MC02g1305 (gene) Bitter gourd (Dali-11) v1

BLAST of MC02g1305 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-115
Identity = 337/1008 (33.43%), Postives = 520/1008 (51.59%), Query Frame = 0

Query: 24   QIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLVHEAEDLLDEL 83
            ++GL +G EK+ ++L       +A L D  +++L   +++ W+ KL    +E +D+LD+ 
Sbjct: 20   ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 84   VYEDLRRKVQTAKMKK-VRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYHEAGSLGLVA 143
                   K + A+ K+ V     P    F +  K+  RMK + +           L  +A
Sbjct: 80   -------KTEAARFKQAVLGRYHPRTITFCY--KVGKRMKEMME----------KLDAIA 139

Query: 144  SESAETEID------ISQLRETVSELDDFDVVGRDVEVSSIVN-QVMDATNRHVTSILPI 203
             E     +D       +  R+T   L +  V GR+ E   IV   + + +      +LPI
Sbjct: 140  EERRNFHLDERIIERQAARRQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEEVPVLPI 199

Query: 204  VGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLDNK 263
            +GMGGLGKTTLA++VF+ +RI +HF+  IWVCVS+ F   +++ AI+E++ G S    + 
Sbjct: 200  LGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDL 259

Query: 264  EVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTTRSIEVAK 323
              L  +L++ ++GKRYFLVLDDVWNE+   WD L+  L      S  SIL+TTR  ++  
Sbjct: 260  APLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLK--IGASGASILITTRLEKIGS 319

Query: 324  IMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLVKKIGGVPLVAKVLGR 383
            IM TL  Y LS LS + CW LF++ A  +    +  L    + +VKK GGVPL AK LG 
Sbjct: 320  IMGTLQLYQLSNLSQEDCWLLFKQRAFCHQTETSPKLMEIGKEIVKKCGGVPLAAKTLGG 379

Query: 384  TVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSLKQCFAYCSNFPKDFV 443
             ++F+     W  + +SEI  L Q+EN  L  L+LS   LP   L+QCFAYC+ FPKD  
Sbjct: 380  LLRFKREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLP-LDLRQCFAYCAVFPKDTK 439

Query: 444  FRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDV-VKDDRGRIIECK 503
              K+ LI +WMA  F+ L +G  N  +ED G+  +  L  R  FQ++ VK  +      K
Sbjct: 440  IEKEYLIALWMAHSFL-LSKG--NMELEDVGNEVWNELYLRSFFQEIEVKSGKTYF---K 499

Query: 504  MHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTIIYYKEMI----PHI 563
            MHDLIHD+A  + + +      SS S+  ++  K+++ +   +  +  YK+M+      +
Sbjct: 500  MHDLIHDLATSMFSASA-----SSRSI-RQINVKDDEDM---MFIVTNYKDMMSIGFSEV 559

Query: 564  IERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKKLSESIVLLYN 623
            +          F  LRVL ++++  ++LP+S+  L HLRYLD+SG  I  L + +  L N
Sbjct: 560  VSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPKRLCKLQN 619

Query: 624  LQTLRAEDTNVL--LPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTLSCFLVGFG 683
            LQTL   +   L  LPK   +L SLR+L   +   +  MPP +  L  L+TL  F+VG  
Sbjct: 620  LQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC-PLTSMPPRIGLLTCLKTLGYFVVGER 679

Query: 684  KGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDELTLSWSWPGERNNEN 743
            KG ++  L  L NL+G++ I HLERV +  EAK + L  K NL  L++SW    +R N  
Sbjct: 680  KGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSW----DRPNRY 739

Query: 744  NHKDFEVLEGLQPPKNLQTLDIRRFPGECFP---NKIFLENLVEISLCSCSNCERLPMLG 803
              ++ +VLE L+P  NL+ L+I  F G C P   N   L+N+V I +  C NC  LP  G
Sbjct: 740  ESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFG 799

Query: 804  ELPNLDSLCIIELTKVRSIGNEFYGGISDSN--QKTLFSKLKKFRVEKMRSLVQWEDSKV 863
            ELP L+SL + +     S+  E+   + DS    +  F  L+K  +    +L   +  K 
Sbjct: 800  ELPCLESLELQD----GSVEVEY---VEDSGFLTRRRFPSLRKLHIGGFCNLKGLQRMKG 859

Query: 864  ASNVTTLFPRLEVLYISDCHQLLNLPD-------DVFGSCDENGDYHLTTVT-ISDCIQL 923
            A      FP LE + ISDC   +  P        +++G  D  G   L++++ +S    L
Sbjct: 860  AEQ----FPVLEEMKISDCPMFV-FPTLSSVKKLEIWGEADAGG---LSSISNLSTLTSL 919

Query: 924  DKLPNGLKYCHSI-QYLNVMRLSCLTLKFENVDMVSSLRIMVPQKLAHRTNLE-LNIYGS 983
                N     H++   L  M  +   L + +V  + +L+  +P  LA   NL+ L+I   
Sbjct: 920  KIFSN-----HTVTSLLEEMFKNLENLIYLSVSFLENLK-ELPTSLASLNNLKCLDIRYC 961

Query: 984  IQYDRFYNRDCEELELVDELESGSQDPSSIQSLPEWLDNLTSLQTLNL 1002
               +       E L  + EL    +  + ++ LPE L +LT+L +L +
Sbjct: 980  YALESLPEEGLEGLSSLTEL--FVEHCNMLKCLPEGLQHLTTLTSLKI 961

BLAST of MC02g1305 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 6.2e-111
Identity = 324/982 (32.99%), Postives = 497/982 (50.61%), Query Frame = 0

Query: 24  QIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLVHEAEDLLDEL 83
           ++ L +G + + + L       +A L D  +++L++  +  W+ KL    +E +D+LDE 
Sbjct: 20  ELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAATYEVDDILDE- 79

Query: 84  VYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYHEAGSLGLVAS 143
            Y+    +   ++  +    + P      FR K+  RM  +   L     E  +  L   
Sbjct: 80  -YKTKATRFSQSEYGRYHPKVIP------FRHKVGKRMDQVMKKLKAIAEERKNFHL-HE 139

Query: 144 ESAETEIDISQLRETVSELDDFDVVGRDVE----VSSIVNQVMDATNRHVTSILPIVGMG 203
           +  E +   +  RET S L +  V GRD E    V  ++N V DA +    S+LPI+GMG
Sbjct: 140 KIVERQ---AVRRETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQH---LSVLPILGMG 199

Query: 204 GLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLDNKEVLL 263
           GLGKTTLA++VF+ +R+ +HF   IW+CVSE F   +++ AI+E++ G   R    E+ L
Sbjct: 200 GLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEG---RPLLGEMDL 259

Query: 264 CELKKE----MHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTTRSIEVAK 323
             L+K+    ++GKRY LVLDDVWNE+   W  L+  L      S  S+L TTR  +V  
Sbjct: 260 APLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLK--VGASGASVLTTTRLEKVGS 319

Query: 324 IMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLVKKIGGVPLVAKVLGR 383
           IM TL  Y LS LS + CW LF + A  +   +  +L    + +VKK GGVPL AK LG 
Sbjct: 320 IMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEINPNLVAIGKEIVKKSGGVPLAAKTLGG 379

Query: 384 TVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSLKQCFAYCSNFPKDFV 443
            + F+     W  + +S I  L Q+E+  L  L+LS  +LP   LKQCFAYC+ FPKD  
Sbjct: 380 ILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLP-LDLKQCFAYCAVFPKDAK 439

Query: 444 FRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDV-VKDDRGRIIECK 503
             K++LI +WMA GF+ L +G  N  +ED GD  +K L  R  FQ++ VKD +      K
Sbjct: 440 MEKEKLISLWMAHGFL-LSKG--NMELEDVGDEVWKELYLRSFFQEIEVKDGKTYF---K 499

Query: 504 MHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTIIYYKEMIPHIIERA 563
           MHDLIHD+A  + + N      SS+++           ++     ++++  + P      
Sbjct: 500 MHDLIHDLATSLFSANT-----SSSNIREINKHSYTHMMSIGFAEVVFFYTLPP------ 559

Query: 564 IHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKKLSESIVLLYNLQTL 623
               +  F  LRVL +  ++  +LP+SI  L HLRYL++ G  ++ L + +  L NLQTL
Sbjct: 560 ----LEKFISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTL 619

Query: 624 RAEDTNVL--LPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTLSCFLVGFGKGCK 683
             +    L  LPK   +L SLR+L    +  +  MPP +  L  L+TL  F+VG  KG +
Sbjct: 620 DLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQ 679

Query: 684 ITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDELTLSWSWPGERNNENNHKD 743
           +  LG L NL GS+KI HLERV + ++AK + L  K NL  L++SW+  G    E+  ++
Sbjct: 680 LGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHIYES--EE 739

Query: 744 FEVLEGLQPPKNLQTLDIRRFPGECFP---NKIFLENLVEISLCSCSNCERLPMLGELPN 803
            +VLE L+P  NL +L I  F G   P   N   L+N+V I + +  NC  LP  G+LP 
Sbjct: 740 VKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPC 799

Query: 804 LDSLCI----IELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRVEKMRSLVQWEDSKVAS 863
           L+SL +     ++  V  +  + + G     +     KL  +    ++ L++ E  +   
Sbjct: 800 LESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKKEGEE--- 859

Query: 864 NVTTLFPRLEVLYISDCHQL--------------------LNLPDDVFGSCDENGDYHLT 923
                FP LE + I +C  L                     + P+++F +       +L 
Sbjct: 860 ----QFPVLEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFPEEMFKNL-----ANLK 919

Query: 924 TVTISDCIQLDKLPNGLKYCHSIQYLNVMRLSCL-TLKFENVDMVSSLRIM--------- 956
            +TIS C  L +LP  L   ++++ L +     L +L  E ++ +SSL  +         
Sbjct: 920 YLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLK 944

BLAST of MC02g1305 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 386.3 bits (991), Expect = 1.0e-105
Identity = 326/1019 (31.99%), Postives = 499/1019 (48.97%), Query Frame = 0

Query: 13   VLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDL 72
            VL  +      ++ L +G + + + L       +A L D  +++L+   +  W+ KL   
Sbjct: 9    VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 73   VHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHY 132
             +E +D+LDE  Y+    +   ++  +    + P      FR K+  RM  +   L    
Sbjct: 69   TYEVDDILDE--YKTKATRFLQSEYGRYHPKVIP------FRHKVGKRMDQVMKKLNAIA 128

Query: 133  HEAGSLGL---VASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMD-ATNRH 192
             E     L   +    A T       RET S L +  V GRD E   IV  +++ A++  
Sbjct: 129  EERKKFHLQEKIIERQAAT-------RETGSVLTEPQVYGRDKEKDEIVKILINTASDAQ 188

Query: 193  VTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGT 252
              S+LPI+GMGGLGKTTL+++VF+ +R+ + F   IW+C+S+ F   +++ AI+E++ G 
Sbjct: 189  KLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGK 248

Query: 253  SDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTT 312
            S    +   L  +L++ ++GKRYFLVLDDVWNE+   W  L+  L      S   +L TT
Sbjct: 249  SLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLK--VGASGAFVLTTT 308

Query: 313  RSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLVKKIGGVPL 372
            R  +V  IM TL  Y LS LS + CW+LF + A  +   +  +L    + +VKK GGVPL
Sbjct: 309  RLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEEINPNLMAIGKEIVKKCGGVPL 368

Query: 373  VAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSLKQCFAYCS 432
             AK LG  ++F+     W  + +S I  L Q+E+  L  L+LS   LP   L+QCF YC+
Sbjct: 369  AAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLP-LDLRQCFVYCA 428

Query: 433  NFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDVVKDDRG 492
             FPKD    K+ LI  WMA GF+ L +G  N  +ED G+  +  L  R  FQ+ ++ + G
Sbjct: 429  VFPKDTKMAKENLIAFWMAHGFL-LSKG--NLELEDVGNEVWNELYLRSFFQE-IEVESG 488

Query: 493  RIIECKMHDLIHDIACDV--SNENKLQLDYSSNSLDGELWTKENQKLARKLRTIIYYKEM 552
            +    KMHDLIHD+A  +  +N +   +   + + DG + +             I + E 
Sbjct: 489  KTY-FKMHDLIHDLATSLFSANTSSSNIREINANYDGYMMS-------------IGFAE- 548

Query: 553  IPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV-IKKLSESI 612
               ++       +  F  LRVL + ++++ +LP+SI  L HLRYLD+SG   I+ L + +
Sbjct: 549  ---VVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRL 608

Query: 613  VLLYNLQTLRAE--DTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTLSCF 672
              L NLQTL     D+   LPK   +L SLR+L      +    PP +  L  L++LSCF
Sbjct: 609  CKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLT-STPPRIGLLTCLKSLSCF 668

Query: 673  LVGFGKGCKITXLGPLKNLK--GSLKIFHLERVGSKEEAKSSKLVEKENLDELTLSWSWP 732
            ++G  KG +   LG LKNL   GS+ I  L+RV    +AK + L  K NL  L LSW   
Sbjct: 669  VIGKRKGHQ---LGELKNLNLYGSISITKLDRVKKDTDAKEANLSAKANLHSLCLSWDLD 728

Query: 733  GERNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFP---NKIFLENLVEISLCSCSNC 792
            G+     +  D EVLE L+P  NL+ L+I  F G   P   N+  L+N+V I +  C NC
Sbjct: 729  GK-----HRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCENC 788

Query: 793  ERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRV---EKMRSL 852
              LP  GELP L+SL   EL      G+     + D+     F  L+K  +     ++ L
Sbjct: 789  SCLPPFGELPCLESL---EL----HTGSADVEYVEDNVHPGRFPSLRKLVIWDFSNLKGL 848

Query: 853  VQWEDSK---VASNVTTLF-PRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTISD 912
            ++ E  K   V   +T  + P   +  +S    L  +  D       +    LT++ ISD
Sbjct: 849  LKMEGEKQFPVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISD 908

Query: 913  CIQLDKLPNGLKYCHSIQYLNVMRLSCLTLKFENVDMVSSLRIMVPQKLAHRTNLELNIY 972
             ++   LP              M  S   LK+  +    +L+  +P  LA      LN  
Sbjct: 909  NVEATSLP------------EEMFKSLANLKYLKISFFRNLK-ELPTSLA-----SLNAL 948

Query: 973  GSIQYDRFYNRDCEELELVDE---------LESGSQDPSSIQSLPEWLDNLTSLQTLNL 1002
             S++++      C+ LE + E          E    +   ++ LPE L +LT+L TL +
Sbjct: 969  KSLKFE-----FCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTI 948

BLAST of MC02g1305 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.2e-103
Identity = 326/1022 (31.90%), Postives = 497/1022 (48.63%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            MAE  L    + +L+ +     +++ L +G EK+ E+L       +A L+D  +++L   
Sbjct: 1    MAEAFL----QVLLENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDK 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
            ++  W+ KL    +E +D+L E   E +R +      +    F  P      FR K+  R
Sbjct: 61   AIENWLQKLNSAAYEVDDILGECKNEAIRFE------QSRLGFYHP--GIINFRHKIGRR 120

Query: 121  MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
            MK I + L     E      +  E        +  RET   L +  V GRD E   IV  
Sbjct: 121  MKEIMEKLDAISEERRKFHFL--EKITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKI 180

Query: 181  VMDATN-RHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILG 240
            +++  N      + PI+GMGGLGKTTLA+++F+ ER+ KHF+  IWVCVS+ F   +++ 
Sbjct: 181  LINNVNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIK 240

Query: 241  AILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEE 300
             I+  +  +S  +++      +L++ ++GKRY LVLDDVWN++   W +L+  LT     
Sbjct: 241  TIIGNIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLT--VGA 300

Query: 301  SENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGL 360
               SIL TTR  +V  IM TL  YHLS LS      LF + A         +L    + +
Sbjct: 301  RGASILATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQKEANPNLVAIGKEI 360

Query: 361  VKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFS 420
            VKK GGVPL AK LG  ++F+     W  + ++EI +L Q+E+  L  L+LS   LP   
Sbjct: 361  VKKCGGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESSILPALRLSYHHLP-LD 420

Query: 421  LKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLF 480
            L+QCFAYC+ FPKD    K+ LI +WMA GF+ L +G  N  +ED G+  +  L  R  F
Sbjct: 421  LRQCFAYCAVFPKDTKMIKENLITLWMAHGFL-LSKG--NLELEDVGNEVWNELYLRSFF 480

Query: 481  QDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRT 540
            Q++  + +      K+HDLIHD+A  +         +S+++  G +  + N K  +   +
Sbjct: 481  QEI--EAKSGNTYFKIHDLIHDLATSL---------FSASASCGNI-REINVKDYKHTVS 540

Query: 541  IIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIK 600
            I +       ++       +  F  LRVL ++ + +++LP+SI  L HLRYLD+S    +
Sbjct: 541  IGF-----AAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSSIGDLLHLRYLDLSCNNFR 600

Query: 601  KLSESIVLLYNLQTLRAEDTNVL--LPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQL 660
             L E +  L NLQTL   +   L  LPK   +L SLRHL       +   PP +  L  L
Sbjct: 601  SLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVD-GCPLTSTPPRIGLLTCL 660

Query: 661  QTLSCFLVGFGKGCKITXLGPLKNLK--GSLKIFHLERVGSKEEAKSSKLVEKENLDELT 720
            +TL  F+VG  KG +   LG LKNL   GS+ I HLERV +  +A+++ L  K NL  L+
Sbjct: 661  KTLGFFIVGSKKGYQ---LGELKNLNLCGSISITHLERVKNDTDAEAN-LSAKANLQSLS 720

Query: 721  LSWSWPGERNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFP---NKIFLENLVEISL 780
            +SW   G    E+  K+ +VLE L+P  NL+ L+I  F G  FP   N   LE ++ + +
Sbjct: 721  MSWDNDGPNRYES--KEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRI 780

Query: 781  CSCSNCERLPMLGELPNLDSLCI------IELTKVRSIGNEFYGGISDSNQKTLFSKLKK 840
             SC NC  LP  GELP L++L +      +E  +   + + F       + +  F  LKK
Sbjct: 781  KSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRF-------STRRSFPSLKK 840

Query: 841  FRVEKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPD-------DVFGSCDEN 900
             R+   RSL       +       FP LE + I  C  L   P        +V G+ +  
Sbjct: 841  LRIWFFRSL----KGLMKEEGEEKFPMLEEMAILYC-PLFVFPTLSSVKKLEVHGNTNTR 900

Query: 901  GDYHLTTVTISDCIQLDKLPNGLKYCHSIQYLNVMRLSCLTLKFENVDMVSSLRIMVPQK 960
            G       +IS+   L  L  G  Y  +      M  S   L+F +     +L+ +    
Sbjct: 901  G-----LSSISNLSTLTSLRIGANY-RATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSL 958

Query: 961  LAHRTNLELNIYGSIQYDRFYNRDCEELELVDELESGSQDPSSIQSLPEWLDNLTSLQTL 1002
             +      L I      + F  +  E L  + +L    +    ++ LPE L +LT+L  L
Sbjct: 961  TSLNALKRLQIESCDSLESFPEQGLEGLTSLTQL--FVKYCKMLKCLPEGLQHLTALTNL 958

BLAST of MC02g1305 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.8e-103
Identity = 309/999 (30.93%), Postives = 504/999 (50.45%), Query Frame = 0

Query: 32  EKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLVHEAEDLLDELVYEDLRRK 91
           E  LE L   LL   A L D  ++++ +  V  WV++L+D+V+ AED LD++  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  V-----QTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYHEAGSLGLVASESA 151
           +      + +++++R  +S  +        +  R++ ++  L +   +   LGL      
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGL----KE 155

Query: 152 ETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMDATNR-HVTSILPIVGMGGLGKTT 211
            T +   Q   T S +D+ +V GRD +   I+  ++    + +  +++ IVG+GG+GKTT
Sbjct: 156 LTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTT 215

Query: 212 LAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLDNKEVLLCELKKE 271
           L++L+++ + +R +F   +W  VSE F V KI   + E++        + +VL  +LK+ 
Sbjct: 216 LSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKER 275

Query: 272 MHGK--RYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTTRSIEVAKIMETLPSY 331
           + G    + LVLDD+WNEN + WD L+       + S+  ILVTTRS  VA IM  +  +
Sbjct: 276 LTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQ--ILVTTRSQRVASIMCAVHVH 335

Query: 332 HLSKLSDDQCWYLFQESANANGLP-MTSSLKVFQEGLVKKIGGVPLVAKVLGRTVKFQGN 391
           +L  LSD  CW LF ++   N  P +   +    E +V K  G+PL  K LG  ++F+G 
Sbjct: 336 NLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGK 395

Query: 392 FDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSLKQCFAYCSNFPKDFVFRKDQLI 451
              W  +L S I  L  +++  L +L++S   LP+  LK+CFAYCS FPK   F KD+++
Sbjct: 396 VIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPA-HLKRCFAYCSIFPKGHAFEKDKVV 455

Query: 452 QMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDVVKDDRGRIIECKMHDLIHDI 511
            +WMA+GF  LQ+ RS+K +E+ G+ YF  L SR L Q      + R I   MHD I+++
Sbjct: 456 LLWMAEGF--LQQTRSSKNLEELGNEYFSELESRSLLQKT----KTRYI---MHDFINEL 515

Query: 512 ACDVSNENKLQLDYSSNSLDG-ELWTKENQKLA-------------RKLRTIIYYKEMIP 571
           A   S E      +SS   DG +L   E  +                 LR + + +  +P
Sbjct: 516 AQFASGE------FSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLP 575

Query: 572 ---------HIIERAIHDKMM-NFFCLRVLIMNSNSIKELPNSIHK-LKHLRYLDISGCV 631
                      +++ + +K++     LRVL ++   I  LP    K + H R+LD+S   
Sbjct: 576 LSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTE 635

Query: 632 IKKLSESIVLLYNLQTLRAEDTNVL--LPKNLRELISLRHLEFSYTYVVEQMPPYLSQLI 691
           ++KL +S+  +YNLQTL     + L  LP ++  LI+LR+L+   T  + QMP    +L 
Sbjct: 636 LEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGT-KLRQMPRRFGRLK 695

Query: 692 QLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDELT 751
            LQTL+ F V    G +I+ LG L +L G LKI  L+RV    +A  + L  K++L E+ 
Sbjct: 696 SLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREID 755

Query: 752 LSW--SWPGERNNENNHK---DFEVLEGLQPPKNLQTLDIRRFPGECFPNKI---FLENL 811
             W        NN N H+   + EV E L+P ++++ L I R+ G  FP+ +       +
Sbjct: 756 FVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRI 815

Query: 812 VEISLCSCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGG---ISDSNQKTLFSKL 871
           V I L  C  C  LP LG+LP L  L I  +  ++SIG +FY     + D +Q+  F  L
Sbjct: 816 VCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQP-FRSL 875

Query: 872 KKFRVEKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHL 931
           +  R + +    +W D +V      LFP L+ L+I  C +L         S        L
Sbjct: 876 ETLRFDNLPDWQEWLDVRVTRG--DLFPSLKKLFILRCPELTGTLPTFLPS--------L 935

Query: 932 TTVTISDCIQLDKLPNGLKYCH-SIQYLNVMRLSCLTLKFENVDMVSSLRIMVPQKLAHR 980
            ++ I  C  LD  P+  +Y + ++Q L++ + SC TL    ++  ++L  +   +    
Sbjct: 936 ISLHIYKCGLLDFQPDHHEYSYRNLQTLSI-KSSCDTLVKFPLNHFANLDKLEVDQCTSL 995

BLAST of MC02g1305 vs. NCBI nr
Match: XP_022150842.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 1329 bits (3439), Expect = 0.0
Identity = 674/674 (100.00%), Postives = 674/674 (100.00%), Query Frame = 0

Query: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
           MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD
Sbjct: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60

Query: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
           SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR
Sbjct: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120

Query: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
           MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ
Sbjct: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180

Query: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240
           VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA
Sbjct: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300
           ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES
Sbjct: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300

Query: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360
           ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV
Sbjct: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360

Query: 361 KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSL 420
           KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSL
Sbjct: 361 KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSL 420

Query: 421 KQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQ 480
           KQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQ
Sbjct: 421 KQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQ 480

Query: 481 DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTI 540
           DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTI
Sbjct: 481 DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTI 540

Query: 541 IYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKK 600
           IYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKK
Sbjct: 541 IYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKK 600

Query: 601 LSESIVLLYNLQTLRAEDTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTL 660
           LSESIVLLYNLQTLRAEDTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTL
Sbjct: 601 LSESIVLLYNLQTLRAEDTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTL 660

Query: 661 SCFLVGFGKGCKIT 674
           SCFLVGFGKGCKIT
Sbjct: 661 SCFLVGFGKGCKIT 674

BLAST of MC02g1305 vs. NCBI nr
Match: XP_022158717.1 (putative disease resistance protein RGA3 [Momordica charantia])

HSP 1 Score: 1204 bits (3115), Expect = 0.0
Identity = 640/886 (72.23%), Postives = 730/886 (82.39%), Query Frame = 0

Query: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
           MAEFL TFA +EVLKKV KVAAEQIGLAWGL+K+L ELREWLLKTE FLRDINKRKLH D
Sbjct: 1   MAEFLWTFAVQEVLKKVAKVAAEQIGLAWGLKKELHELREWLLKTETFLRDINKRKLHHD 60

Query: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
           SVRLWVDKLQ LVHEAE LLDELVYEDLRRKV T KMKKVR+F+SPSNN   FRVKMA +
Sbjct: 61  SVRLWVDKLQHLVHEAEHLLDELVYEDLRRKVVTGKMKKVRDFVSPSNNILAFRVKMARK 120

Query: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
           MKNI+DLLCKHY EAG LGLV   SAE EIDI+QLRET+SELDDF+VVGRDVEVS IVNQ
Sbjct: 121 MKNITDLLCKHYCEAGPLGLVGDGSAEIEIDIAQLRETISELDDFEVVGRDVEVSKIVNQ 180

Query: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240
           ++DATN HVTSILPIVGMGGLGKTT+AK+VF+HERI+ HFDKTIWVCVSEPFLVNKILGA
Sbjct: 181 LIDATNTHVTSILPIVGMGGLGKTTMAKVVFNHERIKTHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300
           IL TLRGTS  LDNKE LL ELKKEMHGKRYFLVLDDVWNE+ SLWDELKNCL RITE S
Sbjct: 241 ILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESHSLWDELKNCLMRITERS 300

Query: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360
           ENSILVTTRS EVAKIMETLP++HLSKLSDDQCW LF ESAN NGL +TSSLKV QE LV
Sbjct: 301 ENSILVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVVQE-LV 360

Query: 361 KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQ--EENFALSILKLSVDRLPSF 420
           KKIGG+PLVA+VLGR VKF+G+++ WVT LES I T  Q  EE+F LSILKLSVDRLP  
Sbjct: 361 KKIGGIPLVARVLGRAVKFEGDYETWVTTLESIIRTRLQLQEESFVLSILKLSVDRLPLS 420

Query: 421 SLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCL 480
           SLKQCF+YCSNFPKDFVF K+ LI+MWMAQGFI LQE RSNKT+EDEGD YFKILLSRCL
Sbjct: 421 SLKQCFSYCSNFPKDFVFEKEPLIRMWMAQGFIQLQERRSNKTMEDEGDTYFKILLSRCL 480

Query: 481 FQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKE-NQKLARKL 540
           FQD++KD +GRII  KMHDLIHDIAC++SN+ KL LD   N LD E W  E ++K+A KL
Sbjct: 481 FQDIIKDGKGRIISFKMHDLIHDIACNISNDKKLHLD-PCNLLDNEHWRNEISEKVASKL 540

Query: 541 RTIIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV 600
           RT+I  +E + H  E+ I DKM NF  LRVLI+N  + K+LP+SI KLKHLRYLDISGC 
Sbjct: 541 RTVICRRETL-HRREQVIDDKMSNFIWLRVLIINFWNAKKLPDSIGKLKHLRYLDISGCS 600

Query: 601 IKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEFSYTYVVE---QMPPYLSQ 660
           + +L ESIV LY+LQTLR E T ++ LPKNL +L+SLRHLEFS+   ++   QMP +LSQ
Sbjct: 601 MDELPESIVSLYHLQTLRIEGTMIVDLPKNLGKLVSLRHLEFSFFLGIKKILQMPSHLSQ 660

Query: 661 LIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDE 720
           L+QLQTLS F+VGF KG KIT LGPLKNLKGSL + +LER+ SKEEA  +KL EKENL E
Sbjct: 661 LVQLQTLSTFIVGFEKGRKITELGPLKNLKGSLNLSNLERIKSKEEAMFAKLGEKENLHE 720

Query: 721 LTLSWSWPGERNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVEISLC 780
           LT +W+   ER   NNH D +VLEGLQP K+LQ+L I+ F GEC PNKIF+ENLV ISL 
Sbjct: 721 LTFTWTCSSEREY-NNHNDLDVLEGLQPHKSLQSLSIQSFLGECLPNKIFVENLVAISLV 780

Query: 781 SCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRVEKMR 840
            C NCERLPMLG+L NL+ LCI  L  VRSIGNEFYG  +DS+Q+T F KLKKF + KM 
Sbjct: 781 DCENCERLPMLGQLTNLEELCISGLHSVRSIGNEFYG--NDSDQRTFFPKLKKFTLNKMS 840

Query: 841 SLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDEN 879
           +L QWE+  +A N+T +FP +E+  IS+C +LL +P  +FGS D+N
Sbjct: 841 NLEQWEEVTLAPNLT-VFPCIEMWKISECFKLLKVPK-IFGSWDKN 878

BLAST of MC02g1305 vs. NCBI nr
Match: XP_022944234.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 606/1016 (59.65%), Postives = 751/1016 (73.92%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            M EFL TFA +E+LKKVV++AA++IGL WGLEK+L  L+EWLLK+E FL DIN RKLH+D
Sbjct: 1    MGEFLWTFAVQEMLKKVVRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHND 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQ-TAKMKKVRNFLSPSNNAFVFRVKMAN 120
            SVRLWV+KL  LVHEA+DL+D LVYE LR KVQ   KM K                    
Sbjct: 61   SVRLWVEKLHHLVHEADDLIDNLVYEHLRHKVQKNDKMNK-------------------- 120

Query: 121  RMKNISDLLCKHYHEAGSLGLVASESAET--EIDISQLRETVSELDDFDVVGRDVEVSSI 180
                    L KH  EA  LGLV  E  +T  + D+SQ+RETVSE+DDF+VVGRDVEVSSI
Sbjct: 121  --------LNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSI 180

Query: 181  VNQVMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKI 240
            V QV+DA N+ VTSILPIVGMGGLGKTTLA+L+F+HE I++HFD+TIWVCVS PF +NKI
Sbjct: 181  VKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKI 240

Query: 241  LGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRIT 300
            LGAIL+ ++G      N+E+LL ELKKEM+GKRYFLVLDDVWNE+  LW++LKN L +IT
Sbjct: 241  LGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKIT 300

Query: 301  EESENSILVTTRSIEVAKIME-TLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQ 360
             +S NSILVTTR++EV KIME  LP++H++KLSD+QCW LF ESANANGLP TS+L+  +
Sbjct: 301  SKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVR 360

Query: 361  EGLVKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLF--QEENFALSILKLSVDR 420
            E LVKK GG+PLVA+VLG  VKF+ ++DIWV++LE  + +    Q +NF L ILKLSVD 
Sbjct: 361  EELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDY 420

Query: 421  LPSFSLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRS-NKTIEDEGDRYFKIL 480
            LPS SLK CFAYCSNFPKD+ FRKD LIQMW+AQGFI LQEGRS  +T+E+EG+RYFKIL
Sbjct: 421  LPS-SLKPCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKIL 480

Query: 481  LSRCLFQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKL 540
            LSRCLFQDVVKD RGRII CKMHDLIHD+ACDVSN+ K  LD S  S D ELW    + +
Sbjct: 481  LSRCLFQDVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLD-SCTSSDEELWRNRTKNI 540

Query: 541  ARKLRTIIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDI 600
              KLR I  ++ M PH IE+A+HDK+ +F CLRVLIMNS SI +LP SI +LKHLRY+DI
Sbjct: 541  GSKLRMIACHEGM-PHRIEKAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDI 600

Query: 601  SGCVIKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEFSYTYVVE--QMPPY 660
            SGC+I+KL +SIVLLYNL+TLR   + +  LPK+LR L++LRHLEFS  Y V+  +MPP+
Sbjct: 601  SGCLIQKLPKSIVLLYNLKTLRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPH 660

Query: 661  LSQLIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKEN 720
            LSQLI LQTLSCF+VGF K C+I  LGPLKNLKGSLKI +LERV S+EEAKS+  +EKE 
Sbjct: 661  LSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEK 720

Query: 721  LDELTLSWSWPGE-RNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVE 780
            L EL L W W  E  ++ +NH  ++VLEGLQP K L +L I  F GEC  N IF++NLV 
Sbjct: 721  LRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVA 780

Query: 781  ISLCSCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRV 840
            ISL  C NC RLPMLG L +L+ L +  L  VRSIG+EFYG  + SNQ+ LF K+K F++
Sbjct: 781  ISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYG--NHSNQRALFPKVKIFKL 840

Query: 841  EKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTI 900
             ++  L QWE+  V ++VTT FP+L+VL IS C +LL++  D+FG CDENG  HL  + I
Sbjct: 841  IEVERLEQWEEVTVGAHVTT-FPQLQVLEISKCPRLLSI-SDLFGCCDENGVQHLRVLQI 900

Query: 901  SDCIQLDKLPNGLKYCHSIQYLNVM-RLSCLTLKFENVDMVSSLRIMVPQKLAHRTNL-E 960
             +C +L KLPNGL++CHSIQ+L ++   S L L  +N  ++  L    P++LAH + L E
Sbjct: 901  LECSKLTKLPNGLQFCHSIQHLRLIDNCSFLALDLQNEHLLMRL----PERLAHLSELKE 960

Query: 961  LNIYGSIQYDRFYN-RDCEE-LELVDELESGSQDPSSIQSLPEWLDNLTSLQTLNL 1001
            ++I G  Q+    N R     ++L+   E   QD S IQSLPEWL+N TSLQ LNL
Sbjct: 961  MDIVGCTQHGDLMNLRPVRPVIKLLSNDEHRFQDLSEIQSLPEWLENFTSLQLLNL 977

BLAST of MC02g1305 vs. NCBI nr
Match: XP_022148515.1 (disease resistance protein RGA2-like [Momordica charantia])

HSP 1 Score: 1085 bits (2806), Expect = 0.0
Identity = 607/1032 (58.82%), Postives = 742/1032 (71.90%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            MAE L T   E+VL+K+  V AEQIG+A G    LEEL + L++ E  +    K+  H D
Sbjct: 1    MAESLWTCVVEKVLEKIASVVAEQIGVATGFNNALEELHKNLVEAENIINATTKKTSHDD 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
             +R  V+ LQ  V EA D+LD +VYEDLR+KV++    KV      S  +  FR+K A  
Sbjct: 61   LLRKGVNDLQLTVDEANDVLDLIVYEDLRKKVKSQTKWKVPFSFCTSKYSPAFRLKRAKE 120

Query: 121  MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
            +K I  +L +H  +   L  +   + ETEID++QLRET S++DDFD+VGRDVE+SSIV++
Sbjct: 121  IKEILGMLSEHMDKLPKLASLGDNTTETEIDMNQLRETDSKIDDFDIVGRDVELSSIVDR 180

Query: 181  VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDK-TIWVCVSEPFLVNKILG 240
            V+DATNRHVTSILPIVG+GGLGKTTLAKLV +HERI+ HF+  TIWVCVS+PF VNKILG
Sbjct: 181  VIDATNRHVTSILPIVGVGGLGKTTLAKLVINHERIKHHFNNNTIWVCVSQPFNVNKILG 240

Query: 241  AILETLRGTSDRLD-NKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITE 300
             ILETL+ T   L+  KEVLL +L KEM GK+YFLVLDDVWNEN  LWDELKNCL +ITE
Sbjct: 241  TILETLKDTPSGLEYKKEVLLGQLYKEMRGKKYFLVLDDVWNENRLLWDELKNCLMKITE 300

Query: 301  ESENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEG 360
             SEN ILVTTRS EVAKIMETLPS+HL KLSDDQCW LFQESANANGL MTSSLKV +E 
Sbjct: 301  NSENGILVTTRSAEVAKIMETLPSHHLGKLSDDQCWSLFQESANANGLSMTSSLKVVREE 360

Query: 361  LVKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSF 420
            LVKKIGG+PL+AKV G   ++ G+ D WV  L+S +    Q EN A S LKLSVDRLP  
Sbjct: 361  LVKKIGGLPLIAKVWGSAEQYLGDPDKWVINLKSLVCNSLQNENSATSALKLSVDRLPRH 420

Query: 421  SLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCL 480
            SLKQCFAYCSNFPKDF F K+QLIQMW+AQGFIHL +GRSN+TIEDEGDR+FKILL RCL
Sbjct: 421  SLKQCFAYCSNFPKDFEFTKEQLIQMWIAQGFIHLPKGRSNETIEDEGDRHFKILLRRCL 480

Query: 481  FQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLR 540
            FQDVV+DDRG I+ CKMHDLIHDIACDVSN++KLQ  YS+NSLDGE+WT EN+K+A KLR
Sbjct: 481  FQDVVQDDRGIIVSCKMHDLIHDIACDVSNDHKLQ--YSNNSLDGEVWTDENKKIASKLR 540

Query: 541  TIIYYKE-MIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV 600
            TIIY ++ M+P I+E+ IHDK+MNF CLRVL MNS SI ELP+SI +LKHLRYLDISGC 
Sbjct: 541  TIIYCEDQMMPPIMEKVIHDKVMNFVCLRVLKMNSRSISELPDSIGELKHLRYLDISGCS 600

Query: 601  IKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEF-SYTYVVEQMPPYLSQLI 660
            I +L +SI LLYNLQTL      +  LPKN+ +L++L+HL+F  Y +   +MP Y  +L 
Sbjct: 601  ITQLPKSIALLYNLQTLNMGGLLIKDLPKNIIDLVNLKHLQFYPYHFDTYKMPAYFGKLT 660

Query: 661  QLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDELT 720
            QLQTLS F+VGF  G KI+ LG LKNLKGSL +  LE+V SKEEA+++KLVEKENLDEL 
Sbjct: 661  QLQTLSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKEEARAAKLVEKENLDELE 720

Query: 721  LSWSWPGERNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVEISLCSC 780
              WS    R + +++KDFE+LEGLQP KNL++L I R   +  PN IF+ENL  ISL  C
Sbjct: 721  FEWSQSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSLPNNIFVENLAVISLRDC 780

Query: 781  SNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRVEKMRSL 840
             NCERLPMLG+L NL+ L +  L  V+SI NEFYG  +D NQ+TLF KL+ F +  M +L
Sbjct: 781  QNCERLPMLGQLRNLERLEVYGLHSVQSISNEFYG--NDCNQRTLFPKLRAFLLSDMENL 840

Query: 841  VQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTISDCIQL 900
             QW++  VASN+TT FP LE L I  C +L+N+PD VFG CDENG  HL  VTIS C  L
Sbjct: 841  EQWKEVTVASNLTT-FPCLESLNIWRCPKLMNIPD-VFGYCDENGVEHLRHVTISGCDGL 900

Query: 901  DKLPNGLKYCHSIQYLNVMRLSCLTLKFENVDMVSSLRI----MVPQKLAHRTNLE-LNI 960
            +KLPNGL++CHSI+ L +   S LTL  +N + +SSL I     +P+ +AH   LE + I
Sbjct: 901  NKLPNGLQFCHSIEDLTIEDCSNLTLSLQNKNRLSSLSIGGLHKLPEGMAHLKKLEAMKI 960

Query: 961  YGSIQ-YD---RFYNRDCEELELVDELESGSQDPSS-----------------IQSLPEW 1001
             G IQ YD     +      L L D+L + +  P                   I++LPEW
Sbjct: 961  NGGIQDYDFSPLMHLPSLTYLHLCDDLGTATHVPRQVEHCTTLKFLIIENFEHIEALPEW 1020

BLAST of MC02g1305 vs. NCBI nr
Match: XP_022944235.1 (disease resistance protein RGA2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 591/1016 (58.17%), Postives = 724/1016 (71.26%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            M EFL TFA +E+LKKVV++AA++IGL WGLEK+L  L+EWLLK+E FL DIN RKLH+D
Sbjct: 1    MGEFLWTFAVQEMLKKVVRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHND 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQ-TAKMKKVRNFLSPSNNAFVFRVKMAN 120
            SVRLWV+KL  LVHEA+DL+D LVYE LR KVQ   KM KV NFL PSNN+ VFR KMA 
Sbjct: 61   SVRLWVEKLHHLVHEADDLIDNLVYEHLRHKVQKNDKMNKVHNFLFPSNNSLVFRYKMAK 120

Query: 121  RMKNISDLLCKHYHEAGSLGLVASESAET--EIDISQLRETVSELDDFDVVGRDVEVSSI 180
            +M N  + L KH  EA  LGLV  E  +T  + D+SQ+RETVSE+DDF+VVGRDVEVSSI
Sbjct: 121  KMNNFMNQLNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSI 180

Query: 181  VNQVMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKI 240
            V QV+DA N+ VTSILPIVGMGGLGKTTLA+L+F+HE I++HFD+TIWVCVS PF +NKI
Sbjct: 181  VKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKI 240

Query: 241  LGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRIT 300
            LGAIL+ ++G      N+E+LL ELKKEM+GKRYFLVLDDVWNE+  LW++LKN L +IT
Sbjct: 241  LGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKIT 300

Query: 301  EESENSILVTTRSIEVAKIME-TLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQ 360
             +S NSILVTTR++EV KIME  LP++H++KLSD+QCW LF ESANANGLP TS+L+  +
Sbjct: 301  SKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVR 360

Query: 361  EGLVKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLF--QEENFALSILKLSVDR 420
            E LVKK GG+PLVA+VLG  VKF+ ++DIWV++LE  + +    Q +NF L ILKLSVD 
Sbjct: 361  EELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDY 420

Query: 421  LPSFSLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRS-NKTIEDEGDRYFKIL 480
            LPS SLK CFAYCSNFPKD+ FRKD LIQMW+AQGFI LQEGRS  +T+E+EG+RYFKIL
Sbjct: 421  LPS-SLKPCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKIL 480

Query: 481  LSRCLFQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKL 540
            LSRCLFQDVVKD RGRII CKMHDLIHD+ACDVSN+ K  LD S  S D ELW    + +
Sbjct: 481  LSRCLFQDVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLD-SCTSSDEELWRNRTKNI 540

Query: 541  ARKLRTIIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDI 600
              KLR I  ++ M PH IE+A+HDK+ +F CLRVLIMNS SI +LP SI +LKHLRY+DI
Sbjct: 541  GSKLRMIACHEGM-PHRIEKAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDI 600

Query: 601  SGCVIKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEFSYTYVVE--QMPPY 660
            SGC+I+KL +SIVLLYNL+TLR   + +  LPK+LR L++LRHLEFS  Y V+  +MPP+
Sbjct: 601  SGCLIQKLPKSIVLLYNLKTLRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPH 660

Query: 661  LSQLIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKEN 720
            LSQLI LQTLSCF+VGF K C+I  LGPLKNLKGSLKI +LERV S+EEAKS+  +EKE 
Sbjct: 661  LSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEK 720

Query: 721  LDELTLSWSWPGE-RNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVE 780
            L EL L W W  E  ++ +NH  ++VLEGLQP K L +L I  F GEC  N IF++NLV 
Sbjct: 721  LRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVA 780

Query: 781  ISLCSCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRV 840
            ISL  C NC RLPMLG L +L+ L +  L  VRSIG+EFYG  + SNQ            
Sbjct: 781  ISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYG--NHSNQ------------ 840

Query: 841  EKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTI 900
                                                                 HL  + I
Sbjct: 841  -----------------------------------------------------HLRVLQI 900

Query: 901  SDCIQLDKLPNGLKYCHSIQYLNVM-RLSCLTLKFENVDMVSSLRIMVPQKLAHRTNL-E 960
             +C +L KLPNGL++CHSIQ+L ++   S L L  +N  ++  L    P++LAH + L E
Sbjct: 901  LECSKLTKLPNGLQFCHSIQHLRLIDNCSFLALDLQNEHLLMRL----PERLAHLSELKE 942

Query: 961  LNIYGSIQYDRFYN-RDCEE-LELVDELESGSQDPSSIQSLPEWLDNLTSLQTLNL 1001
            ++I G  Q+    N R     ++L+   E   QD S IQSLPEWL+N TSLQ LNL
Sbjct: 961  MDIVGCTQHGDLMNLRPVRPVIKLLSNDEHRFQDLSEIQSLPEWLENFTSLQLLNL 942

BLAST of MC02g1305 vs. ExPASy TrEMBL
Match: A0A6J1DBV6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111018890 PE=4 SV=1)

HSP 1 Score: 1329 bits (3439), Expect = 0.0
Identity = 674/674 (100.00%), Postives = 674/674 (100.00%), Query Frame = 0

Query: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
           MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD
Sbjct: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60

Query: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
           SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR
Sbjct: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120

Query: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
           MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ
Sbjct: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180

Query: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240
           VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA
Sbjct: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300
           ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES
Sbjct: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300

Query: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360
           ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV
Sbjct: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360

Query: 361 KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSL 420
           KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSL
Sbjct: 361 KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSL 420

Query: 421 KQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQ 480
           KQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQ
Sbjct: 421 KQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQ 480

Query: 481 DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTI 540
           DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTI
Sbjct: 481 DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLRTI 540

Query: 541 IYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKK 600
           IYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKK
Sbjct: 541 IYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCVIKK 600

Query: 601 LSESIVLLYNLQTLRAEDTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTL 660
           LSESIVLLYNLQTLRAEDTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTL
Sbjct: 601 LSESIVLLYNLQTLRAEDTNVLLPKNLRELISLRHLEFSYTYVVEQMPPYLSQLIQLQTL 660

Query: 661 SCFLVGFGKGCKIT 674
           SCFLVGFGKGCKIT
Sbjct: 661 SCFLVGFGKGCKIT 674

BLAST of MC02g1305 vs. ExPASy TrEMBL
Match: A0A6J1DXY1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111025175 PE=4 SV=1)

HSP 1 Score: 1204 bits (3115), Expect = 0.0
Identity = 640/886 (72.23%), Postives = 730/886 (82.39%), Query Frame = 0

Query: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
           MAEFL TFA +EVLKKV KVAAEQIGLAWGL+K+L ELREWLLKTE FLRDINKRKLH D
Sbjct: 1   MAEFLWTFAVQEVLKKVAKVAAEQIGLAWGLKKELHELREWLLKTETFLRDINKRKLHHD 60

Query: 61  SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
           SVRLWVDKLQ LVHEAE LLDELVYEDLRRKV T KMKKVR+F+SPSNN   FRVKMA +
Sbjct: 61  SVRLWVDKLQHLVHEAEHLLDELVYEDLRRKVVTGKMKKVRDFVSPSNNILAFRVKMARK 120

Query: 121 MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
           MKNI+DLLCKHY EAG LGLV   SAE EIDI+QLRET+SELDDF+VVGRDVEVS IVNQ
Sbjct: 121 MKNITDLLCKHYCEAGPLGLVGDGSAEIEIDIAQLRETISELDDFEVVGRDVEVSKIVNQ 180

Query: 181 VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGA 240
           ++DATN HVTSILPIVGMGGLGKTT+AK+VF+HERI+ HFDKTIWVCVSEPFLVNKILGA
Sbjct: 181 LIDATNTHVTSILPIVGMGGLGKTTMAKVVFNHERIKTHFDKTIWVCVSEPFLVNKILGA 240

Query: 241 ILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEES 300
           IL TLRGTS  LDNKE LL ELKKEMHGKRYFLVLDDVWNE+ SLWDELKNCL RITE S
Sbjct: 241 ILGTLRGTSSGLDNKEALLHELKKEMHGKRYFLVLDDVWNESHSLWDELKNCLMRITERS 300

Query: 301 ENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEGLV 360
           ENSILVTTRS EVAKIMETLP++HLSKLSDDQCW LF ESAN NGL +TSSLKV QE LV
Sbjct: 301 ENSILVTTRSAEVAKIMETLPNHHLSKLSDDQCWSLFLESANVNGLSITSSLKVVQE-LV 360

Query: 361 KKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQ--EENFALSILKLSVDRLPSF 420
           KKIGG+PLVA+VLGR VKF+G+++ WVT LES I T  Q  EE+F LSILKLSVDRLP  
Sbjct: 361 KKIGGIPLVARVLGRAVKFEGDYETWVTTLESIIRTRLQLQEESFVLSILKLSVDRLPLS 420

Query: 421 SLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCL 480
           SLKQCF+YCSNFPKDFVF K+ LI+MWMAQGFI LQE RSNKT+EDEGD YFKILLSRCL
Sbjct: 421 SLKQCFSYCSNFPKDFVFEKEPLIRMWMAQGFIQLQERRSNKTMEDEGDTYFKILLSRCL 480

Query: 481 FQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKE-NQKLARKL 540
           FQD++KD +GRII  KMHDLIHDIAC++SN+ KL LD   N LD E W  E ++K+A KL
Sbjct: 481 FQDIIKDGKGRIISFKMHDLIHDIACNISNDKKLHLD-PCNLLDNEHWRNEISEKVASKL 540

Query: 541 RTIIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV 600
           RT+I  +E + H  E+ I DKM NF  LRVLI+N  + K+LP+SI KLKHLRYLDISGC 
Sbjct: 541 RTVICRRETL-HRREQVIDDKMSNFIWLRVLIINFWNAKKLPDSIGKLKHLRYLDISGCS 600

Query: 601 IKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEFSYTYVVE---QMPPYLSQ 660
           + +L ESIV LY+LQTLR E T ++ LPKNL +L+SLRHLEFS+   ++   QMP +LSQ
Sbjct: 601 MDELPESIVSLYHLQTLRIEGTMIVDLPKNLGKLVSLRHLEFSFFLGIKKILQMPSHLSQ 660

Query: 661 LIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDE 720
           L+QLQTLS F+VGF KG KIT LGPLKNLKGSL + +LER+ SKEEA  +KL EKENL E
Sbjct: 661 LVQLQTLSTFIVGFEKGRKITELGPLKNLKGSLNLSNLERIKSKEEAMFAKLGEKENLHE 720

Query: 721 LTLSWSWPGERNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVEISLC 780
           LT +W+   ER   NNH D +VLEGLQP K+LQ+L I+ F GEC PNKIF+ENLV ISL 
Sbjct: 721 LTFTWTCSSEREY-NNHNDLDVLEGLQPHKSLQSLSIQSFLGECLPNKIFVENLVAISLV 780

Query: 781 SCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRVEKMR 840
            C NCERLPMLG+L NL+ LCI  L  VRSIGNEFYG  +DS+Q+T F KLKKF + KM 
Sbjct: 781 DCENCERLPMLGQLTNLEELCISGLHSVRSIGNEFYG--NDSDQRTFFPKLKKFTLNKMS 840

Query: 841 SLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDEN 879
           +L QWE+  +A N+T +FP +E+  IS+C +LL +P  +FGS D+N
Sbjct: 841 NLEQWEEVTLAPNLT-VFPCIEMWKISECFKLLKVPK-IFGSWDKN 878

BLAST of MC02g1305 vs. ExPASy TrEMBL
Match: A0A6J1FWC6 (disease resistance protein RGA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 606/1016 (59.65%), Postives = 751/1016 (73.92%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            M EFL TFA +E+LKKVV++AA++IGL WGLEK+L  L+EWLLK+E FL DIN RKLH+D
Sbjct: 1    MGEFLWTFAVQEMLKKVVRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHND 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQ-TAKMKKVRNFLSPSNNAFVFRVKMAN 120
            SVRLWV+KL  LVHEA+DL+D LVYE LR KVQ   KM K                    
Sbjct: 61   SVRLWVEKLHHLVHEADDLIDNLVYEHLRHKVQKNDKMNK-------------------- 120

Query: 121  RMKNISDLLCKHYHEAGSLGLVASESAET--EIDISQLRETVSELDDFDVVGRDVEVSSI 180
                    L KH  EA  LGLV  E  +T  + D+SQ+RETVSE+DDF+VVGRDVEVSSI
Sbjct: 121  --------LNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSI 180

Query: 181  VNQVMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKI 240
            V QV+DA N+ VTSILPIVGMGGLGKTTLA+L+F+HE I++HFD+TIWVCVS PF +NKI
Sbjct: 181  VKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKI 240

Query: 241  LGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRIT 300
            LGAIL+ ++G      N+E+LL ELKKEM+GKRYFLVLDDVWNE+  LW++LKN L +IT
Sbjct: 241  LGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKIT 300

Query: 301  EESENSILVTTRSIEVAKIME-TLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQ 360
             +S NSILVTTR++EV KIME  LP++H++KLSD+QCW LF ESANANGLP TS+L+  +
Sbjct: 301  SKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVR 360

Query: 361  EGLVKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLF--QEENFALSILKLSVDR 420
            E LVKK GG+PLVA+VLG  VKF+ ++DIWV++LE  + +    Q +NF L ILKLSVD 
Sbjct: 361  EELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDY 420

Query: 421  LPSFSLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRS-NKTIEDEGDRYFKIL 480
            LPS SLK CFAYCSNFPKD+ FRKD LIQMW+AQGFI LQEGRS  +T+E+EG+RYFKIL
Sbjct: 421  LPS-SLKPCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKIL 480

Query: 481  LSRCLFQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKL 540
            LSRCLFQDVVKD RGRII CKMHDLIHD+ACDVSN+ K  LD S  S D ELW    + +
Sbjct: 481  LSRCLFQDVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLD-SCTSSDEELWRNRTKNI 540

Query: 541  ARKLRTIIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDI 600
              KLR I  ++ M PH IE+A+HDK+ +F CLRVLIMNS SI +LP SI +LKHLRY+DI
Sbjct: 541  GSKLRMIACHEGM-PHRIEKAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDI 600

Query: 601  SGCVIKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEFSYTYVVE--QMPPY 660
            SGC+I+KL +SIVLLYNL+TLR   + +  LPK+LR L++LRHLEFS  Y V+  +MPP+
Sbjct: 601  SGCLIQKLPKSIVLLYNLKTLRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPH 660

Query: 661  LSQLIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKEN 720
            LSQLI LQTLSCF+VGF K C+I  LGPLKNLKGSLKI +LERV S+EEAKS+  +EKE 
Sbjct: 661  LSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEK 720

Query: 721  LDELTLSWSWPGE-RNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVE 780
            L EL L W W  E  ++ +NH  ++VLEGLQP K L +L I  F GEC  N IF++NLV 
Sbjct: 721  LRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVA 780

Query: 781  ISLCSCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRV 840
            ISL  C NC RLPMLG L +L+ L +  L  VRSIG+EFYG  + SNQ+ LF K+K F++
Sbjct: 781  ISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYG--NHSNQRALFPKVKIFKL 840

Query: 841  EKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTI 900
             ++  L QWE+  V ++VTT FP+L+VL IS C +LL++  D+FG CDENG  HL  + I
Sbjct: 841  IEVERLEQWEEVTVGAHVTT-FPQLQVLEISKCPRLLSI-SDLFGCCDENGVQHLRVLQI 900

Query: 901  SDCIQLDKLPNGLKYCHSIQYLNVM-RLSCLTLKFENVDMVSSLRIMVPQKLAHRTNL-E 960
             +C +L KLPNGL++CHSIQ+L ++   S L L  +N  ++  L    P++LAH + L E
Sbjct: 901  LECSKLTKLPNGLQFCHSIQHLRLIDNCSFLALDLQNEHLLMRL----PERLAHLSELKE 960

Query: 961  LNIYGSIQYDRFYN-RDCEE-LELVDELESGSQDPSSIQSLPEWLDNLTSLQTLNL 1001
            ++I G  Q+    N R     ++L+   E   QD S IQSLPEWL+N TSLQ LNL
Sbjct: 961  MDIVGCTQHGDLMNLRPVRPVIKLLSNDEHRFQDLSEIQSLPEWLENFTSLQLLNL 977

BLAST of MC02g1305 vs. ExPASy TrEMBL
Match: A0A6J1D340 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111017143 PE=4 SV=1)

HSP 1 Score: 1085 bits (2806), Expect = 0.0
Identity = 607/1032 (58.82%), Postives = 742/1032 (71.90%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            MAE L T   E+VL+K+  V AEQIG+A G    LEEL + L++ E  +    K+  H D
Sbjct: 1    MAESLWTCVVEKVLEKIASVVAEQIGVATGFNNALEELHKNLVEAENIINATTKKTSHDD 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANR 120
             +R  V+ LQ  V EA D+LD +VYEDLR+KV++    KV      S  +  FR+K A  
Sbjct: 61   LLRKGVNDLQLTVDEANDVLDLIVYEDLRKKVKSQTKWKVPFSFCTSKYSPAFRLKRAKE 120

Query: 121  MKNISDLLCKHYHEAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQ 180
            +K I  +L +H  +   L  +   + ETEID++QLRET S++DDFD+VGRDVE+SSIV++
Sbjct: 121  IKEILGMLSEHMDKLPKLASLGDNTTETEIDMNQLRETDSKIDDFDIVGRDVELSSIVDR 180

Query: 181  VMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDK-TIWVCVSEPFLVNKILG 240
            V+DATNRHVTSILPIVG+GGLGKTTLAKLV +HERI+ HF+  TIWVCVS+PF VNKILG
Sbjct: 181  VIDATNRHVTSILPIVGVGGLGKTTLAKLVINHERIKHHFNNNTIWVCVSQPFNVNKILG 240

Query: 241  AILETLRGTSDRLD-NKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITE 300
             ILETL+ T   L+  KEVLL +L KEM GK+YFLVLDDVWNEN  LWDELKNCL +ITE
Sbjct: 241  TILETLKDTPSGLEYKKEVLLGQLYKEMRGKKYFLVLDDVWNENRLLWDELKNCLMKITE 300

Query: 301  ESENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQEG 360
             SEN ILVTTRS EVAKIMETLPS+HL KLSDDQCW LFQESANANGL MTSSLKV +E 
Sbjct: 301  NSENGILVTTRSAEVAKIMETLPSHHLGKLSDDQCWSLFQESANANGLSMTSSLKVVREE 360

Query: 361  LVKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLSVDRLPSF 420
            LVKKIGG+PL+AKV G   ++ G+ D WV  L+S +    Q EN A S LKLSVDRLP  
Sbjct: 361  LVKKIGGLPLIAKVWGSAEQYLGDPDKWVINLKSLVCNSLQNENSATSALKLSVDRLPRH 420

Query: 421  SLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCL 480
            SLKQCFAYCSNFPKDF F K+QLIQMW+AQGFIHL +GRSN+TIEDEGDR+FKILL RCL
Sbjct: 421  SLKQCFAYCSNFPKDFEFTKEQLIQMWIAQGFIHLPKGRSNETIEDEGDRHFKILLRRCL 480

Query: 481  FQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKLARKLR 540
            FQDVV+DDRG I+ CKMHDLIHDIACDVSN++KLQ  YS+NSLDGE+WT EN+K+A KLR
Sbjct: 481  FQDVVQDDRGIIVSCKMHDLIHDIACDVSNDHKLQ--YSNNSLDGEVWTDENKKIASKLR 540

Query: 541  TIIYYKE-MIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV 600
            TIIY ++ M+P I+E+ IHDK+MNF CLRVL MNS SI ELP+SI +LKHLRYLDISGC 
Sbjct: 541  TIIYCEDQMMPPIMEKVIHDKVMNFVCLRVLKMNSRSISELPDSIGELKHLRYLDISGCS 600

Query: 601  IKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEF-SYTYVVEQMPPYLSQLI 660
            I +L +SI LLYNLQTL      +  LPKN+ +L++L+HL+F  Y +   +MP Y  +L 
Sbjct: 601  ITQLPKSIALLYNLQTLNMGGLLIKDLPKNIIDLVNLKHLQFYPYHFDTYKMPAYFGKLT 660

Query: 661  QLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDELT 720
            QLQTLS F+VGF  G KI+ LG LKNLKGSL +  LE+V SKEEA+++KLVEKENLDEL 
Sbjct: 661  QLQTLSYFVVGFENGHKISELGLLKNLKGSLNLCFLEKVESKEEARAAKLVEKENLDELE 720

Query: 721  LSWSWPGERNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVEISLCSC 780
              WS    R + +++KDFE+LEGLQP KNL++L I R   +  PN IF+ENL  ISL  C
Sbjct: 721  FEWSQSHLRKDNDSYKDFEILEGLQPHKNLRSLSIERSAIKSLPNNIFVENLAVISLRDC 780

Query: 781  SNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRVEKMRSL 840
             NCERLPMLG+L NL+ L +  L  V+SI NEFYG  +D NQ+TLF KL+ F +  M +L
Sbjct: 781  QNCERLPMLGQLRNLERLEVYGLHSVQSISNEFYG--NDCNQRTLFPKLRAFLLSDMENL 840

Query: 841  VQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTISDCIQL 900
             QW++  VASN+TT FP LE L I  C +L+N+PD VFG CDENG  HL  VTIS C  L
Sbjct: 841  EQWKEVTVASNLTT-FPCLESLNIWRCPKLMNIPD-VFGYCDENGVEHLRHVTISGCDGL 900

Query: 901  DKLPNGLKYCHSIQYLNVMRLSCLTLKFENVDMVSSLRI----MVPQKLAHRTNLE-LNI 960
            +KLPNGL++CHSI+ L +   S LTL  +N + +SSL I     +P+ +AH   LE + I
Sbjct: 901  NKLPNGLQFCHSIEDLTIEDCSNLTLSLQNKNRLSSLSIGGLHKLPEGMAHLKKLEAMKI 960

Query: 961  YGSIQ-YD---RFYNRDCEELELVDELESGSQDPSS-----------------IQSLPEW 1001
             G IQ YD     +      L L D+L + +  P                   I++LPEW
Sbjct: 961  NGGIQDYDFSPLMHLPSLTYLHLCDDLGTATHVPRQVEHCTTLKFLIIENFEHIEALPEW 1020

BLAST of MC02g1305 vs. ExPASy TrEMBL
Match: A0A6J1FYJ5 (disease resistance protein RGA2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448744 PE=4 SV=1)

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 591/1016 (58.17%), Postives = 724/1016 (71.26%), Query Frame = 0

Query: 1    MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSD 60
            M EFL TFA +E+LKKVV++AA++IGL WGLEK+L  L+EWLLK+E FL DIN RKLH+D
Sbjct: 1    MGEFLWTFAVQEMLKKVVRLAAKEIGLTWGLEKELSNLKEWLLKSETFLLDINTRKLHND 60

Query: 61   SVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQ-TAKMKKVRNFLSPSNNAFVFRVKMAN 120
            SVRLWV+KL  LVHEA+DL+D LVYE LR KVQ   KM KV NFL PSNN+ VFR KMA 
Sbjct: 61   SVRLWVEKLHHLVHEADDLIDNLVYEHLRHKVQKNDKMNKVHNFLFPSNNSLVFRYKMAK 120

Query: 121  RMKNISDLLCKHYHEAGSLGLVASESAET--EIDISQLRETVSELDDFDVVGRDVEVSSI 180
            +M N  + L KH  EA  LGLV  E  +T  + D+SQ+RETVSE+DDF+VVGRDVEVSSI
Sbjct: 121  KMNNFMNQLNKHDREATRLGLVGDEPIQTLLQSDVSQIRETVSEVDDFEVVGRDVEVSSI 180

Query: 181  VNQVMDATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKI 240
            V QV+DA N+ VTSILPIVGMGGLGKTTLA+L+F+HE I++HFD+TIWVCVS PF +NKI
Sbjct: 181  VKQVIDAHNQRVTSILPIVGMGGLGKTTLARLIFNHEMIKQHFDETIWVCVSIPFDINKI 240

Query: 241  LGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRIT 300
            LGAIL+ ++G      N+E+LL ELKKEM+GKRYFLVLDDVWNE+  LW++LKN L +IT
Sbjct: 241  LGAILQNVKGHDGCYHNREILLRELKKEMYGKRYFLVLDDVWNEDSLLWEQLKNFLMKIT 300

Query: 301  EESENSILVTTRSIEVAKIME-TLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVFQ 360
             +S NSILVTTR++EV KIME  LP++H++KLSD+QCW LF ESANANGLP TS+L+  +
Sbjct: 301  SKSGNSILVTTRNVEVCKIMEKALPTHHITKLSDEQCWSLFMESANANGLPTTSNLEAVR 360

Query: 361  EGLVKKIGGVPLVAKVLGRTVKFQGNFDIWVTILESEISTLF--QEENFALSILKLSVDR 420
            E LVKK GG+PLVA+VLG  VKF+ ++DIWV++LE  + +    Q +NF L ILKLSVD 
Sbjct: 361  EELVKKFGGIPLVARVLGSAVKFEQDYDIWVSLLEGVLRSPLHLQGKNFCLPILKLSVDY 420

Query: 421  LPSFSLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRS-NKTIEDEGDRYFKIL 480
            LPS SLK CFAYCSNFPKD+ FRKD LIQMW+AQGFI LQEGRS  +T+E+EG+RYFKIL
Sbjct: 421  LPS-SLKPCFAYCSNFPKDYEFRKDPLIQMWIAQGFIQLQEGRSCYQTVEEEGERYFKIL 480

Query: 481  LSRCLFQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGELWTKENQKL 540
            LSRCLFQDVVKD RGRII CKMHDLIHD+ACDVSN+ K  LD S  S D ELW    + +
Sbjct: 481  LSRCLFQDVVKDARGRIISCKMHDLIHDMACDVSNDQKSLLD-SCTSSDEELWRNRTKNI 540

Query: 541  ARKLRTIIYYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDI 600
              KLR I  ++ M PH IE+A+HDK+ +F CLRVLIMNS SI +LP SI +LKHLRY+DI
Sbjct: 541  GSKLRMIACHEGM-PHRIEKAVHDKIGSFVCLRVLIMNSRSIYKLPYSIGELKHLRYVDI 600

Query: 601  SGCVIKKLSESIVLLYNLQTLRAEDTNVL-LPKNLRELISLRHLEFSYTYVVE--QMPPY 660
            SGC+I+KL +SIVLLYNL+TLR   + +  LPK+LR L++LRHLEFS  Y V+  +MPP+
Sbjct: 601  SGCLIQKLPKSIVLLYNLKTLRIGSSPIEELPKDLRRLVNLRHLEFSEIYFVDPKKMPPH 660

Query: 661  LSQLIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKEN 720
            LSQLI LQTLSCF+VGF K C+I  LGPLKNLKGSLKI +LERV S+EEAKS+  +EKE 
Sbjct: 661  LSQLIHLQTLSCFMVGFEKSCEIAELGPLKNLKGSLKISNLERVKSREEAKSANFMEKEK 720

Query: 721  LDELTLSWSWPGE-RNNENNHKDFEVLEGLQPPKNLQTLDIRRFPGECFPNKIFLENLVE 780
            L EL L W W  E  ++ +NH  ++VLEGLQP K L +L I  F GEC  N IF++NLV 
Sbjct: 721  LRELVLEWGWSNETEDHSSNHNGYQVLEGLQPHKKLHSLSIHSFVGECLANNIFVDNLVA 780

Query: 781  ISLCSCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGGISDSNQKTLFSKLKKFRV 840
            ISL  C NC RLPMLG L +L+ L +  L  VRSIG+EFYG  + SNQ            
Sbjct: 781  ISLVDCRNCGRLPMLGHLNSLEELLVSGLNGVRSIGSEFYG--NHSNQ------------ 840

Query: 841  EKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHLTTVTI 900
                                                                 HL  + I
Sbjct: 841  -----------------------------------------------------HLRVLQI 900

Query: 901  SDCIQLDKLPNGLKYCHSIQYLNVM-RLSCLTLKFENVDMVSSLRIMVPQKLAHRTNL-E 960
             +C +L KLPNGL++CHSIQ+L ++   S L L  +N  ++  L    P++LAH + L E
Sbjct: 901  LECSKLTKLPNGLQFCHSIQHLRLIDNCSFLALDLQNEHLLMRL----PERLAHLSELKE 942

Query: 961  LNIYGSIQYDRFYN-RDCEE-LELVDELESGSQDPSSIQSLPEWLDNLTSLQTLNL 1001
            ++I G  Q+    N R     ++L+   E   QD S IQSLPEWL+N TSLQ LNL
Sbjct: 961  MDIVGCTQHGDLMNLRPVRPVIKLLSNDEHRFQDLSEIQSLPEWLENFTSLQLLNL 942

BLAST of MC02g1305 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 378.3 bits (970), Expect = 2.0e-104
Identity = 309/999 (30.93%), Postives = 504/999 (50.45%), Query Frame = 0

Query: 32  EKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLVHEAEDLLDELVYEDLRRK 91
           E  LE L   LL   A L D  ++++ +  V  WV++L+D+V+ AED LD++  E LR  
Sbjct: 36  ENLLERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLN 95

Query: 92  V-----QTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYHEAGSLGLVASESA 151
           +      + +++++R  +S  +        +  R++ ++  L +   +   LGL      
Sbjct: 96  IGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGL----KE 155

Query: 152 ETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMDATNR-HVTSILPIVGMGGLGKTT 211
            T +   Q   T S +D+ +V GRD +   I+  ++    + +  +++ IVG+GG+GKTT
Sbjct: 156 LTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTT 215

Query: 212 LAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLDNKEVLLCELKKE 271
           L++L+++ + +R +F   +W  VSE F V KI   + E++        + +VL  +LK+ 
Sbjct: 216 LSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKER 275

Query: 272 MHGK--RYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTTRSIEVAKIMETLPSY 331
           + G    + LVLDD+WNEN + WD L+       + S+  ILVTTRS  VA IM  +  +
Sbjct: 276 LTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQ--ILVTTRSQRVASIMCAVHVH 335

Query: 332 HLSKLSDDQCWYLFQESANANGLP-MTSSLKVFQEGLVKKIGGVPLVAKVLGRTVKFQGN 391
           +L  LSD  CW LF ++   N  P +   +    E +V K  G+PL  K LG  ++F+G 
Sbjct: 336 NLQPLSDGDCWSLFMKTVFGNQEPCLNREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGK 395

Query: 392 FDIWVTILESEISTLFQEENFALSILKLSVDRLPSFSLKQCFAYCSNFPKDFVFRKDQLI 451
              W  +L S I  L  +++  L +L++S   LP+  LK+CFAYCS FPK   F KD+++
Sbjct: 396 VIEWERVLSSRIWDLPADKSNLLPVLRVSYYYLPA-HLKRCFAYCSIFPKGHAFEKDKVV 455

Query: 452 QMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDVVKDDRGRIIECKMHDLIHDI 511
            +WMA+GF  LQ+ RS+K +E+ G+ YF  L SR L Q      + R I   MHD I+++
Sbjct: 456 LLWMAEGF--LQQTRSSKNLEELGNEYFSELESRSLLQKT----KTRYI---MHDFINEL 515

Query: 512 ACDVSNENKLQLDYSSNSLDG-ELWTKENQKLA-------------RKLRTIIYYKEMIP 571
           A   S E      +SS   DG +L   E  +                 LR + + +  +P
Sbjct: 516 AQFASGE------FSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLP 575

Query: 572 ---------HIIERAIHDKMM-NFFCLRVLIMNSNSIKELPNSIHK-LKHLRYLDISGCV 631
                      +++ + +K++     LRVL ++   I  LP    K + H R+LD+S   
Sbjct: 576 LSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTE 635

Query: 632 IKKLSESIVLLYNLQTLRAEDTNVL--LPKNLRELISLRHLEFSYTYVVEQMPPYLSQLI 691
           ++KL +S+  +YNLQTL     + L  LP ++  LI+LR+L+   T  + QMP    +L 
Sbjct: 636 LEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGT-KLRQMPRRFGRLK 695

Query: 692 QLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKIFHLERVGSKEEAKSSKLVEKENLDELT 751
            LQTL+ F V    G +I+ LG L +L G LKI  L+RV    +A  + L  K++L E+ 
Sbjct: 696 SLQTLTTFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREID 755

Query: 752 LSW--SWPGERNNENNHK---DFEVLEGLQPPKNLQTLDIRRFPGECFPNKI---FLENL 811
             W        NN N H+   + EV E L+P ++++ L I R+ G  FP+ +       +
Sbjct: 756 FVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRI 815

Query: 812 VEISLCSCSNCERLPMLGELPNLDSLCIIELTKVRSIGNEFYGG---ISDSNQKTLFSKL 871
           V I L  C  C  LP LG+LP L  L I  +  ++SIG +FY     + D +Q+  F  L
Sbjct: 816 VCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQP-FRSL 875

Query: 872 KKFRVEKMRSLVQWEDSKVASNVTTLFPRLEVLYISDCHQLLNLPDDVFGSCDENGDYHL 931
           +  R + +    +W D +V      LFP L+ L+I  C +L         S        L
Sbjct: 876 ETLRFDNLPDWQEWLDVRVTRG--DLFPSLKKLFILRCPELTGTLPTFLPS--------L 935

Query: 932 TTVTISDCIQLDKLPNGLKYCH-SIQYLNVMRLSCLTLKFENVDMVSSLRIMVPQKLAHR 980
            ++ I  C  LD  P+  +Y + ++Q L++ + SC TL    ++  ++L  +   +    
Sbjct: 936 ISLHIYKCGLLDFQPDHHEYSYRNLQTLSI-KSSCDTLVKFPLNHFANLDKLEVDQCTSL 995

BLAST of MC02g1305 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-89
Identity = 277/933 (29.69%), Postives = 459/933 (49.20%), Query Frame = 0

Query: 1   MAEFLLTFAAEEVLKKVVKVAAEQIGLAWGLEKD--LEELREWLLKTEAFLRDINKRKLH 60
           MA   L+  A  ++++ +  + E + L  G      L+ L+  L+     L D ++R  H
Sbjct: 1   MANSYLSSCANVMVER-INTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEH 60

Query: 61  SDSVRLWVDKLQDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMA 120
              V+ W+  ++D   +AED+LDEL  E LRR+V          F +        + K+ 
Sbjct: 61  VREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQKKIE 120

Query: 121 NRMKNISDLLCKHYHEAGSLGLVA-SESAETEIDISQLRETVSELDDFDVVGRDVEVSSI 180
            +M+ +  LL  H      +GL   SE+ E +   +  R    +L    +VGR  +  ++
Sbjct: 121 PKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQAS-RSRPDDLPQGRLVGRVEDKLAL 180

Query: 181 VNQVM--DATNRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVN 240
           VN ++  D  +    +++ +VGM G+GKTTL ++VF+  R+ +HF+  +W+     F V 
Sbjct: 181 VNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVF 240

Query: 241 KILGAILETLRGTSDRLDNKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTR 300
            +  A+L+ +  ++   ++   L  +LKK + GKR+ LVLDD W+E+ S W+  +   T 
Sbjct: 241 TVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWESFQVAFTD 300

Query: 301 ITEESENSILVTTRSIEVAKIMETLPSYHLSKLSDDQCWYLFQESANANGLPMTSSLKVF 360
             E S+  I++TTRS  V+ + +    Y +  +++++CW L    A  N      S+   
Sbjct: 301 AEEGSK--IVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGN--ISVGSINQE 360

Query: 361 QEGLVKKIG----GVPLVAKVLGRTVKFQGNFDIWVTILESEISTLFQEENFALSILKLS 420
            EG+ K+I     G+PL A+ +   ++ + N D W  + ++  S      N  L +LKLS
Sbjct: 361 LEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKNFSS----YTNSILPVLKLS 420

Query: 421 VDRLPSFSLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFK 480
            D LP   LK+CFA CS FPK  VF +++L+ +WMA   ++  + RS++ +ED G+ Y  
Sbjct: 421 YDSLPP-QLKRCFALCSIFPKGHVFDREELVLLWMAIDLLY--QPRSSRRLEDIGNDYLG 480

Query: 481 ILLSRCLFQ--DVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLD-------------- 540
            L+++  FQ  D+       +    MHDL++D+A  VS +   +L+              
Sbjct: 481 DLVAQSFFQRLDIT------MTSFVMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHF 540

Query: 541 -YSSNSLDGELWTKENQKLARKLRTIIYYKEMIP----HIIERAIHDKMMNFFCLRVLIM 600
            +S +  D  +  +     A  LRTI+ +          + E+ ++  +     LR+L +
Sbjct: 541 SFSRSQCDASVAFRSICG-AEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSL 600

Query: 601 NSNSIKELPNSIHKLKHLRYLDISGCVIKKLSESIVLLYNLQTLRAEDTNVL--LPKNLR 660
           +   I  LP S+  LK LRYLD+S   IK+L E +  L NLQTL   +   L  LPK++ 
Sbjct: 601 SHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIA 660

Query: 661 ELISLRHLEFSYTYVVEQMPPYLSQLIQLQTLSCFLVGFGKGCKITXLGPLKNLKGSLKI 720
           ELI+LR L+   T +VE MPP + +L  LQ LS F++G   G  +  L  L +L+G+L+I
Sbjct: 661 ELINLRLLDLVGTPLVE-MPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRI 720

Query: 721 FHLERVGSKEEAKSSKLVEKENLDELTLSWSWPGE-----RNNENNHKDFEVLEGLQPPK 780
             L+ V    EAK + L  K  LD L L W+  G        N       EVL  L+P  
Sbjct: 721 SELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHP 780

Query: 781 NLQTLDIRRFPGECFPNKI---FLENLVEISLCSCSNCERLPMLGELPNLDSLCIIELTK 840
           +L+T  I  + G  FP  +       +  ++L SC+ C  LP +G+LP+L  L I +   
Sbjct: 781 HLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNI 840

Query: 841 VRSIGNEFYGGISDSNQKTLFSKLKKFRVEKMRSLVQWEDSKVASNVTTLFPRLEVLYIS 893
           ++ +G +F+ G ++S         +  ++ K   + +W++         +FP L+ L I 
Sbjct: 841 LQKVGLDFFFGENNSRG----VPFQSLQILKFYGMPRWDEWICPELEDGIFPCLQKLIIQ 899

BLAST of MC02g1305 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 221.1 bits (562), Expect = 4.0e-57
Identity = 222/810 (27.41%), Postives = 380/810 (46.91%), Query Frame = 0

Query: 14  LKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLV 73
           L+K + +  E+        K LE+L+  L   ++FL+D  ++K  ++++R  V  L++LV
Sbjct: 10  LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69

Query: 74  HEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYH 133
           +EAED+L +    D     +        + L P+      + K + R++ I++ + K   
Sbjct: 70  YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPAR--VPLQYKKSKRLQEINERITKIKS 129

Query: 134 EAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMDATNRHVTSIL 193
           +        + S     D    R +    D   VVG + +   I  + +  +N     I+
Sbjct: 130 QVEPYFEFITPS-NVGRDNGTDRWSSPVYDHTQVVGLEGDKRKI-KEWLFRSNDSQLLIM 189

Query: 194 PIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLD 253
             VGMGGLGKTT+A+ VF+ + I   F++ IWV VS+ F   +I+ +IL  L G +   D
Sbjct: 190 AFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNL-GDASVGD 249

Query: 254 NKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTTRSIEV 313
           +   LL ++++ + GKRY +V+DDVW++N S WD++   L R       S++VTTRS  V
Sbjct: 250 DIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR---GQGGSVIVTTRSESV 309

Query: 314 AKIMETL--PSYHLSKLSDDQCWYLFQESANA--NGLPMTSSLKVFQEGLVKKIGGVPLV 373
           AK ++     ++    LS D  W LF   A A  +G      L+   + +V K  G+PL 
Sbjct: 310 AKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLT 369

Query: 374 AKVLGRTVKFQGN-FDIWVTILE---SEISTLFQEENFALSILKLSVDRLPSFSLKQCFA 433
            K +G  +  + + +  W  I E    E+     E +  +S L+LS D LPS  LK C  
Sbjct: 370 IKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPS-HLKSCIL 429

Query: 434 YCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDVVKD 493
             S +P+D V  K QL+  W+ +GF+  + GRS     + G+  F  L +RCL + V K 
Sbjct: 430 TLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRS---ATESGEDCFSGLTNRCLIEVVDKT 489

Query: 494 DRGRIIECKMHDLIHDIACDVSNENKLQ----LDYSSNSLDGELWTKENQKLARKLRTII 553
             G II CK+HD++ D+  D++ ++       L+     + G  + ++  K+  KLR ++
Sbjct: 490 YSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGN-FDEKQIKVNHKLRGVV 549

Query: 554 YYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV---- 613
              +                     V  +NS+  K+  +     K+LR LDIS  +    
Sbjct: 550 STTK------------------TGEVNKLNSDLAKKFTD----CKYLRVLDISKSIFDAP 609

Query: 614 IKKLSESIVLLYNLQTLRAEDTNVLL--PKNLRELISLRHLEFSYTYVVEQMPPYL---S 673
           + ++ + I  L +L  L   +T+ L+  P+++ +L +L+ L+ SY   ++Q+ P +    
Sbjct: 610 LSEILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFK 669

Query: 674 QLIQLQ-----TLSCFLVGFG-----------------KGCKITXLGPLKNLKGSLKI-F 733
           +L+ L      +L CF  G G                  GCK   L  +KNL    K+  
Sbjct: 670 KLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCK---LSEVKNLTNLRKLGL 729

Query: 734 HLERVGSKEEAKSSKLVEKENLDELTLSWSWPGERNNENNHKD--FEVLEGLQPPKNLQT 775
            L R    EE +   L+    L  +++        N  +++ D     ++ L PP  L  
Sbjct: 730 SLTRGDQIEEEELDSLINLSKLMSISI--------NCYDSYGDDLITKIDALTPPHQLHE 773

BLAST of MC02g1305 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 221.1 bits (562), Expect = 4.0e-57
Identity = 222/810 (27.41%), Postives = 380/810 (46.91%), Query Frame = 0

Query: 14  LKKVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSVRLWVDKLQDLV 73
           L+K + +  E+        K LE+L+  L   ++FL+D  ++K  ++++R  V  L++LV
Sbjct: 10  LEKTLNILEEKGRTVSDYRKQLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELV 69

Query: 74  HEAEDLLDELVYEDLRRKVQTAKMKKVRNFLSPSNNAFVFRVKMANRMKNISDLLCKHYH 133
           +EAED+L +    D     +        + L P+      + K + R++ I++ + K   
Sbjct: 70  YEAEDILVDCQLADGDDGNEQRSSNAWLSRLHPAR--VPLQYKKSKRLQEINERITKIKS 129

Query: 134 EAGSLGLVASESAETEIDISQLRETVSELDDFDVVGRDVEVSSIVNQVMDATNRHVTSIL 193
           +        + S     D    R +    D   VVG + +   I  + +  +N     I+
Sbjct: 130 QVEPYFEFITPS-NVGRDNGTDRWSSPVYDHTQVVGLEGDKRKI-KEWLFRSNDSQLLIM 189

Query: 194 PIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETLRGTSDRLD 253
             VGMGGLGKTT+A+ VF+ + I   F++ IWV VS+ F   +I+ +IL  L G +   D
Sbjct: 190 AFVGMGGLGKTTIAQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNL-GDASVGD 249

Query: 254 NKEVLLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITEESENSILVTTRSIEV 313
           +   LL ++++ + GKRY +V+DDVW++N S WD++   L R       S++VTTRS  V
Sbjct: 250 DIGTLLRKIQQYLLGKRYLIVMDDVWDKNLSWWDKIYQGLPR---GQGGSVIVTTRSESV 309

Query: 314 AKIMETL--PSYHLSKLSDDQCWYLFQESANA--NGLPMTSSLKVFQEGLVKKIGGVPLV 373
           AK ++     ++    LS D  W LF   A A  +G      L+   + +V K  G+PL 
Sbjct: 310 AKRVQARDDKTHRPELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLT 369

Query: 374 AKVLGRTVKFQGN-FDIWVTILE---SEISTLFQEENFALSILKLSVDRLPSFSLKQCFA 433
            K +G  +  + + +  W  I E    E+     E +  +S L+LS D LPS  LK C  
Sbjct: 370 IKAVGGLLLCKDHVYHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPS-HLKSCIL 429

Query: 434 YCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFKILLSRCLFQDVVKD 493
             S +P+D V  K QL+  W+ +GF+  + GRS     + G+  F  L +RCL + V K 
Sbjct: 430 TLSLYPEDCVIPKQQLVHGWIGEGFVMWRNGRS---ATESGEDCFSGLTNRCLIEVVDKT 489

Query: 494 DRGRIIECKMHDLIHDIACDVSNENKLQ----LDYSSNSLDGELWTKENQKLARKLRTII 553
             G II CK+HD++ D+  D++ ++       L+     + G  + ++  K+  KLR ++
Sbjct: 490 YSGTIITCKIHDMVRDLVIDIAKKDSFSNPEGLNCRHLGISGN-FDEKQIKVNHKLRGVV 549

Query: 554 YYKEMIPHIIERAIHDKMMNFFCLRVLIMNSNSIKELPNSIHKLKHLRYLDISGCV---- 613
              +                     V  +NS+  K+  +     K+LR LDIS  +    
Sbjct: 550 STTK------------------TGEVNKLNSDLAKKFTD----CKYLRVLDISKSIFDAP 609

Query: 614 IKKLSESIVLLYNLQTLRAEDTNVLL--PKNLRELISLRHLEFSYTYVVEQMPPYL---S 673
           + ++ + I  L +L  L   +T+ L+  P+++ +L +L+ L+ SY   ++Q+ P +    
Sbjct: 610 LSEILDEIASLQHLACLSLSNTHPLIQFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFK 669

Query: 674 QLIQLQ-----TLSCFLVGFG-----------------KGCKITXLGPLKNLKGSLKI-F 733
           +L+ L      +L CF  G G                  GCK   L  +KNL    K+  
Sbjct: 670 KLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSNNGCK---LSEVKNLTNLRKLGL 729

Query: 734 HLERVGSKEEAKSSKLVEKENLDELTLSWSWPGERNNENNHKD--FEVLEGLQPPKNLQT 775
            L R    EE +   L+    L  +++        N  +++ D     ++ L PP  L  
Sbjct: 730 SLTRGDQIEEEELDSLINLSKLMSISI--------NCYDSYGDDLITKIDALTPPHQLHE 773

BLAST of MC02g1305 vs. TAIR 10
Match: AT3G07040.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 202.6 bits (514), Expect = 1.5e-51
Identity = 187/698 (26.79%), Postives = 339/698 (48.57%), Query Frame = 0

Query: 16  KVVKVAAEQIGLAWGLEKDLEELREWLLKTEAFLRDINKRKLHSDSV------RLWVDKL 75
           +++ V   +  L  G+  +++++++ LL  ++FL D +K   +  +       + +V   
Sbjct: 12  RILSVLENETLLLSGVHGEIDKMKKELLIMKSFLEDTHKHGGNGSTTTTTQLFQTFVANT 71

Query: 76  QDLVHEAEDLLDELVYEDLRRKVQTAKMKKVRNF--LSPSNNAFVFRVKMANRM-KNISD 135
           +DL ++ ED+LDE  Y  +      AK+ +  +F     + ++   ++ M N M ++ISD
Sbjct: 72  RDLAYQIEDILDEFGYH-IHGYRSCAKIWRAFHFPRYMWARHSIAQKLGMVNVMIQSISD 131

Query: 136 LLCKHYHEAGSLGLVASESAETEID-ISQLRETVSELDDFDVVGRDVEVSSIVNQVMDAT 195
            + ++YH       +     + +   ++ + E+     +  +VG D     ++ +++   
Sbjct: 132 SMKRYYHSENYQAALLPPIDDGDAKWVNNISESSLFFSENSLVGIDAPKGKLIGRLLSPE 191

Query: 196 NRHVTSILPIVGMGGLGKTTLAKLVFHHERIRKHFDKTIWVCVSEPFLVNKILGAILETL 255
            + +  ++ +VGMGG GKTTL+  +F  + +R+HF+   WV +S+ +++  +   +++  
Sbjct: 192 PQRI--VVAVVGMGGSGKTTLSANIFKSQSVRRHFESYAWVTISKSYVIEDVFRTMIKEF 251

Query: 256 RGTSDRLDNKEV-------LLCELKKEMHGKRYFLVLDDVWNENPSLWDELKNCLTRITE 315
              +D     E+       L+ +L + +  KRY +VLDDVW     LW E+   L     
Sbjct: 252 YKEADTQIPAELYSLGYRELVEKLVEYLQSKRYIVVLDDVW--TTGLWREISIALPDGIY 311

Query: 316 ESENSILVTTRSIEVAKIMETLPS--YHLSKLSDDQCWYLFQESANANGLPM--TSSLKV 375
            S   +++TTR + VA     + S  + +  L +D+ W LF   A    L    T +L+ 
Sbjct: 312 GSR--VMMTTRDMNVASFPYGIGSTKHEIELLKEDEAWVLFSNKAFPASLEQCRTQNLEP 371

Query: 376 FQEGLVKKIGGVPLVAKVLG---RTVKFQGNFDIWVTILESEISTLFQEENFALSILKLS 435
               LV++  G+PL    LG    T KF+  +    + L  E++    E     SI+ LS
Sbjct: 372 IARKLVERCQGLPLAIASLGSMMSTKKFESEWKKVYSTLNWELNN-NHELKIVRSIMFLS 431

Query: 436 VDRLPSFSLKQCFAYCSNFPKDFVFRKDQLIQMWMAQGFIHLQEGRSNKTIEDEGDRYFK 495
            + LP + LK+CF YCS FP ++  ++ +LI+MWMAQ F+    G      E+  D Y  
Sbjct: 432 FNDLP-YPLKRCFLYCSLFPVNYRMKRKRLIRMWMAQRFVEPIRG---VKAEEVADSYLN 491

Query: 496 ILLSRCLFQDVVKDDRGRIIECKMHDLIHDIACDVSNENKLQLDYSSNSLDGE--LWTKE 555
            L+ R + Q ++ +  GR    KMHD+I +IA  VS   +    Y+ +S DG+    T E
Sbjct: 492 ELVYRNMLQVILWNPFGRPKAFKMHDVIWEIALSVSKLERFCDVYNDDS-DGDDAAETME 551

Query: 556 NQKLARKLRTIIYYKEMIPHIIERA-IHDKMM------------NFFCLRVLIMNSNSIK 615
           N       R +   KEM P  I    +H  ++            +   LR L +  +SI 
Sbjct: 552 NY----GSRHLCIQKEMTPDSIRATNLHSLLVCSSAKHKMELLPSLNLLRALDLEDSSIS 611

Query: 616 ELPNSIHKLKHLRYLDISGCVIKKLSESIVLLYNLQTLRAEDTNV-LLP------KNLRE 664
           +LP+ +  + +L+YL++S   +K+L ++   L NL+TL  + + +  LP      K LR 
Sbjct: 612 KLPDCLVTMFNLKYLNLSKTQVKELPKNFHKLVNLETLNTKHSKIEELPLGMWKLKKLRY 671