MC03g0085 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g0085 vs. ExPASy Swiss-Prot
Match: Q39033 (Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 4.4e-186
Identity = 328/587 (55.88%), Postives = 426/587 (72.57%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M KQ+++VCFCFRRRFR   +EAP +++ +F KYSENG+M +DHL RFL ++Q +   T+
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTG 120
             AQ+I N+   L    R GL L+ FF+YLFGD N   +  + V+ DM AP+SHY+IFTG
Sbjct: 61  EDAQSIINSASSL--LHRNGLHLDAFFKYLFGDNNPPLALHK-VHHDMDAPISHYFIFTG 120

Query: 121 HNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKC 180
           HNSYLTGNQLSSD S  PII AL +GVR IELD+WPNS K+DIDVLHG TLT PV LIKC
Sbjct: 121 HNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPR-SEYLNGFPSPE 240
           L+AI+ HAF  S+YP+V+T EDHLTPDL+ +VA MVT  FG++L+ P   E L  FPSP 
Sbjct: 181 LKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSPN 240

Query: 241 SLRRRILISTKPP-EHTKGESIKEKPSADKQRDTADDDIW----------ESVQQQQDMD 300
           SL+RRI+ISTKPP E+ +G+ ++      K +D  D+++W             + + D+D
Sbjct: 241 SLKRRIIISTKPPKEYKEGKDVE---VVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLD 300

Query: 301 -EDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELE 360
             D  +++++EDK + NA P+Y+ LIAIHAGK K G + + L  D +KV RLSLSE++LE
Sbjct: 301 GNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGIT-ECLKVDPDKVRRLSLSEEQLE 360

Query: 361 NAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIM 420
            A  K+ + I+RFT+ NLLR+YPKG R+ SSNYNP++GW+HGAQMVAFNMQGYG+ LW+M
Sbjct: 361 KAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLM 420

Query: 421 EGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHF 480
           +GMFR NGGCGYIKKPD+LLK+  D   +    +  + + L+V VYMGEGW+ +F  +HF
Sbjct: 421 QGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHF 480

Query: 481 DFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDY 540
           D YSPPD Y +VGI GV  DT  +KT  +ED WIP W+E F F ++ PELALL + V +Y
Sbjct: 481 DQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEY 540

Query: 541 DTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
           D S KDDF GQTCLPV EL  GIR+ PL+SRKGEK+K VKLL++ EF
Sbjct: 541 DMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 580

BLAST of MC03g0085 vs. ExPASy Swiss-Prot
Match: Q9LY51 (Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 3.8e-174
Identity = 320/592 (54.05%), Postives = 411/592 (69.43%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M KQ+++VCFCFRRR+R  V+ AP +++ +F  YS+ G+M  D L RFL ++Q +   TK
Sbjct: 1   MSKQTYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGLMTTDLLLRFLIDVQKQDKATK 60

Query: 61  --AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTG 120
             AQ I N    L    R GL L+ FF+YLF   N   S S  V+QDM APLSHY+I+TG
Sbjct: 61  EEAQDIVNASSSL--LHRNGLHLDAFFKYLFAVTNSPLS-SLEVHQDMDAPLSHYFIYTG 120

Query: 121 HNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKC 180
           HNSYLTGNQLSSD S  PII AL +GVR IELD+WPNS ++ IDVLHG TLT+PV+LIKC
Sbjct: 121 HNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPVELIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRS-EYLNGFPSPE 240
           LRAI++HAF  S+YP+V+T EDHLTP L+ +VA MVT  FG+ML+ P S E L  FPSP 
Sbjct: 181 LRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFPSPA 240

Query: 241 SLRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIW-----------------ESVQ 300
            L++RI+ISTKPP+  K  +  +     K RD  D ++W                 +S  
Sbjct: 241 FLKKRIMISTKPPKEYKAAT--DDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDKNDSNG 300

Query: 301 QQQDMDEDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLS 360
              D D+D  ++++ +DK + NA PEY+ LIAI AGK K G + + L  D +KV RLSLS
Sbjct: 301 DDDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPKGGIT-ECLKVDPDKVRRLSLS 360

Query: 361 EQELENAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGK 420
           E++LE A  K+ + I+RFT+RNLLRVYPKG R+ SSNYNP++ W+HGAQMVAFNMQG G+
Sbjct: 361 EEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNMQGLGR 420

Query: 421 YLWIMEGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEF 480
            LW+M+GMFRGNGGCGYIKKPD+LLK+    A +    +  + + L+V +YMGEGW+ +F
Sbjct: 421 SLWVMQGMFRGNGGCGYIKKPDLLLKS---NAVFDPEATLPVKTTLRVTIYMGEGWYYDF 480

Query: 481 GLSHFDFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNV 540
             +HFD YSPPD Y +VGI GV  DT  +KT  +ED WIP W+E F F ++ PELALL +
Sbjct: 481 PHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRI 540

Query: 541 VVRDYDTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
            V +YD S KDDF GQ CLPV ELR GIR+VPL ++ G K + VKLL+R EF
Sbjct: 541 EVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRLEF 583

BLAST of MC03g0085 vs. ExPASy Swiss-Prot
Match: Q39032 (Phosphoinositide phospholipase C 1 OS=Arabidopsis thaliana OX=3702 GN=PLC1 PE=2 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 3.3e-165
Identity = 289/574 (50.35%), Postives = 395/574 (68.82%), Query Frame = 0

Query: 3   KQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGE--SSDTK 62
           K+SF+VCFC  R F+   +E PE+++ +F  YS++  M+ D + RF+ ++QGE  +    
Sbjct: 2   KESFKVCFCCVRNFKVKSSEPPEEIKNLFHDYSQDDRMSADEMLRFVIQVQGETHADINY 61

Query: 63  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 122
            + IF+ LKH G+F  RG+ LE F+RYL  D N     ++ V+QDM  PLSHY+++TGHN
Sbjct: 62  VKDIFHRLKHHGVFHPRGIHLEGFYRYLLSDFNSPLPLTREVWQDMNQPLSHYFLYTGHN 121

Query: 123 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 182
           SYLTGNQL+S+SS+ PI++AL  GVR IELDLWPNS   + +V HGGTLT+   L KCL 
Sbjct: 122 SYLTGNQLNSNSSIEPIVKALRNGVRVIELDLWPNSSGKEAEVRHGGTLTSREDLQKCLN 181

Query: 183 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 242
            +K++AF  S YP+V+T EDHLTP L+K+VA+MV+ TFG  L+    E    FPSPESL+
Sbjct: 182 VVKENAFQVSAYPVVLTLEDHLTPILQKKVAKMVSKTFGGSLFQCTDETTECFPSPESLK 241

Query: 243 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 302
            +ILISTKPP+      I +  + D           ES + ++  D     EE+ +++DE
Sbjct: 242 NKILISTKPPKEYLQTQISKGSTTD-----------ESTRAKKISD----AEEQVQEEDE 301

Query: 303 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 362
            +   EYR LI+IHAG  K G     L  D  +V RLS+SEQ LE   +  G D+++FT+
Sbjct: 302 ESVAIEYRDLISIHAGNRKGGLK-NCLNGDPNRVIRLSMSEQWLETLAKTRGPDLVKFTQ 361

Query: 363 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 422
           RNLLR++PK  R DSSNY+P++GW HGAQMVAFNMQ +G+YLW+M+GMF+ NGGCGY+KK
Sbjct: 362 RNLLRIFPKTTRFDSSNYDPLVGWIHGAQMVAFNMQSHGRYLWMMQGMFKANGGCGYVKK 421

Query: 423 PDVLLKNLDDPASYSE-STSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGI 482
           PDVLL N  +   +   S +  I + LKVK+Y GEGW+++F L HFD YSPPD Y KVGI
Sbjct: 422 PDVLLSNGPEGEIFDPCSQNLPIKTTLKVKIYTGEGWNMDFPLDHFDRYSPPDFYAKVGI 481

Query: 483 VGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCL 542
            GV  DT + +T   +DEW P+W++EF F +  PEL+LL + V+DYD++ ++DFAGQTC 
Sbjct: 482 AGVPLDTASYRTEIDKDEWFPIWDKEFEFPLRVPELSLLCITVKDYDSNTQNDFAGQTCF 541

Query: 543 PVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 574
           P++E+R GIR+V L+ R GE +KHV+LLMRF  E
Sbjct: 542 PLSEVRPGIRAVRLHDRAGEVYKHVRLLMRFVLE 559

BLAST of MC03g0085 vs. ExPASy Swiss-Prot
Match: Q8GV43 (Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 SV=2)

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-163
Identity = 303/600 (50.50%), Postives = 397/600 (66.17%), Query Frame = 0

Query: 5   SFRVCFCFRRRFRTNVAEAPEDVEMMFRKYS-----------------ENGIMNIDHLQR 64
           ++R+   + R+F+ N     +DV   F +++                   G+M  + L  
Sbjct: 18  NYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCS 77

Query: 65  FLEEIQGESSDTKAQAIFNNL----KHLGIFQRRGLRLEDFFRYLF-GDLNLAFSPSQGV 124
           FL++    ++  +AQ + + +     H+  F R GL L+DFF +LF  DLN   +P   V
Sbjct: 78  FLDDHGESTTVAEAQRLIDEVIRRRHHVTRFTRHGLDLDDFFNFLFYDDLNPPITPH--V 137

Query: 125 YQDMGAPLSHYYIFTGHNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDID 184
           +QDM APLSHY+I+TGHNSYLTGNQLSSD S  P+I+AL RGVR IELDLWPNS   DI+
Sbjct: 138 HQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDIN 197

Query: 185 VLHGGTLTAPVKLIKCLRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDML 244
           VLHG TLT PV L+KCL++I+D+AF++S YP++IT EDHLTPDL+ +VA M T  FG ML
Sbjct: 198 VLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQML 257

Query: 245 YVPRSEYLNGFPSPESLRRRILISTKPPE---HTKGESIKEK------PSADKQRDTADD 304
           Y P S+ L  FPSP SL  RI+ISTKPP+    ++   +K+K       S D+   T + 
Sbjct: 258 YYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRTEEI 317

Query: 305 DIWESVQQQQDMDEDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEK 364
              ES+   QD +     ++E+E+  E+   P Y+ LI IHAGK K G+  + +   ++K
Sbjct: 318 QTLESMLFDQDFESKSDSDQEDEEASEDQK-PAYKRLITIHAGKPK-GTVKEEMKVVVDK 377

Query: 365 VSRLSLSEQELENAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAF 424
           V RLSLSEQEL+     + +D++RFT+RNLLR+YPKG R +SSNY P++GWTHGAQM+AF
Sbjct: 378 VRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQMIAF 437

Query: 425 NMQGYGKYLWIMEGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMG 484
           NMQGYGK LW+M GMFR NGGCGY+KKP+ L+K       +       +   LKVKVYMG
Sbjct: 438 NMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKETLKVKVYMG 497

Query: 485 EGWHLEFGLSHFDFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAP 544
           +GW ++F  +HFD YSPPD Y K+ IVGV  D   +KT  IED W P+W+EEF F ++ P
Sbjct: 498 DGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFSFPLTVP 557

Query: 545 ELALLNVVVRDYDTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 574
           ELALL + VR+YD S KDDF GQTCLPV ELR GIRSVPLY +KGEK K V+LLMRF FE
Sbjct: 558 ELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKMKSVRLLMRFIFE 613

BLAST of MC03g0085 vs. ExPASy Swiss-Prot
Match: Q944C1 (Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 SV=2)

HSP 1 Score: 557.4 bits (1435), Expect = 1.9e-157
Identity = 299/593 (50.42%), Postives = 391/593 (65.94%), Query Frame = 0

Query: 5   SFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSE-NGIMNIDHLQRFLEEIQGESSDTKAQA 64
           S++ C  F R+FR   +   EDV  +F KY+E +  M+ + LQ+ + E  GE   +  +A
Sbjct: 9   SYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLEEA 68

Query: 65  ------IFNNLKHLGIFQRRGLRLEDFFRYLFG-DLNLAFSPSQGVYQDMGAPLSHYYIF 124
                 +     H+  F RR L L+DF  YLF  DLN   +    V+Q+M APLSHY+IF
Sbjct: 69  ERIVDEVLRRKHHIAKFTRRNLTLDDFNYYLFSTDLNPPIADQ--VHQNMDAPLSHYFIF 128

Query: 125 TGHNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLI 184
           TGHNSYLTGNQLSS+ S  PI  AL RGVR +ELDLWP    +D+ V HG TLT  VKL 
Sbjct: 129 TGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRG-TDDVCVKHGRTLTKEVKLG 188

Query: 185 KCLRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSP 244
           KCL +IK +AF  S+YP++IT EDHLTP L+ +VA+M+T TFGDMLY   S+    FPSP
Sbjct: 189 KCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFPSP 248

Query: 245 ESLRRRILISTKPP-EHTKGESIKEKPSADKQRDTADDDIW-----ESVQQQQDMDE--- 304
           E L+ +ILISTKPP E+ +    KEK + +K +D +D+D+W     + +  Q D+D+   
Sbjct: 249 EELKEKILISTKPPKEYLEANDTKEKDNGEKGKD-SDEDVWGKEPEDLISTQSDLDKVTS 308

Query: 305 ---DHLEEEEEEDKDENNA-----VPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSL 364
              D  +++EE    E++       PEY+ LIAIHAGK K G  + AL  D  K+ RLSL
Sbjct: 309 SVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRM-ALKVDPNKIRRLSL 368

Query: 365 SEQELENAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYG 424
           SEQ LE A+  +G D+IRFT++N LR+YPKG R +SSNY P +GW  GAQM+AFNMQGYG
Sbjct: 369 SEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGYG 428

Query: 425 KYLWIMEGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLE 484
           + LW+MEGMFR NGGCGY+KKPD L+    +   +    +S+    LKVKV MG+GW L+
Sbjct: 429 RALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLLD 488

Query: 485 FGLSHFDFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLN 544
           F  +HFD YSPPD +V+VGI G   D    KT    D W P+WN+EF F ++ PELALL 
Sbjct: 489 FKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALLR 548

Query: 545 VVVRDYDTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
           V V ++D + KDDF GQTCLPV+E+R GIR+VPL++RKG K+   +LLMRFEF
Sbjct: 549 VEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRFEF 596

BLAST of MC03g0085 vs. NCBI nr
Match: XP_022150956.1 (phosphoinositide phospholipase C 2-like isoform X2 [Momordica charantia])

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK
Sbjct: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR
Sbjct: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
           RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE
Sbjct: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
           NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR
Sbjct: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV
Sbjct: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP
Sbjct: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE
Sbjct: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573

BLAST of MC03g0085 vs. NCBI nr
Match: XP_022150955.1 (phosphoinositide phospholipase C 2-like isoform X1 [Momordica charantia])

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 573/575 (99.65%), Postives = 573/575 (99.65%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK
Sbjct: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGG--TLTAPVKLIKC 180
           SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGG  TLTAPVKLIKC
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGCRTLTAPVKLIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES 240
           LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES
Sbjct: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES 240

Query: 241 LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK 300
           LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK
Sbjct: 241 LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK 300

Query: 301 DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF 360
           DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF
Sbjct: 301 DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF 360

Query: 361 TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI 420
           TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI
Sbjct: 361 TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI 420

Query: 421 KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG 480
           KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG
Sbjct: 421 KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG 480

Query: 481 IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC 540
           IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC
Sbjct: 481 IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC 540

Query: 541 LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE
Sbjct: 541 LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 575

BLAST of MC03g0085 vs. NCBI nr
Match: XP_038894759.1 (phosphoinositide phospholipase C 2-like [Benincasa hispida])

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 486/573 (84.82%), Postives = 517/573 (90.23%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYK SFRVCFCFRRRFRTNVAEAPEDV+MMF +YSENGIMNID L+ FLEEIQGE SD K
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKMMFNQYSENGIMNIDQLRMFLEEIQGEESDIK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           AQAIFNNLKHL IFQRRGL LEDFFRYL GDLNLAFSPSQGVYQDM APLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLNIFQRRGLHLEDFFRYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSSDSSVTPIIRAL RGVRAIELDLWP+SKKN IDVLHGGTLTAPV LIKCLR
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGGTLTAPVALIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYP+VITFEDHLT DLRKEVA+MVT TFGD+LYVP+SE LN FPSPESL+
Sbjct: 181 AIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTVTFGDILYVPKSEDLNEFPSPESLK 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
            RILISTKPPEH K ES KEKP ADKQRDTADDDIWESV+ Q+DMDEDHL EE   DKDE
Sbjct: 241 GRILISTKPPEHNKVESTKEKPPADKQRDTADDDIWESVRPQEDMDEDHLVEE---DKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
           +  +PEYRSLIAIHA KMK GS L+  FNDIEKVSRLSLSEQELENA+  +G DIIRFT+
Sbjct: 301 DIVIPEYRSLIAIHAKKMKRGSDLQTFFNDIEKVSRLSLSEQELENAVTNYGHDIIRFTQ 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMF+GN GCGYIKK
Sbjct: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFKGNDGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PD LL N D   S S STSS +I RLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVK+ IV
Sbjct: 421 PDFLLTNPDHKTSNSRSTSSTMIKRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKIRIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVR+DT TR+T  +ED+W+PVWNEEF FSIS PELALL +VVRD+DTSGKDDFAGQTCLP
Sbjct: 481 GVREDTVTRRTIPVEDQWVPVWNEEFSFSISTPELALLQLVVRDHDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           V ELRSGIR+VPLY+RKGE++KHVKLLMRFEFE
Sbjct: 541 VKELRSGIRAVPLYNRKGERYKHVKLLMRFEFE 570

BLAST of MC03g0085 vs. NCBI nr
Match: KAG6583361.1 (Phosphoinositide phospholipase C 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 942 bits (2435), Expect = 0.0
Identity = 473/573 (82.55%), Postives = 508/573 (88.66%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYK SFRVCFCFRRRFRTNVAEAPEDV+ +F +YSENG MNID L+ FLEE+QGE S+ K
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLRMFLEEVQGEESEMK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           A AIF+NLKHL IFQRRGLRLEDFFRYL GDLNLAFSPSQGVYQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFRYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSS SSVTPIIRAL RGVRAIELDLWP+SKKN IDVLHGGT TAPVKLIKCLR
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGGTFTAPVKLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYP+VITFEDHLT DLRKEVA+MVT TFGD+LYVP+SE LN FPSPESL+
Sbjct: 181 AIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPESLK 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
            RILISTKPPEHTK ES KEKPSADKQRDTADDDIWES   ++D DED+LEE+   DKDE
Sbjct: 241 GRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTDEDYLEED---DKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
              +PEYRSLIAIHA KMK GS+L+  FN+IEKVSRLSLSEQELENA    GRDIIRFT+
Sbjct: 301 EIVIPEYRSLIAIHAKKMKRGSNLQTFFNEIEKVSRLSLSEQELENATGNCGRDIIRFTQ 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKG RLDSSNYNPML WTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PD LL N +   S S STS  +I RLK+KVYMGEGW LEFG SHFDFYSPPDLYVK+GIV
Sbjct: 421 PDFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGHSHFDFYSPPDLYVKIGIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVR+DT  ++T  IED+W+PVWNEEF FSISAPELALL VVV D+DTSGKDDFAGQTCLP
Sbjct: 481 GVREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           V ELRSGIR+VPLY+RKGE +KHVKLLMRFEFE
Sbjct: 541 VRELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of MC03g0085 vs. NCBI nr
Match: XP_022964953.1 (phosphoinositide phospholipase C 2-like [Cucurbita moschata])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 473/573 (82.55%), Postives = 507/573 (88.48%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYK SFRVCFCFRRRFRTNVAEAPEDV+ +F +YSENG MNID LQ FLEE+QGE S+ K
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           A AIF+NLKHL IFQRRGLRLEDFF+YL GDLNLAFSPSQGVYQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFQYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSS SSVTPIIRAL RGVRAIELDLWP+SKKN IDVLHGGT TAPVKLIKCLR
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGGTFTAPVKLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYP+VITFEDHLT DLRKEVA+MVT TFGD+LYVP+SE LN FPSPESL+
Sbjct: 181 AIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPESLK 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
            RILISTKPPEHTK ES KEKPSADKQRDTADDDIWES   ++D DED+LEE+   DKDE
Sbjct: 241 GRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTDEDYLEED---DKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
              +PEYRSLIAIHA KMK GS+L+  FNDIEKVSRLSLSEQELENA    GRDIIRFT+
Sbjct: 301 EIVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQ 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKG RLDSSNYNPML WTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PD LL N +   S S STS  +I RLK+KVYMGEGW LEFG SHFDFYSPPDLYVK+ IV
Sbjct: 421 PDFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGHSHFDFYSPPDLYVKIRIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVR+DT  ++T  IED+W+PVWNEEF FSISAPELALL VVV D+DTSGKDDFAGQTCLP
Sbjct: 481 GVREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           V ELRSGIR+VPLY+RKGE +KHVKLLMRFEFE
Sbjct: 541 VRELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of MC03g0085 vs. ExPASy TrEMBL
Match: A0A6J1DD40 (Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111018981 PE=4 SV=1)

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK
Sbjct: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR
Sbjct: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
           RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE
Sbjct: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
           NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR
Sbjct: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV
Sbjct: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP
Sbjct: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE
Sbjct: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573

BLAST of MC03g0085 vs. ExPASy TrEMBL
Match: A0A6J1D9W9 (Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111018981 PE=4 SV=1)

HSP 1 Score: 1142 bits (2953), Expect = 0.0
Identity = 573/575 (99.65%), Postives = 573/575 (99.65%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK
Sbjct: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN
Sbjct: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGG--TLTAPVKLIKC 180
           SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGG  TLTAPVKLIKC
Sbjct: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGCRTLTAPVKLIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES 240
           LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES
Sbjct: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPES 240

Query: 241 LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK 300
           LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK
Sbjct: 241 LRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDK 300

Query: 301 DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF 360
           DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF
Sbjct: 301 DENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRF 360

Query: 361 TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI 420
           TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI
Sbjct: 361 TRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYI 420

Query: 421 KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG 480
           KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG
Sbjct: 421 KKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVG 480

Query: 481 IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC 540
           IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC
Sbjct: 481 IVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTC 540

Query: 541 LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE
Sbjct: 541 LPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 575

BLAST of MC03g0085 vs. ExPASy TrEMBL
Match: A0A6J1HMF1 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111464903 PE=4 SV=1)

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 473/573 (82.55%), Postives = 507/573 (88.48%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYK SFRVCFCFRRRFRTNVAEAPEDV+ +F +YSENG MNID LQ FLEE+QGE S+ K
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           A AIF+NLKHL IFQRRGLRLEDFF+YL GDLNLAFSPSQGVYQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFQYLLGDLNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSS SSVTPIIRAL RGVRAIELDLWP+SKKN IDVLHGGT TAPVKLIKCLR
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGIDVLHGGTFTAPVKLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYP+VITFEDHLT DLRKEVA+MVT TFGD+LYVP+SE LN FPSPESL+
Sbjct: 181 AIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPESLK 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
            RILISTKPPEHTK ES KEKPSADKQRDTADDDIWES   ++D DED+LEE+   DKDE
Sbjct: 241 GRILISTKPPEHTKVESTKEKPSADKQRDTADDDIWESAMSREDTDEDYLEED---DKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
              +PEYRSLIAIHA KMK GS+L+  FNDIEKVSRLSLSEQELENA    GRDIIRFT+
Sbjct: 301 EIVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQ 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKG RLDSSNYNPML WTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PD LL N +   S S STS  +I RLK+KVYMGEGW LEFG SHFDFYSPPDLYVK+ IV
Sbjct: 421 PDFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGHSHFDFYSPPDLYVKIRIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVR+DT  ++T  IED+W+PVWNEEF FSISAPELALL VVV D+DTSGKDDFAGQTCLP
Sbjct: 481 GVREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           V ELRSGIR+VPLY+RKGE +KHVKLLMRFEFE
Sbjct: 541 VRELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of MC03g0085 vs. ExPASy TrEMBL
Match: A0A6J1I4E1 (Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111469541 PE=4 SV=1)

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 472/573 (82.37%), Postives = 508/573 (88.66%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           MYK SFRVCFCFRRRFRTNVAEAPEDV+ +F +YSENG MNID LQ FLEE+QGE S+ K
Sbjct: 1   MYKHSFRVCFCFRRRFRTNVAEAPEDVKRVFDQYSENGTMNIDQLQMFLEEVQGEESEMK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           A AIF+NLKHL IFQRRGLRLEDFFRYL G+LNLAFSPSQGVYQDM APLSHYYIFTGHN
Sbjct: 61  AHAIFDNLKHLNIFQRRGLRLEDFFRYLLGELNLAFSPSQGVYQDMRAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQLSS SSVTPIIRAL RGVRAIELDLWP+SKKN I+VLHGGTLTAPVKLIKCLR
Sbjct: 121 SYLTGNQLSSASSVTPIIRALKRGVRAIELDLWPSSKKNGINVLHGGTLTAPVKLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYP+VITFEDHLT DLRKEVA+MVT TFGD+LYVP+SE LN FPSPESL+
Sbjct: 181 AIKDHAFTASEYPVVITFEDHLTHDLRKEVAKMVTTTFGDILYVPKSEDLNEFPSPESLK 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
            RILISTKPPEHTK ES KEKPSADK+RDTADDDIWES   ++D DED+LEE+   DKDE
Sbjct: 241 GRILISTKPPEHTKVESTKEKPSADKRRDTADDDIWESTMSREDTDEDYLEED---DKDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
              +PEYRSLIAIHA KMK GS+L+  FNDIEKVSRLSLSEQELENA    GRDIIRFT+
Sbjct: 301 EIVIPEYRSLIAIHAKKMKRGSNLQTFFNDIEKVSRLSLSEQELENATGNCGRDIIRFTQ 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKG RLDSSNYNPML WTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGSRLDSSNYNPMLAWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PD LL N +   S S STS  +I RLK+KVYMGEGW LEFG SHFDFYSPPDLYVK+ IV
Sbjct: 421 PDFLLNNSEQENSTSRSTSLIMIKRLKLKVYMGEGWDLEFGPSHFDFYSPPDLYVKIRIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVR+DT  ++T  IED+W+PVWNEEF FSISAPELALL VVV D+DTSGKDDFAGQTCLP
Sbjct: 481 GVREDTVVKRTIPIEDQWVPVWNEEFSFSISAPELALLQVVVLDHDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           V ELRSGIR+VPLY+RKGE +KHVKLLMRFEFE
Sbjct: 541 VRELRSGIRAVPLYNRKGEIYKHVKLLMRFEFE 570

BLAST of MC03g0085 vs. ExPASy TrEMBL
Match: A0A6J1GAW1 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111452350 PE=4 SV=1)

HSP 1 Score: 887 bits (2291), Expect = 0.0
Identity = 443/573 (77.31%), Postives = 490/573 (85.51%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M K SFRVCF F RRFRTN AEAPEDV+MMF KYS++G MNID L+ FLEEIQGE+S+TK
Sbjct: 1   MSKHSFRVCFFFSRRFRTNAAEAPEDVKMMFNKYSKHGTMNIDQLRTFLEEIQGETSETK 60

Query: 61  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 120
           A+ IFN+LKHL IFQRRGL LEDFFRYL GDLNLAF   QGVYQDMGAPLSHYYIFTGHN
Sbjct: 61  AEDIFNSLKHLNIFQRRGLYLEDFFRYLLGDLNLAFPHCQGVYQDMGAPLSHYYIFTGHN 120

Query: 121 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 180
           SYLTGNQL S SSVTPIIRAL RGVRAIELDLWP SK N I+V HG TLT+PV LIKCLR
Sbjct: 121 SYLTGNQLYSKSSVTPIIRALKRGVRAIELDLWPTSKNNGIEVRHGRTLTSPVSLIKCLR 180

Query: 181 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 240
           AIKDHAFTASEYP+VITFEDHL P+LRKEVA+MV  TF DMLYVP  E LN   SPESL+
Sbjct: 181 AIKDHAFTASEYPVVITFEDHLNPELRKEVAKMVNETFEDMLYVPELEDLNKLRSPESLK 240

Query: 241 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 300
           +R+LIS KPP++ KGES KEKPS DKQRD ADDDIW SV+ Q+D+DED LEE    D+DE
Sbjct: 241 KRVLISAKPPKNKKGESTKEKPSTDKQRDIADDDIWGSVRPQEDVDEDDLEE----DEDE 300

Query: 301 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 360
           + AVPEYR LIAIHA K K G +L+A  ND E VSR SLSEQ LE A + +GRDIIRFT+
Sbjct: 301 DVAVPEYRKLIAIHAKKKKPGLNLQAFLNDTESVSRFSLSEQALEKATKDYGRDIIRFTQ 360

Query: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 420
           RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIM+GMFRGNGGCGYIKK
Sbjct: 361 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMQGMFRGNGGCGYIKK 420

Query: 421 PDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGIV 480
           PD LL NLD   S S S+SS +I RL +KVYMGEGW+ EFGLSHFDFYSPPDLYV++GIV
Sbjct: 421 PDFLLNNLDHKPSNSRSSSSTMIRRLTIKVYMGEGWNSEFGLSHFDFYSPPDLYVQIGIV 480

Query: 481 GVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCLP 540
           GVR DT T +T+ I+D+W+PVWN +F FSIS PELALL V+VRD+DTSGKDDFAGQTCLP
Sbjct: 481 GVRDDTTTERTATIQDQWVPVWNAKFSFSISTPELALLRVIVRDHDTSGKDDFAGQTCLP 540

Query: 541 VTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 573
           V ELRSGIR+VPLYS++GEK+KHVKLLM FEFE
Sbjct: 541 VKELRSGIRAVPLYSKRGEKYKHVKLLMSFEFE 569

BLAST of MC03g0085 vs. TAIR 10
Match: AT3G08510.1 (phospholipase C 2 )

HSP 1 Score: 652.5 bits (1682), Expect = 3.1e-187
Identity = 328/587 (55.88%), Postives = 426/587 (72.57%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M KQ+++VCFCFRRRFR   +EAP +++ +F KYSENG+M +DHL RFL ++Q +   T+
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTG 120
             AQ+I N+   L    R GL L+ FF+YLFGD N   +  + V+ DM AP+SHY+IFTG
Sbjct: 61  EDAQSIINSASSL--LHRNGLHLDAFFKYLFGDNNPPLALHK-VHHDMDAPISHYFIFTG 120

Query: 121 HNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKC 180
           HNSYLTGNQLSSD S  PII AL +GVR IELD+WPNS K+DIDVLHG TLT PV LIKC
Sbjct: 121 HNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPR-SEYLNGFPSPE 240
           L+AI+ HAF  S+YP+V+T EDHLTPDL+ +VA MVT  FG++L+ P   E L  FPSP 
Sbjct: 181 LKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSPN 240

Query: 241 SLRRRILISTKPP-EHTKGESIKEKPSADKQRDTADDDIW----------ESVQQQQDMD 300
           SL+RRI+ISTKPP E+ +G+ ++      K +D  D+++W             + + D+D
Sbjct: 241 SLKRRIIISTKPPKEYKEGKDVE---VVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLD 300

Query: 301 -EDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELE 360
             D  +++++EDK + NA P+Y+ LIAIHAGK K G + + L  D +KV RLSLSE++LE
Sbjct: 301 GNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGIT-ECLKVDPDKVRRLSLSEEQLE 360

Query: 361 NAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIM 420
            A  K+ + I+RFT+ NLLR+YPKG R+ SSNYNP++GW+HGAQMVAFNMQGYG+ LW+M
Sbjct: 361 KAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLM 420

Query: 421 EGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHF 480
           +GMFR NGGCGYIKKPD+LLK+  D   +    +  + + L+V VYMGEGW+ +F  +HF
Sbjct: 421 QGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHF 480

Query: 481 DFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDY 540
           D YSPPD Y +VGI GV  DT  +KT  +ED WIP W+E F F ++ PELALL + V +Y
Sbjct: 481 DQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEY 540

Query: 541 DTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
           D S KDDF GQTCLPV EL  GIR+ PL+SRKGEK+K VKLL++ EF
Sbjct: 541 DMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 580

BLAST of MC03g0085 vs. TAIR 10
Match: AT3G08510.2 (phospholipase C 2 )

HSP 1 Score: 652.5 bits (1682), Expect = 3.1e-187
Identity = 328/587 (55.88%), Postives = 426/587 (72.57%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M KQ+++VCFCFRRRFR   +EAP +++ +F KYSENG+M +DHL RFL ++Q +   T+
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTG 120
             AQ+I N+   L    R GL L+ FF+YLFGD N   +  + V+ DM AP+SHY+IFTG
Sbjct: 61  EDAQSIINSASSL--LHRNGLHLDAFFKYLFGDNNPPLALHK-VHHDMDAPISHYFIFTG 120

Query: 121 HNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKC 180
           HNSYLTGNQLSSD S  PII AL +GVR IELD+WPNS K+DIDVLHG TLT PV LIKC
Sbjct: 121 HNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPR-SEYLNGFPSPE 240
           L+AI+ HAF  S+YP+V+T EDHLTPDL+ +VA MVT  FG++L+ P   E L  FPSP 
Sbjct: 181 LKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSPN 240

Query: 241 SLRRRILISTKPP-EHTKGESIKEKPSADKQRDTADDDIW----------ESVQQQQDMD 300
           SL+RRI+ISTKPP E+ +G+ ++      K +D  D+++W             + + D+D
Sbjct: 241 SLKRRIIISTKPPKEYKEGKDVE---VVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLD 300

Query: 301 -EDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELE 360
             D  +++++EDK + NA P+Y+ LIAIHAGK K G + + L  D +KV RLSLSE++LE
Sbjct: 301 GNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGIT-ECLKVDPDKVRRLSLSEEQLE 360

Query: 361 NAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIM 420
            A  K+ + I+RFT+ NLLR+YPKG R+ SSNYNP++GW+HGAQMVAFNMQGYG+ LW+M
Sbjct: 361 KAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLM 420

Query: 421 EGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHF 480
           +GMFR NGGCGYIKKPD+LLK+  D   +    +  + + L+V VYMGEGW+ +F  +HF
Sbjct: 421 QGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHF 480

Query: 481 DFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDY 540
           D YSPPD Y +VGI GV  DT  +KT  +ED WIP W+E F F ++ PELALL + V +Y
Sbjct: 481 DQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEY 540

Query: 541 DTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
           D S KDDF GQTCLPV EL  GIR+ PL+SRKGEK+K VKLL++ EF
Sbjct: 541 DMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 580

BLAST of MC03g0085 vs. TAIR 10
Match: AT3G55940.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 612.8 bits (1579), Expect = 2.7e-175
Identity = 320/592 (54.05%), Postives = 411/592 (69.43%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M KQ+++VCFCFRRR+R  V+ AP +++ +F  YS+ G+M  D L RFL ++Q +   TK
Sbjct: 1   MSKQTYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGLMTTDLLLRFLIDVQKQDKATK 60

Query: 61  --AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTG 120
             AQ I N    L    R GL L+ FF+YLF   N   S S  V+QDM APLSHY+I+TG
Sbjct: 61  EEAQDIVNASSSL--LHRNGLHLDAFFKYLFAVTNSPLS-SLEVHQDMDAPLSHYFIYTG 120

Query: 121 HNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKC 180
           HNSYLTGNQLSSD S  PII AL +GVR IELD+WPNS ++ IDVLHG TLT+PV+LIKC
Sbjct: 121 HNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPVELIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRS-EYLNGFPSPE 240
           LRAI++HAF  S+YP+V+T EDHLTP L+ +VA MVT  FG+ML+ P S E L  FPSP 
Sbjct: 181 LRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFPSPA 240

Query: 241 SLRRRILISTKPPEHTKGESIKEKPSADKQRDTADDDIW-----------------ESVQ 300
            L++RI+ISTKPP+  K  +  +     K RD  D ++W                 +S  
Sbjct: 241 FLKKRIMISTKPPKEYKAAT--DDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDKNDSNG 300

Query: 301 QQQDMDEDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLS 360
              D D+D  ++++ +DK + NA PEY+ LIAI AGK K G + + L  D +KV RLSLS
Sbjct: 301 DDDDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPKGGIT-ECLKVDPDKVRRLSLS 360

Query: 361 EQELENAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGK 420
           E++LE A  K+ + I+RFT+RNLLRVYPKG R+ SSNYNP++ W+HGAQMVAFNMQG G+
Sbjct: 361 EEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNMQGLGR 420

Query: 421 YLWIMEGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEF 480
            LW+M+GMFRGNGGCGYIKKPD+LLK+    A +    +  + + L+V +YMGEGW+ +F
Sbjct: 421 SLWVMQGMFRGNGGCGYIKKPDLLLKS---NAVFDPEATLPVKTTLRVTIYMGEGWYYDF 480

Query: 481 GLSHFDFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNV 540
             +HFD YSPPD Y +VGI GV  DT  +KT  +ED WIP W+E F F ++ PELALL +
Sbjct: 481 PHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRI 540

Query: 541 VVRDYDTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
            V +YD S KDDF GQ CLPV ELR GIR+VPL ++ G K + VKLL+R EF
Sbjct: 541 EVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRLEF 583

BLAST of MC03g0085 vs. TAIR 10
Match: AT3G08510.3 (phospholipase C 2 )

HSP 1 Score: 594.0 bits (1530), Expect = 1.3e-169
Identity = 312/587 (53.15%), Postives = 405/587 (68.99%), Query Frame = 0

Query: 1   MYKQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGESSDTK 60
           M KQ+++VCFCFRRRFR   +EAP +++ +F KYSENG+M +DHL RFL ++Q +   T+
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  --AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTG 120
             AQ+I N+   L    R GL L+ FF+YLFGD N   +  + V+ DM AP+SHY+IFTG
Sbjct: 61  EDAQSIINSASSL--LHRNGLHLDAFFKYLFGDNNPPLALHK-VHHDMDAPISHYFIFTG 120

Query: 121 HNSYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKC 180
           HNSYLTGNQLSSD S  PII AL +GVR IELD+WPNS K+DIDVLHG TLT PV LIKC
Sbjct: 121 HNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIKC 180

Query: 181 LRAIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPR-SEYLNGFPSPE 240
           L+AI+ HAF  S+YP+V+T EDHLTPDL+ +VA MVT  FG++L+ P   E L  FPSP 
Sbjct: 181 LKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSPN 240

Query: 241 SLRRRILISTKPP-EHTKGESIKEKPSADKQRDTADDDIW----------ESVQQQQDMD 300
           SL+RRI+ISTKPP E+ +G+ ++      K +D  D+++W             + + D+D
Sbjct: 241 SLKRRIIISTKPPKEYKEGKDVE---VVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLD 300

Query: 301 -EDHLEEEEEEDKDENNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELE 360
             D  +++++EDK + NA P+Y+ LIAIHAGK K G + + L  D +KV RLSLSE++LE
Sbjct: 301 GNDDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGIT-ECLKVDPDKVRRLSLSEEQLE 360

Query: 361 NAIRKHGRDIIRFTRRNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIM 420
            A  K+ + I+RFT+ NLLR+YPKG R+ SSNYNP++GW+HGAQMVAFNMQGYG+ LW+M
Sbjct: 361 KAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLM 420

Query: 421 EGMFRGNGGCGYIKKPDVLLKNLDDPASYSESTSSAIISRLKVKVYMGEGWHLEFGLSHF 480
           +GMFR NGGCGYIKKPD+LLK+  D   +    +      L VK  +             
Sbjct: 421 QGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKAT------LPVKTTL------------- 480

Query: 481 DFYSPPDLYVKVGIVGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDY 540
                     +VGI GV  DT  +KT  +ED WIP W+E F F ++ PELALL + V +Y
Sbjct: 481 ----------RVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEY 540

Query: 541 DTSGKDDFAGQTCLPVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEF 573
           D S KDDF GQTCLPV EL  GIR+ PL+SRKGEK+K VKLL++ EF
Sbjct: 541 DMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 551

BLAST of MC03g0085 vs. TAIR 10
Match: AT5G58670.1 (phospholipase C1 )

HSP 1 Score: 583.2 bits (1502), Expect = 2.3e-166
Identity = 289/574 (50.35%), Postives = 395/574 (68.82%), Query Frame = 0

Query: 3   KQSFRVCFCFRRRFRTNVAEAPEDVEMMFRKYSENGIMNIDHLQRFLEEIQGE--SSDTK 62
           K+SF+VCFC  R F+   +E PE+++ +F  YS++  M+ D + RF+ ++QGE  +    
Sbjct: 2   KESFKVCFCCVRNFKVKSSEPPEEIKNLFHDYSQDDRMSADEMLRFVIQVQGETHADINY 61

Query: 63  AQAIFNNLKHLGIFQRRGLRLEDFFRYLFGDLNLAFSPSQGVYQDMGAPLSHYYIFTGHN 122
            + IF+ LKH G+F  RG+ LE F+RYL  D N     ++ V+QDM  PLSHY+++TGHN
Sbjct: 62  VKDIFHRLKHHGVFHPRGIHLEGFYRYLLSDFNSPLPLTREVWQDMNQPLSHYFLYTGHN 121

Query: 123 SYLTGNQLSSDSSVTPIIRALNRGVRAIELDLWPNSKKNDIDVLHGGTLTAPVKLIKCLR 182
           SYLTGNQL+S+SS+ PI++AL  GVR IELDLWPNS   + +V HGGTLT+   L KCL 
Sbjct: 122 SYLTGNQLNSNSSIEPIVKALRNGVRVIELDLWPNSSGKEAEVRHGGTLTSREDLQKCLN 181

Query: 183 AIKDHAFTASEYPLVITFEDHLTPDLRKEVARMVTATFGDMLYVPRSEYLNGFPSPESLR 242
            +K++AF  S YP+V+T EDHLTP L+K+VA+MV+ TFG  L+    E    FPSPESL+
Sbjct: 182 VVKENAFQVSAYPVVLTLEDHLTPILQKKVAKMVSKTFGGSLFQCTDETTECFPSPESLK 241

Query: 243 RRILISTKPPEHTKGESIKEKPSADKQRDTADDDIWESVQQQQDMDEDHLEEEEEEDKDE 302
            +ILISTKPP+      I +  + D           ES + ++  D     EE+ +++DE
Sbjct: 242 NKILISTKPPKEYLQTQISKGSTTD-----------ESTRAKKISD----AEEQVQEEDE 301

Query: 303 NNAVPEYRSLIAIHAGKMKSGSSLKALFNDIEKVSRLSLSEQELENAIRKHGRDIIRFTR 362
            +   EYR LI+IHAG  K G     L  D  +V RLS+SEQ LE   +  G D+++FT+
Sbjct: 302 ESVAIEYRDLISIHAGNRKGGLK-NCLNGDPNRVIRLSMSEQWLETLAKTRGPDLVKFTQ 361

Query: 363 RNLLRVYPKGLRLDSSNYNPMLGWTHGAQMVAFNMQGYGKYLWIMEGMFRGNGGCGYIKK 422
           RNLLR++PK  R DSSNY+P++GW HGAQMVAFNMQ +G+YLW+M+GMF+ NGGCGY+KK
Sbjct: 362 RNLLRIFPKTTRFDSSNYDPLVGWIHGAQMVAFNMQSHGRYLWMMQGMFKANGGCGYVKK 421

Query: 423 PDVLLKNLDDPASYSE-STSSAIISRLKVKVYMGEGWHLEFGLSHFDFYSPPDLYVKVGI 482
           PDVLL N  +   +   S +  I + LKVK+Y GEGW+++F L HFD YSPPD Y KVGI
Sbjct: 422 PDVLLSNGPEGEIFDPCSQNLPIKTTLKVKIYTGEGWNMDFPLDHFDRYSPPDFYAKVGI 481

Query: 483 VGVRKDTETRKTSAIEDEWIPVWNEEFCFSISAPELALLNVVVRDYDTSGKDDFAGQTCL 542
            GV  DT + +T   +DEW P+W++EF F +  PEL+LL + V+DYD++ ++DFAGQTC 
Sbjct: 482 AGVPLDTASYRTEIDKDEWFPIWDKEFEFPLRVPELSLLCITVKDYDSNTQNDFAGQTCF 541

Query: 543 PVTELRSGIRSVPLYSRKGEKFKHVKLLMRFEFE 574
           P++E+R GIR+V L+ R GE +KHV+LLMRF  E
Sbjct: 542 PLSEVRPGIRAVRLHDRAGEVYKHVRLLMRFVLE 559