bits (682), Expect = 5.26e-90
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK
Sbjct: 2   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 61

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG
Sbjct: 62  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 121

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEFLNYLT
Sbjct: 122 GAVATAAEFLNYLT 135

[Close]

BLAST of MC03g0196 vs. NCBI nr
Match: XP_038893818.1 (outer envelope pore protein 16, chloroplastic [Benincasa hispida])

HSP 1 Score: 253 bits (646), Expect = 2.36e-84
Identity = 124/134 (92.54%), Postives = 131/134 (97.76%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTIS+HNFE+TLKKMCK
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISTHNFENTLKKMCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVER+RGTRDWKNAMIGGALTGALVSAAS+NNRDKI IDAITG
Sbjct: 73  EGAYWGTVAGVYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAASNNNRDKIVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. NCBI nr
Match: XP_022964801.1 (outer envelope pore protein 16, chloroplastic [Cucurbita moschata] >XP_022970582.1 outer envelope pore protein 16, chloroplastic [Cucurbita maxima] >XP_023519566.1 outer envelope pore protein 16, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6583432.1 Outer envelope pore protein 16, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 249 bits (637), Expect = 5.56e-83
Identity = 123/134 (91.79%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTISSHNFE+TLKKMCK
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISSHNFENTLKKMCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVERIRG RDWKNAMIGGALTGA+VSAAS++NRDKI IDAITG
Sbjct: 73  EGAYWGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. NCBI nr
Match: XP_008457483.1 (PREDICTED: outer envelope pore protein 16, chloroplastic [Cucumis melo] >TYJ97413.1 outer envelope pore protein 16 [Cucumis melo var. makuwa])

HSP 1 Score: 247 bits (630), Expect = 6.49e-82
Identity = 120/134 (89.55%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTIS+HNFE+TLKKMC 
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISTHNFENTLKKMCT 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWG VAG YVGMEYGVER+RGTRDWKNAMIGGALTGALVSAAS+NNRDK+ IDAITG
Sbjct: 73  EGAYWGAVAGAYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAASNNNRDKVVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. NCBI nr
Match: KAE8023298.1 (hypothetical protein FH972_009013 [Carpinus fangiana])

HSP 1 Score: 245 bits (625), Expect = 3.75e-81
Identity = 116/134 (86.57%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DV IDMGNPFLNLTVD F KIG+VAATRA AEDAYH ++KG+IS+HNFEHTLKKMCK
Sbjct: 13  PKVDVAIDMGNPFLNLTVDGFLKIGTVAATRAVAEDAYHAVKKGSISTHNFEHTLKKMCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGA+TGALVSAAS+NN+DK+ +DAITG
Sbjct: 73  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGAVTGALVSAASNNNKDKVVVDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GA+ATAAEFLNYLT
Sbjct: 133 GAIATAAEFLNYLT 146

[Close]

BLAST of MC03g0196 vs. ExPASy TrEMBL
Match: A0A6J1DVJ9 (outer envelope pore protein 16, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111024780 PE=4 SV=1)

HSP 1 Score: 267 bits (682), Expect = 2.55e-90
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK
Sbjct: 2   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 61

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG
Sbjct: 62  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 121

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEFLNYLT
Sbjct: 122 GAVATAAEFLNYLT 135

[Close]

BLAST of MC03g0196 vs. ExPASy TrEMBL
Match: A0A6J1HZI5 (outer envelope pore protein 16, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111469516 PE=4 SV=1)

HSP 1 Score: 249 bits (637), Expect = 2.69e-83
Identity = 123/134 (91.79%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTISSHNFE+TLKKMCK
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISSHNFENTLKKMCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVERIRG RDWKNAMIGGALTGA+VSAAS++NRDKI IDAITG
Sbjct: 73  EGAYWGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. ExPASy TrEMBL
Match: A0A6J1HJY9 (outer envelope pore protein 16, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111464797 PE=4 SV=1)

HSP 1 Score: 249 bits (637), Expect = 2.69e-83
Identity = 123/134 (91.79%), Postives = 130/134 (97.01%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTISSHNFE+TLKKMCK
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISSHNFENTLKKMCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWGTVAGVYVGMEYGVERIRG RDWKNAMIGGALTGA+VSAAS++NRDKI IDAITG
Sbjct: 73  EGAYWGTVAGVYVGMEYGVERIRGRRDWKNAMIGGALTGAMVSAASNSNRDKIVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. ExPASy TrEMBL
Match: A0A5D3BDN0 (Outer envelope pore protein 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002350 PE=4 SV=1)

HSP 1 Score: 247 bits (630), Expect = 3.14e-82
Identity = 120/134 (89.55%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTIS+HNFE+TLKKMC 
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISTHNFENTLKKMCT 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWG VAG YVGMEYGVER+RGTRDWKNAMIGGALTGALVSAAS+NNRDK+ IDAITG
Sbjct: 73  EGAYWGAVAGAYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAASNNNRDKVVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. ExPASy TrEMBL
Match: A0A1S3C572 (outer envelope pore protein 16, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497163 PE=4 SV=1)

HSP 1 Score: 247 bits (630), Expect = 3.14e-82
Identity = 120/134 (89.55%), Postives = 128/134 (95.52%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PK+DVVIDMGNPFLNLTVD F KIGSVAATRAAAEDAYHV+RKGTIS+HNFE+TLKKMC 
Sbjct: 13  PKVDVVIDMGNPFLNLTVDGFLKIGSVAATRAAAEDAYHVVRKGTISTHNFENTLKKMCT 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EGAYWG VAG YVGMEYGVER+RGTRDWKNAMIGGALTGALVSAAS+NNRDK+ IDAITG
Sbjct: 73  EGAYWGAVAGAYVGMEYGVERVRGTRDWKNAMIGGALTGALVSAASNNNRDKVVIDAITG 132

Query: 121 GAVATAAEFLNYLT 134
           GAVATAAEF+NYLT
Sbjct: 133 GAVATAAEFINYLT 146

[Close]

BLAST of MC03g0196 vs. TAIR 10
Match: AT2G28900.1 (outer plastid envelope protein 16-1 )

HSP 1 Score: 183.0 bits (463), Expect = 1.6e-46
Identity = 82/131 (62.60%), Postives = 101/131 (77.10%), Query Frame = 0


Query: 1   PKLDVVIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCK 60
           PKL V +DMGNPFLNLTVDAF KIG+V  T++ AED Y  + KG++S    EH LKK+CK
Sbjct: 13  PKLSVAVDMGNPFLNLTVDAFLKIGAVGVTKSLAEDTYKAIDKGSLSKSTLEHALKKLCK 72

Query: 61  EGAYWGTVAGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSNNRDKIAIDAITG 120
           EG YWG   GVY+G EYG+ERIRG+RDWKNAM+ GA TGA++SA     +D I IDAI G
Sbjct: 73  EGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATGAVLSAVGKKGKDTIVIDAILG 132

Query: 121 GAVATAAEFLN 132
           GA+ATA++F+N
Sbjct: 133 GALATASQFVN 143

[Close]

BLAST of MC03g0196 vs. TAIR 10
Match: AT4G16160.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-15
Identity = 47/153 (30.72%), Postives = 76/153 (49.67%), Query Frame = 0


Query: 6   VIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKG--------------------- 65
           + D+G+P LN   D+F K   V A +A + +AY  +  G                     
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGFDSNNVGPPSEITGNKKHRFP 80

Query: 66  ---TISSHNFEHTLKKMCKEGAYWGTVAGVYVGMEYGVERIR-GTRDWKNAMIGGALTGA 125
                SS + +  +K   KE   WG  AG+Y G+ YG+  +R G  DW+N+ + GALTGA
Sbjct: 81  NLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALTGA 140

Query: 126 LVSAASS--NNRDKIAIDAITGGAVATAAEFLN 132
            ++  +S   + +++   A+TG A++TAA  L+
Sbjct: 141 AMAMTTSERTSHEQVVQSALTGAAISTAANLLS 173

[Close]

BLAST of MC03g0196 vs. TAIR 10
Match: AT4G16160.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.1e-15
Identity = 47/155 (30.32%), Postives = 76/155 (49.03%), Query Frame = 0


Query: 6   VIDMGNPFLNLTVDAFFKIGSVAATRAAAEDAYHVLRKGT-------------------- 65
           + D+G+P LN   D+F K   V A +A + +AY  +  G                     
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHR 80

Query: 66  ------ISSHNFEHTLKKMCKEGAYWGTVAGVYVGMEYGVERIR-GTRDWKNAMIGGALT 125
                  SS + +  +K   KE   WG  AG+Y G+ YG+  +R G  DW+N+ + GALT
Sbjct: 81  FPNLRGESSKSLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALT 140

Query: 126 GALVSAASS--NNRDKIAIDAITGGAVATAAEFLN 132
           GA ++  +S   + +++   A+TG A++TAA  L+
Sbjct: 141 GAAMAMTTSERTSHEQVVQSALTGAAISTAANLLS 175

[Close]

BLAST of MC03g0196 vs. TAIR 10
Match: AT3G62880.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 50.8 bits (120), Expect = 9.7e-07
Identity = 32/100 (32.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 12  PFLNLTVDAFFKI---GSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCKEGAYWGTV 71
           P  +LTV++  ++   G +    A   DA  +   G +S  +F    K + + G   G V
Sbjct: 10  PCSSLTVESVLRVATAGGLYGLCAGPRDARKIGLSG-VSQASF--VAKSIGRFGFQCGLV 69

Query: 72  AGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSN 109
           +GV+     G++R RG  DW NA++GGA+ GA V+ ++ N
Sbjct: 70  SGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAVAISTRN 106

[Close]

BLAST of MC03g0196 vs. TAIR 10
Match: AT3G62880.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 50.8 bits (120), Expect = 9.7e-07
Identity = 32/100 (32.00%), Postives = 53/100 (53.00%), Query Frame = 0


Query: 12  PFLNLTVDAFFKI---GSVAATRAAAEDAYHVLRKGTISSHNFEHTLKKMCKEGAYWGTV 71
           P  +LTV++  ++   G +    A   DA  +   G +S  +F    K + + G   G V
Sbjct: 10  PCSSLTVESVLRVATAGGLYGLCAGPRDARKIGLSG-VSQASF--VAKSIGRFGFQCGLV 69

Query: 72  AGVYVGMEYGVERIRGTRDWKNAMIGGALTGALVSAASSN 109
           +GV+     G++R RG  DW NA++GGA+ GA V+ ++ N
Sbjct: 70  SGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAVAISTRN 106

The following BLAST results are available for this feature:

BLAST of MC03g0196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41050	3.3e-60	81.34	Outer envelope pore protein 16, chloroplastic OS=Pisum sativum OX=3888 GN=OEP16 ...	[more]
Q9ZV24	2.3e-45	62.60	Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q0WMZ5	1.6e-14	30.32	Outer envelope pore protein 16-2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9LZH8	1.4e-05	32.00	Outer envelope pore protein 16-4, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of MC03g0196 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158248.1	5.26e-90	100.00	outer envelope pore protein 16, chloroplastic [Momordica charantia]	[more]
XP_038893818.1	2.36e-84	92.54	outer envelope pore protein 16, chloroplastic [Benincasa hispida]	[more]
XP_022964801.1	5.56e-83	91.79	outer envelope pore protein 16, chloroplastic [Cucurbita moschata] >XP_022970582...	[more]
XP_008457483.1	6.49e-82	89.55	PREDICTED: outer envelope pore protein 16, chloroplastic [Cucumis melo] >TYJ9741...	[more]
KAE8023298.1	3.75e-81	86.57	hypothetical protein FH972_009013 [Carpinus fangiana]	[more]

BLAST of MC03g0196 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVJ9	2.55e-90	100.00	outer envelope pore protein 16, chloroplastic OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1HZI5	2.69e-83	91.79	outer envelope pore protein 16, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1HJY9	2.69e-83	91.79	outer envelope pore protein 16, chloroplastic OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A5D3BDN0	3.14e-82	89.55	Outer envelope pore protein 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3C572	3.14e-82	89.55	outer envelope pore protein 16, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034...	[more]

BLAST of MC03g0196 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28900.1	1.6e-46	62.60	outer plastid envelope protein 16-1	[more]
AT4G16160.1	1.1e-15	30.72	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT4G16160.2	1.1e-15	30.32	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT3G62880.1	9.7e-07	32.00	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT3G62880.2	9.7e-07	32.00	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 45..123 e-value: 4.5E-12 score: 46.4
None	No IPR available	PANTHER	PTHR15371:SF2	OUTER ENVELOPE PORE PROTEIN 16-1, CHLOROPLASTIC	coord: 1..132
None	No IPR available	PANTHER	PTHR15371	TIM23	coord: 1..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0196.1	MC03g0196.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0003333	amino acid transmembrane transport
biological_process	GO:0045037	protein import into chloroplast stroma
cellular_component	GO:0009707	chloroplast outer membrane
molecular_function	GO:0015171	amino acid transmembrane transporter activity