MC02g_new0003 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g_new0003 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 2.1e-262
Identity = 407/547 (74.41%), Postives = 469/547 (85.74%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSN 64
           +    L L+AAA   V  EDPYF   WN+TYGT SPLGVPQQ ILINGQFPGPNINSTSN
Sbjct: 6   LLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSN 65

Query: 65  NNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
           NN+++NVFNNLDEPFL+ W+GIQHRKN WQDG  GT CPI PGTNYTYHFQ KDQIGS+F
Sbjct: 66  NNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYF 125

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRS 184
           YYP+T MHRAAGG+GGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSH+ LK FLD GR+
Sbjct: 126 YYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRT 185

Query: 185 LARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVEME 244
           + RPDG++INGK+ KGDG+D PLFT+KP KTY+ R+CN+G+KTS+N R Q+H MKLVEME
Sbjct: 186 IGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEME 245

Query: 245 GSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGP 304
           GSHV+QNDY SLD+HVGQCF  +VTANQEPKDYY++AS+RF K V+   G++RY  GKGP
Sbjct: 246 GSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGP 305

Query: 305 ASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVD 364
           AS ++P  PVGWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  KVD
Sbjct: 306 ASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 365

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNATF 424
           GKLR+A+NGVSH +PETPLKLAEYFGI+DKVFKYDTI+DD   E    + + PNV+N T 
Sbjct: 366 GKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITH 425

Query: 425 RNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFPKS 484
           R FVE+VFENHEKS+QSWHL+GYSFF+VA+EPG WTPEKRKNYNLLDAVSR T+QV+PK 
Sbjct: 426 RTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 485

Query: 485 WAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 544
           WAAILLT DN GMWN+RSE TE RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK+ 
Sbjct: 486 WAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNT 545

Query: 545 PLP-KPY 551
           P P  PY
Sbjct: 546 PKPVNPY 552

BLAST of MC02g_new0003 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 8.7e-240
Identity = 379/547 (69.29%), Postives = 451/547 (82.45%), Query Frame = 0

Query: 8   VLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNI 67
           +LLCLS   ++    EDPY +F WN+TYGTI+PLGVPQQGILINGQFPGP IN TSNNNI
Sbjct: 11  LLLCLSVGVIA----EDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNI 70

Query: 68  VVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYP 127
           VVNVFNNLDEPFL  W+G+QHRKNSWQDG  GT CPI PG N+TY FQVKDQIGS+ Y+P
Sbjct: 71  VVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFP 130

Query: 128 STAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRSLAR 187
           +TA+HRAAGG+G L V+SR LIPVP+ +P D+Y V VGDWY K H  LK  LD GR++ R
Sbjct: 131 TTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGR 190

Query: 188 PDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVEMEGSH 247
           PDG++INGK+AK     EPLFTM+  KTY+YR CN+G+++S+NIRFQ H MKLVE+EGSH
Sbjct: 191 PDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSH 250

Query: 248 VVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGPASP 307
            VQN Y SLD+HVGQC SVLVTA+QEPKDYYL+ S+RF K  L    IIRY NGKGPASP
Sbjct: 251 TVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASP 310

Query: 308 EIPEAP----VGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKV 367
           E+P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIKI NS ++V
Sbjct: 311 ELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQV 370

Query: 368 DGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNAT 427
            GKLRY +NG+SH + ETPLKL EYFG  +K FKYD ++D+ PA+  + +T+A NV NAT
Sbjct: 371 GGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPAD-PSKLTIATNVKNAT 430

Query: 428 FRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFPK 487
           +RNFVEI+FENHEK+++++HL+GYSFFAVA+EPGRW+PEKRKNYNL+D +SR+ IQV+P 
Sbjct: 431 YRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPN 490

Query: 488 SWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKD 547
           SWAAI+LT DNAGMWNLRSE+ E  YLG+QLYFSVLSP+RSLRDEYNIPDN  LCG+VK 
Sbjct: 491 SWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 550

Query: 548 MPLPKPY 551
           + +P PY
Sbjct: 551 LSMPAPY 552

BLAST of MC02g_new0003 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 4.9e-158
Identity = 271/562 (48.22%), Postives = 368/562 (65.48%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSN 64
           +F +LL +    +S     DPY F+ + ++Y T SPLGVPQQ I ING+FPGP IN T+N
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 65  NNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
            N+VVNV N LDE  L+HW+GIQ R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFF
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRS 184
           Y+PS    RA+GGFG   VN R +IPVP++ P+ D TV +GDWY ++H+AL+  LD G+ 
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 185 LARPDGVLINGK-------NAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHT 244
           L  PDGVLINGK           DG D    T+ P KTY+ RV N+G+ TSLN R Q H 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 245 MKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQE-PKDYYLIASTRFTKNVL----VG 304
           + L E EGS+ VQ +Y SLDIHVGQ +S LVT +Q    DYY++AS R     +     G
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 305 KGIIRYTNGKGPASPEIPEAP---VGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSIN 364
            GI++YTN KG A  ++P  P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 365 ITRTIKIIN-SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD--DGP 424
           +T    + N     + GK R  +NG+S  +P TP++LA+   + D V+K D       GP
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLDFPKRPLTGP 422

Query: 425 AEGANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKN 484
           A+      VA +++N T+R F+E+V +N++  +QS+H++GY+FF V ++ G WT   R  
Sbjct: 423 AK------VATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGT 482

Query: 485 YNLLDAVSRDTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLR 544
           YN  D ++R TIQV+P +W+AIL++LDN G WNLR+E  ++ YLGQ+ Y  V++P  + +
Sbjct: 483 YNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNK 542

Query: 545 DEYNIPDNTLLCGLVKDMPLPK 549
            E+  PDN L CG +  +  P+
Sbjct: 543 TEFGHPDNVLYCGALSKLQKPQ 557

BLAST of MC02g_new0003 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 4.9e-150
Identity = 249/539 (46.20%), Postives = 367/539 (68.09%), Query Frame = 0

Query: 24  DPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSNNNIVVNVFNNLDEPFLIHW 83
           DPY  + + ++Y T SPLGVPQQ I +NG+FPGP IN+T+N N+ VNV N+LDEP L+ W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 84  SGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYPSTAMHRAAGGFGGLRV 143
            G+Q R+NSWQDG+LGTNCPIPP  N+TY FQ+KDQIGS+FY PS    RA+GGFG L +
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 144 NSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRSLARPDGVLINGK------N 203
           N+R L+P+P+ +P+ +   ++GDWYT++H+AL+  LDSG+ L  PDGVLINGK      +
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 204 AKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVEMEGSHVVQNDYQSLD 263
           +  DG +     + P KTY+ RV N+G+ TSLN R Q+H + L+E EG +  Q ++   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 264 IHVGQCFSVLVTANQE-PKDYYLIASTRFTKNV----LVGKGIIRYTNGKGPASPEIP-- 323
           +HVGQ +S LVT +Q    DYY++AS RF        + G GI+ Y+N KGPAS  +P  
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 324 EAPVGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS--ATKVDGKL 383
              V   WS +NQ R  + N +AS ARPNPQGS+HYG INITRT  I+ S   TK++GKL
Sbjct: 325 ATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTY-ILRSLPPTKINGKL 384

Query: 384 RYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD--DGPAEGANTVTVAPNVVNATFR 443
           R  +NG+S ++P TP++LA+     D   K D + D  D P +      ++ +++NAT++
Sbjct: 385 RATLNGISFVNPSTPMRLAD-----DHKVKGDYMLDFPDRPLD-EKLPRLSSSIINATYK 444

Query: 444 NFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFPKSW 503
            F++++F+N++  +QS+H++GY+F+ VA++ G W+ ++  +YN  DAV+R T++V+P +W
Sbjct: 445 GFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAW 504

Query: 504 AAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 545
            A+L++LDN G+WN+R E  +  YLGQ+ Y  +++P  +   E + P+N + CG ++ M
Sbjct: 505 TAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of MC02g_new0003 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.1e-149
Identity = 260/558 (46.59%), Postives = 374/558 (67.03%), Query Frame = 0

Query: 4   LMFTVLLCLSAAAMSTVR-GEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINST 63
           L+ + LLC   A +S V    DP+  + + ++Y T SPLGVPQQ I +NGQFPGP +N+T
Sbjct: 7   LLASFLLCF--ALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNAT 66

Query: 64  SNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGS 123
           +N N+VVNVFN+LDEP L+ W GIQ R+NSWQDG+LGTNCPIPP  N+TY FQVKDQIGS
Sbjct: 67  TNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGS 126

Query: 124 FFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSG 183
           FFY PS    RA+GGFG + +N+R +IP+P+  P+ +   ++GDWYT+ H AL+  LDSG
Sbjct: 127 FFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSG 186

Query: 184 RSLARPDGVLINGK------NAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDH 243
           + L  PDGVLINGK      ++  DG D   F ++P KTY+ RV N+G+ TSLN R Q+H
Sbjct: 187 KELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNH 246

Query: 244 TMKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQE-PKDYYLIASTRFTKNV----LV 303
           ++ LVE EG +  Q ++   D+HVGQ +S LVT +Q+   DYY++AS RF        + 
Sbjct: 247 SLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVT 306

Query: 304 GKGIIRYTNGKGPASP--EIPEAPVGWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSI 363
           G  I+ Y+N KGP S    +P+  V   WS ++Q +T R N +AS ARPNPQGS+HYG I
Sbjct: 307 GVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQI 366

Query: 364 NITRTIKIINS--ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGP 423
           NIT T  I+ S   T ++G LR  +NG+S ++P TP++LA+   +    +K D    D P
Sbjct: 367 NITNTY-ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD--FPDRP 426

Query: 424 AEGANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKN 483
                 + +  +++NAT++ F+++VF+N++  +QS+H++GYSFF V ++ G W+ +K+ +
Sbjct: 427 FN--RPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGS 486

Query: 484 YNLLDAVSRDTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLR 543
           YN  DA+SR TI+V+P  W A+L++LDN G+WN+R E  +  YLG++ Y  + +P    +
Sbjct: 487 YNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGK 546

Query: 544 DEYNIPDNTLLCGLVKDM 545
            E + PDN L CG +K++
Sbjct: 547 TEMDPPDNVLYCGALKNL 556

BLAST of MC02g_new0003 vs. NCBI nr
Match: XP_022153749.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60
           MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of MC02g_new0003 vs. NCBI nr
Match: XP_022153757.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1113 bits (2878), Expect = 0.0
Identity = 533/552 (96.56%), Postives = 539/552 (97.64%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60
           M GL+FTVLLCLSAAAMSTVRGEDPYFFFTWN+TYGTI PLGV QQGILINGQFPGPNIN
Sbjct: 1   MGGLIFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTIXPLGVXQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSHS LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAXGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSXLKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQ HTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPXGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIXDKVFKYDTISDDGXAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPG+W PEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480

Query: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAA+LLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDM LPKPYTI
Sbjct: 541 VKDMALPKPYTI 552

BLAST of MC02g_new0003 vs. NCBI nr
Match: KAA0040123.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 504/553 (91.14%), Postives = 534/553 (96.56%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLMFT LLCLSAAAM +TV+GEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+V+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQ HTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPGRW+PEKR NYNLLDAVSR TIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0003 vs. NCBI nr
Match: KAA0040126.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 504/553 (91.14%), Postives = 533/553 (96.38%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           MA LMFT LLCLSAAAM +TVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+V+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQ HTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPGRW+PEKR NYNLLDAVSR TIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0003 vs. NCBI nr
Match: XP_022137060.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1070 bits (2766), Expect = 0.0
Identity = 503/551 (91.29%), Postives = 528/551 (95.83%), Query Frame = 0

Query: 3   GLMFTVLLCLSAAAM-STVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINS 62
           GLMFT+LLCL AAAM STVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNINS
Sbjct: 5   GLMFTMLLCLLAAAMMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64

Query: 63  TSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 122
           T+NNN+VVNVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQIG
Sbjct: 65  TTNNNLVVNVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDS 182
           S+FYYPSTAMHRAAG FGGLRVNSRLLIPVPYADPEDDYTVL+GDWY KSHS LKNFLDS
Sbjct: 125 SYFYYPSTAMHRAAGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYXKSHSTLKNFLDS 184

Query: 183 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLV 242
           GRSLARPDGVLINGK AKGDGTDEPLFTMKP KTYKYR+CN+GLKT+LN RFQ HTMKLV
Sbjct: 185 GRSLARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTTLNFRFQGHTMKLV 244

Query: 243 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 302
           EMEGSHVVQNDYQSLDIHVGQCFSVLV ANQEPKDYY++ASTRF K++LVGKGI+RYTNG
Sbjct: 245 EMEGSHVVQNDYQSLDIHVGQCFSVLVRANQEPKDYYMVASTRFIKSLLVGKGIVRYTNG 304

Query: 303 KGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 362
           KGPASPEIPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NSAT
Sbjct: 305 KGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSAT 364

Query: 363 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 422
           K DGKL+YAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD+GP E A TVT+APNVVN
Sbjct: 365 KTDGKLQYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDEGPTESATTVTIAPNVVN 424

Query: 423 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVF 482
           ATFRNFVEI+FENHEKSLQSWH +GYSFFAVAIEPGRWTPEKRKNYNLLDAVSR TIQVF
Sbjct: 425 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 484

Query: 483 PKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 542
           PKSWAAILLTLDN GMWNLRSELTENRYLGQQLY SV+SPA SLRDEYNIPDN LLCGLV
Sbjct: 485 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVISPAHSLRDEYNIPDNALLCGLV 544

Query: 543 KDMPLPKPYTI 552
           K MPLP PYTI
Sbjct: 545 KGMPLPXPYTI 555

BLAST of MC02g_new0003 vs. ExPASy TrEMBL
Match: A0A6J1DK02 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 SV=1)

HSP 1 Score: 1147 bits (2966), Expect = 0.0
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60
           MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of MC02g_new0003 vs. ExPASy TrEMBL
Match: A0A6J1DJT4 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021199 PE=3 SV=1)

HSP 1 Score: 1113 bits (2878), Expect = 0.0
Identity = 533/552 (96.56%), Postives = 539/552 (97.64%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60
           M GL+FTVLLCLSAAAMSTVRGEDPYFFFTWN+TYGTI PLGV QQGILINGQFPGPNIN
Sbjct: 1   MGGLIFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTIXPLGVXQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSHS LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAXGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSXLKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQ HTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPXGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIXDKVFKYDTISDDGXAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPG+W PEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480

Query: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAA+LLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDM LPKPYTI
Sbjct: 541 VKDMALPKPYTI 552

BLAST of MC02g_new0003 vs. ExPASy TrEMBL
Match: A0A5A7TAM1 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00980 PE=3 SV=1)

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 504/553 (91.14%), Postives = 534/553 (96.56%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           MAGLMFT LLCLSAAAM +TV+GEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+V+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQ HTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPGRW+PEKR NYNLLDAVSR TIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0003 vs. ExPASy TrEMBL
Match: A0A5A7TDG2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001010 PE=3 SV=1)

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 504/553 (91.14%), Postives = 533/553 (96.38%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNI 60
           MA LMFT LLCLSAAAM +TVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+V+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRAAGGFGGLR+NSRLLIPVPYADPEDDYTVL+GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQ HTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPGRW+PEKR NYNLLDAVSR TIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0003 vs. ExPASy TrEMBL
Match: A0A6J1C5L8 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111008621 PE=3 SV=1)

HSP 1 Score: 1070 bits (2766), Expect = 0.0
Identity = 503/551 (91.29%), Postives = 528/551 (95.83%), Query Frame = 0

Query: 3   GLMFTVLLCLSAAAM-STVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINS 62
           GLMFT+LLCL AAAM STVRGEDPYFFFTWN+TYGTISPLGVPQQGILINGQFPGPNINS
Sbjct: 5   GLMFTMLLCLLAAAMMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64

Query: 63  TSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 122
           T+NNN+VVNVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQIG
Sbjct: 65  TTNNNLVVNVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDS 182
           S+FYYPSTAMHRAAG FGGLRVNSRLLIPVPYADPEDDYTVL+GDWY KSHS LKNFLDS
Sbjct: 125 SYFYYPSTAMHRAAGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYXKSHSTLKNFLDS 184

Query: 183 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLV 242
           GRSLARPDGVLINGK AKGDGTDEPLFTMKP KTYKYR+CN+GLKT+LN RFQ HTMKLV
Sbjct: 185 GRSLARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTTLNFRFQGHTMKLV 244

Query: 243 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 302
           EMEGSHVVQNDYQSLDIHVGQCFSVLV ANQEPKDYY++ASTRF K++LVGKGI+RYTNG
Sbjct: 245 EMEGSHVVQNDYQSLDIHVGQCFSVLVRANQEPKDYYMVASTRFIKSLLVGKGIVRYTNG 304

Query: 303 KGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 362
           KGPASPEIPEAP+GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NSAT
Sbjct: 305 KGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSAT 364

Query: 363 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 422
           K DGKL+YAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD+GP E A TVT+APNVVN
Sbjct: 365 KTDGKLQYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDEGPTESATTVTIAPNVVN 424

Query: 423 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVF 482
           ATFRNFVEI+FENHEKSLQSWH +GYSFFAVAIEPGRWTPEKRKNYNLLDAVSR TIQVF
Sbjct: 425 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 484

Query: 483 PKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 542
           PKSWAAILLTLDN GMWNLRSELTENRYLGQQLY SV+SPA SLRDEYNIPDN LLCGLV
Sbjct: 485 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVISPAHSLRDEYNIPDNALLCGLV 544

Query: 543 KDMPLPKPYTI 552
           K MPLP PYTI
Sbjct: 545 KGMPLPXPYTI 555

BLAST of MC02g_new0003 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 915.2 bits (2364), Expect = 2.5e-266
Identity = 409/546 (74.91%), Postives = 473/546 (86.63%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSN 64
           +    L L+AAA   VR EDPYF   WN+TYGT+SPLGVPQQ ILINGQFPGPN+NSTSN
Sbjct: 6   LLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNSTSN 65

Query: 65  NNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
           NN+++NVFNNLDEPFL+ W+GIQHRKN WQDG  GT CPI PGTNYTYHFQ KDQIGS+F
Sbjct: 66  NNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYF 125

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRS 184
           YYPSTAMHR+AGGFGGLRVNSRLLIPVPYADPEDDYTVL+GDWYTKSH+ LK FLDSGR+
Sbjct: 126 YYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDSGRT 185

Query: 185 LARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVEME 244
           L RPDG+LINGK+ KGDG+D PLFT+KP KTY+ R+CN+GLKTSLN R Q+H +KLVEME
Sbjct: 186 LGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLVEME 245

Query: 245 GSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGP 304
           GSHV+QNDY SLD+HVGQC+  ++TANQE KDYY++AS+RF K+V+   G++RY  GKGP
Sbjct: 246 GSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGGKGP 305

Query: 305 ASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVD 364
           AS ++P  PVGWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  KVD
Sbjct: 306 ASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 365

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNATF 424
           GKLRYA+NGVSH DPETPLKLAEYFG+ADKVFKYD+I+D+   E   ++ + PNV+N T 
Sbjct: 366 GKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLNITH 425

Query: 425 RNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFPKS 484
           R F+E+VFENHEKS+QSWHL+GYSFFAVA+EPG WTPEKRKNYNLLDAVSR T+QV+PK 
Sbjct: 426 RTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 485

Query: 485 WAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 544
           WAAILLT DN GMWN+RSE +E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK  
Sbjct: 486 WAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGT 545

Query: 545 PLPKPY 551
           P P PY
Sbjct: 546 PKPNPY 551

BLAST of MC02g_new0003 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 909.1 bits (2348), Expect = 1.8e-264
Identity = 408/546 (74.73%), Postives = 469/546 (85.90%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSN 64
           +  V LC++ A +  V+ EDPYF   WN+TYGT SPLGVPQQ ILINGQFPGPNINSTSN
Sbjct: 7   LLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSN 66

Query: 65  NNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
           NN++VNVFNNLDEPFLI W+GIQHRKN WQDG  GT CPIPPG N+TYHFQ KDQIGS+F
Sbjct: 67  NNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIGSYF 126

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRS 184
           YYP+TAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYT+L+ DWYTKSH+ LK FLDSGR+
Sbjct: 127 YYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDSGRT 186

Query: 185 LARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVEME 244
           + RPDG+LINGK+ K DG+D+PLFT+KP KTY+ R+CN+GLK SLN R Q+H MKLVEME
Sbjct: 187 IGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVEME 246

Query: 245 GSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGP 304
           GSHV+QNDY SLD+HVGQCF V+VTA+QEPKDYY+IASTRF K  L   G++RY  GKGP
Sbjct: 247 GSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGKGP 306

Query: 305 ASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVD 364
           AS ++P APVGWAWSLNQ+R+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  KVD
Sbjct: 307 ASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVD 366

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNATF 424
           GKLRYA++GVSH DPETPLKLAEYFG+ADKVFKYDTISD+   +    + + PNV+N T 
Sbjct: 367 GKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNITH 426

Query: 425 RNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFPKS 484
           R F+E+VFENHE+S+QSWHL+GYSFFAVA+EPG WTPEKRKNYNLLDAVSR T+QV+PK 
Sbjct: 427 RTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKC 486

Query: 485 WAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 544
           WAAILLT DN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK  
Sbjct: 487 WAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGK 546

Query: 545 PLPKPY 551
           P   PY
Sbjct: 547 PKVNPY 552

BLAST of MC02g_new0003 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 882.1 bits (2278), Expect = 2.3e-256
Identity = 404/550 (73.45%), Postives = 471/550 (85.64%), Query Frame = 0

Query: 3   GLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINST 62
           G + TVL+CL A+ ++ V   DPYF++TWN+TYGT +PLG+PQQ ILINGQFPGPN+NST
Sbjct: 4   GRLLTVLVCL-ASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNST 63

Query: 63  SNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGS 122
           SNNN+V+NVFNNLDEPFL+ WSG+QHRKNSWQDG+ GT+CPIP GTN+TYHFQ KDQIGS
Sbjct: 64  SNNNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGS 123

Query: 123 FFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSG 182
           +FYYPSTA+HR AGGFGGLRVNSRLLIPVPYADPEDD T+L+ DWY KSH+ALKNFLDSG
Sbjct: 124 YFYYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSG 183

Query: 183 RSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVE 242
           R+L  PDGVLINGK+ K  G + PLFTMKP KTYKYR+CN+G K++LN R Q H MKLVE
Sbjct: 184 RTLGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVE 243

Query: 243 MEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGK 302
           MEGSHV+QNDY SLD+HVGQCF+VLVTA+Q  K+YY++ASTRF K  +   G++ Y    
Sbjct: 244 MEGSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSN 303

Query: 303 GPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATK 362
             AS +IP+APVGWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+ N+   
Sbjct: 304 VQASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNL 363

Query: 363 VDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNA 422
           V+GK+R+  NGVSH+D ETPLKLAEYFG+++KVFKY+ I D+ PA    T+TV PNV+N 
Sbjct: 364 VNGKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDE-PAAKITTLTVEPNVLNI 423

Query: 423 TFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFP 482
           TFR FVE+VFENHEKS+QS+HL+GYSFFAVA EPGRWTPEKR NYNLLDAVSR T+QV+P
Sbjct: 424 TFRTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYP 483

Query: 483 KSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVK 542
           KSW+AILLT DNAGMWN+RSE  E RYLGQQLY SVLSP +SLRDEYNIP NT LCG+VK
Sbjct: 484 KSWSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVK 543

Query: 543 DMPLPKPYTI 553
            +PLP PYTI
Sbjct: 544 GLPLPTPYTI 551

BLAST of MC02g_new0003 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 875.2 bits (2260), Expect = 2.8e-254
Identity = 401/551 (72.78%), Postives = 471/551 (85.48%), Query Frame = 0

Query: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60
           M G + TVL+CL  + ++ V   DPYFF TWN+TYGT SPLGVPQ+ ILINGQFPGPN+N
Sbjct: 1   MEGRLLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLN 60

Query: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNN+V+NVFN+LDEPFL+ WSGIQHRKN WQDG+ GT+CPIP G N+TYHFQ KDQI
Sbjct: 61  STSNNNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQI 120

Query: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180
           GS+FYYP+T++HR AGGFGGLRVNSRLLIPVPYADPEDDYTVL+GDWYT  H+ALKNFLD
Sbjct: 121 GSYFYYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240
           SGR+L  P+GVLINGK+ K  G +EPLFTMKP KTYKYR+CN+G K++LN R Q+H MKL
Sbjct: 181 SGRTLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHV+QNDY SLD+HVGQCFSVLVTANQ  KDYY++ASTRF K  L   G+IRY  
Sbjct: 241 VEMEGSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEG 300

Query: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
               AS E+P+APVGWAWSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+IK++NS 
Sbjct: 301 SNVQASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSK 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           + VDGK+R+  NGVSH+D ETPLKLAEYF +++KVFKY+ I D+ PA     +TV PNV+
Sbjct: 361 SVVDGKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDE-PAAKITALTVQPNVL 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480
           N TFR FVEI+FENHEK++QS+HL+GYSFFAVA EPGRWTPEKR+NYNLLDAVSR T+QV
Sbjct: 421 NITFRTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQV 480

Query: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           +PKSW+AILLT DNAGMWN+RSE  E +YLG+QLY SVLSP +SLRDEYNIP NT LCG+
Sbjct: 481 YPKSWSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGI 540

Query: 541 VKDMPLPKPYT 552
           VK +PLP  Y+
Sbjct: 541 VKGLPLPAHYS 549

BLAST of MC02g_new0003 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 642.5 bits (1656), Expect = 3.1e-184
Identity = 302/535 (56.45%), Postives = 389/535 (72.71%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNINSTSN 64
           M T LL L + A +    EDPY FF W++TYG ISPLGV QQGILING+FPGP+I S +N
Sbjct: 11  MITTLLFLISLAFA----EDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITN 70

Query: 65  NNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
           +N+++NVFN+LDEPFL+ W+GI++ KNS+QDG+ GT CPIPPG NYTY  QVKDQIGSF+
Sbjct: 71  DNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFY 130

Query: 125 YYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLDSGRS 184
           Y+PS   H+AAGGFGG+R++SR LIPVP+  P DDYT+LVGDWY  +H  LK  LD+G  
Sbjct: 131 YFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGK 190

Query: 185 LARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKLVEME 244
           L  PDG+LING+++           ++P KTY+ R+ N+GL+ SLN R Q+HTMKLVE+E
Sbjct: 191 LPLPDGILINGRSSGA------TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVE 250

Query: 245 GSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGP 304
           G + +QN + SLD+HVGQ +SVL+TA+Q  KDYY++ S+RFT  +L   G++ Y+N   P
Sbjct: 251 GRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAP 310

Query: 305 ASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVD 364
            S  IP+ P+  +WS NQ R  R NLTAS  RPNPQGSY YG INITRTI++ N+   ++
Sbjct: 311 VSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIE 370

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNATF 424
           GK RYA+N  S    +TPLKL +YF I D V+K  +ISD  P  GA  +    +V+ A F
Sbjct: 371 GKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ-PTNGA--IFPTTSVMQADF 430

Query: 425 RNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQVFPKS 484
           R FVE++FEN E  +QSWHL+GYSF+ V +E G+W+P  RK YNL DA+ R TIQV+P+S
Sbjct: 431 RAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRS 490

Query: 485 WAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           W AI + LDN GMWN+RSE+ E +YLGQQ Y  V + + SLRDEY IP N LLCG
Sbjct: 491 WTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531