MC02g_new0188 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g_new0188
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDisease resistance protein (TIR-NBS-LRR class) family
LocationMC02: 8516978 .. 8518002 (+)
RNA-Seq ExpressionMC02g_new0188
SyntenyMC02g_new0188
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTGCCTAACAACATTTTCGTTGAGAATCTGTCCGTAATAATTCTAGGGGGCTGTCTAAATTGTGAAAGGCTTCCAATGCTTGGGCAGTTAACAAACTTAAAGAGGCTTGATGTATATGACTTGCATAGTATTCGAAGTTTAGGAAATGAATTCTATGGAAATGATTGTAATCAAAGGACCTTATTCCCCAAGCTAAAGGCATTCTATGTCTCTGATATGGAGAACTTAGAGCAGTGGGAAGAAGTAACAGTTGCTTCAAATCTCACCACATTTCCTTGCCTTGAAAAGTTGAGCATTCGTACATGTCGCAGGTTAATGAACATTCCAGATGTTTTTGGTTCTCGTGACGAGAATGGGGTCGAGCACCTTCGTCTTCTGAATATTTCATATTGTGATGGAATGACCAAACTTCCAAACGGGCTACAATTTTGTCCTTCCATCGTACGTTTGGAAATAGAAAACTGTTCCAAATTGAGCTTAAGTCTGCAGAGGTTGTCTTATCTAAGAATTGAAGGCTTACACAAATTGCCAGAGGGGATAGCCCATCTCAAGAACTTGCAAGCACTGGCAATTTCTGGATGCATCCAAAATTATGACTTTTGCCCTCTCATGCATCTTCCTTCTCTTACAAAGCTTCATTTGTGTGACGAGCTGAAAAGTGCCACCCATCTTCCTCAACAACTTGAGCACTGCACTACTCTGCAATATCTAGTCATTGAAAAATTTGAGCATATGGAAGCTTTGCCAGAATGGTTGGGAAACCAGCTTACATCGTTGAAAGTATTATGTCTTGACTCTTGCAAGAATCTGAAACAGTTACCTTCCACAGAAGCAATGCTACGCCCCACCAAATTAAGTGCTTTCTATGTTGATGCATGTCCGCGGCTACAACTCGGTGAAGGTGACACGGAGCGGGCCAAACTTTCCCATCCAATTCCAGAAATTCGTGTTTACCTCCATCATATTTGGTTTTTTTGGTTCTTTTTAGGACCACCCTTGAGATAACTGTATGCAAATCTTCAC

mRNA sequence

ATGCTTGGGCAGTTAACAAACTTAAAGAGGCTTGATGTATATGACTTGCATAGTATTCGAAGTTTAGGAAATGAATTCTATGGAAATGATTGTAATCAAAGGACCTTATTCCCCAAGCTAAAGGCATTCTATGTCTCTGATATGGAGAACTTAGAGCAGTGGGAAGAAGTAACAGTTGCTTCAAATCTCACCACATTTCCTTGCCTTGAAAAGTTGAGCATTCGTACATGTCGCAGGTTAATGAACATTCCAGATGTTTTTGGTTCTCGTGACGAGAATGGGGTCGAGCACCTTCGTCTTCTGAATATTTCATATTGTGATGGAATGACCAAACTTCCAAACGGGCTACAATTTTGTCCTTCCATCGTACGTTTGGAAATAGAAAACTGTTCCAAATTGAGCTTAAGTCTGCAGAGGTTGTCTTATCTAAGAATTGAAGGCTTACACAAATTGCCAGAGGGGATAGCCCATCTCAAGAACTTGCAAGCACTGGCAATTTCTGGATGCATCCAAAATTATGACTTTTGCCCTCTCATGCATCTTCCTTCTCTTACAAAGCTTCATTTGTGTGACGAGCTGAAAAGTGCCACCCATCTTCCTCAACAACTTGAGCACTGCACTACTCTGCAATATCTAGTCATTGAAAAATTTGAGCATATGGAAGCTTTGCCAGAATGGTTGGGAAACCAGCTTACATCGTTGAAAGTATTATGTCTTGACTCTTGCAAGAATCTGAAACAGTTACCTTCCACAGAAGCAATGCTACGCCCCACCAAATTAAGTGCTTTCTATGTTGATGCATGTCCGCGGCTACAACTCGGTGAAGGTGACACGGAGCGGGCCAAACTTTCCCATCCAATTCCAGAAATTCGTGTTTACCTCCATCATATTTGGTTTTTTTGGTTCTTTTTAGGACCACCCTTGAGATAA

Coding sequence (CDS)

ATGCTTGGGCAGTTAACAAACTTAAAGAGGCTTGATGTATATGACTTGCATAGTATTCGAAGTTTAGGAAATGAATTCTATGGAAATGATTGTAATCAAAGGACCTTATTCCCCAAGCTAAAGGCATTCTATGTCTCTGATATGGAGAACTTAGAGCAGTGGGAAGAAGTAACAGTTGCTTCAAATCTCACCACATTTCCTTGCCTTGAAAAGTTGAGCATTCGTACATGTCGCAGGTTAATGAACATTCCAGATGTTTTTGGTTCTCGTGACGAGAATGGGGTCGAGCACCTTCGTCTTCTGAATATTTCATATTGTGATGGAATGACCAAACTTCCAAACGGGCTACAATTTTGTCCTTCCATCGTACGTTTGGAAATAGAAAACTGTTCCAAATTGAGCTTAAGTCTGCAGAGGTTGTCTTATCTAAGAATTGAAGGCTTACACAAATTGCCAGAGGGGATAGCCCATCTCAAGAACTTGCAAGCACTGGCAATTTCTGGATGCATCCAAAATTATGACTTTTGCCCTCTCATGCATCTTCCTTCTCTTACAAAGCTTCATTTGTGTGACGAGCTGAAAAGTGCCACCCATCTTCCTCAACAACTTGAGCACTGCACTACTCTGCAATATCTAGTCATTGAAAAATTTGAGCATATGGAAGCTTTGCCAGAATGGTTGGGAAACCAGCTTACATCGTTGAAAGTATTATGTCTTGACTCTTGCAAGAATCTGAAACAGTTACCTTCCACAGAAGCAATGCTACGCCCCACCAAATTAAGTGCTTTCTATGTTGATGCATGTCCGCGGCTACAACTCGGTGAAGGTGACACGGAGCGGGCCAAACTTTCCCATCCAATTCCAGAAATTCGTGTTTACCTCCATCATATTTGGTTTTTTTGGTTCTTTTTAGGACCACCCTTGAGATAA

Protein sequence

MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCPSIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMHLPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIRVYLHHIWFFWFFLGPPLR
Homology
BLAST of MC02g_new0188 vs. ExPASy Swiss-Prot
Match: F4J339 (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana OX=3702 GN=RPP1 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 6.3e-14
Identity = 85/340 (25.00%), Postives = 133/340 (39.12%), Query Frame = 0

Query: 4    QLTNLKRLDVYDLHSIRSLGN--------EFYGNDCNQRTLFP----KLKAFYVSDMENL 63
            QL NLK +D+     ++ L N        E    +C+     P    KL +  + D+EN 
Sbjct: 712  QLRNLKWMDLSYSSYLKELPNLSTATNLEELKLRNCSSLVELPSSIEKLTSLQILDLENC 771

Query: 64   EQWEEVTVASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTK 123
               E++    N T    L +L ++ C  L+ +P   G+       +L+ LNIS C  + K
Sbjct: 772  SSLEKLPAIENATK---LRELKLQNCSSLIELPLSIGT-----ATNLKQLNISGCSSLVK 831

Query: 124  LPNGLQFCPSIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQ 183
            LP+ +     +   ++ NCS L                 LP  I +L+NL  L + GC +
Sbjct: 832  LPSSIGDITDLEVFDLSNCSSLV---------------TLPSSIGNLQNLCKLIMRGCSK 891

Query: 184  NYDFCPLMHLPSLTKLHL--CDELKS----ATHL-------------PQQLEHCTTLQYL 243
                   ++L SL  L+L  C +LKS    +TH+             P  +   + L   
Sbjct: 892  LEALPININLKSLDTLNLTDCSQLKSFPEISTHISELRLKGTAIKEVPLSIMSWSPLADF 951

Query: 244  VIEKFEHM-------------------EALPEWLGNQLTSLKVLCLDSCKNLKQLPSTEA 294
             I  FE +                   + +P W+  +++ L+ L L++C NL  LP    
Sbjct: 952  QISYFESLMEFPHAFDIITKLHLSKDIQEVPPWV-KRMSRLRDLSLNNCNNLVSLPQLS- 1011

BLAST of MC02g_new0188 vs. ExPASy Swiss-Prot
Match: Q9SZ67 (Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.0e-08
Identity = 45/172 (26.16%), Postives = 93/172 (54.07%), Query Frame = 0

Query: 93   NGVEHLRLLNISYCDGMTKLPNGLQFCPSIVRLEIENC-SKLSLS-----LQRLSYLRIE 152
            + +E L+ + +SY D +TK+P  L    ++  +++E C S LSLS     L++L +L ++
Sbjct: 1255 SSLEKLKKMRLSYSDQLTKIPR-LSSATNLEHIDLEGCNSLLSLSQSISYLKKLVFLNLK 1314

Query: 153  GLHKLPE--GIAHLKNLQALAISGCIQNYDFCPLMHLPSLTKLHLCDELKSATHLPQQLE 212
            G  KL     +  L++L+ L +SGC +  +F  +   P++ +L++   +     +P  ++
Sbjct: 1315 GCSKLENIPSMVDLESLEVLNLSGCSKLGNFPEIS--PNVKELYMGGTM--IQEIPSSIK 1374

Query: 213  HCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLDSCKNLKQLPSTEAMLR 257
            +   L+ L +E   H++ LP  +  +L  L+ L L  C +L++ P +   ++
Sbjct: 1375 NLVLLEKLDLENSRHLKNLPTSI-YKLKHLETLNLSGCISLERFPDSSRRMK 1420

BLAST of MC02g_new0188 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 58/220 (26.36%), Postives = 102/220 (46.36%), Query Frame = 0

Query: 50  NLEQWEEVTVASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGM 109
           +L    +++  S  ++ P LE+L +  C  L++I    G+     ++ L  L++  C+ +
Sbjct: 662 DLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGN-----MKKLTTLSLRSCNKL 721

Query: 110 TKLPNGLQFCPSIVRLEIENCSKLS---------LSLQRLSYLRIEGLHKLPEGIAHLKN 169
             LP+ +    S+  L + NCSK            SL  L  L+   +  LP+ I  L++
Sbjct: 722 KNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELD-LKNTAIKDLPDSIGDLES 781

Query: 170 LQALAISGCIQNYDFCPL-MHLPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEH 229
           L++L +S C +   F     ++ SLT+L L  +  +   LP  +    +L+ L +     
Sbjct: 782 LESLYLSNCSKFEKFPEKGGNMKSLTELDL--KNTAIKDLPDSIGDLESLEILNLSDCAK 841

Query: 230 MEALPEWLGNQLTSLKVLCLDSCKNLKQLPSTEAMLRPTK 260
            E  PE  GN + SLK L L +   +K LP +   L+  K
Sbjct: 842 FEKFPEKGGN-MKSLKELDLQNTA-IKDLPDSIGDLKSLK 871

BLAST of MC02g_new0188 vs. ExPASy Swiss-Prot
Match: Q9FI14 (Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 70/259 (27.03%), Postives = 117/259 (45.17%), Query Frame = 0

Query: 2   LGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVAS 61
           +G LTNLK+L +    S+  L + F GN          LK   +S   +L     + + S
Sbjct: 724 IGNLTNLKKLFLNRCSSLVKLPSSF-GN-------VTSLKELNLSGCSSL-----LEIPS 783

Query: 62  NLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNIS---YCD----GMTKLPN 121
           ++     L+K+    C  L+ +P   G  +   ++ L LLN S    C      +T+L +
Sbjct: 784 SIGNIVNLKKVYADGCSSLVQLPSSIG--NNTNLKELHLLNCSSLMECPSSMLNLTRLED 843

Query: 122 -GLQFCPSIVRL-EIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQN 181
             L  C S+V+L  I N     ++LQ L       L +LP  I +  NL  L + GC   
Sbjct: 844 LNLSGCLSLVKLPSIGNV----INLQSLYLSDCSSLMELPFTIENATNLDTLYLDGCSNL 903

Query: 182 YDF-CPLMHLPSLTKLHL--CDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGN 241
            +    + ++ +L  L+L  C  LK    LP  +E+   LQ L + K   +  LP  +  
Sbjct: 904 LELPSSIWNITNLQSLYLNGCSSLK---ELPSLVENAINLQSLSLMKCSSLVELPSSIW- 959

Query: 242 QLTSLKVLCLDSCKNLKQL 249
           ++++L  L + +C +L +L
Sbjct: 964 RISNLSYLDVSNCSSLLEL 959

BLAST of MC02g_new0188 vs. ExPASy Swiss-Prot
Match: P0CB16 (Putative disease resistance protein At4g19050 OS=Arabidopsis thaliana OX=3702 GN=At4g19050 PE=3 SV=2)

HSP 1 Score: 56.2 bits (134), Expect = 7.5e-07
Identity = 79/346 (22.83%), Postives = 135/346 (39.02%), Query Frame = 0

Query: 5   LTNLKRLDVYDLHSIRSLGN---------EFYGNDCN------------QRTLFPKLKAF 64
           +   ++L+V D+H  R L +         ++ G + N              T   +L  F
Sbjct: 559 IVETRKLEVIDIHGARKLESYFDRVKDWKDYKGKNKNFAQLQLLEHLDFSETKIIRLPIF 618

Query: 65  YVSDMENLEQWEEVTVASNLTTFPCLEKLSIRTCRRLMNIP-----------DVFGSRD- 124
           ++ D  N           + +T P L +L +R C RL  +P           D  G+ D 
Sbjct: 619 HLKDSTN-----------DFSTMPILTRLLLRNCTRLKRLPQLRPLTNLQILDACGATDL 678

Query: 125 -------ENGVEHLRLLNISYCDGMTKLPNGLQFCPSIVRLE---IENCSKLS--LSLQR 184
                      + LR+L++S     T LP        +V L    + NCS +    S+++
Sbjct: 679 VEMLEVCLEEKKELRILDMS----KTSLPELADTIADVVNLNKLLLRNCSLIEELPSIEK 738

Query: 185 LSYLRI-----------------------------EGLHKLPEGIAHLKNLQALAISGCI 244
           L++L +                               L +LP+ I+ L NL+ L I  C 
Sbjct: 739 LTHLEVFDVSGCIKLKNINGSFGEMSYLHEVNLSETNLSELPDKISELSNLKELIIRKCS 798

Query: 245 QNYDFCPLMHLPSLTKLHL-----CDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPE 272
           +      L +L  LT L +     C EL++   +    E+ + L  + + +  ++  LP 
Sbjct: 799 K---LKTLPNLEKLTNLEIFDVSGCTELET---IEGSFENLSCLHKVNLSE-TNLGELPN 858

BLAST of MC02g_new0188 vs. NCBI nr
Match: XP_022148553.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 637 bits (1642), Expect = 7.17e-224
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA 60
           MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA
Sbjct: 435 MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA 494

Query: 61  SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP 120
           SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP
Sbjct: 495 SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP 554

Query: 121 SIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMH 180
           SIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMH
Sbjct: 555 SIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMH 614

Query: 181 LPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLD 240
           LPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLD
Sbjct: 615 LPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLD 674

Query: 241 SCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIRVYLHHIWFF 300
           SCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSH IPEIRVYLHHIWFF
Sbjct: 675 SCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHXIPEIRVYLHHIWFF 734

Query: 301 WFFLGPPLR 309
           WFFLGPPLR
Sbjct: 735 WFFLGPPLR 743

BLAST of MC02g_new0188 vs. NCBI nr
Match: XP_022148515.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 427 bits (1098), Expect = 5.66e-138
Identity = 216/295 (73.22%), Postives = 247/295 (83.73%), Query Frame = 0

Query: 1    MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA 60
            MLGQL NL+RL+VY LHS++S+ NEFYGNDCNQRTLFPKL+AF +SDMENLEQW+EVTVA
Sbjct: 786  MLGQLRNLERLEVYGLHSVQSISNEFYGNDCNQRTLFPKLRAFLLSDMENLEQWKEVTVA 845

Query: 61   SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP 120
            SNLTTFPCLE L+I  C +LMNIPDVFG  DENGVEHLR + IS CDG+ KLPNGLQFC 
Sbjct: 846  SNLTTFPCLESLNIWRCPKLMNIPDVFGYCDENGVEHLRHVTISGCDGLNKLPNGLQFCH 905

Query: 121  SIVRLEIENCSKLSLSLQ---RLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCP 180
            SI  L IE+CS L+LSLQ   RLS L I GLHKLPEG+AHLK L+A+ I+G IQ+YDF P
Sbjct: 906  SIEDLTIEDCSNLTLSLQNKNRLSSLSIGGLHKLPEGMAHLKKLEAMKINGGIQDYDFSP 965

Query: 181  LMHLPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVL 240
            LMHLPSLT LHLCD+L +ATH+P+Q+EHCTTL++L+IE FEH+EALPEW GN  TSLK L
Sbjct: 966  LMHLPSLTYLHLCDDLGTATHVPRQVEHCTTLKFLIIENFEHIEALPEWFGN-FTSLKRL 1025

Query: 241  CLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIRV 292
             L  C NLKQLPS EAMLR T+L A YVD CP+LQL EGDTERAKLSH +PEI V
Sbjct: 1026 GLYFCSNLKQLPSREAMLRLTELRALYVDRCPQLQLDEGDTERAKLSH-LPEIDV 1078

BLAST of MC02g_new0188 vs. NCBI nr
Match: XP_038900462.1 (disease resistance protein RGA2-like [Benincasa hispida])

HSP 1 Score: 315 bits (806), Expect = 2.90e-95
Identity = 174/307 (56.68%), Postives = 219/307 (71.34%), Query Frame = 0

Query: 1    MLGQLTNLKRLDVYDLHSIRSLGNEFYGN-DCNQRTLFPKLKAFYVSDMENLEQWEEVTV 60
            MLGQL NLK L++Y L S++S+G EFYGN + NQRT F KL+ F +  M NLE WEE+T 
Sbjct: 781  MLGQLNNLKELEIYGLDSVQSIGKEFYGNYESNQRTFFSKLERFILKGMINLEHWEEITT 840

Query: 61   -----ASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPN 120
                 +SN+TTFP L++L I  C +L NIP+VFGS DEN  EHL LL+IS+CD + KLPN
Sbjct: 841  HDDYASSNVTTFPHLKELKIHGCPKLSNIPNVFGSCDENEEEHLELLSISHCDQLMKLPN 900

Query: 121  GLQFCPSIVRLEIE-NCSKLSLSLQ---RLSYLRIEGLHKLPEGIAHLKNLQALAISGCI 180
            GLQFC SI  +EI+ N S LSL+ Q   +L +L I  L KLPE + HL  LQ++ I GCI
Sbjct: 901  GLQFCHSIKLMEIDHNGSYLSLNFQTKPKLVHLSIGPLEKLPEELGHLMKLQSVKIVGCI 960

Query: 181  QNYDFCPLMHLPSLTKLHLCDELKS--ATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLG 240
            QNYDF PLM LPSL +L L ++L+   AT LPQQL+H T L++L +++F  +E LPEWLG
Sbjct: 961  QNYDFSPLMQLPSLKELCLVEDLRGNKATQLPQQLQHLTALKFLSLQRFNAIETLPEWLG 1020

Query: 241  NQLTSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLG-EGDTERAKLSHPI 294
            N   SL  L + +C+NLK+LPS EAMLR TKLS  YV  CP++QLG EGD E AKLSH +
Sbjct: 1021 N-FVSLHTLSVSNCENLKRLPSREAMLRLTKLSHLYVIHCPKIQLGTEGDMEMAKLSH-L 1080

BLAST of MC02g_new0188 vs. NCBI nr
Match: AAT77098.1 (Fom-2 protein, partial [Cucumis melo])

HSP 1 Score: 290 bits (742), Expect = 1.66e-90
Identity = 160/302 (52.98%), Postives = 204/302 (67.55%), Query Frame = 0

Query: 1   MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTV- 60
           MLGQL NLK+L++Y+   ++ + NEFYGND NQR  FPKL+ F + DM NL+QWEEV   
Sbjct: 253 MLGQLNNLKKLEIYNFQELQIIDNEFYGNDLNQRRFFPKLEKFVMCDMVNLQQWEEVMTN 312

Query: 61  --ASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQ 120
             +SN+T FP L  L IR C +L+NIP+VF   DEN V+HL  L +S+C+ +TKLPNGL 
Sbjct: 313 DASSNITIFPNLRSLEIRRCPKLLNIPEVF---DENNVQHLESLIVSHCNKLTKLPNGLH 372

Query: 121 FCPSIVRLEIENCSKLSLSLQR---LSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYD 180
           FC SI   +   CS LS++++    L YL I  L KLPE + HL  L+ + I G +QNYD
Sbjct: 373 FCSSI---QCVKCSNLSINIRNKSELCYLNIGPLEKLPEDLCHLMKLRGMKIVGNMQNYD 432

Query: 181 FCPLMHLPSLTKLHLC-DELK--SATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQL 240
           F  L HLPSL ++ L  DEL   S T +PQQL+H T L++L IE F  +EALPEWLGN  
Sbjct: 433 FGILQHLPSLKQITLVEDELSNNSVTQIPQQLQHLTALEFLSIENFGGIEALPEWLGN-F 492

Query: 241 TSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIR 293
             L+ L L +CKNLK+LPSTEAML  TKL+      CP+L LG GD E AKLSH +P+I 
Sbjct: 493 VCLQTLSLFNCKNLKKLPSTEAMLCLTKLNRLDACRCPQLLLGNGDMEGAKLSH-LPQIS 546

BLAST of MC02g_new0188 vs. NCBI nr
Match: XP_004149742.2 (disease resistance protein RGA2 [Cucumis sativus] >KGN58559.1 hypothetical protein Csa_001108 [Cucumis sativus])

HSP 1 Score: 285 bits (728), Expect = 3.56e-84
Identity = 159/301 (52.82%), Postives = 206/301 (68.44%), Query Frame = 0

Query: 1    MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTV- 60
            MLGQL NLK L +     ++ + NEFYGND NQR  FPKL+ F +S M NLEQW+EV   
Sbjct: 786  MLGQLNNLKELQICSFEGLQVIDNEFYGNDPNQRRFFPKLEKFEISYMINLEQWKEVITN 845

Query: 61   --ASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQ 120
              +SN+T FP L+ L I  C +L+NIP  F   DEN ++HL  L +S C+ +TKLP+GLQ
Sbjct: 846  DESSNVTIFPNLKCLKIWGCPKLLNIPKAF---DENNMQHLESLILSCCNKLTKLPDGLQ 905

Query: 121  FCPSIVRLEIENCSKLSLSLQ---RLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYD 180
            FC SI  L I+ CS LS++++   +L YL I  L KLPE + HL NL+ + I G +QNYD
Sbjct: 906  FCSSIEGLTIDKCSNLSINMRNKPKLWYLIIGWLDKLPEDLCHLMNLRVMRIIGIMQNYD 965

Query: 181  FCPLMHLPSLTKLHLCDEL---KSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQL 240
            F  L HLPSL +L L ++L    S T +P+QL+H T LQ+L I+ F  +EALPEWLGN +
Sbjct: 966  FGILQHLPSLKQLVLEEDLLSNNSVTQIPEQLQHLTALQFLSIQHFRRIEALPEWLGNYV 1025

Query: 241  TSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIR 292
              L+ L L +CK LK+LPSTEAMLR TKL+  +V  CP+L L EGD ERAKLSH +PEI+
Sbjct: 1026 C-LQTLNLWNCKKLKKLPSTEAMLRLTKLNKLHVCDCPQLLLEEGDMERAKLSH-LPEIQ 1081

BLAST of MC02g_new0188 vs. ExPASy TrEMBL
Match: A0A6J1D4E7 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111017186 PE=4 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 3.47e-224
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA 60
           MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA
Sbjct: 435 MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA 494

Query: 61  SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP 120
           SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP
Sbjct: 495 SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP 554

Query: 121 SIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMH 180
           SIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMH
Sbjct: 555 SIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLMH 614

Query: 181 LPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLD 240
           LPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLD
Sbjct: 615 LPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVLCLD 674

Query: 241 SCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIRVYLHHIWFF 300
           SCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSH IPEIRVYLHHIWFF
Sbjct: 675 SCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHXIPEIRVYLHHIWFF 734

Query: 301 WFFLGPPLR 309
           WFFLGPPLR
Sbjct: 735 WFFLGPPLR 743

BLAST of MC02g_new0188 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 2.74e-138
Identity = 216/295 (73.22%), Postives = 247/295 (83.73%), Query Frame = 0

Query: 1    MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVA 60
            MLGQL NL+RL+VY LHS++S+ NEFYGNDCNQRTLFPKL+AF +SDMENLEQW+EVTVA
Sbjct: 786  MLGQLRNLERLEVYGLHSVQSISNEFYGNDCNQRTLFPKLRAFLLSDMENLEQWKEVTVA 845

Query: 61   SNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCP 120
            SNLTTFPCLE L+I  C +LMNIPDVFG  DENGVEHLR + IS CDG+ KLPNGLQFC 
Sbjct: 846  SNLTTFPCLESLNIWRCPKLMNIPDVFGYCDENGVEHLRHVTISGCDGLNKLPNGLQFCH 905

Query: 121  SIVRLEIENCSKLSLSLQ---RLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCP 180
            SI  L IE+CS L+LSLQ   RLS L I GLHKLPEG+AHLK L+A+ I+G IQ+YDF P
Sbjct: 906  SIEDLTIEDCSNLTLSLQNKNRLSSLSIGGLHKLPEGMAHLKKLEAMKINGGIQDYDFSP 965

Query: 181  LMHLPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTSLKVL 240
            LMHLPSLT LHLCD+L +ATH+P+Q+EHCTTL++L+IE FEH+EALPEW GN  TSLK L
Sbjct: 966  LMHLPSLTYLHLCDDLGTATHVPRQVEHCTTLKFLIIENFEHIEALPEWFGN-FTSLKRL 1025

Query: 241  CLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIRV 292
             L  C NLKQLPS EAMLR T+L A YVD CP+LQL EGDTERAKLSH +PEI V
Sbjct: 1026 GLYFCSNLKQLPSREAMLRLTELRALYVDRCPQLQLDEGDTERAKLSH-LPEIDV 1078

BLAST of MC02g_new0188 vs. ExPASy TrEMBL
Match: Q694W1 (Fom-2 protein (Fragment) OS=Cucumis melo OX=3656 GN=Fom-2 PE=4 SV=1)

HSP 1 Score: 290 bits (742), Expect = 8.05e-91
Identity = 160/302 (52.98%), Postives = 204/302 (67.55%), Query Frame = 0

Query: 1   MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTV- 60
           MLGQL NLK+L++Y+   ++ + NEFYGND NQR  FPKL+ F + DM NL+QWEEV   
Sbjct: 253 MLGQLNNLKKLEIYNFQELQIIDNEFYGNDLNQRRFFPKLEKFVMCDMVNLQQWEEVMTN 312

Query: 61  --ASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQ 120
             +SN+T FP L  L IR C +L+NIP+VF   DEN V+HL  L +S+C+ +TKLPNGL 
Sbjct: 313 DASSNITIFPNLRSLEIRRCPKLLNIPEVF---DENNVQHLESLIVSHCNKLTKLPNGLH 372

Query: 121 FCPSIVRLEIENCSKLSLSLQR---LSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYD 180
           FC SI   +   CS LS++++    L YL I  L KLPE + HL  L+ + I G +QNYD
Sbjct: 373 FCSSI---QCVKCSNLSINIRNKSELCYLNIGPLEKLPEDLCHLMKLRGMKIVGNMQNYD 432

Query: 181 FCPLMHLPSLTKLHLC-DELK--SATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQL 240
           F  L HLPSL ++ L  DEL   S T +PQQL+H T L++L IE F  +EALPEWLGN  
Sbjct: 433 FGILQHLPSLKQITLVEDELSNNSVTQIPQQLQHLTALEFLSIENFGGIEALPEWLGN-F 492

Query: 241 TSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIR 293
             L+ L L +CKNLK+LPSTEAML  TKL+      CP+L LG GD E AKLSH +P+I 
Sbjct: 493 VCLQTLSLFNCKNLKKLPSTEAMLCLTKLNRLDACRCPQLLLGNGDMEGAKLSH-LPQIS 546

BLAST of MC02g_new0188 vs. ExPASy TrEMBL
Match: A0A0A0LCB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G684170 PE=4 SV=1)

HSP 1 Score: 285 bits (728), Expect = 1.72e-84
Identity = 159/301 (52.82%), Postives = 206/301 (68.44%), Query Frame = 0

Query: 1    MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTV- 60
            MLGQL NLK L +     ++ + NEFYGND NQR  FPKL+ F +S M NLEQW+EV   
Sbjct: 786  MLGQLNNLKELQICSFEGLQVIDNEFYGNDPNQRRFFPKLEKFEISYMINLEQWKEVITN 845

Query: 61   --ASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQ 120
              +SN+T FP L+ L I  C +L+NIP  F   DEN ++HL  L +S C+ +TKLP+GLQ
Sbjct: 846  DESSNVTIFPNLKCLKIWGCPKLLNIPKAF---DENNMQHLESLILSCCNKLTKLPDGLQ 905

Query: 121  FCPSIVRLEIENCSKLSLSLQ---RLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYD 180
            FC SI  L I+ CS LS++++   +L YL I  L KLPE + HL NL+ + I G +QNYD
Sbjct: 906  FCSSIEGLTIDKCSNLSINMRNKPKLWYLIIGWLDKLPEDLCHLMNLRVMRIIGIMQNYD 965

Query: 181  FCPLMHLPSLTKLHLCDEL---KSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQL 240
            F  L HLPSL +L L ++L    S T +P+QL+H T LQ+L I+ F  +EALPEWLGN +
Sbjct: 966  FGILQHLPSLKQLVLEEDLLSNNSVTQIPEQLQHLTALQFLSIQHFRRIEALPEWLGNYV 1025

Query: 241  TSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSHPIPEIR 292
              L+ L L +CK LK+LPSTEAMLR TKL+  +V  CP+L L EGD ERAKLSH +PEI+
Sbjct: 1026 C-LQTLNLWNCKKLKKLPSTEAMLRLTKLNKLHVCDCPQLLLEEGDMERAKLSH-LPEIQ 1081

BLAST of MC02g_new0188 vs. ExPASy TrEMBL
Match: A0A4Y6JLT3 (Fom-2 protein (Fragment) OS=Cucumis melo var. cantalupensis OX=3658 GN=Fom-2 PE=4 SV=1)

HSP 1 Score: 254 bits (648), Expect = 1.30e-78
Identity = 147/294 (50.00%), Postives = 182/294 (61.90%), Query Frame = 0

Query: 1   MLGQLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTV- 60
           MLGQL NLK+L++     ++ + N+FYGND NQR  FPKL+ F + +M NLEQWEEV   
Sbjct: 117 MLGQLNNLKKLEICSFDGVQIIDNKFYGNDPNQRRFFPKLEKFVMQNMINLEQWEEVMTN 176

Query: 61  --ASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQ 120
             +SN+T FP                             +L+ L IS C  +TK+PNGLQ
Sbjct: 177 DASSNVTIFP-----------------------------NLKSLEISGCPKLTKIPNGLQ 236

Query: 121 FCPSIVRLEIENCSKLSLSLQR---LSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYD 180
           FC SI R++I  CS L ++++    L YL I  L KLPE + HL NL  + I G IQNYD
Sbjct: 237 FCSSIRRVKIYQCSNLGINMRNKPELWYLHIGPLDKLPEDLCHLMNLGVMTIVGNIQNYD 296

Query: 181 FCPLMHLPSLTKLHLC-DELK--SATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQL 240
           F  L HLPSL K+ L  DEL   S T +PQQL+H T+L++L IE F  +EALPEWLGN L
Sbjct: 297 FGILQHLPSLKKITLVEDELSNNSVTQIPQQLQHLTSLEFLSIENFGGIEALPEWLGN-L 356

Query: 241 TSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRLQLGEGDTERAKLSH 285
             L+ LC   C+NLK+LPSTEAMLR TKL+  Y   CP L L EGD ERAKLSH
Sbjct: 357 VCLQTLCFLCCRNLKKLPSTEAMLRLTKLNKLYACECPMLLLEEGDPERAKLSH 380

BLAST of MC02g_new0188 vs. TAIR 10
Match: AT3G44480.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 79.7 bits (195), Expect = 4.5e-15
Identity = 85/340 (25.00%), Postives = 133/340 (39.12%), Query Frame = 0

Query: 4    QLTNLKRLDVYDLHSIRSLGN--------EFYGNDCNQRTLFP----KLKAFYVSDMENL 63
            QL NLK +D+     ++ L N        E    +C+     P    KL +  + D+EN 
Sbjct: 712  QLRNLKWMDLSYSSYLKELPNLSTATNLEELKLRNCSSLVELPSSIEKLTSLQILDLENC 771

Query: 64   EQWEEVTVASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTK 123
               E++    N T    L +L ++ C  L+ +P   G+       +L+ LNIS C  + K
Sbjct: 772  SSLEKLPAIENATK---LRELKLQNCSSLIELPLSIGT-----ATNLKQLNISGCSSLVK 831

Query: 124  LPNGLQFCPSIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQ 183
            LP+ +     +   ++ NCS L                 LP  I +L+NL  L + GC +
Sbjct: 832  LPSSIGDITDLEVFDLSNCSSLV---------------TLPSSIGNLQNLCKLIMRGCSK 891

Query: 184  NYDFCPLMHLPSLTKLHL--CDELKS----ATHL-------------PQQLEHCTTLQYL 243
                   ++L SL  L+L  C +LKS    +TH+             P  +   + L   
Sbjct: 892  LEALPININLKSLDTLNLTDCSQLKSFPEISTHISELRLKGTAIKEVPLSIMSWSPLADF 951

Query: 244  VIEKFEHM-------------------EALPEWLGNQLTSLKVLCLDSCKNLKQLPSTEA 294
             I  FE +                   + +P W+  +++ L+ L L++C NL  LP    
Sbjct: 952  QISYFESLMEFPHAFDIITKLHLSKDIQEVPPWV-KRMSRLRDLSLNNCNNLVSLPQLS- 1011

BLAST of MC02g_new0188 vs. TAIR 10
Match: AT3G25510.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-14
Identity = 68/273 (24.91%), Postives = 119/273 (43.59%), Query Frame = 0

Query: 4    QLTNLKRLDVYDLHSIRSLGNEFYGNDCNQRTLFPKLKAFYVSDMENLEQWEEVTVASNL 63
            + TNLK+  +    S+  L   F GN  N + L          D+ N      V + S++
Sbjct: 780  KFTNLKKFILNGCSSLVEL--PFMGNATNLQNL----------DLGNCSSL--VELPSSI 839

Query: 64   TTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCPSIV 123
                 L+ L +  C  L+ +P   G+       +L +L++  C  + ++P  +    ++ 
Sbjct: 840  GNAINLQNLDLSNCSSLVKLPSFIGN-----ATNLEILDLRKCSSLVEIPTSIGHVTNLW 899

Query: 124  RLEIENCS---KLSLSLQRLSYLRIEGLH------KLPEGIAHLKNLQALAISGCIQNYD 183
            RL++  CS   +L  S+  +S L++  LH      KLP    H  NL  L +SGC    +
Sbjct: 900  RLDLSGCSSLVELPSSVGNISELQVLNLHNCSNLVKLPSSFGHATNLWRLDLSGCSSLVE 959

Query: 184  F-CPLMHLPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTS 243
                + ++ +L +L+LC+   +   LP  + +   L  L + + + +EALP  +   L S
Sbjct: 960  LPSSIGNITNLQELNLCN-CSNLVKLPSSIGNLHLLFTLSLARCQKLEALPSNI--NLKS 1019

Query: 244  LKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVD 267
            L+ L L  C   K  P        T +   Y+D
Sbjct: 1020 LERLDLTDCSQFKSFPEIS-----TNIECLYLD 1025

BLAST of MC02g_new0188 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 76.6 bits (187), Expect = 3.8e-14
Identity = 77/287 (26.83%), Postives = 120/287 (41.81%), Query Frame = 0

Query: 5   LTNLKRLDVYDL------------------HSIRSLGNEFYGNDCNQRTLFPKLKAFYVS 64
           L+NLK L+ YDL                    +R L  + Y         FP+       
Sbjct: 550 LSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCM 609

Query: 65  DMENLEQ-WEEVTVASNLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISY 124
              NLE+ W+ +    N      L+K+ +  C+ L+ +PD+      +   +L  LN+SY
Sbjct: 610 SNSNLEKLWDGIQPLRN------LKKMDLSRCKYLVEVPDL------SKATNLEELNLSY 669

Query: 125 CDGMTKLPNGLQFCPSIVRLEIENCSKLSLSLQRLSYLRIEGLHKLPEGIAHLKNLQALA 184
           C  +      ++  PSI  L+  +C  L+  +Q         L  +P GI  LK+L+ + 
Sbjct: 670 CQSL------VEVTPSIKNLKGLSCFYLTNCIQ---------LKDIPIGII-LKSLETVG 729

Query: 185 ISGCIQNYDFCPLMHLPSLT----KLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHME 244
           +SGC        L H P ++    +L+L         LP  +   + L  L +   + + 
Sbjct: 730 MSGC------SSLKHFPEISWNTRRLYLSS--TKIEELPSSISRLSCLVKLDMSDCQRLR 789

Query: 245 ALPEWLGNQLTSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDAC 269
            LP +LG+ L SLK L LD C+ L+ LP T   L  T L    V  C
Sbjct: 790 TLPSYLGH-LVSLKSLNLDGCRRLENLPDTLQNL--TSLETLEVSGC 797

BLAST of MC02g_new0188 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 73.2 bits (178), Expect = 4.2e-13
Identity = 59/220 (26.82%), Postives = 100/220 (45.45%), Query Frame = 0

Query: 62  NLTTFPCLEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCPS 121
           NL+T   L ++ +  C  L+ +P   G+       +++ L+I  C  + KLP+ +    +
Sbjct: 711 NLSTAINLLEMVLSDCSSLIELPSSIGN-----ATNIKSLDIQGCSSLLKLPSSIGNLIT 770

Query: 122 IVRLEIENCSKL---------SLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQN 181
           + RL++  CS L          ++L RL  +    L +LP  I +L NL+A    GC   
Sbjct: 771 LPRLDLMGCSSLVELPSSIGNLINLPRLDLMGCSSLVELPSSIGNLINLEAFYFHGCSSL 830

Query: 182 YDF-CPLMHLPSLTKLHLCDELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQL 241
            +    + +L SL  L+L   + S   +P  + +   L+ L +     +  LP  +GN L
Sbjct: 831 LELPSSIGNLISLKILYL-KRISSLVEIPSSIGNLINLKLLNLSGCSSLVELPSSIGN-L 890

Query: 242 TSLKVLCLDSCKNLKQLPSTEAMLRPTKLSAFYVDACPRL 272
            +LK L L  C +L +LP +   L    L   Y+  C  L
Sbjct: 891 INLKKLDLSGCSSLVELPLSIGNL--INLQELYLSECSSL 921

BLAST of MC02g_new0188 vs. TAIR 10
Match: AT3G04220.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 68.9 bits (167), Expect = 7.9e-12
Identity = 69/271 (25.46%), Postives = 113/271 (41.70%), Query Frame = 0

Query: 12  DVYDLHSIRSLGNEFY--GNDCNQRTLFPKLKAFYVSDMENLEQ-WEEVTVASNLTTFPC 71
           D++  H +   G   +    D + +  FP+   +    +  LE+ WE +    N      
Sbjct: 616 DLFSRHGVYYFGGRGHRVSLDYDSKLHFPRGLDYLPGKLSKLEKLWEGIQPLRN------ 675

Query: 72  LEKLSIRTCRRLMNIPDVFGSRDENGVEHLRLLNISYCDGMTKLPNGLQFCPSIVRLEIE 131
           LE L +   R L  +PD+      +   +L+ L+I  C  + KLP+ +    ++ ++ + 
Sbjct: 676 LEWLDLTCSRNLKELPDL------STATNLQRLSIERCSSLVKLPSSIGEATNLKKINLR 735

Query: 132 NCSKL---------SLSLQRLSYLRIEGLHKLPEGIAHLKNLQALAISGCIQNYDFCPLM 191
            C  L           +LQ L       L +LP    +L N+++L      + Y+   L+
Sbjct: 736 ECLSLVELPSSFGNLTNLQELDLRECSSLVELPTSFGNLANVESL------EFYECSSLV 795

Query: 192 HLPS----LTKLHLCD--ELKSATHLPQQLEHCTTLQYLVIEKFEHMEALPEWLGNQLTS 251
            LPS    LT L +    E  S   LP    + T LQ L + K   +  LP    N LT+
Sbjct: 796 KLPSTFGNLTNLRVLGLRECSSMVELPSSFGNLTNLQVLNLRKCSTLVELPSSFVN-LTN 855

Query: 252 LKVLCLDSCKNLKQLPSTEAMLRPTKLSAFY 265
           L+ L L  C +L  LPS+   +   K   FY
Sbjct: 856 LENLDLRDCSSL--LPSSFGNVTYLKRLKFY 865

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4J3396.3e-1425.00Probable disease resistance protein RPP1 OS=Arabidopsis thaliana OX=3702 GN=RPP1... [more]
Q9SZ671.0e-0826.16Probable WRKY transcription factor 19 OS=Arabidopsis thaliana OX=3702 GN=WRKY19 ... [more]
V9M2S51.2e-0726.36Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
Q9FI141.2e-0727.03Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=... [more]
P0CB167.5e-0722.83Putative disease resistance protein At4g19050 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
XP_022148553.17.17e-22499.68putative disease resistance protein RGA3 [Momordica charantia][more]
XP_022148515.15.66e-13873.22disease resistance protein RGA2-like [Momordica charantia][more]
XP_038900462.12.90e-9556.68disease resistance protein RGA2-like [Benincasa hispida][more]
AAT77098.11.66e-9052.98Fom-2 protein, partial [Cucumis melo][more]
XP_004149742.23.56e-8452.82disease resistance protein RGA2 [Cucumis sativus] >KGN58559.1 hypothetical prote... [more]
Match NameE-valueIdentityDescription
A0A6J1D4E73.47e-22499.68putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1D3402.74e-13873.22disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017... [more]
Q694W18.05e-9152.98Fom-2 protein (Fragment) OS=Cucumis melo OX=3656 GN=Fom-2 PE=4 SV=1[more]
A0A0A0LCB51.72e-8452.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G684170 PE=4 SV=1[more]
A0A4Y6JLT31.30e-7850.00Fom-2 protein (Fragment) OS=Cucumis melo var. cantalupensis OX=3658 GN=Fom-2 PE=... [more]
Match NameE-valueIdentityDescription
AT3G44480.14.5e-1525.00Disease resistance protein (TIR-NBS-LRR class) family [more]
AT3G25510.11.7e-1424.91disease resistance protein (TIR-NBS-LRR class), putative [more]
AT5G17680.13.8e-1426.83disease resistance protein (TIR-NBS-LRR class), putative [more]
AT1G69550.14.2e-1326.82disease resistance protein (TIR-NBS-LRR class) [more]
AT3G04220.17.9e-1225.46Disease resistance protein (TIR-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 134..275
e-value: 4.2E-20
score: 73.5
coord: 1..94
e-value: 1.3E-11
score: 45.6
NoneNo IPR availablePANTHERPTHR16083LEUCINE RICH REPEAT CONTAINING PROTEINcoord: 2..169
coord: 56..272
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 2..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g_new0188.1MC02g_new0188.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding