MC02g_new0257 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g_new0257
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNB-ARC domain-containing protein
LocationMC02: 11464598 .. 11465638 (+)
RNA-Seq ExpressionMC02g_new0257
SyntenyMC02g_new0257
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TACAAGTGCAGGTCGTATTTTGTTGTCATTGTATGTTGATGATATGATCATTACTGGTGATGATTCTGATGGAATAGAATCTTTGAAGTCTGAGCTATCGAGTTGTTTTGCTATGAAAGACTTGGGTATGTTGCACTACTTTCTCGACATTGAGGTTGCTTATTCTCCAAAAGGTTATCTCTTATCTCAGTCAAAGTATAGAGTTGATTTGTTTGATCGTGTGCGTCTCACTGACAATAGGATCGTTGATACTCCCCTTGAGCCCAATGCCCGATATTCTCCGTTGGATGGGATTCCTTTGTCGGATTTCAGTTTATATCGTACTATTGTTGGGAGTTTGGTCTATCTTACTGTGACTTGTCTAGACATTGCACATGTTGTTCATGTGGTTAGTCAGTTTGTCACTGCTCCTGCTATAGTTCATTGGGTTGTCGTTCTTCGTATTCCCAGATATCTTAGAGGCACTCAATTTCAGAGTCTTTTGTTTCTTTCCACGTCATCTCTAGACTTGCGTGCTTACTCTGATGCTGATTGGGTTGGTGATCTTACTGATCGCAAGTCGACCACTGGATTCTGTATTTTCTTTGGTGATTCCCTTATTTCATGGAAGAGTAAGAAACACGATGTTATTTCTCGATCTTCTACAAAAGCTGAGTATCGTGTCATGGCTTCGACTACTTGTGAAATTGTTTGGTTACGTTGGCTACTTGCAGATATGGGCATTTATCTTCATCATCCTACTCCCTTATATTGTGACAATCAAAGTGCGATTCAGATTGCATGCAATTCGGTTTTTCATAAACGAACCAAGCATATTGAGATTGATTGTCATGTCACTCGTCATCATCTTCAACGGGGCTCTATCACATTACCTTTTGTTTCATCATCTCTACAGATTGTTGATATGTTTACGAAGTCACACTCCGCTTCGCCCTTTCGTTTTTTATCTGACAAACTCTCAATGCTTATTGCTGTAGCATCGTGAGTTTGCGGGGGATGTTAGCATAAATATTATAATTATTATTATTAGTCTTTGAGGGT

mRNA sequence

ATGATCATTACTGGTGATGATTCTGATGGAATAGAATCTTTGAAGTCTGAGCTATCGAGTTGTTTTGCTATGAAAGACTTGGGTATGTTGCACTACTTTCTCGACATTGAGGTTGCTTATTCTCCAAAAGGTTATCTCTTATCTCAGTCAAAGTATAGAGTTGATTTGTTTGATCGTGTGCGTCTCACTGACAATAGGATCGTTGATACTCCCCTTGAGCCCAATGCCCGATATTCTCCGTTGGATGGGATTCCTTTGTCGGATTTCAGTTTATATCGTACTATTGTTGGGAGTTTGGTCTATCTTACTGTGACTTGTCTAGACATTGCACATGTTGTTCATGTGGTTAGTCAGTTTGTCACTGCTCCTGCTATAGTTCATTGGGTTGTCGTTCTTCGTATTCCCAGATATCTTAGAGGCACTCAATTTCAGAGTCTTTTGTTTCTTTCCACGTCATCTCTAGACTTGCGTGCTTACTCTGATGCTGATTGGGTTGGTGATCTTACTGATCGCAAGTCGACCACTGGATTCTGTATTTTCTTTGGTGATTCCCTTATTTCATGGAAGAGTAAGAAACACGATGTTATTTCTCGATCTTCTACAAAAGCTGAGTATCGTGTCATGGCTTCGACTACTTGTGAAATTGTTTGGTTACGTTGGCTACTTGCAGATATGGGCATTTATCTTCATCATCCTACTCCCTTATATTGTGACAATCAAAGTGCGATTCAGATTGCATGCAATTCGGTTTTTCATAAACGAACCAAGCATATTGAGATTGATTGTCATGTCACTCGTCATCATCTTCAACGGGGCTCTATCACATTACCTTTTGTTTCATCATCTCTACAGATTGTTGATATGTTTACGAAGTCACACTCCGCTTCGCCCTTTCGTTTTTTATCTGACAAACTCTCAATGCTTATTGCTGTAGCATCGTGA

Coding sequence (CDS)

ATGATCATTACTGGTGATGATTCTGATGGAATAGAATCTTTGAAGTCTGAGCTATCGAGTTGTTTTGCTATGAAAGACTTGGGTATGTTGCACTACTTTCTCGACATTGAGGTTGCTTATTCTCCAAAAGGTTATCTCTTATCTCAGTCAAAGTATAGAGTTGATTTGTTTGATCGTGTGCGTCTCACTGACAATAGGATCGTTGATACTCCCCTTGAGCCCAATGCCCGATATTCTCCGTTGGATGGGATTCCTTTGTCGGATTTCAGTTTATATCGTACTATTGTTGGGAGTTTGGTCTATCTTACTGTGACTTGTCTAGACATTGCACATGTTGTTCATGTGGTTAGTCAGTTTGTCACTGCTCCTGCTATAGTTCATTGGGTTGTCGTTCTTCGTATTCCCAGATATCTTAGAGGCACTCAATTTCAGAGTCTTTTGTTTCTTTCCACGTCATCTCTAGACTTGCGTGCTTACTCTGATGCTGATTGGGTTGGTGATCTTACTGATCGCAAGTCGACCACTGGATTCTGTATTTTCTTTGGTGATTCCCTTATTTCATGGAAGAGTAAGAAACACGATGTTATTTCTCGATCTTCTACAAAAGCTGAGTATCGTGTCATGGCTTCGACTACTTGTGAAATTGTTTGGTTACGTTGGCTACTTGCAGATATGGGCATTTATCTTCATCATCCTACTCCCTTATATTGTGACAATCAAAGTGCGATTCAGATTGCATGCAATTCGGTTTTTCATAAACGAACCAAGCATATTGAGATTGATTGTCATGTCACTCGTCATCATCTTCAACGGGGCTCTATCACATTACCTTTTGTTTCATCATCTCTACAGATTGTTGATATGTTTACGAAGTCACACTCCGCTTCGCCCTTTCGTTTTTTATCTGACAAACTCTCAATGCTTATTGCTGTAGCATCGTGA

Protein sequence

MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRVRLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFVTAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIFFGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQSAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRFLSDKLSMLIAVAS
Homology
BLAST of MC02g_new0257 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 4.2e-58
Identity = 126/307 (41.04%), Postives = 177/307 (57.65%), Query Frame = 0

Query: 1    MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
            ++ITG+D   + +    LS  F++KD   LHYFL IE    P G  LSQ +Y +DL  R 
Sbjct: 1154 ILITGNDPTLLHNTLDNLSQRFSVKDHEELHYFLGIEAKRVPTGLHLSQRRYILDLLART 1213

Query: 61   RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
             +   + V TP+ P+ + S   G  L+D + YR IVGSL YL  T  DI++ V+ +SQF+
Sbjct: 1214 NMITAKPVTTPMAPSPKLSLYSGTKLTDPTEYRGIVGSLQYLAFTRPDISYAVNRLSQFM 1273

Query: 121  TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
              P   H   + RI RYL GT    +     ++L L AYSDADW GD  D  ST G+ ++
Sbjct: 1274 HMPTEEHLQALKRILRYLAGTPNHGIFLKKGNTLSLHAYSDADWAGDKDDYVSTNGYIVY 1333

Query: 181  FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
             G   ISW SKK   + RSST+AEYR +A+T+ E+ W+  LL ++GI L  P  +YCDN 
Sbjct: 1334 LGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEMQWICSLLTELGIRLTRPPVIYCDNV 1393

Query: 241  SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
             A  +  N VFH R KHI ID H  R+ +Q G++ +  VS+  Q+ D  TK  S + F+ 
Sbjct: 1394 GATYLCANPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRTAFQN 1453

Query: 301  LSDKLSM 308
             + K+ +
Sbjct: 1454 FASKIGV 1460

BLAST of MC02g_new0257 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 122/308 (39.61%), Postives = 179/308 (58.12%), Query Frame = 0

Query: 1    MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
            ++ITG+D+  ++     LS  F++K+   LHYFL IE    P+G  LSQ +Y +DL  R 
Sbjct: 1137 ILITGNDTVLLKHTLDALSQRFSVKEHEDLHYFLGIEAKRVPQGLHLSQRRYTLDLLART 1196

Query: 61   RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
             +   + V TP+  + + +   G  L D + YR IVGSL YL  T  D+++ V+ +SQ++
Sbjct: 1197 NMLTAKPVATPMATSPKLTLHSGTKLPDPTEYRGIVGSLQYLAFTRPDLSYAVNRLSQYM 1256

Query: 121  TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
              P   HW  + R+ RYL GT    +     ++L L AYSDADW GD  D  ST G+ ++
Sbjct: 1257 HMPTDDHWNALKRVLRYLAGTPDHGIFLKKGNTLSLHAYSDADWAGDTDDYVSTNGYIVY 1316

Query: 181  FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
             G   ISW SKK   + RSST+AEYR +A+T+ E+ W+  LL ++GI L HP  +YCDN 
Sbjct: 1317 LGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSELQWICSLLTELGIQLSHPPVIYCDNV 1376

Query: 241  SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
             A  +  N VFH R KHI +D H  R+ +Q G++ +  VS+  Q+ D  TK  S   F+ 
Sbjct: 1377 GATYLCANPVFHSRMKHIALDYHFIRNQVQSGALRVVHVSTHDQLADTLTKPLSRVAFQN 1436

Query: 301  LSDKLSML 309
             S K+ ++
Sbjct: 1437 FSRKIGVI 1444

BLAST of MC02g_new0257 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.6e-41
Identity = 112/316 (35.44%), Postives = 169/316 (53.48%), Query Frame = 0

Query: 1    MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVA--YSPKGYLLSQSKYRVDLFD 60
            M+I G D   I  LK +LS  F MKDLG     L +++    + +   LSQ KY   + +
Sbjct: 1012 MLIVGKDKGLIAKLKGDLSKSFDMKDLGPAQQILGMKIVRERTSRKLWLSQEKYIERVLE 1071

Query: 61   RVRLTDNRIVDTPLEPNARYS----PLDGIPLSDFSL--YRTIVGSLVYLTV-TCLDIAH 120
            R  + + + V TPL  + + S    P       + +   Y + VGSL+Y  V T  DIAH
Sbjct: 1072 RFNMKNAKPVSTPLAGHLKLSKKMCPTTVEEKGNMAKVPYSSAVGSLMYAMVCTRPDIAH 1131

Query: 121  VVHVVSQFVTAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDR 180
             V VVS+F+  P   HW  V  I RYLRGT    L F  +  + L+ Y+DAD  GD+ +R
Sbjct: 1132 AVGVVSRFLENPGKEHWEAVKWILRYLRGTTGDCLCFGGSDPI-LKGYTDADMAGDIDNR 1191

Query: 181  KSTTGFCIFFGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHH 240
            KS+TG+   F    ISW+SK    ++ S+T+AEY     T  E++WL+  L ++G++   
Sbjct: 1192 KSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLKRFLQELGLH-QK 1251

Query: 241  PTPLYCDNQSAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTK 300
               +YCD+QSAI ++ NS++H RTKHI++  H  R  +   S+ +  +S++    DM TK
Sbjct: 1252 EYVVYCDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKISTNENPADMLTK 1311

Query: 301  SHSASPFRFLSDKLSM 308
                + F    + + M
Sbjct: 1312 VVPRNKFELCKELVGM 1325

BLAST of MC02g_new0257 vs. ExPASy Swiss-Prot
Match: P92519 (Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 GN=AtMg00810 PE=4 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.9e-40
Identity = 86/217 (39.63%), Postives = 134/217 (61.75%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           +++TG  +  +  L  +LSS F+MKDLG +HYFL I++   P G  LSQ+KY   + +  
Sbjct: 10  ILLTGSSNTLLNMLIFQLSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYAEQILNNA 69

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
            + D + + TPL P    S +      D S +R+IVG+L YLT+T  DI++ V++V Q +
Sbjct: 70  GMLDCKPMSTPL-PLKLNSSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAVNIVCQRM 129

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
             P +  + ++ R+ RY++GT F  L     S L+++A+ D+DW G  + R+STTGFC F
Sbjct: 130 HEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTF 189

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVW 218
            G ++ISW +K+   +SRSST+ EYR +A T  E+ W
Sbjct: 190 LGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

BLAST of MC02g_new0257 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 162.9 bits (411), Expect = 5.7e-39
Identity = 101/312 (32.37%), Postives = 161/312 (51.60%), Query Frame = 0

Query: 1    MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
            ++I   D   + + K  L   F M DL  + +F+ I +        LSQS Y   +  + 
Sbjct: 1091 VVIATGDMTRMNNFKRYLMEKFRMTDLNEIKHFIGIRIEMQEDKIYLSQSAYVKKILSKF 1150

Query: 61   RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTV-TCLDIAHVVHVVSQF 120
             + +   V TPL     Y  L+     + +  R+++G L+Y+ + T  D+   V+++S++
Sbjct: 1151 NMENCNAVSTPLPSKINYELLNSDEDCN-TPCRSLIGCLMYIMLCTRPDLTTAVNILSRY 1210

Query: 121  VTAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLD--LRAYSDADWVGDLTDRKSTTGF 180
             +      W  + R+ RYL+GT    L+F    + +  +  Y D+DW G   DRKSTTG+
Sbjct: 1211 SSKNNSELWQNLKRVLRYLKGTIDMKLIFKKNLAFENKIIGYVDSDWAGSEIDRKSTTGY 1270

Query: 181  CI-FFGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLY 240
                F  +LI W +K+ + ++ SST+AEY  +     E +WL++LL  + I L +P  +Y
Sbjct: 1271 LFKMFDFNLICWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLENPIKIY 1330

Query: 241  CDNQSAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSAS 300
             DNQ  I IA N   HKR KHI+I  H  R  +Q   I L ++ +  Q+ D+FTK   A+
Sbjct: 1331 EDNQGCISIANNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTKPLPAA 1390

Query: 301  PFRFLSDKLSML 309
             F  L DKL +L
Sbjct: 1391 RFVELRDKLGLL 1401

BLAST of MC02g_new0257 vs. NCBI nr
Match: XP_023757860.1 (uncharacterized mitochondrial protein AtMg00810-like [Lactuca sativa])

HSP 1 Score: 474 bits (1220), Expect = 1.31e-165
Identity = 236/312 (75.64%), Postives = 267/312 (85.58%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           MIITGDD DGIESLK +L+  FAMKDLG+L YFL IEVA SPKGYLLSQ+KY  DLF+R 
Sbjct: 35  MIITGDDHDGIESLKRDLAHRFAMKDLGLLRYFLGIEVAQSPKGYLLSQTKYISDLFERA 94

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
           RLTDNR VDTP+E NA+YSP DG+PLSD SLYRTIVGSLVYLTV   DIAH VHVVSQFV
Sbjct: 95  RLTDNRTVDTPIESNAKYSPTDGVPLSDPSLYRTIVGSLVYLTVIRSDIAHAVHVVSQFV 154

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
           TAP  VHW  VLRI RYLRGTQF++L+F S+SSL+LRAYSDA+W  D+ DRKSTTG+C+F
Sbjct: 155 TAPTTVHWGAVLRILRYLRGTQFRTLVFPSSSSLELRAYSDANWDSDIYDRKSTTGYCVF 214

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
            GDSLISWKSKK DV+SRSST++EYR MA  TCEIVWLRWLLADMG+++  PTPLYCDN+
Sbjct: 215 LGDSLISWKSKKQDVVSRSSTESEYRAMAIATCEIVWLRWLLADMGVHISQPTPLYCDNE 274

Query: 241 SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
           SAI IA NSVFH+RTKHIEIDCH TRHHLQ+G+I+LP+V SSLQI D+ TK  +A  F F
Sbjct: 275 SAIMIAKNSVFHERTKHIEIDCHFTRHHLQKGTISLPYVPSSLQIADILTKPLTAPRFNF 334

Query: 301 LSDKLSMLIAVA 312
           LSDKLSMLIAVA
Sbjct: 335 LSDKLSMLIAVA 346

BLAST of MC02g_new0257 vs. NCBI nr
Match: XP_021980394.1 (uncharacterized mitochondrial protein AtMg00810-like [Helianthus annuus])

HSP 1 Score: 461 bits (1185), Expect = 8.90e-160
Identity = 232/308 (75.32%), Postives = 258/308 (83.77%), Query Frame = 0

Query: 5   GDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRVRLTD 64
           GDD DGIESLK +L+  FAMKDLG+L YFL IEVA    GYLLSQ+KY  DLF R RL+D
Sbjct: 72  GDDYDGIESLKHDLAHRFAMKDLGLLRYFLGIEVAQPHIGYLLSQTKYISDLFQRARLSD 131

Query: 65  NRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFVTAPA 124
           NR VDTPLE NARYSP DG+PL+D SLYRTIVGS VYLT+T  DIAH VHVVSQFVTAP 
Sbjct: 132 NRTVDTPLETNARYSPSDGVPLADPSLYRTIVGSFVYLTITRPDIAHAVHVVSQFVTAPT 191

Query: 125 IVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIFFGDS 184
            VHW  VL I RYLRGTQFQSLLF STSSL+LRAYSDADW  D  DRKSTTGFC+F GDS
Sbjct: 192 SVHWGAVLHILRYLRGTQFQSLLFPSTSSLELRAYSDADWDSDRNDRKSTTGFCVFLGDS 251

Query: 185 LISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQSAIQ 244
           LISW+SKK DV+SRSST+AEYR M  TTCEIVWLRWLLADMG+ +  PTPL+CDN+SA+Q
Sbjct: 252 LISWRSKKQDVVSRSSTEAEYRAMVVTTCEIVWLRWLLADMGVTISQPTPLHCDNKSAMQ 311

Query: 245 IACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRFLSDK 304
           IA NSVFH+RTKHIEIDCH TRHHLQ G+I+LP+V S+LQI D+FTK+ S S FRFL DK
Sbjct: 312 IAKNSVFHERTKHIEIDCHFTRHHLQLGTISLPYVPSALQIADIFTKALSVSRFRFLCDK 371

Query: 305 LSMLIAVA 312
           LSMLIA+A
Sbjct: 372 LSMLIAIA 379

BLAST of MC02g_new0257 vs. NCBI nr
Match: XP_042752762.1 (uncharacterized mitochondrial protein AtMg00810-like [Lactuca sativa])

HSP 1 Score: 458 bits (1178), Expect = 9.58e-160
Identity = 230/312 (73.72%), Postives = 262/312 (83.97%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           MIITGDD  GIESLK +L+  FAMKDLG+L YFL IEVA S KGYLLSQ+KY  DLF R 
Sbjct: 1   MIITGDDHGGIESLKHDLARRFAMKDLGLLRYFLGIEVAQSKKGYLLSQTKYISDLFTRA 60

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
            L+DN+ VDTPLE NARYSP +G+PLSD +LY T+VGSLVYLTVT  DIAH +H+V+QFV
Sbjct: 61  GLSDNQTVDTPLETNARYSPTNGVPLSDLNLYCTVVGSLVYLTVTRPDIAHAIHIVNQFV 120

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
           TAP  VHW  VLRI RYL GTQFQ+LLF STSSL+L AYSDADW GD  DRKSTTGFC+F
Sbjct: 121 TAPTSVHWEAVLRIMRYLCGTQFQTLLFSSTSSLELPAYSDADWDGDRHDRKSTTGFCVF 180

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
            G+SLISWKSKK DVISRSST+AEYR M  TTCEI+WLRWLLA+M +Y+  PTPL CDN+
Sbjct: 181 LGESLISWKSKKQDVISRSSTEAEYRAMTVTTCEIIWLRWLLANMEVYISSPTPLNCDNK 240

Query: 241 SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
           SAIQIA NS+FH+RTKHIEIDCH TRHHLQ G+I+LPFVSS+LQ+ ++FTK+ SAS FRF
Sbjct: 241 SAIQIAKNSIFHERTKHIEIDCHFTRHHLQLGTISLPFVSSTLQLANIFTKALSASRFRF 300

Query: 301 LSDKLSMLIAVA 312
           L DKLSMLIA A
Sbjct: 301 LCDKLSMLIAAA 312

BLAST of MC02g_new0257 vs. NCBI nr
Match: XP_015580508.1 (uncharacterized protein LOC107262008 [Ricinus communis])

HSP 1 Score: 456 bits (1172), Expect = 9.36e-158
Identity = 230/312 (73.72%), Postives = 263/312 (84.29%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           MIITGDD  GI  LKSEL+ CFAMKDLG L+Y L IEVA SPKGYLLSQSKY  D+F   
Sbjct: 72  MIITGDDVIGISLLKSELTRCFAMKDLGSLNY-LGIEVASSPKGYLLSQSKYISDIFGHA 131

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
           RL DN+ VD P+E NARYS  DG  L + SLYRT++GSL+YLT+T  DIA+VVH+VSQFV
Sbjct: 132 RLFDNKTVDNPIELNARYSTSDGSLLPNLSLYRTVIGSLIYLTITRPDIAYVVHIVSQFV 191

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
           T+P  +HW V+LRI RYLRGTQFQ+LLF STSSL+L AYSDADW GD TDRKSTTGF IF
Sbjct: 192 TSPTTIHWAVLLRILRYLRGTQFQTLLFSSTSSLELCAYSDADWAGDPTDRKSTTGFYIF 251

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
            GDSLISWKSKK DVISRSST+AEYR MASTTCEIVWLRWLLADMG++L  PTPL+CDN+
Sbjct: 252 LGDSLISWKSKKQDVISRSSTEAEYRAMASTTCEIVWLRWLLADMGVFLWQPTPLHCDNK 311

Query: 241 SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
           S IQIA NSVFH++TKHIEIDCH+TRHHLQ G++TLPFVSSSLQ+ ++F K  +AS FRF
Sbjct: 312 SVIQIAHNSVFHEQTKHIEIDCHITRHHLQNGTLTLPFVSSSLQLANLFIKYLTASCFRF 371

Query: 301 LSDKLSMLIAVA 312
           L DKLSML+A+A
Sbjct: 372 LFDKLSMLVAIA 382

BLAST of MC02g_new0257 vs. NCBI nr
Match: KAF3682436.1 (hypothetical protein FXO38_01293 [Capsicum annuum])

HSP 1 Score: 481 bits (1238), Expect = 3.95e-156
Identity = 244/312 (78.21%), Postives = 267/312 (85.58%), Query Frame = 0

Query: 1    MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
            MIITGDD  GIE LK +L+  FAMKDLG+L YFL IEVA S KGYLLSQ+KY  DLF R 
Sbjct: 1050 MIITGDDHSGIELLKYDLAHRFAMKDLGLLRYFLGIEVAQSKKGYLLSQTKYISDLFTRA 1109

Query: 61   RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
            RL+DNR VDTPLE NARYSP DG+PLSD SLYRTIVGSLVYLTVT  DIAH VHVVSQFV
Sbjct: 1110 RLSDNRTVDTPLETNARYSPSDGVPLSDPSLYRTIVGSLVYLTVTRPDIAHAVHVVSQFV 1169

Query: 121  TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
            TAP  VHW  VLRI RYLRGTQFQ+LLF STSSL+LRAYSDADW GD  DRKSTTGFC+F
Sbjct: 1170 TAPTSVHWGAVLRILRYLRGTQFQNLLFPSTSSLELRAYSDADWDGDRNDRKSTTGFCVF 1229

Query: 181  FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
             GDSLISWKSKK DV+SRSST+AEYR MA TTCEI+WLRWLLADMG+++  PTPL+CDN+
Sbjct: 1230 LGDSLISWKSKKQDVVSRSSTEAEYRAMAVTTCEIIWLRWLLADMGVHISMPTPLHCDNK 1289

Query: 241  SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
            SA+QIA NSVFH+RTKHIEIDCH T HHLQ G+I+LPFV SSLQI D+FTK+ SAS FRF
Sbjct: 1290 SAVQIAKNSVFHERTKHIEIDCHFTGHHLQLGTISLPFVPSSLQIADLFTKAQSASRFRF 1349

Query: 301  LSDKLSMLIAVA 312
            L DKLSMLIAVA
Sbjct: 1350 LCDKLSMLIAVA 1361

BLAST of MC02g_new0257 vs. ExPASy TrEMBL
Match: A0A2G2XMZ5 (RING-type E3 ubiquitin transferase OS=Capsicum baccatum OX=33114 GN=CQW23_01213 PE=4 SV=1)

HSP 1 Score: 463 bits (1191), Expect = 2.50e-156
Identity = 233/296 (78.72%), Postives = 256/296 (86.49%), Query Frame = 0

Query: 17  ELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRVRLTDNRIVDTPLEPNA 76
           +L+  FAMKDLG+L YFL IEVA S KGYLLSQ+KY  DLF R RL+DNR VDTPLE NA
Sbjct: 414 DLAHRFAMKDLGLLRYFLGIEVAQSKKGYLLSQTKYISDLFTRARLSDNRTVDTPLETNA 473

Query: 77  RYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFVTAPAIVHWVVVLRIPR 136
           RYSP DG+PLSD SLYRTIVGSLVYLTVT  DIAH VHVVSQFVTAP  VHW  VLRI R
Sbjct: 474 RYSPSDGVPLSDPSLYRTIVGSLVYLTVTRPDIAHAVHVVSQFVTAPTSVHWGAVLRILR 533

Query: 137 YLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIFFGDSLISWKSKKHDVI 196
           YLRGTQFQ+LLF STSSL+LRAYSDADW GD  DRKSTTGFC+F GDSLISWKSKK DV+
Sbjct: 534 YLRGTQFQNLLFPSTSSLELRAYSDADWDGDRNDRKSTTGFCVFLGDSLISWKSKKQDVV 593

Query: 197 SRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQSAIQIACNSVFHKRTK 256
           SRSST+AEYR MA TTCEI+WLRWLLADMG+++  PTPL+CDN+SA+QIA NSVFH+RTK
Sbjct: 594 SRSSTEAEYRAMAVTTCEIIWLRWLLADMGVHISMPTPLHCDNKSAVQIAKNSVFHERTK 653

Query: 257 HIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRFLSDKLSMLIAVA 312
           HIEIDCH TRHHLQ G+I+LPFV SSLQI D+FTK+ SAS FRFL DKLSMLIAVA
Sbjct: 654 HIEIDCHFTRHHLQLGTISLPFVPSSLQIADLFTKAQSASRFRFLCDKLSMLIAVA 709

BLAST of MC02g_new0257 vs. ExPASy TrEMBL
Match: A0A6J1E276 (uncharacterized protein LOC111025329 OS=Momordica charantia OX=3673 GN=LOC111025329 PE=4 SV=1)

HSP 1 Score: 441 bits (1134), Expect = 1.55e-152
Identity = 236/313 (75.40%), Postives = 244/313 (77.96%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           MIIT +DSDGI+SLKSELS CFAMKDLGML YFL IEV+YSPKGYLLSQSKY  DLFDR 
Sbjct: 89  MIITRNDSDGIKSLKSELSRCFAMKDLGMLLYFLGIEVSYSPKGYLLSQSKYIADLFDRA 148

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
           RLTDNRIVDTPLEPNARY  LD IPLSDFSLYRTIVGSLVYL VT  +I HVVHVVSQFV
Sbjct: 149 RLTDNRIVDTPLEPNARYFLLDEIPLSDFSLYRTIVGSLVYLIVTRPNITHVVHVVSQFV 208

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
           TAP  VHW  VLRI RYLRGTQFQSLLF STSSLDL AYSDADW  DLTDRKSTTGFC F
Sbjct: 209 TAPTTVHWAAVLRILRYLRGTQFQSLLFPSTSSLDLHAYSDADWADDLTDRKSTTGFCTF 268

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
            GDSLISWK                                  +MGIYLHHPTPLYCDNQ
Sbjct: 269 LGDSLISWK----------------------------------NMGIYLHHPTPLYCDNQ 328

Query: 241 SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
           SAIQIA NSVFH+RTKHI+IDCHVT HHLQR SITLP VSSS QI DMF K+HS S F F
Sbjct: 329 SAIQIARNSVFHERTKHIDIDCHVTHHHLQRRSITLPLVSSSPQIADMFIKTHSTSRFCF 367

Query: 301 LSDKLSMLIAVAS 313
           LSDKLSMLIAVAS
Sbjct: 389 LSDKLSMLIAVAS 367

BLAST of MC02g_new0257 vs. ExPASy TrEMBL
Match: A0A2G2V0H1 (NB-ARC domain-containing protein OS=Capsicum baccatum OX=33114 GN=CQW23_33919 PE=3 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 4.65e-152
Identity = 230/289 (79.58%), Postives = 251/289 (86.85%), Query Frame = 0

Query: 24  MKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRVRLTDNRIVDTPLEPNARYSPLDG 83
           MKDLG+L YFL IEVA S KGYLLSQ+KY  DLF R RL+DNR VDTPLE NARYSP DG
Sbjct: 1   MKDLGLLRYFLGIEVAQSKKGYLLSQTKYISDLFTRARLSDNRTVDTPLETNARYSPSDG 60

Query: 84  IPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFVTAPAIVHWVVVLRIPRYLRGTQF 143
           +PLSD SLYRTIVGSLVYLTVT  DIAH VHVVSQFVTAP  VHW  VLRI RYLRGTQF
Sbjct: 61  VPLSDPSLYRTIVGSLVYLTVTRPDIAHAVHVVSQFVTAPTSVHWGAVLRILRYLRGTQF 120

Query: 144 QSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIFFGDSLISWKSKKHDVISRSSTKA 203
           Q+LLF STSSL+LRAYSDADW GD  DRKSTTGFC+F GDSLISWKSKK DV+SRSST+A
Sbjct: 121 QNLLFPSTSSLELRAYSDADWDGDRNDRKSTTGFCVFLGDSLISWKSKKQDVVSRSSTEA 180

Query: 204 EYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQSAIQIACNSVFHKRTKHIEIDCH 263
           EYR MA TTCEI+WLRWLLADMG+++  PTPL+CDN+SA+QIA NSVFH+RTKHIEIDCH
Sbjct: 181 EYRAMAVTTCEIIWLRWLLADMGVHISMPTPLHCDNKSAVQIAKNSVFHERTKHIEIDCH 240

Query: 264 VTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRFLSDKLSMLIAVA 312
            TRHHLQ G+I+LPFV SSLQI D+FTK+ SAS FRFL DKLSMLIAVA
Sbjct: 241 FTRHHLQLGTISLPFVPSSLQIADLFTKAQSASRFRFLCDKLSMLIAVA 289

BLAST of MC02g_new0257 vs. ExPASy TrEMBL
Match: A0A6A4P152 (Putative RNA-directed DNA polymerase OS=Lupinus albus OX=3870 GN=Lalb_Chr17g0347791 PE=4 SV=1)

HSP 1 Score: 444 bits (1143), Expect = 1.97e-150
Identity = 227/313 (72.52%), Postives = 258/313 (82.43%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           MIITGDD DGI  LK++L+  F MKDLG L YFL IEVA SP+GYLLSQSKY  D+ D V
Sbjct: 297 MIITGDDIDGITKLKTQLAEQFEMKDLGTLTYFLGIEVASSPRGYLLSQSKYIADILDLV 356

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
            L+D + VD+PLE NARY+  DG+PLS+ +LYRT+VGSLVYLT+T  DIA+ VH+VSQFV
Sbjct: 357 SLSDTKDVDSPLETNARYTSSDGVPLSEPTLYRTLVGSLVYLTITRPDIAYAVHIVSQFV 416

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
           ++P+ VHW  VLRI RYLRGTQFQSLLF STSSL L AYSDAD  GD TDRKSTTGFCIF
Sbjct: 417 SSPSTVHWAAVLRILRYLRGTQFQSLLFSSTSSLTLSAYSDAD-CGDPTDRKSTTGFCIF 476

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
            GDSLISWKSKK DVISRSST+AEYR MA+TT EIVWLRWLLA +G+    PTP+YCDN+
Sbjct: 477 LGDSLISWKSKKQDVISRSSTEAEYRAMATTTAEIVWLRWLLASLGVDQPEPTPMYCDNK 536

Query: 241 SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRF 300
           SAIQIA NSVFH+RTKHIEIDCH TRHH Q G+ITLPF+SSSLQI D+FTK HS   FRF
Sbjct: 537 SAIQIAHNSVFHERTKHIEIDCHFTRHHFQHGTITLPFISSSLQIADLFTKPHSTQRFRF 596

Query: 301 LSDKLSMLIAVAS 313
           L++KLSML   AS
Sbjct: 597 LTNKLSMLSINAS 608

BLAST of MC02g_new0257 vs. ExPASy TrEMBL
Match: A0A2G2W2H9 (NB-ARC domain-containing protein OS=Capsicum baccatum OX=33114 GN=CQW23_23015 PE=3 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 1.92e-146
Identity = 230/289 (79.58%), Postives = 251/289 (86.85%), Query Frame = 0

Query: 24  MKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRVRLTDNRIVDTPLEPNARYSPLDG 83
           MKDLG+L YFL IEVA S KGYLLSQ+KY  DLF R RL+DNR VDTPLE NARYSP DG
Sbjct: 1   MKDLGLLRYFLGIEVAQSKKGYLLSQTKYISDLFTRARLSDNRTVDTPLETNARYSPSDG 60

Query: 84  IPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFVTAPAIVHWVVVLRIPRYLRGTQF 143
           +PLSD SLYRTIVGSLVYLTVT  DIAH VHVVSQFVTAP  VHW  VLRI RYLRGTQF
Sbjct: 61  VPLSDPSLYRTIVGSLVYLTVTRPDIAHAVHVVSQFVTAPTSVHWGAVLRILRYLRGTQF 120

Query: 144 QSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIFFGDSLISWKSKKHDVISRSSTKA 203
           Q+LLF STSSL+LRAYSDADW GD  DRKSTTGFC+F GDSLISWKSKK DV+SRSST+A
Sbjct: 121 QNLLFPSTSSLELRAYSDADWDGDRNDRKSTTGFCVFLGDSLISWKSKKQDVVSRSSTEA 180

Query: 204 EYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQSAIQIACNSVFHKRTKHIEIDCH 263
           EYR MA TTCEI+WLRWLLADMG+++  PTPL+CDN+SA+QIA NSVFH+RTKHIEIDCH
Sbjct: 181 EYRAMAVTTCEIIWLRWLLADMGVHISMPTPLHCDNKSAVQIAKNSVFHERTKHIEIDCH 240

Query: 264 VTRHHLQRGSITLPFVSSSLQIVDMFTKSHSASPFRFLSDKLSMLIAVA 312
            TRHHLQ G+I+LPFV SSLQI D+FTK+ SAS FRFL DKLSMLIAVA
Sbjct: 241 FTRHHLQLGTISLPFVPSSLQIADLFTKAQSASRFRFLCDKLSMLIAVA 289

BLAST of MC02g_new0257 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 229.6 bits (584), Expect = 3.6e-60
Identity = 119/287 (41.46%), Postives = 173/287 (60.28%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           +II  ++   ++ LKS+L SCF ++DLG L YFL +E+A S  G  + Q KY +DL D  
Sbjct: 287 IIICSNNDAAVDELKSQLKSCFKLRDLGPLKYFLGLEIARSAAGINICQRKYALDLLDET 346

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
            L   +    P++P+  +S   G    D   YR ++G L+YL +T LDI+  V+ +SQF 
Sbjct: 347 GLLGCKPSSVPMDPSVTFSAHSGGDFVDAKAYRRLIGRLMYLQITRLDISFAVNKLSQFS 406

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
            AP + H   V++I  Y++GT  Q L + S + + L+ +SDA +      R+ST G+C+F
Sbjct: 407 EAPRLAHQQAVMKILHYIKGTVGQGLFYSSQAEMQLQVFSDASFQSCKDTRRSTNGYCMF 466

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVWLRWLLADMGIYLHHPTPLYCDNQ 240
            G SLISWKSKK  V+S+SS +AEYR ++  T E++WL     ++ + L  PT L+CDN 
Sbjct: 467 LGTSLISWKSKKQQVVSKSSAEAEYRALSFATDEMMWLAQFFRELQLPLSKPTLLFCDNT 526

Query: 241 SAIQIACNSVFHKRTKHIEIDCHVTRHHLQRGSITLPFVSSSLQIVD 288
           +AI IA N+VFH+RTKHIE DCH  R      S+    +S S Q  D
Sbjct: 527 AAIHIATNAVFHERTKHIESDCHSVRER----SVYQATLSYSFQAYD 569

BLAST of MC02g_new0257 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 6.3e-41
Identity = 86/217 (39.63%), Postives = 134/217 (61.75%), Query Frame = 0

Query: 1   MIITGDDSDGIESLKSELSSCFAMKDLGMLHYFLDIEVAYSPKGYLLSQSKYRVDLFDRV 60
           +++TG  +  +  L  +LSS F+MKDLG +HYFL I++   P G  LSQ+KY   + +  
Sbjct: 10  ILLTGSSNTLLNMLIFQLSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYAEQILNNA 69

Query: 61  RLTDNRIVDTPLEPNARYSPLDGIPLSDFSLYRTIVGSLVYLTVTCLDIAHVVHVVSQFV 120
            + D + + TPL P    S +      D S +R+IVG+L YLT+T  DI++ V++V Q +
Sbjct: 70  GMLDCKPMSTPL-PLKLNSSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAVNIVCQRM 129

Query: 121 TAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAYSDADWVGDLTDRKSTTGFCIF 180
             P +  + ++ R+ RY++GT F  L     S L+++A+ D+DW G  + R+STTGFC F
Sbjct: 130 HEPTLADFDLLKRVLRYVKGTIFHGLYIHKNSKLNVQAFCDSDWAGCTSTRRSTTGFCTF 189

Query: 181 FGDSLISWKSKKHDVISRSSTKAEYRVMASTTCEIVW 218
            G ++ISW +K+   +SRSST+ EYR +A T  E+ W
Sbjct: 190 LGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

BLAST of MC02g_new0257 vs. TAIR 10
Match: ATMG00240.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 58.9 bits (141), Expect = 8.3e-09
Identity = 30/79 (37.97%), Postives = 47/79 (59.49%), Query Frame = 0

Query: 100 VYLTVTCLDIAHVVHVVSQFVTAPAIVHWVVVLRIPRYLRGTQFQSLLFLSTSSLDLRAY 159
           +YLT+T  D+   V+ +SQF +A        V ++  Y++GT  Q L + +TS L L+A+
Sbjct: 1   MYLTITRPDLTFAVNRLSQFSSASRTAQMQAVYKVLHYVKGTVGQGLFYSATSDLQLKAF 60

Query: 160 SDADWVGDLTDRKSTTGFC 179
           +D+DW      R+S TGFC
Sbjct: 61  ADSDWASCPDTRRSVTGFC 79

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94HW24.2e-5841.04Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT941.2e-5739.61Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
P109783.6e-4135.44Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P925198.9e-4039.63Uncharacterized mitochondrial protein AtMg00810 OS=Arabidopsis thaliana OX=3702 ... [more]
P041465.7e-3932.37Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Match NameE-valueIdentityDescription
XP_023757860.11.31e-16575.64uncharacterized mitochondrial protein AtMg00810-like [Lactuca sativa][more]
XP_021980394.18.90e-16075.32uncharacterized mitochondrial protein AtMg00810-like [Helianthus annuus][more]
XP_042752762.19.58e-16073.72uncharacterized mitochondrial protein AtMg00810-like [Lactuca sativa][more]
XP_015580508.19.36e-15873.72uncharacterized protein LOC107262008 [Ricinus communis][more]
KAF3682436.13.95e-15678.21hypothetical protein FXO38_01293 [Capsicum annuum][more]
Match NameE-valueIdentityDescription
A0A2G2XMZ52.50e-15678.72RING-type E3 ubiquitin transferase OS=Capsicum baccatum OX=33114 GN=CQW23_01213 ... [more]
A0A6J1E2761.55e-15275.40uncharacterized protein LOC111025329 OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A2G2V0H14.65e-15279.58NB-ARC domain-containing protein OS=Capsicum baccatum OX=33114 GN=CQW23_33919 PE... [more]
A0A6A4P1521.97e-15072.52Putative RNA-directed DNA polymerase OS=Lupinus albus OX=3870 GN=Lalb_Chr17g0347... [more]
A0A2G2W2H91.92e-14679.58NB-ARC domain-containing protein OS=Capsicum baccatum OX=33114 GN=CQW23_23015 PE... [more]
Match NameE-valueIdentityDescription
AT4G23160.13.6e-6041.46cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
ATMG00810.16.3e-4139.63DNA/RNA polymerases superfamily protein [more]
ATMG00240.18.3e-0937.97Gag-Pol-related retrotransposon family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 1..72
e-value: 4.6E-10
score: 39.5
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 16..222
NoneNo IPR availablePANTHERPTHR11439:SF268ATP-DEPENDENT DNA HELICASEcoord: 16..222
NoneNo IPR availableCDDcd09272RNase_HI_RT_Ty1coord: 157..292
e-value: 3.02721E-74
score: 222.729
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 21..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g_new0257.1MC02g_new0257.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
biological_process GO:0006955 immune response
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0043531 ADP binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity