MC03g0155 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC03g0155
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionTropinone reductase-like protein
LocationMC03: 2728211 .. 2730844 (-)
RNA-Seq ExpressionMC03g0155
SyntenyMC03g0155
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCACCCCAATCGATGATCGGTGGTCTCTTCGTGGAGCTACGGCCCTCGTCACCGGCGGCACTCGCGGAATCGGGTAAGATTTTTCTATCTATGAACAAATAGAGGCGATTTCCGTTTTGTTCCTCTGATGTTTGTTGAGAATTTGGAATTGGATTCTTGTTTAGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCAAGAGTTCATACGTGTTCTCGGAATTTGAGTGAGTTGCAACAATGTTTGAGGGATTGGAAGAATTTGGAGTTTGGAGTTTCTGGGTCGGTCTGCGATGTGTCGGTTCGAGCTCAGAGAGAGGAGCTAATGGAAACAGTGGCTGCCAATTTTGATGGGAAGCTCAACATTCTTGTAAGGTGCACCATTTCTTATGCTTTGCTCTGCTTTAGAATAAGTTGCAGATTACTTTGTTGGGTTGTTTTCCTTCCAATCTCATCTCTTATAATTATAAAACTATTGGAAGTAACCTTCTAGCTGGAACTATCATTAGATGTATGATCAAAATAATATTTCTGCGCTTGGAATGGTCGGATAATTGTCCTTTTAAGAAGGGGGGATACCAATGTTTTTTCCTTTTTTTTTTTTTCTGAAGAGGCATGCCAAAGCGTCGAGGTAGTATGAGGCGTAAAGACTTGAACTCGTGGTGCATTATTCTCATAGAAGTGTAAGCTAAAGAAAGTGTAAGACTTGAAGTCAAAGATTTGAGTCCTGCCTTGAGGCGAGTTTAAGGTGTAAGCTTCAAAAGCCTCTTAAAGTATTAGATGAGACAATAAAGAGGCGAGGAAGGCGGTTTCGTTCAGTAGAATGTAGATCATTTAGCTAAAAGATCTGTAGTTTGCTGTTGCTTGAATAAGAAAAATGGAGTGGTTACATAATCAAAACTTCAGACAGCGAGCTCCGAGAAGAGGGAATAACTAGATTCTAAATATCCTCAAAACTTTTCTGTCCTTCAACGATTCTTCAATGAAATCCTTTGGTGAATCTTCTATTTATAAATTGTTTGCCTACTGAAATTGAATTCTTGGGGATTTGCTGCTTTTTGGCATATGGGAGTTATACTTCCCTTTCCTAATATGAATTTTATGAAGGTCAGATTGATTTTACTTCTCCATTTTTAGTTTTTCTAAGAACTCATCTTTCAGTATTTTATACGCAAATCTAAATTTTAGAGTTAAAAAGCACATCGATTTTCATCATTCTGTGTTTGGTTTGGAAAATATATTTTTTAAAGAGTTAGGTTTCAGCCTTGTTCGTCCATAGATGGCTGTGTGCTGGTGGGGGAGTTGCTCACTTTTTCACCCTTTTGTGATAAGGGGCATTTTCTTTGCCTTGCCAGGGTTTGTGCTATCTTATGGGTTTGTTGATGGAGAGAAACAATAGAACTTTTAGAGGATTCGAGAGACCTCTGGAGGAAGTTTAGTCTCTCGTTAGATTTCATGTTTCTCTTTGGGCCTTGGTGGATAATTACAATTATCCTTTAGGTCTTATTTTGTTAGATCAAAGCCCCTTTTTGGCTTAGCTTGGCTCCTCCCACTGTCTTTCTGCATGCCTCTTTTGTATTCTTTCATTTTTATTTCAAGTTCGGTACCTTTAAAAAAAATCATTATGAGAATCCTTATTGGCTCCGAGCACCATTTTACTATCATGTAGGTAAATAATGTTGGTAGAAATATACGAAAGCCAACTGCAGAATTCACGGAGGAAGAATTTTCGTTTCTCATGAAAACCAATGTCGAATCGGTATTCCATTTATCTCAACTGGCTTATCCTCTTCTAAAAGCATCGGGCCGGGGAAGCATCGTGTTCATGTCCTCAGTGTCTAGTTTCGTGTCTCTGAAGTCCATGTCTGTTCAAGGAGCAACCAAAGGTGTGATTGATTTAACCATTATCTTTGGATGCAAACATTATTCTCCTTAGTCTAATATTTCCCAGCAATTCGGGCAGGAGCTATCAATCAACTCACAAAATACTTAGCTTGTGAATGGGCCAAGGACAACATTAGGAGCAATGCGGTCGCCCCGTGGTACATCAAAACGTCAATGGTGGAGCAAGTAATTCTCGCTGTTTTAACCGGTGAAGATTGATTTGTTCATAATGTTTGTCTTGCTGATCTTGTGCTGTATCTTTTCCTTCTAGGTACTGAGTAACAAAGTGTACGTCGAAGAGGTATATTCTCGAACCCCACTTCGACGACTGGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTATTAATGGCTTTGACCCAAGATAACATAAGAAGGAATAACTTTCTTTTATCGGCTCGGCTCGCTAATCAGCTTAGAGGGTCGATTTTCTTGCGCTCCAAGGGATGCAGACCTTTTCGAATCTTATCAATGGAAACTACACATGTTCCCTCTCTTCTCTACCTTTTTATGTTTTCTTTGTATATGGTTCTCAGGACATTTAAAAGGATCTCTGTTGCAGAGTTTATTTGTTCATCTTATAAACGATCATTGCAGATGAGATTCATTTCTTCTTCTTTCAAATTTTTTAAAAAATAACACATATGAGTAAGGAG

mRNA sequence

ATGGCACCACCCCAATCGATGATCGGTGGTCTCTTCGTGGAGCTACGGCCCTCGTCACCGGCGGCACTCGCGGAATCGGAATTTGGAATTGGATTCTTGTTTAGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCAAGAGTTCATACGTGTTCTCGGAATTTGAGTGAGTTGCAACAATGTTTGAGGGATTGGAAGAATTTGGAGTTTGGAGTTTCTGGGTCGGTCTGCGATGTGTCGGTTCGAGCTCAGAGAGAGGAGCTAATGGAAACAGTGGCTGCCAATTTTGATGGGAAGCTCAACATTCTTGTAAATAATGTTGGTAGAAATATACGAAAGCCAACTGCAGAATTCACGGAGGAAGAATTTTCGTTTCTCATGAAAACCAATGTCGAATCGGTATTCCATTTATCTCAACTGGCTTATCCTCTTCTAAAAGCATCGGGCCGGGGAAGCATCGTGTTCATGTCCTCAGTGTCTAGTTTCGTGTCTCTGAAGTCCATGTCTGTTCAAGGAGCAACCAAAGGAGCTATCAATCAACTCACAAAATACTTAGCTTGTGAATGGGCCAAGGACAACATTAGGAGCAATGCGGTCGCCCCGTGGTACATCAAAACGTCAATGGTGGAGCAAGTACTGAGTAACAAAGTGTACGTCGAAGAGGTATATTCTCGAACCCCACTTCGACGACTGGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTATTAATGGCTTTGACCCAAGATAACATAAGAAGGAATAACTTTCTTTTATCGGCTCGGCTCGCTAATCAGCTTAGAGGGTCGATTTTCTTGCGCTCCAAGGGATGCAGACCTTTTCGAATCTTATCAATGGAAACTACACATGTTCCCTCTCTTCTCTACCTTTTTATGTTTTCTTTGTATATGGTTCTCAGGACATTTAAAAGGATCTCTGTTGCAGAGTTTATTTGTTCATCTTATAAACGATCATTGCAGATGAGATTCATTTCTTCTTCTTTCAAATTTTTTAAAAAATAACACATATGAGTAAGGAG

Coding sequence (CDS)

ATGGCACCACCCCAATCGATGATCGGTGGTCTCTTCGTGGAGCTACGGCCCTCGTCACCGGCGGCACTCGCGGAATCGGAATTTGGAATTGGATTCTTGTTTAGGCGTGCGATTGTGGAGGAATTGGTGGGTTTTGGGGCAAGAGTTCATACGTGTTCTCGGAATTTGAGTGAGTTGCAACAATGTTTGAGGGATTGGAAGAATTTGGAGTTTGGAGTTTCTGGGTCGGTCTGCGATGTGTCGGTTCGAGCTCAGAGAGAGGAGCTAATGGAAACAGTGGCTGCCAATTTTGATGGGAAGCTCAACATTCTTGTAAATAATGTTGGTAGAAATATACGAAAGCCAACTGCAGAATTCACGGAGGAAGAATTTTCGTTTCTCATGAAAACCAATGTCGAATCGGTATTCCATTTATCTCAACTGGCTTATCCTCTTCTAAAAGCATCGGGCCGGGGAAGCATCGTGTTCATGTCCTCAGTGTCTAGTTTCGTGTCTCTGAAGTCCATGTCTGTTCAAGGAGCAACCAAAGGAGCTATCAATCAACTCACAAAATACTTAGCTTGTGAATGGGCCAAGGACAACATTAGGAGCAATGCGGTCGCCCCGTGGTACATCAAAACGTCAATGGTGGAGCAAGTACTGAGTAACAAAGTGTACGTCGAAGAGGTATATTCTCGAACCCCACTTCGACGACTGGGAGAACCGAGTGAGGTATCATCTCTGGTTGCATTCCTTTGCCTACCTGCTTCATCTTATATTACTGGCCAAATCATTGGTGTTGATGGAGGGATGTCTATTAATGGCTTTGACCCAAGATAA

Protein sequence

MAPPQSMIGGLFVELRPSSPAALAESEFGIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREELMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKASGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSINGFDPR
Homology
BLAST of MC03g0155 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-97
Identity = 186/244 (76.23%), Postives = 202/244 (82.79%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEEL G GA VHTC+RN  EL+ CL DW    F V+GSVCDVS R+QRE 
Sbjct: 81  GIG----RAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGSVCDVSDRSQREA 140

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETV++ F+GKL+ILVNNVG NIRKP  EFT  EFS LM TN ESVFHL QLAYPLL+ 
Sbjct: 141 LMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRE 200

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           S  GS+VF+SSVS FVSLK+MSVQ +TKGAINQLT+ LACEWAKDNIR NAVAPWYIKTS
Sbjct: 201 SKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTS 260

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK Y+EEVYS TPL RLGEP EVSS VAFLCLPASSYITGQI+ VDGGMSING
Sbjct: 261 MVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSING 320

Query: 269 FDPR 273
           F PR
Sbjct: 321 FFPR 320

BLAST of MC03g0155 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 9.0e-89
Identity = 163/241 (67.63%), Postives = 193/241 (80.08%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RA+VEEL  FGA+VHTCSRN  EL  CL DWK     VSGSVCD SVR QRE+
Sbjct: 22  GIG----RAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGSVCDASVRDQREK 81

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           L++  ++ F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES FHLSQ+A+PLLKA
Sbjct: 82  LIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAFHLSQIAHPLLKA 141

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVF+SSV+  V L S S+ GATKGA+NQLT+ LACEWA DNIR+N VAPWYIKTS
Sbjct: 142 SGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIRTNCVAPWYIKTS 201

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           +VE +L  K +VE V SRTPL R+GEP EVSSLVAFLCLPASSYITGQ+I VDGG ++NG
Sbjct: 202 LVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNG 258

Query: 269 F 270
           F
Sbjct: 262 F 258

BLAST of MC03g0155 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.6e-77
Identity = 146/241 (60.58%), Postives = 187/241 (77.59%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG     A+VEEL   GARVHTC+R+ ++LQ+ LR+W+   F V+ S+CDVS+R QRE+
Sbjct: 20  GIG----EAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTSICDVSLREQREK 79

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETV++ F GKLNILVNNVG  + KPT E+T EEFSFLM TN++S FH+SQLA+PLLKA
Sbjct: 80  LMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAFHISQLAHPLLKA 139

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIV MSS++  V +   S+ GATKGA+NQL + LACEWA DNIR+NA+ PW I T 
Sbjct: 140 SGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIRTNAICPWLITTP 199

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           ++  +LS +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYITGQ+I VDGG+++NG
Sbjct: 200 LISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQVICVDGGLTVNG 256

Query: 269 F 270
           F
Sbjct: 260 F 256

BLAST of MC03g0155 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 4.6e-77
Identity = 148/241 (61.41%), Postives = 186/241 (77.18%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG     A+VEEL   GAR+HTC+R+ ++LQ+ LR W+   F V+ SVCDVS R +RE+
Sbjct: 29  GIG----EAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTSVCDVSSRDKREK 88

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETV+  F+GKLNILVNNVG  I KPT + T E+FSF M TN+ES FHLSQLA+PLLKA
Sbjct: 89  LMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAFHLSQLAHPLLKA 148

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIV +SSVS  V +   S+ G +KGA+NQL + LACEWA DNIR+N+V PW+I+T 
Sbjct: 149 SGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIRTNSVCPWFIETP 208

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           +V + LSN+ + +EV SR P+ R+GE +EVSSLVAFLCLPA+SYITGQ I VDGG ++NG
Sbjct: 209 LVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQTICVDGGFTVNG 265

Query: 269 F 270
           F
Sbjct: 269 F 265

BLAST of MC03g0155 vs. ExPASy Swiss-Prot
Match: Q9ZW20 (Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g29370 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-76
Identity = 146/234 (62.39%), Postives = 182/234 (77.78%), Query Frame = 0

Query: 36  RAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREELMETVAA 95
           +A+VEEL   GARVHTC+R+ ++LQ+ LR+W+     V+ SVCDVS R QRE+LMETV++
Sbjct: 32  KAVVEELAMLGARVHTCARDETQLQESLREWQAKGLQVTTSVCDVSSRDQREKLMETVSS 91

Query: 96  NFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKASGRGSIV 155
            F GKL+ILV NVG  + KPT E T EEFSF++ TN+ES FH SQLA+PLLKASG G+IV
Sbjct: 92  LFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLESTFHFSQLAHPLLKASGSGNIV 151

Query: 156 FMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVLS 215
            MSSV+  V+L + S+ GATKGA+NQL + LACEWA DNIR+N+V PW+I T   +  L 
Sbjct: 152 LMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWASDNIRANSVCPWFITTPSTKDFLG 211

Query: 216 NKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSINGF 270
           +K   E+V S TPLRR+GE +EVSSLVAFLCLPA+SYITGQ I VDGG +INGF
Sbjct: 212 DKDVKEKVESVTPLRRVGEANEVSSLVAFLCLPAASYITGQTICVDGGFTINGF 265

BLAST of MC03g0155 vs. NCBI nr
Match: XP_022141872.1 (tropinone reductase homolog At5g06060-like [Momordica charantia])

HSP 1 Score: 468 bits (1203), Expect = 5.86e-164
Identity = 240/244 (98.36%), Postives = 240/244 (98.36%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE
Sbjct: 93  GIG----RAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 152

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA
Sbjct: 153 LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 212

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS
Sbjct: 213 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 272

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING
Sbjct: 273 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 332

Query: 269 FDPR 272
           FDPR
Sbjct: 333 FDPR 332

BLAST of MC03g0155 vs. NCBI nr
Match: XP_022998606.1 (tropinone reductase homolog At5g06060-like [Cucurbita maxima])

HSP 1 Score: 437 bits (1125), Expect = 4.69e-152
Identity = 223/243 (91.77%), Postives = 231/243 (95.06%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN SEL+QCLRDWK+LEF VSGSVCDVSVRAQREE
Sbjct: 95  GIG----RAIVEELVGFGARVHTCSRNESELKQCLRDWKDLEFEVSGSVCDVSVRAQREE 154

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LM+TVA  F+GKLNILVNNVGRNIRKPT EFT+EEFSFLMKTN+ESVFHLSQLA+P LKA
Sbjct: 155 LMKTVADTFNGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNIESVFHLSQLAHPFLKA 214

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS
Sbjct: 215 SGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 274

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 275 MVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 333

Query: 269 FDP 271
           FDP
Sbjct: 335 FDP 333

BLAST of MC03g0155 vs. NCBI nr
Match: XP_023523143.1 (tropinone reductase homolog At5g06060-like [Cucurbita pepo subsp. pepo] >XP_023523926.1 tropinone reductase homolog At5g06060-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 437 bits (1123), Expect = 9.45e-152
Identity = 223/243 (91.77%), Postives = 230/243 (94.65%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN +EL+QCLRDWK+LEF VSGSVCDVSVRAQREE
Sbjct: 95  GIG----RAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDVSVRAQREE 154

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LM+TVA  FDGKLNILVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLA+P LKA
Sbjct: 155 LMKTVADTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKA 214

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWYIKTS
Sbjct: 215 SGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTS 274

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 275 MVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 333

Query: 269 FDP 271
           FDP
Sbjct: 335 FDP 333

BLAST of MC03g0155 vs. NCBI nr
Match: KAG6607269.1 (Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436 bits (1122), Expect = 1.34e-151
Identity = 223/243 (91.77%), Postives = 230/243 (94.65%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN +EL+QCLRDWK+LEF VSGSVCDVSVRAQREE
Sbjct: 95  GIG----RAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDVSVRAQREE 154

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LM+TVA  FDGKLNILVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLA+P LKA
Sbjct: 155 LMKTVADTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKA 214

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWYIKTS
Sbjct: 215 SGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTS 274

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 275 MVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 333

Query: 269 FDP 271
           FDP
Sbjct: 335 FDP 333

BLAST of MC03g0155 vs. NCBI nr
Match: XP_022948630.1 (tropinone reductase homolog At5g06060-like [Cucurbita moschata])

HSP 1 Score: 436 bits (1122), Expect = 1.34e-151
Identity = 223/243 (91.77%), Postives = 230/243 (94.65%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN +EL+QCLRDWK+LEF VSGSVCDVSVRAQREE
Sbjct: 95  GIG----RAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDVSVRAQREE 154

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LM+TVA  FDGKLNILVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLA+P LKA
Sbjct: 155 LMKTVADTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKA 214

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWYIKTS
Sbjct: 215 SGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTS 274

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 275 MVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 333

Query: 269 FDP 271
           FDP
Sbjct: 335 FDP 333

BLAST of MC03g0155 vs. ExPASy TrEMBL
Match: A0A6J1CL23 (tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC111012129 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 2.84e-164
Identity = 240/244 (98.36%), Postives = 240/244 (98.36%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE
Sbjct: 93  GIG----RAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 152

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA
Sbjct: 153 LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 212

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS
Sbjct: 213 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 272

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING
Sbjct: 273 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 332

Query: 269 FDPR 272
           FDPR
Sbjct: 333 FDPR 332

BLAST of MC03g0155 vs. ExPASy TrEMBL
Match: A0A6J1KAL5 (tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111493193 PE=4 SV=1)

HSP 1 Score: 437 bits (1125), Expect = 2.27e-152
Identity = 223/243 (91.77%), Postives = 231/243 (95.06%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN SEL+QCLRDWK+LEF VSGSVCDVSVRAQREE
Sbjct: 95  GIG----RAIVEELVGFGARVHTCSRNESELKQCLRDWKDLEFEVSGSVCDVSVRAQREE 154

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LM+TVA  F+GKLNILVNNVGRNIRKPT EFT+EEFSFLMKTN+ESVFHLSQLA+P LKA
Sbjct: 155 LMKTVADTFNGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNIESVFHLSQLAHPFLKA 214

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS
Sbjct: 215 SGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 274

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 275 MVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 333

Query: 269 FDP 271
           FDP
Sbjct: 335 FDP 333

BLAST of MC03g0155 vs. ExPASy TrEMBL
Match: A0A6J1GAE7 (tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC111452252 PE=4 SV=1)

HSP 1 Score: 436 bits (1122), Expect = 6.49e-152
Identity = 223/243 (91.77%), Postives = 230/243 (94.65%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN +EL+QCLRDWK+LEF VSGSVCDVSVRAQREE
Sbjct: 95  GIG----RAIVEELVGFGARVHTCSRNENELKQCLRDWKDLEFEVSGSVCDVSVRAQREE 154

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LM+TVA  FDGKLNILVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLA+P LKA
Sbjct: 155 LMKTVADTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAHPFLKA 214

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWA DNIRSNAVAPWYIKTS
Sbjct: 215 SGAGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWANDNIRSNAVAPWYIKTS 274

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 275 MVEQVLSNKAYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 333

Query: 269 FDP 271
           FDP
Sbjct: 335 FDP 333

BLAST of MC03g0155 vs. ExPASy TrEMBL
Match: A0A5D3BGS1 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001920 PE=4 SV=1)

HSP 1 Score: 436 bits (1120), Expect = 1.22e-151
Identity = 222/244 (90.98%), Postives = 228/244 (93.44%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN  EL+QCLR WK+LEF VSGSVCDVSVRAQREE
Sbjct: 93  GIG----RAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVSGSVCDVSVRAQREE 152

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LME     FDGKLNILVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLAYPLLK+
Sbjct: 153 LMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKS 212

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS
Sbjct: 213 SGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 272

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 273 MVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 332

Query: 269 FDPR 272
           FDP+
Sbjct: 333 FDPK 332

BLAST of MC03g0155 vs. ExPASy TrEMBL
Match: A0A1S3C5I5 (tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE=4 SV=1)

HSP 1 Score: 436 bits (1120), Expect = 1.22e-151
Identity = 222/244 (90.98%), Postives = 228/244 (93.44%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEELVGFGARVHTCSRN  EL+QCLR WK+LEF VSGSVCDVSVRAQREE
Sbjct: 93  GIG----RAIVEELVGFGARVHTCSRNEGELRQCLRHWKDLEFEVSGSVCDVSVRAQREE 152

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LME     FDGKLNILVNNVGRNIRKPT EFT+EEFSFLMKTNVESVFHLSQLAYPLLK+
Sbjct: 153 LMENAGNTFDGKLNILVNNVGRNIRKPTTEFTDEEFSFLMKTNVESVFHLSQLAYPLLKS 212

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS
Sbjct: 213 SGEGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 272

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK YVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMS+NG
Sbjct: 273 MVEQVLSNKTYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSVNG 332

Query: 269 FDPR 272
           FDP+
Sbjct: 333 FDPK 332

BLAST of MC03g0155 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 1.7e-98
Identity = 186/244 (76.23%), Postives = 202/244 (82.79%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RAIVEEL G GA VHTC+RN  EL+ CL DW    F V+GSVCDVS R+QRE 
Sbjct: 81  GIG----RAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGSVCDVSDRSQREA 140

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETV++ F+GKL+ILVNNVG NIRKP  EFT  EFS LM TN ESVFHL QLAYPLL+ 
Sbjct: 141 LMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVFHLCQLAYPLLRE 200

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           S  GS+VF+SSVS FVSLK+MSVQ +TKGAINQLT+ LACEWAKDNIR NAVAPWYIKTS
Sbjct: 201 SKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIRINAVAPWYIKTS 260

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           MVEQVLSNK Y+EEVYS TPL RLGEP EVSS VAFLCLPASSYITGQI+ VDGGMSING
Sbjct: 261 MVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQILCVDGGMSING 320

Query: 269 FDPR 273
           F PR
Sbjct: 321 FFPR 320

BLAST of MC03g0155 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 328.2 bits (840), Expect = 6.4e-90
Identity = 163/241 (67.63%), Postives = 193/241 (80.08%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG    RA+VEEL  FGA+VHTCSRN  EL  CL DWK     VSGSVCD SVR QRE+
Sbjct: 22  GIG----RAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGSVCDASVRDQREK 81

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           L++  ++ F GKLNIL+NNVG N+RKPT E++ EE++ +M TN+ES FHLSQ+A+PLLKA
Sbjct: 82  LIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAFHLSQIAHPLLKA 141

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIVF+SSV+  V L S S+ GATKGA+NQLT+ LACEWA DNIR+N VAPWYIKTS
Sbjct: 142 SGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIRTNCVAPWYIKTS 201

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           +VE +L  K +VE V SRTPL R+GEP EVSSLVAFLCLPASSYITGQ+I VDGG ++NG
Sbjct: 202 LVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQVISVDGGFTVNG 258

Query: 269 F 270
           F
Sbjct: 262 F 258

BLAST of MC03g0155 vs. TAIR 10
Match: AT2G29290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 3.0e-79
Identity = 144/235 (61.28%), Postives = 185/235 (78.72%), Query Frame = 0

Query: 35  RRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREELMETVA 94
           R A+VEEL   GARVHTC+R+ ++LQ+ LR+W+   F V+ S+CDVS+R QRE+LMETV+
Sbjct: 5   REAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTSICDVSLREQREKLMETVS 64

Query: 95  ANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKASGRGSI 154
           + F GKLNILVNNVG  + KPT E+T EEFSFLM TN++S FH+SQLA+PLLKASG GSI
Sbjct: 65  SLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAFHISQLAHPLLKASGSGSI 124

Query: 155 VFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTSMVEQVL 214
           V MSS++  V +   S+ GATKGA+NQL + LACEWA DNIR+NA+ PW I T ++  +L
Sbjct: 125 VLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIRTNAICPWLITTPLISDLL 184

Query: 215 SNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSINGF 270
           S +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYITGQ+I VDGG+++NGF
Sbjct: 185 SVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQVICVDGGLTVNGF 239

BLAST of MC03g0155 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.1e-78
Identity = 146/241 (60.58%), Postives = 187/241 (77.59%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG     A+VEEL   GARVHTC+R+ ++LQ+ LR+W+   F V+ S+CDVS+R QRE+
Sbjct: 20  GIG----EAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTSICDVSLREQREK 79

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETV++ F GKLNILVNNVG  + KPT E+T EEFSFLM TN++S FH+SQLA+PLLKA
Sbjct: 80  LMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAFHISQLAHPLLKA 139

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIV MSS++  V +   S+ GATKGA+NQL + LACEWA DNIR+NA+ PW I T 
Sbjct: 140 SGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIRTNAICPWLITTP 199

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           ++  +LS +   +E   RTP+ R+GE +EVS LVAFLCLPA+SYITGQ+I VDGG+++NG
Sbjct: 200 LISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQVICVDGGLTVNG 256

Query: 269 F 270
           F
Sbjct: 260 F 256

BLAST of MC03g0155 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 3.3e-78
Identity = 148/241 (61.41%), Postives = 186/241 (77.18%), Query Frame = 0

Query: 29  GIGFLFRRAIVEELVGFGARVHTCSRNLSELQQCLRDWKNLEFGVSGSVCDVSVRAQREE 88
           GIG     A+VEEL   GAR+HTC+R+ ++LQ+ LR W+   F V+ SVCDVS R +RE+
Sbjct: 29  GIG----EAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTSVCDVSSRDKREK 88

Query: 89  LMETVAANFDGKLNILVNNVGRNIRKPTAEFTEEEFSFLMKTNVESVFHLSQLAYPLLKA 148
           LMETV+  F+GKLNILVNNVG  I KPT + T E+FSF M TN+ES FHLSQLA+PLLKA
Sbjct: 89  LMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAFHLSQLAHPLLKA 148

Query: 149 SGRGSIVFMSSVSSFVSLKSMSVQGATKGAINQLTKYLACEWAKDNIRSNAVAPWYIKTS 208
           SG GSIV +SSVS  V +   S+ G +KGA+NQL + LACEWA DNIR+N+V PW+I+T 
Sbjct: 149 SGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIRTNSVCPWFIETP 208

Query: 209 MVEQVLSNKVYVEEVYSRTPLRRLGEPSEVSSLVAFLCLPASSYITGQIIGVDGGMSING 268
           +V + LSN+ + +EV SR P+ R+GE +EVSSLVAFLCLPA+SYITGQ I VDGG ++NG
Sbjct: 209 LVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQTICVDGGFTVNG 265

Query: 269 F 270
           F
Sbjct: 269 F 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZW122.4e-9776.23Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9LHT09.0e-8967.63Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW131.6e-7760.58Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Q9ZW194.6e-7761.41Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=... [more]
Q9ZW201.8e-7662.39Tropinone reductase homolog At2g29370 OS=Arabidopsis thaliana OX=3702 GN=At2g293... [more]
Match NameE-valueIdentityDescription
XP_022141872.15.86e-16498.36tropinone reductase homolog At5g06060-like [Momordica charantia][more]
XP_022998606.14.69e-15291.77tropinone reductase homolog At5g06060-like [Cucurbita maxima][more]
XP_023523143.19.45e-15291.77tropinone reductase homolog At5g06060-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6607269.11.34e-15191.77Tropinone reductase-like, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022948630.11.34e-15191.77tropinone reductase homolog At5g06060-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CL232.84e-16498.36tropinone reductase homolog At5g06060-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1KAL52.27e-15291.77tropinone reductase homolog At5g06060-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GAE76.49e-15291.77tropinone reductase homolog At5g06060-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5D3BGS11.22e-15190.98Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C5I51.22e-15190.98tropinone reductase homolog At5g06060 OS=Cucumis melo OX=3656 GN=LOC103497133 PE... [more]
Match NameE-valueIdentityDescription
AT2G29260.11.7e-9876.23NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G06060.16.4e-9067.63NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.13.0e-7961.28NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29290.21.1e-7860.58NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.13.3e-7861.41NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 152..160
score: 48.0
coord: 99..110
score: 43.41
coord: 172..191
score: 31.56
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 146..162
score: 32.1
coord: 172..191
score: 36.93
coord: 99..110
score: 50.8
coord: 193..210
score: 39.59
coord: 228..248
score: 34.57
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 36..265
e-value: 3.2E-55
score: 187.2
NoneNo IPR availableGENE3D3.40.50.720coord: 26..268
e-value: 7.2E-69
score: 233.8
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 77..269
e-value: 7.6E-21
score: 72.2
NoneNo IPR availablePANTHERPTHR42898:SF2TROPINONE REDUCTASE-LIKE PROTEIN, CHLOROPLASTICcoord: 36..272
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 36..272
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 36..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC03g0155.1MC03g0155.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity