MC02g_new0273 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC02g_new0273
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Reverse transcriptase
Location	MC02: 11943816 .. 11944693 (+)
RNA-Seq Expression	MC02g_new0273
Synteny	MC02g_new0273

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGACCATACTTATGTGGGAAAGAGTCCTCAAGCTTACAACTTCACCAAGGAATGGTGTCAGTCCATTGAAGTGGCCCAAGCATACCTCGAGAAGGCATCTAAACACATGAAAAAGTGGGCAGACAAAAAGAGAAGACCTTTAGAATTCCAAGCAGGATATCAAGTCCTTGTGAAGTTGAGACCTGAACAACTTCGTTTCCAGGGTAACAAAGATCAACGCCTTGTGCGAAAATACGAAGGACCCGTCAACGTCCTTTAGAAGGTGGGCAAGACATCCTATAGGGTACAACTACCCTTGTGGATGAAAATTCATCCAGTGATCCACGTGAGCAACCTGAAGCCTTACCATCAAGACACCGTTGACCCTACACGAAATGAGATAACTCGTCCAGCAATCATGTTGACAAACAAGGTTGACAAAGAAGTTGAGTCAATCCTCGCTGAAAGAACACGCCGAGTAGAAAGTCCACGAAGGGACATACAGGAATTCTTAGTCAAGTGGAAAGGGCTCCCAGATGAAGAGATCCGTTGGGAGCATACTGAAGACCTGAAGACAACTGTAGCAGCGATTGAAGAGTTCGAGCAGCGACGGTTGACAGGGACGTCAACTATTTAAGTGGGGGAGAATGTCATGGTCATGCTCGTCTAGAGTCTGTTGCCCGTGGCCGCATGCCCATAACAACCTTTTTCCACATACTTTTATGTTATGTATTTAGTTTCGCATGTTATTTTCTTTTCCTTGTATTTTTGGTGGTGAGTAAGTCCGCACTTTGCACGTTCATATCTGCCAAAGCAAATATGCCTTAGAATATACTATAGGGGGGTACACCTAGTCAGCCTCCCTACATTGCGTTGTATTTTTATCTTGCCGCTCAT

mRNA sequence

ATGAAAATTCATCCAGTGATCCACGTGAGCAACCTGAAGCCTTACCATCAAGACACCGTTGACCCTACACGAAATGAGATAACTCGTCCAGCAATCATGTTGACAAACAAGGTTGACAAAGAAGTTGAGTCAATCCTCGCTGAAAGAACACGCCGAGTAGAAAGTCCACGAAGGGACATACAGGAATTCTTAGTCAAGTGGAAAGGGCTCCCAGATGAAGAGATCCGTTGGGAGCATACTGAAGACCTGAAGACAACTGTAGCAGCGATTGAAGAGTTCGAGCAGCGACGGTTGACAGGGACGTCAACTATTTAA

Coding sequence (CDS)

ATGAAAATTCATCCAGTGATCCACGTGAGCAACCTGAAGCCTTACCATCAAGACACCGTTGACCCTACACGAAATGAGATAACTCGTCCAGCAATCATGTTGACAAACAAGGTTGACAAAGAAGTTGAGTCAATCCTCGCTGAAAGAACACGCCGAGTAGAAAGTCCACGAAGGGACATACAGGAATTCTTAGTCAAGTGGAAAGGGCTCCCAGATGAAGAGATCCGTTGGGAGCATACTGAAGACCTGAAGACAACTGTAGCAGCGATTGAAGAGTTCGAGCAGCGACGGTTGACAGGGACGTCAACTATTTAA

Protein sequence

MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI

Homology

[Close]

BLAST of MC02g_new0273 vs. NCBI nr
Match: XP_022148918.1 (uncharacterized protein LOC111017468 [Momordica charantia])

HSP 1 Score: 196 bits (497), Expect = 7.60e-60
Identity = 99/99 (100.00%), Postives = 99/99 (100.00%), Query Frame = 0


Query: 6   VIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLV 65
           VIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLV
Sbjct: 223 VIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLV 282

Query: 66  KWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
           KWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI
Sbjct: 283 KWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 321

[Close]

BLAST of MC02g_new0273 vs. NCBI nr
Match: TYK04336.1 (2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 125 bits (315), Expect = 5.09e-35
Identity = 66/104 (63.46%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+PVIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 1   MKIYPVIHVSNLKPYHQDTEDLQRNIVTRPIIDLSQKEDKDVEEILAERVRRGRRPTRRI 60

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     I++F+ R+LTGTSTI
Sbjct: 61  HEYLVKWKNLPVEETSWERVEDLEAWKQKIKDFKLRQLTGTSTI 104

[Close]

BLAST of MC02g_new0273 vs. NCBI nr
Match: TYK19637.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK20902.1 reverse transcriptase [Cucumis melo var. makuwa] >TYK28777.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 128 bits (321), Expect = 4.68e-34
Identity = 67/104 (64.42%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+PVIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 156 MKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPARRI 215

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     IE+F+ R+LTGTSTI
Sbjct: 216 HEYLVKWKNLPVEETSWERVEDLEAWKQKIEDFKLRQLTGTSTI 259

[Close]

BLAST of MC02g_new0273 vs. NCBI nr
Match: KAA0062339.1 (uncharacterized protein E6C27_scaffold154G00970 [Cucumis melo var. makuwa] >TYK26635.1 uncharacterized protein E5676_scaffold313G002810 [Cucumis melo var. makuwa])

HSP 1 Score: 124 bits (311), Expect = 6.03e-34
Identity = 66/104 (63.46%), Postives = 75/104 (72.12%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+ VIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 37  MKIYSVIHVSNLKPYHQDTEDLQRNIVTRPIIDLSQKEDKDVEEILAERVRRDRRPARRI 96

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     IE+F+ R+LTGTSTI
Sbjct: 97  HEYLVKWKNLPVEETSWERVEDLEAWKQKIEDFKLRQLTGTSTI 140

[Close]

BLAST of MC02g_new0273 vs. NCBI nr
Match: KAA0060349.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK22099.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 127 bits (318), Expect = 6.41e-34
Identity = 67/104 (64.42%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+PVIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 127 MKIYPVIHVSNLKPYHQDTEDLQRNIVTRPIIDLSQKEDKDVEEILAERVRRGRRPARRI 186

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     IE+F+ R+LTGTSTI
Sbjct: 187 HEYLVKWKNLPVEETSWERVEDLEAWKQKIEDFKFRQLTGTSTI 230

[Close]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL
Match: A0A6J1D5E5 (uncharacterized protein LOC111017468 OS=Momordica charantia OX=3673 GN=LOC111017468 PE=4 SV=1)

HSP 1 Score: 196 bits (497), Expect = 3.68e-60
Identity = 99/99 (100.00%), Postives = 99/99 (100.00%), Query Frame = 0


Query: 6   VIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLV 65
           VIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLV
Sbjct: 223 VIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDIQEFLV 282

Query: 66  KWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
           KWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI
Sbjct: 283 KWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 321

[Close]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL
Match: A0A5D3C1P4 (2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold45522G00010 PE=4 SV=1)

HSP 1 Score: 125 bits (315), Expect = 2.47e-35
Identity = 66/104 (63.46%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+PVIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 1   MKIYPVIHVSNLKPYHQDTEDLQRNIVTRPIIDLSQKEDKDVEEILAERVRRGRRPTRRI 60

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     I++F+ R+LTGTSTI
Sbjct: 61  HEYLVKWKNLPVEETSWERVEDLEAWKQKIKDFKLRQLTGTSTI 104

[Close]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL
Match: A0A5D3DBE0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00240 PE=4 SV=1)

HSP 1 Score: 128 bits (321), Expect = 2.27e-34
Identity = 67/104 (64.42%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+PVIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 156 MKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPARRI 215

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     IE+F+ R+LTGTSTI
Sbjct: 216 HEYLVKWKNLPVEETSWERVEDLEAWKQKIEDFKLRQLTGTSTI 259

[Close]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL
Match: A0A5D3DTG2 (Chromo domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G002810 PE=4 SV=1)

HSP 1 Score: 124 bits (311), Expect = 2.92e-34
Identity = 66/104 (63.46%), Postives = 75/104 (72.12%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+ VIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 37  MKIYSVIHVSNLKPYHQDTEDLQRNIVTRPIIDLSQKEDKDVEEILAERVRRDRRPARRI 96

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     IE+F+ R+LTGTSTI
Sbjct: 97  HEYLVKWKNLPVEETSWERVEDLEAWKQKIEDFKLRQLTGTSTI 140

[Close]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL
Match: A0A5A7V3K4 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold318G00880 PE=4 SV=1)

HSP 1 Score: 127 bits (318), Expect = 3.10e-34
Identity = 67/104 (64.42%), Postives = 76/104 (73.08%), Query Frame = 0


Query: 1   MKIHPVIHVSNLKPYHQDTVDPTRNEITRPAIMLTNKVDKEVESILAERTRRVESPRRDI 60
           MKI+PVIHVSNLKPYHQDT D  RN +TRP I L+ K DK+VE ILAER RR   P R I
Sbjct: 127 MKIYPVIHVSNLKPYHQDTEDLQRNIVTRPIIDLSQKEDKDVEEILAERVRRGRRPARRI 186

Query: 61  QEFLVKWKGLPDEEIRWEHTEDLKTTVAAIEEFEQRRLTGTSTI 104
            E+LVKWK LP EE  WE  EDL+     IE+F+ R+LTGTSTI
Sbjct: 187 HEYLVKWKNLPVEETSWERVEDLEAWKQKIEDFKFRQLTGTSTI 230

The following BLAST results are available for this feature:

BLAST of MC02g_new0273 vs.


Match Name	E-value	Identity	Description

BLAST of MC02g_new0273 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148918.1	7.60e-60	100.00	uncharacterized protein LOC111017468 [Momordica charantia]	[more]
TYK04336.1	5.09e-35	63.46	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Cucumis melo var. maku...	[more]
TYK19637.1	4.68e-34	64.42	reverse transcriptase [Cucumis melo var. makuwa] >TYK20902.1 reverse transcripta...	[more]
KAA0062339.1	6.03e-34	63.46	uncharacterized protein E6C27_scaffold154G00970 [Cucumis melo var. makuwa] >TYK2...	[more]
KAA0060349.1	6.41e-34	64.42	reverse transcriptase [Cucumis melo var. makuwa] >TYK22099.1 reverse transcripta...	[more]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5E5	3.68e-60	100.00	uncharacterized protein LOC111017468 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A5D3C1P4	2.47e-35	63.46	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Cucumis melo var. ma...	[more]
A0A5D3DBE0	2.27e-34	64.42	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold24...	[more]
A0A5D3DTG2	2.92e-34	63.46	Chromo domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7V3K4	3.10e-34	64.42	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold31...	[more]

BLAST of MC02g_new0273 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000953	Chromo/chromo shadow domain	SMART	SM00298	chromo_7	coord: 39..98 e-value: 1.1E-5 score: 34.9
IPR000953	Chromo/chromo shadow domain	PROSITE	PS50013	CHROMO_2	coord: 40..104 score: 13.896901
IPR023780	Chromo domain	PFAM	PF00385	Chromo	coord: 41..96 e-value: 4.7E-12 score: 45.6
None	No IPR available	GENE3D	2.40.50.40		coord: 40..101 e-value: 1.8E-13 score: 52.0
None	No IPR available	CDD	cd00024	CD_CSD	coord: 41..95 e-value: 7.82802E-11 score: 50.9385
IPR016197	Chromo-like domain superfamily	SUPERFAMILY	54160	Chromo domain-like	coord: 1..97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC02g_new0273.1	MC02g_new0273.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0016779	nucleotidyltransferase activity