MC03g0186 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0186
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC03: 3532517 .. 3532645 (+)
RNA-Seq Expression	MC03g0186
Synteny	MC03g0186

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAATGTGTGATTTGCCTGTGCGGATATGAAGAAGGGGAGAAGTGGCTGAAGATGAAGTGCGGCCATATATTTCACCGGAGCTGTATTGAGCGGTGGCTGATGGTGGGCCGCCACTGCCCGCTTTGCCGG

mRNA sequence

GAATGTGTGATTTGCCTGTGCGGATATGAAGAAGGGGAGAAGTGGCTGAAGATGAAGTGCGGCCATATATTTCACCGGAGCTGTATTGAGCGGTGGCTGATGGTGGGCCGCCACTGCCCGCTTTGCCGG

Coding sequence (CDS)

GAATGTGTGATTTGCCTGTGCGGATATGAAGAAGGGGAGAAGTGGCTGAAGATGAAGTGCGGCCATATATTTCACCGGAGCTGTATTGAGCGGTGGCTGATGGTGGGCCGCCACTGCCCGCTTTGCCGG

Protein sequence

ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR

Homology

[Close]

BLAST of MC03g0186 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.5e-09
Identity = 21/43 (48.84%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICL  YE+G +  ++ CGH FH SC+++WL +   CPLC+
Sbjct: 352 ECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLCK 394

[Close]

BLAST of MC03g0186 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 5.5e-09
Identity = 21/43 (48.84%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICLC YE+G +  ++ C H FH +CI++WL +   CPLC+
Sbjct: 337 ECCICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCK 379

[Close]

BLAST of MC03g0186 vs. ExPASy Swiss-Prot
Match: O00237 (E3 ubiquitin-protein ligase RNF103 OS=Homo sapiens OX=9606 GN=RNF103 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-08
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRH-CPLCR 44
           ECV+CL  +E G   + + CGH+FH++CI  WL  GRH CP+CR
Sbjct: 620 ECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCR 663

[Close]

BLAST of MC03g0186 vs. ExPASy Swiss-Prot
Match: Q9R1W3 (E3 ubiquitin-protein ligase RNF103 OS=Mus musculus OX=10090 GN=Rnf103 PE=2 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-08
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRH-CPLCR 44
           ECV+CL  +E G   + + CGH+FH++CI  WL  GRH CP+CR
Sbjct: 618 ECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCR 661

[Close]

BLAST of MC03g0186 vs. ExPASy Swiss-Prot
Match: Q9EPZ8 (E3 ubiquitin-protein ligase RNF103 OS=Rattus norvegicus OX=10116 GN=Rnf103 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.1e-08
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRH-CPLCR 44
           ECV+CL  +E G   + + CGH+FH++CI  WL  GRH CP+CR
Sbjct: 617 ECVVCLENFENGCLLMGLPCGHVFHQNCIVMWLAGGRHCCPVCR 660

[Close]

BLAST of MC03g0186 vs. NCBI nr
Match: XP_022158252.1 (E3 ubiquitin-protein ligase RHA2B-like [Momordica charantia])

HSP 1 Score: 105 bits (261), Expect = 2.50e-27
Identity = 43/43 (100.00%), Postives = 43/43 (100.00%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 43
           ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR
Sbjct: 79  ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 121

[Close]

BLAST of MC03g0186 vs. NCBI nr
Match: XP_011648512.2 (RING-H2 finger protein ATL79 [Cucumis sativus])

HSP 1 Score: 73.2 bits (178), Expect = 8.69e-15
Identity = 30/44 (68.18%), Postives = 34/44 (77.27%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMK-CGHIFHRSCIERWLMVGRHCPLCR 43
           ECVICLC  EEGEK  KMK CGH+FH+ CI+RW  V  HCP+CR
Sbjct: 77  ECVICLCEIEEGEKCRKMKTCGHVFHKDCIDRWFKVNGHCPICR 120

[Close]

BLAST of MC03g0186 vs. NCBI nr
Match: XP_022158250.1 (E3 ubiquitin-protein ligase RING1-like [Momordica charantia])

HSP 1 Score: 73.2 bits (178), Expect = 9.30e-15
Identity = 32/44 (72.73%), Postives = 36/44 (81.82%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKM-KCGHIFHRSCIERWLMVGRHCPLCR 43
           EC ICL  +EEGEK  KM +CGHIFHR+CI+RWL V RHCPLCR
Sbjct: 90  ECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 133

[Close]

BLAST of MC03g0186 vs. NCBI nr
Match: XP_008457568.1 (PREDICTED: RING-H2 finger protein ATL64-like [Cucumis melo] >TYJ98123.1 RING-H2 finger protein ATL64-like [Cucumis melo var. makuwa])

HSP 1 Score: 71.6 bits (174), Expect = 1.64e-14
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 1  ECVICLCGYEEGEKWLKMK-CGHIFHRSCIERWLMVGRHCPLCR 43
          EC ICLC  EEGEK  KMK CGH+FH+ CI+RW  V  HCPLCR
Sbjct: 49 ECAICLCEIEEGEKCRKMKMCGHVFHKDCIDRWFTVELHCPLCR 92

[Close]

BLAST of MC03g0186 vs. NCBI nr
Match: KAA0050142.1 (RING-H2 finger protein ATL63-like [Cucumis melo var. makuwa] >TYK06399.1 RING-H2 finger protein ATL63-like [Cucumis melo var. makuwa])

HSP 1 Score: 71.6 bits (174), Expect = 1.72e-14
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 1  ECVICLCGYEEGEKWLKMK-CGHIFHRSCIERWLMVGRHCPLCR 43
          EC ICLC  EEGEK  KMK CGH+FH+ CI+RW  V  HCPLCR
Sbjct: 51 ECAICLCEMEEGEKCRKMKTCGHVFHKDCIDRWFTVELHCPLCR 94

[Close]

BLAST of MC03g0186 vs. ExPASy TrEMBL
Match: A0A6J1DWR6 (E3 ubiquitin-protein ligase RHA2B-like OS=Momordica charantia OX=3673 GN=LOC111024787 PE=4 SV=1)

HSP 1 Score: 105 bits (261), Expect = 1.21e-27
Identity = 43/43 (100.00%), Postives = 43/43 (100.00%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 43
           ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR
Sbjct: 79  ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 121

[Close]

BLAST of MC03g0186 vs. ExPASy TrEMBL
Match: A0A0A0LUU6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G512470 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.58e-15
Identity = 30/44 (68.18%), Postives = 34/44 (77.27%), Query Frame = 0


Query: 1  ECVICLCGYEEGEKWLKMK-CGHIFHRSCIERWLMVGRHCPLCR 43
          ECVICLC  EEGEK  KMK CGH+FH+ CI+RW  V  HCP+CR
Sbjct: 56 ECVICLCEIEEGEKCRKMKTCGHVFHKDCIDRWFKVNGHCPICR 99

[Close]

BLAST of MC03g0186 vs. ExPASy TrEMBL
Match: A0A6J1DVB5 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111024784 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 4.50e-15
Identity = 32/44 (72.73%), Postives = 36/44 (81.82%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKM-KCGHIFHRSCIERWLMVGRHCPLCR 43
           EC ICL  +EEGEK  KM +CGHIFHR+CI+RWL V RHCPLCR
Sbjct: 90  ECAICLGEFEEGEKCRKMSECGHIFHRNCIDRWLRVERHCPLCR 133

[Close]

BLAST of MC03g0186 vs. ExPASy TrEMBL
Match: A0A5D3BFU3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold54436G00010 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.94e-15
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 1  ECVICLCGYEEGEKWLKMK-CGHIFHRSCIERWLMVGRHCPLCR 43
          EC ICLC  EEGEK  KMK CGH+FH+ CI+RW  V  HCPLCR
Sbjct: 49 ECAICLCEIEEGEKCRKMKMCGHVFHKDCIDRWFTVELHCPLCR 92

[Close]

BLAST of MC03g0186 vs. ExPASy TrEMBL
Match: A0A1S3C5E4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103497234 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 7.94e-15
Identity = 30/44 (68.18%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 1  ECVICLCGYEEGEKWLKMK-CGHIFHRSCIERWLMVGRHCPLCR 43
          EC ICLC  EEGEK  KMK CGH+FH+ CI+RW  V  HCPLCR
Sbjct: 49 ECAICLCEIEEGEKCRKMKMCGHVFHKDCIDRWFTVELHCPLCR 92

[Close]

BLAST of MC03g0186 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 60.5 bits (145), Expect = 3.9e-10
Identity = 21/43 (48.84%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICL  YE+G +  ++ CGH FH SC+++WL +   CPLC+
Sbjct: 352 ECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLCK 394

[Close]

BLAST of MC03g0186 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 60.5 bits (145), Expect = 3.9e-10
Identity = 21/43 (48.84%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICL  YE+G +  ++ CGH FH SC+++WL +   CPLC+
Sbjct: 281 ECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLCK 323

[Close]

BLAST of MC03g0186 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 60.5 bits (145), Expect = 3.9e-10
Identity = 21/43 (48.84%), Postives = 30/43 (69.77%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICLC YE+G +  ++ C H FH +CI++WL +   CPLC+
Sbjct: 337 ECCICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCK 379

[Close]

BLAST of MC03g0186 vs. TAIR 10
Match: AT3G61180.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-09
Identity = 20/43 (46.51%), Postives = 29/43 (67.44%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKMKCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICLC YE+G +  ++ C H FH  C+++WL +   CPLC+
Sbjct: 322 ECSICLCAYEDGVELRELPCRHHFHSLCVDKWLRINATCPLCK 364

[Close]

BLAST of MC03g0186 vs. TAIR 10
Match: AT3G11110.1 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 5.7e-09
Identity = 23/44 (52.27%), Postives = 28/44 (63.64%), Query Frame = 0


Query: 1   ECVICLCGYEEGEKWLKM-KCGHIFHRSCIERWLMVGRHCPLCR 44
           EC ICL G+EEGEK   +  C H +H  C++RWL     CPLCR
Sbjct: 106 ECCICLGGFEEGEKMKVLPPCSHCYHCECVDRWLKTESSCPLCR 149

The following BLAST results are available for this feature:

BLAST of MC03g0186 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LN71	5.5e-09	48.84	E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127...	[more]
Q93Z92	5.5e-09	48.84	E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116...	[more]
O00237	2.1e-08	52.27	E3 ubiquitin-protein ligase RNF103 OS=Homo sapiens OX=9606 GN=RNF103 PE=1 SV=1	[more]
Q9R1W3	2.1e-08	52.27	E3 ubiquitin-protein ligase RNF103 OS=Mus musculus OX=10090 GN=Rnf103 PE=2 SV=2	[more]
Q9EPZ8	2.1e-08	52.27	E3 ubiquitin-protein ligase RNF103 OS=Rattus norvegicus OX=10116 GN=Rnf103 PE=2 ...	[more]

BLAST of MC03g0186 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158252.1	2.50e-27	100.00	E3 ubiquitin-protein ligase RHA2B-like [Momordica charantia]	[more]
XP_011648512.2	8.69e-15	68.18	RING-H2 finger protein ATL79 [Cucumis sativus]	[more]
XP_022158250.1	9.30e-15	72.73	E3 ubiquitin-protein ligase RING1-like [Momordica charantia]	[more]
XP_008457568.1	1.64e-14	68.18	PREDICTED: RING-H2 finger protein ATL64-like [Cucumis melo] >TYJ98123.1 RING-H2 ...	[more]
KAA0050142.1	1.72e-14	68.18	RING-H2 finger protein ATL63-like [Cucumis melo var. makuwa] >TYK06399.1 RING-H2...	[more]

BLAST of MC03g0186 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWR6	1.21e-27	100.00	E3 ubiquitin-protein ligase RHA2B-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0LUU6	2.58e-15	68.18	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G512470 PE...	[more]
A0A6J1DVB5	4.50e-15	72.73	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102478...	[more]
A0A5D3BFU3	7.94e-15	68.18	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C5E4	7.94e-15	68.18	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103497234 PE=3 ...	[more]

BLAST of MC03g0186 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12760.1	3.9e-10	48.84	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT1G12760.2	3.9e-10	48.84	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT4G11680.1	3.9e-10	48.84	Zinc finger, C3HC4 type (RING finger) family protein	[more]
AT3G61180.1	1.1e-09	46.51	RING/U-box superfamily protein	[more]
AT3G11110.1	5.7e-09	52.27	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 2..42 e-value: 7.6E-6 score: 35.4
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 1..43 e-value: 4.1E-15 score: 55.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 2..43 score: 12.445019
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..43 e-value: 1.3E-16 score: 61.8
None	No IPR available	PANTHER	PTHR45931	SI:CH211-59O9.10	coord: 1..43
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 1..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0186.1	MC03g0186.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane