MC03g0061 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g0061 vs. ExPASy Swiss-Prot
Match: Q80UJ7 (Rab3 GTPase-activating protein catalytic subunit OS=Mus musculus OX=10090 GN=Rab3gap1 PE=1 SV=4)

HSP 1 Score: 189.5 bits (480), Expect = 1.6e-46
Identity = 200/789 (25.35%), Postives = 325/789 (41.19%), Query Frame = 0

Query: 43  REFLCKLVDWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSS 102
           ++ LC   D+    H L   +G++EF+VIAP + S  +L   + + LLS+++IAL N   
Sbjct: 102 QDLLCMNNDFPPRAHCLVRWYGLREFVVIAPAAHSDAVLSESKCNLLLSSISIALGNTGC 161

Query: 103 FWPAFVPVHDPSRKAYIGIQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYS 162
             P FV +H   R+ Y+G +  G      FE   +  +VP +  HL GL ++F SK    
Sbjct: 162 QVPLFVQIHHKWRRMYMG-ECQGPGVRTDFEMVHL-RKVPSQYTHLSGLLDIFKSKIGCP 221

Query: 163 SVDISMNLFKVNFTMKLTFRVLLSDDDNIPRADAEMTESEHESAVGTR-GKVQWDDDCPW 222
              +      V+  ++LT+ +        P+   ++         G   GK+ +      
Sbjct: 222 LTPLP----PVSIAIRLTYVLQDWQQYFWPQQPPDIDALVGGEVGGLEFGKLPFG----- 281

Query: 223 SEWYSSEDPVKGFELIASWSEKMVESSLEMAELENSSPHEAKKWILFPILSPN------- 282
               + EDP+    L  +W        ++     +  P +A  W +    + N       
Sbjct: 282 ----ACEDPISELHLATTWPHLTEGIIVDNDVYSDLDPVQAPHWSVRVRKADNPQCLLGD 341

Query: 283 -------------ITDSTMG---------NIVGFASQLYLLIDALDVSFQAQFIEDFVSI 342
                         TD  +G          +      L  L +   V      + + V  
Sbjct: 342 FVTEFLKICRRKESTDEILGRSTFEEEGREVADITHALSKLTEPAPVPIHKLSVSNMVHT 401

Query: 343 ------ENPGSDNLKSSTVLP-------PPTVIDRVL--KEIFHEGKKSPSKGENRTSQA 402
                 ++ G ++  +S VL        P  V ++ L           +P  G+      
Sbjct: 402 AKKKIRKHRGEESPLNSDVLNTILLFLFPDAVSEKPLDGTTSIDNSIPAPEAGDYTLYNQ 461

Query: 403 IKAAPLDSLFTR--FCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSI 462
            K+AP DSL  +   CL ++ F +  ++ +A LW EFV E+R+ WE    +P +  S S 
Sbjct: 462 FKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGL-ASGSP 521

Query: 463 DLSTCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLIGTRVKPTSSKKELGHLSS 522
           DL  CL++QKLQML  CIE+K    E  +  L S    S   G   K T  +  L HL  
Sbjct: 522 DLRCCLLHQKLQMLNCCIERKKARDEGKKTSL-SDSTTSAYPGDAGK-TGGQLGLDHLRD 581

Query: 523 EDE------------SESKANLFRYSTKSEDVISITDQK----SPDSMR----RGSAGMV 582
            ++            S+S+   F   + +ED+     +      P  M      G     
Sbjct: 582 TEKEKGEVGKSWDSWSDSEEEFFECLSDTEDLKGNGQESGKKGGPKEMANLKPEGRLHQH 641

Query: 583 GTMMLLNSYQSMHAPFTQNAPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDM 642
           G + LL++ + ++ P TQ    MTED+ EE+ + +   G S +     A+++   L SDM
Sbjct: 642 GKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDM 701

Query: 643 SAFKAANPDGVFEDFIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSE 702
            +FKAANP    EDF+RW+SP D+ ++  + ++G            N   +G LS RM  
Sbjct: 702 ESFKAANPGCFLEDFVRWYSPRDYIEEEVTDEKG------------NVVLKGELSARMKI 761

Query: 703 HGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIV-CTAFRAAAD 761
             N+W + W  A  +P   Q+ L D  RE EK+LHYL   +P  L   ++ C    A   
Sbjct: 762 PSNMWVEAWETAKPVPARRQRRLFDDTREAEKVLHYLAMQKPADLARHLLPCVIHAAVLK 821

BLAST of MC03g0061 vs. ExPASy Swiss-Prot
Match: Q6NUV0 (Rab3 GTPase-activating protein catalytic subunit OS=Danio rerio OX=7955 GN=rab3gap1 PE=2 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 4.8e-46
Identity = 207/774 (26.74%), Postives = 334/774 (43.15%), Query Frame = 0

Query: 43  REFLCKLVDWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSS 102
           ++ LC   D+    H L   FGV+EF+VI+P +    I+   + S LLS+V+IAL N   
Sbjct: 102 QDLLCINNDFPPRAHCLVRWFGVREFVVISPGANCEAIISESKCSLLLSSVSIALANTGC 161

Query: 103 FWPAFVPVHDPSRKAYIGIQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYS 162
             P FV +    RK + G +  G      FE   +  +VP +  HL GL ++F +K    
Sbjct: 162 QVPLFVQIQQKHRKMFSG-ECQGPGVRSDFEMVHL-RRVPSQYNHLSGLLDIFKNKIGCP 221

Query: 163 SVDISMNLFKVNFTMKLTFRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWS 222
              +      + FT  L      S     P  DA +         G  G V++    P+ 
Sbjct: 222 LTPLPPINISIRFTYVLQDWQQCSWPQQPPDFDALL--------AGEVGGVEF-GKLPFG 281

Query: 223 EWYSSEDPVKGFELIASW---SEKMVESSLEMAELEN-SSPH--------EAKKWILFPI 282
              + EDP+    L A+W   +E +V  +   ++L+   +PH        E  + +L   
Sbjct: 282 ---ACEDPISELHLAATWPSLTEGIVVDNDVYSDLDPLQAPHWSVRVRTAENPQCLLGEF 341

Query: 283 LS--------PNITDSTMG------------NIVGFASQL-----YLLIDALDVSFQAQF 342
           L+           T+  +G            +I    S+L      + I  L VS     
Sbjct: 342 LTEFFKICCRKETTEEILGRSTAEEEGKENSDITQALSKLTEPSTAVPIHKLSVSSMVHS 401

Query: 343 IEDFVS-----IENPGSDNLKSSTVLP--PPTVIDRVLKEIFHEGKKSPSK--GENRTSQ 402
               +       E+P ++++ +S +L   P   +D+         ++SP +   +     
Sbjct: 402 ARKRIRRHRRVPESPLNNDVLNSILLYLFPDAALDKADVSEVRPPQQSPDRQSEDYHLYN 461

Query: 403 AIKAAPLDSLFTR--FCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSS 462
            +K+ P DSL  R   C+ ++ F +  +RA+A LW EFV E+R+ WE    L     S  
Sbjct: 462 QLKSCPSDSLTHRLALCICMVNFHHGGVRAVAHLWQEFVLEMRYRWEN-NCLIYGLASGP 521

Query: 463 IDLSTCLVNQKLQMLAICIEKK--HQSSEEFQDCLGSQDPDSPLIGTRVKPTSSKKELGH 522
            DL  CL++QKLQML  CIE+K      ++     G++D             +   E   
Sbjct: 522 PDLRCCLLHQKLQMLNCCIERKKARDDGKKSSSSDGARDRSRGAPEGAGPEGAGPAEAAG 581

Query: 523 LSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRR--GSAGMVGTMMLLNSYQSMHAP 582
            S +  S+S+   F   + +E++    ++    S  +  G     GT  LLN+ + ++ P
Sbjct: 582 KSWDSWSDSEDEFFECVSDTEEMKEDKEEAENRSRSKPEGRLQPHGTHTLLNTQEPLYIP 641

Query: 583 FTQNAPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGVFEDF 642
            TQ    MTED+ EE+ + +   G S +     A+++   L SDM +FKAANP     DF
Sbjct: 642 ITQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCTLLDF 701

Query: 643 IRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPAL 702
           +RW+SP D+ ++  +  +G                RG LS RM   GN+W + W  A A 
Sbjct: 702 VRWYSPRDYVEEQVTDADG------------RVEVRGELSARMKIPGNMWVEAWETARAT 761

Query: 703 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIV-CTAFRAAADTLNQTSYGGLKLMKT 761
           P   QK L D  +E EK+LHYL   +P +L   ++ C    A      + S   L  +++
Sbjct: 762 PARRQKRLFDDTKEAEKVLHYLALQKPSELTRHLLPCVLHAALLKIKEEESAEDLPSVRS 821

BLAST of MC03g0061 vs. ExPASy Swiss-Prot
Match: Q15042 (Rab3 GTPase-activating protein catalytic subunit OS=Homo sapiens OX=9606 GN=RAB3GAP1 PE=1 SV=3)

HSP 1 Score: 186.4 bits (472), Expect = 1.4e-45
Identity = 204/778 (26.22%), Postives = 322/778 (41.39%), Query Frame = 0

Query: 51  DWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPV 110
           D+    H L   +G++EF+VIAP + S  +L   + + LLS+V+IAL N     P FV +
Sbjct: 110 DFPPRAHCLVRWYGLREFVVIAPAAHSDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQI 169

Query: 111 HDPSRKAYIGIQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNL 170
           H   R+ Y+G +  G      FE   +  +VP +  HL GL ++F SK       +    
Sbjct: 170 HHKWRRMYVG-ECQGPGVRTDFEMVHL-RKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVS 229

Query: 171 FKVNFTMKLTFRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDP 230
             + FT  L            P  DA +         G  G +++    P+    + EDP
Sbjct: 230 IAIRFTYVLQDWQQYFWPQQPPDIDALVG--------GEVGGLEF-GKLPFG---ACEDP 289

Query: 231 VKGFELIASWSEKMVESSLEMAELENSSPHEAKKWILFPILSPN---------------- 290
           +    L  +W        ++     +  P +A  W +    + N                
Sbjct: 290 ISELHLATTWPHLTEGIIVDNDVYSDLDPIQAPHWSVRVRKAENPQCLLGDFVTEFFKIC 349

Query: 291 ----ITDSTMG---------NIVGFASQLYLLIDALDVSFQAQFIEDFV----------- 350
                TD  +G                 L  L +   V      + + V           
Sbjct: 350 RRKESTDEILGRSAFEEEGKETADITHALSKLTEPASVPIHKLSVSNMVHTAKKKIRKHR 409

Query: 351 SIENPGSDNLKSSTVLP---PPTVIDRVLK-EIFHEGKKSPSKGEN-RTSQAIKAAPLDS 410
            +E    +N   +T+L    P  V ++ L      +    PS+ E+       K+AP DS
Sbjct: 410 GVEESPLNNDVLNTILLFLFPDAVSEKPLDGTTSTDNNNPPSESEDYNLYNQFKSAPSDS 469

Query: 411 LFTR--FCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTCLVN 470
           L  +   CL ++ F +  ++ +A LW EFV E+R+ WE    +P +  S   DL  CL++
Sbjct: 470 LTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGL-ASGPPDLRCCLLH 529

Query: 471 QKLQMLAICIEKKHQSSE----EFQDCLGSQDPDSPLIGTRVKPTSSK---KELGHL--S 530
           QKLQML  CIE+K    E       D       D+   G ++ P + K   KE G +  S
Sbjct: 530 QKLQMLNCCIERKKARDEGKKTSASDVTNIYPGDAGKAGDQLVPDNLKETDKEKGEVGKS 589

Query: 531 SEDESESKANLFRYSTKSEDVISITDQK----SPDSMR----RGSAGMVGTMMLLNSYQS 590
            +  S+S+   F   + +E++     +      P  M      G     G + LL++ + 
Sbjct: 590 WDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLYQHGKLTLLHNGEP 649

Query: 591 MHAPFTQNAPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKDILSSDMSAFKAANPDGV 650
           ++ P TQ    MTED+ EE+ + +   G S +     A+++   L SDM +FKAANP   
Sbjct: 650 LYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCS 709

Query: 651 FEDFIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWND 710
            EDF+RW+SP D+ ++    ++G            N   +G LS RM    N+W + W  
Sbjct: 710 LEDFVRWYSPRDYIEEEVIDEKG------------NVVLKGELSARMKIPSNMWVEAWET 769

Query: 711 APALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIV-CTAFRAAADTLNQTSYGGLK 761
           A  +P   Q+ L D  RE EK+LHYL   +P  L   ++ C    A      + S   + 
Sbjct: 770 AKPIPARRQRRLFDDTREAEKVLHYLAIQKPADLARHLLPCVIHAAVLKVKEEESLENIS 829

BLAST of MC03g0061 vs. ExPASy Swiss-Prot
Match: Q642R9 (Rab3 GTPase-activating protein catalytic subunit OS=Xenopus laevis OX=8355 GN=rab3gap1 PE=2 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.4e-45
Identity = 205/795 (25.79%), Postives = 343/795 (43.14%), Query Frame = 0

Query: 43  REFLCKLVDWELSLHELQLCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSS 102
           ++ LC   D+    H L   +G++EF+VI+P +   VI +S + + LLS+V+IAL N   
Sbjct: 102 QDLLCTNNDFPPVAHCLVRWYGLREFVVISPGANDAVISES-KCNLLLSSVSIALGNTGC 161

Query: 103 FWPAFVPVHDPSRKAYIGIQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYS 162
             P FV VH   RK Y+G +  G      FE   +  +VP +  HL GL ++F SK    
Sbjct: 162 QVPLFVQVHQKWRKLYVG-ECRGPGVRTDFEMVHL-RKVPNQYTHLSGLLDIFKSKIGCP 221

Query: 163 SVDISMNLFKVNFTMKLTFRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWS 222
              +      + FT  L            P  DA +         G  G +++    P+ 
Sbjct: 222 LTALPPVNIAIRFTYVLQDWQQYFWPQQPPDIDALIG--------GEVGGLEF-GKLPFG 281

Query: 223 EWYSSEDPVKGFELIASW---SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDST 282
              + EDP+    L  +W   +E ++  +   ++L+   P +A +W     +     D+ 
Sbjct: 282 ---ACEDPISELHLATTWPCLTEGIIVDNDVYSDLD---PLQAPQW----SVRVRKADNP 341

Query: 283 MGNIVGFASQLYLLIDALDVSFQAQFIEDFVSIENPGSDNLKSSTVLPPPT--------- 342
              +  F S+ + L    + + +      F      G+D  ++ + L  P          
Sbjct: 342 QCMLGDFVSEFFRLCRRKESTDELLGKSAFEENGKEGADISQALSKLTEPAPVPIHKLSV 401

Query: 343 -------------------------VIDRVLKEIF--------HEGKKSPSKGENRTSQA 402
                                    V++ +L  +F         E K S S G N +SQ+
Sbjct: 402 TSMVHSARKKIRKHRGADESPLNNDVLNAILFFLFPDTKSLDGSEAKPSTSTG-NISSQS 461

Query: 403 ----------IKAAPLDSLFTR--FCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQP 462
                     +K+AP +SL  +   CL ++ F +  ++ +A LW EFV E+R+ WE    
Sbjct: 462 ESEDYNLYSQLKSAPSNSLTYKLALCLCMVNFYHGGVKGVAHLWQEFVLEMRYRWENNFL 521

Query: 463 LPRMPISSSIDLSTCLVNQKLQMLAICIEKKHQSSEE------FQDCLGSQDPDSPLIGT 522
           +P +  + S DL  CL++QKLQML  C+E+K    E       +     S +  +  + +
Sbjct: 522 IPGL-ANGSPDLKCCLLHQKLQMLNCCLERKKARDEGKKGNPLYSSSESSVNKTASDLLS 581

Query: 523 RVKPTSSKKELGHLSSEDESESKANLFRYSTKSEDV----------ISITDQKSPDSMRR 582
            V+   SK E+   S +  S+S+   F   + +E++               +++P     
Sbjct: 582 PVEADKSKYEVAK-SWDSWSDSEEEFFECHSDTEELKESGQESARKAKEETKENPSPKPE 641

Query: 583 GSAGMVGTMMLLNSYQSMHAPFTQNAPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKD 642
           G     G +MLLNS + ++ P TQ+   MT+D+ EE+ + +   G S +     A+++  
Sbjct: 642 GRLHQSGNLMLLNSGEPLYIPVTQDPAPMTDDLLEEQSEVLAKLGTSAEGAHLRARMQSA 701

Query: 643 ILSSDMSAFKAANPDGVFEDFIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHL 702
            L SDM +FKAANP    EDF+RW+SP D+ ++    D+G            N   +G L
Sbjct: 702 CLLSDMESFKAANPGCCLEDFVRWYSPRDYIEEEVMDDKG------------NKIFKGEL 761

Query: 703 SKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIV-CTA 761
           S RM    N+W + W  A  +P   Q+ L D  +E EK+LHYL   +P  L   ++ C  
Sbjct: 762 SARMKIPNNMWVEAWETAKPIPARRQRRLFDDTKEAEKVLHYLAVQKPADLTRHLLPCVI 821

BLAST of MC03g0061 vs. ExPASy Swiss-Prot
Match: P69735 (Rab3 GTPase-activating protein catalytic subunit (Fragments) OS=Rattus norvegicus OX=10116 GN=Rab3gap1 PE=1 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 9.9e-36
Identity = 128/435 (29.43%), Postives = 199/435 (45.75%), Query Frame = 0

Query: 350 GENRTSQAIKAAPLDSLFTR--FCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLP 409
           G+       K+AP DSL  +   CL ++ F +  ++ +A LW EFV E+R+ WE    +P
Sbjct: 233 GDYTLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIP 292

Query: 410 RMPISSSIDLSTCLVNQKLQMLAICIEKKHQSSE----EFQDCLGSQDP-DSPLIG---- 469
            +  S   DL  CL++QKLQML  CIE+K    E       D +    P D+   G    
Sbjct: 293 GL-ASGPPDLRCCLLHQKLQMLNCCIERKKARDEGKKTSPSDSMTKAYPADAGKAGGQLG 352

Query: 470 -TRVKPTSSKKELGHLSSEDESESKANLFRYSTKSEDV----ISITDQKSPDSMR----R 529
              ++ T  +K     S +  S+S+   F   + +ED+       T +  P  M      
Sbjct: 353 LDHLRDTEKEKGEAGKSWDSWSDSEEEFFECLSDAEDLRGNGQESTKKGGPKDMAPLKPE 412

Query: 530 GSAGMVGTMMLLNSYQSMHAPFTQNAPLMTEDMHEERLQAVEAFGDSFD---FSAQLEKD 589
           G     G + LL + + ++ P TQ    MTED+ EE+ + +   G S +     A+++  
Sbjct: 413 GRLHQHGKLTLLRNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSA 472

Query: 590 ILSSDMSAFKAANPDGVFEDFIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHL 649
            L SDM +FKAANP    EDF+RW+SP D+ ++  + ++G            N   +G L
Sbjct: 473 CLLSDMESFKAANPGCCLEDFVRWYSPRDYIEEEVTDEKG------------NVVLKGEL 532

Query: 650 SKRMSEHGNLWRQLWNDAPALPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIV-CTA 709
           S RM    N+W + W  A  +P   Q+ L D  RE EK+LHYL   +P  L   ++ C  
Sbjct: 533 SARMKIPSNMWVEAWETAKPVPARRQRRLFDDTREAEKVLHYLAMQKPADLARHLLPCVI 592

Query: 710 FRAAADTLNQTSYGGLKLMKTKMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFE 761
             A      + S   +  +K  + Q+ +  + VL+F         S     L  + +   
Sbjct: 593 HAAVLKVKEEESLENIPSVKKIIKQIIAHSSKVLRF--------PSPEDKKLEEIILQIT 646

BLAST of MC03g0061 vs. NCBI nr
Match: XP_022150875.1 (rab3 GTPase-activating protein catalytic subunit [Momordica charantia])

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 866/904 (95.80%), Postives = 868/904 (96.02%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L EKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHG      KLVDWELSLHELQ
Sbjct: 55  LLEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHG------KLVDWELSLHELQ 114

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG
Sbjct: 115 LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 174

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT
Sbjct: 175 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 234

Query: 181 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 240
           FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW
Sbjct: 235 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 294

Query: 241 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 300
           SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS
Sbjct: 295 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 354

Query: 301 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSPSKGENRTSQAIKA 360
           FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSPSKGENRTSQAIKA
Sbjct: 355 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSPSKGENRTSQAIKA 414

Query: 361 APLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTC 420
           APLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTC
Sbjct: 415 APLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTC 474

Query: 421 LVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSP------LIGTRVKPTSSKKEL---- 480
           LVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSP      L+        +  E     
Sbjct: 475 LVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPRDEEDSLVSEDFSIMQTPGETFDGK 534

Query: 481 --GHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMH 540
              HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMH
Sbjct: 535 CDSHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMH 594

Query: 541 APFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFI 600
           APFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFI
Sbjct: 595 APFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFI 654

Query: 601 RWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALP 660
           RWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALP
Sbjct: 655 RWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALP 714

Query: 661 VSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKM 720
           VSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKM
Sbjct: 715 VSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKM 774

Query: 721 DQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLS 780
           DQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLS
Sbjct: 775 DQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLS 834

Query: 781 EAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRVLS 840
           EAIFKDYYDFYLPRMGTGSSGNKFET     QVVRNHEREAVSSMFTPPTASQLWRRVLS
Sbjct: 835 EAIFKDYYDFYLPRMGTGSSGNKFETEFDKQQVVRNHEREAVSSMFTPPTASQLWRRVLS 894

Query: 841 MGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALSV 892
           MGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALSV
Sbjct: 895 MGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALSV 952

BLAST of MC03g0061 vs. NCBI nr
Match: XP_022949104.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucurbita moschata])

HSP 1 Score: 1559 bits (4036), Expect = 0.0
Identity = 790/906 (87.20%), Postives = 831/906 (91.72%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L +K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQ
Sbjct: 55  LLKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQ 114

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG
Sbjct: 115 LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 174

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DISMNLFKV+FTMKLT
Sbjct: 175 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLT 234

Query: 181 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 240
           F++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASW
Sbjct: 235 FKILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASW 294

Query: 241 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 300
           SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+S
Sbjct: 295 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMS 354

Query: 301 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAI 360
           FQAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  +KGE+RTSQAI
Sbjct: 355 FQAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAI 414

Query: 361 KAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS 420
           KAA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS
Sbjct: 415 KAAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLS 474

Query: 421 TCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG---- 480
           +CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G    
Sbjct: 475 SCLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFD 534

Query: 481 -----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQS 540
                HLS E E ESK NLF   TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQS
Sbjct: 535 GKCDSHLSPEVEFESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQS 594

Query: 541 MHAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 600
           MHAPFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED
Sbjct: 595 MHAPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 654

Query: 601 FIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPA 660
           FIRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPA
Sbjct: 655 FIRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPA 714

Query: 661 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKT 720
           LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKT
Sbjct: 715 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKT 774

Query: 721 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 780
           KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR
Sbjct: 775 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 834

Query: 781 LSEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRV 840
           LSEAIFKDYY+FYLPRMGTG S NKFET     Q+VR+HER A+SS+FTPPTASQ WR+V
Sbjct: 835 LSEAIFKDYYEFYLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKV 894

Query: 841 LSMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVAL 892
           LSMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVAL
Sbjct: 895 LSMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVAL 953

BLAST of MC03g0061 vs. NCBI nr
Match: XP_022949105.1 (rab3 GTPase-activating protein catalytic subunit isoform X2 [Cucurbita moschata])

HSP 1 Score: 1558 bits (4035), Expect = 0.0
Identity = 789/905 (87.18%), Postives = 831/905 (91.82%), Query Frame = 0

Query: 2   QEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQL 61
           ++K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQL
Sbjct: 3   KKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQL 62

Query: 62  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 121
           CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI
Sbjct: 63  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 122

Query: 122 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLTF 181
           QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DISMNLFKV+FTMKLTF
Sbjct: 123 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLTF 182

Query: 182 RVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASWS 241
           ++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASWS
Sbjct: 183 KILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWS 242

Query: 242 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVSF 301
           EKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+SF
Sbjct: 243 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSF 302

Query: 302 QAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAIK 361
           QAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  +KGE+RTSQAIK
Sbjct: 303 QAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAIK 362

Query: 362 AAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLST 421
           AA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+
Sbjct: 363 AAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSS 422

Query: 422 CLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG----- 481
           CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G     
Sbjct: 423 CLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDG 482

Query: 482 ----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSM 541
               HLS E E ESK NLF   TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQSM
Sbjct: 483 KCDSHLSPEVEFESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSM 542

Query: 542 HAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 601
           HAPFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF
Sbjct: 543 HAPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 602

Query: 602 IRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPAL 661
           IRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPAL
Sbjct: 603 IRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPAL 662

Query: 662 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTK 721
           PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKTK
Sbjct: 663 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTK 722

Query: 722 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 781
           MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL
Sbjct: 723 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 782

Query: 782 SEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRVL 841
           SEAIFKDYY+FYLPRMGTG S NKFET     Q+VR+HER A+SS+FTPPTASQ WR+VL
Sbjct: 783 SEAIFKDYYEFYLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKVL 842

Query: 842 SMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALS 892
           SMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVALS
Sbjct: 843 SMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALS 900

BLAST of MC03g0061 vs. NCBI nr
Match: XP_022998101.1 (rab3 GTPase-activating protein catalytic subunit isoform X1 [Cucurbita maxima])

HSP 1 Score: 1556 bits (4029), Expect = 0.0
Identity = 788/906 (86.98%), Postives = 832/906 (91.83%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L +K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQ
Sbjct: 55  LLKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQ 114

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG
Sbjct: 115 LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 174

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS++ISMNLFKV+FTMKLT
Sbjct: 175 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLT 234

Query: 181 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 240
           F++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASW
Sbjct: 235 FKILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASW 294

Query: 241 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 300
           SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+S
Sbjct: 295 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMS 354

Query: 301 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAI 360
           FQAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  SKGE+RTSQAI
Sbjct: 355 FQAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAI 414

Query: 361 KAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS 420
           KAA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS
Sbjct: 415 KAAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLS 474

Query: 421 TCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG---- 480
           +CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G    
Sbjct: 475 SCLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFD 534

Query: 481 -----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQS 540
                H S E E ESKANLF+  TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQS
Sbjct: 535 GKCDSHSSPEVEFESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQS 594

Query: 541 MHAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 600
           MH+PFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED
Sbjct: 595 MHSPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 654

Query: 601 FIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPA 660
           FIRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPA
Sbjct: 655 FIRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPA 714

Query: 661 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKT 720
           LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKT
Sbjct: 715 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKT 774

Query: 721 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 780
           KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR
Sbjct: 775 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 834

Query: 781 LSEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRV 840
           LSEAIFKDYY+FYLPRMGTG S NKF+T     Q+VR+HER A+SS+FTPPTASQ WR+V
Sbjct: 835 LSEAIFKDYYEFYLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKV 894

Query: 841 LSMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVAL 892
           LSMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVAL
Sbjct: 895 LSMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVAL 953

BLAST of MC03g0061 vs. NCBI nr
Match: XP_022998102.1 (rab3 GTPase-activating protein catalytic subunit isoform X2 [Cucurbita maxima])

HSP 1 Score: 1556 bits (4028), Expect = 0.0
Identity = 787/905 (86.96%), Postives = 832/905 (91.93%), Query Frame = 0

Query: 2   QEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQL 61
           ++K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQL
Sbjct: 3   KKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQL 62

Query: 62  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 121
           CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI
Sbjct: 63  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 122

Query: 122 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLTF 181
           QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS++ISMNLFKV+FTMKLTF
Sbjct: 123 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLTF 182

Query: 182 RVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASWS 241
           ++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASWS
Sbjct: 183 KILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWS 242

Query: 242 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVSF 301
           EKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+SF
Sbjct: 243 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSF 302

Query: 302 QAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAIK 361
           QAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  SKGE+RTSQAIK
Sbjct: 303 QAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAIK 362

Query: 362 AAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLST 421
           AA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+
Sbjct: 363 AAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSS 422

Query: 422 CLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG----- 481
           CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G     
Sbjct: 423 CLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDG 482

Query: 482 ----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSM 541
               H S E E ESKANLF+  TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQSM
Sbjct: 483 KCDSHSSPEVEFESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSM 542

Query: 542 HAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 601
           H+PFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF
Sbjct: 543 HSPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 602

Query: 602 IRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPAL 661
           IRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPAL
Sbjct: 603 IRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPAL 662

Query: 662 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTK 721
           PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKTK
Sbjct: 663 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTK 722

Query: 722 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 781
           MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL
Sbjct: 723 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 782

Query: 782 SEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRVL 841
           SEAIFKDYY+FYLPRMGTG S NKF+T     Q+VR+HER A+SS+FTPPTASQ WR+VL
Sbjct: 783 SEAIFKDYYEFYLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKVL 842

Query: 842 SMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALS 892
           SMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVALS
Sbjct: 843 SMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALS 900

BLAST of MC03g0061 vs. ExPASy TrEMBL
Match: A0A6J1D9P3 (Rab3 GTPase-activating protein catalytic subunit OS=Momordica charantia OX=3673 GN=LOC111018925 PE=3 SV=1)

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 866/904 (95.80%), Postives = 868/904 (96.02%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L EKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHG      KLVDWELSLHELQ
Sbjct: 55  LLEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHG------KLVDWELSLHELQ 114

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG
Sbjct: 115 LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 174

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT
Sbjct: 175 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 234

Query: 181 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 240
           FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW
Sbjct: 235 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 294

Query: 241 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 300
           SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS
Sbjct: 295 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 354

Query: 301 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSPSKGENRTSQAIKA 360
           FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSPSKGENRTSQAIKA
Sbjct: 355 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSPSKGENRTSQAIKA 414

Query: 361 APLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTC 420
           APLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTC
Sbjct: 415 APLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLSTC 474

Query: 421 LVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSP------LIGTRVKPTSSKKEL---- 480
           LVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSP      L+        +  E     
Sbjct: 475 LVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPRDEEDSLVSEDFSIMQTPGETFDGK 534

Query: 481 --GHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMH 540
              HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMH
Sbjct: 535 CDSHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMH 594

Query: 541 APFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFI 600
           APFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFI
Sbjct: 595 APFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFI 654

Query: 601 RWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALP 660
           RWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALP
Sbjct: 655 RWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALP 714

Query: 661 VSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKM 720
           VSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKM
Sbjct: 715 VSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKM 774

Query: 721 DQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLS 780
           DQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLS
Sbjct: 775 DQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRLS 834

Query: 781 EAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRVLS 840
           EAIFKDYYDFYLPRMGTGSSGNKFET     QVVRNHEREAVSSMFTPPTASQLWRRVLS
Sbjct: 835 EAIFKDYYDFYLPRMGTGSSGNKFETEFDKQQVVRNHEREAVSSMFTPPTASQLWRRVLS 894

Query: 841 MGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALSV 892
           MGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALSV
Sbjct: 895 MGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALSV 952

BLAST of MC03g0061 vs. ExPASy TrEMBL
Match: A0A6J1GBV8 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452560 PE=3 SV=1)

HSP 1 Score: 1559 bits (4036), Expect = 0.0
Identity = 790/906 (87.20%), Postives = 831/906 (91.72%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L +K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQ
Sbjct: 55  LLKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQ 114

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG
Sbjct: 115 LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 174

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DISMNLFKV+FTMKLT
Sbjct: 175 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLT 234

Query: 181 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 240
           F++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASW
Sbjct: 235 FKILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASW 294

Query: 241 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 300
           SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+S
Sbjct: 295 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMS 354

Query: 301 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAI 360
           FQAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  +KGE+RTSQAI
Sbjct: 355 FQAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAI 414

Query: 361 KAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS 420
           KAA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS
Sbjct: 415 KAAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLS 474

Query: 421 TCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG---- 480
           +CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G    
Sbjct: 475 SCLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFD 534

Query: 481 -----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQS 540
                HLS E E ESK NLF   TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQS
Sbjct: 535 GKCDSHLSPEVEFESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQS 594

Query: 541 MHAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 600
           MHAPFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED
Sbjct: 595 MHAPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 654

Query: 601 FIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPA 660
           FIRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPA
Sbjct: 655 FIRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPA 714

Query: 661 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKT 720
           LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKT
Sbjct: 715 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKT 774

Query: 721 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 780
           KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR
Sbjct: 775 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 834

Query: 781 LSEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRV 840
           LSEAIFKDYY+FYLPRMGTG S NKFET     Q+VR+HER A+SS+FTPPTASQ WR+V
Sbjct: 835 LSEAIFKDYYEFYLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKV 894

Query: 841 LSMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVAL 892
           LSMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVAL
Sbjct: 895 LSMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVAL 953

BLAST of MC03g0061 vs. ExPASy TrEMBL
Match: A0A6J1GBV2 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452560 PE=3 SV=1)

HSP 1 Score: 1558 bits (4035), Expect = 0.0
Identity = 789/905 (87.18%), Postives = 831/905 (91.82%), Query Frame = 0

Query: 2   QEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQL 61
           ++K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQL
Sbjct: 3   KKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQL 62

Query: 62  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 121
           CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI
Sbjct: 63  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 122

Query: 122 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLTF 181
           QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS+DISMNLFKV+FTMKLTF
Sbjct: 123 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMDISMNLFKVHFTMKLTF 182

Query: 182 RVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASWS 241
           ++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASWS
Sbjct: 183 KILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWS 242

Query: 242 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVSF 301
           EKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+SF
Sbjct: 243 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSF 302

Query: 302 QAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAIK 361
           QAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  +KGE+RTSQAIK
Sbjct: 303 QAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFAKGEHRTSQAIK 362

Query: 362 AAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLST 421
           AA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+
Sbjct: 363 AAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSS 422

Query: 422 CLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG----- 481
           CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G     
Sbjct: 423 CLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDG 482

Query: 482 ----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSM 541
               HLS E E ESK NLF   TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQSM
Sbjct: 483 KCDSHLSPEVEFESKGNLFEERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSM 542

Query: 542 HAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 601
           HAPFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF
Sbjct: 543 HAPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 602

Query: 602 IRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPAL 661
           IRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPAL
Sbjct: 603 IRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPAL 662

Query: 662 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTK 721
           PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKTK
Sbjct: 663 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTK 722

Query: 722 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 781
           MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL
Sbjct: 723 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 782

Query: 782 SEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRVL 841
           SEAIFKDYY+FYLPRMGTG S NKFET     Q+VR+HER A+SS+FTPPTASQ WR+VL
Sbjct: 783 SEAIFKDYYEFYLPRMGTGMSSNKFETEFNKKQLVRSHERGAISSLFTPPTASQSWRKVL 842

Query: 842 SMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALS 892
           SMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVALS
Sbjct: 843 SMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALS 900

BLAST of MC03g0061 vs. ExPASy TrEMBL
Match: A0A6J1K9B0 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111492852 PE=3 SV=1)

HSP 1 Score: 1556 bits (4029), Expect = 0.0
Identity = 788/906 (86.98%), Postives = 832/906 (91.83%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L +K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQ
Sbjct: 55  LLKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQ 114

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG
Sbjct: 115 LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 174

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS++ISMNLFKV+FTMKLT
Sbjct: 175 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLT 234

Query: 181 FRVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASW 240
           F++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASW
Sbjct: 235 FKILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASW 294

Query: 241 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVS 300
           SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+S
Sbjct: 295 SEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMS 354

Query: 301 FQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAI 360
           FQAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  SKGE+RTSQAI
Sbjct: 355 FQAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAI 414

Query: 361 KAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLS 420
           KAA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS
Sbjct: 415 KAAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLS 474

Query: 421 TCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG---- 480
           +CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G    
Sbjct: 475 SCLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFD 534

Query: 481 -----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQS 540
                H S E E ESKANLF+  TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQS
Sbjct: 535 GKCDSHSSPEVEFESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQS 594

Query: 541 MHAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 600
           MH+PFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED
Sbjct: 595 MHSPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFED 654

Query: 601 FIRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPA 660
           FIRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPA
Sbjct: 655 FIRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPA 714

Query: 661 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKT 720
           LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKT
Sbjct: 715 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKT 774

Query: 721 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 780
           KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR
Sbjct: 775 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 834

Query: 781 LSEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRV 840
           LSEAIFKDYY+FYLPRMGTG S NKF+T     Q+VR+HER A+SS+FTPPTASQ WR+V
Sbjct: 835 LSEAIFKDYYEFYLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKV 894

Query: 841 LSMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVAL 892
           LSMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVAL
Sbjct: 895 LSMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVAL 953

BLAST of MC03g0061 vs. ExPASy TrEMBL
Match: A0A6J1K6Y4 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111492852 PE=3 SV=1)

HSP 1 Score: 1556 bits (4028), Expect = 0.0
Identity = 787/905 (86.96%), Postives = 832/905 (91.93%), Query Frame = 0

Query: 2   QEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQL 61
           ++K AT LEISTNLYKVK DLKCDNKNYSMEYYFGNSNHG      K+VDWELSLHELQL
Sbjct: 3   KKKDATPLEISTNLYKVKSDLKCDNKNYSMEYYFGNSNHG------KVVDWELSLHELQL 62

Query: 62  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 121
           CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI
Sbjct: 63  CFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIGI 122

Query: 122 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLTF 181
           QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSS++ISMNLFKV+FTMKLTF
Sbjct: 123 QNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSMEISMNLFKVHFTMKLTF 182

Query: 182 RVLLSDDDNIPRADAEMTESEHESAVGTRGKVQWDDDCPWSEWYSSEDPVKGFELIASWS 241
           ++LLSDDD+I R DAEMTES +ESAVGT GK QWDDDCPWSEWYSSEDPVKGFELIASWS
Sbjct: 183 KILLSDDDDIRRDDAEMTESVYESAVGTSGKGQWDDDCPWSEWYSSEDPVKGFELIASWS 242

Query: 242 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDALDVSF 301
           EKMVESSLEMAELENSSPHEAKKWILFPILSPNITD+TMGN VGF+SQLYLLIDALD+SF
Sbjct: 243 EKMVESSLEMAELENSSPHEAKKWILFPILSPNITDTTMGNRVGFSSQLYLLIDALDMSF 302

Query: 302 QAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRTSQAIK 361
           QAQF+EDFVS+ENPGSDN KSST +PPPTVIDRVLKE+FHEGKKSP  SKGE+RTSQAIK
Sbjct: 303 QAQFMEDFVSVENPGSDNFKSSTAVPPPTVIDRVLKELFHEGKKSPYFSKGEHRTSQAIK 362

Query: 362 AAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSSIDLST 421
           AA LDSLF +FCL VLWFGNCNIRAIATLWVEFVREVRWCW+EVQPLPRMPISSSIDLS+
Sbjct: 363 AAALDSLFAQFCLHVLWFGNCNIRAIATLWVEFVREVRWCWDEVQPLPRMPISSSIDLSS 422

Query: 422 CLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLI---GTRVKPTSSKKELG----- 481
           CL+NQKLQMLAICIE+KHQS EEFQDCL SQDP+SP     G   +  S+ +  G     
Sbjct: 423 CLINQKLQMLAICIERKHQSIEEFQDCLESQDPESPRAEEDGPVSEDLSTMQTPGENFDG 482

Query: 482 ----HLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSM 541
               H S E E ESKANLF+  TKSEDVIS TDQKS DS RRGSAG+VGTMMLLNSYQSM
Sbjct: 483 KCDSHSSPEVEFESKANLFKERTKSEDVISFTDQKSTDSTRRGSAGIVGTMMLLNSYQSM 542

Query: 542 HAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 601
           H+PFTQ+APLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF
Sbjct: 543 HSPFTQDAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 602

Query: 602 IRWHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPAL 661
           IRWHSP DWE D D KDEGLPNGSATETSK+NWPPRGHLSKRMSEHGNLWRQLWNDAPAL
Sbjct: 603 IRWHSPRDWE-DSDPKDEGLPNGSATETSKDNWPPRGHLSKRMSEHGNLWRQLWNDAPAL 662

Query: 662 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTK 721
           PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQ+VCTAF+A ADTL+QTSYGGLKLMKTK
Sbjct: 663 PVSEQKSLLDPNREGEKILHYLETLRPHQLLEQMVCTAFKAGADTLSQTSYGGLKLMKTK 722

Query: 722 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 781
           MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL
Sbjct: 723 MDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPRL 782

Query: 782 SEAIFKDYYDFYLPRMGTGSSGNKFETVTISFQVVRNHEREAVSSMFTPPTASQLWRRVL 841
           SEAIFKDYY+FYLPRMGTG S NKF+T     Q+VR+HER A+SS+FTPPTASQ WR+VL
Sbjct: 783 SEAIFKDYYEFYLPRMGTGMSSNKFDTEFNKKQLVRSHERGAISSLFTPPTASQSWRKVL 842

Query: 842 SMGNLLNGHEPILREIVFSLRDRVNENHYADSTPKAQGEVIETHRMYISGTSNDLRVALS 892
           SMGNL NGHEPILREI+FSLRDRV+ NHYADSTP A+GE IETHRMYISGTSNDLRVALS
Sbjct: 843 SMGNLFNGHEPILREIIFSLRDRVSGNHYADSTPTARGEAIETHRMYISGTSNDLRVALS 900

BLAST of MC03g0061 vs. TAIR 10
Match: AT5G58510.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G55060.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 563/915 (61.53%), Postives = 702/915 (76.72%), Query Frame = 0

Query: 1   LQEKGATQLEISTNLYKVKYDLKCDNKNYSMEYYFGNSNHGTREFLCKLVDWELSLHELQ 60
           L EKGA  +E S NL+ VK++LK   K+Y ME+YF   N+G+++    + +W  + H+LQ
Sbjct: 54  LVEKGAVAVEDSKNLFTVKHELKNVAKSYCMEFYFQIDNNGSQQ--AGIGNWNSNSHDLQ 113

Query: 61  LCFGVQEFLVIAPQSISGVILDSPEASKLLSAVAIALTNCSSFWPAFVPVHDPSRKAYIG 120
           LCFGV++FL+IAPQS SGV+LD+PE+SKLLSAVAIAL+NC S WPAFVPVHDPSRKAYIG
Sbjct: 114 LCFGVKDFLLIAPQSASGVLLDTPESSKLLSAVAIALSNCGSLWPAFVPVHDPSRKAYIG 173

Query: 121 IQNMGTTFTRRFEADRIGTQVPIKLMHLEGLYELFVSKFAYSSVDISMNLFKVNFTMKLT 180
           IQNMGT FTRRFEADR+G+QVP+KLMHLEGLYELFVSKF YS VD SM+ F+V+F M+LT
Sbjct: 174 IQNMGTVFTRRFEADRVGSQVPVKLMHLEGLYELFVSKFVYSGVDFSMHTFRVHFMMRLT 233

Query: 181 FRVLLSD--DDNIPRADAEMTESEHESAVG--TRGKVQWDDDCPWSEWYSSEDPVKGFEL 240
           ++    D  D+ I   +    +S+     G  +R KV WDDDCPWSEWYS+EDP++GFEL
Sbjct: 234 YQTFPYDEEDEEIDTDEVMGDKSDTAEHYGSESRNKVLWDDDCPWSEWYSAEDPLRGFEL 293

Query: 241 IASWSEKMVESSLEMAELENSSPHEAKKWILFPILSPNITDSTMGNIVGFASQLYLLIDA 300
           + +W+++ VES+LEMAELEN+SPH+A+KWIL PILSP + D + G  + FASQL  L++A
Sbjct: 294 VVTWADRTVESTLEMAELENASPHDAEKWILHPILSPYLGDPSHGKRIDFASQLLCLVEA 353

Query: 301 LDVSFQAQFIEDFVSIENPGSDNLKSSTVLPPPTVIDRVLKEIFHEGKKSP--SKGENRT 360
           LD SF AQF+EDFVS+ENP S+NLK+S V+PPP+V+DRV+K++F EG K P  +KGE+R 
Sbjct: 354 LDSSFSAQFMEDFVSVENPSSENLKTSVVIPPPSVLDRVIKDLFREGSKLPDFTKGEHRL 413

Query: 361 SQAIKAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRMPISSS 420
           S+A+KAAPL+SLFT+FCL  LWFGNCNIRAIA LW+EFVREVRWCWEE QPLP+MPI  S
Sbjct: 414 SRALKAAPLESLFTQFCLHSLWFGNCNIRAIAFLWIEFVREVRWCWEETQPLPKMPIDGS 473

Query: 421 IDLSTCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLIG-------TRVKPTSSK 480
           IDLS+CL+NQKL +LAICIEKK + +EEF DC+GS D     +         + + TSS+
Sbjct: 474 IDLSSCLINQKLHLLAICIEKKREMNEEFLDCIGSDDSSDASVSMEEHHKVDKRRNTSSE 533

Query: 481 KELGHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSM 540
           +EL        +E  +   R+  K+E   S+ +Q   D++RRGSAG VGTMMLL S Q +
Sbjct: 534 EELRRKRDSSIAEDTSKQLRFERKTERTNSV-NQSPTDAIRRGSAGPVGTMMLLKSRQQL 593

Query: 541 HAPFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDF 600
           HAPFTQ+ PLMTEDMHEERLQAVEAFGDS +   QLEKDIL SDMSAFKAANPD VFEDF
Sbjct: 594 HAPFTQDPPLMTEDMHEERLQAVEAFGDSLNVPGQLEKDILLSDMSAFKAANPDAVFEDF 653

Query: 601 IRWHSPGDWEK-DPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPA 660
           IRWHSPGDWE  +P + +     G +TE SK+ WPPRG LS+RMS+ GNLWR+ WNDAPA
Sbjct: 654 IRWHSPGDWESFEPKTTEPSA--GPSTEGSKDEWPPRGRLSQRMSDQGNLWRKSWNDAPA 713

Query: 661 LPVSEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKT 720
           LP  +QK LLDPNREGEKI+HYLET+RPHQLLEQ+VCTAFR +ADTLNQT+ G ++ M +
Sbjct: 714 LPADDQKPLLDPNREGEKIVHYLETVRPHQLLEQMVCTAFRGSADTLNQTNVGNMRQMTS 773

Query: 721 KMDQLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQAPR 780
           K++QLY  M   L  LQ N L  +++ + DL+RLC+VFE+VEKL+A+AAS+HRK L A R
Sbjct: 774 KLEQLYLIMKFTLGALQRNNLPDKAKTVKDLKRLCMVFENVEKLVAVAASIHRKFLDASR 833

Query: 781 LSEAIFKDYYDFYLPRMGTGSSG--NKFET-VTISFQVVRNHEREAVSSMFTPPTASQLW 840
           L++ IF D+Y  Y P MG  ++   NK  T + +S Q V   ER+ VS++F+PP+A+Q W
Sbjct: 834 LAQVIFSDFYGVYAPTMGMSANDEENKSRTEMEVSRQEVSLRERQVVSNLFSPPSANQSW 893

Query: 841 RRVLSMGNLLNGHEPILREIVFSLRDRVNEN-HYA-----DSTPKAQGEVIETHRMYISG 893
           R+VLSMGNLLNGHEPILREI+FS  D VN   HYA      +T   +GE IETHRMY+SG
Sbjct: 894 RKVLSMGNLLNGHEPILREIIFSTGDDVNNGIHYAAAADVAATSDRKGEEIETHRMYVSG 953

BLAST of MC03g0061 vs. TAIR 10
Match: AT5G55060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1); Has 223 Blast hits to 218 proteins in 80 species: Archae - 0; Bacteria - 2; Metazoa - 117; Fungi - 6; Plants - 65; Viruses - 0; Other Eukaryotes - 33 (source: NCBI BLink). )

HSP 1 Score: 147.5 bits (371), Expect = 5.1e-35
Identity = 123/414 (29.71%), Postives = 190/414 (45.89%), Query Frame = 0

Query: 352 NRTSQA--IKAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRM 411
           NR+S A  + AAP  S   +    +  F     R +A  W   V E+R  W E + +P +
Sbjct: 230 NRSSFAKDLHAAPPSSFVVKLAEVIGSF--TTPRRMALFWCRVVEELRRFWNEEKHIPWI 289

Query: 412 PISSSIDLSTCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLIGTRVKPTSSKKE 471
           P+ ++ DL +CL++Q LQ++  C+ +K ++              S  +   ++  SS  E
Sbjct: 290 PLDNNPDLKSCLLHQWLQVINCCLARKARNVAA-----------SEALDAVMRQASSANE 349

Query: 472 LGHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMHA 531
                  D SES  +L    + S ++I          +R G    V  + +L + + +++
Sbjct: 350 -----KSDVSESMGSLLYAKSNSGELI----------LRLGVYNQVENLTMLETGEPVYS 409

Query: 532 PFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIR 591
           P TQ  PL+TED+  E  + V   G      +Q     L SDM AFKAANP  + EDF+R
Sbjct: 410 PITQEGPLLTEDLIRETEELVLRTGSMGAGCSQ-----LLSDMQAFKAANPGCILEDFVR 469

Query: 592 WHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALPV 651
           WHSP DW +          NG+   +  ++ P RG LS RM + GNLWR+LW  A  LP 
Sbjct: 470 WHSPPDWTE----------NGNM--SGDDSSPVRGQLSTRMQKEGNLWRELWETAKPLPA 529

Query: 652 SEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKMD 711
            +Q  L D +   E IL+YLE +   +L EQ+  +        +         L K    
Sbjct: 530 VKQAPLFDEDLAVEGILNYLEDIPAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKL--- 588

Query: 712 QLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQ 764
             +     V+   QG    A ++ +DD   LC V+E VE ++     + R + Q
Sbjct: 590 -FFECKDYVVAICQG---GAWTDKLDD---LCQVYETVETMLLRPEEVLRSMKQ 588

BLAST of MC03g0061 vs. TAIR 10
Match: AT5G55060.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1). )

HSP 1 Score: 147.5 bits (371), Expect = 5.1e-35
Identity = 123/414 (29.71%), Postives = 190/414 (45.89%), Query Frame = 0

Query: 352 NRTSQA--IKAAPLDSLFTRFCLQVLWFGNCNIRAIATLWVEFVREVRWCWEEVQPLPRM 411
           NR+S A  + AAP  S   +    +  F     R +A  W   V E+R  W E + +P +
Sbjct: 137 NRSSFAKDLHAAPPSSFVVKLAEVIGSF--TTPRRMALFWCRVVEELRRFWNEEKHIPWI 196

Query: 412 PISSSIDLSTCLVNQKLQMLAICIEKKHQSSEEFQDCLGSQDPDSPLIGTRVKPTSSKKE 471
           P+ ++ DL +CL++Q LQ++  C+ +K ++              S  +   ++  SS  E
Sbjct: 197 PLDNNPDLKSCLLHQWLQVINCCLARKARNVAA-----------SEALDAVMRQASSANE 256

Query: 472 LGHLSSEDESESKANLFRYSTKSEDVISITDQKSPDSMRRGSAGMVGTMMLLNSYQSMHA 531
                  D SES  +L    + S ++I          +R G    V  + +L + + +++
Sbjct: 257 -----KSDVSESMGSLLYAKSNSGELI----------LRLGVYNQVENLTMLETGEPVYS 316

Query: 532 PFTQNAPLMTEDMHEERLQAVEAFGDSFDFSAQLEKDILSSDMSAFKAANPDGVFEDFIR 591
           P TQ  PL+TED+  E  + V   G      +Q     L SDM AFKAANP  + EDF+R
Sbjct: 317 PITQEGPLLTEDLIRETEELVLRTGSMGAGCSQ-----LLSDMQAFKAANPGCILEDFVR 376

Query: 592 WHSPGDWEKDPDSKDEGLPNGSATETSKENWPPRGHLSKRMSEHGNLWRQLWNDAPALPV 651
           WHSP DW +          NG+   +  ++ P RG LS RM + GNLWR+LW  A  LP 
Sbjct: 377 WHSPPDWTE----------NGNM--SGDDSSPVRGQLSTRMQKEGNLWRELWETAKPLPA 436

Query: 652 SEQKSLLDPNREGEKILHYLETLRPHQLLEQIVCTAFRAAADTLNQTSYGGLKLMKTKMD 711
            +Q  L D +   E IL+YLE +   +L EQ+  +        +         L K    
Sbjct: 437 VKQAPLFDEDLAVEGILNYLEDIPAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKL--- 495

Query: 712 QLYSTMASVLKFLQGNRLSAESEVIDDLRRLCVVFEHVEKLMALAASLHRKLLQ 764
             +     V+   QG    A ++ +DD   LC V+E VE ++     + R + Q
Sbjct: 497 -FFECKDYVVAICQG---GAWTDKLDD---LCQVYETVETMLLRPEEVLRSMKQ 495