MC02g_new0107 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g_new0107
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRNase H domain-containing protein
LocationMC02: 4886640 .. 4887673 (-)
RNA-Seq ExpressionMC02g_new0107
SyntenyMC02g_new0107
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCATTTACTGGTGTCAATTTGGAGGCTCGTGGTGTGGTTGGTGTTAACTGGTGTGGCTTATGTGGTCGGTCTCGTGAGGATCTCCTCCATACGCTCTGGTTTTGTAAAGTGGCTGTTCGAGTCTGGAAGGCGTCCAGGCTCCGGTACCAATTACCGCAGGTGGTAGGGTCGACGATTATGACTTTCTCATAGGATTGTAAGGAGGTTTTGGATTGGGATGATATGAGAGAGCTAGTTGTGATCCTTTGGGCTATTTGGAGTTTGAGGAACGATTGGGTTTTCAATAAGGTGAGAAGGTGTGTTCTGGAGGATATTGGAAGCTGGGCAACGGCTTTTGTGCAAGAATTTTATCGGGCAAACGCTCGTACTCTGGATAACGTTTCTTGTCATGTTTCTACTCTTTCGCCTGTTTGTTGGAGACCTCCTCCGGAGGGTCGGTACACAATTAATACCGATGCTGCTGTGGATTTGAACTCGTTTTCCGCTGGTCTAGGTGTGGTTGTCAGAGATTGTCGGGGTCAGGTGATGCTCCCAGCTGCAACAAGGGTGTTGGATATGGCAAGTGTTGATCTAGCAGAGGCGTCGACGGCTAAATGGGGTCTTGATCTGGCACGGGATATGGGTCTCAGTCCTTGCGTTCTAGAGATAGTCTCCCTTCGGATTGTCCAATCGTTGCAGTCGATTGAGGAGGACCCCTGTCGGAAATGGGGTGTTTGTGACGAAATTAAAGGAAGCAGCTGCGGATCTCGGCCTTGCATCTGTTAGTTTTGTGAAGAGAGGTAAAAACCAAGTGGCTCATTTACTTGCGGGTTTGGGGTTGAAGAATGGGGGTGTGTCGGTTTGGTTAGAGGATGGTTCAGATATTATTTCTCATGTATTGCTCCTTGAGAGTTTTGGTGTTTCTGCTTAATTGATGACTTTAGCTCGTTAAAAAAAATAGTATTACTAATTTAGATATTTTCTTAGTATAATAAATGTGGATGTATCAAAATTTGAACTACCAAATGAAGAAGTTCTCTATTGAATGGGGTAG

mRNA sequence

ATGAGAGAGCTAGTTGTGATCCTTTGGGCTATTTGGAGTTTGAGGAACGATTGGGTTTTCAATAAGGTGAGAAGGTGTGTTCTGGAGGATATTGGAAGCTGGGCAACGGCTTTTGTGCAAGAATTTTATCGGGCAAACGCTCGTACTCTGGATAACGTTTCTTGTCATGTTTCTACTCTTTCGCCTGTTTGTTGGAGACCTCCTCCGGAGGGTCGGTACACAATTAATACCGATGCTGCTGTGGATTTGAACTCGTTTTCCGCTGGTCTAGGTGTGGTTGTCAGAGATTGTCGGGGTCAGGTGATGCTCCCAGCTGCAACAAGGGTGTTGGATATGGCAAGTGTTGATCTAGCAGAGGCGTCGACGGCTAAATGGGGTCTTGATCTGGCACGGGATATGGGTCTCAGTCCTTGCGTTCTAGAGATAGTCTCCCTTCGGATTGTCCAATCGTTGCAGTCGATTGAGGAGGACCCCTGTCGGAAATGGGGTGTTTGTGACGAAATTAAAGGAAGCAGCTGCGGATCTCGGCCTTGCATCTGTTAG

Coding sequence (CDS)

ATGAGAGAGCTAGTTGTGATCCTTTGGGCTATTTGGAGTTTGAGGAACGATTGGGTTTTCAATAAGGTGAGAAGGTGTGTTCTGGAGGATATTGGAAGCTGGGCAACGGCTTTTGTGCAAGAATTTTATCGGGCAAACGCTCGTACTCTGGATAACGTTTCTTGTCATGTTTCTACTCTTTCGCCTGTTTGTTGGAGACCTCCTCCGGAGGGTCGGTACACAATTAATACCGATGCTGCTGTGGATTTGAACTCGTTTTCCGCTGGTCTAGGTGTGGTTGTCAGAGATTGTCGGGGTCAGGTGATGCTCCCAGCTGCAACAAGGGTGTTGGATATGGCAAGTGTTGATCTAGCAGAGGCGTCGACGGCTAAATGGGGTCTTGATCTGGCACGGGATATGGGTCTCAGTCCTTGCGTTCTAGAGATAGTCTCCCTTCGGATTGTCCAATCGTTGCAGTCGATTGAGGAGGACCCCTGTCGGAAATGGGGTGTTTGTGACGAAATTAAAGGAAGCAGCTGCGGATCTCGGCCTTGCATCTGTTAG

Protein sequence

MRELVVILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRANARTLDNVSCHVSTLSPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAEASTAKWGLDLARDMGLSPCVLEIVSLRIVQSLQSIEEDPCRKWGVCDEIKGSSCGSRPCIC
Homology
BLAST of MC02g_new0107 vs. NCBI nr
Match: XP_022149385.1 (uncharacterized protein LOC111017816 [Momordica charantia])

HSP 1 Score: 165 bits (417), Expect = 9.45e-44
Identity = 90/136 (66.18%), Postives = 95/136 (69.85%), Query Frame = 0

Query: 1   MRELVVILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRANARTLDNVSCHVSTL 60
           MREL VILWAIWSLRNDWVFNKV R V EDIGSWA AF+QEF RANARTLD++SCHVS  
Sbjct: 601 MRELAVILWAIWSLRNDWVFNKVSRHVPEDIGSWAAAFMQEFRRANARTLDDISCHVSA- 660

Query: 61  SPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAEA 120
           S  C      GR                       DCRGQVML AATRVLDMA+VDLAEA
Sbjct: 661 SFACLLETSSGR-----------------------DCRGQVMLSAATRVLDMANVDLAEA 712

Query: 121 STAKWGLDLARDMGLS 136
           S AKWGLDLARDMGL+
Sbjct: 721 SAAKWGLDLARDMGLN 712

BLAST of MC02g_new0107 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 98.6 bits (244), Expect = 1.11e-21
Identity = 63/157 (40.13%), Postives = 87/157 (55.41%), Query Frame = 0

Query: 2   RELVVILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRAN---ARTLDNVSCHVS 61
            ELVV LW++W+ RN +VFNK RR   +D+  W + ++  F   N   A    +VS    
Sbjct: 18  EELVVFLWSLWNRRNAFVFNK-RRVEDDDLAGWVSTYIATFKATNTNHATANQDVSQSFQ 77

Query: 62  TLSPV-------CWRPPPEGRYTINTDAAVDLNSFSAGLGVVV-RDCRGQVMLPAATRVL 121
             S +        W P  EG + + TDA+     F+AGLGV++ RD RGQV+  A   + 
Sbjct: 78  QSSQIHQAQNHTIWCPAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLE 137

Query: 122 DMASVDLAEASTAKWGLDLARDMGLSPCVLEIVSLRI 147
            +ASVD AEA  A  GL +A + G+SP +LE  SLRI
Sbjct: 138 HVASVDDAEALAAVEGLRVAMETGISPILLETDSLRI 173

BLAST of MC02g_new0107 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 97.4 bits (241), Expect = 9.28e-20
Identity = 50/144 (34.72%), Postives = 81/144 (56.25%), Query Frame = 0

Query: 2    RELVVILWAIWSLRNDWVFNKVRRCVLE---DIGSWATAFVQEFYRANARTLDNVSCHVS 61
             EL V++W +W+ RN   FN   + V +   ++  WA  +  EF  A +  +      V+
Sbjct: 928  EELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITG---RVT 987

Query: 62   TLSPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLA 121
              + + W+PP EG Y INTDA+   +   AGLG+++ + RGQVM  A   + ++ SVD+A
Sbjct: 988  NTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMA 1047

Query: 122  EASTAKWGLDLARDMGLSPCVLEI 142
            EA  A  GL LA ++G+ P + ++
Sbjct: 1048 EAIAAVEGLQLASEIGMHPALEDL 1068

BLAST of MC02g_new0107 vs. NCBI nr
Match: XP_030974621.1 (uncharacterized protein LOC115994555 [Quercus lobata])

HSP 1 Score: 91.3 bits (225), Expect = 3.31e-19
Identity = 56/153 (36.60%), Postives = 78/153 (50.98%), Query Frame = 0

Query: 6   VILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRANARTLDNVSCHVSTLSPVCW 65
           +I WAIWS RN+ V N  R  V+   G W T +++E+Y AN +        V T     W
Sbjct: 15  IIAWAIWSNRNE-VCNGGR--VVHGSGRWTTRYLKEYYAANEQL-----AIVPTAQITNW 74

Query: 66  RPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAEASTAKW 125
            PPP  RY IN D  V  +   AG+GV+VRDC GQV+   + ++         EA   + 
Sbjct: 75  IPPPTNRYKINVDGVVFKSQKIAGVGVMVRDCCGQVIAALSKKINVPLGPLETEAKAVEV 134

Query: 126 GLDLARDMGLSPCVLEIVSLRIVQSLQSIEEDP 158
           G+  ARD+G+  C++E  SL I  +L      P
Sbjct: 135 GVQFARDIGIQDCIIEGDSLTIYTALYGNTSPP 159

BLAST of MC02g_new0107 vs. NCBI nr
Match: XP_023917764.1 (uncharacterized protein LOC112029308 [Quercus suber])

HSP 1 Score: 90.5 bits (223), Expect = 1.37e-18
Identity = 56/172 (32.56%), Postives = 86/172 (50.00%), Query Frame = 0

Query: 7   ILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRANARTLDNVSCHVSTLSPVCWR 66
           I W++W  RN  +        L +IG+ A   V EF+ A+    + VSC +       W 
Sbjct: 22  IAWSLWERRNR-IRENQPSWPLHEIGNRAKKMVVEFFDAH----EQVSCSLPRSPWARWA 81

Query: 67  PPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAEASTAKWG 126
           PPP G Y  N DAA+       G+GVV+RDC G+++   + ++L   SV+ AEA  A   
Sbjct: 82  PPPAGSYKANFDAALFEERNFGGIGVVIRDCMGEIIGALSQKILLPQSVEHAEALAASRA 141

Query: 127 LDLARDMGLSPCVLEIVSLRIVQSLQSIEEDPCRKWGVCDEIKGSSCGSRPC 178
           + LA ++ L   + E  SLR++ ++ S E      W + +EI+G S     C
Sbjct: 142 VQLATELCLFDVIFEGDSLRVITAINSAEACRAMFWHIVEEIRGRSLSLARC 188

BLAST of MC02g_new0107 vs. ExPASy TrEMBL
Match: A0A6J1D5K1 (uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017816 PE=4 SV=1)

HSP 1 Score: 165 bits (417), Expect = 4.58e-44
Identity = 90/136 (66.18%), Postives = 95/136 (69.85%), Query Frame = 0

Query: 1   MRELVVILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRANARTLDNVSCHVSTL 60
           MREL VILWAIWSLRNDWVFNKV R V EDIGSWA AF+QEF RANARTLD++SCHVS  
Sbjct: 601 MRELAVILWAIWSLRNDWVFNKVSRHVPEDIGSWAAAFMQEFRRANARTLDDISCHVSA- 660

Query: 61  SPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAEA 120
           S  C      GR                       DCRGQVML AATRVLDMA+VDLAEA
Sbjct: 661 SFACLLETSSGR-----------------------DCRGQVMLSAATRVLDMANVDLAEA 712

Query: 121 STAKWGLDLARDMGLS 136
           S AKWGLDLARDMGL+
Sbjct: 721 SAAKWGLDLARDMGLN 712

BLAST of MC02g_new0107 vs. ExPASy TrEMBL
Match: A0A6J1CDQ4 (uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010533 PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 5.38e-22
Identity = 63/157 (40.13%), Postives = 87/157 (55.41%), Query Frame = 0

Query: 2   RELVVILWAIWSLRNDWVFNKVRRCVLEDIGSWATAFVQEFYRAN---ARTLDNVSCHVS 61
            ELVV LW++W+ RN +VFNK RR   +D+  W + ++  F   N   A    +VS    
Sbjct: 18  EELVVFLWSLWNRRNAFVFNK-RRVEDDDLAGWVSTYIATFKATNTNHATANQDVSQSFQ 77

Query: 62  TLSPV-------CWRPPPEGRYTINTDAAVDLNSFSAGLGVVV-RDCRGQVMLPAATRVL 121
             S +        W P  EG + + TDA+     F+AGLGV++ RD RGQV+  A   + 
Sbjct: 78  QSSQIHQAQNHTIWCPAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHRGQVLASATKYLE 137

Query: 122 DMASVDLAEASTAKWGLDLARDMGLSPCVLEIVSLRI 147
            +ASVD AEA  A  GL +A + G+SP +LE  SLRI
Sbjct: 138 HVASVDDAEALAAVEGLRVAMETGISPILLETDSLRI 173

BLAST of MC02g_new0107 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.49e-20
Identity = 50/144 (34.72%), Postives = 81/144 (56.25%), Query Frame = 0

Query: 2    RELVVILWAIWSLRNDWVFNKVRRCVLE---DIGSWATAFVQEFYRANARTLDNVSCHVS 61
             EL V++W +W+ RN   FN   + V +   ++  WA  +  EF  A +  +      V+
Sbjct: 928  EELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWANKYAMEFREAKSNPITG---RVT 987

Query: 62   TLSPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLA 121
              + + W+PP EG Y INTDA+   +   AGLG+++ + RGQVM  A   + ++ SVD+A
Sbjct: 988  NTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRGQVMAAATKYLENIQSVDMA 1047

Query: 122  EASTAKWGLDLARDMGLSPCVLEI 142
            EA  A  GL LA ++G+ P + ++
Sbjct: 1048 EAIAAVEGLQLASEIGMHPALEDL 1068

BLAST of MC02g_new0107 vs. ExPASy TrEMBL
Match: A0A5C7IIT4 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_004125 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.95e-19
Identity = 54/153 (35.29%), Postives = 79/153 (51.63%), Query Frame = 0

Query: 4   LVVILWAIWSLRNDWVFNKVRRCVLE-DIGSWATAFVQEFYRANARTLDNVSCHVSTLSP 63
           L V+ W +W  RN  V+ K  + V + D+  WA +F+Q+F  A  +T+D  S     ++P
Sbjct: 643 LCVVWWRVWYRRNQLVYEKSSQTVHDFDVLDWAASFIQDFKAA--KTVDTGSVVKQRVAP 702

Query: 64  VCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAEAST 123
             W+P P G Y INTDA +D  +   G+GVV+RDC G VM         +      EA  
Sbjct: 703 K-WKPSPSGSYKINTDATLDCRAKVTGIGVVIRDCYGHVMASLCQSFPGLLQPQTVEAVA 762

Query: 124 AKWGLDLARDMGLSPCVLEIVSLRIVQSLQSIE 155
              G  LA + GL P  +E  SL +V  + S++
Sbjct: 763 VLRGFRLALEAGLCPASIESDSLSVVNLINSMD 792

BLAST of MC02g_new0107 vs. ExPASy TrEMBL
Match: A0A6J1CIF1 (uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011237 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 3.68e-17
Identity = 49/124 (39.52%), Postives = 68/124 (54.84%), Query Frame = 0

Query: 34  WATAFVQEFYRANARTLDNVSCHVSTLSPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVV 93
           WA  +V EF  AN+         V+  + V W PP +  Y INTDA+   +   AGLG++
Sbjct: 6   WANKYVMEFREANSNPFPG---RVTNTAEVLWLPPDKRIYKINTDASFLASDQHAGLGII 65

Query: 94  VRDCRGQVMLPAATRVLDMASVDLAEASTAKWGLDLARDMGLSPCVLEIVSLRIVQSLQS 153
           +R+ RGQVM  A   + ++ SVD+AEA  A  GL LA  +G++P +LE  S RI      
Sbjct: 66  IRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQ 125

Query: 154 IEED 157
             ED
Sbjct: 126 PSED 126

BLAST of MC02g_new0107 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 1.3e-06
Identity = 39/133 (29.32%), Postives = 59/133 (44.36%), Query Frame = 0

Query: 7   ILWAIWSLRNDWVF-------NKVRRCVLEDIGSWATAFVQEFYRANARTLDNVSCHVST 66
           +LW +W  RN+ VF        +V R   +D+  W         R  A +       V+ 
Sbjct: 363 LLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWR-------IRTEAESC-GTKPQVNR 422

Query: 67  LSPVCWRPPPEGRYTINTDAAVDLNSFSAGLGVVVRDCRGQVMLPAATRVLDMASVDLAE 126
            S   WRPPP      NTDA  + ++   G+G V+R+ +G+V    A  +  + SV  AE
Sbjct: 423 SSCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAE 482

Query: 127 ASTAKWG-LDLAR 132
               +W  L L+R
Sbjct: 483 LEAMRWAVLSLSR 487

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022149385.19.45e-4466.18uncharacterized protein LOC111017816 [Momordica charantia][more]
XP_022139684.11.11e-2140.13uncharacterized protein LOC111010533 [Momordica charantia][more]
XP_022150918.19.28e-2034.72uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_030974621.13.31e-1936.60uncharacterized protein LOC115994555 [Quercus lobata][more]
XP_023917764.11.37e-1832.56uncharacterized protein LOC112029308 [Quercus suber][more]
Match NameE-valueIdentityDescription
A0A6J1D5K14.58e-4466.18uncharacterized protein LOC111017816 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1CDQ45.38e-2240.13uncharacterized protein LOC111010533 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1DAR44.49e-2034.72uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A5C7IIT46.95e-1935.29Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_004125 PE=4 SV=1[more]
A0A6J1CIF13.68e-1739.52uncharacterized protein LOC111011237 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
Match NameE-valueIdentityDescription
AT4G29090.11.3e-0629.32Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 76..156
e-value: 1.4E-11
score: 44.3
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 4..157
NoneNo IPR availablePANTHERPTHR47074:SF3OS07G0513450 PROTEINcoord: 4..157
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 75..169
e-value: 4.58061E-10
score: 52.7016

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g_new0107.1MC02g_new0107.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity