MC03g0090 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0090
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase
Location	MC03: 1345014 .. 1346961 (+)
RNA-Seq Expression	MC03g0090
Synteny	MC03g0090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCTGAAACAATGAGAAGCGGCGGCAATGGCGAACTCGTCGCCGTAATTTTCGGAGTGACAGGGTTGGTCGGAAAAGAGCTGGCGATAGCCCTTCTGGCAACCGCTGGCTGGAAAGTCTACGGCGTGGCGCGGCGGCCAGACAACGTCAGCCCCATCGCCGATCCCAACTTCCACTTCATCTCCTGCGATTTGCTCGACCGTCGGTCAACTTACCGGAATCTCTCGCCGCTCCGCCACGTCACTCACGTCTTCTGGATCACCTGGGCCGCCCAGCTCCGCTTCGACTCCCGCGAGTGCTCCGACCAGAACAAGGCCATGTTGGCCCACGCGCTCGACGCGCTCCTCCCGGCGGCGCCCTCCCTCCGCCACGTGTCTCTCCAGACCGGCGTCAAGCACTACGTTTCCCTCACCGGCGGCGGCGGTGGCGGAGGGGAATCTGCCGTTTTCTGCGAGGACAGTCCGAGATCGGAGTCCGGGAACAATTTCTACTACGCGTTGGAGGATCTCCTCCGTGAGCGGCTGGGCCGGAGGGTGGCGTGGACCGTTCTCCGGCCGGGTCTGATTATCGGGTCATCGAACCGGAGTTTCTTCAATTTCATGGGGAGTTTGAGTGTTTATGGCGCGATTTGTAAGAAATTGAAGCTGCCGTTCGTGTTCGGCGGCACCGCGGAGTGTTGGGCGGAGGTTTATATTGATGGATCCGACGCTCGGCTGGTTGCCCACCAGCACATATGGGCCGCCACCAAACATCTCCCGCTAAATGGTATGTCATCACTCGAAAGTTTAAATTCTTGACCTCTATTAATATGTAGTCTTATTTAAAGAAGAAGAAAATTTTAATACTCCCCCACTATTTATTCTTTTCTTTTTCTACACTAGAAAATTTTTTGGTATGACCATCGATAAAAAGTCTACCGAAAATTAGGTAGTGGCAAAAATGTCTTGTGATATCATCTAATGTGTTCGAGTCCTACGTTGCAGCCACACGACATTGATTCTAGGAGATAAAAAAATTATCGTTAAAAAGCTGATAATTCAATACTTAAAAATAAAAAAAATGAAAACCTATTTCTTTTCTCACTTATTTAAAAAAAGAAATTTAATGTGGTATGCAGGTAATCTTCAATCTACACATAAATACTAATTTTTCATACTTGACTTTACATGCTAGTAAGACTATTTACTGATTTTTATGATGATTTTTATGATGTAGCGTATGTTTATGCATCATACATAAGCGAAATAAAAAAGTTAGTATAGTATATAGACAAGTATTGTTTTAATTTTCTTTATAATAATCTATTAGAATAAACTACACCTAACATTAACCGTAAACGATATTATAAACCATAGTTAAAATCAACTATAATATGTAGCAGAGGCTACACTAAAATAGACCATTGTTTACTATACCACCATATAATTAGAAAATTTTCCACACCATTAAGCTATGTTTAGCTTGAGTACATTTCAAAATTCAAGCATTAGAAAGAGTAAAAATCGTCTCATTTACAATTCGGATATTGAATATAAAACACTTATTAATCTTCTGTCGACATGTACATGAATAGGCACGGGCGAGGGGGAAGCTTTCAACGTGTGCAATGGGTGGAGCTTCAAGTGGAAGGAGATATGGGGCGCGGTGGGGGAGAAGTTCGGGGCGGCGACAGAGGACGTGAGGTTTTCGGCGGAGTTCCGTTACACGGCGGCAATGGCGGACAAAGGGAAGTTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTAGTTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGATTCTTGGACAGCTTGTTTAGGTTGCCGGAAAAAATGGTGGCCAGTCGACAAAAGGCTGACCAATTTGGGTTTACTATAAAACACAACATGTTGGATTCCATGTTCCATTGGATTGATGTCATGAGACATGACAAATTGATTCCT

mRNA sequence

ATGGCGGCTGAAACAATGAGAAGCGGCGGCAATGGCGAACTCGTCGCCGTAATTTTCGGAGTGACAGGGTTGGTCGGAAAAGAGCTGGCGATAGCCCTTCTGGCAACCGCTGGCTGGAAAGTCTACGGCGTGGCGCGGCGGCCAGACAACGTCAGCCCCATCGCCGATCCCAACTTCCACTTCATCTCCTGCGATTTGCTCGACCGTCGGTCAACTTACCGGAATCTCTCGCCGCTCCGCCACGTCACTCACGTCTTCTGGATCACCTGGGCCGCCCAGCTCCGCTTCGACTCCCGCGAGTGCTCCGACCAGAACAAGGCCATGTTGGCCCACGCGCTCGACGCGCTCCTCCCGGCGGCGCCCTCCCTCCGCCACGTGTCTCTCCAGACCGGCGTCAAGCACTACGTTTCCCTCACCGGCGGCGGCGGTGGCGGAGGGGAATCTGCCGTTTTCTGCGAGGACAGTCCGAGATCGGAGTCCGGGAACAATTTCTACTACGCGTTGGAGGATCTCCTCCGTGAGCGGCTGGGCCGGAGGGTGGCGTGGACCGTTCTCCGGCCGGGTCTGATTATCGGGTCATCGAACCGGAGTTTCTTCAATTTCATGGGGAGTTTGAGTGTTTATGGCGCGATTTGTAAGAAATTGAAGCTGCCGTTCGTGTTCGGCGGCACCGCGGAGTGTTGGGCGGAGGTTTATATTGATGGATCCGACGCTCGGCTGGTTGCCCACCAGCACATATGGGCCGCCACCAAACATCTCCCGCTAAATGGCACGGGCGAGGGGGAAGCTTTCAACGTGTGCAATGGGTGGAGCTTCAAGTGGAAGGAGATATGGGGCGCGGTGGGGGAGAAGTTCGGGGCGGCGACAGAGGACGTGAGGTTTTCGGCGGAGTTCCGTTACACGGCGGCAATGGCGGACAAAGGGAAGTTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTAGTTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGATTCTTGGACAGCTTGTTTAGGTTGCCGGAAAAAATGGTGGCCAGTCGACAAAAGGCTGACCAATTTGGGTTTACTATAAAACACAACATGTTGGATTCCATGTTCCATTGGATTGATGTCATGAGACATGACAAATTGATTCCT

Coding sequence (CDS)

ATGGCGGCTGAAACAATGAGAAGCGGCGGCAATGGCGAACTCGTCGCCGTAATTTTCGGAGTGACAGGGTTGGTCGGAAAAGAGCTGGCGATAGCCCTTCTGGCAACCGCTGGCTGGAAAGTCTACGGCGTGGCGCGGCGGCCAGACAACGTCAGCCCCATCGCCGATCCCAACTTCCACTTCATCTCCTGCGATTTGCTCGACCGTCGGTCAACTTACCGGAATCTCTCGCCGCTCCGCCACGTCACTCACGTCTTCTGGATCACCTGGGCCGCCCAGCTCCGCTTCGACTCCCGCGAGTGCTCCGACCAGAACAAGGCCATGTTGGCCCACGCGCTCGACGCGCTCCTCCCGGCGGCGCCCTCCCTCCGCCACGTGTCTCTCCAGACCGGCGTCAAGCACTACGTTTCCCTCACCGGCGGCGGCGGTGGCGGAGGGGAATCTGCCGTTTTCTGCGAGGACAGTCCGAGATCGGAGTCCGGGAACAATTTCTACTACGCGTTGGAGGATCTCCTCCGTGAGCGGCTGGGCCGGAGGGTGGCGTGGACCGTTCTCCGGCCGGGTCTGATTATCGGGTCATCGAACCGGAGTTTCTTCAATTTCATGGGGAGTTTGAGTGTTTATGGCGCGATTTGTAAGAAATTGAAGCTGCCGTTCGTGTTCGGCGGCACCGCGGAGTGTTGGGCGGAGGTTTATATTGATGGATCCGACGCTCGGCTGGTTGCCCACCAGCACATATGGGCCGCCACCAAACATCTCCCGCTAAATGGCACGGGCGAGGGGGAAGCTTTCAACGTGTGCAATGGGTGGAGCTTCAAGTGGAAGGAGATATGGGGCGCGGTGGGGGAGAAGTTCGGGGCGGCGACAGAGGACGTGAGGTTTTCGGCGGAGTTCCGTTACACGGCGGCAATGGCGGACAAAGGGAAGTTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTAGTTCCGACGGAAATGGAGGAGCTGGCCAATTGGGGATTCTTGGACAGCTTGTTTAGGTTGCCGGAAAAAATGGTGGCCAGTCGACAAAAGGCTGACCAATTTGGGTTTACTATAAAACACAACATGTTGGATTCCATGTTCCATTGGATTGATGTCATGAGACATGACAAATTGATTCCT

Protein sequence

MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRVAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLDSMFHWIDVMRHDKLIP

Homology

[Close]

BLAST of MC03g0090 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 4.8e-64
Identity = 154/376 (40.96%), Postives = 203/376 (53.99%), Query Frame = 0


Query: 15  VAVIFGVTGLVGKELA--IALLATAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRR 74
           VA+I GVTG+VG  LA  + +  T G  WKVYGVARRP   +  AD    +I CD+ D  
Sbjct: 28  VALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISDAS 87

Query: 75  STYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHVSLQT 134
            T+  LSPL  VTH+FW+TWA   R    EC + N  ML + L+AL+P A +L H+ LQT
Sbjct: 88  DTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQT 147

Query: 135 GVKHYVS-LTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRVA--WTVLRP 194
           G KHY+      G        F ED PR  +  NFYY LED+L E   ++    W+V RP
Sbjct: 148 GHKHYIGPFEAFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVEASEKKAGLNWSVHRP 207

Query: 195 GLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQHIW 254
            +I G S  S  N +G+L VY AICK    P  F GT   W   Y   SDA L+A   IW
Sbjct: 208 AVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAW-NCYSVASDADLIAEHQIW 267

Query: 255 AATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTA---AM 314
           AA     ++   + EAFN  NG  FKWK +W  + E+FG   E   F    + T+    M
Sbjct: 268 AA-----VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFG--VEYAEFDESEKPTSLVERM 327

Query: 315 ADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLD 374
            DKG +W EIV E GL  T++E +A W F D +    E ++ S  K+ + G+    N  +
Sbjct: 328 KDKGPVWEEIVRENGLHTTKLEGVATWWFADVILG-GECLLDSMNKSKEHGYLGFRNTKN 387

Query: 375 SMFHWIDVMRHDKLIP 381
           S+   ID M+  K++P
Sbjct: 388 SLISVIDKMKAHKIVP 390

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BLAST of MC03g0090 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 6.3e-64
Identity = 148/374 (39.57%), Postives = 203/374 (54.28%), Query Frame = 0


Query: 13  ELVAVIFGVTGLVGKELA--IALLATAG--WKVYGVARRPDNVSPIADPNFHFISCDLLD 72
           E VA+I GVTG+VG  LA  + L  T G  WKVYGVARRP   +  AD    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 73  RRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHVSL 132
              T   LSPL  VTHVF++TW  +   +S  C + N +ML + L A++P AP+LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 133 QTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGR--RVAWTVLR 192
           QTG KHY+       G      F ED PR +   NFYY  ED+L E + +   V W++ R
Sbjct: 146 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIKKIETVTWSIHR 205

Query: 193 PGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQHI 252
           P +I G S  S  N +G+L VY AICK    P +F G+ + W E ++  SDA L+A Q I
Sbjct: 206 PNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIAEQQI 265

Query: 253 WAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAMAD 312
           WAA     ++   + EAFN  N   FKWK +W  + E+FG                 M  
Sbjct: 266 WAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKG 325

Query: 313 KGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLDSM 372
           K ++W E+V+E  L   ++EE+  W F D +  + E M+ S  K+ ++GF    N  +S 
Sbjct: 326 KERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNSNNSF 385

Query: 373 FHWIDVMRHDKLIP 381
             WID  +  K++P
Sbjct: 386 ISWIDKYKAFKIVP 388

[Close]

BLAST of MC03g0090 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.2e-62
Identity = 148/376 (39.36%), Postives = 201/376 (53.46%), Query Frame = 0


Query: 11  NGELVAVIFGVTGLVGKELA--IALLATAG--WKVYGVARRPDNVSPIADPNFHFISCDL 70
           N E VA+I GVTG+VG  LA  + L  T G  WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 71  LDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHV 130
           LD       LSPL  VTH+F+ TW    R   +E  + N  ML + L+A++P  P+L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 131 SLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRR--VAWTV 190
            LQTG KHY+                ED PR +S  N+YY  ED+L E + ++  + W+V
Sbjct: 144 CLQTGRKHYLGAFEDLKSKCHDPPLTEDLPRLDS-QNYYYTQEDILFEEVQKKEGLTWSV 203

Query: 191 LRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQ 250
            RPG I G S  S  N +G+L VY AICK       F G    W + Y D SDA L+A  
Sbjct: 204 HRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAW-DGYSDCSDADLIAEH 263

Query: 251 HIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAM 310
            IWAA     ++   + EAFNV NG  FKWK  W  + E+FG    +     + +    M
Sbjct: 264 QIWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEEGQQVKLQDLM 323

Query: 311 ADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLD 370
            DKG +W +IV E GL  T++E++ NW F D +    E  + +  K+ + GF    N  +
Sbjct: 324 KDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLGFRNSKN 383

Query: 371 SMFHWIDVMRHDKLIP 381
           S   WID ++  K++P
Sbjct: 384 SFISWIDKVKAFKIVP 388

[Close]

BLAST of MC03g0090 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.2e-62
Identity = 148/376 (39.36%), Postives = 198/376 (52.66%), Query Frame = 0


Query: 11  NGELVAVIFGVTGLVGKELAIAL----LATAGWKVYGVARRPDNVSPIADPNFHFISCDL 70
           N E VA+I GVTG+VG  LA  L         WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 71  LDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHV 130
           LD       LSPL  VTH+F+ TW    R   +E  + N  ML + L+A++P  P+L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 131 SLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRR--VAWTV 190
            LQTG KHY+                ED PR +S  NFYY  ED+L E + ++  + W+V
Sbjct: 144 CLQTGRKHYLGAFEDWKIKRHDPPLTEDLPRLDS-QNFYYTQEDILFEEVQKKESLTWSV 203

Query: 191 LRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQ 250
            RPG I G S  S  N +G+L VY AICK       F G    W + Y D SDA L+A  
Sbjct: 204 HRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAW-DGYSDCSDADLIAEH 263

Query: 251 HIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAM 310
            IWAA     ++   + EAFNV NG  FKWK  W  + E+FG    +     E +    M
Sbjct: 264 QIWAA-----VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEEGKEVKLQDLM 323

Query: 311 ADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLD 370
            DKG +W +IV E GL  T++E++ NW F D +    E  + +  K+ + GF    N  +
Sbjct: 324 KDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLGFRNSKN 383

Query: 371 SMFHWIDVMRHDKLIP 381
           S   WID ++  K++P
Sbjct: 384 SFISWIDKVKAFKIVP 388

[Close]

BLAST of MC03g0090 vs. ExPASy Swiss-Prot
Match: A0A221J5X6 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta racemosa OX=54731 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.7e-61
Identity = 147/375 (39.20%), Postives = 202/375 (53.87%), Query Frame = 0


Query: 13  ELVAVIFGVTGLVGKELA--IALLATAG--WKVYGVARRPDNVSPIADPNFHFISCDLLD 72
           + VA+I GVTGLVG  LA  + L  T G  WKVYGVARR    S   D    +ISCD+ +
Sbjct: 29  QCVALIVGVTGLVGNSLAEMLPLPDTPGGPWKVYGVARRA-RPSWNEDQPMTYISCDVSN 88

Query: 73  RRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHVSL 132
                  LSPL  VTH+F+ TW +  R    E  + N  ML + LDA++P  P+L+H+ L
Sbjct: 89  TGEVEAKLSPLSDVTHIFYATWTS--RSTEEENCEANGKMLKNVLDAMIPNCPNLKHICL 148

Query: 133 QTGVKHYV-SLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRR--VAWTVL 192
           QTG  HYV S+      G       ED PR ++ NNFYY  ED+L E + R+  + W+V 
Sbjct: 149 QTGRFHYVASVVDWKINGSHDTPLTEDLPRLKT-NNFYYTQEDILLEEVKRKEGLTWSVH 208

Query: 193 RPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQH 252
           RPG I G S  S  N +G+L VY AICK+      F G    W + + D +DA L+A Q 
Sbjct: 209 RPGTIFGFSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAW-DGHSDCADADLIAEQQ 268

Query: 253 IWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAMA 312
           IWAA     L+   + +AFNV NG  FKWK +W  + ++FG    D     + R    M 
Sbjct: 269 IWAA-----LDPHAKNQAFNVSNGDLFKWKHLWKVLADQFGVECGDYEEGQQLRLQDVMK 328

Query: 313 DKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLDS 372
           DKG +W +IV E GL  T++E++  W F D++    E  + S  K+ + GF    N  + 
Sbjct: 329 DKGPVWDKIVAENGLSNTKLEDVGKWWFSDTIL-WNECRLDSMNKSKEHGFLGFRNSKNC 388

Query: 373 MFHWIDVMRHDKLIP 381
             +WI  ++   L+P
Sbjct: 389 FLYWIHKVKAYNLVP 392

[Close]

BLAST of MC03g0090 vs. NCBI nr
Match: XP_022150915.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Momordica charantia])

HSP 1 Score: 786 bits (2029), Expect = 6.78e-287
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 1   MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFH 60
           MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFH
Sbjct: 15  MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFH 74

Query: 61  FISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAA 120
           FISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAA
Sbjct: 75  FISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAA 134

Query: 121 PSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRV 180
           PSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRV
Sbjct: 135 PSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRV 194

Query: 181 AWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARL 240
           AWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARL
Sbjct: 195 AWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARL 254

Query: 241 VAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRY 300
           VAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRY
Sbjct: 255 VAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRY 314

Query: 301 TAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKH 360
           TAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKH
Sbjct: 315 TAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKH 374

Query: 361 NMLDSMFHWIDVMRHDKLIP 380
           NMLDSMFHWIDVMRHDKLIP
Sbjct: 375 NMLDSMFHWIDVMRHDKLIP 394

[Close]

BLAST of MC03g0090 vs. NCBI nr
Match: XP_038895390.1 ((S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida])

HSP 1 Score: 626 bits (1615), Expect = 7.31e-224
Identity = 307/393 (78.12%), Postives = 342/393 (87.02%), Query Frame = 0


Query: 1   MAAETMRSG--GNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MAAE + +    N ELVAVIFGVTGLVGKELA  LL+TAGWKVYGVARRPDN+SPI+ PN
Sbjct: 1   MAAEIINTNLVANAELVAVIFGVTGLVGKELAKTLLSTAGWKVYGVARRPDNISPISHPN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           FHFISCDLLDRRS + NLSP+RHVTH+FWITWAAQLRFDS+ CSDQN+AMLA+ALDA+LP
Sbjct: 61  FHFISCDLLDRRSAHSNLSPVRHVTHLFWITWAAQLRFDSQGCSDQNRAMLANALDAILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLT---GGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRER 180
           +AP+LRHVSLQTG+KHY SLT    G GGGGE A +CEDSPR+ESGNNFYY LEDLLRER
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEVA-YCEDSPRAESGNNFYYVLEDLLRER 180

Query: 181 L--GRRVA-WTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVY 240
           L  GRRV  W+VLRPGLI+GSSNR+FFNFMGSL VYGAICKKLK+PFVFGGTA CW EVY
Sbjct: 181 LSGGRRVVGWSVLRPGLILGSSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVY 240

Query: 241 IDGSDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAAT--- 300
           IDGSDARL A QH+WAATK + +N T +GEAFNVCNGWSF+WKEIWGAV EK G A    
Sbjct: 241 IDGSDARLTAEQHVWAATKGIKINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVAAAEK 300

Query: 301 --EDVRFSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVAS 360
             E + FSAEF YTAAM DKGKLWAEIVEEEGLVPTEMEELANWGFLD+LFRLPEKMVAS
Sbjct: 301 EDERIMFSAEFHYTAAMGDKGKLWAEIVEEEGLVPTEMEELANWGFLDTLFRLPEKMVAS 360

Query: 361 RQKADQFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           R K+D+FGFT+K+ MLDS+ +WIDVMR+DKLIP
Sbjct: 361 RAKSDRFGFTVKYKMLDSILYWIDVMRNDKLIP 392

[Close]

BLAST of MC03g0090 vs. NCBI nr
Match: KAG7019139.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 620 bits (1600), Expect = 1.26e-221
Identity = 305/388 (78.61%), Postives = 336/388 (86.60%), Query Frame = 0


Query: 1   MAAETMRSG--GNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MA E+M +    NGELVAVIFGVTGLVGKELA  LL+TAGWKVYGVARRPDN+SPI+DPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKLLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           FHFISCDLLDRRS + NLSPL HVTH+FWITWAAQLRFDS++CSDQNKAMLA+ALDA+LP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLCHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERL-G 180
           +AP+LRHVSLQTGVKHY SLTG  GGGG   V+ EDSPR+ESGNNFYY LEDLLRERL G
Sbjct: 121 SAPALRHVSLQTGVKHYASLTGSTGGGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 RR--VAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDG 240
           RR  VAW++LRPGLI+G SNR+FFNFMGSL VYGAICKKLK+PFVFGGTA CW EVYIDG
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240

Query: 241 SDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFG--AATEDVR 300
           SDARL A QH+WA TK + +N    GEAFNVCNGWSF WKEIWGAV EK G  A  ED R
Sbjct: 241 SDARLTAEQHVWATTKAVEINAITYGEAFNVCNGWSFTWKEIWGAVAEKLGVTAGAEDER 300

Query: 301 -FSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKAD 360
            FS EFRYTAAMA+KG +WAEIVEEEGLV TEM ELANWGFLDSLFRLPEKMVASR K+D
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 QFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           + GFT+K+ MLDS+ +WIDVMRHDKLIP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIP 388

[Close]

BLAST of MC03g0090 vs. NCBI nr
Match: KAG6583366.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619 bits (1596), Expect = 5.11e-221
Identity = 303/388 (78.09%), Postives = 337/388 (86.86%), Query Frame = 0


Query: 1   MAAETMRSG--GNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MA E+M +    NGELVAVIFGVTGLVGKELA  LL+TAGWKVYGVARRPDN+SPI+DPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKLLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           FHFISCDLLDRRS + NLSPL HVTH+FWITWAAQLRFDS++CS+QNKAMLA+ALDA+LP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLCHVTHMFWITWAAQLRFDSQDCSNQNKAMLANALDAILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERL-G 180
           +AP+LRHVSLQTGVKHY SLTG  GGGG   V+ EDSPR+ESGNNFYY LEDLLRERL G
Sbjct: 121 SAPALRHVSLQTGVKHYASLTGSTGGGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 RR--VAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDG 240
           RR  VAW++LRPGLI+G SNR+FFNFMGS+ VYGAICKKLK+PFVFGGT  CW EVYIDG
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSVCVYGAICKKLKMPFVFGGTGACWEEVYIDG 240

Query: 241 SDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFG--AATEDVR 300
           SDARL A QH+WAATK + +N   +GEAFNVCNGWSF WKEIWGAV EK G  A  ED R
Sbjct: 241 SDARLTAEQHVWAATKAVEINAITDGEAFNVCNGWSFTWKEIWGAVAEKLGVTAGAEDER 300

Query: 301 -FSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKAD 360
            FS EFRYTAAMA+KG +WAEIVEEEGLV TEM ELANWGFLDSLFRLPEKMVASR K+D
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 QFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           + GFT+K+ MLDS+ +WIDVMRHDKLIP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIP 388

[Close]

BLAST of MC03g0090 vs. NCBI nr
Match: XP_022964858.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 613 bits (1582), Expect = 6.93e-219
Identity = 301/388 (77.58%), Postives = 334/388 (86.08%), Query Frame = 0


Query: 1   MAAETMRSG--GNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MA E+M +    NGELVAVIFGVTGLVGKELA  LL+TAGWKVYGVARRPDN+SPI+DPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           FHFISCDLLDRRS + NLSPL HVTH+FWITWAAQLRFDS++CSDQNKAMLA+ALDA+LP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERL-G 180
           +AP+LRHVSLQTGVKHY SLT   G GG   V+ E+SPR+ESGNNFYY LEDLLRERL G
Sbjct: 121 SAPALRHVSLQTGVKHYASLTSSTGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 RR--VAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDG 240
           RR  VAW++LRPGLI+G SNR+FFNFMGSL VYGAICKKLK+PFVFGGTA CW EVYID 
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDS 240

Query: 241 SDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAAT--EDVR 300
           SDARL A QH+WAATK + +N   +GEAFNVCNGWSF WKEIWGAV EK G     ED R
Sbjct: 241 SDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 -FSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKAD 360
            FS EFRYTAAMA+KG +WAEIVEEEGLV TEM ELANWGFLDSLFRLPEKMVASR K+D
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 QFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           + GFT+K+ MLDS+ +WIDVMRHDKLIP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIP 388

[Close]

BLAST of MC03g0090 vs. ExPASy TrEMBL
Match: A0A6J1D9S9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Momordica charantia OX=3673 GN=LOC111018952 PE=3 SV=1)

HSP 1 Score: 786 bits (2029), Expect = 3.28e-287
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0


Query: 1   MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFH 60
           MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFH
Sbjct: 15  MAAETMRSGGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPNFH 74

Query: 61  FISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAA 120
           FISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAA
Sbjct: 75  FISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAA 134

Query: 121 PSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRV 180
           PSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRV
Sbjct: 135 PSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGRRV 194

Query: 181 AWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARL 240
           AWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARL
Sbjct: 195 AWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARL 254

Query: 241 VAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRY 300
           VAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRY
Sbjct: 255 VAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRY 314

Query: 301 TAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKH 360
           TAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKH
Sbjct: 315 TAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKH 374

Query: 361 NMLDSMFHWIDVMRHDKLIP 380
           NMLDSMFHWIDVMRHDKLIP
Sbjct: 375 NMLDSMFHWIDVMRHDKLIP 394

[Close]

BLAST of MC03g0090 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 613 bits (1582), Expect = 3.35e-219
Identity = 301/388 (77.58%), Postives = 334/388 (86.08%), Query Frame = 0


Query: 1   MAAETMRSG--GNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MA E+M +    NGELVAVIFGVTGLVGKELA  LL+TAGWKVYGVARRPDN+SPI+DPN
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           FHFISCDLLDRRS + NLSPL HVTH+FWITWAAQLRFDS++CSDQNKAMLA+ALDA+LP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERL-G 180
           +AP+LRHVSLQTGVKHY SLT   G GG   V+ E+SPR+ESGNNFYY LEDLLRERL G
Sbjct: 121 SAPALRHVSLQTGVKHYASLTSSTGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 RR--VAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDG 240
           RR  VAW++LRPGLI+G SNR+FFNFMGSL VYGAICKKLK+PFVFGGTA CW EVYID 
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDS 240

Query: 241 SDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAAT--EDVR 300
           SDARL A QH+WAATK + +N   +GEAFNVCNGWSF WKEIWGAV EK G     ED R
Sbjct: 241 SDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 -FSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKAD 360
            FS EFRYTAAMA+KG +WAEIVEEEGLV TEM ELANWGFLDSLFRLPEKMVASR K+D
Sbjct: 301 MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSD 360

Query: 361 QFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           + GFT+K+ MLDS+ +WIDVMRHDKLIP
Sbjct: 361 RLGFTVKYKMLDSILYWIDVMRHDKLIP 388

[Close]

BLAST of MC03g0090 vs. ExPASy TrEMBL
Match: A0A6J1GA93 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452342 PE=3 SV=1)

HSP 1 Score: 613 bits (1582), Expect = 3.48e-219
Identity = 300/391 (76.73%), Postives = 335/391 (85.68%), Query Frame = 0


Query: 1   MAAETMRS--GGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MAAET+ +   GNGELVAVIFGVTGLVGKELA ALL+T GWKVYGVARRPDNVSPI D N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           F FISCDLLDR S +RNLSP+RHVTHVFW+TWAAQLRFD+ +CSDQN+ MLA+ LD +LP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLTGG--GGGGGESAVFCEDSPRSESGNNFYYALEDLLRERL 180
           AAP+LRHVSLQTG+KHYVSLTG   GGGGG  AV+CEDSPR+ESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 ---GRRVAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYI 240
              GRRVAW+VLRPGLI+G SNR+FFNFMGSL VYGAICKKLK PFVFGG+A CW    I
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 DGSDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAAT---- 300
           +GSDARLVA QH+WAAT  +  N T +GEAFNVCNGWSF WKEIWGAV EKFG A     
Sbjct: 241 NGSDARLVAEQHVWAATNEI--NTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAED 300

Query: 301 EDVRFSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQ 360
           E+  FS EF YTAAM +KG+LWAEIVEEEGL+PTEMEELANWGFLDSLFRLP KMVASR 
Sbjct: 301 EEGMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360

Query: 361 KADQFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           K+D+ GF++K+ MLDS+ +WIDVMR+DKLIP
Sbjct: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDKLIP 389

[Close]

BLAST of MC03g0090 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 1.70e-218
Identity = 300/395 (75.95%), Postives = 335/395 (84.81%), Query Frame = 0


Query: 1   MAAETMRSG--GNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MA E + +    NGELVAVIFGVTGLVGK+LA  LL+TAGWKVYGVARRPDNVSPI+ P 
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           FHFISCDLLDRRS  +NLSP+RHVTH+FWITWAAQLR DS +CSDQN+AMLA+ALDA+LP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLT----GGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRE 180
           +AP+LRHVSLQTG+KHY SLT    G GGGG    V+CEDSPR+ESGNNFYY LEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RL--GRR-VAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEV 240
           RL  GRR VAW+VLRPGLI+G SNR+FFNFMGSL VYGAICKKLK+PFVFGGTA CW EV
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 YIDGSDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAAT-- 300
           YIDGSDARL A QHIW ATK + +N T +GEAFNVCNGWSF+WKEIWGAV EK G AT  
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 ----EDVRFSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMV 360
               ++  FS EF YTAAM DKGK+WAEIVEEEGLV TEMEELANWGFLD+LFRLPEKMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRQKADQFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           ASR K+D+ GF +K+ MLDSM +WIDVMR+DKLIP
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIP 395

[Close]

BLAST of MC03g0090 vs. ExPASy TrEMBL
Match: A0A6J1K9J8 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492905 PE=3 SV=1)

HSP 1 Score: 607 bits (1566), Expect = 1.02e-216
Identity = 299/393 (76.08%), Postives = 335/393 (85.24%), Query Frame = 0


Query: 1   MAAETMRS--GGNGELVAVIFGVTGLVGKELAIALLATAGWKVYGVARRPDNVSPIADPN 60
           MAAET+ +   GNGELVAVIFGVTGLVGKELA  LL+T GWKVYGVARRPDNVSPI D N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FHFISCDLLDRRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLP 120
           F FISCDLLDR S +RNLSP+RHVTH+FW+TW AQLRFD+ +CSDQN+ MLA+ LD +LP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AAPSLRHVSLQTGVKHYVSLTGG---GGGGGESAVFCEDSPRSESGNNFYYALEDLLRER 180
           AAP+LRHVSLQTG+KHYVSLTG    GGGGG  AV+CEDSPR+ESGNNFYYALEDLLRER
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 L---GRRVAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVY 240
           L   GRRVAW+VLRPGLI+G SNR+FFNFMGSL VYGAICKKLK PFVFGG+A CW    
Sbjct: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240

Query: 241 IDGSDARLVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFG---AAT 300
           I+GSDARLVA QH+WAAT  +  N T +GEAFNVCNGWSF WKEIWGAV EKFG   AA 
Sbjct: 241 INGSDARLVAEQHVWAATNEI--NTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAA 300

Query: 301 EDVR--FSAEFRYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVAS 360
           ED +  FS EF YTAAMA+KGK+WAEIVEEEGL+PTEMEELANWGFLDSLFRLP KMVAS
Sbjct: 301 EDEKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVAS 360

Query: 361 RQKADQFGFTIKHNMLDSMFHWIDVMRHDKLIP 380
           R K+D+ GF++K+ MLDS+ +WIDVMR+D LIP
Sbjct: 361 RNKSDRLGFSVKYKMLDSILYWIDVMRYDTLIP 391

[Close]

BLAST of MC03g0090 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 392.5 bits (1007), Expect = 3.8e-109
Identity = 205/382 (53.66%), Postives = 259/382 (67.80%), Query Frame = 0


Query: 6   MRSGGNGELVAVIFGVTGLVGKELAIALL-ATAGWKVYGVARRPDNVSPIADPNFHFISC 65
           MR     E VA+IFGVTGLVG+E+   LL +  GW++YGVAR P+  S      ++FISC
Sbjct: 9   MRRNEVDENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPEINS--MTKMYNFISC 68

Query: 66  DLLDRRSTYRNLSPLRH-VTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSL 125
           DLL+   T + LSPL+  V+HVFW+TW+ +   D+ EC  QNK ML +ALDA+LP A  L
Sbjct: 69  DLLNASETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRL 128

Query: 126 RHVSLQTGVKHYVSL---TGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGR-R 185
           +H SLQTG+KHYVSL   T   G G     + E+ PR  SG NFYY LEDLL+E++ R  
Sbjct: 129 KHFSLQTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS 188

Query: 186 VAWTVLRPGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDAR 245
           V W+V RPGL++GSS+R+ +NFMGSL VYGA+CK L LPFVFGGT ECW E YIDGSD+ 
Sbjct: 189 VVWSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSN 248

Query: 246 LVAHQHIWAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGA-ATEDVRFSAEF 305
           LVA QHI+AAT         +GEAFN  NG  F WKEIW  +G+K G    E   F   F
Sbjct: 249 LVAEQHIFAATSG---KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGF 308

Query: 306 RYTAAMADKGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTI 365
            +   M ++  +W EIV +E LV TE+E+LANW FLD+LFR P K++  R+K D+FGF  
Sbjct: 309 WFGREMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKR 368

Query: 366 KHNMLDSMFHWIDVMRHDKLIP 381
           K+  LDS+ +WIDVMR +KLIP
Sbjct: 369 KYRTLDSVLYWIDVMRDEKLIP 385

[Close]

BLAST of MC03g0090 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.5e-65
Identity = 148/374 (39.57%), Postives = 203/374 (54.28%), Query Frame = 0


Query: 13  ELVAVIFGVTGLVGKELA--IALLATAG--WKVYGVARRPDNVSPIADPNFHFISCDLLD 72
           E VA+I GVTG+VG  LA  + L  T G  WKVYGVARRP   +  AD    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 73  RRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHVSL 132
              T   LSPL  VTHVF++TW  +   +S  C + N +ML + L A++P AP+LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 133 QTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGR--RVAWTVLR 192
           QTG KHY+       G      F ED PR +   NFYY  ED+L E + +   V W++ R
Sbjct: 146 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIKKIETVTWSIHR 205

Query: 193 PGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQHI 252
           P +I G S  S  N +G+L VY AICK    P +F G+ + W E ++  SDA L+A Q I
Sbjct: 206 PNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIAEQQI 265

Query: 253 WAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAMAD 312
           WAA     ++   + EAFN  N   FKWK +W  + E+FG                 M  
Sbjct: 266 WAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKG 325

Query: 313 KGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLDSM 372
           K ++W E+V+E  L   ++EE+  W F D +  + E M+ S  K+ ++GF    N  +S 
Sbjct: 326 KERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNSNNSF 385

Query: 373 FHWIDVMRHDKLIP 381
             WID  +  K++P
Sbjct: 386 ISWIDKYKAFKIVP 388

[Close]

BLAST of MC03g0090 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.5e-65
Identity = 148/374 (39.57%), Postives = 203/374 (54.28%), Query Frame = 0


Query: 13  ELVAVIFGVTGLVGKELA--IALLATAG--WKVYGVARRPDNVSPIADPNFHFISCDLLD 72
           E VA+I GVTG+VG  LA  + L  T G  WKVYGVARRP   +  AD    +I CD+ D
Sbjct: 25  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 84

Query: 73  RRSTYRNLSPLRHVTHVFWITWAAQLRFDSRECSDQNKAMLAHALDALLPAAPSLRHVSL 132
              T   LSPL  VTHVF++TW  +   +S  C + N +ML + L A++P AP+LRHV L
Sbjct: 85  AEDTRSKLSPLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCL 144

Query: 133 QTGVKHYVSLTGGGGGGGESAVFCEDSPRSESGNNFYYALEDLLRERLGR--RVAWTVLR 192
           QTG KHY+       G      F ED PR +   NFYY  ED+L E + +   V W++ R
Sbjct: 145 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIKKIETVTWSIHR 204

Query: 193 PGLIIGSSNRSFFNFMGSLSVYGAICKKLKLPFVFGGTAECWAEVYIDGSDARLVAHQHI 252
           P +I G S  S  N +G+L VY AICK    P +F G+ + W E ++  SDA L+A Q I
Sbjct: 205 PNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIAEQQI 264

Query: 253 WAATKHLPLNGTGEGEAFNVCNGWSFKWKEIWGAVGEKFGAATEDVRFSAEFRYTAAMAD 312
           WAA     ++   + EAFN  N   FKWK +W  + E+FG                 M  
Sbjct: 265 WAA-----VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKG 324

Query: 313 KGKLWAEIVEEEGLVPTEMEELANWGFLDSLFRLPEKMVASRQKADQFGFTIKHNMLDSM 372
           K ++W E+V+E  L   ++EE+  W F D +  + E M+ S  K+ ++GF    N  +S 
Sbjct: 325 KERVWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNSNNSF 384

Query: 373 FHWIDVMRHDKLIP 381
             WID  +  K++P
Sbjct: 385 ISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:

BLAST of MC03g0090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1C9CX66	4.8e-64	40.96	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q9STX2	6.3e-64	39.57	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
A0A221J5W8	1.2e-62	39.36	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ...	[more]
A0A221J5X3	1.2e-62	39.36	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
A0A221J5X6	1.7e-61	39.20	(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta racemosa OX=54731 GN=ISY2 PE=1...	[more]

BLAST of MC03g0090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150915.1	6.78e-287	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase [Momordica charantia]	[more]
XP_038895390.1	7.31e-224	78.12	(S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida]	[more]
KAG7019139.1	1.26e-221	78.61	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]
KAG6583366.1	5.11e-221	78.09	(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022964858.1	6.93e-219	77.58	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]

BLAST of MC03g0090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D9S9	3.28e-287	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1HM54	3.35e-219	77.58	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1GA93	3.48e-219	76.73	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0LVI2	1.70e-218	75.95	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P...	[more]
A0A6J1K9J8	1.02e-216	76.08	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of MC03g0090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58750.1	3.8e-109	53.66	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	4.5e-65	39.57	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	4.5e-65	39.57	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 14..380 e-value: 2.6E-101 score: 341.1
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 10..380
None	No IPR available	PANTHER	PTHR32487:SF12	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1	coord: 10..380
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 15..339 e-value: 2.63079E-97 score: 290.301
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 17..86 e-value: 7.1E-5 score: 22.4
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 15..372

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0090.1	MC03g0090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity