MC02g_new0002 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g_new0002 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 3.4e-260
Identity = 406/550 (73.82%), Postives = 465/550 (84.55%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           G  +    L L+AAA   V  EDPYF   WNVTYGT +PLGV QQ ILINGQFPGPNINS
Sbjct: 3   GVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 62

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTNYTYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 122 SFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDS 181
           S+FYYP+T MHRA GG+GGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK FLD 
Sbjct: 123 SYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDG 182

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR++ RPDG++INGK+ KGDG+D PLFT+KP KTY+ R+CN+G+KTS+N R Q H MKLV
Sbjct: 183 GRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLV 242

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQCF  +VTANQEPKDYY++AS+RF K V+   G++RY  G
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGG 302

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
           KGPAS ++P  P GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  
Sbjct: 303 KGPASSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 362

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 421
           KVDGKLR+A+NGVSH +PETPLKLAEYFGI+DKVFKYDTI+DD   E    + + PNV+N
Sbjct: 363 KVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLN 422

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            T R FVE+VFENHEKS+QSWHL+GYSFF+VA+EPG W PEKRKNYNLLDAVSRHT+QV+
Sbjct: 423 ITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 482

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PK WAA+LLT DN GMWN+RSE TE RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLV
Sbjct: 483 PKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 542

Query: 542 KDMPLP-KPY 551
           K+ P P  PY
Sbjct: 543 KNTPKPVNPY 552

BLAST of MC02g_new0002 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.2e-238
Identity = 375/547 (68.56%), Postives = 448/547 (81.90%), Query Frame = 0

Query: 8   VLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSNNNI 67
           +LLCLS   ++    EDPY +F WNVTYGTI PLGV QQGILINGQFPGP IN TSNNNI
Sbjct: 11  LLLCLSVGVIA----EDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNI 70

Query: 68  VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYP 127
           V+NVFNNLDEPFL  W+G+QHRKNSWQDG  GT CPI PG N+TY FQVKDQIGS+ Y+P
Sbjct: 71  VVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFP 130

Query: 128 STAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDSGRSLAR 187
           +TA+HRA GG+G L V+SR LIPVP+ +P D+Y V  GDWY K H  LK  LD GR++ R
Sbjct: 131 TTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGR 190

Query: 188 PDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEMEGSH 247
           PDG++INGK+AK     EPLFTM+  KTY+YR CN+G+++S+NIRFQGH MKLVE+EGSH
Sbjct: 191 PDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSH 250

Query: 248 VVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGPASP 307
            VQN Y SLD+HVGQC SVLVTA+QEPKDYYL+ S+RF K  L    IIRY NGKGPASP
Sbjct: 251 TVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASP 310

Query: 308 EIPEAP----TGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKV 367
           E+P  P     G AWS+NQFR+FRWNLTASAARPNPQGSYHYG INITRTIKI NS ++V
Sbjct: 311 ELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQV 370

Query: 368 DGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNAT 427
            GKLRY +NG+SH + ETPLKL EYFG  +K FKYD ++D+ PA+  + +T+A NV NAT
Sbjct: 371 GGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPAD-PSKLTIATNVKNAT 430

Query: 428 FRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPK 487
           +RNFVEI+FENHEK+++++HL+GYSFFAVA+EPG+W+PEKRKNYNL+D +SR+ IQV+P 
Sbjct: 431 YRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPN 490

Query: 488 SWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKD 547
           SWAA++LT DNAGMWNLRSE+ E  YLG+QLYFSVLSP+RSLRDEYNIPDN  LCG+VK 
Sbjct: 491 SWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 550

Query: 548 MPLPKPY 551
           + +P PY
Sbjct: 551 LSMPAPY 552

BLAST of MC02g_new0002 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.5e-156
Identity = 269/562 (47.86%), Postives = 366/562 (65.12%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSN 64
           +F +LL +    +S     DPY F+ + V+Y T +PLGV QQ I ING+FPGP IN T+N
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
            N+V+NV N LDE  LLHW+GIQ R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFF
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 125 YYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDSGRS 184
           Y+PS    RA GGFG   VN R +IPVP++ P+ D TV  GDWY ++H+AL+  LD G+ 
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 185 LARPDGVLINGK-------NAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHT 244
           L  PDGVLINGK           DG D    T+ P KTY+ RV N+G+ TSLN R QGH 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 245 MKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQE-PKDYYLIASTRFTKNVL----VG 304
           + L E EGS+ VQ +Y SLDIHVGQ +S LVT +Q    DYY++AS R     +     G
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 305 KGIIRYTNGKGPASPEIPEAPT---GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSIN 364
            GI++YTN KG A  ++P  P       +S+NQ R+ RWN++AS ARPNPQGS+ YGSIN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 365 ITRTIKIIN-SATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD--DGP 424
           +T    + N     + GK R  +NG+S  +P TP++LA+   + D V+K D       GP
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLDFPKRPLTGP 422

Query: 425 AEGANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKN 484
           A+      VA +++N T+R F+E+V +N++  +QS+H++GY+FF V ++ G+W    R  
Sbjct: 423 AK------VATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGT 482

Query: 485 YNLLDAVSRHTIQVFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLR 544
           YN  D ++R TIQV+P +W+A+L++LDN G WNLR+E  ++ YLGQ+ Y  V++P  + +
Sbjct: 483 YNKWDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNK 542

Query: 545 DEYNIPDNTLLCGLVKDMPLPK 549
            E+  PDN L CG +  +  P+
Sbjct: 543 TEFGHPDNVLYCGALSKLQKPQ 557

BLAST of MC02g_new0002 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.8e-148
Identity = 248/539 (46.01%), Postives = 364/539 (67.53%), Query Frame = 0

Query: 24  DPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSNNNIVINVFNNLDEPFLLHW 83
           DPY  + + ++Y T +PLGV QQ I +NG+FPGP IN+T+N N+ +NV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 84  SGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFFYYPSTAMHRAGGGFGGLRV 143
            G+Q R+NSWQDG+LGTNCPIPP  N+TY FQ+KDQIGS+FY PS    RA GGFG L +
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 144 NSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDSGRSLARPDGVLINGK------N 203
           N+R L+P+P+ +P+ +   + GDWYT++H+AL+  LDSG+ L  PDGVLINGK      +
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 204 AKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEMEGSHVVQNDYQSLD 263
           +  DG +     + P KTY+ RV N+G+ TSLN R Q H + L+E EG +  Q ++   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 264 IHVGQCFSVLVTANQE-PKDYYLIASTRFTKNV----LVGKGIIRYTNGKGPASPEIPEA 323
           +HVGQ +S LVT +Q    DYY++AS RF        + G GI+ Y+N KGPAS  +P +
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 324 PT--GWAWS-LNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS--ATKVDGKL 383
            T     WS +NQ R  + N +AS ARPNPQGS+HYG INITRT  I+ S   TK++GKL
Sbjct: 325 ATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTY-ILRSLPPTKINGKL 384

Query: 384 RYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD--DGPAEGANTVTVAPNVVNATFR 443
           R  +NG+S ++P TP++LA+     D   K D + D  D P +      ++ +++NAT++
Sbjct: 385 RATLNGISFVNPSTPMRLAD-----DHKVKGDYMLDFPDRPLD-EKLPRLSSSIINATYK 444

Query: 444 NFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPKSW 503
            F++++F+N++  +QS+H++GY+F+ VA++ G W+ ++  +YN  DAV+R T++V+P +W
Sbjct: 445 GFIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAW 504

Query: 504 AAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 545
            AVL++LDN G+WN+R E  +  YLGQ+ Y  +++P  +   E + P+N + CG ++ M
Sbjct: 505 TAVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of MC02g_new0002 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.3e-147
Identity = 260/558 (46.59%), Postives = 370/558 (66.31%), Query Frame = 0

Query: 4   LMFTVLLCLSAAAMSTVR-GEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINST 63
           L+ + LLC   A +S V    DP+  + + V+Y T +PLGV QQ I +NGQFPGP +N+T
Sbjct: 7   LLASFLLCF--ALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNAT 66

Query: 64  SNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGS 123
           +N N+V+NVFN+LDEP LL W GIQ R+NSWQDG+LGTNCPIPP  N+TY FQVKDQIGS
Sbjct: 67  TNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGS 126

Query: 124 FFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDSG 183
           FFY PS    RA GGFG + +N+R +IP+P+  P+ +   + GDWYT+ H AL+  LDSG
Sbjct: 127 FFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSG 186

Query: 184 RSLARPDGVLINGK------NAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGH 243
           + L  PDGVLINGK      ++  DG D   F ++P KTY+ RV N+G+ TSLN R Q H
Sbjct: 187 KELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNH 246

Query: 244 TMKLVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQE-PKDYYLIASTRFTKNV----LV 303
           ++ LVE EG +  Q ++   D+HVGQ +S LVT +Q+   DYY++AS RF        + 
Sbjct: 247 SLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVT 306

Query: 304 GKGIIRYTNGKGPASPEIPEAPTGWA--WS-LNQFRTFRWNLTASAARPNPQGSYHYGSI 363
           G  I+ Y+N KGP S  +P   T  +  WS ++Q +T R N +AS ARPNPQGS+HYG I
Sbjct: 307 GVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQI 366

Query: 364 NITRTIKIINS--ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGP 423
           NIT T  I+ S   T ++G LR  +NG+S ++P TP++LA+   +    +K D    D P
Sbjct: 367 NITNTY-ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD--FPDRP 426

Query: 424 AEGANTVTVAPNVVNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKN 483
                 + +  +++NAT++ F+++VF+N++  +QS+H++GYSFF V ++ G W+ +K+ +
Sbjct: 427 FN--RPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGS 486

Query: 484 YNLLDAVSRHTIQVFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLR 543
           YN  DA+SR TI+V+P  W AVL++LDN G+WN+R E  +  YLG++ Y  + +P    +
Sbjct: 487 YNNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGK 546

Query: 544 DEYNIPDNTLLCGLVKDM 545
            E + PDN L CG +K++
Sbjct: 547 TEMDPPDNVLYCGALKNL 556

BLAST of MC02g_new0002 vs. NCBI nr
Match: XP_022153757.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 543/552 (98.37%), Postives = 544/552 (98.55%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           MGGL+FTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTI PLGV QQGILINGQFPGPNIN
Sbjct: 1   MGGLIFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTIXPLGVXQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHS LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAXGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSXLKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPXGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIXDKVFKYDTISDDGXAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDM LPKPYTI
Sbjct: 541 VKDMALPKPYTI 552

BLAST of MC02g_new0002 vs. NCBI nr
Match: XP_022153749.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 538/552 (97.46%), Postives = 544/552 (98.55%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           M GLMFTVLLCLSAAAMSTVRGEDPYFFFTWN+TYGTI+PLGV QQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSHSALKNFLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQ HTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPG+W PEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAA+LLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of MC02g_new0002 vs. NCBI nr
Match: KAA0040123.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 502/553 (90.78%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M GLMFT LLCLSAAAM +TV+GEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRA GGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0002 vs. NCBI nr
Match: KAA0040126.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 502/553 (90.78%), Postives = 530/553 (95.84%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M  LMFT LLCLSAAAM +TVRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRA GGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0002 vs. NCBI nr
Match: XP_022137060.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 500/551 (90.74%), Postives = 525/551 (95.28%), Query Frame = 0

Query: 3   GLMFTVLLCLSAAAM-STVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 62
           GLMFT+LLCL AAAM STVRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNINS
Sbjct: 5   GLMFTMLLCLLAAAMMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64

Query: 63  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 122
           T+NNN+V+NVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQIG
Sbjct: 65  TTNNNLVVNVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDS 182
           S+FYYPSTAMHRA G FGGLRVNSRLLIPVPYADPEDDYTVL GDWY KSHS LKNFLDS
Sbjct: 125 SYFYYPSTAMHRAAGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYXKSHSTLKNFLDS 184

Query: 183 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 242
           GRSLARPDGVLINGK AKGDGTDEPLFTMKP KTYKYR+CN+GLKT+LN RFQGHTMKLV
Sbjct: 185 GRSLARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTTLNFRFQGHTMKLV 244

Query: 243 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 302
           EMEGSHVVQNDYQSLDIHVGQCFSVLV ANQEPKDYY++ASTRF K++LVGKGI+RYTNG
Sbjct: 245 EMEGSHVVQNDYQSLDIHVGQCFSVLVRANQEPKDYYMVASTRFIKSLLVGKGIVRYTNG 304

Query: 303 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 362
           KGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NSAT
Sbjct: 305 KGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSAT 364

Query: 363 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 422
           K DGKL+YAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD+GP E A TVT+APNVVN
Sbjct: 365 KTDGKLQYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDEGPTESATTVTIAPNVVN 424

Query: 423 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 482
           ATFRNFVEI+FENHEKSLQSWH +GYSFFAVAIEPG+W PEKRKNYNLLDAVSRHTIQVF
Sbjct: 425 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 484

Query: 483 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 542
           PKSWAA+LLTLDN GMWNLRSELTENRYLGQQLY SV+SPA SLRDEYNIPDN LLCGLV
Sbjct: 485 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVISPAHSLRDEYNIPDNALLCGLV 544

Query: 543 KDMPLPKPYTI 552
           K MPLP PYTI
Sbjct: 545 KGMPLPXPYTI 555

BLAST of MC02g_new0002 vs. ExPASy TrEMBL
Match: A0A6J1DJT4 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021199 PE=3 SV=1)

HSP 1 Score: 1128 bits (2917), Expect = 0.0
Identity = 543/552 (98.37%), Postives = 544/552 (98.55%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           MGGL+FTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTI PLGV QQGILINGQFPGPNIN
Sbjct: 1   MGGLIFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTIXPLGVXQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHS LKNFLD
Sbjct: 121 GSFFYYPSTAMHRAXGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSXLKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPXGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGI DKVFKYDTISDDG AEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIXDKVFKYDTISDDGXAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDM LPKPYTI
Sbjct: 541 VKDMALPKPYTI 552

BLAST of MC02g_new0002 vs. ExPASy TrEMBL
Match: A0A6J1DK02 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111021193 PE=3 SV=1)

HSP 1 Score: 1126 bits (2913), Expect = 0.0
Identity = 538/552 (97.46%), Postives = 544/552 (98.55%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           M GLMFTVLLCLSAAAMSTVRGEDPYFFFTWN+TYGTI+PLGV QQGILINGQFPGPNIN
Sbjct: 1   MAGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNITYGTISPLGVPQQGILINGQFPGPNIN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNNIV+NVFNNLDEPFL+HWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI
Sbjct: 61  STSNNNIVVNVFNNLDEPFLIHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120

Query: 121 GSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLD 180
           GSFFYYPSTAMHRA GGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSHSALKNFLD
Sbjct: 121 GSFFYYPSTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTVLVGDWYTKSHSALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQ HTMKL
Sbjct: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQDHTMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN
Sbjct: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
           GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA
Sbjct: 301 GKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV
Sbjct: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPG+W PEKRKNYNLLDAVSR TIQV
Sbjct: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRDTIQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           FPKSWAA+LLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL
Sbjct: 481 FPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540

Query: 541 VKDMPLPKPYTI 552
           VKDMPLPKPYTI
Sbjct: 541 VKDMPLPKPYTI 552

BLAST of MC02g_new0002 vs. ExPASy TrEMBL
Match: A0A5A7TAM1 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00980 PE=3 SV=1)

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 502/553 (90.78%), Postives = 531/553 (96.02%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M GLMFT LLCLSAAAM +TV+GEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MAGLMFTALLCLSAAAMMATVQGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRA GGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0002 vs. ExPASy TrEMBL
Match: A0A5A7TDG2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001010 PE=3 SV=1)

HSP 1 Score: 1067 bits (2760), Expect = 0.0
Identity = 502/553 (90.78%), Postives = 530/553 (95.84%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAM-STVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNI 60
           M  LMFT LLCLSAAAM +TVRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQ 120
           NSTSNNN+VINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPI PGTN+TYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFL 180
           IGSFFYYPSTAMHRA GGFGGLR+NSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK +L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMK 240
           DSGRS+ARPDGVLINGK AKGDGTDEPLFTMKP KTYKYRVCN+GLK+SLN RFQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYT 300
           LVEMEGSH VQNDYQSLD+HVGQCFSVLVTANQE KDYY++ASTRF KNVLVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINS 360
           +GKGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNV 420
           A+KVDGKLRYAINGVSH+DPETPLKLAEYFGIADKVFKYDTISD+G AEGA TVTVAPNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EI+FENHEKSLQSWHL+GYSFFAVAIEPG+W+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAA+LLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 552
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of MC02g_new0002 vs. ExPASy TrEMBL
Match: A0A6J1C5L8 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111008621 PE=3 SV=1)

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 500/551 (90.74%), Postives = 525/551 (95.28%), Query Frame = 0

Query: 3   GLMFTVLLCLSAAAM-STVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 62
           GLMFT+LLCL AAAM STVRGEDPYFFFTWNVTYGTI+PLGV QQGILINGQFPGPNINS
Sbjct: 5   GLMFTMLLCLLAAAMMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 64

Query: 63  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 122
           T+NNN+V+NVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTN+TYHFQVKDQIG
Sbjct: 65  TTNNNLVVNVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDS 182
           S+FYYPSTAMHRA G FGGLRVNSRLLIPVPYADPEDDYTVL GDWY KSHS LKNFLDS
Sbjct: 125 SYFYYPSTAMHRAAGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYXKSHSTLKNFLDS 184

Query: 183 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 242
           GRSLARPDGVLINGK AKGDGTDEPLFTMKP KTYKYR+CN+GLKT+LN RFQGHTMKLV
Sbjct: 185 GRSLARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRICNVGLKTTLNFRFQGHTMKLV 244

Query: 243 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 302
           EMEGSHVVQNDYQSLDIHVGQCFSVLV ANQEPKDYY++ASTRF K++LVGKGI+RYTNG
Sbjct: 245 EMEGSHVVQNDYQSLDIHVGQCFSVLVRANQEPKDYYMVASTRFIKSLLVGKGIVRYTNG 304

Query: 303 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 362
           KGPASPEIPEAP GWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIK++NSAT
Sbjct: 305 KGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNSAT 364

Query: 363 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 422
           K DGKL+YAINGVSHIDPETPLKLAEYFGIADKVFKYDTISD+GP E A TVT+APNVVN
Sbjct: 365 KTDGKLQYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDEGPTESATTVTIAPNVVN 424

Query: 423 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 482
           ATFRNFVEI+FENHEKSLQSWH +GYSFFAVAIEPG+W PEKRKNYNLLDAVSRHTIQVF
Sbjct: 425 ATFRNFVEIIFENHEKSLQSWHFDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 484

Query: 483 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 542
           PKSWAA+LLTLDN GMWNLRSELTENRYLGQQLY SV+SPA SLRDEYNIPDN LLCGLV
Sbjct: 485 PKSWAAILLTLDNVGMWNLRSELTENRYLGQQLYISVISPAHSLRDEYNIPDNALLCGLV 544

Query: 543 KDMPLPKPYTI 552
           K MPLP PYTI
Sbjct: 545 KGMPLPXPYTI 555

BLAST of MC02g_new0002 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 907.9 bits (2345), Expect = 4.0e-264
Identity = 408/549 (74.32%), Postives = 469/549 (85.43%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           G  +    L L+AAA   VR EDPYF   WNVTYGT++PLGV QQ ILINGQFPGPN+NS
Sbjct: 3   GVKLLAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNS 62

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN++INVFNNLDEPFLL W+GIQHRKN WQDG  GT CPI PGTNYTYHFQ KDQIG
Sbjct: 63  TSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIG 122

Query: 122 SFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDS 181
           S+FYYPSTAMHR+ GGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYTKSH+ LK FLDS
Sbjct: 123 SYFYYPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDS 182

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR+L RPDG+LINGK+ KGDG+D PLFT+KP KTY+ R+CN+GLKTSLN R Q H +KLV
Sbjct: 183 GRTLGRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLV 242

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQC+  ++TANQE KDYY++AS+RF K+V+   G++RY  G
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGG 302

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
           KGPAS ++P  P GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  
Sbjct: 303 KGPASSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 362

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 421
           KVDGKLRYA+NGVSH DPETPLKLAEYFG+ADKVFKYD+I+D+   E   ++ + PNV+N
Sbjct: 363 KVDGKLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLN 422

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            T R F+E+VFENHEKS+QSWHL+GYSFFAVA+EPG W PEKRKNYNLLDAVSRHT+QV+
Sbjct: 423 ITHRTFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 482

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PK WAA+LLT DN GMWN+RSE +E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLV
Sbjct: 483 PKCWAAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 542

Query: 542 KDMPLPKPY 551
           K  P P PY
Sbjct: 543 KGTPKPNPY 551

BLAST of MC02g_new0002 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 899.4 bits (2323), Expect = 1.4e-261
Identity = 403/549 (73.41%), Postives = 465/549 (84.70%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           G  +  V LC++ A +  V+ EDPYF   WNVTYGT +PLGV QQ ILINGQFPGPNINS
Sbjct: 4   GVKLLAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINS 63

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN+++NVFNNLDEPFL+ W+GIQHRKN WQDG  GT CPIPPG N+TYHFQ KDQIG
Sbjct: 64  TSNNNVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIG 123

Query: 122 SFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDS 181
           S+FYYP+TAMHRA GGFGGLRVNSRLLIPVPYADPEDDYT+L  DWYTKSH+ LK FLDS
Sbjct: 124 SYFYYPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDS 183

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR++ RPDG+LINGK+ K DG+D+PLFT+KP KTY+ R+CN+GLK SLN R Q H MKLV
Sbjct: 184 GRTIGRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLV 243

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQCF V+VTA+QEPKDYY+IASTRF K  L   G++RY  G
Sbjct: 244 EMEGSHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGG 303

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
           KGPAS ++P AP GWAWSLNQ+R+FRWNLTASAARPNPQGSYHYG INITRTIK++N+  
Sbjct: 304 KGPASSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQG 363

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 421
           KVDGKLRYA++GVSH DPETPLKLAEYFG+ADKVFKYDTISD+   +    + + PNV+N
Sbjct: 364 KVDGKLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLN 423

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            T R F+E+VFENHE+S+QSWHL+GYSFFAVA+EPG W PEKRKNYNLLDAVSRHT+QV+
Sbjct: 424 ITHRTFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVY 483

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PK WAA+LLT DN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLV
Sbjct: 484 PKCWAAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLV 543

Query: 542 KDMPLPKPY 551
           K  P   PY
Sbjct: 544 KGKPKVNPY 552

BLAST of MC02g_new0002 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 879.4 bits (2271), Expect = 1.5e-255
Identity = 404/551 (73.32%), Postives = 468/551 (84.94%), Query Frame = 0

Query: 2   GGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINS 61
           GG + TVL+CL A+ ++ V   DPYF++TWNVTYGT  PLG+ QQ ILINGQFPGPN+NS
Sbjct: 3   GGRLLTVLVCL-ASTVALVSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNS 62

Query: 62  TSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIG 121
           TSNNN+VINVFNNLDEPFLL WSG+QHRKNSWQDG+ GT+CPIP GTN+TYHFQ KDQIG
Sbjct: 63  TSNNNVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIG 122

Query: 122 SFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDS 181
           S+FYYPSTA+HR  GGFGGLRVNSRLLIPVPYADPEDD T+L  DWY KSH+ALKNFLDS
Sbjct: 123 SYFYYPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDS 182

Query: 182 GRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLV 241
           GR+L  PDGVLINGK+ K  G + PLFTMKP KTYKYR+CN+G K++LN R QGH MKLV
Sbjct: 183 GRTLGSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLV 242

Query: 242 EMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNG 301
           EMEGSHV+QNDY SLD+HVGQCF+VLVTA+Q  K+YY++ASTRF K  +   G++ Y   
Sbjct: 243 EMEGSHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGS 302

Query: 302 KGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSAT 361
              AS +IP+AP GWAWSLNQFR+FRWNLTASAARPNPQGSYHYG INITRTIK+ N+  
Sbjct: 303 NVQASSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKN 362

Query: 362 KVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVN 421
            V+GK+R+  NGVSH+D ETPLKLAEYFG+++KVFKY+ I D+ PA    T+TV PNV+N
Sbjct: 363 LVNGKVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDE-PAAKITTLTVEPNVLN 422

Query: 422 ATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVF 481
            TFR FVE+VFENHEKS+QS+HL+GYSFFAVA EPG+W PEKR NYNLLDAVSRHT+QV+
Sbjct: 423 ITFRTFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVY 482

Query: 482 PKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLV 541
           PKSW+A+LLT DNAGMWN+RSE  E RYLGQQLY SVLSP +SLRDEYNIP NT LCG+V
Sbjct: 483 PKSWSAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIV 542

Query: 542 KDMPLPKPYTI 553
           K +PLP PYTI
Sbjct: 543 KGLPLPTPYTI 551

BLAST of MC02g_new0002 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 867.5 bits (2240), Expect = 5.9e-252
Identity = 398/551 (72.23%), Postives = 466/551 (84.57%), Query Frame = 0

Query: 1   MGGLMFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNIN 60
           M G + TVL+CL  + ++ V   DPYFF TWNVTYGT +PLGV Q+ ILINGQFPGPN+N
Sbjct: 1   MEGRLLTVLVCL-VSTVAIVNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLN 60

Query: 61  STSNNNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQI 120
           STSNNN+VINVFN+LDEPFLL WSGIQHRKN WQDG+ GT+CPIP G N+TYHFQ KDQI
Sbjct: 61  STSNNNVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQI 120

Query: 121 GSFFYYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLD 180
           GS+FYYP+T++HR  GGFGGLRVNSRLLIPVPYADPEDDYTVL GDWYT  H+ALKNFLD
Sbjct: 121 GSYFYYPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLD 180

Query: 181 SGRSLARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKL 240
           SGR+L  P+GVLINGK+ K  G +EPLFTMKP KTYKYR+CN+G K++LN R Q H MKL
Sbjct: 181 SGRTLGLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKL 240

Query: 241 VEMEGSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTN 300
           VEMEGSHV+QNDY SLD+HVGQCFSVLVTANQ  KDYY++ASTRF K  L   G+IRY  
Sbjct: 241 VEMEGSHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEG 300

Query: 301 GKGPASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSA 360
               AS E+P+AP GWAWSLNQFR+FRWNLT++AARPNPQGSYHYG INITR+IK++NS 
Sbjct: 301 SNVQASTELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSK 360

Query: 361 TKVDGKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVV 420
           + VDGK+R+  NGVSH+D ETPLKLAEYF +++KVFKY+ I D+ PA     +TV PNV+
Sbjct: 361 SVVDGKVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDE-PAAKITALTVQPNVL 420

Query: 421 NATFRNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQV 480
           N TFR FVEI+FENHEK++QS+HL+GYSFFAVA EPG+W PEKR+NYNLLDAVSRHT+QV
Sbjct: 421 NITFRTFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQV 480

Query: 481 FPKSWAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGL 540
           +PKSW+A+LLT DNAGMWN+RSE  E +YLG+QLY SVLSP +SLRDEYNIP NT LCG+
Sbjct: 481 YPKSWSAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGI 540

Query: 541 VKDMPLPKPYT 552
           VK +PLP  Y+
Sbjct: 541 VKGLPLPAHYS 549

BLAST of MC02g_new0002 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 637.9 bits (1644), Expect = 7.6e-183
Identity = 302/535 (56.45%), Postives = 385/535 (71.96%), Query Frame = 0

Query: 5   MFTVLLCLSAAAMSTVRGEDPYFFFTWNVTYGTITPLGVTQQGILINGQFPGPNINSTSN 64
           M T LL L + A +    EDPY FF W+VTYG I+PLGV QQGILING+FPGP+I S +N
Sbjct: 11  MITTLLFLISLAFA----EDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITN 70

Query: 65  NNIVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNYTYHFQVKDQIGSFF 124
           +N++INVFN+LDEPFLL W+GI++ KNS+QDG+ GT CPIPPG NYTY  QVKDQIGSF+
Sbjct: 71  DNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFY 130

Query: 125 YYPSTAMHRAGGGFGGLRVNSRLLIPVPYADPEDDYTVLAGDWYTKSHSALKNFLDSGRS 184
           Y+PS   H+A GGFGG+R++SR LIPVP+  P DDYT+L GDWY  +H  LK  LD+G  
Sbjct: 131 YFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGK 190

Query: 185 LARPDGVLINGKNAKGDGTDEPLFTMKPEKTYKYRVCNIGLKTSLNIRFQGHTMKLVEME 244
           L  PDG+LING+++           ++P KTY+ R+ N+GL+ SLN R Q HTMKLVE+E
Sbjct: 191 LPLPDGILINGRSSGA------TLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVE 250

Query: 245 GSHVVQNDYQSLDIHVGQCFSVLVTANQEPKDYYLIASTRFTKNVLVGKGIIRYTNGKGP 304
           G + +QN + SLD+HVGQ +SVL+TA+Q  KDYY++ S+RFT  +L   G++ Y+N   P
Sbjct: 251 GRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAP 310

Query: 305 ASPEIPEAPTGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIINSATKVD 364
            S  IP+ P   +WS NQ R  R NLTAS  RPNPQGSY YG INITRTI++ N+   ++
Sbjct: 311 VSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIE 370

Query: 365 GKLRYAINGVSHIDPETPLKLAEYFGIADKVFKYDTISDDGPAEGANTVTVAPNVVNATF 424
           GK RYA+N  S    +TPLKL +YF I D V+K  +ISD  P  GA  +    +V+ A F
Sbjct: 371 GKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ-PTNGA--IFPTTSVMQADF 430

Query: 425 RNFVEIVFENHEKSLQSWHLNGYSFFAVAIEPGKWAPEKRKNYNLLDAVSRHTIQVFPKS 484
           R FVE++FEN E  +QSWHL+GYSF+ V +E GKW+P  RK YNL DA+ R TIQV+P+S
Sbjct: 431 RAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRS 490

Query: 485 WAAVLLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           W A+ + LDN GMWN+RSE+ E +YLGQQ Y  V + + SLRDEY IP N LLCG
Sbjct: 491 WTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531