MC02g1309 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g1309 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 2.9e-59
Identity = 254/963 (26.38%), Postives = 451/963 (46.83%), Query Frame = 0

Query: 27  YNRNIKDLEKQLENLKTTREKV-QILVNGRSNSYEIYDEVSTWL-LAEVNVQLPNMPQQN 86
           +  N + L + LE LK  + KV + L         +  ++  WL   E NV L  +  + 
Sbjct: 2   FRSNARALNRALERLKNVQTKVNEALKRSGIQEKSLERKLRIWLRKVEENVPLGELILEK 61

Query: 87  KGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILELVEKGKK-FDKVGYPVPLPHTKSPT 146
           +          SC  +     LS K  + ++ +  L E+G+    K+             
Sbjct: 62  RS---------SCAIW-----LSDKDVEILEKVKRLEEQGQDLIKKISVNKSSREIVERV 121

Query: 147 LPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGGVGKTKWLDEVKKLVLE---NKLF 206
           L   +   ++   + +K+KD L   NV K+GV+GMGGVGKT  +  +   +L+    + F
Sbjct: 122 LGPSFHPQKTALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQF 181

Query: 207 DRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILILLDD 266
             VI VT+ +   +  +Q  I  +L  G     E        +  +L++++ N L++LDD
Sbjct: 182 ALVIWVTVSKDFDLKRVQMDIAKRL--GKRFTREQMNQLGLTICERLIDLK-NFLLILDD 241

Query: 267 LWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESWKFF 326
           +W+  DL  ++GIP   E SK+  K+++TSR R  +   M T +  KV  L E+E+W+ F
Sbjct: 242 VWHPIDLD-QLGIPLALERSKDS-KVVLTSR-RLEVCQQMMTNENIKVACLQEKEAWELF 301

Query: 327 MTIVGEF-DKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK-ELRIWKNALEKLEKRVSV 386
              VGE  + + ++ +AK+V  +C GLP+A+  I + L+GK ++ +WK+ L  L++  S 
Sbjct: 302 CHNVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR--SA 361

Query: 387 KIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGLLNEVD 446
                 ++++  LKLSYD+++D   +  FL C++FP+D+ I   +L MY +  GLL+   
Sbjct: 362 PSIDTEEKIFGTLKLSYDFLQD-NMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQH 421

Query: 447 NWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASN--ENNMSSLSYG 506
           ++E   N  + LV+ LK S LL + +S D  V+MHDVVRD A +  S+  E   S +  G
Sbjct: 422 HYEDMMNEGVTLVERLKDSCLLEDGDSCDT-VKMHDVVRDFAIWFMSSQGEGFHSLVMAG 481

Query: 507 CGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQAIQIADTF 566
            G  E+ +     S   + +       LP N+    ++ L+L +Q   G     ++ + F
Sbjct: 482 RGLIEFPQDKFVSSVQRVSLMANKLERLPNNV-IEGVETLVLLLQ---GNSHVKEVPNGF 541

Query: 567 FEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYC-ECDDILTIFQLKTLEILRIN 626
            +    LR+LD++ +R  +   S +N L +L++L +  C +  ++ ++  L  L+ L ++
Sbjct: 542 LQAFPNLRILDLSGVRIRTLPDSFSN-LHSLRSLVLRNCKKLRNLPSLESLVKLQFLDLH 601

Query: 627 GSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGGEEVSY 686
            S I+ELP  ++ L+ L+ + VSN   L+ IPA  I  ++ LE L +  S    G +   
Sbjct: 602 ESAIRELPRGLEALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEE 661

Query: 687 KNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLERVEKLEEYCICVGDNEDFEIC 746
           +       +++ L HL  L+  +L++ +     D+L+ +R+ K +               
Sbjct: 662 REGQATLDEVTCLPHLQFLAIKLLDVLSFSYEFDSLT-KRLTKFQ--------------- 721

Query: 747 DEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRVKD------SVGF-INHLFKPNGNYC- 806
                 L   I+S      G      EGC  L + D      S+G+ + H+   + NYC 
Sbjct: 722 -----FLFSPIRSVSPPGTG------EGC--LAISDVNVSNASIGWLLQHVTSLDLNYCE 781

Query: 807 --YPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIYLFTTHFR 866
                 +NL  +  S         V  KA  + +F        C  ++ ++  L      
Sbjct: 782 GLNGMFENLVTKSKSSF-------VAMKALSIHYFPSLSLASGCESQLDLFPNLEELSLD 841

Query: 867 N----SITLLR-----KKQKLQTIRIQYCNKLKHAFPSNVVT-SLVFLNILEILHCALLE 926
           N    SI  L      + QKL+ +++  C +LK  F   ++  +L  L  ++++ C  LE
Sbjct: 842 NVNLESIGELNGFLGMRLQKLKLLQVSGCRQLKRLFSDQILAGTLPNLQEIKVVSCLRLE 895

Query: 927 GIFEVDML----TDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPHL 955
            +F    +      E+ +  L  + L  LP+L+ + N    + +   +L  +EV  C  L
Sbjct: 902 ELFNFSSVPVDFCAESLLPKLTVIKLKYLPQLRSLCN----DRVVLESLEHLEVESCESL 895

BLAST of MC02g1309 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.5e-55
Identity = 235/879 (26.73%), Postives = 420/879 (47.78%), Query Frame = 0

Query: 103 RHKLSRKSKKRVKDILELVEKGKKFDKV----GYPVPLPHTKSPTLPTDYQILESRTLLT 162
           R ++SRK  K + ++  L + G +F  +      P  + H   P +   +Q + S  L  
Sbjct: 97  RPRMSRKLVKILDEVKMLEKDGIEFVDMLSVESTPERVEHV--PGVSVVHQTMASNML-- 156

Query: 163 EKIKDALADPNVNKVGVYGMGGVGKTKWLDEVKKLVLE---NKLFDRVIDVTIGQSNGVV 222
            KI+D L      K+GV+GMGGVGKT  +  +   + E    + F  VI V + +     
Sbjct: 157 AKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTLNNKLREEGATQPFGLVIFVIVSKEFDPR 216

Query: 223 EIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPC 282
           E+Q QI  +L++   M+ E++E  A  +   L++ R+  L++LDD+W   DL   +GIP 
Sbjct: 217 EVQKQIAERLDIDTQME-ESEEKLARRIYVGLMKERK-FLLILDDVWKPIDLDL-LGIP- 276

Query: 283 RSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESWKFFMTIVGEFDK-NRIEH 342
           R+E +K G K+++TSR   +   +M T+   +V+ L EE++W+ F    G+  + + +  
Sbjct: 277 RTEENK-GSKVILTSRFLEV-CRSMKTDLDVRVDCLLEEDAWELFCKNAGDVVRSDHVRK 336

Query: 343 MAKNVVKKCGGLPIALKIIAKALKGKE-LRIWKNALEKLEKRVSVKIKGVSDQLYSCLKL 402
           +AK V ++CGGLP+A+  +  A++GK+ +++W + L KL K V   IK + ++++  LKL
Sbjct: 337 IAKAVSQECGGLPLAIITVGTAMRGKKNVKLWNHVLSKLSKSVP-WIKSIEEKIFQPLKL 396

Query: 403 SYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDH 462
           SYD++ED + +  FLLC++FP+D+ I   ++  Y M  G + E+ + E + N  I  V+ 
Sbjct: 397 SYDFLED-KAKFCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITTVES 456

Query: 463 LKSSSLLLESNSGDNYVQMHDVVRDVAKYI--ASNENNMSSLSYGCGQSEWREKDRYGSY 522
           LK   LL + +  D  V+MHDVVRD A +I  +S +++ S +  G G  + R+     S 
Sbjct: 457 LKDYCLLEDGDRRDT-VKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLAPSL 516

Query: 523 NAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKM 582
             + +      +LP  +E   ++  +L +Q   G     ++   F +    LR+L+++  
Sbjct: 517 RRVSLMNNKLESLPDLVEEFCVKTSVLLLQ---GNFLLKEVPIGFLQAFPTLRILNLSGT 576

Query: 583 RFESSRTSLTNSLKNLQTLCMSYC-ECDDILTIFQLKTLEILRINGSTIQELPATMDDLT 642
           R +S  +     L +L +L +  C +   + ++  L  LE+L + G+ I E P  +++L 
Sbjct: 577 RIKSFPSCSLLRLFSLHSLFLRDCFKLVKLPSLETLAKLELLDLCGTHILEFPRGLEELK 636

Query: 643 QLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGGEEVSYKNELIK-NVKLSXLN 702
           + + LD+S    LE IPA ++S ++ LE L +  S  R     S + E  K    +  + 
Sbjct: 637 RFRHLDLSRTLHLESIPARVVSRLSSLETLDMTSSHYRW----SVQGETQKGQATVEEIG 696

Query: 703 HLTHLSNLMLEIPNVEILSDNLSLERVEKLEEYCICVGDNEDFEICDEYARSLVVNIKSQ 762
            L  L  L + + +   L  N     +++L+++ + VG         +  R  + ++   
Sbjct: 697 CLQRLQVLSIRLHSSPFLL-NKRNTWIKRLKKFQLVVGSRYILRTRHDKRRLTISHLNVS 756

Query: 763 IVSNGGVL----EILLEGCEQLR------VKDSVGFINHLFKPNGNYCYPRMKNLHIE-- 822
            VS G +L     + L  C+ +       V D+ GF N             +K+L IE  
Sbjct: 757 QVSIGWLLAYTTSLALNHCQGIEAMMKKLVSDNKGFKN-------------LKSLTIENV 816

Query: 823 --GNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIYLFTTHFRNSITLLRKKQ 882
               +     +      ++  +     N       +++++      T       L  K +
Sbjct: 817 IINTNSWVEMVSTNTSKQSSDILDLLPN------LEELHLRRVDLETFSELQTHLGLKLE 876

Query: 883 KLQTIRIQYCNKLKHAFPSNVVTSLVFLNILEILHCALLEGIFEVDMLTDEAKVVPLKNL 942
            L+ I I  C KL+         ++  L  +EI +C  L+ + E  +L  +  V  L+ L
Sbjct: 877 TLKIIEITMCRKLRTLLDKRNFLTIPNLEEIEISYCDSLQNLHEA-LLYHQPFVPNLRVL 930

Query: 943 ILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPHLKTI 955
            L +LP L  + N        +  L QVEV  C  L  +
Sbjct: 937 KLRNLPNLVSICNWGE----VWECLEQVEVIHCNQLNCL 930

BLAST of MC02g1309 vs. ExPASy Swiss-Prot
Match: Q42484 (Disease resistance protein RPS2 OS=Arabidopsis thaliana OX=3702 GN=RPS2 PE=1 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 9.4e-50
Identity = 198/693 (28.57%), Postives = 331/693 (47.76%), Query Frame = 0

Query: 31  IKDLEKQLENLKTTREKVQILV-----NGRSNSYEIYDEVSTWLLAEVNVQLPNMPQQNK 90
           I DLE  + +LK  R+ + + +      GRS S    + +S   + E    L  +  + +
Sbjct: 35  ITDLETAIGDLKAIRDDLTLRIQQDGLEGRSCSNRAREWLSAVQVTETKTALLLVRFRRR 94

Query: 91  GVSNR-EASYPSCLNFFKRHKLSRKSKKRVKDILELVEKGKKFDKVGYPVPLPHTKSPTL 150
               R    Y SC      +KL +K    +K I EL E+ +     G  + +   + P  
Sbjct: 95  EQRTRMRRRYLSCFG-CADYKLCKKVSAILKSIGELRERSEAIKTDGGSIQVTCREIPI- 154

Query: 151 PTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGGVGKTKWLDEV-KKLVLENKLFDRV 210
                ++ + T++ + ++    +     +GVYG GGVGKT  +  +  +L+ +   +D +
Sbjct: 155 ---KSVVGNTTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVL 214

Query: 211 IDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILILLDDLWN 270
           I V + +  G   IQ  +G +L +  + K ET E RA  +   L + R   L+LLDD+W 
Sbjct: 215 IWVQMSREFGECTIQQAVGARLGLSWDEK-ETGENRALKIYRALRQKR--FLLLLDDVWE 274

Query: 271 EYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESWKFFMTI 330
           E DL+K  G+P     +K  CK++ T+RS   L NNM  E   +V  L ++ +W+ F + 
Sbjct: 275 EIDLEK-TGVPRPDRENK--CKVMFTTRS-IALCNNMGAEYKLRVEFLEKKHAWELFCSK 334

Query: 331 VGEFD---KNRIEHMAKNVVKKCGGLPIALKIIAKALKGKEL-RIWKNALEKLEKRVSVK 390
           V   D    + I  +A+ +V KCGGLP+AL  +  A+  +E    W +A E L  R   +
Sbjct: 335 VWRKDLLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVL-TRFPAE 394

Query: 391 IKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGLLNEVDN 450
           +KG+ + +++ LK SYD +E +  R  FL C++FP++H I  + L  Y +G G L     
Sbjct: 395 MKGM-NYVFALLKFSYDNLESDLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFLTSSHG 454

Query: 451 WETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASNENNMSSL---SYG 510
             T       L+  LK ++ LLE+      V+MH+VVR  A ++AS +     L      
Sbjct: 455 VNTIYKGYF-LIGDLK-AACLLETGDEKTQVKMHNVVRSFALWMASEQGTYKELILVEPS 514

Query: 511 CGQSEWREKDRYGSYNAIFVDCMD--FPNLPKNLEFPNLQMLILRIQTFFGEGQAIQIAD 570
            G +E  + + +    A+ +  +D     LP+ L  P L  L+L+           +I  
Sbjct: 515 MGHTEAPKAENW--RQALVISLLDNRIQTLPEKLICPKLTTLMLQ-----QNSSLKKIPT 574

Query: 571 TFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDILTIFQLKTLEILRI 630
            FF  M  LRVLD+       S TS+T                +  L+I  L  L  L +
Sbjct: 575 GFFMHMPVLRVLDL-------SFTSIT----------------EIPLSIKYLVELYHLSM 634

Query: 631 NGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGGEEVS 690
           +G+ I  LP  + +L +LK LD+     L+ IP + I  ++KLE L+L+ S+  G E  S
Sbjct: 635 SGTKISVLPQELGNLRKLKHLDLQRTQFLQTIPRDAICWLSKLEVLNLYYSY-AGWELQS 680

Query: 691 YKNELIKNVKLSXLNHLTHLSNLMLEIPNVEIL 708
           +  +  + +  + L +L +L+ L + + ++E L
Sbjct: 695 FGEDEAEELGFADLEYLENLTTLGITVLSLETL 680

BLAST of MC02g1309 vs. ExPASy Swiss-Prot
Match: Q9LVT4 (Probable disease resistance protein At5g47250 OS=Arabidopsis thaliana OX=3702 GN=At5g47250 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.3e-48
Identity = 191/674 (28.34%), Postives = 314/674 (46.59%), Query Frame = 0

Query: 20  QLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSYEIYDEVSTWLLAEVNVQLP 79
           ++G +     N+  L+   + LK  +E V   VN G     +    V+TW L++V +   
Sbjct: 22  KVGNICMLKENLVLLKSAFDELKAEKEDVVNRVNAGELKGGQRLAIVATW-LSQVEIIEE 81

Query: 80  NMPQ------------QNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILELVEKGKK 139
           N  Q            QN     R  S   C  +F    L  K  K++ ++  L   GK 
Sbjct: 82  NTKQLMDVASARDASSQNASAVRRRLSTSGC--WFSTCNLGEKVFKKLTEVKSL--SGKD 141

Query: 140 FDKVGYPVPLPHTKSPTLPTDYQILESRTL----LTEKIKDALADPNVNKVGVYGMGGVG 199
           F +V        T+ P  P     L  +T+      EK  ++L       +G++GMGGVG
Sbjct: 142 FQEV--------TEQPPPPVVEVRLCQQTVGLDTTLEKTWESLRKDENRMLGIFGMGGVG 201

Query: 200 KTKWLDEV-KKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNM-GLNMKAETKEGRA 259
           KT  L  +  K V  +  +D VI V   +   V +IQD IG +L++   N    ++  +A
Sbjct: 202 KTTLLTLINNKFVEVSDDYDVVIWVESSKDADVGKIQDAIGERLHICDNNWSTYSRGKKA 261

Query: 260 TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNM 319
           + +   L +M+   ++LLDDLW +  L   +GIP   +      K++ T+RS+ +  + M
Sbjct: 262 SEISRVLRDMKPRFVLLLDDLWEDVSL-TAIGIPVLGKK----YKVVFTTRSKDV-CSVM 321

Query: 320 NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK 379
              +  +V  LSE ++W  F   V     N I  +AK +V KC GLP+AL++I K +  K
Sbjct: 322 RANEDIEVQCLSENDAWDLFDMKVHCDGLNEISDIAKKIVAKCCGLPLALEVIRKTMASK 381

Query: 380 ELRI-WKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEI 439
              I W+ AL+ LE   S ++KG    ++  LKLSYD+++ +  +  FL C++FP  + I
Sbjct: 382 STVIQWRRALDTLESYRS-EMKGTEKGIFQVLKLSYDYLKTKNAK-CFLYCALFPKAYYI 441

Query: 440 CAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 499
              +L  Y +G G ++E D  E  ++   E++D+L  + LLLESN     V MHD++RD+
Sbjct: 442 KQDELVEYWIGEGFIDEKDGRERAKDRGYEIIDNLVGAGLLLESN---KKVYMHDMIRDM 501

Query: 500 AKYIAS----NENNMSSLSYGCGQ----SEWREKDRYGSYNAIFVDCMDFPNLPKNLEFP 559
           A +I S     E  +     G  Q    ++W    +   +N       +  N+P + EFP
Sbjct: 502 ALWIVSEFRDGERYVVKTDAGLSQLPDVTDWTTVTKMSLFN------NEIKNIPDDPEFP 561

Query: 560 NLQMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLC 619
           +   L   +  F    + + I   FF  M  L VLD+      S    +T   K +  L 
Sbjct: 562 DQTNL---VTLFLQNNRLVDIVGKFFLVMSTLVVLDL------SWNFQITELPKGISALV 621

Query: 620 MSYCECDDILTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLI 666
                           +L +L ++G++I+ LP  +  L++L  L++ + S+L  +   LI
Sbjct: 622 ----------------SLRLLNLSGTSIKHLPEGLGVLSKLIHLNLESTSNLRSV--GLI 638

BLAST of MC02g1309 vs. ExPASy Swiss-Prot
Match: Q9LMP6 (Probable disease resistance protein At1g15890 OS=Arabidopsis thaliana OX=3702 GN=At1g15890 PE=3 SV=2)

HSP 1 Score: 191.4 bits (485), Expect = 7.5e-47
Identity = 186/715 (26.01%), Postives = 338/715 (47.27%), Query Frame = 0

Query: 23  YLFFYNRNIKDLEKQLENLKTTREK-VQILVNGRSNSYEIYDEVSTWL--LAEVNVQLPN 82
           Y+     N++ L+  ++ L+  R+  ++ +V       +   +V  WL  + +V  Q+ +
Sbjct: 28  YILKMEANLEALQNTMQELEERRDDLLRRVVIEEDKGLQRLAQVQGWLSRVKDVCSQVND 87

Query: 83  MPQQNKGVSNREASYPSC-LNFFKRHKLSRKSKKRVKDILELVEKGKKFDKVGYPVPLPH 142
           + +     + R      C  NF           K++K +  L+ KG  F+ V   +P P 
Sbjct: 88  LLKAKSIQTERLCLCGYCSKNFISGRNYGINVLKKLKHVEGLLAKG-VFEVVAEKIPAPK 147

Query: 143 TKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGGVGKTKWLDEVKKLVLEN- 202
            +   + T   +      +  +  ++L       +G+YGMGGVGKT  L  +    LE  
Sbjct: 148 VEKKHIQTTVGL----DAMVGRAWNSLMKDERRTLGLYGMGGVGKTTLLASINNKFLEGM 207

Query: 203 KLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILIL 262
             FD VI V + +      IQ+QI G+L +    K  T++ +A+++ N L    +  ++L
Sbjct: 208 NGFDLVIWVVVSKDLQNEGIQEQILGRLGLHRGWKQVTEKEKASYICNIL--NVKKFVLL 267

Query: 263 LDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESW 322
           LDDLW+E DL+K +G+P    + + G KI+ T+RS+ +   +M  +   KV+ L  +E+W
Sbjct: 268 LDDLWSEVDLEK-IGVP--PLTRENGSKIVFTTRSKDV-CRDMEVDGEMKVDCLPPDEAW 327

Query: 323 KFFMTIVGEF---DKNRIEHMAKNVVKKCGGLPIALKIIAKALKGKE-LRIWKNALEKLE 382
           + F   VG         I  +A+ V +KC GLP+AL +I KA+  +E ++ W++ +  L 
Sbjct: 328 ELFQKKVGPIPLQSHEDIPTLARKVAEKCCGLPLALSVIGKAMASRETVQEWQHVIHVLN 387

Query: 383 KRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGL 442
              S +   + +++   LK SYD ++DE+ +L FL CS+FP+D+E+  ++L  Y M  G 
Sbjct: 388 SS-SHEFPSMEEKILPVLKFSYDDLKDEKVKLCFLYCSLFPEDYEVRKEELIEYWMCEGF 447

Query: 443 LNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASN---ENNM 502
           ++  ++ +   N   +++  L  + LL++       V+MHDV+R++A +IASN   +   
Sbjct: 448 IDGNEDEDGANNKGHDIIGSLVRAHLLMDGEL-TTKVKMHDVIREMALWIASNFGKQKET 507

Query: 503 SSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQAI 562
             +  G       +   + S   + + C    N+  +   PNL  L+L+        + +
Sbjct: 508 LCVKPGVQLCHIPKDINWESLRRMSLMCNQIANISSSSNSPNLSTLLLQ------NNKLV 567

Query: 563 QIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDILTIFQLKTLE 622
            I+  FF  M  L VLD+       SR S  +SL                  I +L +L+
Sbjct: 568 HISCDFFRFMPALVVLDL-------SRNSSLSSLPE---------------AISKLGSLQ 627

Query: 623 ILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGG 682
            + ++ + I+ LP +  +L +L  L++    +LE I   + +S+  L+ L L+ S     
Sbjct: 628 YINLSTTGIKWLPVSFKELKKLIHLNLEFTDELESI-VGIATSLPNLQVLKLFSS----- 687

Query: 683 EEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLER-VEKLEEYCI 725
             V     L++ + L     L HL  L   I +  IL     ++R V  ++  C+
Sbjct: 688 -RVCIDGSLMEELLL-----LEHLKVLTATIKDALILESIQGVDRLVSSIQALCL 689

BLAST of MC02g1309 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 1603 bits (4152), Expect = 0.0
Identity = 838/1178 (71.14%), Postives = 956/1178 (81.15%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSY 60
            + ISV AK+ +YTV PIGRQL YLFFYNRNIKDLEKQLENLK TRE+VQ+LVN  RSN+Y
Sbjct: 3    VFISVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSNAY 62

Query: 61   EIYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILE 120
            EIYDEVSTWLL EV+V+LPNMP+Q +  S+R +S  SCLNF +RHKLSRKSKKRVKDIL+
Sbjct: 63   EIYDEVSTWLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDILQ 122

Query: 121  LVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMG 180
            L+E+GKKF+KVG+P PLP TKS TLP DYQ+LESRTL+ EKIKDAL++PNVNKVGV GM 
Sbjct: 123  LIEEGKKFEKVGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGVCGMA 182

Query: 181  GVGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGR 240
            GVGKTK LDE+KKLVLENKLFDRVIDVT+G+SNGVVEIQDQIGGKLNMGL+MKAETKEGR
Sbjct: 183  GVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAETKEGR 242

Query: 241  ATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNN 300
            A FL+NKLVEM++ ILI+LDDLWNEYDL+KEVGIPCRSESSKEGCKILMTSRSR ILTN 
Sbjct: 243  APFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEGCKILMTSRSRDILTNK 302

Query: 301  MNTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKG 360
            MNT+KCF+VNSLSE+ESWKFFM IVGEFDK+RIEH+AKNVVKKCGGLPIALKIIAKALKG
Sbjct: 303  MNTKKCFQVNSLSEDESWKFFMAIVGEFDKSRIEHIAKNVVKKCGGLPIALKIIAKALKG 362

Query: 361  KELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEI 420
            KE+ IWK+A EKLEK V VKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDH I
Sbjct: 363  KEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHNI 422

Query: 421  CAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 480
            C KDLQMYAMGMGLLN+++NWE T+N VI+LVD LKSS LLLESNSGDNYVQMHDVVRDV
Sbjct: 423  CVKDLQMYAMGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDNYVQMHDVVRDV 482

Query: 481  AKYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILR 540
            AKYIASN++ MSSLSYGCGQSEW E+DR GSYNAIFVDCMDF +LP N EFPNLQ+LIL 
Sbjct: 483  AKYIASNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQLLILG 542

Query: 541  IQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDD 600
            + +FFGEG+AIQI   FFEGM+KLRVLDMTKM FE SRTS+T+SLKNLQTLCMSYCE  D
Sbjct: 543  MPSFFGEGEAIQIPGAFFEGMEKLRVLDMTKMCFEPSRTSVTHSLKNLQTLCMSYCEYYD 602

Query: 601  ILTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEE 660
            I TI QLK L+IL+IN  T +ELPA M  LTQL++LD+SNCS+L+VIP N+ISSMTKLEE
Sbjct: 603  IDTIGQLKKLKILKINDCTFEELPANMSQLTQLRLLDLSNCSNLKVIPINIISSMTKLEE 662

Query: 661  LSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLERVEKL 720
            L+LWRSFKR GEE+SY+N+LI+NVKLS LNHL+ LSNL LEIPNV I SD+LSLERVEKL
Sbjct: 663  LNLWRSFKRWGEEISYRNKLIQNVKLSELNHLSRLSNLRLEIPNVNIFSDDLSLERVEKL 722

Query: 721  EEYCICVG-DNEDFEICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRVKDSVGFINH 780
            EE+CICVG D+  F ICD YAR L+VNIKSQIV+ GG L+ILLEGCEQL ++D+VGF N 
Sbjct: 723  EEFCICVGNDSWGFRICDGYARRLMVNIKSQIVAIGGTLQILLEGCEQLYIEDTVGFTNC 782

Query: 781  LFKPNGNYCYPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIY 840
            LFK NGNYC PR+K+LHIEGNS+MPR ID QV     +                      
Sbjct: 783  LFKLNGNYCNPRLKHLHIEGNSEMPRRIDVQVSFPELKE--------------------- 842

Query: 841  LFTTHFRNSITLLRKKQ--------KLQTIRIQYCNKLKHAFPSNVVTSLVFLNILEILH 900
              T    N++ +L            KL++I I  CNKL + F SN+VTSLVFLN LEI +
Sbjct: 843  -LTIQGGNNLEMLWHNNRLIANSFCKLRSICISCCNKLTYMFTSNMVTSLVFLNTLEIDN 902

Query: 901  CALLEGIFEVDMLTDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPH 960
            C LLE IFE+  L D+AKV+PL +L L SLP LKYVWN DP EF+TFPNL+ V VS CP 
Sbjct: 903  CELLERIFEIGKLVDDAKVLPLTDLSLRSLPNLKYVWNNDPGEFLTFPNLKNVNVSSCPR 962

Query: 961  LKTIFPTSFIKHMDEIENLVTDEVNEIFPEDEASKLVAPEVMHLRHYLLFTQYFVYVSIC 1020
            LK +FP SFIKH++EIE+L   EV+EIF EDEASKL  PE                    
Sbjct: 963  LKCLFPASFIKHIEEIESLCAYEVDEIFSEDEASKLGFPE-------------------- 1022

Query: 1021 FVDFQIVFPSLKNLKMETKRSFWFRSRSFPKLQGLVLNGFEDENMVTLPLEMSQEINLVR 1080
                 IVF SL+ L+M  KRSFWFRS SFPKL  L L G ED+++VTLPLEMSQE+  + 
Sbjct: 1023 -----IVFRSLQILEMVVKRSFWFRSGSFPKLHNLKLKGGEDDDLVTLPLEMSQELYNIE 1082

Query: 1081 VKNCDKLKCLLPLSVSFFHLKFLTIWDCNGMINLFSSSVAKNLVSLSCIKISNCRGMSSI 1140
            VK CDKLK LLP S +FF+L  L I DCNGM+NLF++ VAKNLV+L  IKI +CRG++SI
Sbjct: 1083 VKKCDKLKFLLPSSTTFFNLGSLIIEDCNGMMNLFNTLVAKNLVNLCLIKICDCRGITSI 1131

Query: 1141 VATEEEEEEEKDEEIILKKLTDLELDDLPRLANFYSGK 1168
            VA  EE EEE+ EEII   L  LEL DLPRLANFYSGK
Sbjct: 1143 VA--EEVEEEEGEEIIFNHLIRLELRDLPRLANFYSGK 1131

BLAST of MC02g1309 vs. NCBI nr
Match: XP_022155050.1 (disease resistance protein RPS5-like [Momordica charantia])

HSP 1 Score: 1431 bits (3705), Expect = 0.0
Identity = 845/1552 (54.45%), Postives = 891/1552 (57.41%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSY 60
            ILIS+VAK+AEYTV PIGRQLGYLFFY++NIKD+EKQLENLKTTREKVQILVN  RSN+Y
Sbjct: 3    ILISIVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSNAY 62

Query: 61   EIYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILE 120
            EIYDEVSTWLLAEV+V+L NMP +NKGVSNR+ S  SCLNF +RHKLSR+SKKRVKDILE
Sbjct: 63   EIYDEVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDILE 122

Query: 121  LVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMG 180
            ++EKGKKFDKVGYP PLP TKS TLP DYQILESRTLL EKIKD+LADPNVNKVGV GMG
Sbjct: 123  IIEKGKKFDKVGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCGMG 182

Query: 181  GVGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGR 240
            GVGKTK LDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKA TKEGR
Sbjct: 183  GVGKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKEGR 242

Query: 241  ATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNN 300
            A FL+NKLVEM+++ILI+LDDLW+EYDLQKEVGIPCRS SSKEGCKILMTSRSR ILTN 
Sbjct: 243  APFLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCRSRSSKEGCKILMTSRSRDILTNR 302

Query: 301  MNTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKG 360
            M+ EKCF+VNSLSEEESWKFF+ IVGEFDKNRIEH+A+NVVKKCGGLPIALKIIAKALKG
Sbjct: 303  MDAEKCFEVNSLSEEESWKFFIAIVGEFDKNRIEHLARNVVKKCGGLPIALKIIAKALKG 362

Query: 361  KELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEI 420
            K++RIWK+ALEKLEKRVSVKIKGVSDQLYSCLKLSYD IEDEETRLLFLLCSVFPDDH+I
Sbjct: 363  KDVRIWKDALEKLEKRVSVKIKGVSDQLYSCLKLSYDRIEDEETRLLFLLCSVFPDDHKI 422

Query: 421  CAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 480
              KDLQMYAMGMGLLN++DNWET+RNMV+ELVDHLKSSSLLLESNSGDNYVQMHDVVRDV
Sbjct: 423  HVKDLQMYAMGMGLLNDIDNWETSRNMVVELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 482

Query: 481  AKYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILR 540
            AKYIA+ EN MSSLSYG GQSEWREK+R GSYN IF+DC+DFPNLP N+EFP+LQMLILR
Sbjct: 483  AKYIATKENKMSSLSYGRGQSEWREKERCGSYNVIFIDCVDFPNLPPNMEFPDLQMLILR 542

Query: 541  IQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDD 600
            IQ+FFGEG+AIQI +  FEGMKKLRVLDMTKM FE S+TS T+SLKNLQTL MSYCEC+D
Sbjct: 543  IQSFFGEGRAIQIPEALFEGMKKLRVLDMTKMCFEPSKTSFTHSLKNLQTLRMSYCECND 602

Query: 601  ILTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEE 660
            ILTIFQLK LEILRING                                           
Sbjct: 603  ILTIFQLKKLEILRING------------------------------------------- 662

Query: 661  LSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLERVEKL 720
                                                                        
Sbjct: 663  ------------------------------------------------------------ 722

Query: 721  EEYCICVGDNEDFEICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRVKDSVGFINHL 780
                                                                        
Sbjct: 723  ------------------------------------------------------------ 782

Query: 781  FKPNGNYCYPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIYL 840
                                                                        
Sbjct: 783  ------------------------------------------------------------ 842

Query: 841  FTTHFRNSITLLRKKQKLQTIRIQYCNKLKHAFPSNVVTSLVFLNILEILHCALLEGIFE 900
                                                                        
Sbjct: 843  ------------------------------------------------------------ 902

Query: 901  VDMLTDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPHLKTIFPTSF 960
                                                                        
Sbjct: 903  ------------------------------------------------------------ 933

Query: 961  IKHMDEIENLVTDEVNEIFPEDEASKLVAPEVMHLRHYLLFTQYFVYVSICFVDFQIVFP 1020
                                                                        
Sbjct: 963  ------------------------------------------------------------ 933

Query: 1021 SLKNLKMETKRSFWFRSRSFPKLQGLVLNGFEDENMVTLPLEMSQEINLVRVKNCDKLKC 1080
                                                                        
Sbjct: 1023 ------------------------------------------------------------ 933

Query: 1081 LLPLSVSFFHLKFLTIWDCNGMINLFSSSVAKNLVSLSCIKISNCRGMSSIVATEEEEEE 1140
                                                                        
Sbjct: 1083 ------------------------------------------------------------ 933

Query: 1141 EKDEEIILKKLTDLELDDLPRLANFYSGKYSFNGTLSHDDDSELYLSHMYWINGIMIITL 1200
                                                                  IMIITL
Sbjct: 1143 ------------------------------------------------------IMIITL 933

Query: 1201 QVSFPELEDLRSEGLSLETLWHNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFL 1260
            QVSFPELEDLRSEGLSLETLWHNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFL
Sbjct: 1203 QVSFPELEDLRSEGLSLETLWHNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFL 933

Query: 1261 KILEIYTCELLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRK 1320
            KILEIYTCELLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRK
Sbjct: 1263 KILEIYTCELLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRK 933

Query: 1321 VEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPEVMHLFHYYSLAQHLYMFIFGT 1380
            VEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPE                     
Sbjct: 1323 VEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPE--------------------- 933

Query: 1381 TFEDFHDTRVIKIIVXKLLFYFQMVLSKGFGDENIVTLSVETSQESNFIQVKKCDKMNCL 1440
                                   MVLSKGFGDENIVTLSVETSQESNFIQVKKCDKMNCL
Sbjct: 1383 -----------------------MVLSKGFGDENIVTLSVETSQESNFIQVKKCDKMNCL 933

Query: 1441 LPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKD 1500
            LPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKD
Sbjct: 1443 LPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKD 933

Query: 1501 EEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI 1551
            EEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI
Sbjct: 1503 EEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI 933

BLAST of MC02g1309 vs. NCBI nr
Match: XP_022155051.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 572/686 (83.38%), Postives = 577/686 (84.11%), Query Frame = 0

Query: 1   ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVNGRSNSYE 60
           ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTT EKVQILVN       
Sbjct: 3   ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTGEKVQILVNEE----- 62

Query: 61  IYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILEL 120
                          ++ +M    K   +R   +P                         
Sbjct: 63  ---------------EVIHMKSMTK---SRHGCWPKS----------------------- 122

Query: 121 VEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGG 180
                                                           NVNKVGVYGMGG
Sbjct: 123 ------------------------------------------------NVNKVGVYGMGG 182

Query: 181 VGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRA 240
           VGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRA
Sbjct: 183 VGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRA 242

Query: 241 TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNM 300
           TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSR ILTNNM
Sbjct: 243 TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRHILTNNM 302

Query: 301 NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK 360
           NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK
Sbjct: 303 NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK 362

Query: 361 ELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEIC 420
           ELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEIC
Sbjct: 363 ELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEIC 422

Query: 421 AKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA 480
           AKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA
Sbjct: 423 AKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA 482

Query: 481 KYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRI 540
           KYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRI
Sbjct: 483 KYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRI 542

Query: 541 QTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDI 600
           QTFFGEGQAIQI DTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDI
Sbjct: 543 QTFFGEGQAIQIPDTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDI 594

Query: 601 LTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEEL 660
           LTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEEL
Sbjct: 603 LTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEEL 594

Query: 661 SLWRSFKRGGEEVSYKNELIKNVKLS 686
           SLWRSFKRGGEEVSYKNELIKNVKLS
Sbjct: 663 SLWRSFKRGGEEVSYKNELIKNVKLS 594

BLAST of MC02g1309 vs. NCBI nr
Match: XP_038887230.1 (probable disease resistance protein At5g63020 [Benincasa hispida])

HSP 1 Score: 983 bits (2541), Expect = 0.0
Identity = 623/1275 (48.86%), Postives = 777/1275 (60.94%), Query Frame = 0

Query: 4    SVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN---GRSNSYE 63
            +VV  +A+YT++PI  QL YLF Y  NIK++EK+L  L+TT   VQILVN   G+ N+  
Sbjct: 75   AVVGVLADYTIKPILHQLDYLFHYKGNIKEVEKKLGILRTTERTVQILVNQDKGKGNA-- 134

Query: 64   IYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILEL 123
            I+D V+ WL    NV   +M QQN      EA  PSC N  +RH+LSRK+KKR++DI EL
Sbjct: 135  IFDGVTNWLDNVKNVL--DMAQQN------EAPNPSCFNCVQRHQLSRKAKKRLEDINEL 194

Query: 124  VEKGKKFDK--VGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGM 183
            + +G++F+K  VGYPVP P T S TLP DYQ  ESRT + EKIKDALA+PNVNKVGV GM
Sbjct: 195  INEGRQFNKDNVGYPVPSPDTSSSTLPADYQFFESRTSMAEKIKDALANPNVNKVGVCGM 254

Query: 184  GGVGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMG-LNMKAETKE 243
            GGVGKT  L EVKKLVLENKLFDRVI V +GQS  V+ IQ++I  KLNM  LNM+ E  E
Sbjct: 255  GGVGKTTLLKEVKKLVLENKLFDRVIQVEVGQSKSVIHIQEEIRAKLNMEYLNMEFE--E 314

Query: 244  GRATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILT 303
             R + LQ  + E +ENIL +LDDLW EYD++KE GIP RS+S  EGCKILMTSR R + T
Sbjct: 315  VRTSRLQTHIAEKKENILFMLDDLWKEYDVEKEFGIPYRSDSRIEGCKILMTSRRRDLFT 374

Query: 304  NNMNTEKCFKVNSLSEEESWKFFMTIVGE--FDKNRIEHMAKNVVKKCGGLPIALKIIAK 363
            N MN EK F+VNSL++EESWKFF  I+G+   +   ++ +AK V+ +CGGLPIALKI+ K
Sbjct: 375  NQMNIEKLFEVNSLTKEESWKFFSNIIGDEFVEDGYMKQIAKYVINECGGLPIALKILGK 434

Query: 364  ALKGKELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPD 423
             LKG+ + IWK+AL+KL+  V V I GVSDQLYSCL+ SYD IE+E  +LL LLCSVFPD
Sbjct: 435  TLKGRRVEIWKDALKKLKNPVVVDIHGVSDQLYSCLQFSYDSIENE-AKLLLLLCSVFPD 494

Query: 424  DHEICAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNS-GDNYVQMHD 483
            DH I   DLQ YAMGMGLLN+ + WE  +N VI+LVD LKSS LLL      DNYV+MHD
Sbjct: 495  DHRIYVDDLQKYAMGMGLLNQTNTWEDAKNRVIKLVDDLKSSYLLLAPPEWDDNYVKMHD 554

Query: 484  VVRDVAKYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQ 543
            VV D AKYIAS  + M+S +YG GQ  W E +R+GSYNAI+VD   F NLP  LEFPNLQ
Sbjct: 555  VVHDFAKYIASKIDKMTSFTYGNGQRAWEEDNRHGSYNAIYVDPTTFLNLPPKLEFPNLQ 614

Query: 544  MLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSY 603
            + IL I    G    IQ  +T FEGMK+LRVLD+    FE S   L  SLKNL+ LC+S+
Sbjct: 615  LFIL-ISNGLGPRPTIQNLETVFEGMKELRVLDVRMWYFEPS---LIYSLKNLRVLCLSH 674

Query: 604  CECDDILTIFQLKTLEILRINGST-IQELPATMDDLTQLKVLDVSNCSDLEVIPANLISS 663
            C+ +DI  I +LK L+IL I+    IQE P     LTQLKVL VS+C +L+VI  N+IS 
Sbjct: 675  CDSNDIDAIGELKRLKILEIHFCRGIQEFPECTSKLTQLKVLQVSHCMNLKVIHPNIISK 734

Query: 664  MTKLEELSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLML-EIPNVEILSDNLS 723
            MT+LE+LS   SF   GEEV YK +LI+N KLS LN LTHL +L L  + NVEILS+ LS
Sbjct: 735  MTRLEDLSFSHSFDGWGEEVWYKQKLIRNAKLSELNCLTHLVHLELCNLKNVEILSNELS 794

Query: 724  LERVEKLEEYCICVGD----------NEDFEICDE-YARSLVVNIKSQIVSNGGVLEILL 783
            LE  +KLE   I VGD          N  +   D+ Y  +L + I S I S GGV +ILL
Sbjct: 795  LESTQKLETCRIFVGDERWNIHYNDRNNPYNRKDKRYTTALNLYIDSPIDSIGGVFQILL 854

Query: 784  EGCEQLRVKDSVGFINHLFKPNGNYCYPRMKNLHIE------GNSDMPRHIDFQVPAKAF 843
            + CE+L ++DSVGF   L     N CYP +K+L I+      GN   P + D QV     
Sbjct: 855  QKCEKLVLRDSVGFTMLLCNTYANSCYPLLKHLEIDYPPAIIGNIS-PLNFDLQVAFPQL 914

Query: 844  RLFFFFINFTFICCFKKIYIY-IYLFTTHFRNSITLLRKKQKLQTIRIQYCNKLKHAFPS 903
                           K +YI     F T +RN+        KL+ I I+ CNK  + FPS
Sbjct: 915  ---------------KSLYIGGRNNFETLWRNNGLTENYFSKLERIFIEGCNKFTYVFPS 974

Query: 904  NVVTSLVFLNILEILHCALLEGIFEVDMLTDEA--KVVPLKNLILISLPKLKYVWNKDPD 963
             +V SLVFLN L + +C L+E IFE++  T     + VPL  L L SLP LKYVWN DP 
Sbjct: 975  RMVRSLVFLNRLVVRYCELVERIFEIEESTSIIVNQDVPLTILELSSLPNLKYVWNNDPA 1034

Query: 964  -EFMTFPNLRQVEVSRCPHLKTIFPTSFIKHMDEIENLVTDEVNEIFPE-DEASKLVAPE 1023
             E +TFPNL++V+V  CP LKT+FP SFI HM E E L      EIF E DEAS+LV+P+
Sbjct: 1035 LELLTFPNLKKVKVEECPQLKTLFPVSFINHMKEFEELDCVGETEIFGETDEASQLVSPQ 1094

Query: 1024 VMHLRHYLLFTQYFVYVSICFVDFQIVFPSLKNLKME----TKRSFWFRSRSFPKLQGLV 1083
            +                          FPSL+ L +      +RSFWFR  SF +L  L 
Sbjct: 1095 I--------------------------FPSLRYLTVHYTLMKRRSFWFRPESFSELLTLR 1154

Query: 1084 LNGFEDENMVTLPLEMSQE----------------------------------------- 1143
            L G ED++MV+LP EMS+                                          
Sbjct: 1155 LIGSEDDDMVSLPFEMSESAVWRSVVRHLQIENAFEWVKIFRNGEENNQNYHASSSGGAK 1214

Query: 1144 --------------------------------INLVRVKNCDKLKCLLPLSVSFFHLKFL 1168
                                            +  + V  CD LKCLLP S++F +L FL
Sbjct: 1215 LKHLCLYNLPKLTHIWEDSIDQMTATSCFVDILESIVVDRCDNLKCLLPSSITFSNLTFL 1274

BLAST of MC02g1309 vs. NCBI nr
Match: XP_038902150.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 632/1569 (40.28%), Postives = 856/1569 (54.56%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSY 60
            +L+SVVA     T++PIG QLGYL  YN+N K+L++Q+ENL+TT+  V   V   +  +Y
Sbjct: 3    VLVSVVAA----TIKPIGHQLGYLVCYNKNKKELQEQIENLETTKRDVDKRVEEAKGKAY 62

Query: 61   EIYDEVSTWLL----AEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVK 120
             + DEVS WL     A ++ QL              +S PSC N  +R++LSR++KK+V 
Sbjct: 63   TVSDEVSKWLTDVDGAMIHHQL-------------SSSNPSCFNLAQRYRLSRETKKKVN 122

Query: 121  DILELVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGV 180
             IL+L++K  KF +VGY  PL  T++  +P DYQ+LES+T L + IK+ALA P+VNKVGV
Sbjct: 123  YILQLIDKRNKFLEVGYRAPLADTENTIVPGDYQVLESKTSLAKDIKNALAKPDVNKVGV 182

Query: 181  YGMGGVGKTKWLDEVKKLVL--ENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKA 240
            YGMGGVGKT  L+EVK+LVL  ++K FDRVIDV++G+SN + +IQ+QIG +LN+ L    
Sbjct: 183  YGMGGVGKTYLLNEVKRLVLKEDDKSFDRVIDVSVGRSNDLTQIQEQIGDQLNIEL---P 242

Query: 241  ETKEGRATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSR 300
            ++KEGRA+FL+N L +M  NIL+LLDDLW EYDL KE+GIP     SKEGCK+LMTSRSR
Sbjct: 243  KSKEGRASFLRNNLAKMEGNILVLLDDLWKEYDLIKEIGIPL----SKEGCKVLMTSRSR 302

Query: 301  RILTNNMNTEKCFKVNSLSEEESWKFFMTIVGE-FDKNRIEHMAKNVVKKCGGLPIALKI 360
             ILTNNMNT++CF+V+SL+EEESWKFF  I+G+ FD   +E +AK V K+CGGLP+AL  
Sbjct: 303  DILTNNMNTQECFQVSSLTEEESWKFFKAIIGDKFDTIYMESIAKEVSKECGGLPLALDT 362

Query: 361  IAKALKGKELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSV 420
            IAKALKGK++  W++AL KL+  + + IKGVSD++Y+ L+LSY++++ EET+LLFLLCSV
Sbjct: 363  IAKALKGKDMHYWEDALSKLKNSIGMDIKGVSDKVYASLRLSYEYLDGEETKLLFLLCSV 422

Query: 421  FPDDHEICAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLE--SNSGDNYV 480
            FPDD++I  KDLQMYAMGM LLN+V  WE  +N V++LV  L SSSL LE  S+S D YV
Sbjct: 423  FPDDYKIGMKDLQMYAMGMRLLNKVKTWEDAKNRVMKLVHDLISSSLFLEADSDSKDKYV 482

Query: 481  QMHDVVRDVAKYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEF 540
            +MHDVVRDVA +IASNE NMS+L+ G   +EW ++ R GS+ AIF +C +  NLP  ++F
Sbjct: 483  KMHDVVRDVAIHIASNEGNMSTLNIGYKLNEWEDEYRSGSHRAIFANCDNLNNLPPKMKF 542

Query: 541  PNLQMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTL 600
            P L++LILR+  +  E   +QI   FFEGM+KL+VLDMT M F     + T+SL NL+TL
Sbjct: 543  PQLELLILRVSNWLVENN-LQIPYAFFEGMEKLKVLDMTGMCFHRPLWT-TSSLNNLRTL 602

Query: 601  CMSYCECDDILTIFQLKTLEILRI-NGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPAN 660
            CM  CE +DI TI +LK LEILRI   + +  LP TM  LT LKVL+V NCS LEV+PAN
Sbjct: 603  CMVCCEFNDIDTIGELKKLEILRIMKCNMLDHLPTTMSQLTHLKVLEVLNCSKLEVVPAN 662

Query: 661  LISSMTKLEELSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSD 720
            + SSMTKLEEL L  SF R GEEV YK+ELIKNVKLS LN+L  LSNL LE   V+ILS+
Sbjct: 663  IFSSMTKLEELKLQDSFCRWGEEVWYKDELIKNVKLSELNYLPRLSNLCLESWKVKILSE 722

Query: 721  NLSLERVEKLEEYCICVGDNEDF---EICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQ 780
             +S +  + L+E+ IC  +++D    ++ +EYA +L++NI+SQI S  G LEILL+  E+
Sbjct: 723  -ISSQTCKNLKEFWICSNESDDIIQPKVSNEYATTLMLNIESQIGSIDGGLEILLQRSER 782

Query: 781  LRVKDSVG-FINHLFKPNGNYCYPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFT 840
            L V DS+G F+N +FKPNGN                                        
Sbjct: 783  LIVSDSMGNFVNGIFKPNGN---------------------------------------- 842

Query: 841  FICCFKKIYIYIYLFTTHFRNSITLLRKKQKLQTIRIQYCNKLKHAFPSNVVTSLVFLNI 900
                                                                        
Sbjct: 843  ------------------------------------------------------------ 902

Query: 901  LEILHCALLEGIFEVDMLTDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEV 960
                                               P LKY+W                  
Sbjct: 903  ---------------------------------GFPLLKYLWI----------------- 962

Query: 961  SRCPHLKTIFPTSFIKHMDEIENLVTDEVNEIFPEDEASKLVAPEVMHLRHYLLFTQYFV 1020
                             +DE  N             E   L+  +   L++ +L+     
Sbjct: 963  -----------------IDEHSN------------SEMPHLIGSDFTSLKYLILY----- 1022

Query: 1021 YVSICFVDFQIVFPSLKNLKMETKRSFWFRSRSFPKLQGLVLNGFEDENMVTLPLEMSQE 1080
                           ++ L                            EN+V   + +S  
Sbjct: 1023 --------------GMRRL----------------------------ENIVPKHITIS-- 1082

Query: 1081 INLVRVKNCDKLKCLLPLSVSFFHLKFLTIWDCNGMINLFSSSVAKNLVSLSCIKISNCR 1140
                                 F  LK + I  C  + NLFS S+ K L  L  I++ NC 
Sbjct: 1083 --------------------PFKKLKTIAIQFCGQLRNLFSFSIFKGLSDLQEIEVINCG 1142

Query: 1141 GMSSIVATEEEEEEEKDEEIILKKLTDLELDDLPRLANFYSGKYSFNGTLSHDDDSELYL 1200
             M  I+  E E++      I    L  L L+++ +L++F +      G +     S L  
Sbjct: 1143 MMDEIIFMEIEDQPN----ICTTPLASLILENVDKLSSFCT-----KGLIQESPQSIL-- 1194

Query: 1201 SHMYWINGIMIITLQVSFPELEDLRSEGL-SLETLWH-NNGLSTHPFCKLQNIRIQYCNK 1260
                          QV FPEL DL   G  +LETLWH NN  +T+ FCKLQ+IRI+ C K
Sbjct: 1203 ---------PFFDQQVLFPELNDLSIIGSKNLETLWHKNNEPTTNSFCKLQSIRIEMCTK 1194

Query: 1261 LKYVFPSNVVTSLVFLKILEIYTCELLEGIFEVEKLT-DEARATVPLTKLILISLPKLKY 1320
            ++ +FPSN+VTSLV L  +EI +C  L+ IFE+EK +  + +    L +L L++LP LK+
Sbjct: 1263 IRCMFPSNMVTSLVSLDTIEITSCASLKRIFEIEKESFGDTKVAWSLRELHLLNLPNLKH 1194

Query: 1321 VWDKDPGEFLTFPNLRKVEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPEVMHL 1380
            VW KD  EF+ FP+L++V++ GC +L          H+ +  T +T              
Sbjct: 1323 VWRKDVIEFMKFPSLKRVKIHGCTKLA---------HIWKENTKMT-------------- 1194

Query: 1381 FHYYSLAQHLYMFIFGTTFEDFHDTRVIKIIVXKLLFYFQMVLSKGFGDENIVTLSVETS 1440
                            T+F+                                        
Sbjct: 1383 ----------------TSFDSLES------------------------------------ 1194

Query: 1441 QESNFIQVKKCDKMNCLLPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISN 1500
                 I+V++C+ +  LLPSSI+F NLN L + ECNGM+NLFSS+VAK+LV+L  + +S 
Sbjct: 1443 -----IEVERCENLKYLLPSSITFLNLNELHVKECNGMMNLFSSTVAKNLVNLRSIEVSY 1194

Query: 1501 CEEMSSIIAREEEEEKDEEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCP 1551
            CE MS I+  E +EE DE +IFKKL  LE D LPRL +FYSGK  L+FP L +L I +CP
Sbjct: 1503 CEGMSCIV--EAKEENDESVIFKKLGKLEFDCLPRLDSFYSGKCMLEFPSLESLVIKRCP 1194

BLAST of MC02g1309 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 1603 bits (4152), Expect = 0.0
Identity = 838/1178 (71.14%), Postives = 956/1178 (81.15%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSY 60
            + ISV AK+ +YTV PIGRQL YLFFYNRNIKDLEKQLENLK TRE+VQ+LVN  RSN+Y
Sbjct: 3    VFISVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSNAY 62

Query: 61   EIYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILE 120
            EIYDEVSTWLL EV+V+LPNMP+Q +  S+R +S  SCLNF +RHKLSRKSKKRVKDIL+
Sbjct: 63   EIYDEVSTWLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDILQ 122

Query: 121  LVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMG 180
            L+E+GKKF+KVG+P PLP TKS TLP DYQ+LESRTL+ EKIKDAL++PNVNKVGV GM 
Sbjct: 123  LIEEGKKFEKVGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGVCGMA 182

Query: 181  GVGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGR 240
            GVGKTK LDE+KKLVLENKLFDRVIDVT+G+SNGVVEIQDQIGGKLNMGL+MKAETKEGR
Sbjct: 183  GVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAETKEGR 242

Query: 241  ATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNN 300
            A FL+NKLVEM++ ILI+LDDLWNEYDL+KEVGIPCRSESSKEGCKILMTSRSR ILTN 
Sbjct: 243  APFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEGCKILMTSRSRDILTNK 302

Query: 301  MNTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKG 360
            MNT+KCF+VNSLSE+ESWKFFM IVGEFDK+RIEH+AKNVVKKCGGLPIALKIIAKALKG
Sbjct: 303  MNTKKCFQVNSLSEDESWKFFMAIVGEFDKSRIEHIAKNVVKKCGGLPIALKIIAKALKG 362

Query: 361  KELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEI 420
            KE+ IWK+A EKLEK V VKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDH I
Sbjct: 363  KEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHNI 422

Query: 421  CAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 480
            C KDLQMYAMGMGLLN+++NWE T+N VI+LVD LKSS LLLESNSGDNYVQMHDVVRDV
Sbjct: 423  CVKDLQMYAMGMGLLNDINNWEATKNRVIDLVDDLKSSYLLLESNSGDNYVQMHDVVRDV 482

Query: 481  AKYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILR 540
            AKYIASN++ MSSLSYGCGQSEW E+DR GSYNAIFVDCMDF +LP N EFPNLQ+LIL 
Sbjct: 483  AKYIASNDDKMSSLSYGCGQSEWLEEDRSGSYNAIFVDCMDFSSLPSNFEFPNLQLLILG 542

Query: 541  IQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDD 600
            + +FFGEG+AIQI   FFEGM+KLRVLDMTKM FE SRTS+T+SLKNLQTLCMSYCE  D
Sbjct: 543  MPSFFGEGEAIQIPGAFFEGMEKLRVLDMTKMCFEPSRTSVTHSLKNLQTLCMSYCEYYD 602

Query: 601  ILTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEE 660
            I TI QLK L+IL+IN  T +ELPA M  LTQL++LD+SNCS+L+VIP N+ISSMTKLEE
Sbjct: 603  IDTIGQLKKLKILKINDCTFEELPANMSQLTQLRLLDLSNCSNLKVIPINIISSMTKLEE 662

Query: 661  LSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLERVEKL 720
            L+LWRSFKR GEE+SY+N+LI+NVKLS LNHL+ LSNL LEIPNV I SD+LSLERVEKL
Sbjct: 663  LNLWRSFKRWGEEISYRNKLIQNVKLSELNHLSRLSNLRLEIPNVNIFSDDLSLERVEKL 722

Query: 721  EEYCICVG-DNEDFEICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRVKDSVGFINH 780
            EE+CICVG D+  F ICD YAR L+VNIKSQIV+ GG L+ILLEGCEQL ++D+VGF N 
Sbjct: 723  EEFCICVGNDSWGFRICDGYARRLMVNIKSQIVAIGGTLQILLEGCEQLYIEDTVGFTNC 782

Query: 781  LFKPNGNYCYPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIY 840
            LFK NGNYC PR+K+LHIEGNS+MPR ID QV     +                      
Sbjct: 783  LFKLNGNYCNPRLKHLHIEGNSEMPRRIDVQVSFPELKE--------------------- 842

Query: 841  LFTTHFRNSITLLRKKQ--------KLQTIRIQYCNKLKHAFPSNVVTSLVFLNILEILH 900
              T    N++ +L            KL++I I  CNKL + F SN+VTSLVFLN LEI +
Sbjct: 843  -LTIQGGNNLEMLWHNNRLIANSFCKLRSICISCCNKLTYMFTSNMVTSLVFLNTLEIDN 902

Query: 901  CALLEGIFEVDMLTDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPH 960
            C LLE IFE+  L D+AKV+PL +L L SLP LKYVWN DP EF+TFPNL+ V VS CP 
Sbjct: 903  CELLERIFEIGKLVDDAKVLPLTDLSLRSLPNLKYVWNNDPGEFLTFPNLKNVNVSSCPR 962

Query: 961  LKTIFPTSFIKHMDEIENLVTDEVNEIFPEDEASKLVAPEVMHLRHYLLFTQYFVYVSIC 1020
            LK +FP SFIKH++EIE+L   EV+EIF EDEASKL  PE                    
Sbjct: 963  LKCLFPASFIKHIEEIESLCAYEVDEIFSEDEASKLGFPE-------------------- 1022

Query: 1021 FVDFQIVFPSLKNLKMETKRSFWFRSRSFPKLQGLVLNGFEDENMVTLPLEMSQEINLVR 1080
                 IVF SL+ L+M  KRSFWFRS SFPKL  L L G ED+++VTLPLEMSQE+  + 
Sbjct: 1023 -----IVFRSLQILEMVVKRSFWFRSGSFPKLHNLKLKGGEDDDLVTLPLEMSQELYNIE 1082

Query: 1081 VKNCDKLKCLLPLSVSFFHLKFLTIWDCNGMINLFSSSVAKNLVSLSCIKISNCRGMSSI 1140
            VK CDKLK LLP S +FF+L  L I DCNGM+NLF++ VAKNLV+L  IKI +CRG++SI
Sbjct: 1083 VKKCDKLKFLLPSSTTFFNLGSLIIEDCNGMMNLFNTLVAKNLVNLCLIKICDCRGITSI 1131

Query: 1141 VATEEEEEEEKDEEIILKKLTDLELDDLPRLANFYSGK 1168
            VA  EE EEE+ EEII   L  LEL DLPRLANFYSGK
Sbjct: 1143 VA--EEVEEEEGEEIIFNHLIRLELRDLPRLANFYSGK 1131

BLAST of MC02g1309 vs. ExPASy TrEMBL
Match: A0A6J1DLB8 (disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022189 PE=4 SV=1)

HSP 1 Score: 1431 bits (3705), Expect = 0.0
Identity = 845/1552 (54.45%), Postives = 891/1552 (57.41%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSY 60
            ILIS+VAK+AEYTV PIGRQLGYLFFY++NIKD+EKQLENLKTTREKVQILVN  RSN+Y
Sbjct: 3    ILISIVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSNAY 62

Query: 61   EIYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILE 120
            EIYDEVSTWLLAEV+V+L NMP +NKGVSNR+ S  SCLNF +RHKLSR+SKKRVKDILE
Sbjct: 63   EIYDEVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDILE 122

Query: 121  LVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMG 180
            ++EKGKKFDKVGYP PLP TKS TLP DYQILESRTLL EKIKD+LADPNVNKVGV GMG
Sbjct: 123  IIEKGKKFDKVGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCGMG 182

Query: 181  GVGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGR 240
            GVGKTK LDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKA TKEGR
Sbjct: 183  GVGKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKEGR 242

Query: 241  ATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNN 300
            A FL+NKLVEM+++ILI+LDDLW+EYDLQKEVGIPCRS SSKEGCKILMTSRSR ILTN 
Sbjct: 243  APFLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCRSRSSKEGCKILMTSRSRDILTNR 302

Query: 301  MNTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKG 360
            M+ EKCF+VNSLSEEESWKFF+ IVGEFDKNRIEH+A+NVVKKCGGLPIALKIIAKALKG
Sbjct: 303  MDAEKCFEVNSLSEEESWKFFIAIVGEFDKNRIEHLARNVVKKCGGLPIALKIIAKALKG 362

Query: 361  KELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEI 420
            K++RIWK+ALEKLEKRVSVKIKGVSDQLYSCLKLSYD IEDEETRLLFLLCSVFPDDH+I
Sbjct: 363  KDVRIWKDALEKLEKRVSVKIKGVSDQLYSCLKLSYDRIEDEETRLLFLLCSVFPDDHKI 422

Query: 421  CAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 480
              KDLQMYAMGMGLLN++DNWET+RNMV+ELVDHLKSSSLLLESNSGDNYVQMHDVVRDV
Sbjct: 423  HVKDLQMYAMGMGLLNDIDNWETSRNMVVELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 482

Query: 481  AKYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILR 540
            AKYIA+ EN MSSLSYG GQSEWREK+R GSYN IF+DC+DFPNLP N+EFP+LQMLILR
Sbjct: 483  AKYIATKENKMSSLSYGRGQSEWREKERCGSYNVIFIDCVDFPNLPPNMEFPDLQMLILR 542

Query: 541  IQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDD 600
            IQ+FFGEG+AIQI +  FEGMKKLRVLDMTKM FE S+TS T+SLKNLQTL MSYCEC+D
Sbjct: 543  IQSFFGEGRAIQIPEALFEGMKKLRVLDMTKMCFEPSKTSFTHSLKNLQTLRMSYCECND 602

Query: 601  ILTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEE 660
            ILTIFQLK LEILRING                                           
Sbjct: 603  ILTIFQLKKLEILRING------------------------------------------- 662

Query: 661  LSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLERVEKL 720
                                                                        
Sbjct: 663  ------------------------------------------------------------ 722

Query: 721  EEYCICVGDNEDFEICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRVKDSVGFINHL 780
                                                                        
Sbjct: 723  ------------------------------------------------------------ 782

Query: 781  FKPNGNYCYPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIYL 840
                                                                        
Sbjct: 783  ------------------------------------------------------------ 842

Query: 841  FTTHFRNSITLLRKKQKLQTIRIQYCNKLKHAFPSNVVTSLVFLNILEILHCALLEGIFE 900
                                                                        
Sbjct: 843  ------------------------------------------------------------ 902

Query: 901  VDMLTDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPHLKTIFPTSF 960
                                                                        
Sbjct: 903  ------------------------------------------------------------ 933

Query: 961  IKHMDEIENLVTDEVNEIFPEDEASKLVAPEVMHLRHYLLFTQYFVYVSICFVDFQIVFP 1020
                                                                        
Sbjct: 963  ------------------------------------------------------------ 933

Query: 1021 SLKNLKMETKRSFWFRSRSFPKLQGLVLNGFEDENMVTLPLEMSQEINLVRVKNCDKLKC 1080
                                                                        
Sbjct: 1023 ------------------------------------------------------------ 933

Query: 1081 LLPLSVSFFHLKFLTIWDCNGMINLFSSSVAKNLVSLSCIKISNCRGMSSIVATEEEEEE 1140
                                                                        
Sbjct: 1083 ------------------------------------------------------------ 933

Query: 1141 EKDEEIILKKLTDLELDDLPRLANFYSGKYSFNGTLSHDDDSELYLSHMYWINGIMIITL 1200
                                                                  IMIITL
Sbjct: 1143 ------------------------------------------------------IMIITL 933

Query: 1201 QVSFPELEDLRSEGLSLETLWHNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFL 1260
            QVSFPELEDLRSEGLSLETLWHNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFL
Sbjct: 1203 QVSFPELEDLRSEGLSLETLWHNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFL 933

Query: 1261 KILEIYTCELLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRK 1320
            KILEIYTCELLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRK
Sbjct: 1263 KILEIYTCELLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRK 933

Query: 1321 VEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPEVMHLFHYYSLAQHLYMFIFGT 1380
            VEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPE                     
Sbjct: 1323 VEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPE--------------------- 933

Query: 1381 TFEDFHDTRVIKIIVXKLLFYFQMVLSKGFGDENIVTLSVETSQESNFIQVKKCDKMNCL 1440
                                   MVLSKGFGDENIVTLSVETSQESNFIQVKKCDKMNCL
Sbjct: 1383 -----------------------MVLSKGFGDENIVTLSVETSQESNFIQVKKCDKMNCL 933

Query: 1441 LPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKD 1500
            LPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKD
Sbjct: 1443 LPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKD 933

Query: 1501 EEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI 1551
            EEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI
Sbjct: 1503 EEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI 933

BLAST of MC02g1309 vs. ExPASy TrEMBL
Match: A0A6J1DQJ3 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111022191 PE=4 SV=1)

HSP 1 Score: 1088 bits (2813), Expect = 0.0
Identity = 572/686 (83.38%), Postives = 577/686 (84.11%), Query Frame = 0

Query: 1   ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKVQILVNGRSNSYE 60
           ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTT EKVQILVN       
Sbjct: 3   ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTGEKVQILVNEE----- 62

Query: 61  IYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILEL 120
                          ++ +M    K   +R   +P                         
Sbjct: 63  ---------------EVIHMKSMTK---SRHGCWPKS----------------------- 122

Query: 121 VEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGG 180
                                                           NVNKVGVYGMGG
Sbjct: 123 ------------------------------------------------NVNKVGVYGMGG 182

Query: 181 VGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRA 240
           VGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRA
Sbjct: 183 VGKTKWLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRA 242

Query: 241 TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNM 300
           TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSR ILTNNM
Sbjct: 243 TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRHILTNNM 302

Query: 301 NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK 360
           NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK
Sbjct: 303 NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK 362

Query: 361 ELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEIC 420
           ELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEIC
Sbjct: 363 ELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEIC 422

Query: 421 AKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA 480
           AKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA
Sbjct: 423 AKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVA 482

Query: 481 KYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRI 540
           KYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRI
Sbjct: 483 KYIASNENNMSSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRI 542

Query: 541 QTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDI 600
           QTFFGEGQAIQI DTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDI
Sbjct: 543 QTFFGEGQAIQIPDTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDI 594

Query: 601 LTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEEL 660
           LTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEEL
Sbjct: 603 LTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEEL 594

Query: 661 SLWRSFKRGGEEVSYKNELIKNVKLS 686
           SLWRSFKRGGEEVSYKNELIKNVKLS
Sbjct: 663 SLWRSFKRGGEEVSYKNELIKNVKLS 594

BLAST of MC02g1309 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 912 bits (2357), Expect = 4.00e-306
Identity = 629/1569 (40.09%), Postives = 848/1569 (54.05%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKV-QILVNGRSNSY 60
            IL+SV+A     T++PIG QLGYL  YNRN K+L  QLENL+TT+  V Q +   +  SY
Sbjct: 3    ILVSVIAA----TIKPIGHQLGYLVCYNRNKKELRDQLENLETTKRDVNQRVQEAKGKSY 62

Query: 61   EIYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILE 120
             I +EVS WL    N  L      +  +SN   S PSC N  +R++LSRK +K+V  IL+
Sbjct: 63   TISEEVSKWLADVDNAIL------HDELSN---SNPSCFNLARRYQLSRKREKQVNYILQ 122

Query: 121  LVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMG 180
            L+ K   F +VGY  PLP T++  +P DYQ+LES+T L + IK+ALA P V KVGVYGM 
Sbjct: 123  LMNKRNSFVEVGYRAPLPDTENIVVPGDYQVLESKTSLAKDIKNALAKPEVKKVGVYGMA 182

Query: 181  GVGKTKWLDEVKKLVL--ENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKE 240
            GVGKT  L+EVKKLVL  E++LFDR IDV +G+ N V EIQ+QIG +LN+ L    ++KE
Sbjct: 183  GVGKTYLLNEVKKLVLKGEDRLFDRAIDVRVGRFNDVTEIQEQIGDQLNIEL---PKSKE 242

Query: 241  GRATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILT 300
            GRA+FL+N LV+M  NILILLDDLW EYDL KE+GIP     SKEGCK+LMTSRS+ ILT
Sbjct: 243  GRASFLRNNLVKMEGNILILLDDLWKEYDLLKEIGIPL----SKEGCKVLMTSRSQDILT 302

Query: 301  NNMNTEKCFKVNSLSEEESWKFFMTIVGE-FDKNRIEHMAKNVVKKCGGLPIALKIIAKA 360
            NNMNT++CF+V+SLSEEESWKFFM I+G+ FD    +++AKNV K+CGGLP+AL  IAKA
Sbjct: 303  NNMNTQECFQVSSLSEEESWKFFMAIIGDKFDTIYKKNIAKNVAKECGGLPLALDTIAKA 362

Query: 361  LKGKELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDD 420
            LKGK++  W++AL KL   + + IKGVSD++Y+ L+LSY+ ++ EET+LLFLLCSVF DD
Sbjct: 363  LKGKDMHHWEDALTKLRDSIGMDIKGVSDKVYASLRLSYEHLDGEETKLLFLLCSVFADD 422

Query: 421  HEICAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLE--SNSGDNYVQMHD 480
            ++I  KDLQMYAMGM LLN+V  WE ++N V++LV+ L SSSLLLE  S+S D YV+MH+
Sbjct: 423  YKIPIKDLQMYAMGMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHN 482

Query: 481  VVRDVAKYIASNENNMSSLSYGCGQ-SEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNL 540
            VVRDVA +IAS E NMS+ + G  + +EW ++ R GS+ AIF +C +F NLP  + FP L
Sbjct: 483  VVRDVAIHIASKEGNMSTFNIGYNKVNEWEDEYRSGSHRAIFANCDNFNNLPLKMNFPQL 542

Query: 541  QMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMS 600
            ++LILR+  +  E   +QI   FF+GM+KL+VLD+T M       + T SL NL+TLCM 
Sbjct: 543  ELLILRVSNWLVENN-LQIPYAFFDGMEKLKVLDLTGMCCLRPLWT-TPSLNNLRTLCML 602

Query: 601  YCECDDILTIFQLKTLEILRI-NGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLIS 660
             CE +DI TI +LK LE+LRI   + +  LP TM  LTQLKVL+V NCS LEV+PAN+ S
Sbjct: 603  RCEFNDIDTIGELKKLEVLRIVKCNMLDHLPPTMSQLTQLKVLEVLNCSKLEVVPANVFS 662

Query: 661  SMTKLEELSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLS 720
            SMTKLEEL L  SF R GEEV YK+ L+KNV +S LN L  LSNL LE  NV+ILS+ ++
Sbjct: 663  SMTKLEELKLQDSFCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILSE-IN 722

Query: 721  LERVEKLEEYCICVGDNEDF---EICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRV 780
             +  +KL+E+ IC  +++DF   +  +EYA +L++NI+SQI S    LEILL+  E+L V
Sbjct: 723  SQTCKKLKEFWICSNESDDFLQPKASNEYATTLMLNIESQIGSIDEGLEILLQRSERLIV 782

Query: 781  KDSVG-FINHLFKPNGNYCYPRMKNLHI---EGNSDMPRHIDFQVPAKAFRLFFFFINFT 840
             DS G FIN +FKPNG   YP +K L I    GNS+MP                      
Sbjct: 783  SDSKGNFINAIFKPNGLNGYPYLKYLWIIDENGNSEMP---------------------- 842

Query: 841  FICCFKKIYIYIYLFTTHFRNSITLLRKKQKLQTIRIQYCNKLKHAFPSNVVTSLVFLNI 900
                                                                        
Sbjct: 843  ------------------------------------------------------------ 902

Query: 901  LEILHCALLEGIFEVDMLTDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEV 960
                                        +LI      LKY+                   
Sbjct: 903  ----------------------------HLIGSDFTSLKYL------------------- 962

Query: 961  SRCPHLKTIFPTSFIKHMDEIENLVTDEVNEIFPEDEASKLVAPEVMHLRHYLLFTQYFV 1020
                          I  M  +EN+V   ++           ++P                
Sbjct: 963  -------------IIFGMKRLENIVPRHIS-----------ISP---------------- 1022

Query: 1021 YVSICFVDFQIVFPSLKNLKMETKRSFWFRSRSFPKLQGLVLNGFEDENMVTLPLEMSQE 1080
                        F S+K + ++                                      
Sbjct: 1023 ------------FKSVKTIAIQF------------------------------------- 1082

Query: 1081 INLVRVKNCDKLKCLLPLSVSFFHLKFLTIWDCNGMINLFSSSVAKNLVSLSCIKISNCR 1140
                                            C  + NLFS S+ K+L+ L  I++ NC 
Sbjct: 1083 --------------------------------CGQIRNLFSFSIFKDLLDLQEIEVINCG 1142

Query: 1141 GMSSIVATEEEEEEEKDEEIILKKLTDLELDDLPRLANFYSGKYSFNGTLSHDDDSELYL 1200
             M  IV  E E++      I    LT L+L+++ +L +F +      G +     S +  
Sbjct: 1143 NMEGIVFMEIEDQLN----ICTSPLTSLQLENVDKLTSFCT-----KGLVQESSQSII-- 1197

Query: 1201 SHMYWINGIMIITLQVSFPELEDLRSEG-LSLETLWH-NNGLSTHPFCKLQNIRIQYCNK 1260
                + +G      QVSFPEL DL   G  +LETLWH NN  +T+ FCKLQ+IRI+ C +
Sbjct: 1203 ---PFFDG------QVSFPELNDLSIVGGYNLETLWHKNNNPTTNSFCKLQSIRIEQCTQ 1197

Query: 1261 LKYVFPSNVVTSLVFLKILEIYTCELLEGIFEVEKLTDEARATV-PLTKLILISLPKLKY 1320
            L+ +FPSN+  SL  L  ++I +C  L+ IFE+E  +   +  V  L +L L+ LP LK+
Sbjct: 1263 LRCMFPSNMFASLTSLHTIQIISCASLKRIFEIENQSFSVKTVVWSLNELHLLDLPNLKH 1197

Query: 1321 VWDKDPGEFLTFPNLRKVEVSGCPRLKSFVPTSFIRHMDETKTLVTDEASKLVVPEVMHL 1380
            VW KD  + LTFP+L++V++ GC +L                                  
Sbjct: 1323 VWRKDIIKILTFPSLKRVKIHGCTKL---------------------------------- 1197

Query: 1381 FHYYSLAQHLYMFIFGTTFEDFHDTRVIKIIVXKLLFYFQMVLSKGFGDENIVTLSVETS 1440
                                    T V K                   D    T S ++ 
Sbjct: 1383 ------------------------THVWK-------------------DNTKETRSFDSL 1197

Query: 1441 QESNFIQVKKCDKMNCLLPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISN 1500
            +    I+V+KC  +  LLPSSI+F NL  L I +CNGM+NLFSS+V K LV+L  + +S 
Sbjct: 1443 ER---IEVEKCKNLKYLLPSSITFLNLKELHIKKCNGMMNLFSSTVTKKLVNLRSIKVSY 1197

Query: 1501 CEEMSSIIAREEEEEKDEEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCP 1551
            C+ M  ++  ++EE  DE I+FK+L+ LELD LPRL +F+SGK  L+FPCL +L I +CP
Sbjct: 1503 CKRMRCMVEGDQEE-NDEIIVFKRLSRLELDYLPRLDSFHSGKCMLEFPCLESLVIKRCP 1197

BLAST of MC02g1309 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 875 bits (2262), Expect = 9.52e-293
Identity = 555/1202 (46.17%), Postives = 766/1202 (63.73%), Query Frame = 0

Query: 1    ILISVVAKIAEYTVEPIGRQLGYLFFYNRNIKDLEKQLENLKTTREKV-QILVNGRSNSY 60
            IL+SV+A     T++PIG QLGYL  YNRN K+L +QLENL+TT++ V Q +   +  SY
Sbjct: 3    ILVSVIAA----TIKPIGHQLGYLVCYNRNKKELREQLENLETTKKDVNQRVEEAKGKSY 62

Query: 61   EIYDEVSTWLLAEVNVQLPNMPQQNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILE 120
             I +EVS WL A+V+  + +    N        S PSC N  +R++LSRK +K+V  IL+
Sbjct: 63   TISEEVSKWL-ADVDNAITHDELSN--------SNPSCFNLAQRYQLSRKREKQVNYILQ 122

Query: 121  LVEKGKKFDKVGYPVPLPHTKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMG 180
            L+ K   F +VGY  PLP T++  +P DYQ+LES+TLL + IK+AL+ P VNK+GVYGM 
Sbjct: 123  LMNKRNSFVEVGYRAPLPDTENTVVPGDYQVLESKTLLAKDIKNALSKPEVNKIGVYGMA 182

Query: 181  GVGKTKWLDEVKKLVL--ENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKE 240
            GVGKT +L+EVKKLVL  E++LFDRVIDV +G+ N V +IQ+QIG +LN+ L    ++KE
Sbjct: 183  GVGKTYFLNEVKKLVLKGEDRLFDRVIDVRVGRFNDVTDIQEQIGDQLNVEL---PKSKE 242

Query: 241  GRATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILT 300
            GRA+FL+N L +M  NILILLDDLW EYDL KE+GIP     SK+GCK+L+TSRS+ ILT
Sbjct: 243  GRASFLRNNLAKMEGNILILLDDLWKEYDLLKEIGIPL----SKDGCKVLITSRSQDILT 302

Query: 301  NNMNTEKCFKVNSLSEEESWKFFMTIVGE-FDKNRIEHMAKNVVKKCGGLPIALKIIAKA 360
            NNMNT++CF+V+SLSEEESWKFFM I+G+ FD    +++AKNV K+CGGLP+AL  IAKA
Sbjct: 303  NNMNTQECFQVSSLSEEESWKFFMAIIGDKFDTIYKKNIAKNVAKECGGLPLALDTIAKA 362

Query: 361  LKGKELRIWKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDD 420
            LKGK++  W++AL KL   + + IKGVSD++Y+ L+LSYD ++ EET+L+FLLCSVFPDD
Sbjct: 363  LKGKDMHHWEDALTKLRNSIGMDIKGVSDKVYASLRLSYDHLDGEETKLIFLLCSVFPDD 422

Query: 421  HEICAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLE--SNSGDNYVQMHD 480
            ++I  K+LQMYAM M LLN+V  WE ++N V++LV+ L SSSLLLE  S+S D YV+MHD
Sbjct: 423  YKISIKNLQMYAMCMRLLNKVKTWEDSKNRVMKLVNDLISSSLLLEAESDSKDKYVKMHD 482

Query: 481  VVRDVAKYIASNENNMSSLSYGCGQ-SEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNL 540
            VVRDVA +IAS E NMS+L+ G  + +EW ++ R GS+ AIF +C +  NLP  + FP L
Sbjct: 483  VVRDVAIHIASKEGNMSTLNIGYNKVNEWEDECRSGSHRAIFANCDNLNNLPLKMNFPQL 542

Query: 541  QMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMS 600
            ++LILR+  +  E   +QI   FF+GM KL+VLD+T M       + T SL NLQ LCM 
Sbjct: 543  ELLILRVSYWLVEDN-LQIPYAFFDGMVKLKVLDLTGMCCLRPLWT-TPSLNNLQALCML 602

Query: 601  YCECDDILTIFQLKTLEILRI-NGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLIS 660
             CE +DI TI +LK LE+LRI   + +  LP TM  LT LKVL+V NC  LEV+PAN+ S
Sbjct: 603  RCEFNDIDTIGELKKLEVLRIVKCNMLDHLPPTMSQLTHLKVLEVLNCPKLEVVPANIFS 662

Query: 661  SMTKLEELSLWRSFKRGGEEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLS 720
            SMTKLEEL L  SF R GEEV YK+ L+KNV +S LN L  LSNL LE  NV+ILS+ +S
Sbjct: 663  SMTKLEELKLQDSFCRWGEEVWYKDRLVKNVTVSELNCLPCLSNLSLESWNVKILSE-IS 722

Query: 721  LERVEKLEEYCICVGDNEDF---EICDEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRV 780
             +  +KL+E+ IC  +++DF   ++ +EYA +L++NI+SQ+ S    LEILL+  E+L V
Sbjct: 723  SQTCKKLKEFWICSNESDDFIQPKVSNEYATTLMLNIESQVGSIDEGLEILLQRSERLIV 782

Query: 781  KDSVG-FINHLFKPNGNYCYPRMKNLHI---EGNSDMPRHIDFQVPAKAFRLFFFFINFT 840
             DS G FIN +FKPNGN  YP +K L +    GNS+M   I     +  + + F      
Sbjct: 783  SDSKGNFINAMFKPNGN-GYPCLKYLWMIDENGNSEMAHLIGSDFTSLKYLIIF------ 842

Query: 841  FICCFKKIYIYIYLFTTHFRNSITLLRKKQKLQTIRIQYCNKLKHAFPSNVVTSLVFLNI 900
                 K++              I+L   K K++TI IQ+C ++++ F  ++   L+ L  
Sbjct: 843  ---GMKRL-------ENIVPRHISLSPFK-KVKTIAIQFCGQIRNLFSFSIFKDLLDLQE 902

Query: 901  LEILHCALLEGIFEVDMLTDEAKVV--PLKNLILISLPKLKYVWNKDP------------ 960
            +E+++C  +EGI  ++ + D+  +   PL +L L ++ KL     KD             
Sbjct: 903  IEVINCGKMEGIIFME-IGDQLNICSCPLTSLQLENVDKLTSFCTKDLIQESSQSIIPFF 962

Query: 961  DEFMTFPNLRQVEVSRCPHLKTIF-----PTSFIKHMDEIENLVTDEVNEIFPEDEASKL 1020
            D  ++FP L  + +    +L+T++     PT+ +  ++E+  L    + +++ +D    L
Sbjct: 963  DGQVSFPELNDLSIVGGNNLETLWHKNNNPTTVLWSLNELHLLNLPNLKQVWRKDIIKIL 1022

Query: 1021 VAPEVMHLRHYLLFTQYFVYVSICFVDFQIVFPSLKNLKMETKRSFWFRSRSFPKLQGLV 1080
                                           FPSLK +K+              KL  + 
Sbjct: 1023 T------------------------------FPSLKRVKIH----------GCTKLTHV- 1082

Query: 1081 LNGFEDENMVTLPLEMSQEINLVRVKNCDKLKCLLPLSVSFFHLKFLTIWDCNGMINLFS 1140
               ++D N VT   +  + I    V+ C  LK LLP S++F +LK L I  CNGMINLFS
Sbjct: 1083 ---WKDNNKVTRSFDSLERIE---VEKCKNLKYLLPSSIAFLNLKELHIKKCNGMINLFS 1112

Query: 1141 SSVAKNLVSLSCIKISNCRGMSSIVATEEEEEEEKDEEIILKKLTDLELDDLPRLANFYS 1168
            S+V K LV+LS IK+S C+GM  +V   E ++ E DE I  KKL+ LELD LPRL +FYS
Sbjct: 1143 STVTKKLVNLSSIKVSYCKGMRCMV---EVDQAENDEIITFKKLSTLELDYLPRLDSFYS 1112

BLAST of MC02g1309 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 232.6 bits (592), Expect = 2.1e-60
Identity = 254/963 (26.38%), Postives = 451/963 (46.83%), Query Frame = 0

Query: 27  YNRNIKDLEKQLENLKTTREKV-QILVNGRSNSYEIYDEVSTWL-LAEVNVQLPNMPQQN 86
           +  N + L + LE LK  + KV + L         +  ++  WL   E NV L  +  + 
Sbjct: 2   FRSNARALNRALERLKNVQTKVNEALKRSGIQEKSLERKLRIWLRKVEENVPLGELILEK 61

Query: 87  KGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILELVEKGKK-FDKVGYPVPLPHTKSPT 146
           +          SC  +     LS K  + ++ +  L E+G+    K+             
Sbjct: 62  RS---------SCAIW-----LSDKDVEILEKVKRLEEQGQDLIKKISVNKSSREIVERV 121

Query: 147 LPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGGVGKTKWLDEVKKLVLE---NKLF 206
           L   +   ++   + +K+KD L   NV K+GV+GMGGVGKT  +  +   +L+    + F
Sbjct: 122 LGPSFHPQKTALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQF 181

Query: 207 DRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILILLDD 266
             VI VT+ +   +  +Q  I  +L  G     E        +  +L++++ N L++LDD
Sbjct: 182 ALVIWVTVSKDFDLKRVQMDIAKRL--GKRFTREQMNQLGLTICERLIDLK-NFLLILDD 241

Query: 267 LWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESWKFF 326
           +W+  DL  ++GIP   E SK+  K+++TSR R  +   M T +  KV  L E+E+W+ F
Sbjct: 242 VWHPIDLD-QLGIPLALERSKDS-KVVLTSR-RLEVCQQMMTNENIKVACLQEKEAWELF 301

Query: 327 MTIVGEF-DKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK-ELRIWKNALEKLEKRVSV 386
              VGE  + + ++ +AK+V  +C GLP+A+  I + L+GK ++ +WK+ L  L++  S 
Sbjct: 302 CHNVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR--SA 361

Query: 387 KIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGLLNEVD 446
                 ++++  LKLSYD+++D   +  FL C++FP+D+ I   +L MY +  GLL+   
Sbjct: 362 PSIDTEEKIFGTLKLSYDFLQD-NMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQH 421

Query: 447 NWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASN--ENNMSSLSYG 506
           ++E   N  + LV+ LK S LL + +S D  V+MHDVVRD A +  S+  E   S +  G
Sbjct: 422 HYEDMMNEGVTLVERLKDSCLLEDGDSCDT-VKMHDVVRDFAIWFMSSQGEGFHSLVMAG 481

Query: 507 CGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQAIQIADTF 566
            G  E+ +     S   + +       LP N+    ++ L+L +Q   G     ++ + F
Sbjct: 482 RGLIEFPQDKFVSSVQRVSLMANKLERLPNNV-IEGVETLVLLLQ---GNSHVKEVPNGF 541

Query: 567 FEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYC-ECDDILTIFQLKTLEILRIN 626
            +    LR+LD++ +R  +   S +N L +L++L +  C +  ++ ++  L  L+ L ++
Sbjct: 542 LQAFPNLRILDLSGVRIRTLPDSFSN-LHSLRSLVLRNCKKLRNLPSLESLVKLQFLDLH 601

Query: 627 GSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGGEEVSY 686
            S I+ELP  ++ L+ L+ + VSN   L+ IPA  I  ++ LE L +  S    G +   
Sbjct: 602 ESAIRELPRGLEALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEE 661

Query: 687 KNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLERVEKLEEYCICVGDNEDFEIC 746
           +       +++ L HL  L+  +L++ +     D+L+ +R+ K +               
Sbjct: 662 REGQATLDEVTCLPHLQFLAIKLLDVLSFSYEFDSLT-KRLTKFQ--------------- 721

Query: 747 DEYARSLVVNIKSQIVSNGGVLEILLEGCEQLRVKD------SVGF-INHLFKPNGNYC- 806
                 L   I+S      G      EGC  L + D      S+G+ + H+   + NYC 
Sbjct: 722 -----FLFSPIRSVSPPGTG------EGC--LAISDVNVSNASIGWLLQHVTSLDLNYCE 781

Query: 807 --YPRMKNLHIEGNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIYLFTTHFR 866
                 +NL  +  S         V  KA  + +F        C  ++ ++  L      
Sbjct: 782 GLNGMFENLVTKSKSSF-------VAMKALSIHYFPSLSLASGCESQLDLFPNLEELSLD 841

Query: 867 N----SITLLR-----KKQKLQTIRIQYCNKLKHAFPSNVVT-SLVFLNILEILHCALLE 926
           N    SI  L      + QKL+ +++  C +LK  F   ++  +L  L  ++++ C  LE
Sbjct: 842 NVNLESIGELNGFLGMRLQKLKLLQVSGCRQLKRLFSDQILAGTLPNLQEIKVVSCLRLE 895

Query: 927 GIFEVDML----TDEAKVVPLKNLILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPHL 955
            +F    +      E+ +  L  + L  LP+L+ + N    + +   +L  +EV  C  L
Sbjct: 902 ELFNFSSVPVDFCAESLLPKLTVIKLKYLPQLRSLCN----DRVVLESLEHLEVESCESL 895

BLAST of MC02g1309 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 220.3 bits (560), Expect = 1.1e-56
Identity = 235/879 (26.73%), Postives = 420/879 (47.78%), Query Frame = 0

Query: 103 RHKLSRKSKKRVKDILELVEKGKKFDKV----GYPVPLPHTKSPTLPTDYQILESRTLLT 162
           R ++SRK  K + ++  L + G +F  +      P  + H   P +   +Q + S  L  
Sbjct: 97  RPRMSRKLVKILDEVKMLEKDGIEFVDMLSVESTPERVEHV--PGVSVVHQTMASNML-- 156

Query: 163 EKIKDALADPNVNKVGVYGMGGVGKTKWLDEVKKLVLE---NKLFDRVIDVTIGQSNGVV 222
            KI+D L      K+GV+GMGGVGKT  +  +   + E    + F  VI V + +     
Sbjct: 157 AKIRDGLTSEKAQKIGVWGMGGVGKTTLVRTLNNKLREEGATQPFGLVIFVIVSKEFDPR 216

Query: 223 EIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPC 282
           E+Q QI  +L++   M+ E++E  A  +   L++ R+  L++LDD+W   DL   +GIP 
Sbjct: 217 EVQKQIAERLDIDTQME-ESEEKLARRIYVGLMKERK-FLLILDDVWKPIDLDL-LGIP- 276

Query: 283 RSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESWKFFMTIVGEFDK-NRIEH 342
           R+E +K G K+++TSR   +   +M T+   +V+ L EE++W+ F    G+  + + +  
Sbjct: 277 RTEENK-GSKVILTSRFLEV-CRSMKTDLDVRVDCLLEEDAWELFCKNAGDVVRSDHVRK 336

Query: 343 MAKNVVKKCGGLPIALKIIAKALKGKE-LRIWKNALEKLEKRVSVKIKGVSDQLYSCLKL 402
           +AK V ++CGGLP+A+  +  A++GK+ +++W + L KL K V   IK + ++++  LKL
Sbjct: 337 IAKAVSQECGGLPLAIITVGTAMRGKKNVKLWNHVLSKLSKSVP-WIKSIEEKIFQPLKL 396

Query: 403 SYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDH 462
           SYD++ED + +  FLLC++FP+D+ I   ++  Y M  G + E+ + E + N  I  V+ 
Sbjct: 397 SYDFLED-KAKFCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITTVES 456

Query: 463 LKSSSLLLESNSGDNYVQMHDVVRDVAKYI--ASNENNMSSLSYGCGQSEWREKDRYGSY 522
           LK   LL + +  D  V+MHDVVRD A +I  +S +++ S +  G G  + R+     S 
Sbjct: 457 LKDYCLLEDGDRRDT-VKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLAPSL 516

Query: 523 NAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKM 582
             + +      +LP  +E   ++  +L +Q   G     ++   F +    LR+L+++  
Sbjct: 517 RRVSLMNNKLESLPDLVEEFCVKTSVLLLQ---GNFLLKEVPIGFLQAFPTLRILNLSGT 576

Query: 583 RFESSRTSLTNSLKNLQTLCMSYC-ECDDILTIFQLKTLEILRINGSTIQELPATMDDLT 642
           R +S  +     L +L +L +  C +   + ++  L  LE+L + G+ I E P  +++L 
Sbjct: 577 RIKSFPSCSLLRLFSLHSLFLRDCFKLVKLPSLETLAKLELLDLCGTHILEFPRGLEELK 636

Query: 643 QLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGGEEVSYKNELIK-NVKLSXLN 702
           + + LD+S    LE IPA ++S ++ LE L +  S  R     S + E  K    +  + 
Sbjct: 637 RFRHLDLSRTLHLESIPARVVSRLSSLETLDMTSSHYRW----SVQGETQKGQATVEEIG 696

Query: 703 HLTHLSNLMLEIPNVEILSDNLSLERVEKLEEYCICVGDNEDFEICDEYARSLVVNIKSQ 762
            L  L  L + + +   L  N     +++L+++ + VG         +  R  + ++   
Sbjct: 697 CLQRLQVLSIRLHSSPFLL-NKRNTWIKRLKKFQLVVGSRYILRTRHDKRRLTISHLNVS 756

Query: 763 IVSNGGVL----EILLEGCEQLR------VKDSVGFINHLFKPNGNYCYPRMKNLHIE-- 822
            VS G +L     + L  C+ +       V D+ GF N             +K+L IE  
Sbjct: 757 QVSIGWLLAYTTSLALNHCQGIEAMMKKLVSDNKGFKN-------------LKSLTIENV 816

Query: 823 --GNSDMPRHIDFQVPAKAFRLFFFFINFTFICCFKKIYIYIYLFTTHFRNSITLLRKKQ 882
               +     +      ++  +     N       +++++      T       L  K +
Sbjct: 817 IINTNSWVEMVSTNTSKQSSDILDLLPN------LEELHLRRVDLETFSELQTHLGLKLE 876

Query: 883 KLQTIRIQYCNKLKHAFPSNVVTSLVFLNILEILHCALLEGIFEVDMLTDEAKVVPLKNL 942
            L+ I I  C KL+         ++  L  +EI +C  L+ + E  +L  +  V  L+ L
Sbjct: 877 TLKIIEITMCRKLRTLLDKRNFLTIPNLEEIEISYCDSLQNLHEA-LLYHQPFVPNLRVL 930

Query: 943 ILISLPKLKYVWNKDPDEFMTFPNLRQVEVSRCPHLKTI 955
            L +LP L  + N        +  L QVEV  C  L  +
Sbjct: 937 KLRNLPNLVSICNWGE----VWECLEQVEVIHCNQLNCL 930

BLAST of MC02g1309 vs. TAIR 10
Match: AT4G26090.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 201.1 bits (510), Expect = 6.7e-51
Identity = 198/693 (28.57%), Postives = 331/693 (47.76%), Query Frame = 0

Query: 31  IKDLEKQLENLKTTREKVQILV-----NGRSNSYEIYDEVSTWLLAEVNVQLPNMPQQNK 90
           I DLE  + +LK  R+ + + +      GRS S    + +S   + E    L  +  + +
Sbjct: 35  ITDLETAIGDLKAIRDDLTLRIQQDGLEGRSCSNRAREWLSAVQVTETKTALLLVRFRRR 94

Query: 91  GVSNR-EASYPSCLNFFKRHKLSRKSKKRVKDILELVEKGKKFDKVGYPVPLPHTKSPTL 150
               R    Y SC      +KL +K    +K I EL E+ +     G  + +   + P  
Sbjct: 95  EQRTRMRRRYLSCFG-CADYKLCKKVSAILKSIGELRERSEAIKTDGGSIQVTCREIPI- 154

Query: 151 PTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGGVGKTKWLDEV-KKLVLENKLFDRV 210
                ++ + T++ + ++    +     +GVYG GGVGKT  +  +  +L+ +   +D +
Sbjct: 155 ---KSVVGNTTMMEQVLEFLSEEEERGIIGVYGPGGVGKTTLMQSINNELITKGHQYDVL 214

Query: 211 IDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILILLDDLWN 270
           I V + +  G   IQ  +G +L +  + K ET E RA  +   L + R   L+LLDD+W 
Sbjct: 215 IWVQMSREFGECTIQQAVGARLGLSWDEK-ETGENRALKIYRALRQKR--FLLLLDDVWE 274

Query: 271 EYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESWKFFMTI 330
           E DL+K  G+P     +K  CK++ T+RS   L NNM  E   +V  L ++ +W+ F + 
Sbjct: 275 EIDLEK-TGVPRPDRENK--CKVMFTTRS-IALCNNMGAEYKLRVEFLEKKHAWELFCSK 334

Query: 331 VGEFD---KNRIEHMAKNVVKKCGGLPIALKIIAKALKGKEL-RIWKNALEKLEKRVSVK 390
           V   D    + I  +A+ +V KCGGLP+AL  +  A+  +E    W +A E L  R   +
Sbjct: 335 VWRKDLLESSSIRRLAEIIVSKCGGLPLALITLGGAMAHRETEEEWIHASEVL-TRFPAE 394

Query: 391 IKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGLLNEVDN 450
           +KG+ + +++ LK SYD +E +  R  FL C++FP++H I  + L  Y +G G L     
Sbjct: 395 MKGM-NYVFALLKFSYDNLESDLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFLTSSHG 454

Query: 451 WETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASNENNMSSL---SYG 510
             T       L+  LK ++ LLE+      V+MH+VVR  A ++AS +     L      
Sbjct: 455 VNTIYKGYF-LIGDLK-AACLLETGDEKTQVKMHNVVRSFALWMASEQGTYKELILVEPS 514

Query: 511 CGQSEWREKDRYGSYNAIFVDCMD--FPNLPKNLEFPNLQMLILRIQTFFGEGQAIQIAD 570
            G +E  + + +    A+ +  +D     LP+ L  P L  L+L+           +I  
Sbjct: 515 MGHTEAPKAENW--RQALVISLLDNRIQTLPEKLICPKLTTLMLQ-----QNSSLKKIPT 574

Query: 571 TFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDILTIFQLKTLEILRI 630
            FF  M  LRVLD+       S TS+T                +  L+I  L  L  L +
Sbjct: 575 GFFMHMPVLRVLDL-------SFTSIT----------------EIPLSIKYLVELYHLSM 634

Query: 631 NGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGGEEVS 690
           +G+ I  LP  + +L +LK LD+     L+ IP + I  ++KLE L+L+ S+  G E  S
Sbjct: 635 SGTKISVLPQELGNLRKLKHLDLQRTQFLQTIPRDAICWLSKLEVLNLYYSY-AGWELQS 680

Query: 691 YKNELIKNVKLSXLNHLTHLSNLMLEIPNVEIL 708
           +  +  + +  + L +L +L+ L + + ++E L
Sbjct: 695 FGEDEAEELGFADLEYLENLTTLGITVLSLETL 680

BLAST of MC02g1309 vs. TAIR 10
Match: AT5G47250.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 196.4 bits (498), Expect = 1.7e-49
Identity = 191/674 (28.34%), Postives = 314/674 (46.59%), Query Frame = 0

Query: 20  QLGYLFFYNRNIKDLEKQLENLKTTREKVQILVN-GRSNSYEIYDEVSTWLLAEVNVQLP 79
           ++G +     N+  L+   + LK  +E V   VN G     +    V+TW L++V +   
Sbjct: 22  KVGNICMLKENLVLLKSAFDELKAEKEDVVNRVNAGELKGGQRLAIVATW-LSQVEIIEE 81

Query: 80  NMPQ------------QNKGVSNREASYPSCLNFFKRHKLSRKSKKRVKDILELVEKGKK 139
           N  Q            QN     R  S   C  +F    L  K  K++ ++  L   GK 
Sbjct: 82  NTKQLMDVASARDASSQNASAVRRRLSTSGC--WFSTCNLGEKVFKKLTEVKSL--SGKD 141

Query: 140 FDKVGYPVPLPHTKSPTLPTDYQILESRTL----LTEKIKDALADPNVNKVGVYGMGGVG 199
           F +V        T+ P  P     L  +T+      EK  ++L       +G++GMGGVG
Sbjct: 142 FQEV--------TEQPPPPVVEVRLCQQTVGLDTTLEKTWESLRKDENRMLGIFGMGGVG 201

Query: 200 KTKWLDEV-KKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNM-GLNMKAETKEGRA 259
           KT  L  +  K V  +  +D VI V   +   V +IQD IG +L++   N    ++  +A
Sbjct: 202 KTTLLTLINNKFVEVSDDYDVVIWVESSKDADVGKIQDAIGERLHICDNNWSTYSRGKKA 261

Query: 260 TFLQNKLVEMRENILILLDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNM 319
           + +   L +M+   ++LLDDLW +  L   +GIP   +      K++ T+RS+ +  + M
Sbjct: 262 SEISRVLRDMKPRFVLLLDDLWEDVSL-TAIGIPVLGKK----YKVVFTTRSKDV-CSVM 321

Query: 320 NTEKCFKVNSLSEEESWKFFMTIVGEFDKNRIEHMAKNVVKKCGGLPIALKIIAKALKGK 379
              +  +V  LSE ++W  F   V     N I  +AK +V KC GLP+AL++I K +  K
Sbjct: 322 RANEDIEVQCLSENDAWDLFDMKVHCDGLNEISDIAKKIVAKCCGLPLALEVIRKTMASK 381

Query: 380 ELRI-WKNALEKLEKRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEI 439
              I W+ AL+ LE   S ++KG    ++  LKLSYD+++ +  +  FL C++FP  + I
Sbjct: 382 STVIQWRRALDTLESYRS-EMKGTEKGIFQVLKLSYDYLKTKNAK-CFLYCALFPKAYYI 441

Query: 440 CAKDLQMYAMGMGLLNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDV 499
              +L  Y +G G ++E D  E  ++   E++D+L  + LLLESN     V MHD++RD+
Sbjct: 442 KQDELVEYWIGEGFIDEKDGRERAKDRGYEIIDNLVGAGLLLESN---KKVYMHDMIRDM 501

Query: 500 AKYIAS----NENNMSSLSYGCGQ----SEWREKDRYGSYNAIFVDCMDFPNLPKNLEFP 559
           A +I S     E  +     G  Q    ++W    +   +N       +  N+P + EFP
Sbjct: 502 ALWIVSEFRDGERYVVKTDAGLSQLPDVTDWTTVTKMSLFN------NEIKNIPDDPEFP 561

Query: 560 NLQMLILRIQTFFGEGQAIQIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLC 619
           +   L   +  F    + + I   FF  M  L VLD+      S    +T   K +  L 
Sbjct: 562 DQTNL---VTLFLQNNRLVDIVGKFFLVMSTLVVLDL------SWNFQITELPKGISALV 621

Query: 620 MSYCECDDILTIFQLKTLEILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLI 666
                           +L +L ++G++I+ LP  +  L++L  L++ + S+L  +   LI
Sbjct: 622 ----------------SLRLLNLSGTSIKHLPEGLGVLSKLIHLNLESTSNLRSV--GLI 638

BLAST of MC02g1309 vs. TAIR 10
Match: AT1G15890.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 191.4 bits (485), Expect = 5.3e-48
Identity = 186/715 (26.01%), Postives = 338/715 (47.27%), Query Frame = 0

Query: 23  YLFFYNRNIKDLEKQLENLKTTREK-VQILVNGRSNSYEIYDEVSTWL--LAEVNVQLPN 82
           Y+     N++ L+  ++ L+  R+  ++ +V       +   +V  WL  + +V  Q+ +
Sbjct: 28  YILKMEANLEALQNTMQELEERRDDLLRRVVIEEDKGLQRLAQVQGWLSRVKDVCSQVND 87

Query: 83  MPQQNKGVSNREASYPSC-LNFFKRHKLSRKSKKRVKDILELVEKGKKFDKVGYPVPLPH 142
           + +     + R      C  NF           K++K +  L+ KG  F+ V   +P P 
Sbjct: 88  LLKAKSIQTERLCLCGYCSKNFISGRNYGINVLKKLKHVEGLLAKG-VFEVVAEKIPAPK 147

Query: 143 TKSPTLPTDYQILESRTLLTEKIKDALADPNVNKVGVYGMGGVGKTKWLDEVKKLVLEN- 202
            +   + T   +      +  +  ++L       +G+YGMGGVGKT  L  +    LE  
Sbjct: 148 VEKKHIQTTVGL----DAMVGRAWNSLMKDERRTLGLYGMGGVGKTTLLASINNKFLEGM 207

Query: 203 KLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAETKEGRATFLQNKLVEMRENILIL 262
             FD VI V + +      IQ+QI G+L +    K  T++ +A+++ N L    +  ++L
Sbjct: 208 NGFDLVIWVVVSKDLQNEGIQEQILGRLGLHRGWKQVTEKEKASYICNIL--NVKKFVLL 267

Query: 263 LDDLWNEYDLQKEVGIPCRSESSKEGCKILMTSRSRRILTNNMNTEKCFKVNSLSEEESW 322
           LDDLW+E DL+K +G+P    + + G KI+ T+RS+ +   +M  +   KV+ L  +E+W
Sbjct: 268 LDDLWSEVDLEK-IGVP--PLTRENGSKIVFTTRSKDV-CRDMEVDGEMKVDCLPPDEAW 327

Query: 323 KFFMTIVGEF---DKNRIEHMAKNVVKKCGGLPIALKIIAKALKGKE-LRIWKNALEKLE 382
           + F   VG         I  +A+ V +KC GLP+AL +I KA+  +E ++ W++ +  L 
Sbjct: 328 ELFQKKVGPIPLQSHEDIPTLARKVAEKCCGLPLALSVIGKAMASRETVQEWQHVIHVLN 387

Query: 383 KRVSVKIKGVSDQLYSCLKLSYDWIEDEETRLLFLLCSVFPDDHEICAKDLQMYAMGMGL 442
              S +   + +++   LK SYD ++DE+ +L FL CS+FP+D+E+  ++L  Y M  G 
Sbjct: 388 SS-SHEFPSMEEKILPVLKFSYDDLKDEKVKLCFLYCSLFPEDYEVRKEELIEYWMCEGF 447

Query: 443 LNEVDNWETTRNMVIELVDHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIASN---ENNM 502
           ++  ++ +   N   +++  L  + LL++       V+MHDV+R++A +IASN   +   
Sbjct: 448 IDGNEDEDGANNKGHDIIGSLVRAHLLMDGEL-TTKVKMHDVIREMALWIASNFGKQKET 507

Query: 503 SSLSYGCGQSEWREKDRYGSYNAIFVDCMDFPNLPKNLEFPNLQMLILRIQTFFGEGQAI 562
             +  G       +   + S   + + C    N+  +   PNL  L+L+        + +
Sbjct: 508 LCVKPGVQLCHIPKDINWESLRRMSLMCNQIANISSSSNSPNLSTLLLQ------NNKLV 567

Query: 563 QIADTFFEGMKKLRVLDMTKMRFESSRTSLTNSLKNLQTLCMSYCECDDILTIFQLKTLE 622
            I+  FF  M  L VLD+       SR S  +SL                  I +L +L+
Sbjct: 568 HISCDFFRFMPALVVLDL-------SRNSSLSSLPE---------------AISKLGSLQ 627

Query: 623 ILRINGSTIQELPATMDDLTQLKVLDVSNCSDLEVIPANLISSMTKLEELSLWRSFKRGG 682
            + ++ + I+ LP +  +L +L  L++    +LE I   + +S+  L+ L L+ S     
Sbjct: 628 YINLSTTGIKWLPVSFKELKKLIHLNLEFTDELESI-VGIATSLPNLQVLKLFSS----- 687

Query: 683 EEVSYKNELIKNVKLSXLNHLTHLSNLMLEIPNVEILSDNLSLER-VEKLEEYCI 725
             V     L++ + L     L HL  L   I +  IL     ++R V  ++  C+
Sbjct: 688 -RVCIDGSLMEELLL-----LEHLKVLTATIKDALILESIQGVDRLVSSIQALCL 689