MC03g0025 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC03g0025
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pseudouridine synthase
Location	MC03: 354538 .. 361372 (+)
RNA-Seq Expression	MC03g0025
Synteny	MC03g0025

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTCTTAAGCGGGCCTCATGGACATCGCGATCCAAGATCAGTAGCTAAAACAGCCTGAGCCAGCTATGGTACTCTCCAGTGTCCACACAGCGGAGATTGGGTGCCACTGCCATGGCAGTCTCTTCTCTGCGGTTCCCGCTTTCCCCATGGACGAAAACTTCATCTACATGCTTCCGCTTACCCTACATTGCTTTCGTTTCGAGGAGCCCACATCTTAGACCTAGACTATTGTGCTGTTGCTCATCTCCTTCTGCGTCCACTGACGCTTCTTCCACTCGCTGCGCTCCGGACCAGCTCCGGACATGGGAAGCTTTTCGCAAGAAAAAGGTCGTTATGCGGATCGGCTATGCCGGGACTGATTATAGAGGTCCCATTTCGTATTACTCTCTATTTCTCTTGACGACCCTGTTGCAAAATATTGAGTGTTTTACCAGTTCATTAATATTTGAATTTTTCAAATTCAGGACTGCAAATGCAACGCGAGCAACAGTCACATCCAAGTAAGTTTCTGTTTTACACCCATTCCTTTGTTAATCGCAATTTCCTGTCATTGTATTGGCAATAACTGCCGCTGGATATTTTGTAGCTGTGTTTGGTAATTGTGAACTTTCACAGTAAACGTGACTGTGATATTAGAAATTACTGATTTAGCTAACTGCAGGCTACAACAACTATTTATGACAATCTAGTCTGAAATTTATACTAGTTACAGAGTAACTAACTTAAACAGTCGAGCTTCTCTAAGCAATGAGAAATTTTATATATATATATATAAAAGAACAAATTTAAATACATTTGAGCTAATTAACCTCGTATATACTTAAAATCCCCCCGGGGGTGTTGGATCTCAAACAGCAGTTGATTACTGAAAGTGGCCTTTTGAGTATATCAGCCGTCTGATTACGATATTTTGAATTGCTAATGACCCAAGAAAACTTTCTCAATCTCAATGTCTCTTATTCAAGAATATTGAACTGAGTCGAGGATGAGTTTGGCATACCTTTAAGTTTAAACATTTTTTCGTAAAGTACTCCTAATAAATATAATAATTATGCATTTGGTTACAATATTTTAAAAAGTGTTTTTAAAAACAAATTGGAGTGCAATCATTATAGGTGGCTCATTGGTCCATAGGGGCTAGGGTAATCTCATGATGGCCACCTACCTTAGAAATTACTAATGTTGTAGGGTCAGACGGTTGCCCCGTGAGATTAGTCGAGGTGCGCCTGCGCATAAGCTGGTCCGGACACTCACGGATATAAAAAAAAAAAGAACAAATTGGAGTGCTCAACACAAGTTATTTTGTAACTATTCAACCTATACCATCGGTTTTGATTGGAGGGCTCTTATACAACACTCCCTGGGAGAAGGGACTACTTGTCCCTTATTCATTTAGGCTGTCTTTGCCTTGACAATTATTTGAATGAAAGCATTACTCTATTTCTTATTAAAAAGAAAAAGAATTTATTTGGTTGCACTACATTAGTGATTCTACTAATCACAAATATATTTTTCGAATATGTTTTTTTCTTAATATTGATTTTCAGTCGATCACTTTAAAGCAGCCTTTTGAATAATCATCTTTAAAGTGCGTCCTCCGAAAGAGATTTGTAAATCAATATGGCTTTTTAATATGCTTTTTATTATCATAGATTTTTAAGAAAGCACTTCTAGTCATCTAAAACCACTTCGAAAGTTAACCATCATGGGTTGGCCTAGTGGTCAATAAGGGTCAATGTAAATAGTAAAGGGCTTAGAGGGAGTCATGGTGACCACCTGCCTAAGATTTAATATCCTACGAGTTACCATGACAACCAAATGTAGTAGATCAAACACACACTTAAGTCCGTACAAGATTAGTTGTAATGTTGGCTATGGTCTGTAGGTTGCAGCACCTCCAAAGATAATGCATACACATGTATCAGTATCAAAATCAACTATGGTTATGAGACTTAGAGATTCAATTTTGTCTTTTTTTAGAAAGAGGGTTAGAGGTTCAATATGAATCAGCTAACCCATCAATCTTTAATGAAGTTGGTCTGCTTAAATAAAGACCAAATTATCAGCTCCTTTAATGGACCGCAACGTATCTTTTTCTGCATTCTAATGAGTCTTTGGGGGGGGGGGGGGGGGTGNTTGGGCAAGGGATTGGGTTTTATAAACCCACCAATGCATTTGGATTTACATTAGAATCTTGTGAGGTTGGGTTTTTGTAGACTTTTTGCTAGCATTCAAGTTAATGCTAAACCATGGGATTCATCCATTTCAAAGGTTGTTGTCCACATAAAAGTTGACTATTGGAGCTTACCATTTTTCATAACTTGTAGCAAGAATTTCATTTTGCTATAATAGTCTTACATTGAGAAATTGTGTTACTTTTGTCCTTGAAAAGTTGATCCTTGTACTTTATGACCTTCACTTTACACCTCGTAGTGAAAACCTGTTTCAAAGCACTTCACAAGATGCAGGAAGATTTACAACTTGAGTCCTAATGCTTTCAATTGTTGAGGCAAGTAACCGTGACTATCATTTTGTCTTAGATTACAAGCACAATAATCTGGATTCCGACTTGATTTATCAGCATTTCTAAAGTGTATGAAAGTGGGTGGATATTTGAAAGATCCATCATCATAGCATTCACGGTTGAAGCCTTTTATTGCAACTAGGCTTGAAACTTATTCAGAAGGAACTTTTTATCTCAGCCTTCCTATGATGTACTTACTAGTCGATTGGACTTGCCTGTATTAAAGACAATGTGATCTAAGAGGCAACTTTTTAAAGGGAAGTGTTTGGATGTTACATCTTCTGGAGAACTAGGGATAGACACTAAGGTGGCAGAATAGTTACATGTTAAAAGAGTCTATAAAAAGAACATGTACCAGCAAAACTGATACCATGTAAACTTTGTAAGCAATAATGAGCTGATATTCTCAGAGTCACCATGTATAAGGTAGGACAACTATTCAACGATCTAGTTACGTACTAACCAACAGAAACAGTAGAGCTTAATATGTAGTCAATAGATGAATTGCTGATTAAATACACTTAAACTAATTACTCACTTAATATTTAACTAACCTTGACATTGATATAGCATTTTATGTACATTAATCCCTAAGGATCTGTTGAGACTTCCATTCATTTTATGTATTCATTTATTTATTTGCTCTTATTATAAATCTCACTTCTCTTACCTGAGAAAGTATAACAAACTGAAGAAGGCTATAGATACTTCTATTCTATATCTCTTCCTGTAATTTATCAAATATATTCTGGACATCGCCTGACGCCTCATCTTCACACCGAAGTATAGAGAACCTAACTGCTTTGGATGAACACTACTTATTTTCTCACACTTCTTTGGGTATCCCAGATAAGCATTTCTTTGCCTTCCCACGTTTCTTTTACCATAAACAATGTTAAATGCCCCTGTTCATTATGATGTGGATGATACTGAGTTAATAATATGCCTGGAATGTGAGGTAATATTGTTTCTTTTTTTCTTTTGCTGGCAAATTACAACACAGAAATTAACCTCATACATACACTCCAAACTACCCAGCTAGTGCATGGATGGGATGTCCTAAAGCATTACAGTTTTGATGTGTAGCAATCGAACAAGAATTGGAAGCTGCAATATTTAAGGCTGGTGGAATTCGTGATAGTAACTATGGAAATCTGTACAAAATCTCATGGGCTAGGAGCAGTCGAACTGATAAAGGAGTAATTATCTTCTTCACTGTATCTCATTTATGGCTCAAGTATGGTTATTGATTGTTTTGATTTGTTCTTTCTTCTCTATTTTTCATTTTTAAGGTTCATTCCTTGGCCACAACAATTTCCTTAAAAATGGAAATCCCTGAAAATGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATAAACTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATCGTACCTTCACAGAGGTGAGAGTTTGAGTTTTTCCTTTGGTTCGTTTAAGTGAGTAAATGAAGTTTCAGCATTAGCATGATGAACTTTTATGCCTTTACTACAATGTGGTATTTACATCAATTTTGTCACTTGTCAAGGAGCTTTGATCCTCGAAGGGAATGTGATGTCCGGAAGTACTCTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAGAGTAAATTTAACACTACTGAAATTGATCACCACATTTCAGAGTTTAAAGATATATTAAACTCCTTTCAGGTGGGTTTCTATCTCCTTCATGTAATTACATAAAGGTCAGATTGTCCCTGAAGATAATATGTGCAGGGCACAGCAAGGCTCATACTTTGTTATTATTTGATATAGCTTAGTACTTTTATTGTGAAGACAATTGTATTGTATTATTGAAGAACATAGGTGGATTTTTTTTTTTTTTTTTTGTGGGGTACAGGGAGAGCACCCTTTCCATAACTATACAATACGTTCCAAGTATAGAAAGACAATTCGAGCCAGGCAATCTCCTGGATATGGCCATAATTCAGGAGAAGAAGTATCACCTAGTGGTATGTCGAACTCTGGGTTTGAGGAGAGTGATAGTGAAGATAATTTTTTTGTCGAAGGAGTCAAGGGGCCAAATCAACATGCTCCAAGATTAAGTGTTTGTTGTGAAGATCCAGTTGAAGTATGCAGTAAGCTTGGAAATGGATCAGAGCTTCAAAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGCATAGGTGCCTCCCACTTCAGAAAGATTCTTTGCTGTTCTTGTGGGAAACTTGAAACATCTCTTGGATACGATTTCGTTGAGATCTCAATCTGGGGAGAATCTTTCATGTTGCATCAAGTTAGTCTTTCCCAAACTTTGATTACATAACTTATTCCCATCTATTTCTCGTTCTAATTTGTAAGTTTATCTATGTATACGTCATATATATCCCCATCTACTGTAGGCTATTTTGCTTGGATTTGGTTGGGTTCCTGTTCTTGCACGTCCAGAACTTGGTTTTATAACTGTTTCTTTTCCCCTCGCAATAATTGTTTTGCAGCGGCATTTCAGTTCTTGAACTCTTTTTCCCCTTTGCAAATCAATTATCAGTGGCCTAGTGGTTTTTTTTCCCTGTGCTAGCAGTCTTCAAAATAAATAATGCAATAGCTGAAGATTGATGTATGAATTATGAAATTGAATTCTAAAATACTGCCTTAACTTCTGACCATCAAAGTTTTGATGGTCCCTGTACGTTGGCGGACAGAAAATATGAGTTCAGGCTTTATGAGCTTGACTTTTTCAATATCTACCATACTAACCTTCATTTACTCCCTGAGTTTCATAATTCAGAGAACCAGCTAACACCATAAAACATGTCAAGTTTAACGTACCTAGCGCTATTTGAAATCAATTCTTGTTATCTTGTTTAGGAGGTGGAAACAGAAATTCCGTTCAGACAATTTGATTTGTTTGAAAAGATCTCTCTTCTCTGTTCTGGTTGAGATTAGAGTCATTAGACCATTCCATTGTGGCCAAAGCCTCATGAAGAAACCCAGAATCTTATGGATAAACCGTACAGAAAGGCTTTACTTTGGCAGATTTGAGCAGAGATAAATTATAGAATCTTTTATAGTAATCTAAGGGGTTGGGAAGGGATGTGGGCAATGGCAGGGAGGTGGGCTTCCTTCTGGCAGATTTCTCATTTCCATTTGTGTTTGCACCTTATGAGCTTTAAATATAATTTGTTTCCTACCAAAAATAACAATAATGATGCGTTCTATGAAGAATGAAATTGGCTTCATTTAGTATCTGGTAGTTAATGTTTTGAATTGAGAGGCCTGTACTAATATTGCTTTTATTACATCCTGGACAGATCCGGAAAATGGTGGGAACAGCCGTTGCTGTGAAGCGGAAACTGCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTTTCTAGAATAGTTCTGCCCATTGCACCAGCAGAGGTTTTAGTCCTAAGAGGGAACAGCTTTTCCAACGGAAAACGTCCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTGAACAAATCGGACGACATCATCAAGTCGGTGGACGAATTCTACAGATCAGCGATATTACCTGAGCTAACAAACTTCCTGGACACGGAAAAAGCGCCATGGAACGAATGGGTGGAGAAATTGGACAAATACACAAGCATTCCCAATGAGCAACTGGATGAAGTCAGAAATGCCTGGAAAGCATGGAAGGAAAAGTTTGAGAGTGGACAAACACATAAAAGGTACATTGGTCATGAAGAATTCAAAGCACCCATTTGAATTATGTTGATGTGTTTTTTTGTGTGTGCCTCTTTTGTTGGAAGAAGTCTTTGAAGAGGATGGGAGGCAGTTGAGTTTAGTTGGTGGAGTTATTATTGGTACAAATTATGGGGTTTGGGCATTGAATGCCACTGGCCATGGGGGGCGTTTAAGAGCGCAGCATCAACCACAGACCAACCCCAACCCACTCAGCACCATAACGGAGCTCGCTCGCATTCAATGTCAGCCGCTCATAAGGCGAAATTTGTAGGTGAGCCATGAATCATGATGTTCATTTTACTTGATTTGTGATAACAATTTTGAGTGAGCCTTGACTAATAAACACTGTGAAAAGGATTGCTGTTGCTTCTCTCTTTTAATTATGATTTAAATTGTTTGCTCTTTGTATTATTGAGATACGTGTAAATTGGAATCATCACATGATGAGAGCAAAAAATCTCCATTTAAAATGGAGTCAATATTGATGTGAATTATGAAGTGATTAAATATTGGTATCTCTTATATAAGTTGTTG

mRNA sequence

GTTTTCTTAAGCGGGCCTCATGGACATCGCGATCCAAGATCAGTAGCTAAAACAGCCTGAGCCAGCTATGGTACTCTCCAGTGTCCACACAGCGGAGATTGGGTGCCACTGCCATGGCAGTCTCTTCTCTGCGGTTCCCGCTTTCCCCATGGACGAAAACTTCATCTACATGCTTCCGCTTACCCTACATTGCTTTCGTTTCGAGGAGCCCACATCTTAGACCTAGACTATTGTGCTGTTGCTCATCTCCTTCTGCGTCCACTGACGCTTCTTCCACTCGCTGCGCTCCGGACCAGCTCCGGACATGGGAAGCTTTTCGCAAGAAAAAGGTCGTTATGCGGATCGGCTATGCCGGGACTGATTATAGAGGACTGCAAATGCAACGCGAGCAACAGTCACATCCAACAATCGAACAAGAATTGGAAGCTGCAATATTTAAGGCTGGTGGAATTCGTGATAGTAACTATGGAAATCTGTACAAAATCTCATGGGCTAGGAGCAGTCGAACTGATAAAGGAGTTCATTCCTTGGCCACAACAATTTCCTTAAAAATGGAAATCCCTGAAAATGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATAAACTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATCGTACCTTCACAGAGGAGCTTTGATCCTCGAAGGGAATGTGATGTCCGGAAGTACTCTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAGAGTAAATTTAACACTACTGAAATTGATCACCACATTTCAGAGTTTAAAGATATATTAAACTCCTTTCAGGGAGAGCACCCTTTCCATAACTATACAATACGTTCCAAGTATAGAAAGACAATTCGAGCCAGGCAATCTCCTGGATATGGCCATAATTCAGGAGAAGAAGTATCACCTAGTGGTATGTCGAACTCTGGGTTTGAGGAGAGTGATAGTGAAGATAATTTTTTTGTCGAAGGAGTCAAGGGGCCAAATCAACATGCTCCAAGATTAAGTGTTTGTTGTGAAGATCCAGTTGAAGTATGCAGTAAGCTTGGAAATGGATCAGAGCTTCAAAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGCATAGGTGCCTCCCACTTCAGAAAGATTCTTTGCTGTTCTTGTGGGAAACTTGAAACATCTCTTGGATACGATTTCGTTGAGATCTCAATCTGGGGAGAATCTTTCATGTTGCATCAAATCCGGAAAATGGTGGGAACAGCCGTTGCTGTGAAGCGGAAACTGCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTTTCTAGAATAGTTCTGCCCATTGCACCAGCAGAGGTTTTAGTCCTAAGAGGGAACAGCTTTTCCAACGGAAAACGTCCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTGAACAAATCGGACGACATCATCAAGTCGGTGGACGAATTCTACAGATCAGCGATATTACCTGAGCTAACAAACTTCCTGGACACGGAAAAAGCGCCATGGAACGAATGGGTGGAGAAATTGGACAAATACACAAGCATTCCCAATGAGCAACTGGATGAAGTCAGAAATGCCTGGAAAGCATGGAAGGAAAAGTTTGAGAGTGGACAAACACATAAAAGGTACATTGGTCATGAAGAATTCAAAGCACCCATTTGAATTATGTTGATGTGTTTTTTTGTGTGTGCCTCTTTTGTTGGAAGAAGTCTTTGAAGAGGATGGGAGGCAGTTGAGTTTAGTTGGTGGAGTTATTATTGGTACAAATTATGGGGTTTGGGCATTGAATGCCACTGGCCATGGGGGGCGTTTAAGAGCGCAGCATCAACCACAGACCAACCCCAACCCACTCAGCACCATAACGGAGCTCGCTCGCATTCAATGTCAGCCGCTCATAAGGCGAAATTTGTAGGTGAGCCATGAATCATGATGTTCATTTTACTTGATTTGTGATAACAATTTTGAGTGAGCCTTGACTAATAAACACTGTGAAAAGGATTGCTGTTGCTTCTCTCTTTTAATTATGATTTAAATTGTTTGCTCTTTGTATTATTGAGATACGTGTAAATTGGAATCATCACATGATGAGAGCAAAAAATCTCCATTTAAAATGGAGTCAATATTGATGTGAATTATGAAGTGATTAAATATTGGTATCTCTTATATAAGTTGTTG

Coding sequence (CDS)

ATGGCAGTCTCTTCTCTGCGGTTCCCGCTTTCCCCATGGACGAAAACTTCATCTACATGCTTCCGCTTACCCTACATTGCTTTCGTTTCGAGGAGCCCACATCTTAGACCTAGACTATTGTGCTGTTGCTCATCTCCTTCTGCGTCCACTGACGCTTCTTCCACTCGCTGCGCTCCGGACCAGCTCCGGACATGGGAAGCTTTTCGCAAGAAAAAGGTCGTTATGCGGATCGGCTATGCCGGGACTGATTATAGAGGACTGCAAATGCAACGCGAGCAACAGTCACATCCAACAATCGAACAAGAATTGGAAGCTGCAATATTTAAGGCTGGTGGAATTCGTGATAGTAACTATGGAAATCTGTACAAAATCTCATGGGCTAGGAGCAGTCGAACTGATAAAGGAGTTCATTCCTTGGCCACAACAATTTCCTTAAAAATGGAAATCCCTGAAAATGCATGGATGGGCGACCCTTGTGGTATTTTGCTTGCTAATTACATAAACTTTTATCTTCCTGATAATATCAAGGTTTTTGGCATCGTACCTTCACAGAGGAGCTTTGATCCTCGAAGGGAATGTGATGTCCGGAAGTACTCTTATCTCCTCCCTGCTAAGCTTATTGGAATTGAGAGTAAATTTAACACTACTGAAATTGATCACCACATTTCAGAGTTTAAAGATATATTAAACTCCTTTCAGGGAGAGCACCCTTTCCATAACTATACAATACGTTCCAAGTATAGAAAGACAATTCGAGCCAGGCAATCTCCTGGATATGGCCATAATTCAGGAGAAGAAGTATCACCTAGTGGTATGTCGAACTCTGGGTTTGAGGAGAGTGATAGTGAAGATAATTTTTTTGTCGAAGGAGTCAAGGGGCCAAATCAACATGCTCCAAGATTAAGTGTTTGTTGTGAAGATCCAGTTGAAGTATGCAGTAAGCTTGGAAATGGATCAGAGCTTCAAAAGGGTGTTCGTGCAAGATGGCTTCATGAGCCAGATGAGAAAGATCGCATAGGTGCCTCCCACTTCAGAAAGATTCTTTGCTGTTCTTGTGGGAAACTTGAAACATCTCTTGGATACGATTTCGTTGAGATCTCAATCTGGGGAGAATCTTTCATGTTGCATCAAATCCGGAAAATGGTGGGAACAGCCGTTGCTGTGAAGCGGAAACTGCTCCCAAGAGATATTATAGCATTGTCACTGACCAAGTTTTCTAGAATAGTTCTGCCCATTGCACCAGCAGAGGTTTTAGTCCTAAGAGGGAACAGCTTTTCCAACGGAAAACGTCCAGGAGCTCCAAAGAGGCCCGACTTGCTCACATTGAACAAATCGGACGACATCATCAAGTCGGTGGACGAATTCTACAGATCAGCGATATTACCTGAGCTAACAAACTTCCTGGACACGGAAAAAGCGCCATGGAACGAATGGGTGGAGAAATTGGACAAATACACAAGCATTCCCAATGAGCAACTGGATGAAGTCAGAAATGCCTGGAAAGCATGGAAGGAAAAGTTTGAGAGTGGACAAACACATAAAAGGTACATTGGTCATGAAGAATTCAAAGCACCCATTTGA

Protein sequence

MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPDQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHNYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPRLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVEKLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI

Homology

[Close]

BLAST of MC03g0025 vs. ExPASy Swiss-Prot
Match: O22928 (Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g30320 PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 6.3e-139
Identity = 264/509 (51.87%), Postives = 342/509 (67.19%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60
           MAVS LR P+       S  F  P     +R   + P LL    S SA+   SST  +P 
Sbjct: 1   MAVSFLRIPI------PSRVFWYP-----TRLIRVTPALL--RFSSSAAFQPSSTSLSPP 60

Query: 61  Q-----LRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRD 120
                    WE++RKKKVV+RIGY GTDYRGLQ+QR+  S  TIE ELE AI+KAGGIRD
Sbjct: 61  SSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRD 120

Query: 121 SNYGNLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNI 180
           SNYG+L+KI WARSSRTDKGVHSLAT+ISLKMEIPE AW  DP G +LA  I+ +LP+NI
Sbjct: 121 SNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENI 180

Query: 181 KVFGIVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGE 240
           +VF ++PS R FDPRREC +RKYSYLLP  ++GI++ F + EID+HI++F +IL  F+GE
Sbjct: 181 RVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGE 240

Query: 241 HPFHNYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPN 300
           +PFHNYT RS+YR+    +     G    E  S             SE  F  E      
Sbjct: 241 YPFHNYTQRSRYRRKSEQKIKQRNGRPPREPKSIKA----------SESEFREENHIEIG 300

Query: 301 QHAPRLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKL 360
           +      V  E    V +   + S++    RA+WL+EPDE D+I  +HFRK+  C  GKL
Sbjct: 301 EEEEEKEVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKL 360

Query: 361 ETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPA 420
           E SLG+ FVEISIWGESFMLHQIRKM+GTAVAVKR+LLPRDII LSL KF+RIVLP+AP+
Sbjct: 361 ENSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPS 420

Query: 421 EVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPW 480
           EVL+LRGNSF   +    P+RP +    +S+++ K ++EFYR+ ++P+++ FLD+EK+PW
Sbjct: 421 EVLILRGNSFEVRR---LPERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPW 480

Query: 481 NEWVEKLDKYTSIPNEQLDEVRNAWKAWK 505
            EWV+ LD+   + +E+L++VR  W+ WK
Sbjct: 481 KEWVDHLDRNDGLIDEELEDVRKGWEEWK 481

[Close]

BLAST of MC03g0025 vs. ExPASy Swiss-Prot
Match: Q6CWQ8 (tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 6.7e-24
Identity = 107/356 (30.06%), Postives = 167/356 (46.91%), Query Frame = 0


Query: 70  KKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARS 129
           K+KV + IGY GT Y G+Q       + TIE+EL  A  +AG I  +N  +L K  + R+
Sbjct: 98  KRKVAVMIGYCGTGYHGMQY---NPPNDTIEKELFEAFVRAGAISKANSTDLKKNGFQRA 157

Query: 130 SRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDP 189
           +RTDKGVH+    ISLK+ I       DP    +   IN  LPD I+V+ I    ++FD 
Sbjct: 158 ARTDKGVHAGGNVISLKLIIE------DP---EVKEKINNELPDQIRVWDISRVNKAFDC 217

Query: 190 RRECDVRKYSYLLPA-KLIGIE-SKFNTTEIDHHISEFKDILNSFQGEHPFHNYTIRSKY 249
           R+ C  R Y YLLP   LIG + S +   EI+   +  K+I N  Q +         +++
Sbjct: 218 RKMCSSRWYEYLLPTYSLIGPKPSTYLYNEIE---ASKKEIPNVIQDD------VESAQF 277

Query: 250 RKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDS--EDNFFVEGVKGPNQHAPRLSVCC 309
            +         +     EE++   M    F+E++   + +   + ++  ++ A R+S   
Sbjct: 278 WEAFSTEAESKFTKEELEEIAAFVMPKDSFDENNETYQKSKAFKKLEAEHKRAYRISKER 337

Query: 310 EDPVEVCSKLGNGS-ELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLGYDFV 369
            D      K   G+         +  ++P       A  F K +  S   +      ++V
Sbjct: 338 LDRFRSALKQYEGTFNFHNFTLGKDFNDP------SAKRFMKQITVSEPFVIGEAKTEWV 397

Query: 370 EISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 421
            I I G+SFMLHQIRKM+  A  + R   P + I  +  +  +I +P APA  L+L
Sbjct: 398 SIKIHGQSFMLHQIRKMISMATLITRCFSPLERIRQAYGQ-EKINIPKAPALGLLL 425

[Close]

BLAST of MC03g0025 vs. ExPASy Swiss-Prot
Match: Q59S63 (tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=PUS1 PE=3 SV=2)

HSP 1 Score: 105.5 bits (262), Expect = 1.8e-21
Identity = 111/374 (29.68%), Postives = 172/374 (45.99%), Query Frame = 0


Query: 70  KKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARS 129
           K+KV + +GY GT Y G+Q+Q +  +  TIE+++  A+  AG I   N  +L K  + R+
Sbjct: 148 KRKVAVMLGYCGTGYNGMQVQND-PNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRA 207

Query: 130 SRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDP 189
           +RTDKGVH+    ISLKM I       DP    + N IN  LP  I+++GI  + + FD 
Sbjct: 208 ARTDKGVHAAGNVISLKMIIE------DP---EIINKINDLLPKQIRIWGIQRTTKGFDC 267

Query: 190 RRECDVRKYSYLLPA-KLIGIESKFNTTE-IDHHISEFKDILNSFQ-GEHPFHNYTIRSK 249
           R+ C  R Y YLLP   L+  + K   +E +     E  D+    Q G   + N  ++SK
Sbjct: 268 RKCCSSRIYEYLLPTFSLLPPKPKSVLSELVKEKKLENPDLFEDDQEGIDWWEN--VKSK 327

Query: 250 YRKT-IRARQ----SPGYGHNSGEEVSPSGMSNSGFEESDSEDNFF-----VEGVKGPNQ 309
              + I   Q    +  Y     ++           E   SE +F      ++ ++  ++
Sbjct: 328 ILASGITQEQIDSITSSYDEQQQQQQQQQQQQADEEERDISELSFTKLIKQIKTIENQSR 387

Query: 310 HAPRLSVCCEDPVEVCSKLGNGSELQKGVRA-----RWLHEPDEKDRIGASHFRKILCCS 369
            + R+S          S+L +  E+ K          +      KD        K +   
Sbjct: 388 RSYRIS---------SSRLQHFREVMKQYEGTHNFHNFTVGKPFKDTSANRFMIKTIVSD 447

Query: 370 CGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLP 426
              +E   G +++ I I G+SFMLHQIRKM+  A  V R  LP  II  +  + ++I +P
Sbjct: 448 PFVIE---GTEWISIKIHGQSFMLHQIRKMIAMAALVVRLSLPCGIIN-NFFQSTKINIP 496

[Close]

BLAST of MC03g0025 vs. ExPASy Swiss-Prot
Match: Q6CC39 (tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PUS1 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.4e-21
Identity = 104/368 (28.26%), Postives = 168/368 (45.65%), Query Frame = 0


Query: 70  KKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARS 129
           K+KV   IGY GT Y G+Q+   Q+   TIE ++  A  KAG I  +N  +  K ++ R+
Sbjct: 84  KRKVACMIGYCGTGYHGMQLNPPQK---TIEGDIFQAFVKAGAISQNNADDPKKSAFMRA 143

Query: 130 SRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDP 189
           +RTDKGVH+    ISLKM I +           +   IN +LP+ ++V+G+  + ++F+ 
Sbjct: 144 ARTDKGVHAAGNVISLKMIIEDEN---------IVEKINSHLPEQLRVWGVSRTNKAFEC 203

Query: 190 RRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHNYTIRSKYRK 249
           R+ C  R Y YL+P     +  +  T   +  + +         G  P        KY +
Sbjct: 204 RKLCSSRVYEYLMPTYSF-LNPRPGTVMSEKLLKD---------GTSPDEE---GKKYWE 263

Query: 250 TIRA-RQSPGYGHN-----------SGEEVSPSGMSNSGFEESDSEDNFFVEGVKG-PNQ 309
           ++ A  +S G  ++            GEE     ++ S  +     D   +E +K    +
Sbjct: 264 SVAADLESQGVSYDEWMKRACIDEIKGEETKE--VAESEVKTDSKTDAATLEKIKAVERR 323

Query: 310 HAPRLSVCCEDPVEV--CSKLGNGS-ELQKGVRARWLHEPDEKDRIGASHFRKILCCSCG 369
           H     +  E   ++    K+  G+         +   +P       A    K L CS  
Sbjct: 324 HREEFRISGERLAKIREILKIYEGTHNFHNFTLGKAFKDP------SAMRTMKSLTCSDP 383

Query: 370 KLETSLGYDFVEISIWGESFMLHQIRKMVG-TAVAVKRKLLPRDIIALSLTKFSRIVLPI 421
            L    G ++V I I G+SFMLHQIRKM+   A++V+    P+ +I  +  K +RI +P 
Sbjct: 384 FLID--GTEWVSIKIHGQSFMLHQIRKMISMVALSVRCNADPQKLIPQTFEK-ARINIPK 415

[Close]

BLAST of MC03g0025 vs. ExPASy Swiss-Prot
Match: Q4KM92 (tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 5.3e-21
Identity = 86/359 (23.96%), Postives = 149/359 (41.50%), Query Frame = 0


Query: 70  KKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARS 129
           K+K+V+ + Y+G  Y G+Q         TIE +L +A+ +AG I +++  ++ K+S+ R 
Sbjct: 79  KRKIVLLMAYSGKGYHGMQRNLGSSQFRTIEDDLVSALVQAGCIPENHGTDMRKMSFQRC 138

Query: 130 SRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDP 189
           +RTDKGV +    +SLK+      W+ D     + + IN +LP +I++ G+      F+ 
Sbjct: 139 ARTDKGVSAAGQVVSLKV------WLIDD----ILDKINSHLPSHIRILGLKRVTGGFNS 198

Query: 190 RRECDVRKYSYLLPAKLIGIESKFNTTEIDHHIS-----EFKDILNSFQGEHPFHNYTIR 249
           + +CD R Y Y+LP      + + +  +  + +S     +   +L  ++G H FHN+T +
Sbjct: 199 KNKCDARTYCYMLPTFAFAHKDR-DVQDESYRLSAETLQQVNRLLGCYKGTHNFHNFTSQ 258

Query: 250 SKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR---L 309
                                                          KGP + + R   L
Sbjct: 259 -----------------------------------------------KGPREPSARRYIL 318

Query: 310 SVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLGY 369
            + CE+P                    ++ E                           G 
Sbjct: 319 EMYCEEP--------------------FVRE---------------------------GL 331

Query: 370 DFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 421
           +F  I + G+SFM+HQIRKMVG  VA+ +   P  ++  S  +  ++ +P AP   LVL
Sbjct: 379 EFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGE-EKVDVPKAPGLGLVL 331

[Close]

BLAST of MC03g0025 vs. NCBI nr
Match: XP_022154871.1 (putative tRNA pseudouridine synthase isoform X1 [Momordica charantia])

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60
           MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD
Sbjct: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60

Query: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120
           QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN
Sbjct: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120

Query: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180
           LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI
Sbjct: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180

Query: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240
           VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN
Sbjct: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240

Query: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300
           YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR
Sbjct: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300

Query: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360
           LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG
Sbjct: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360

Query: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420
           YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL
Sbjct: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420

Query: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480
           RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE
Sbjct: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480

Query: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI 526
           KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI
Sbjct: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI 526

[Close]

BLAST of MC03g0025 vs. NCBI nr
Match: XP_022154872.1 (putative tRNA pseudouridine synthase isoform X2 [Momordica charantia])

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60
           MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD
Sbjct: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60

Query: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120
           QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN
Sbjct: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120

Query: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180
           LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI
Sbjct: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180

Query: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240
           VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN
Sbjct: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240

Query: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300
           YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR
Sbjct: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300

Query: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360
           LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG
Sbjct: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360

Query: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420
           YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL
Sbjct: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420

Query: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480
           RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE
Sbjct: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480

Query: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHK 514
           KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHK
Sbjct: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHK 514

[Close]

BLAST of MC03g0025 vs. NCBI nr
Match: XP_022970646.1 (putative tRNA pseudouridine synthase [Cucurbita maxima])

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 435/505 (86.14%), Postives = 462/505 (91.49%), Query Frame = 0


Query: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
           MAVSSLRFPLSPW TKTS  CFR PY  FVS +  LRPRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEG-VKGPNQHA 300
           NYTIRSKYR  IRA QSPGY H SG+ VS S +SNS FEESD E++ FV G +K PNQH+
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PRLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 360
           P LS C E+  EVCSKLGNGSEL +GVRARWLHEPDE+DRIGASHFRKILCCSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 LGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVL 420
           LGYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEW 480
           VLRGN FSN KRPGAPKRPDLLTLNKSDDI+KSV+EFYRSAILPE+TNFLD E+ PW EW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDKYTSIPNEQLDEVRNAWKAW 503
           VEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAW 505

[Close]

BLAST of MC03g0025 vs. NCBI nr
Match: XP_023520301.1 (putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 864 bits (2233), Expect = 2.81e-313
Identity = 429/504 (85.12%), Postives = 455/504 (90.28%), Query Frame = 0


Query: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
           MAVSSLRFPLSPW TKTS  CFR PY  FV  +  LRPRLLCC SS +ASTDA ST+CAP
Sbjct: 18  MAVSSLRFPLSPWATKTSFPCFRFPYPNFVLWTSRLRPRLLCCSSSSTASTDALSTQCAP 77

Query: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 78  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 137

Query: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NIKVFG
Sbjct: 138 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 197

Query: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 198 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 257

Query: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300
           NYTIRSKYR  IRA QSPGY H SG+ VS S  SNS FEESD E++ FV G+K PNQH+P
Sbjct: 258 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDFSNSEFEESDGENDIFVGGIKEPNQHSP 317

Query: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360
            LS   E+  EVCSKLGNGSEL +GVRARWLHEPDEKDRIGA HFRKIL CSCGKLETS 
Sbjct: 318 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGAPHFRKILYCSCGKLETSR 377

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSLTKFSRIV+PIAPAEVLV
Sbjct: 378 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLTKFSRIVMPIAPAEVLV 437

Query: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480
           LRGN FSN KRPGAPKRPDLLTLN+S DI+KSV+EFYRSAILPE+TNFLD E+ PW EWV
Sbjct: 438 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 497

Query: 481 EKLDKYTSIPNEQLDEVRNAWKAW 503
           EKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 498 EKLDKYTSIPNEQLEEVRNAWKAW 521

[Close]

BLAST of MC03g0025 vs. NCBI nr
Match: XP_038894774.1 (putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida])

HSP 1 Score: 862 bits (2228), Expect = 6.84e-313
Identity = 424/504 (84.13%), Postives = 460/504 (91.27%), Query Frame = 0


Query: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
           MAVSSLR PLSPW TKTSS+CF  PY  FVSR+  L P L  C +S +ASTDA ST+CAP
Sbjct: 1   MAVSSLRLPLSPWITKTSSSCFLFPYPTFVSRTHRLGPTLSGCSASSTASTDAFSTQCAP 60

Query: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
           +QLR WEAFRKKKVV+RIGY GTDYRGLQMQREQQSHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  NQLRKWEAFRKKKVVLRIGYVGTDYRGLQMQREQQSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LANYINFYLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTIALKMEIPESAWMGDPCGISLANYINFYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
           IVPSQ+SFDPR+ECDVRKYSY+LPAKLIGI +K  TTE+D+HIS+FKDILNSFQGEHPFH
Sbjct: 181 IVPSQKSFDPRKECDVRKYSYVLPAKLIGIGNKLKTTEVDYHISDFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300
           NYTIRSKYR  IRA++SPGYGH    EVSPS +SNS FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSKYRTRIRAKRSPGYGH----EVSPSDVSNSEFEESDGEDDTFVEGVKEPDQGSP 300

Query: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360
            LSVC E+P EVCSKLG+GSEL K VRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 
Sbjct: 301 SLSVCYENPTEVCSKLGDGSELPKDVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKM+GTA+AVKRKLLPRD+IALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMIGTAIAVKRKLLPRDVIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480
           LRGNSFSN KRPG PKRPDLLTLN+SDDI+KSV+EFYRSAILPE+TNFLDTE+ PW EWV
Sbjct: 421 LRGNSFSNRKRPGVPKRPDLLTLNESDDIVKSVNEFYRSAILPEVTNFLDTERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLDEVRNAWKAW 503
           EKLDKYTSIP+EQLDEVRNAWKAW
Sbjct: 481 EKLDKYTSIPSEQLDEVRNAWKAW 500

[Close]

BLAST of MC03g0025 vs. ExPASy TrEMBL
Match: A0A6J1DQ07 (putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 1075 bits (2781), Expect = 0.0
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60
           MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD
Sbjct: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60

Query: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120
           QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN
Sbjct: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120

Query: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180
           LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI
Sbjct: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180

Query: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240
           VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN
Sbjct: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240

Query: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300
           YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR
Sbjct: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300

Query: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360
           LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG
Sbjct: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360

Query: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420
           YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL
Sbjct: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420

Query: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480
           RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE
Sbjct: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480

Query: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI 526
           KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI
Sbjct: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHKRYIGHEEFKAPI 526

[Close]

BLAST of MC03g0025 vs. ExPASy TrEMBL
Match: A0A6J1DMV6 (putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022025 PE=3 SV=1)

HSP 1 Score: 1050 bits (2714), Expect = 0.0
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60
           MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD
Sbjct: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60

Query: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120
           QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN
Sbjct: 61  QLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGN 120

Query: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180
           LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI
Sbjct: 121 LYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGI 180

Query: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240
           VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN
Sbjct: 181 VPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFHN 240

Query: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300
           YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR
Sbjct: 241 YTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPR 300

Query: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360
           LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG
Sbjct: 301 LSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSLG 360

Query: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420
           YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL
Sbjct: 361 YDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLVL 420

Query: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480
           RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE
Sbjct: 421 RGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWVE 480

Query: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHK 514
           KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHK
Sbjct: 481 KLDKYTSIPNEQLDEVRNAWKAWKEKFESGQTHK 514

[Close]

BLAST of MC03g0025 vs. ExPASy TrEMBL
Match: A0A6J1I156 (putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569 PE=3 SV=1)

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 435/505 (86.14%), Postives = 462/505 (91.49%), Query Frame = 0


Query: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
           MAVSSLRFPLSPW TKTS  CFR PY  FVS +  LRPRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPWATKTSFPCFRFPYPNFVSWTSRLRPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEG-VKGPNQHA 300
           NYTIRSKYR  IRA QSPGY H SG+ VS S +SNS FEESD E++ FV G +K PNQH+
Sbjct: 241 NYTIRSKYRTRIRANQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGGIKEPNQHS 300

Query: 301 PRLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETS 360
           P LS C E+  EVCSKLGNGSEL +GVRARWLHEPDE+DRIGASHFRKILCCSCGKLETS
Sbjct: 301 PSLSGCNENTFEVCSKLGNGSELPQGVRARWLHEPDERDRIGASHFRKILCCSCGKLETS 360

Query: 361 LGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVL 420
           LGYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVL
Sbjct: 361 LGYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVL 420

Query: 421 VLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEW 480
           VLRGN FSN KRPGAPKRPDLLTLNKSDDI+KSV+EFYRSAILPE+TNFLD E+ PW EW
Sbjct: 421 VLRGNRFSNRKRPGAPKRPDLLTLNKSDDIVKSVNEFYRSAILPEVTNFLDIERPPWKEW 480

Query: 481 VEKLDKYTSIPNEQLDEVRNAWKAW 503
           VEKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 VEKLDKYTSIPNEQLEEVRNAWKAW 505

[Close]

BLAST of MC03g0025 vs. ExPASy TrEMBL
Match: A0A6J1HM83 (putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC111464888 PE=3 SV=1)

HSP 1 Score: 862 bits (2227), Expect = 5.89e-313
Identity = 427/504 (84.72%), Postives = 456/504 (90.48%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWT-KTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
           MAVSSLRFPLSPW  KTS  CFR PY  FVS +  L PRLLCC SS +ASTDA ST+CAP
Sbjct: 1   MAVSSLRFPLSPWANKTSFPCFRFPYPNFVSWTSRLSPRLLCCSSSSTASTDALSTQCAP 60

Query: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
           DQLR WEAFRKKKVVMRIGY GTDYRGLQMQREQ SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRKWEAFRKKKVVMRIGYVGTDYRGLQMQREQSSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
           +L KISWARSSRTDKGVHSLATTI+LKMEIP++AWMGDPCGILLANYINFYLP+NI+VFG
Sbjct: 121 DLNKISWARSSRTDKGVHSLATTITLKMEIPDSAWMGDPCGILLANYINFYLPENIRVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPRRECDVRKY+YLLPAKLIGIESK  TTEID+HISEFKDILNSFQGEHPFH
Sbjct: 181 IVPSQRSFDPRRECDVRKYTYLLPAKLIGIESKLTTTEIDYHISEFKDILNSFQGEHPFH 240

Query: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300
           NYTIRSKYR  IR  QSPGY H SG+ VS S +SNS FEESD E++ FV G+K PNQH+P
Sbjct: 241 NYTIRSKYRTRIRTNQSPGYVHKSGDGVSSSDLSNSEFEESDGENDIFVGGIKEPNQHSP 300

Query: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360
            LS   E+  EVCSKLGNGSEL +GVRARWLHEPDEKDRIGASHFRKIL CSCGKLETSL
Sbjct: 301 SLSGSYENTFEVCSKLGNGSELPQGVRARWLHEPDEKDRIGASHFRKILYCSCGKLETSL 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDFVEISIWGESFMLHQIRKMVGTA+AVKRKLLPRDIIALSL+KFSRIVLPIAPAEVLV
Sbjct: 361 GYDFVEISIWGESFMLHQIRKMVGTAIAVKRKLLPRDIIALSLSKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480
           LRGN FSN KRPGAPKRPDLLTLN+S DI+KSV+EFYRSAILPE+TNFLD E+ PW EWV
Sbjct: 421 LRGNRFSNRKRPGAPKRPDLLTLNESHDIVKSVNEFYRSAILPEVTNFLDIERPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLDEVRNAWKAW 503
           EKLDKYTSIPNEQL+EVRNAWKAW
Sbjct: 481 EKLDKYTSIPNEQLEEVRNAWKAW 504

[Close]

BLAST of MC03g0025 vs. ExPASy TrEMBL
Match: A0A1S3C5W1 (putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=3 SV=1)

HSP 1 Score: 845 bits (2182), Expect = 3.75e-306
Identity = 420/505 (83.17%), Postives = 453/505 (89.70%), Query Frame = 0


Query: 1   MAVSSLRFPLSPW-TKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAP 60
           M+VSSLR PLSPW TK+SS+CFR PY  FV R+P LRP LL C SS +ASTDA ST+CAP
Sbjct: 1   MSVSSLRLPLSPWITKSSSSCFRFPYPVFVPRTPRLRPTLLYCFSSAAASTDAFSTQCAP 60

Query: 61  DQLRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYG 120
           DQLR W+AFRKKKVV+RIGY GTDYRGLQMQR Q SHPTIEQELEAAI+KAGGIR+SNYG
Sbjct: 61  DQLRKWQAFRKKKVVLRIGYVGTDYRGLQMQRNQLSHPTIEQELEAAIYKAGGIRESNYG 120

Query: 121 NLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFG 180
           NL KISWARSSRTDKGVHSLATTI+LKMEIPE+AWMGDPCGI LA+YIN YLPDNIKVFG
Sbjct: 121 NLNKISWARSSRTDKGVHSLATTITLKMEIPESAWMGDPCGISLADYINSYLPDNIKVFG 180

Query: 181 IVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGEHPFH 240
           IVPSQRSFDPR+ECDVRKYSYLLPAKLIGIESK  TTEID HIS+FKDILNSF+GEHPFH
Sbjct: 181 IVPSQRSFDPRKECDVRKYSYLLPAKLIGIESKLKTTEIDCHISDFKDILNSFEGEHPFH 240

Query: 241 NYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAP 300
           NYTIRS YR  IRA+QSPGY   SG +VSP  MSN  FEESD ED+ FVEGVK P+Q +P
Sbjct: 241 NYTIRSNYRTRIRAKQSPGYALKSGNKVSPGEMSNFEFEESDREDDTFVEGVKEPDQCSP 300

Query: 301 RLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKLETSL 360
            LSVC E+  EVCSKL +GSEL KGVRARWLHEPDEKDRIGASHFR+ILCCSCGKLETS 
Sbjct: 301 SLSVC-ENLTEVCSKLEDGSELLKGVRARWLHEPDEKDRIGASHFRRILCCSCGKLETSF 360

Query: 361 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 420
           GYDF+EISIWGESFMLHQIRKM+GTAVAVKRKLLPRD+IALSLTKFSRIVLPIAPAEVLV
Sbjct: 361 GYDFIEISIWGESFMLHQIRKMIGTAVAVKRKLLPRDVIALSLTKFSRIVLPIAPAEVLV 420

Query: 421 LRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPWNEWV 480
           LRGNSFS  KRPG P RPDL+TLN+SDDIIKSV+EFYRSAILPE+T FLDTE  PW EWV
Sbjct: 421 LRGNSFSTRKRPGVPTRPDLVTLNESDDIIKSVNEFYRSAILPEVTEFLDTESPPWKEWV 480

Query: 481 EKLDKYTSIPNEQLDEVRNAWKAWK 504
           EKLDKYTSIPNE+LDEVR+AWKAWK
Sbjct: 481 EKLDKYTSIPNEELDEVRSAWKAWK 504

[Close]

BLAST of MC03g0025 vs. TAIR 10
Match: AT2G30320.1 (Pseudouridine synthase family protein )

HSP 1 Score: 495.7 bits (1275), Expect = 4.5e-140
Identity = 264/509 (51.87%), Postives = 342/509 (67.19%), Query Frame = 0


Query: 1   MAVSSLRFPLSPWTKTSSTCFRLPYIAFVSRSPHLRPRLLCCCSSPSASTDASSTRCAPD 60
           MAVS LR P+       S  F  P     +R   + P LL    S SA+   SST  +P 
Sbjct: 1   MAVSFLRIPI------PSRVFWYP-----TRLIRVTPALL--RFSSSAAFQPSSTSLSPP 60

Query: 61  Q-----LRTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRD 120
                    WE++RKKKVV+RIGY GTDYRGLQ+QR+  S  TIE ELE AI+KAGGIRD
Sbjct: 61  SSDNFLADKWESYRKKKVVIRIGYVGTDYRGLQIQRDDPSIKTIEGELEVAIYKAGGIRD 120

Query: 121 SNYGNLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNI 180
           SNYG+L+KI WARSSRTDKGVHSLAT+ISLKMEIPE AW  DP G +LA  I+ +LP+NI
Sbjct: 121 SNYGDLHKIGWARSSRTDKGVHSLATSISLKMEIPETAWKDDPQGTVLAKCISKHLPENI 180

Query: 181 KVFGIVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQGE 240
           +VF ++PS R FDPRREC +RKYSYLLP  ++GI++ F + EID+HI++F +IL  F+GE
Sbjct: 181 RVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLGIKNSFTSDEIDYHITDFNEILKEFEGE 240

Query: 241 HPFHNYTIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPN 300
           +PFHNYT RS+YR+    +     G    E  S             SE  F  E      
Sbjct: 241 YPFHNYTQRSRYRRKSEQKIKQRNGRPPREPKSIKA----------SESEFREENHIEIG 300

Query: 301 QHAPRLSVCCEDPVEVCSKLGNGSELQKGVRARWLHEPDEKDRIGASHFRKILCCSCGKL 360
           +      V  E    V +   + S++    RA+WL+EPDE D+I  +HFRK+  C  GKL
Sbjct: 301 EEEEEKEVDGESDEHVVTPDSDNSQVYS--RAKWLYEPDETDKISGAHFRKVFRCRSGKL 360

Query: 361 ETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPA 420
           E SLG+ FVEISIWGESFMLHQIRKM+GTAVAVKR+LLPRDII LSL KF+RIVLP+AP+
Sbjct: 361 ENSLGFGFVEISIWGESFMLHQIRKMIGTAVAVKRELLPRDIIRLSLNKFTRIVLPLAPS 420

Query: 421 EVLVLRGNSFSNGKRPGAPKRPDLLTLNKSDDIIKSVDEFYRSAILPELTNFLDTEKAPW 480
           EVL+LRGNSF   +    P+RP +    +S+++ K ++EFYR+ ++P+++ FLD+EK+PW
Sbjct: 421 EVLILRGNSFEVRR---LPERPGMEATGESEEVEKEIEEFYRAVMVPQVSIFLDSEKSPW 480

Query: 481 NEWVEKLDKYTSIPNEQLDEVRNAWKAWK 505
            EWV+ LD+   + +E+L++VR  W+ WK
Sbjct: 481 KEWVDHLDRNDGLIDEELEDVRKGWEEWK 481

[Close]

BLAST of MC03g0025 vs. TAIR 10
Match: AT1G20370.1 (Pseudouridine synthase family protein )

HSP 1 Score: 117.5 bits (293), Expect = 3.3e-26
Identity = 114/421 (27.08%), Postives = 180/421 (42.76%), Query Frame = 0


Query: 44  SSPSASTDASSTRCAPDQL--RTWEAFRKKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQ 103
           S+    TD+S+       +  R    +R++KV +   + G  Y+G+Q   +     TIE 
Sbjct: 29  STTGQETDSSAVTTGGSNVAERRAPKYRRRKVAIVFAFCGVGYQGMQ---KNPGAKTIEG 88

Query: 104 ELEAAIFKAGGIRDSNYGNLYKISWARSSRTDKGVHSLATTISLKMEIPENAWMGDPCGI 163
           ELE A+F AG + D++        WARS+RTDKGV ++   +S +  +       DP G 
Sbjct: 89  ELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVGQVVSGRFYV-------DPPGF 148

Query: 164 LLANYINFYLPDNIKVFGIVPSQRSFDPRRECDVRKYSYLLP-----------AKLIGIE 223
           +    +N  LPD I+VFG      SF  ++ CD R+Y YL+P           A+++  +
Sbjct: 149 V--ERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALDPCVHGEAEMVRTD 208

Query: 224 SKFNTTEIDHHISEFKDILNSFQGEHPFHNYTIRSKYRKTIRARQSPGYGHNSGEEV--- 283
             +   +      +   I     G+      T   + +  I +      G +   E    
Sbjct: 209 LGYEYVKCVECSEKGYKIPLGVMGKDTDCCDTKSLEIQSDISSSTCDAIGADVKCETLSS 268

Query: 284 -SPSGMSNSGFEESDSED----NFFVEGVKGPNQHAPRLSVCCEDPVEVCSKLGNGSELQ 343
             PS  +N   E  D  D    +   EG++  N  A           +V    G G ++ 
Sbjct: 269 SVPSAENNLNSEVLDGADVSALSSKAEGMEESNTLA-----------KVEMNNGEGEDMT 328

Query: 344 KGVRARWLHEPDEKDR--------IGASHF---------------RKILCCSCGKLETSL 403
           K   +++ +   EK+R        +G+ +F               R IL  +   +    
Sbjct: 329 KS-ESKFCYGEKEKERFNRILSYYVGSHNFHNFTTRTKAADPAANRYILSFNANTVINLD 388

Query: 404 GYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIALSLTKFSRIVLPIAPAEVLV 421
           G DFV+  + G+SFMLHQIRKM+G AVA+ R   P  +I  S  K  RI +P+AP   L 
Sbjct: 389 GKDFVKCEVVGQSFMLHQIRKMIGLAVAIMRNYAPESLIEASFKKDVRINVPMAPEVGLY 425

[Close]

BLAST of MC03g0025 vs. TAIR 10
Match: AT1G76120.1 (Pseudouridine synthase family protein )

HSP 1 Score: 116.7 bits (291), Expect = 5.6e-26
Identity = 109/391 (27.88%), Postives = 170/391 (43.48%), Query Frame = 0


Query: 71  KKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARSS 130
           +KV +   + G  Y+G+Q   +     TIE ELE A+F AG + +S  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 131 RTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDPR 190
           RTDKGV ++   +S         ++ DP G +  N +N  LP+ I++FG      SF  +
Sbjct: 82  RTDKGVSAVGQVVS-------GRFIVDPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 191 RECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSF-QGEHPFHNYTIRSKYRK 250
           + CD R+Y YLLP         F    I H   E   ++ S   GE     +    + RK
Sbjct: 142 KFCDRRRYVYLLPV--------FALDPISHRDRE--TVMASLGPGEEYVKCFECSERGRK 201

Query: 251 TIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPRLSVCCEDPV 310
                     G N G +        S    S    +  +E +      +  L+  C   V
Sbjct: 202 IPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEAL------SSNLAGLCSVDV 261

Query: 311 EV-------CSKLGNGSELQKGVRARWLHEPDEKDR--------IGASHF---------- 370
           EV       C    N SE +  V++++ +   EK+R        +G+ +F          
Sbjct: 262 EVGRIQEDSCKLNTNSSETK--VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKAD 321

Query: 371 -----RKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIA 430
                R+I+  +   +    G DF++  + G+SFMLHQIRKM+G AVA+ R      +I 
Sbjct: 322 DPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQ 381

[Close]

BLAST of MC03g0025 vs. TAIR 10
Match: AT1G76120.2 (Pseudouridine synthase family protein )

HSP 1 Score: 108.6 bits (270), Expect = 1.5e-23
Identity = 101/365 (27.67%), Postives = 158/365 (43.29%), Query Frame = 0


Query: 71  KKVVMRIGYAGTDYRGLQMQREQQSHPTIEQELEAAIFKAGGIRDSNYGNLYKISWARSS 130
           +KV +   + G  Y+G+Q   +     TIE ELE A+F AG + +S  G      +ARS+
Sbjct: 22  QKVAIIFAFCGVGYQGMQ---KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSA 81

Query: 131 RTDKGVHSLATTISLKMEIPENAWMGDPCGILLANYINFYLPDNIKVFGIVPSQRSFDPR 190
           RTDKGV ++   +S         ++ DP G +  N +N  LP+ I++FG      SF  +
Sbjct: 82  RTDKGVSAVGQVVS-------GRFIVDPLGFV--NRLNSNLPNQIRIFGYKHVTPSFSSK 141

Query: 191 RECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSF-QGEHPFHNYTIRSKYRK 250
           + CD R+Y YLLP         F    I H   E   ++ S   GE     +    + RK
Sbjct: 142 KFCDRRRYVYLLPV--------FALDPISHRDRE--TVMASLGPGEEYVKCFECSERGRK 201

Query: 251 TIRARQSPGYGHNSGEEVSPSGMSNSGFEESDSEDNFFVEGVKGPNQHAPRLSVCCEDPV 310
                     G N G +        S    S    +  +E +      +  L+  C   V
Sbjct: 202 IPPGLVGKWKGTNFGTKSLDFQSDISSNNSSALRSDIKIEAL------SSNLAGLCSVDV 261

Query: 311 EV-------CSKLGNGSELQKGVRARWLHEPDEKDR--------IGASHF---------- 370
           EV       C    N SE +  V++++ +   EK+R        +G+ +F          
Sbjct: 262 EVGRIQEDSCKLNTNSSETK--VKSKFCYGEKEKERFSRILSCYVGSYNFHNFTTRTKAD 321

Query: 371 -----RKILCCSCGKLETSLGYDFVEISIWGESFMLHQIRKMVGTAVAVKRKLLPRDIIA 405
                R+I+  +   +    G DF++  + G+SFMLHQIRKM+G AVA+ R      +I 
Sbjct: 322 DPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMMGLAVAIMRNCASESLIQ 356

[Close]

BLAST of MC03g0025 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 54.3 bits (129), Expect = 3.4e-07
Identity = 59/229 (25.76%), Postives = 87/229 (37.99%), Query Frame = 0


Query: 70  KKKVVMRIGYAGTDYRGLQMQREQQSHPTIEQE----LEAAIFKAGGIRDSNYGNLYKIS 129
           K+ V ++I Y G  + G     E Q  P+IE E    LE      G  +DS Y       
Sbjct: 89  KRYVALKIMYFGKRFYG--FSAEAQMEPSIESEVFKALERTRLLVGDKKDSCY------- 148

Query: 130 WARSSRTDKGVHSLATTISL----KMEIPE-------NAWMGDPCGILLANYINFYLPDN 189
            +R  RTDKGV S    I+L    +++ P        N   G+         +N  LPD+
Sbjct: 149 -SRCGRTDKGVSSTGQVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDD 208

Query: 190 IKVFGIVPSQRSFDPRRECDVRKYSYLLPAKLIGIESKFNTTEIDHHISEFKDILNSFQG 249
           I+V G  P+   F  R  C  R+Y Y    + +             ++S        F G
Sbjct: 209 IRVIGWSPAPIDFHARFSCYAREYKYFFWRQNL-------------NLSAMDFAGKKFIG 268

Query: 250 EHPFHNY------TIRSKYRKTIRARQSPGYGHNSGEEVSPSGMSNSGF 278
           EH F N+       +    R+      SP    + G+++    M  S F
Sbjct: 269 EHDFRNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAF 294

The following BLAST results are available for this feature:

BLAST of MC03g0025 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22928	6.3e-139	51.87	Putative tRNA pseudouridine synthase OS=Arabidopsis thaliana OX=3702 GN=At2g3032...	[more]
Q6CWQ8	6.7e-24	30.06	tRNA pseudouridine synthase 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 23...	[more]
Q59S63	1.8e-21	29.68	tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876...	[more]
Q6CC39	2.4e-21	28.26	tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) O...	[more]
Q4KM92	5.3e-21	23.96	tRNA pseudouridine synthase A OS=Rattus norvegicus OX=10116 GN=Pus1 PE=1 SV=2	[more]

BLAST of MC03g0025 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154871.1	0.0	100.00	putative tRNA pseudouridine synthase isoform X1 [Momordica charantia]	[more]
XP_022154872.1	0.0	100.00	putative tRNA pseudouridine synthase isoform X2 [Momordica charantia]	[more]
XP_022970646.1	0.0	86.14	putative tRNA pseudouridine synthase [Cucurbita maxima]	[more]
XP_023520301.1	2.81e-313	85.12	putative tRNA pseudouridine synthase [Cucurbita pepo subsp. pepo]	[more]
XP_038894774.1	6.84e-313	84.13	putative tRNA pseudouridine synthase isoform X1 [Benincasa hispida]	[more]

BLAST of MC03g0025 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DQ07	0.0	100.00	putative tRNA pseudouridine synthase isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DMV6	0.0	100.00	putative tRNA pseudouridine synthase isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1I156	0.0	86.14	putative tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111469569...	[more]
A0A6J1HM83	5.89e-313	84.72	putative tRNA pseudouridine synthase OS=Cucurbita moschata OX=3662 GN=LOC1114648...	[more]
A0A1S3C5W1	3.75e-306	83.17	putative tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497029 PE=...	[more]

BLAST of MC03g0025 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30320.1	4.5e-140	51.87	Pseudouridine synthase family protein	[more]
AT1G20370.1	3.3e-26	27.08	Pseudouridine synthase family protein	[more]
AT1G76120.1	5.6e-26	27.88	Pseudouridine synthase family protein	[more]
AT1G76120.2	1.5e-23	27.67	Pseudouridine synthase family protein	[more]
AT1G34150.1	3.4e-07	25.76	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020097	Pseudouridine synthase I, TruA, alpha/beta domain	PFAM	PF01416	PseudoU_synth_1	coord: 340..420 e-value: 8.1E-7 score: 29.5
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 194..493 e-value: 2.5E-34 score: 120.5
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 62..193 e-value: 1.0E-30 score: 108.3
None	No IPR available	PIRSR	PIRSR001430-2	PIRSR001430-2	coord: 124..252 e-value: 4.4E-16 score: 57.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 254..281
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 261..281
None	No IPR available	PANTHER	PTHR11142:SF21	TRNA PSEUDOURIDINE SYNTHASE	coord: 47..508
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 47..508
IPR041708	Pseudouridine synthase PUS1/ PUS2-like	CDD	cd02568	PseudoU_synth_PUS1_PUS2	coord: 75..425 e-value: 5.83615E-79 score: 245.601
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 341..427
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 70..245

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC03g0025.1	MC03g0025.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990481	mRNA pseudouridine synthesis
biological_process	GO:0031119	tRNA pseudouridine synthesis
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
cellular_component	GO:0005634	nucleus
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding