MC02g_new0122 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g_new0122
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein kinase domain-containing protein
LocationMC02: 5614355 .. 5617904 (+)
RNA-Seq ExpressionMC02g_new0122
SyntenyMC02g_new0122
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAAAATTGACTAACTACGACCCCAGCAAAGCAACACAATCAATCGCAGCGGGCCCCACGTTTCTTCCGCAGACATCATCACGTTCACAAACGAGACACGTCACCATCATGATGATCCCCGGCCCGCCGTCACATCAGATTGGTGGAGCACCAGTCCCCTCCAGGGACCTCACCCGCACTGGATAATTACCGTCACATATTTAATGGTCACCGCCCATACAGAAACAGAACAGAACAGAGCCACAGCCGGACTGTACAATCTAATGCAATTTCCTCTTCCCTTTTAGCTTTTTACCCATTTTTTCTTCTTCACGCTCCAAAAGAAGTTCATATCAAGCTTACGATCTTCGCTGCCGGATTTTGAAGATTCTTCTCATGTAATTCTTTGTTTTCCCGCGTTTGGATTTTGTGCGGCGGAGCCATGGCGACGGCATTGGAGTGCTGGTCGAGTAGGGCTAGTACGGACGAGGATTTGGTGGAGCAGGTGCTGATGAGAACTCAGGACAGATCGGAAGGCTCCAAGCCGGAGAGTTCGTTTGCCGTCGGAGCCAAGGAGTCGTCGGCGATGCAGAAACGGCTGCAGAGATTGAGTCGGAACGTGTCGGAGGCGGTGGCGTCGCTCAAAAACTCGTTGAATCTGGACTCTGCTCGCGATCCCTCGCCTACCAAGATCGAGGGCTCTAAGAAGGCTGTTTGGGGTAGTGTTGTGCGGAATCTTACTCAGCTCTATCCTGGTAGTCAGTTGCCTGAAAAGCTCGTCTCCAACATTCGCAAGCATTATGATTCCTTGCCTCTCAGGTTTGGACTCACATAACTCTGCTTCGCAATGTGGTATCTGAAGTTGAAAGTTGTGTAAATTGGAGAGTTGAAATGAAAACGAATTGAAATAATTATGAACTAATCGGAGCGGGAGCTACTCTAGTCTAGTAACTTAGTAGTGTTGTAATGAAAGATAGATCAGATATCTGTTTTGTGAATATTTGCTAATCATCTCCTGCTACCAAATTTCAACTAGACTGATGAGTATATTGCTGTCTCCCTGTTCCTTTTTCTGATTGTGGTTGTTTCATTCTTTATGTTGTTTTGTTGGTACGAACAGTTATGCTCAGGCGGGGTTCGAAATGAAAGATGTCTTTCTCCACATCAAATTGATAGAGCAGGCATCTGTTTATGATCACCCTGCAATCTTATTTCAAGAAGTAACTAATCATGACACTCAGAAACCTACAATGAAGTTCACATTTGCTTGCAACTCTTCGATTTCATGGTCGGCGATGTCTGGAGCGCTGGAGAGCGCAGGCATTCGCTGTGAGAAGATACAGATTTTTGAGAAGAAAAGATTTACCCTTGGAGTTATTCTTTTTGTAAATCAAGATGCCCAAGAGAAATTCTTTAAATCCAAAATTGAAAGTGCTCTAAAATTGGCTATTAAGAAGCCGAAAACCAATACAGTGAAACTCCCATTTGGATTTTGTGGATGCCAAGAAGGTAACGCCCGGGGGAAAGATTTGAGAGAAATAGAGGAGGATGCCGTTGAACAAAATTGCAGAAGTGGTTTTGACAACTCGAATTTGAATGAATGGCAAACGGTCCAATGTGGTGGACATGAGCTGGGTAAATGGTTGCTGAGCTCTGAGAATCTGGAGTTCACCGATCAGATTGGACCCAACTCGTTCAAGGGAGTCTACAAGGGCAAAAGAGTAGCCATAGAGAAGATTAAAGGGTGTGAAAAGGGAATTGCTTACAAGTTTGAGCTCCGTAAAGACTTGTTGGAGCTGATGACATGTGGGCACAAGAACATTTTGATGTTCTATGGTGTTTGTATTGATGAAAATCATGGCTTGTGTGTTGTAACCAAACTAATGGAAGGCGGATCGGTTCATGAATTGATGCTGAAAAACAAAAGGTTTCAGACAAAAGAAATAACCAGGATTGCTATTGATGTTGCAGAAGGAATCAAATTCATGAATGACCATGGTGTTGCCTATCGGGATCTTAACGCACAAAGAATTTTGTTAGATAAGAATGGCAGTGCTTGTTTGGGTGACATGGGCATACTCACTGCATGCAAAAACTTGGGCGAGGCAATGGAGTACGAAACCGATGGTTATCGGTGGCTAGCTCCCGAGGTCTGTTTGCTTTAATTCCGACTACACTTACTATAATCTAAAGCAAGTTTCAGTTATACTACACAGAAAAGAAATTAGTATATATCTCGCTGCTTTTAGCTAAATAACACGTTATCTTCTCGCTTTTTTGTGATGTTATTAGTGTTTCGAGTCATTGTACTAAACTGCTTAGGAGTAGGACGACACCTTTTTCAATTAAAGAAACTTTATGTCACTTCCTCCATGATGGTCGAGTGTGTAGCATACAGTCATGATTGAAGGTGCTTTAGACATCAGCGTTGTGTTTATATTTTTCTAGGCACCCTTATATTCTTCAGACCATTAAGATTAGATTTCACTGCAGCACCCATCTATTTGGCCGCAACTTTGAATTGTTGCCACTTTCTGCTTAATTGGTTCCATTTGTGGAGCCCGCCATGTTAAAGCTCCTTTAGAGGGAACCTGCTCCTTTCTAGTTTCTTCTAGGGGATTTGAAATTCCTGTAGAGCATCTCATCTCACTGCTATCCTTTACTTGTTGGCCTCTTTCTGTATATCTCCATTTGATTGGTAAATAATGAATGAGCGAAGGGTGTTAATTTGTTACCGTTGGCTTGGCTATCTGGTTTGGCACAGATTATCGCTGGCGACCCGGAGAGTGTCAACGAGACATGGATGAGTAATGCATACAGTTTTGGAATGGTAATATGGGAGATGGTGAGCGGTGAGGCGGCTTATGGCGCATATTCGCCAGTGCAGGCGGCAGTCGGTATAGCTGCTTGTGGTCTTAGACCTGATCTTCCAAAGGACTGCCCATCCACCCTGAAATCCCTGATGATCAAATGCTGGAACACTTGCCCATCAAAGCGCCCCCAGTTCTCTGAAATTTTATCAGTGCTGCTGGACTCCAACAATAACAACCATAGATAAATTAAAGTGAGTATTTCCTTTTAATCTTTATGGATCCAAAGTCTACTCTTGTGAAGTTACTGTAAAGGGTTCCTAATTTTCATGTAGAAGATCATATGATGCGTTCAATGTAGATGGTGAGAAGTTTGTGTTTTCAGATTTGAATCTAATCTCATGTTACCATGGATAAGAAAAAAGATAGAAAAACAAAACGCTAATGATCTCCTCTCGAGTTTGAACCGTAGGAAAATCAACTCTCTTACCAAGTTACCACCATGAGAAATCGAAAGATCTTTCTTTCCCTTTCTTTTTATGGGTGAATGGTTAAAGCGTCCAACCTATACCGATCTAAAAAAGGACAAAATTCGTAGGTTCGATTCTTGCTGGATGCATTTGGAATGTTCAGTTCAATCGCTGTTCACGGTTGTTACTATTATTATTATTATTTTCCTTTTTTGGTCTACAATGACTTTGCTACGTCCTTCTGTAAGACCATTGTTTAGTTTGTGAATGACAAGGAAGTTCACATCTGT

mRNA sequence

ATGGCGACGGCATTGGAGTGCTGGTCGAGTAGGGCTAGTACGGACGAGGATTTGGTGGAGCAGGTGCTGATGAGAACTCAGGACAGATCGGAAGGCTCCAAGCCGGAGAGTTCGTTTGCCGTCGGAGCCAAGGAGTCGTCGGCGATGCAGAAACGGCTGCAGAGATTGAGTCGGAACGTGTCGGAGGCGGTGGCGTCGCTCAAAAACTCGTTGAATCTGGACTCTGCTCGCGATCCCTCGCCTACCAAGATCGAGGGCTCTAAGAAGGCTGTTTGGGGTAGTGTTGTGCGGAATCTTACTCAGCTCTATCCTGGTAGTCAGTTGCCTGAAAAGCTCGTCTCCAACATTCGCAAGCATTATGATTCCTTGCCTCTCAGTTATGCTCAGGCGGGGTTCGAAATGAAAGATGTCTTTCTCCACATCAAATTGATAGAGCAGGCATCTGTTTATGATCACCCTGCAATCTTATTTCAAGAAGTAACTAATCATGACACTCAGAAACCTACAATGAAGTTCACATTTGCTTGCAACTCTTCGATTTCATGGTCGGCGATGTCTGGAGCGCTGGAGAGCGCAGGCATTCGCTGTGAGAAGATACAGATTTTTGAGAAGAAAAGATTTACCCTTGGAGTTATTCTTTTTGTAAATCAAGATGCCCAAGAGAAATTCTTTAAATCCAAAATTGAAAGTGCTCTAAAATTGGCTATTAAGAAGCCGAAAACCAATACAGTGAAACTCCCATTTGGATTTTGTGGATGCCAAGAAGGTAACGCCCGGGGGAAAGATTTGAGAGAAATAGAGGAGGATGCCGTTGAACAAAATTGCAGAAGTGGTTTTGACAACTCGAATTTGAATGAATGGCAAACGGTCCAATGTGGTGGACATGAGCTGGGTAAATGGTTGCTGAGCTCTGAGAATCTGGAGTTCACCGATCAGATTGGACCCAACTCGTTCAAGGGAGTCTACAAGGGCAAAAGAGTAGCCATAGAGAAGATTAAAGGGTGTGAAAAGGGAATTGCTTACAAGTTTGAGCTCCGTAAAGACTTGTTGGAGCTGATGACATGTGGGCACAAGAACATTTTGATGTTCTATGGTGTTTGTATTGATGAAAATCATGGCTTGTGTGTTGTAACCAAACTAATGGAAGGCGGATCGGTTCATGAATTGATGCTGAAAAACAAAAGGTTTCAGACAAAAGAAATAACCAGGATTGCTATTGATGTTGCAGAAGGAATCAAATTCATGAATGACCATGGTGTTGCCTATCGGGATCTTAACGCACAAAGAATTTTGTTAGATAAGAATGGCAGTGCTTGTTTGGGTGACATGGGCATACTCACTGCATGCAAAAACTTGGGCGAGGCAATGGAGTACGAAACCGATGGTTATCGGTGGCTAGCTCCCGAGATTATCGCTGGCGACCCGGAGAGTGTCAACGAGACATGGATGAGTAATGCATACAGTTTTGGAATGGTAATATGGGAGATGGTGAGCGGTGAGGCGGCTTATGGCGCATATTCGCCAGTGCAGGCGGCAGTCGGTATAGCTGCTTGTGGTCTTAGACCTGATCTTCCAAAGGACTGCCCATCCACCCTGAAATCCCTGATGATCAAATGCTGGAACACTTGCCCATCAAAGCGCCCCCAGTTCTCTGAAATTTTATCAGTGCTGCTGGACTCCAACAATAACAACCATAGATAA

Coding sequence (CDS)

ATGGCGACGGCATTGGAGTGCTGGTCGAGTAGGGCTAGTACGGACGAGGATTTGGTGGAGCAGGTGCTGATGAGAACTCAGGACAGATCGGAAGGCTCCAAGCCGGAGAGTTCGTTTGCCGTCGGAGCCAAGGAGTCGTCGGCGATGCAGAAACGGCTGCAGAGATTGAGTCGGAACGTGTCGGAGGCGGTGGCGTCGCTCAAAAACTCGTTGAATCTGGACTCTGCTCGCGATCCCTCGCCTACCAAGATCGAGGGCTCTAAGAAGGCTGTTTGGGGTAGTGTTGTGCGGAATCTTACTCAGCTCTATCCTGGTAGTCAGTTGCCTGAAAAGCTCGTCTCCAACATTCGCAAGCATTATGATTCCTTGCCTCTCAGTTATGCTCAGGCGGGGTTCGAAATGAAAGATGTCTTTCTCCACATCAAATTGATAGAGCAGGCATCTGTTTATGATCACCCTGCAATCTTATTTCAAGAAGTAACTAATCATGACACTCAGAAACCTACAATGAAGTTCACATTTGCTTGCAACTCTTCGATTTCATGGTCGGCGATGTCTGGAGCGCTGGAGAGCGCAGGCATTCGCTGTGAGAAGATACAGATTTTTGAGAAGAAAAGATTTACCCTTGGAGTTATTCTTTTTGTAAATCAAGATGCCCAAGAGAAATTCTTTAAATCCAAAATTGAAAGTGCTCTAAAATTGGCTATTAAGAAGCCGAAAACCAATACAGTGAAACTCCCATTTGGATTTTGTGGATGCCAAGAAGGTAACGCCCGGGGGAAAGATTTGAGAGAAATAGAGGAGGATGCCGTTGAACAAAATTGCAGAAGTGGTTTTGACAACTCGAATTTGAATGAATGGCAAACGGTCCAATGTGGTGGACATGAGCTGGGTAAATGGTTGCTGAGCTCTGAGAATCTGGAGTTCACCGATCAGATTGGACCCAACTCGTTCAAGGGAGTCTACAAGGGCAAAAGAGTAGCCATAGAGAAGATTAAAGGGTGTGAAAAGGGAATTGCTTACAAGTTTGAGCTCCGTAAAGACTTGTTGGAGCTGATGACATGTGGGCACAAGAACATTTTGATGTTCTATGGTGTTTGTATTGATGAAAATCATGGCTTGTGTGTTGTAACCAAACTAATGGAAGGCGGATCGGTTCATGAATTGATGCTGAAAAACAAAAGGTTTCAGACAAAAGAAATAACCAGGATTGCTATTGATGTTGCAGAAGGAATCAAATTCATGAATGACCATGGTGTTGCCTATCGGGATCTTAACGCACAAAGAATTTTGTTAGATAAGAATGGCAGTGCTTGTTTGGGTGACATGGGCATACTCACTGCATGCAAAAACTTGGGCGAGGCAATGGAGTACGAAACCGATGGTTATCGGTGGCTAGCTCCCGAGATTATCGCTGGCGACCCGGAGAGTGTCAACGAGACATGGATGAGTAATGCATACAGTTTTGGAATGGTAATATGGGAGATGGTGAGCGGTGAGGCGGCTTATGGCGCATATTCGCCAGTGCAGGCGGCAGTCGGTATAGCTGCTTGTGGTCTTAGACCTGATCTTCCAAAGGACTGCCCATCCACCCTGAAATCCCTGATGATCAAATGCTGGAACACTTGCCCATCAAAGCGCCCCCAGTTCTCTGAAATTTTATCAGTGCTGCTGGACTCCAACAATAACAACCATAGATAA

Protein sequence

MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNVSEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHYDSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSISWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPKTNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR
Homology
BLAST of MC02g_new0122 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 6.1e-39
Identity = 86/263 (32.70%), Postives = 149/263 (56.65%), Query Frame = 0

Query: 300 WLLSSENLEFTDQIGPNSFKGVYKG----KRVAIEKIKGCEKGIAYKFELRKDLLELMTC 359
           W ++ ++L+F  +I   S+  +YKG    + VAI+ +K        + E  +++  +   
Sbjct: 283 WEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFIMRKV 342

Query: 360 GHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR-FQTKEITRIAIDVAEGIKF 419
            HKN++ F G C    H LC+VT+ M GGSV++ + K K  F+   + ++AID+ +G+ +
Sbjct: 343 RHKNVVQFIGACTKPPH-LCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKGMSY 402

Query: 420 MNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGD 479
           ++ + + +RDL A  +L+D+N    + D G+       G  M  ET  YRW+APE+I   
Sbjct: 403 LHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTG-VMTAETGTYRWMAPEVIEHK 462

Query: 480 PESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKS 539
           P        ++ +S+G+V+WE+++G+  Y   +P+QAAVG+   GLRP +PK+    L  
Sbjct: 463 PYD----HKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAE 522

Query: 540 LMIKCWNTCPSKRPQFSEILSVL 558
           L+ + W    ++RP FSEI+  L
Sbjct: 523 LLERLWEHDSTQRPDFSEIIEQL 539

BLAST of MC02g_new0122 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 6.7e-38
Identity = 81/264 (30.68%), Postives = 147/264 (55.68%), Query Frame = 0

Query: 299 KWLLSSENLEFTDQIGPNS----FKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMT 358
           +W +  + L+   ++   S    F+G Y  + VAI+ +K          E  +++  +  
Sbjct: 284 EWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRK 343

Query: 359 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR-FQTKEITRIAIDVAEGIK 418
             HKN++ F G C   +  LC+VT+ M  GS+++ + K+K  F+ + + ++A+DV++G+ 
Sbjct: 344 VRHKNVVQFIGAC-TRSPNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMN 403

Query: 419 FMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAG 478
           +++ + + +RDL    +L+D++    + D G+       G  M  ET  YRW+APE+I  
Sbjct: 404 YLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESG-VMTAETGTYRWMAPEVIEH 463

Query: 479 DPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLK 538
            P        ++ +S+ +V+WE+++GE  Y   +P+QAAVG+   GLRP +PK+    L 
Sbjct: 464 KPYD----HRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLT 523

Query: 539 SLMIKCWNTCPSKRPQFSEILSVL 558
            L+ KCW   P+ RP F+EI+ +L
Sbjct: 524 ELLEKCWQQDPALRPNFAEIIEML 541

BLAST of MC02g_new0122 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 159.1 bits (401), Expect = 1.5e-37
Identity = 80/264 (30.30%), Postives = 147/264 (55.68%), Query Frame = 0

Query: 299 KWLLSSENLEFTDQIGPNSF----KGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMT 358
           +W +    L+   ++   S+    +G Y  + VAI+ +K          E  +++  +  
Sbjct: 278 EWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRK 337

Query: 359 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK-RFQTKEITRIAIDVAEGIK 418
             HKN++ F G C   +  LC+VT+ M  GS+++ + K K  F+ + + ++A+DVA+G+ 
Sbjct: 338 VRHKNVVQFLGAC-TRSPTLCIVTEFMARGSIYDFLHKQKCAFKLQTLLKVALDVAKGMS 397

Query: 419 FMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAG 478
           +++ + + +RDL    +L+D++G   + D G+       G  M  ET  YRW+APE+I  
Sbjct: 398 YLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESG-VMTAETGTYRWMAPEVIEH 457

Query: 479 DPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLK 538
            P +      ++ +S+ +V+WE+++G+  Y   +P+QAAVG+   GLRP +PK     +K
Sbjct: 458 KPYN----HKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVK 517

Query: 539 SLMIKCWNTCPSKRPQFSEILSVL 558
            L+ +CW+  P +RP F EI+ +L
Sbjct: 518 GLLERCWHQDPEQRPLFEEIIEML 535

BLAST of MC02g_new0122 vs. ExPASy Swiss-Prot
Match: Q7TQP6 (Serine/threonine-protein kinase TNNI3K OS=Rattus norvegicus OX=10116 GN=Tnni3k PE=2 SV=3)

HSP 1 Score: 142.9 bits (359), Expect = 1.1e-32
Identity = 87/269 (32.34%), Postives = 144/269 (53.53%), Query Frame = 0

Query: 302 LSSENLEFTDQIGPNSFKGVYKG----KRVAIEKIKG---CEKGIAYKFELRKDLLELMT 361
           L    +EF + IG  SF  VYKG    K VAI++ +    C K     F   +++  L  
Sbjct: 458 LQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMF--CREVSILCQ 517

Query: 362 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR---FQTKEITRIAIDVAEG 421
             H  ++ F G C+D+     +VT+ + GGS+  L+ + KR    Q+K I  IA+DVA+G
Sbjct: 518 LNHPCVVQFVGACLDDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLI--IAVDVAKG 577

Query: 422 IKFMND--HGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGE-AMEYETDGYRWLAP 481
           +++++     + +RDLN+  ILL ++G A + D G     ++L E  M  +    RW+AP
Sbjct: 578 MEYLHSLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAP 637

Query: 482 EIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDC 541
           E+     +    T  ++ +S+ + +WE+++GE  +    P  AA  +A   +RP +    
Sbjct: 638 EVFT---QCTRYTIKADVFSYSLCLWELLTGEIPFAHLKPAAAAADMAYHHIRPPIGYSI 697

Query: 542 PSTLKSLMIKCWNTCPSKRPQFSEILSVL 558
           P  + SL+I+ WN CP  RP+FSE++S L
Sbjct: 698 PKPISSLLIRGWNACPEGRPEFSEVVSKL 719

BLAST of MC02g_new0122 vs. ExPASy Swiss-Prot
Match: Q5GIG6 (Serine/threonine-protein kinase TNNI3K OS=Mus musculus OX=10090 GN=Tnni3k PE=2 SV=4)

HSP 1 Score: 139.8 bits (351), Expect = 9.4e-32
Identity = 85/269 (31.60%), Postives = 143/269 (53.16%), Query Frame = 0

Query: 302 LSSENLEFTDQIGPNSFKGVYKG----KRVAIEKIKG---CEKGIAYKFELRKDLLELMT 361
           L    +EF + IG  SF  VYKG    K VAI++ +    C K     F   +++  L  
Sbjct: 457 LQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMF--CREVSILCQ 516

Query: 362 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR---FQTKEITRIAIDVAEG 421
             H  ++ F G C+D+     +VT+ + GGS+  L+ + KR    Q+K I  IA+DVA+G
Sbjct: 517 LNHPCVVQFVGACLDDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLI--IAVDVAKG 576

Query: 422 IKFMND--HGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGE-AMEYETDGYRWLAP 481
           +++++     + +RDLN+  ILL ++G A + D G     ++L E  M  +    RW+AP
Sbjct: 577 MEYLHSLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAP 636

Query: 482 EIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDC 541
           E+     +    T  ++ +S+ + +WE+++GE  +    P  AA  +A   +RP +    
Sbjct: 637 EVFT---QCTRYTIKADVFSYALCLWELLTGEIPFAHLKPAAAAADMAYHHIRPPIGYSI 696

Query: 542 PSTLKSLMIKCWNTCPSKRPQFSEILSVL 558
           P  + SL+++ WN CP  RP+FSE++  L
Sbjct: 697 PKPISSLLMRGWNACPEGRPEFSEVVRKL 718

BLAST of MC02g_new0122 vs. NCBI nr
Match: XP_022148439.1 (dual specificity protein kinase shkB isoform X2 [Momordica charantia])

HSP 1 Score: 1136 bits (2939), Expect = 0.0
Identity = 564/566 (99.65%), Postives = 565/566 (99.82%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKKRFTLGV+LFVNQDAQEKFFKSKIESALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVVLFVNQDAQEKFFKSKIESALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKW 300
           TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKW
Sbjct: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKW 300

Query: 301 LLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNI 360
           LLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNI
Sbjct: 301 LLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNI 360

Query: 361 LMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRIAIDVAEGIKFMNDHGV 420
           LMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRI IDVAEGIKFMNDHGV
Sbjct: 361 LMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRIGIDVAEGIKFMNDHGV 420

Query: 421 AYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNE 480
           AYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNE
Sbjct: 421 AYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNE 480

Query: 481 TWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCW 540
           TWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCW
Sbjct: 481 TWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCW 540

Query: 541 NTCPSKRPQFSEILSVLLDSNNNNHR 566
           NTCPSKRPQFSEILSVLLDSNNNNHR
Sbjct: 541 NTCPSKRPQFSEILSVLLDSNNNNHR 566

BLAST of MC02g_new0122 vs. NCBI nr
Match: XP_022148438.1 (dual specificity protein kinase shkB isoform X1 [Momordica charantia])

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 564/585 (96.41%), Postives = 565/585 (96.58%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKKRFTLGV+LFVNQDAQEKFFKSKIESALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVVLFVNQDAQEKFFKSKIESALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNENLQIEMPLSTSSFA 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY
Sbjct: 301 VSVDEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRI IDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIGIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR
Sbjct: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 585

BLAST of MC02g_new0122 vs. NCBI nr
Match: KAG7010490.1 (Serine/threonine-protein kinase STY46 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 525/585 (89.74%), Postives = 547/585 (93.50%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDS RDPSPTK EGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPT+K TFACNSS+
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWS MSGALESAGIRCEKIQIFEKK+F+LGVILFVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSVMSGALESAGIRCEKIQIFEKKKFSLGVILFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGN  GKDLREIEEDAV+QNCRSGF+NSNLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLQIEMPLSTSSFT 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQ GGHELGKWLLSSENLEFTDQIGPNSFKGVYKG+RVAIEKIKGCEKG++Y
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR Q KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DV EGIKF+NDHGVAYRDLN QRILLDKNG+ACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PD+PKDCPSTLK+LMI+CWN CPSKRPQFSE+LS+LLDSNNNNH+
Sbjct: 541 PDVPKDCPSTLKALMIRCWNNCPSKRPQFSEVLSMLLDSNNNNHK 585

BLAST of MC02g_new0122 vs. NCBI nr
Match: XP_023512978.1 (mitogen-activated protein kinase kinase kinase 13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 523/585 (89.40%), Postives = 546/585 (93.33%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSK ESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKTESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDS RDPSPTK +GSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTDGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPT+K TFACNSS+
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKK+F+LGVILFVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVILFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGN  GKDLREIEEDAV+QNCRSGF+N+NLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENTNLNENLQIEMPLSTSSFT 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQ GGHELGKWLLSSENLEFTDQIGPNSFKGVYKG+RVAIEKIKGCEKG++Y
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR   KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLHMKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DV EGIKF+NDHGVAYRDLN QRILLDKNG+ACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PD+PKDCPSTLK+LMI+CWN CPSKRPQFSEILS+LLDSNNNNH+
Sbjct: 541 PDVPKDCPSTLKALMIRCWNNCPSKRPQFSEILSMLLDSNNNNHK 585

BLAST of MC02g_new0122 vs. NCBI nr
Match: XP_022986818.1 (fibroblast growth factor receptor [Cucurbita maxima])

HSP 1 Score: 1056 bits (2732), Expect = 0.0
Identity = 522/585 (89.23%), Postives = 544/585 (92.99%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           M TALECWSSRASTDEDLVEQVLMRTQDRSEGSK ESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   METALECWSSRASTDEDLVEQVLMRTQDRSEGSKSESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDS RDPSPTK EGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGF+MKDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPT+K TFACNSS+
Sbjct: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKK+F+LGVI FVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVIFFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGN  GKDLREIEEDAV+QNCRSGF+NSNLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLQIEMPLSTSSFT 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQ GGHELGKWLLSSENLEFTDQIGPNSFKGVYKG+RVAIEKIKGCEKG++Y
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR Q KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DV EGIKF+NDHGVAYRDLN QRILLDKNG+ACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PD+PKDCPSTLK+LM +CWN CPSKRPQFSEILS+LLDSNNNNH+
Sbjct: 541 PDVPKDCPSTLKALMTRCWNNCPSKRPQFSEILSMLLDSNNNNHK 585

BLAST of MC02g_new0122 vs. ExPASy TrEMBL
Match: A0A6J1D401 (dual specificity protein kinase shkB isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017085 PE=4 SV=1)

HSP 1 Score: 1136 bits (2939), Expect = 0.0
Identity = 564/566 (99.65%), Postives = 565/566 (99.82%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKKRFTLGV+LFVNQDAQEKFFKSKIESALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVVLFVNQDAQEKFFKSKIESALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKW 300
           TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKW
Sbjct: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNEWQTVQCGGHELGKW 300

Query: 301 LLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNI 360
           LLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNI
Sbjct: 301 LLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNI 360

Query: 361 LMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRIAIDVAEGIKFMNDHGV 420
           LMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRI IDVAEGIKFMNDHGV
Sbjct: 361 LMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEITRIGIDVAEGIKFMNDHGV 420

Query: 421 AYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNE 480
           AYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNE
Sbjct: 421 AYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNE 480

Query: 481 TWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCW 540
           TWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCW
Sbjct: 481 TWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCW 540

Query: 541 NTCPSKRPQFSEILSVLLDSNNNNHR 566
           NTCPSKRPQFSEILSVLLDSNNNNHR
Sbjct: 541 NTCPSKRPQFSEILSVLLDSNNNNHR 566

BLAST of MC02g_new0122 vs. ExPASy TrEMBL
Match: A0A6J1D436 (dual specificity protein kinase shkB isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017085 PE=4 SV=1)

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 564/585 (96.41%), Postives = 565/585 (96.58%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKKRFTLGV+LFVNQDAQEKFFKSKIESALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVVLFVNQDAQEKFFKSKIESALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNENLQIEMPLSTSSFA 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY
Sbjct: 301 VSVDEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRI IDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIGIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR
Sbjct: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 585

BLAST of MC02g_new0122 vs. ExPASy TrEMBL
Match: A0A6J1JHM8 (fibroblast growth factor receptor OS=Cucurbita maxima OX=3661 GN=LOC111484449 PE=4 SV=1)

HSP 1 Score: 1056 bits (2732), Expect = 0.0
Identity = 522/585 (89.23%), Postives = 544/585 (92.99%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           M TALECWSSRASTDEDLVEQVLMRTQDRSEGSK ESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   METALECWSSRASTDEDLVEQVLMRTQDRSEGSKSESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDS RDPSPTK EGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGF+MKDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPT+K TFACNSS+
Sbjct: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKK+F+LGVI FVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVIFFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGN  GKDLREIEEDAV+QNCRSGF+NSNLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLQIEMPLSTSSFT 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQ GGHELGKWLLSSENLEFTDQIGPNSFKGVYKG+RVAIEKIKGCEKG++Y
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR Q KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DV EGIKF+NDHGVAYRDLN QRILLDKNG+ACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PD+PKDCPSTLK+LM +CWN CPSKRPQFSEILS+LLDSNNNNH+
Sbjct: 541 PDVPKDCPSTLKALMTRCWNNCPSKRPQFSEILSMLLDSNNNNHK 585

BLAST of MC02g_new0122 vs. ExPASy TrEMBL
Match: A0A6J1FY74 (mitogen-activated protein kinase kinase kinase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449006 PE=4 SV=1)

HSP 1 Score: 1055 bits (2729), Expect = 0.0
Identity = 521/585 (89.06%), Postives = 545/585 (93.16%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDS RDPSPTK EGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYAQAGFE+KDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPT+K TFACNS +
Sbjct: 121 DSLPLSYAQAGFEIKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSLV 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALESAGIRCEKIQIFEKK+F+LGVILFVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVILFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           TNTVKLPFGFCGCQEGN  GKDLREIEEDAV+QNCRSGF+NSNLNE              
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLLIEMPLSTSSFT 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQTVQ GGHELGKWLLSSENLEFTDQIGPNSFKGVYKG+RVAIEKIKGCEKG++Y
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR Q KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRIA DV EGIKF+NDHGVAYRDLN QRILLDKNG+ACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAADVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PD+PKDCPS LK+LMI+CWN CPSKRPQFSE+LS++LDSNNN+H+
Sbjct: 541 PDVPKDCPSNLKALMIRCWNNCPSKRPQFSEVLSMVLDSNNNSHK 585

BLAST of MC02g_new0122 vs. ExPASy TrEMBL
Match: A0A0A0KC34 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G181580 PE=4 SV=1)

HSP 1 Score: 1033 bits (2670), Expect = 0.0
Identity = 509/585 (87.01%), Postives = 537/585 (91.79%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDED+VEQVLMRTQDRSEGSKP+SSFAVG KESSAM +RLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDMVEQVLMRTQDRSEGSKPDSSFAVGEKESSAMHRRLQRFSRNV 60

Query: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASLKNSLNLDS RDPSPT+ EGSKKAVWG+VVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSIRDPSPTRTEGSKKAVWGTVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180
           DSLPLSYA AGF+MKDVFLHIKL+EQASVYDHPAILFQEVTN D QKP +KFTFACNSS+
Sbjct: 121 DSLPLSYALAGFDMKDVFLHIKLMEQASVYDHPAILFQEVTNRDVQKPIIKFTFACNSSV 180

Query: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESALKLAIKKPK 240
           SWSAMSGALE+A IRCEKIQIFEKK+FTLGVILFVN D QEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALETAAIRCEKIQIFEKKKFTLGVILFVNLDLQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNE-------------- 300
           T  VKLPFGFCGCQEGN  GKDLRE EED VE NCRSGF+NSNL+E              
Sbjct: 241 TTAVKLPFGFCGCQEGNTGGKDLRETEEDGVEPNCRSGFENSNLSENLQIEMPLCTSSFA 300

Query: 301 -----WQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360
                WQT+Q GG+ELGKWLLSSENLEF DQ+GPNSFKGVYKG+RVAIEKIKGCEKG++Y
Sbjct: 301 VTVDEWQTIQSGGNELGKWLLSSENLEFVDQMGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK+ QTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKKLQTKEI 420

Query: 421 TRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480
           TRIAID+ EGIKFMNDHGVAYRDLN QRILLDKNG+ACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIAIDIVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YS GMVIWEMV+GEAAYGAYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSLGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 566
           PD+PKDC STLKSLMI+CWN CPSKRPQFSEILS+LLDSNNNNHR
Sbjct: 541 PDVPKDCSSTLKSLMIRCWNNCPSKRPQFSEILSLLLDSNNNNHR 585

BLAST of MC02g_new0122 vs. TAIR 10
Match: AT5G58520.1 (Protein kinase superfamily protein )

HSP 1 Score: 771.2 bits (1990), Expect = 5.9e-223
Identity = 381/599 (63.61%), Postives = 464/599 (77.46%), Query Frame = 0

Query: 1   MATALECWSSRA----STDEDLVEQVLMRTQDRSEG---SKPESSFAVGA------KESS 60
           MA ALECWSSRA      D DLV+QVLMRT DRSE    S PE+S  V        + SS
Sbjct: 1   MAAALECWSSRAGDGGDPDNDLVDQVLMRTHDRSESVITSLPETSLEVEGSTTVFDQSSS 60

Query: 61  AMQKRLQRLSRNVSEAVASLKNSLNLDSARD------PSPTKIE-----GSKKAVWGSVV 120
           AMQKR QRLSRNVSEA+ SLKN+LNLDSARD          K E     G +K VW +VV
Sbjct: 61  AMQKRFQRLSRNVSEAIVSLKNTLNLDSARDNQSFGGAMTPKAEVSGGGGGRKLVWATVV 120

Query: 121 RNLTQLYPGSQLPEKLVSNIRKHYDSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAIL 180
           +NL ++YPGSQLPEKLVSN++KHYDSLP SY+QA F+MK+VFLH+KLIEQA+  D+P  +
Sbjct: 121 KNLAKMYPGSQLPEKLVSNLKKHYDSLPFSYSQADFDMKEVFLHVKLIEQAAGDDNPVFM 180

Query: 181 FQEVTNHDTQKPTMKFTFACNSSISWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVN 240
            QEV+  + +   ++ TFACNS +SWS MSG L+SA I C+KIQIFEKK  TLGV+L ++
Sbjct: 181 IQEVSTEEPRGSVLRLTFACNSFLSWSTMSGVLDSASICCKKIQIFEKKGLTLGVVLLLD 240

Query: 241 QDAQEKFFKSKIESALKLAIKKPKTNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCR 300
           Q  Q   FK+++E+ LK+A KKPK  +VKLPFG CGCQE N    +L  +EE++++ + R
Sbjct: 241 QSGQHSLFKTRVENTLKVATKKPKPTSVKLPFGLCGCQEQNGGVGELGGVEEESIQHSSR 300

Query: 301 SGFDNSN-----------------LNEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFK 360
            G +N N                 ++EWQT+Q GG+E+GKWLL+S++ EF DQIGP S K
Sbjct: 301 LGIENLNSTIQIQVPLPSSSFAVSVDEWQTIQSGGNEIGKWLLNSDSFEFGDQIGPTSLK 360

Query: 361 GVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTK 420
           G+++GKRV IEK+KGC+KG +Y+FELRKD LELM CGHK+IL FYGVCIDENHGLCVVTK
Sbjct: 361 GIFRGKRVGIEKLKGCDKGNSYEFELRKDYLELMACGHKSILQFYGVCIDENHGLCVVTK 420

Query: 421 LMEGGSVHELMLKNKRFQTKEITRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSAC 480
           LMEGGS+HELMLKNK+ QTK+I RIAID+AEG+KF+NDHGVAYRDLN QRILLDK+G+AC
Sbjct: 421 LMEGGSLHELMLKNKKLQTKQILRIAIDIAEGLKFVNDHGVAYRDLNTQRILLDKHGNAC 480

Query: 481 LGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSG 540
           LG++GI+TACK+ GEA+EYETDGYRWLAPEIIAGDPE+  ETWMSNAYSFGMV+WEMV+G
Sbjct: 481 LGNIGIVTACKSFGEAVEYETDGYRWLAPEIIAGDPENTTETWMSNAYSFGMVLWEMVTG 540

Query: 541 EAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLL 559
           EAAY + SPVQAAVGIAACGLRP++PK+CP  L++LMI CWN  PSKRP FS I + LL
Sbjct: 541 EAAYASCSPVQAAVGIAACGLRPEIPKECPQVLRTLMINCWNNSPSKRPNFSHIHNTLL 599

BLAST of MC02g_new0122 vs. TAIR 10
Match: AT5G07140.1 (Protein kinase superfamily protein )

HSP 1 Score: 738.8 bits (1906), Expect = 3.3e-213
Identity = 363/583 (62.26%), Postives = 452/583 (77.53%), Query Frame = 0

Query: 1   MATALECWSSR----ASTDEDLVEQVLMRTQDRSEG--SKPESSFAVGAKESSAMQKRLQ 60
           MA+ALECW++R       D+D V+QVLM ++DRSE   + P S      + SSAMQKR Q
Sbjct: 1   MASALECWTTRNAAGGCADDDFVDQVLMSSEDRSESLTAPPPSD-----QTSSAMQKRFQ 60

Query: 61  RLSRNVSEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVS 120
           RL RNVS+A+ASLKNSLNLDS+RD       G +K VW +VVRNL ++YPGSQLP+KLVS
Sbjct: 61  RLGRNVSDAIASLKNSLNLDSSRDNQNAATGGGRKLVWATVVRNLAKMYPGSQLPDKLVS 120

Query: 121 NIRKHYDSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNH--DTQKPTMKF 180
           N+RKHYDSLPLSY+Q GF+MKDVF+HIKLIEQA   D+P  + QEV +   D Q    K 
Sbjct: 121 NLRKHYDSLPLSYSQTGFDMKDVFVHIKLIEQAFGDDNPVFVIQEVCDEEADDQGSVFKL 180

Query: 181 TFACNSSISWSAMSGALESAGIRCEKIQIFEKKRFTLGVILFVNQDAQEKFFKSKIESAL 240
           TFAC  S+ WS +SG+L+ A I C+K+QIFEKK  TLGV+L + +  QEK FK K+ +AL
Sbjct: 181 TFACTCSLPWSTISGSLDGALICCKKVQIFEKKGLTLGVVLLLVESGQEKMFKVKVVNAL 240

Query: 241 KLAIKKPKTNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSN--------- 300
           + A++KPK+ +VKLPFG CGC+E NA   +  +++ ++++Q  R   D+ N         
Sbjct: 241 RSAVRKPKSTSVKLPFGLCGCEEQNAGVGEFGDVDVESIDQCYRHELDDLNTRIQLQMPP 300

Query: 301 --------LNEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGC 360
                   ++EWQT+Q GG ++ KWLL+S++LEF+ Q+GPNSFKGVY+G +VAIEK+KGC
Sbjct: 301 PSSSFSVSVDEWQTIQSGGDDIRKWLLNSDDLEFSGQLGPNSFKGVYRGTKVAIEKLKGC 360

Query: 361 EKGIAYKFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR 420
           EKG +Y+F +RKD LELMTCGHK+IL FYGVCIDENHGLCVVTKLM+GGS+ EL+LK K+
Sbjct: 361 EKGNSYEFAIRKDFLELMTCGHKSILQFYGVCIDENHGLCVVTKLMQGGSLRELVLKKKK 420

Query: 421 FQTKEITRIAIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEA 480
            QTK I +IA+D+AEG+KF+NDHGVAYRDLN QRILLDK  +ACLGD+GI+TACK++ EA
Sbjct: 421 LQTKLIFQIAVDIAEGMKFINDHGVAYRDLNTQRILLDKQCNACLGDLGIVTACKSVNEA 480

Query: 481 MEYETDGYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGI 540
           MEYETDGYRWLAPEIIAGDPE   E+WMSNAYSFGMV+WEMV+GE AYG+ SPVQAAVGI
Sbjct: 481 MEYETDGYRWLAPEIIAGDPEKTRESWMSNAYSFGMVLWEMVTGEEAYGSCSPVQAAVGI 540

Query: 541 AACGLRPDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLL 559
           AACGLRPD+PK+CP  LK LMIKCWNTCPS R  FS+I  +LL
Sbjct: 541 AACGLRPDIPKECPQVLKYLMIKCWNTCPSTRLNFSQIHCILL 578

BLAST of MC02g_new0122 vs. TAIR 10
Match: AT4G38470.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 163.7 bits (413), Expect = 4.3e-40
Identity = 86/263 (32.70%), Postives = 149/263 (56.65%), Query Frame = 0

Query: 300 WLLSSENLEFTDQIGPNSFKGVYKG----KRVAIEKIKGCEKGIAYKFELRKDLLELMTC 359
           W ++ ++L+F  +I   S+  +YKG    + VAI+ +K        + E  +++  +   
Sbjct: 283 WEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFIMRKV 342

Query: 360 GHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR-FQTKEITRIAIDVAEGIKF 419
            HKN++ F G C    H LC+VT+ M GGSV++ + K K  F+   + ++AID+ +G+ +
Sbjct: 343 RHKNVVQFIGACTKPPH-LCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKGMSY 402

Query: 420 MNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGD 479
           ++ + + +RDL A  +L+D+N    + D G+       G  M  ET  YRW+APE+I   
Sbjct: 403 LHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTG-VMTAETGTYRWMAPEVIEHK 462

Query: 480 PESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLKS 539
           P        ++ +S+G+V+WE+++G+  Y   +P+QAAVG+   GLRP +PK+    L  
Sbjct: 463 PYD----HKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAE 522

Query: 540 LMIKCWNTCPSKRPQFSEILSVL 558
           L+ + W    ++RP FSEI+  L
Sbjct: 523 LLERLWEHDSTQRPDFSEIIEQL 539

BLAST of MC02g_new0122 vs. TAIR 10
Match: AT4G35780.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 160.2 bits (404), Expect = 4.8e-39
Identity = 81/264 (30.68%), Postives = 147/264 (55.68%), Query Frame = 0

Query: 299 KWLLSSENLEFTDQIGPNS----FKGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMT 358
           +W +  + L+   ++   S    F+G Y  + VAI+ +K          E  +++  +  
Sbjct: 284 EWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRK 343

Query: 359 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR-FQTKEITRIAIDVAEGIK 418
             HKN++ F G C   +  LC+VT+ M  GS+++ + K+K  F+ + + ++A+DV++G+ 
Sbjct: 344 VRHKNVVQFIGAC-TRSPNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMN 403

Query: 419 FMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAG 478
           +++ + + +RDL    +L+D++    + D G+       G  M  ET  YRW+APE+I  
Sbjct: 404 YLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESG-VMTAETGTYRWMAPEVIEH 463

Query: 479 DPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLK 538
            P        ++ +S+ +V+WE+++GE  Y   +P+QAAVG+   GLRP +PK+    L 
Sbjct: 464 KPYD----HRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLT 523

Query: 539 SLMIKCWNTCPSKRPQFSEILSVL 558
            L+ KCW   P+ RP F+EI+ +L
Sbjct: 524 ELLEKCWQQDPALRPNFAEIIEML 541

BLAST of MC02g_new0122 vs. TAIR 10
Match: AT2G17700.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 159.1 bits (401), Expect = 1.1e-38
Identity = 80/264 (30.30%), Postives = 147/264 (55.68%), Query Frame = 0

Query: 299 KWLLSSENLEFTDQIGPNSF----KGVYKGKRVAIEKIKGCEKGIAYKFELRKDLLELMT 358
           +W +    L+   ++   S+    +G Y  + VAI+ +K          E  +++  +  
Sbjct: 278 EWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRK 337

Query: 359 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK-RFQTKEITRIAIDVAEGIK 418
             HKN++ F G C   +  LC+VT+ M  GS+++ + K K  F+ + + ++A+DVA+G+ 
Sbjct: 338 VRHKNVVQFLGAC-TRSPTLCIVTEFMARGSIYDFLHKQKCAFKLQTLLKVALDVAKGMS 397

Query: 419 FMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAG 478
           +++ + + +RDL    +L+D++G   + D G+       G  M  ET  YRW+APE+I  
Sbjct: 398 YLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESG-VMTAETGTYRWMAPEVIEH 457

Query: 479 DPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLRPDLPKDCPSTLK 538
            P +      ++ +S+ +V+WE+++G+  Y   +P+QAAVG+   GLRP +PK     +K
Sbjct: 458 KPYN----HKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPKVK 517

Query: 539 SLMIKCWNTCPSKRPQFSEILSVL 558
            L+ +CW+  P +RP F EI+ +L
Sbjct: 518 GLLERCWHQDPEQRPLFEEIIEML 535

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JTP56.1e-3932.70Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
Q8RWL66.7e-3830.68Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
O225581.5e-3730.30Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
Q7TQP61.1e-3232.34Serine/threonine-protein kinase TNNI3K OS=Rattus norvegicus OX=10116 GN=Tnni3k P... [more]
Q5GIG69.4e-3231.60Serine/threonine-protein kinase TNNI3K OS=Mus musculus OX=10090 GN=Tnni3k PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_022148439.10.099.65dual specificity protein kinase shkB isoform X2 [Momordica charantia][more]
XP_022148438.10.096.41dual specificity protein kinase shkB isoform X1 [Momordica charantia][more]
KAG7010490.10.089.74Serine/threonine-protein kinase STY46 [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_023512978.10.089.40mitogen-activated protein kinase kinase kinase 13 [Cucurbita pepo subsp. pepo][more]
XP_022986818.10.089.23fibroblast growth factor receptor [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1D4010.099.65dual specificity protein kinase shkB isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1D4360.096.41dual specificity protein kinase shkB isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1JHM80.089.23fibroblast growth factor receptor OS=Cucurbita maxima OX=3661 GN=LOC111484449 PE... [more]
A0A6J1FY740.089.06mitogen-activated protein kinase kinase kinase 13 OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0KC340.087.01Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G181... [more]
Match NameE-valueIdentityDescription
AT5G58520.15.9e-22363.61Protein kinase superfamily protein [more]
AT5G07140.13.3e-21362.26Protein kinase superfamily protein [more]
AT4G38470.14.3e-4032.70ACT-like protein tyrosine kinase family protein [more]
AT4G35780.14.8e-3930.68ACT-like protein tyrosine kinase family protein [more]
AT2G17700.11.1e-3830.30ACT-like protein tyrosine kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 528..550
score: 43.81
coord: 484..506
score: 26.95
coord: 378..391
score: 22.66
coord: 414..432
score: 29.06
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 326..556
e-value: 6.5E-40
score: 137.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 300..563
e-value: 7.8E-51
score: 174.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..46
NoneNo IPR availablePANTHERPTHR23257:SF765PROTEIN KINASE SUPERFAMILY PROTEINcoord: 1..560
NoneNo IPR availablePANTHERPTHR23257SERINE-THREONINE PROTEIN KINASEcoord: 1..560
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 307..566
score: 26.025654
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 294..557

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g_new0122.1MC02g_new0122.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity