MC03g0026 (gene) Bitter gourd (Dali-11) v1

BLAST of MC03g0026 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 8.8e-74
Identity = 197/532 (37.03%), Postives = 298/532 (56.02%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           +W  LVA IW+Q+  GTNF+F +YSS LK  L ISQ++LN LA ASD GK FG+ SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
            + PL +VL   + +G +GYG+Q+L +T+ +  +  Y L+FL  LLAG SICW NT C++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNII--TLPYSLVFLCCLLAGLSICWFNTACFI 162

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPSKRAETFLLLNSAVPLGVCVAA- 180
           + IR+F  N  LA+ ++ S+ G+SA +Y+    ++ PS  +  +LLLNS VPL V  AA 
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSS-SNLYLLLNSLVPLVVSFAAL 222

Query: 181 -----SPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGI 240
                 P        +  R+    F  + V+ + T    +++S  + S  L+ +  +V +
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLL 282

Query: 241 VVLLLLPLMVAAAE----------------KVKESVVERKERR--VYDFTGDGDLERVRP 300
           V  L  PL+V A +                 V  ++ E K ++  V   TG   +   + 
Sbjct: 283 VFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKE 342

Query: 301 KNEVKSGDGAMEEIGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSS 360
            N V+ GD    E     ++ R+ FWLY+  YF G T+GL + NNLGQIA+S    N ++
Sbjct: 343 GNTVRLGD----EHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQS-LGQNSTT 402

Query: 361 LVSLSSAFGFFGRLLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSN--A 420
           LV++ S+F FFGRLL +  D F  + +R       G  A  ++     FFLL   S+   
Sbjct: 403 LVTIYSSFSFFGRLLSAAPD-FMHKRFRL---TRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 421 SLCVSTAIIAGCTGAITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYH 480
           +L  +TA+I   +G I + +VS T+ LFG  +  VNHN+++ NIPIGS LYGY+AA +Y 
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE 522

Query: 481 SHPS-------AAAGKCMGVECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 500
           ++ S       + +  C+G +CY  TF+ WGCL   G + +L L++RT+  Y
Sbjct: 523 ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MC03g0026 vs. NCBI nr
Match: XP_022970645.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 695 bits (1793), Expect = 6.53e-247
Identity = 374/506 (73.91%), Postives = 421/506 (83.20%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLK  LSISQ QLNNLAFASDAGKLF  +SG+AA
Sbjct: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLW VL IGS+LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCY+
Sbjct: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHS-PSYCLIFFLTVLAGNSICWINTVCYI 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPS---KRAETFLLLNSAVPLGVCV 180
           VAIRNF  N Q+AVGISTSYQGLSAK+YTDIV  +F     K AETFLL NS  PLGVCV
Sbjct: 127 VAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVCV 186

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
             SP+AR+VE VEE  NLE GF  +FVITIATGVFAVVTS+GSISR LSPLS LVGI+V 
Sbjct: 187 LVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGILVF 246

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDGDLERVRPKNEVK----SGDGAMEEIGAM 300
           LLLP++V   E VK+SV E KERRVY  T + D  R+RP+NE K     GD AMEEIGAM
Sbjct: 247 LLLPVVVPVVESVKKSVREGKERRVYHSTMEEDT-RIRPENEAKLNRDGGDEAMEEIGAM 306

Query: 301 EMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRLLPS 360
           EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAESR S +VSSLVSLSS+FGFFGRL+PS
Sbjct: 307 EMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGRLMPS 366

Query: 361 LLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTGAITSI 420
           LLDHF SR  R    +  G + ALM  MCGGFFLL  P++  LCVSTA+IA CTGAITSI
Sbjct: 367 LLDHFLSR--RKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAITSI 426

Query: 421 SVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGKCMGVECYRTT 480
           SVSTT++LFGA NFS+NHN+VVANIP+GSFL+GYMAA+LY    +  AGKC+GVECYRTT
Sbjct: 427 SVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYRTT 486

Query: 481 FLIWGCLCSFGTLLALLLFLRTRSFY 499
           F+IWGCLCSFGTLLALLLF RTRSFY
Sbjct: 487 FMIWGCLCSFGTLLALLLFARTRSFY 508

BLAST of MC03g0026 vs. NCBI nr
Match: XP_038895111.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Benincasa hispida])

HSP 1 Score: 684 bits (1766), Expect = 9.26e-243
Identity = 373/510 (73.14%), Postives = 425/510 (83.33%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSLV IIWLQSINGTNFNFPSYSSQLKQQLS+SQLQLNNLAFASDAGKLF   SG+AA
Sbjct: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLWLVL IG++LGL+GYG+Q+LF+T+Q+ S PSY +IFLL++LAGNSICWINTVCYM
Sbjct: 68  NYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQS-PSYCVIFLLTVLAGNSICWINTVCYM 127

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFP---SKRAETFLLLNSAVPLGVCV 180
           VAI NF  N Q+AVGISTSYQGLSAK+YT+IV S+F    SK  ETFLLLNS +P+GVCV
Sbjct: 128 VAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVCV 187

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
            ASPLAR+++ VEE   LE GF  +FVITIATG+FA VTS+GSISR LSPLS LVGIVV 
Sbjct: 188 LASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLVGIVVF 247

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDG--DLERVRPKNEVK---SGDG--AMEEI 300
           L+LPL+VA  EKVKES+ E KERRVY FT +   D+ERVR +N+ K    GD   AMEEI
Sbjct: 248 LVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDSEEAMEEI 307

Query: 301 GAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRL 360
           GA EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR S++VSSLVSLSS+FGFFGRL
Sbjct: 308 GAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFFGRL 367

Query: 361 LPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTGAI 420
           +PS+LD+F SR  +    +  G M  LM  +CGGFFLL  P++ SLCVSTAIIA CTGAI
Sbjct: 368 MPSILDYFLSR--KKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIAVCTGAI 427

Query: 421 TSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSA-AAGKCMGVEC 480
           TSISVSTT++LFGA NFS+NHNL+VANIP GSFL+GYMAAFLYH        GKC+GVEC
Sbjct: 428 TSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAGGDGTGKCIGVEC 487

Query: 481 YRTTFLIWGCLCSFGTLLALLLFLRTRSFY 499
           YRTTF IWGC  SFGTLLALLLF RT++FY
Sbjct: 488 YRTTFFIWGCFSSFGTLLALLLFARTKTFY 514

BLAST of MC03g0026 vs. NCBI nr
Match: KAG6583327.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019101.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681 bits (1756), Expect = 2.85e-241
Identity = 370/507 (72.98%), Postives = 416/507 (82.05%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLK  LSISQ QLNNLAFASDAGKLF  +SG+AA
Sbjct: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLW +L IGS LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCY+
Sbjct: 67  NYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHS-PSYCLIFFLTVLAGNSICWINTVCYI 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPS---KRAETFLLLNSAVPLGVCV 180
           VAIRNF  N Q+AVGISTSYQGLSAK+YTDIV  +F     K AETFLL NS  PLGVCV
Sbjct: 127 VAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVCV 186

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
              P+AR+VE VEE  NLE GF  +FVITIATGVFAVVTS+GSISR LSPLS LVGI+V 
Sbjct: 187 LVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGILVF 246

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDGDLERVRPKNEVK----SGDGAMEEIGAM 300
           LLLP++V   E VK+SV E KERRVY  T + D  R+RP+NE K     GD AMEEIGAM
Sbjct: 247 LLLPVVVPVVESVKKSVREGKERRVYHSTMEEDT-RIRPENEAKLNRDGGDEAMEEIGAM 306

Query: 301 EMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRLLPS 360
           EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAESR S + SSLVSLSS+FGFFGRL+PS
Sbjct: 307 EMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESASSLVSLSSSFGFFGRLMPS 366

Query: 361 LLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFF-LLWWPSNASLCVSTAIIAGCTGAITS 420
           LLDHF SR  R    +  G + ALM  MCGGFF LL  P++  LCVSTA+IA CTGAITS
Sbjct: 367 LLDHFLSR--RKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAITS 426

Query: 421 ISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGKCMGVECYRT 480
           ISVSTT++LFGA NFS+NHN+VVANIP GSFL+GYMAA+LY       AGKC+GVECYRT
Sbjct: 427 ISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQARGDAGKCIGVECYRT 486

Query: 481 TFLIWGCLCSFGTLLALLLFLRTRSFY 499
           +F+IWGCLCSFGTLLALLLF RTRSFY
Sbjct: 487 SFMIWGCLCSFGTLLALLLFARTRSFY 509

BLAST of MC03g0026 vs. NCBI nr
Match: XP_023519273.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 679 bits (1752), Expect = 1.16e-240
Identity = 370/507 (72.98%), Postives = 414/507 (81.66%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLK  LSISQ QLNNLAFASDAGKLF  +SG+AA
Sbjct: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLW +L IGS LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCY+
Sbjct: 67  NYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHS-PSYCLIFFLTVLAGNSICWINTVCYI 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPS---KRAETFLLLNSAVPLGVCV 180
           VAIRNF  N Q+AVGISTSYQGLSAK+YTDIV  +F     K AETFLL NS  PLGVCV
Sbjct: 127 VAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFTKRSDKTAETFLLSNSVFPLGVCV 186

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
              P+AR+VE VEE  NL  GF  +FVITIATGVFAVVTS+GSISR LSPLS LVGI+V 
Sbjct: 187 LVCPMARIVELVEEKGNLGVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGILVF 246

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDGDLERVRPKNEVK----SGDGAMEEIGAM 300
           LLLP++V   E VK+SV E KERRVY  T + D  R+RP+NE K     GD AMEEIGA 
Sbjct: 247 LLLPVVVPVVESVKKSVREGKERRVYHSTMEEDT-RIRPENEAKLNRDGGDDAMEEIGAR 306

Query: 301 EMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRLLPS 360
           EMV R+NFWLY GVYFFGATLGLAFLNNLGQIAESR S +VSSLVSLSS+FGFFGRL+PS
Sbjct: 307 EMVTRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGRLMPS 366

Query: 361 LLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFF-LLWWPSNASLCVSTAIIAGCTGAITS 420
           LLDHF SR  R    +  G M  LM  MCGGFF LL  P++  LCVSTA+IA CTGAITS
Sbjct: 367 LLDHFLSR--RKMMKSKPGWMVGLMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAITS 426

Query: 421 ISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGKCMGVECYRT 480
           ISVSTT++LFGA NFS+NHN+VVANIP GSFL+GYMAA+LY    +  AGKC+GVECYRT
Sbjct: 427 ISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGGAGKCIGVECYRT 486

Query: 481 TFLIWGCLCSFGTLLALLLFLRTRSFY 499
           TF+IWGCLCSFGTLLALLLF RTRSFY
Sbjct: 487 TFMIWGCLCSFGTLLALLLFARTRSFY 509

BLAST of MC03g0026 vs. NCBI nr
Match: XP_008457307.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo])

HSP 1 Score: 676 bits (1745), Expect = 1.92e-239
Identity = 378/513 (73.68%), Postives = 422/513 (82.26%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLKQQLS+SQLQLNNLAFASDAGKLF   SG+AA
Sbjct: 9   QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLWLVL IGS+LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCYM
Sbjct: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHS-PSYWLIFFLTVLAGNSICWINTVCYM 128

Query: 121 VAIRNF-SFNPQLAVGISTSYQGLSAKVYTDIVESMFP----SKRAETFLLLNSAVPLGV 180
           VAI NF S N Q+AVGISTSYQGLSAKVYTDIV S+F     SK AETFLLLNS +PLGV
Sbjct: 129 VAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGV 188

Query: 181 CVAASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIV 240
           CV  SPLAR V+ VEE   LE GF  +FVITIATG FA +TS+GS+SR LS LS LVGI+
Sbjct: 189 CVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGLVGIM 248

Query: 241 VLLLLPLMVAAAEKVKESVVERKERRVYDFTGD--GDLERV-RPKNEVK---SGDG-AME 300
           V L+LPL+V  AEKVKE V E KE RVY FT +   D+ER+ R +NE K   + +G AME
Sbjct: 249 VFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEGEAME 308

Query: 301 EIGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFG 360
           EIGA EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR S++VSSLVSLSS+FGFFG
Sbjct: 309 EIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFFG 368

Query: 361 RLLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTG 420
           RLLPS+LD+F SR  +    +  G M  LM  +CGGFFLL  PS+ SLC+STAIIA CTG
Sbjct: 369 RLLPSILDYFLSRRNKF-MKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIAICTG 428

Query: 421 AITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPS--AAAGKCMG 480
           AITSISVSTTT LFGA NFS+NHN+VVANIP GSF++GYMAAFLYH       AAGKC+G
Sbjct: 429 AITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAGKCIG 488

Query: 481 VECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 499
           VECYRTTFLIWGC  SFGT LALLLF RTRSFY
Sbjct: 489 VECYRTTFLIWGCFSSFGTFLALLLFARTRSFY 519

BLAST of MC03g0026 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 695 bits (1793), Expect = 3.16e-247
Identity = 374/506 (73.91%), Postives = 421/506 (83.20%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLK  LSISQ QLNNLAFASDAGKLF  +SG+AA
Sbjct: 7   QWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLW VL IGS+LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCY+
Sbjct: 67  NYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHS-PSYCLIFFLTVLAGNSICWINTVCYI 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPS---KRAETFLLLNSAVPLGVCV 180
           VAIRNF  N Q+AVGISTSYQGLSAK+YTDIV  +F     K AETFLL NS  PLGVCV
Sbjct: 127 VAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVCV 186

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
             SP+AR+VE VEE  NLE GF  +FVITIATGVFAVVTS+GSISR LSPLS LVGI+V 
Sbjct: 187 LVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGILVF 246

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDGDLERVRPKNEVK----SGDGAMEEIGAM 300
           LLLP++V   E VK+SV E KERRVY  T + D  R+RP+NE K     GD AMEEIGAM
Sbjct: 247 LLLPVVVPVVESVKKSVREGKERRVYHSTMEEDT-RIRPENEAKLNRDGGDEAMEEIGAM 306

Query: 301 EMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRLLPS 360
           EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAESR S +VSSLVSLSS+FGFFGRL+PS
Sbjct: 307 EMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFFGRLMPS 366

Query: 361 LLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTGAITSI 420
           LLDHF SR  R    +  G + ALM  MCGGFFLL  P++  LCVSTA+IA CTGAITSI
Sbjct: 367 LLDHFLSR--RKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGAITSI 426

Query: 421 SVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGKCMGVECYRTT 480
           SVSTT++LFGA NFS+NHN+VVANIP+GSFL+GYMAA+LY    +  AGKC+GVECYRTT
Sbjct: 427 SVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATGGAGKCIGVECYRTT 486

Query: 481 FLIWGCLCSFGTLLALLLFLRTRSFY 499
           F+IWGCLCSFGTLLALLLF RTRSFY
Sbjct: 487 FMIWGCLCSFGTLLALLLFARTRSFY 508

BLAST of MC03g0026 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 676 bits (1745), Expect = 9.31e-240
Identity = 378/513 (73.68%), Postives = 422/513 (82.26%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLKQQLS+SQLQLNNLAFASDAGKLF   SG+AA
Sbjct: 9   QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 68

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLWLVL IGS+LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCYM
Sbjct: 69  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHS-PSYWLIFFLTVLAGNSICWINTVCYM 128

Query: 121 VAIRNF-SFNPQLAVGISTSYQGLSAKVYTDIVESMFP----SKRAETFLLLNSAVPLGV 180
           VAI NF S N Q+AVGISTSYQGLSAKVYTDIV S+F     SK AETFLLLNS +PLGV
Sbjct: 129 VAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSVLPLGV 188

Query: 181 CVAASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIV 240
           CV  SPLAR V+ VEE   LE GF  +FVITIATG FA +TS+GS+SR LS LS LVGI+
Sbjct: 189 CVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSGLVGIM 248

Query: 241 VLLLLPLMVAAAEKVKESVVERKERRVYDFTGD--GDLERV-RPKNEVK---SGDG-AME 300
           V L+LPL+V  AEKVKE V E KE RVY FT +   D+ER+ R +NE K   + +G AME
Sbjct: 249 VFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERTDEGEAME 308

Query: 301 EIGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFG 360
           EIGA EMVKRINFWLYFGVY FGATLGLAFLNNLGQIAESR S++VSSLVSLSS+FGFFG
Sbjct: 309 EIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFFG 368

Query: 361 RLLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTG 420
           RLLPS+LD+F SR  +    +  G M  LM  +CGGFFLL  PS+ SLC+STAIIA CTG
Sbjct: 369 RLLPSILDYFLSRRNKF-MKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAIIAICTG 428

Query: 421 AITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPS--AAAGKCMG 480
           AITSISVSTTT LFGA NFS+NHN+VVANIP GSF++GYMAAFLYH       AAGKC+G
Sbjct: 429 AITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAAGKCIG 488

Query: 481 VECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 499
           VECYRTTFLIWGC  SFGT LALLLF RTRSFY
Sbjct: 489 VECYRTTFLIWGCFSSFGTFLALLLFARTRSFY 519

BLAST of MC03g0026 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 676 bits (1745), Expect = 4.50e-239
Identity = 369/507 (72.78%), Postives = 414/507 (81.66%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSS LK  LSISQ QLNNLAFASDAGKLF  +SG+AA
Sbjct: 61  QWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFACVSGLAA 120

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLW +L IGS LGL+GYG+Q+LF+T+Q HS PSY LIF LS+LAGNSICWINTVCY+
Sbjct: 121 NYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHS-PSYCLIFFLSVLAGNSICWINTVCYI 180

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPS---KRAETFLLLNSAVPLGVCV 180
           VAIRNF  N Q+AVGISTSYQGLSAK+YTDIV  +F     K AETFLL NS  PLGVCV
Sbjct: 181 VAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLGVCV 240

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
              P+AR+VE VEE  NLE GF  +FVITIATGVFAVVTS+GSISR LSPLS LVGI+V 
Sbjct: 241 LVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGILVF 300

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDGDLERVRPKNEVK----SGDGAMEEIGAM 300
           LLLP++V   E VK+SV E KERRVY  T + D  R+RP+NE K     GD AMEEIGAM
Sbjct: 301 LLLPVVVPVVESVKKSVREGKERRVYHSTMEEDT-RIRPENEAKLNRDGGDEAMEEIGAM 360

Query: 301 EMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRLLPS 360
           EM+KR+NFWLY GVYFFGATLGLAFLNNLGQIAES  S +VSSLVSLSS+FGFFGRL+PS
Sbjct: 361 EMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSFGFFGRLMPS 420

Query: 361 LLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFF-LLWWPSNASLCVSTAIIAGCTGAITS 420
           LLDHF SR  R    +  G + ALM  MCGGFF LL  P++  LCVSTA+IA CTGAITS
Sbjct: 421 LLDHFLSR--RKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTGAITS 480

Query: 421 ISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGKCMGVECYRT 480
           ISVSTT++LFGA NFS+NHN+VVANIP GSFL+GYMAA+LY    +   GKC+GVECYRT
Sbjct: 481 ISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGDTGKCIGVECYRT 540

Query: 481 TFLIWGCLCSFGTLLALLLFLRTRSFY 499
           TF+IWGCLCS GTLLALLLF RTRSFY
Sbjct: 541 TFMIWGCLCSLGTLLALLLFARTRSFY 563

BLAST of MC03g0026 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 4.52e-233
Identity = 369/512 (72.07%), Postives = 415/512 (81.05%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSL+ IIWLQSINGTNFNFPSYSSQLKQQLS+SQLQLNNLAFASDAGKLF   SG+AA
Sbjct: 10  QWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 69

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N+LPLWLVL IGS+LGL+GYG+Q+LF+T+Q HS PSY LIF L++LAGNSICWINTVCYM
Sbjct: 70  NYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHS-PSYWLIFFLTVLAGNSICWINTVCYM 129

Query: 121 VAIRNF-SFNPQLAVGISTSYQGLSAKVYTDIVESMFP----SKRAETFLLLNSAVPLGV 180
           VAI NF S + Q+AVGISTSYQGLSAKVYTDIV S+F     SK AETFLLLNS +PLGV
Sbjct: 130 VAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSVLPLGV 189

Query: 181 CVAASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIV 240
           CV  SPLAR V+ VEE   LE GF  +FVITIATG+FA +TS+GS+SR LS L  LVGI+
Sbjct: 190 CVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGGLVGIM 249

Query: 241 VLLLLPLMVAAAEKVKESVVERKERRVYDFTGD--GDLERVRPKNEVK---SGDG-AMEE 300
           V L+LPL+V   EKVKE V E KE +VY FT +   D ER+R +NE K   + DG AMEE
Sbjct: 250 VFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDGEAMEE 309

Query: 301 IGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGR 360
           IGA EMVKRINFWLY GVY FGATLGLAFLNNLGQIAESR S++VSSLVSLSS+FGFFGR
Sbjct: 310 IGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFFGR 369

Query: 361 LLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTGA 420
           LLPS+LD+F SR  +    +  G M  LM  +CGGFFLL  PS+ SLC+STAIIA CTGA
Sbjct: 370 LLPSILDYFLSRRNKF-MKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIAICTGA 429

Query: 421 ITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAA--GKCMGV 480
           ITSISVSTTT LFGA NFS+NHN+VVANIP GSF++GYMAAFLY          GKC+GV
Sbjct: 430 ITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGVDPGKCIGV 489

Query: 481 ECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 499
           ECYRTTFLIWG   SFGT LALLLF RT+SFY
Sbjct: 490 ECYRTTFLIWGSFSSFGTFLALLLFARTKSFY 519

BLAST of MC03g0026 vs. ExPASy TrEMBL
Match: A0A6J1KBR5 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111492867 PE=4 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 2.20e-227
Identity = 352/503 (69.98%), Postives = 400/503 (79.52%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           QWLSLV IIWLQSINGT+FNFPSYSSQ K QLS+SQLQLNNLAFASDAGKLFGF+SG AA
Sbjct: 7   QWLSLVGIIWLQSINGTSFNFPSYSSQFKHQLSMSQLQLNNLAFASDAGKLFGFVSGFAA 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
           N LPLWLVL IGS+LGL+GYG+Q+LF+ +QLH  PSY +IFLL++LAGNSICWINTVCY+
Sbjct: 67  NSLPLWLVLVIGSSLGLVGYGIQYLFIINQLHD-PSYWVIFLLTVLAGNSICWINTVCYI 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFP---SKRAETFLLLNSAVPLGVCV 180
           VAIRNF  +  +AVGISTSYQGLSAK+YTDIVESMF    SKR ETFLLLNS +PLGVCV
Sbjct: 127 VAIRNFKAHRHVAVGISTSYQGLSAKIYTDIVESMFSKRSSKRTETFLLLNSVLPLGVCV 186

Query: 181 AASPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIVVL 240
             SPL RV   VEE   +E G   +F+ITIATG FAV TS+GSIS  LSPL+ LVGI+V 
Sbjct: 187 FVSPLVRV-HVVEEQGGMEIGVFLIFMITIATGFFAVFTSLGSISTKLSPLTGLVGILVF 246

Query: 241 LLLPLMVAAAEKVKESVVERKERRVYDFTGDGDLERVRPKNEVKSGDGAMEEIGAMEMVK 300
           LLLPL VA AE VK    + ++ R       G +E             AMEEIGA EM+K
Sbjct: 247 LLLPLAVAVAENVKRKTEQPEKHR-------GGVE------------AAMEEIGAKEMLK 306

Query: 301 RINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFFGRLLPSLLDH 360
           R+NFWL+FGVYFFGATLGL FLNNLGQIAESRRS+ VSSLVSLSS+FGFFGRL PSLLDH
Sbjct: 307 RMNFWLHFGVYFFGATLGLVFLNNLGQIAESRRSSGVSSLVSLSSSFGFFGRLTPSLLDH 366

Query: 361 FF-SRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTGAITSISVS 420
           FF  ++ ++      G M AL+  MCGGFFLL WP++ SLC STAIIAGCTGAITSI+VS
Sbjct: 367 FFPGKLMKS----KPGWMVALLGAMCGGFFLLLWPTDTSLCTSTAIIAGCTGAITSIAVS 426

Query: 421 TTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGKCMGVECYRTTFLI 480
            TT+LFGA NFS+NHNLVVANIP+GSFL+G +A FLY +   A AGKC+GVECYR  FLI
Sbjct: 427 VTTELFGADNFSINHNLVVANIPLGSFLFGSLAGFLYRNQVPAGAGKCVGVECYRAVFLI 484

Query: 481 WGCLCSFGTLLALLLFLRTRSFY 499
           WGCLC  GTLLAL+LF RTRSFY
Sbjct: 487 WGCLCLVGTLLALVLFARTRSFY 484

BLAST of MC03g0026 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.6e-115
Identity = 246/514 (47.86%), Postives = 333/514 (64.79%), Query Frame = 0

Query: 2   WLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAAN 61
           WLSLVAI+WLQS NGTN +FP+YSSQLK+ L ISQ +LN L+FASDAGK+ GF+SG+AA 
Sbjct: 8   WLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGIAAV 67

Query: 62  HLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYMV 121
           +LPL LVL  G +LG  GYGLQ+L +  ++                   ICWINT CY+V
Sbjct: 68  YLPLPLVLLAGGSLGFAGYGLQYLSIIKKI-------------------ICWINTACYIV 127

Query: 122 AIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPSKRAET---FLLLNSAVPLGVCVA 181
           AI +F  N Q+AVGI+ SYQGLS K+YTD+V S F + + E    +LLLNS VPL  C+ 
Sbjct: 128 AINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVACLV 187

Query: 182 ASPLARVVEG---VEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGIV 241
            +P+     G   +  +++++ GF+ +FV+TIATG++AV TS+ S+   L    VLVGI 
Sbjct: 188 TAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVL----VLVGIA 247

Query: 242 VLLLLPLMVAAAEKVKESVVERK-ERRVYDFTGDGD----LERVRPKNE---VKSGDGAM 301
           + LL PL +      KE +  RK +++V+D     D    +E    K E    K+  G  
Sbjct: 248 LFLLAPLAIPIGVGFKELMSSRKTQQKVHDLEAPVDKFYFVEEDHTKEEEEFEKAIIGVK 307

Query: 302 EEIGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSSLVSLSSAFGFF 361
           EE+   ++ K+++FW+YFG+Y FG T+GL F NNLGQIAESR S   SSLV+LSS+FGFF
Sbjct: 308 EEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATSSLVALSSSFGFF 367

Query: 362 GRLLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCT 421
           GRLLPSLLD+FFSR       +    MA  +V M   F LL   S+ +L + TA+I   +
Sbjct: 368 GRLLPSLLDYFFSR--NKYMPSSPVSMAGSLVAMVASFLLLLIDSDIALYIGTAMIGIFS 427

Query: 422 GAITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAA--GKCM 481
           GA+TS+SV+ T +LFG K+F VNHN+VV +IP+GSF +G +AA +Y    +     GKC 
Sbjct: 428 GALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRDGAALYGDDGKCF 487

Query: 482 GVECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 500
           G+ C++TT + WG LCS   LLA +L++R R FY
Sbjct: 488 GMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFY 496

BLAST of MC03g0026 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 285.8 bits (730), Expect = 6.7e-77
Identity = 209/560 (37.32%), Postives = 302/560 (53.93%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           +W+ LVA IW+Q+  GTNF+F +YSS LK  L ISQ+QLN LA ASD GK+FG+ SG+A 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
            + PLW VL   + +G +GYG+Q+L +T+ +  S  Y L+FL  LLAG SICW NTVC++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVI--SLPYILVFLCCLLAGLSICWFNTVCFV 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPSKRAETFLLLNSAVPLGVCVAA- 180
           + IRNF  N  LA+ ++ S+ G+SA +YT    ++ P    E +LLLN+ VPL V  AA 
Sbjct: 127 LCIRNFPANRSLALSLTVSFNGVSAALYTLAYNAINPVS-TELYLLLNALVPLFVSFAAL 186

Query: 181 SPLAR-------VVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLV 240
            P+ R         +GV  +  +   F+ + ++ +  GV+ ++      S   S   +  
Sbjct: 187 IPILRQPPLEPLPPDGVRRDSLM---FLLLNILAVLNGVYLLLFR-SKTSDVTSARLLFG 246

Query: 241 GIVVLLLLPLM------------------------------VAAAEKVKESVVERKERRV 300
           G ++LL+LPL                               V   E  K  V        
Sbjct: 247 GSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREASLEG 306

Query: 301 YDFTGDGDLERVR---PKNEVKSGDGAM--------------EEIGAMEMVKRINFWLYF 360
           Y    D  +  V     K+ ++  DG                EE     ++ R +FWLY+
Sbjct: 307 YQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFWLYY 366

Query: 361 GVYFFGATLGLAFLNNLGQIAES-RRSANVSSLVSLSSAFGFFGRLLPSLLDHFFSRMWR 420
             YF G T+GL + NNLGQIA+S  +S+  ++LV+L S+F FFGRLL +  D+  ++++ 
Sbjct: 367 IAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYIRAKVYF 426

Query: 421 AGAGAGAGRMAALMVGMCGGFFLLWWPSNASLCVSTAIIAGCTGAITSISVSTTTQLFGA 480
           A  G  A    AL+      F L    S A+L   TA+I   +G I + +VS T++LFG 
Sbjct: 427 ARTGWLA---VALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELFGP 486

Query: 481 KNFSVNHNLVVANIPIGSFLYGYMAAFLYHSHPSAAAGK-----CMGVECYRTTFLIWGC 500
            +  VNHN+++ NIPIGS +YG++AA +Y SH S A  K     CMG +CY  TF+ WGC
Sbjct: 487 NSVGVNHNILITNIPIGSLVYGFLAALVYESH-SVAGSKTESVICMGRDCYLQTFMWWGC 546

BLAST of MC03g0026 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 6.2e-75
Identity = 197/532 (37.03%), Postives = 298/532 (56.02%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           +W  LVA IW+Q+  GTNF+F +YSS LK  L ISQ++LN LA ASD GK FG+ SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
            + PL +VL   + +G +GYG+Q+L +T+ +  +  Y L+FL  LLAG SICW NT C++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNII--TLPYSLVFLCCLLAGLSICWFNTACFI 162

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPSKRAETFLLLNSAVPLGVCVAA- 180
           + IR+F  N  LA+ ++ S+ G+SA +Y+    ++ PS  +  +LLLNS VPL V  AA 
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSS-SNLYLLLNSLVPLVVSFAAL 222

Query: 181 -----SPLARVVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLVGI 240
                 P        +  R+    F  + V+ + T    +++S  + S  L+ +  +V +
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLL 282

Query: 241 VVLLLLPLMVAAAE----------------KVKESVVERKERR--VYDFTGDGDLERVRP 300
           V  L  PL+V A +                 V  ++ E K ++  V   TG   +   + 
Sbjct: 283 VFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKE 342

Query: 301 KNEVKSGDGAMEEIGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAESRRSANVSS 360
            N V+ GD    E     ++ R+ FWLY+  YF G T+GL + NNLGQIA+S    N ++
Sbjct: 343 GNTVRLGD----EHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQS-LGQNSTT 402

Query: 361 LVSLSSAFGFFGRLLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWPSN--A 420
           LV++ S+F FFGRLL +  D F  + +R       G  A  ++     FFLL   S+   
Sbjct: 403 LVTIYSSFSFFGRLLSAAPD-FMHKRFRL---TRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 421 SLCVSTAIIAGCTGAITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYH 480
           +L  +TA+I   +G I + +VS T+ LFG  +  VNHN+++ NIPIGS LYGY+AA +Y 
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE 522

Query: 481 SHPS-------AAAGKCMGVECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 500
           ++ S       + +  C+G +CY  TF+ WGCL   G + +L L++RT+  Y
Sbjct: 523 ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MC03g0026 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-74
Identity = 204/563 (36.23%), Postives = 305/563 (54.17%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           +W+ LVA IW+Q+  GTNF+F +YSS LK  L ISQ+QLN LA ASD GK+FG+ SG+A 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
            + PLW VL   + +G +GYG+Q+L +TH +  S  Y ++FL  LLAG SICW NTVC++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITHFI--SLPYIMVFLCCLLAGLSICWFNTVCFV 126

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMFPSKRAETFLLLNSAVPLGVC-VAA 180
           + I NF  N  LA+ ++ S+ G+SA +YT    ++ P+   E +LLLN+ +PL V   A 
Sbjct: 127 LCISNFPANRSLALSLTVSFNGVSAALYTLAYNAINPTS-PELYLLLNALIPLIVSFTAI 186

Query: 181 SPLAR-------VVEGVEENRNLEGGFVAMFVITIATGVFAVVTSIGSISRALSPLSVLV 240
            P+ R         +GV  +  +   F+ + ++    GV+ ++   GS S  L+   +L 
Sbjct: 187 IPILRQPPFEPLPPDGVRRDSLM---FLLLNILAALNGVYLLL--FGSNSSDLTSARLLF 246

Query: 241 GIVVLLL-----LPLMVAA--------------------------AEKVKESVVERKERR 300
           G  +LLL     +P +V A                           E  K  +     R 
Sbjct: 247 GGAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANRE 306

Query: 301 VYDFTGDGDLERVRPKNEVKSGDG------------AMEEIGAME----MVKRINFWLYF 360
            Y    D  ++       V+  D              +E +G       ++ R +FWLY+
Sbjct: 307 GYQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYY 366

Query: 361 GVYFFGATLGLAFLNNLGQIAES-RRSANVSSLVSLSSAFGFFGRLLPSLLDHFFSRMWR 420
             YF G T+GL + NNLGQIA+S  +S+N ++LV+L SAF FFGRLL +  D+  ++++ 
Sbjct: 367 ITYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYIRAKVY- 426

Query: 421 AGAGAGAGRMAALMVGMCGGFFLLWWPSNAS-LCVSTAIIAGCTGAITSISVSTTTQLFG 480
               A  G +A  ++      FLL     AS L   TA++   +G I + +VS T++LFG
Sbjct: 427 ---FARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFG 486

Query: 481 AKNFSVNHNLVVANIPIGSFLYGYMAAFLYHSH-------PSAAAGKCMGVECYRTTFLI 500
             +  VNHN+++ NIPIGS +YG++AA +Y SH        ++ +  CMG +CY  TF+ 
Sbjct: 487 PNSVGVNHNILITNIPIGSLIYGFLAALVYDSHGFTGTKSMTSESVVCMGRDCYYLTFVW 546

BLAST of MC03g0026 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 8.2e-67
Identity = 190/531 (35.78%), Postives = 283/531 (53.30%), Query Frame = 0

Query: 1   QWLSLVAIIWLQSINGTNFNFPSYSSQLKQQLSISQLQLNNLAFASDAGKLFGFLSGVAA 60
           +W  LVA IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK  G+ SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 61  NHLPLWLVLTIGSTLGLLGYGLQFLFLTHQLHSSPSYPLIFLLSLLAGNSICWINTVCYM 120
            + P+  VL   + +GL+GYG+Q+L +   +     Y L+ +   LAG SICW NTV ++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVI--DLPYSLVLVCCSLAGLSICWFNTVSFI 142

Query: 121 VAIRNFSFNPQLAVGISTSYQGLSAKVYTDIVESMF--PSKRAETFLLLNSAVPLGVCVA 180
           + IR+F  N  LA+ +  S+ G+SA +YT   E++    S  ++ +LLLNS +PL V V 
Sbjct: 143 LCIRHFKANHSLALSLVVSFNGISAALYTLGHEAISGKSSASSDIYLLLNSLIPLIVSVL 202

Query: 181 AS-PLARVVEGVEENRNLEGG----FVAMFVITIATGVFAVVTSIGSISRALSPLSVLVG 240
           A  P+       E +          FV   V+ + T  + ++ S G+   A SP    +G
Sbjct: 203 ALWPVLTNPNSSETDTTRTHDETRIFVVFNVLALVTCFYLLLPSSGTY-LASSPRWHFLG 262

Query: 241 IVVLLLLPLMVAAAEKVKESV-------------VERKERRVYDFTGDGDLERVRPKNEV 300
            + LLL PL V   + +  ++             V  +E ++               ++V
Sbjct: 263 AIFLLLFPLCVPFLDYIHRALESCFHHHSSGYAAVNIEEPKILKIKSQKS-NAEEESDQV 322

Query: 301 KSGDGAMEEIGAMEMVKRINFWLYFGVYFFGATLGLAFLNNLGQIAES--RRSANVSSLV 360
           + GD    E     +V+++ FWLY+  YF G T+GL + NNLGQIA+S  + S+N  SLV
Sbjct: 323 RLGD----EHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLV 382

Query: 361 SLSSAFGFFGRLLPSLLDHFFSRMWRAGAGAGAGRMAALMVGMCGGFFLLWWP---SNAS 420
           +L SAF F GRLL S  D  F+R              +L+      F L + P     A 
Sbjct: 383 TLFSAFSFLGRLLSSAPD--FTRKKLKYLTRTGWFTISLLPTPLAFFILAYSPKTNQTAL 442

Query: 421 LCVSTAIIAGCTGAITSISVSTTTQLFGAKNFSVNHNLVVANIPIGSFLYGYMAAFLYHS 480
           L V+TA+I   +G + + +VS T++LFG  +  VN N+++ NIPIGS  YGYMA  +Y +
Sbjct: 443 LEVATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDT 502

Query: 481 HPS-------AAAGKCMGVECYRTTFLIWGCLCSFGTLLALLLFLRTRSFY 500
           + S       A +  C+G +CY  TFL WGCL   G + +L LF+RTR  Y
Sbjct: 503 NASLGRKSVVADSVVCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVY 543