MC03g0188 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0188
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC03: 3538311 .. 3538451 (+)
RNA-Seq Expression	MC03g0188
Synteny	MC03g0188

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATGACGACGAATGTGCGATTTGCCTGGGCGAGTTTGAAGAAGGGGAGAAGTGCCGGAAGATGAGTGAGTGCGGCCACATATTTCACCGGAACTGCATTGACCGGTGGCTGAGGGTGGAGCGCCACTGCCCACTTTGCCGG

mRNA sequence

GATGACGACGAATGTGCGATTTGCCTGGGCGAGTTTGAAGAAGGGGAGAAGTGCCGGAAGATGAGTGAGTGCGGCCACATATTTCACCGGAACTGCATTGACCGGTGGCTGAGGGTGGAGCGCCACTGCCCACTTTGCCGG

Coding sequence (CDS)

GATGACGACGAATGTGCGATTTGCCTGGGCGAGTTTGAAGAAGGGGAGAAGTGCCGGAAGATGAGTGAGTGCGGCCACATATTTCACCGGAACTGCATTGACCGGTGGCTGAGGGTGGAGCGCCACTGCCCACTTTGCCGG

Protein sequence

DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR

Homology

[Close]

BLAST of MC03g0188 vs. ExPASy Swiss-Prot
Match: Q9SRM0 (RING-H2 finger protein ATL66 OS=Arabidopsis thaliana OX=3702 GN=ATL66 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 7.6e-12
Identity = 26/47 (55.32%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           ++ EC ICLG FEEGEK + +  C H +H  C+DRWL+ E  CPLCR
Sbjct: 103 EEKECCICLGGFEEGEKMKVLPPCSHCYHCECVDRWLKTESSCPLCR 149

[Close]

BLAST of MC03g0188 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.8e-11
Identity = 26/47 (55.32%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           D  EC++CL EFEE E  R + +C H FH NCID WL   ++CPLCR
Sbjct: 131 DGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCR 177

[Close]

BLAST of MC03g0188 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-10
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 4   ECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           +C++CLGEF +GE  R +  C H FH+ CID WL+   +CPLCR
Sbjct: 156 DCSVCLGEFSDGESLRLLPRCSHAFHQQCIDTWLKSHSNCPLCR 199

[Close]

BLAST of MC03g0188 vs. ExPASy Swiss-Prot
Match: Q9LZJ6 (RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-10
Identity = 24/45 (53.33%), Postives = 33/45 (73.33%), Query Frame = 0


Query: 3   DECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           +EC++CL EFEE ++ R + +CGH+FH +CID W R    CPLCR
Sbjct: 111 EECSVCLSEFEEDDEGRVLPKCGHVFHVDCIDTWFRSRSSCPLCR 155

[Close]

BLAST of MC03g0188 vs. ExPASy Swiss-Prot
Match: Q9M0C3 (RING-H2 finger protein ATL14 OS=Arabidopsis thaliana OX=3702 GN=ATL14 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.4e-10
Identity = 22/45 (48.89%), Postives = 31/45 (68.89%), Query Frame = 0


Query: 3   DECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           D+C +C+  F +G+ CRK+  CGH+FHR C+D WL     CP+CR
Sbjct: 113 DDCVVCIDGFRQGQWCRKLPRCGHVFHRKCVDLWLIKVSTCPICR 157

[Close]

BLAST of MC03g0188 vs. NCBI nr
Match: XP_022158250.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 112 bits (281), Expect = 2.90e-30
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR
Sbjct: 87  DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 133

[Close]

BLAST of MC03g0188 vs. NCBI nr
Match: XP_022998666.1 (RING-H2 finger protein ATL56-like [Cucurbita maxima])

HSP 1 Score: 98.6 bits (244), Expect = 9.32e-25
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 2   DDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DDECAICL EFE+G+KCRKM++CGHIFHR CIDRWL+V+RHCPLCR
Sbjct: 89  DDECAICLDEFEDGQKCRKMNKCGHIFHRRCIDRWLKVDRHCPLCR 134

[Close]

BLAST of MC03g0188 vs. NCBI nr
Match: XP_022158251.1 (RING-H2 finger protein ATL1-like [Momordica charantia])

HSP 1 Score: 97.1 bits (240), Expect = 5.32e-24
Identity = 37/47 (78.72%), Postives = 44/47 (93.62%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DD+ECAICL +FE+GEKCRKMS+C H+FHR+CIDRWL+ ERHCPLCR
Sbjct: 89  DDEECAICLSKFEQGEKCRKMSKCDHVFHRDCIDRWLKAERHCPLCR 135

[Close]

BLAST of MC03g0188 vs. NCBI nr
Match: XP_023524567.1 (RING-H2 finger protein ATL56-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 96.3 bits (238), Expect = 7.58e-24
Identity = 37/46 (80.43%), Postives = 43/46 (93.48%), Query Frame = 0


Query: 2   DDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DDECAICL EFE+ +KCRKM++CGHIFHR CIDRWL+V+RHCPLCR
Sbjct: 89  DDECAICLDEFEDAQKCRKMNKCGHIFHRRCIDRWLKVDRHCPLCR 134

[Close]

BLAST of MC03g0188 vs. NCBI nr
Match: KAG7036928.1 (RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 95.9 bits (237), Expect = 2.11e-22
Identity = 37/46 (80.43%), Postives = 42/46 (91.30%), Query Frame = 0


Query: 2   DDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DDECAICL EFE+ +KCRKM+ CGHIFHR CIDRWL+V+RHCPLCR
Sbjct: 89  DDECAICLDEFEDAQKCRKMNNCGHIFHRRCIDRWLKVDRHCPLCR 134

[Close]

BLAST of MC03g0188 vs. ExPASy TrEMBL
Match: A0A6J1DVB5 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111024784 PE=3 SV=1)

HSP 1 Score: 112 bits (281), Expect = 1.40e-30
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR
Sbjct: 87  DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 133

[Close]

BLAST of MC03g0188 vs. ExPASy TrEMBL
Match: A0A6J1KAT3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111493250 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 4.51e-25
Identity = 38/46 (82.61%), Postives = 44/46 (95.65%), Query Frame = 0


Query: 2   DDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DDECAICL EFE+G+KCRKM++CGHIFHR CIDRWL+V+RHCPLCR
Sbjct: 89  DDECAICLDEFEDGQKCRKMNKCGHIFHRRCIDRWLKVDRHCPLCR 134

[Close]

BLAST of MC03g0188 vs. ExPASy TrEMBL
Match: A0A6J1E0E9 (RING-H2 finger protein ATL1-like OS=Momordica charantia OX=3673 GN=LOC111024786 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.58e-24
Identity = 37/47 (78.72%), Postives = 44/47 (93.62%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
           DD+ECAICL +FE+GEKCRKMS+C H+FHR+CIDRWL+ ERHCPLCR
Sbjct: 89  DDEECAICLSKFEQGEKCRKMSKCDHVFHRDCIDRWLKAERHCPLCR 135

[Close]

BLAST of MC03g0188 vs. ExPASy TrEMBL
Match: A0A5A7U2S7 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00960 PE=3 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.04e-21
Identity = 35/47 (74.47%), Postives = 39/47 (82.98%), Query Frame = 0


Query: 1  DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
          D+ ECAICL E EEGEKCRKM  CGH+FH++CIDRW  VE HCPLCR
Sbjct: 48 DEQECAICLCEMEEGEKCRKMKTCGHVFHKDCIDRWFTVELHCPLCR 94

[Close]

BLAST of MC03g0188 vs. ExPASy TrEMBL
Match: A0A5D3BFU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold54436G00010 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.40e-21
Identity = 35/47 (74.47%), Postives = 39/47 (82.98%), Query Frame = 0


Query: 1  DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 47
          D+ ECAICL E EEGEKCRKM  CGH+FH++CIDRW  VE HCPLCR
Sbjct: 46 DEQECAICLCEIEEGEKCRKMKMCGHVFHKDCIDRWFTVELHCPLCR 92

[Close]

BLAST of MC03g0188 vs. TAIR 10
Match: AT3G11110.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 5.4e-13
Identity = 26/47 (55.32%), Postives = 33/47 (70.21%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           ++ EC ICLG FEEGEK + +  C H +H  C+DRWL+ E  CPLCR
Sbjct: 103 EEKECCICLGGFEEGEKMKVLPPCSHCYHCECVDRWLKTESSCPLCR 149

[Close]

BLAST of MC03g0188 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 2.7e-12
Identity = 26/47 (55.32%), Postives = 32/47 (68.09%), Query Frame = 0


Query: 1   DDDECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           D  EC++CL EFEE E  R + +C H FH NCID WL   ++CPLCR
Sbjct: 131 DGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCR 177

[Close]

BLAST of MC03g0188 vs. TAIR 10
Match: AT3G20395.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 6.0e-12
Identity = 25/43 (58.14%), Postives = 32/43 (74.42%), Query Frame = 0


Query: 5   CAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           C+ICL ++EEGE  RK++ CGH FH NCID WL  +  CP+CR
Sbjct: 171 CSICLQDWEEGEVGRKLARCGHTFHMNCIDEWLLRQETCPICR 213

[Close]

BLAST of MC03g0188 vs. TAIR 10
Match: AT3G62690.1 (AtL5 )

HSP 1 Score: 66.2 bits (160), Expect = 7.8e-12
Identity = 24/45 (53.33%), Postives = 33/45 (73.33%), Query Frame = 0


Query: 3   DECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           +EC++CL EFEE ++ R + +CGH+FH +CID W R    CPLCR
Sbjct: 111 EECSVCLSEFEEDDEGRVLPKCGHVFHVDCIDTWFRSRSSCPLCR 155

[Close]

BLAST of MC03g0188 vs. TAIR 10
Match: AT4G30370.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-11
Identity = 22/45 (48.89%), Postives = 31/45 (68.89%), Query Frame = 0


Query: 3   DECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 48
           D+C +C+  F +G+ CRK+  CGH+FHR C+D WL     CP+CR
Sbjct: 113 DDCVVCIDGFRQGQWCRKLPRCGHVFHRKCVDLWLIKVSTCPICR 157

The following BLAST results are available for this feature:

BLAST of MC03g0188 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRM0	7.6e-12	55.32	RING-H2 finger protein ATL66 OS=Arabidopsis thaliana OX=3702 GN=ATL66 PE=2 SV=1	[more]
Q9LX93	3.8e-11	55.32	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q7XLY8	1.1e-10	52.27	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9LZJ6	1.1e-10	53.33	RING-H2 finger protein ATL5 OS=Arabidopsis thaliana OX=3702 GN=ATL5 PE=2 SV=1	[more]
Q9M0C3	1.4e-10	48.89	RING-H2 finger protein ATL14 OS=Arabidopsis thaliana OX=3702 GN=ATL14 PE=2 SV=1	[more]

BLAST of MC03g0188 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158250.1	2.90e-30	100.00	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
XP_022998666.1	9.32e-25	82.61	RING-H2 finger protein ATL56-like [Cucurbita maxima]	[more]
XP_022158251.1	5.32e-24	78.72	RING-H2 finger protein ATL1-like [Momordica charantia]	[more]
XP_023524567.1	7.58e-24	80.43	RING-H2 finger protein ATL56-like [Cucurbita pepo subsp. pepo]	[more]
KAG7036928.1	2.11e-22	80.43	RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]

BLAST of MC03g0188 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVB5	1.40e-30	100.00	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102478...	[more]
A0A6J1KAT3	4.51e-25	82.61	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111493250 P...	[more]
A0A6J1E0E9	2.58e-24	78.72	RING-H2 finger protein ATL1-like OS=Momordica charantia OX=3673 GN=LOC111024786 ...	[more]
A0A5A7U2S7	1.04e-21	74.47	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A5D3BFU3	1.40e-21	74.47	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of MC03g0188 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11110.1	5.4e-13	55.32	RING/U-box superfamily protein	[more]
AT5G10380.1	2.7e-12	55.32	RING/U-box superfamily protein	[more]
AT3G20395.1	6.0e-12	58.14	RING/U-box superfamily protein	[more]
AT3G62690.1	7.8e-12	53.33	AtL5	[more]
AT4G30370.1	1.0e-11	48.89	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 5..46 e-value: 1.1E-6 score: 38.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 3..47 e-value: 2.8E-16 score: 59.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 5..47 score: 13.300693
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..47 e-value: 1.4E-19 score: 71.4
None	No IPR available	PANTHER	PTHR14155	RING FINGER DOMAIN-CONTAINING	coord: 3..47
None	No IPR available	PANTHER	PTHR14155:SF550	RING-H2 FINGER PROTEIN ATL40	coord: 3..47
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 1..47

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0188.1	MC03g0188.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane