MC02g1226 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC02g1226
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionShikimate kinase
LocationMC02: 11172706 .. 11178169 (+)
RNA-Seq ExpressionMC02g1226
SyntenyMC02g1226
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCATTATTATCAAATATTATAAACAATTATAAAAGTCTGCGGCTTCTGTTGAAGGTTCATTTCAGATATTATAAAAATGGAAAATATGAAATAATTGCAGAACGAAGTCTTGGAATTGGAGCTGAGAGAAGTAGCAGTTGCGAACCAGAGGAGCATATTGAAGTCCACGCCGAGGGAGAGGTTGGAGCTCGAGGATGTTCTCTGTGTTCAATCTGTTCTAGATCATCGCATATATTTGAGATTCAAACTCCGGGTACGATCACTTGCCACTATACCTTGGAAGCTTTCATGAACATTTGATTTGATCACGCTCAAAAAGAAAAAAAAAGTTTGTGTTTATTTTTTGATTTCATTTCGTGAGGTGTTTTTTCTCTTCGGAATTTTGATCTTGAGGTTCTGTTCTCATGGAATTAGGTGGTGTTTTTGTTTTACGCCACGTATTTTTCCGATTCGGTTCTGATTTTGGTTTTATTCCGGAAATTAATGGATTGGGAAATCTAATGTGACAACCTCCGATTGCAGCGGTTTTCTTCGCTACGGTTTGTTCTTGAAATTTGGTTGAATCGAACTGCTATGCAAGCAAGTATGGAGGCTACGGTTACGCAGCAGCTACAATTTTCTCCGTGGATCAACTCCAAGAAGATTGCTGCAAAACCGAAGGGCTCGCTGCACTTTTCTCGGCGATTAATAGAGCAGCAGATGTTGCAGGCGCCGTTTTCATTCAATCTACAACCGTCAAGACCTCCAAATCACCACAGAACAGCCGCTTTGAAAATTTCCTGTTCTTACAAGAACTCTACAGGTTTGCTGTTAGCGATTTATAATCCATTCATTCAATTTTCTCTTCTTTCTGCTCAAGAAACGGTTACCTATTAATTCGATGGTATTCTCTAGAATTATGCCCAATTTGGGTAAAAGCCAACCAGACCTTAGCTCATCGAATTTTGACTAGCAGTTTTCTTCACTCCAGCGACGTCTACACGTGCTAAAATTAATCTGCTCCAATCTGTTCTTTACCCGTGCTAAAATTAATTTTCCAAAACACGTGAAGTCCCTCATCTTTGTTATTTACCCGTGGTGAAATTAATTTGCTCCAATTTGTTCAAATTTGCACTCTATACTTGGAACAACTTGGAGCACCATTATCAAATGTATTATAAAATGACAATTTCATTTCAACATGATTTTGCTTCGTCGTGAAGTATTTATTTACACATATAGTTGCTTTAGGCACTTGTCAGATTCAGTAGTCTGGAACGCGTGTATTAGGAGTTGGATGAGCGTCTGATGTAGAGTCTGTTAGCAGAATTTTTTGTTTGATATTTTCCGTTGTGAATTCTGGTATTCTTTGGTTGGTTATTTTCCAGCCTGTGAACATTTGATTTGACCATGGTCAAAAAGAAAGATAAAGAGAGAGAATCTTGTTTACGACTTTATGGTTGGTTAGCCTATATATAACTGTTGAGTTAATTAACCTTAACATTTCTCAATCCATTCTGATTCTGAAATATTTTGAAAGTTTCCAATTATTTTCTTTTGCCCAGTTAGAGAATTTACTGCTTTCTCCCTAATCATTGGACTGAGTTTGGGCCCCTTGTCCTCATTTCTAGCTACAAAACTATATTTGCGAAGGAGAATTTTTCTATTAGAAGAAATTTTCTAAGAAGATTTTAACTTTTACTTTCATGACTCCAGCGTTGGAATCTGGAAACCATTTTGCATTTGTTGACGAATCTCTGACTTTACAGGTATGGTTTTTCTCTTTTTTATTCTTATTTTGCAAGTAGTTACTGAAATTGCTTCCTTTCCTTATGTAATTGTGGGCCTATTGTTTACTTATTTCCTGCTATTAGTTGTTGAGCATTTTTTTCATCTCTAGCCTCACTGGTCATAAATTTGCAGCGGAAGGCCCGGGAGATTGAGCCATATTTAAATGGGCATTGTATTTATCTTGTTGGTGAGTTTCTTTCTTGGAGTACTTCATTACTTTTGAGCTTGTGCAATTAATATTTAAATTCTTCCTACCTATGAAATGCAGGAATGATGGGCTCGGGAAAAACCACTGTAGGAAAAGTCTTGTCAAAGGCACTTGGTTACTCGTTTTGTGACAGGTTTGTTTCATAGCTGCAGGCTCTAGCGGATTTACTAACTTTCATGTCAAATCTGTTACGCTCGTTTCAATTTTGATTTCATAGCAAGGGAAAGATTTATTCTTTCAATTTTATAATTAATTTTAACTTCCGAGGTTTATTGAAAAGCAAAAGCCCAGTAAAATGCAGCTGTGAAATTACACTATCATTGAAGCTGCAAGAGATGAAGAAAAATAACTTTGTTTCCACCATTCATTAAGAAAAGAACTTTGGACCTAGGTTTTTTAAGCTATTATGATTCTGGCATCTGTATTTGGCTTTCCTTGTCTCTGTGTCACCGCACACCATCATTGACAATAAATTTTTCTTGCTGATTACTTGTGCTATATTATGTTTTGGATGCCACAGTGATTCATTGGTGGAACAAGACGTTGGAACTTCGGTAGCTGAAATTTTCAAGCTTTATGGGGAGGAATACTTTAGAGAAAAGGAGGTCAGTGTAGATTTGAAATATTCGTAACACCTTGACTTAATTTACTTCGTATGTTCGTTATTATACTCAAAGTTTATGTTGTATGTTAATTACTATGCTTCCTTCTCTCTCTCAAGTGCCTACATACACAGACAACTCTCCCATTATCTTGTAAACGTTTTTAGCCGTTAAGGTTCACACCAGAACAAGGATTTTGAGCTGCTCTCTCTCTCTCTCGACTTATGTATTAGCTAGGATTGATGAAAGTGATTTTATCATATTTCATCATTTGAGATTTTGTTTTTATTTAGTTTAAGGAATCTAATTCTTTCTTAAACTAAAGCCTCAAACTAGCCTCAAAGGTCAAAGCTAATATCACGATGATAGTGGTCTATAATAACGACTATCGATTACTTTGTTTTTATTTATCTTTCTTCTTAAAGATATATCATGAAAAGGGAATTGCCACATGCCAATAATAAGCCCAACCCGTTCTCTAGGCATTAAATTTTGCCATAGACTCTGAACGTGGTATACTACTACAAATTTCTCACAAGCGGCAAGCTCTTTTTTATGCATATCTGAACTCCAGCACAGAATCTTAACTTTTCTCATTAATATAACAGTGAACCTAACTTTTCTTGTTTCATACAGATGGAGTCATTGAGGAAGCTATCTTTGATGAATCAATTAGTTGTTTCCACTGGTGGAGGTGTCGTTACTCGAACGATGAACTGGTATAGTAAAGACTGACTAGTTGGACACGAGTGTTACATTTTCTCCAGTTGTAATTAAGTTTTCAGTGTCATTAGACTGAGCCTTTGTCTTAAACTTTCCAGGACATATATGGATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGAAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCGAATCTCGTCCCCTTTTGCACCAGGATTCAACTGATGCATACTCAAAGGTAGATTTTTTTCTTCATCAATGAAATGTTTCTTATCCACAGAAAAAGAAAGGATATGCTTTTCCTGTACTACCATGATAATGATACCTGACCCAAGTTCATATCACTTGGTACCAGTTTGAGCAAGGAGTTAAATTCAGTGCTTTTTCCCTTCAAGTGCAAATGAAACATGCATGTTATAAACATGATCTCAAAAAGTGTTTTTGTCCTCCTAAAAGAATAAAAAACTACTTGATAAATAATAATTTTAATGTTTAGTTTTAAAATGCACTCTCACATAAAACTTAATCTAATTCCTGTTATTTACAGACCCTCGCCCTTTTATCTACTCTCCTGGAAAAGAGAGGTGAAGCATATGCCAGCGCCCACGTCCAAGTTTCCTGTGAAAGTGAGACATCTCTCCTTTATATGTTATTTATGTATTCTACCAGGGAATGGAGGGAAAAAAAAAACTTTTAAACTAGTCTCTGCTTTTTTTAAGCTTAATTTCTCATATGAAAGCTCAATAATTTTGGTTTTGCAAACCCAATTTATAATTACATTCAAACTACCAACTAAAAGGATGCCATTTAGGGTGAGTTTGAAACATTTTTGGGAGGGTAAAAAGTGATTTTGGCCTATTAAAATACTTTTAAAATTTTTTTTATAGTATTTGGTAGATAGTCGAAAGTGATTTTGAAAATTCTAAGACCACTCCATAGTACTTTTTCAAAATCACCGGTGATACTTTAAAAATCAATTTAAAATAATTTCAAGTTTTTCTAAAATCAATTTTCTAATTTGTCAAACATGATTCATTAGTACTTCTTTAAAAGTGCTTATAAAAGAAATACACACTTAAAAATACTTCTTTCAAAAGTGATCCTAAACTCACCTTAAAAGTGATTCTAAACTCACTTAAAAACACTTTCGCACTTTTTTCAAATGTGATTCTAAACTCACCTTCAATAAAAGTATTGTTAGGCTGTTCATGTACTCTAGGTATCTAAGATCTGCCAATACCAAATGACATAAACCGAGTGTTTCACTTGTGCAGAAATTGCAGCCAAACTGGGCAATAAAGACGCGTCTAACGTCACCCCTATGGCCATAGCAATGGAGGTCTCTCTCTCTCTCTGAACAAAGATGATAATCTACACTACAATTATCCAAAGCGTTTTGACCACGACCCTTTTCATTTCTAACAATTTTTAGCTCGTCCTTGTTTATGTTGTGTTATCCCAGGCACTCGAACAAATCGAGAGTTTCTTGAAGGGACGAGACGATTATTGTGCATTCTAATTTTGACGAACACGAAAAAATTACTTATTCAGGTAATAATAGAAGTTTTACTAAATAGCAGTGAAGTAACTTATCTTTTCACTTGAAATTTCCTTATAAAGTTGTGTGTGTTCTTTTTTTCTGATGTCAGGAACATATGAATAAAGGCAATTGATGAAGCAAATTGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTATTTATGGGGCAATCTTCTCTATCAAAGGCATGCTTCCTCCCCAAGGCTCCCACATTTCTTGACCTCAGAAGTGGTTGGTCTAGATTAATGCTATTTGTACTCCTTTTCACAGAAAATGGAGAGATCCAAATTAGGTATTTTTTGGATTTTGATCCGTCGGGATTAATGGGGCTTGAATCCCTAGGCCACTGCCTTCTTTTAAAAACATTTTGCACCCTGAAATGAGGGGCGAAATGCTATTGATTTTAGGCTCATTGACTGCCGCCCCCCTCGTCTTGGAGGCTCTGCGGTCGAGGAGGGGCTTGCCATTGTAACATTTCAACATTTTACTCAATCAGAAGTAGTGTGGTTTGATTTGAATCTTATAAGATGTACGATGCTCCAAGCACATAGAAAAATTAGCAATTTGTTTGCTTTTAAAGAGTCGGTGAAATTGATCTAAGG

mRNA sequence

ATTCATTATTATCAAATATTATAAACAATTATAAAAGTCTGCGGCTTCTGTTGAAGGTTCATTTCAGATATTATAAAAATGGAAAATATGAAATAATTGCAGAACGAAGTCTTGGAATTGGAGCTGAGAGAAGTAGCAGTTGCGAACCAGAGGAGCATATTGAAGTCCACGCCGAGGGAGAGGTTGGAGCTCGAGGATGTTCTCTGTGTTCAATCTGTTCTAGATCATCGCATATATTTGAGATTCAAACTCCGGCGGTTTTCTTCGCTACGGTTTGTTCTTGAAATTTGGTTGAATCGAACTGCTATGCAAGCAAGTATGGAGGCTACGGTTACGCAGCAGCTACAATTTTCTCCGTGGATCAACTCCAAGAAGATTGCTGCAAAACCGAAGGGCTCGCTGCACTTTTCTCGGCGATTAATAGAGCAGCAGATGTTGCAGGCGCCGTTTTCATTCAATCTACAACCGTCAAGACCTCCAAATCACCACAGAACAGCCGCTTTGAAAATTTCCTGTTCTTACAAGAACTCTACAGCGTTGGAATCTGGAAACCATTTTGCATTTGTTGACGAATCTCTGACTTTACAGCGGAAGGCCCGGGAGATTGAGCCATATTTAAATGGGCATTGTATTTATCTTGTTGGAATGATGGGCTCGGGAAAAACCACTGTAGGAAAAGTCTTGTCAAAGGCACTTGGTTACTCGTTTTGTGACAGTGATTCATTGGTGGAACAAGACGTTGGAACTTCGGTAGCTGAAATTTTCAAGCTTTATGGGGAGGAATACTTTAGAGAAAAGGAGATGGAGTCATTGAGGAAGCTATCTTTGATGAATCAATTAGTTGTTTCCACTGGTGGAGGTGTCGTTACTCGAACGATGAACTGGACATATATGGATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGAAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCGAATCTCGTCCCCTTTTGCACCAGGATTCAACTGATGCATACTCAAAGACCCTCGCCCTTTTATCTACTCTCCTGGAAAAGAGAGGTGAAGCATATGCCAGCGCCCACGTCCAAGTTTCCTGTGAAAAAATTGCAGCCAAACTGGGCAATAAAGACGCGTCTAACGTCACCCCTATGGCCATAGCAATGGAGGCACTCGAACAAATCGAGAGTTTCTTGAAGGGACGAGACGATTATTGTGCATTCTAATTTTGACGAACACGAAAAAATTACTTATTCAGGAACATATGAATAAAGGCAATTGATGAAGCAAATTGGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTATTTATGGGGCAATCTTCTCTATCAAAGGCATGCTTCCTCCCCAAGGCTCCCACATTTCTTGACCTCAGAAGTGGTTGGTCTAGATTAATGCTATTTGTACTCCTTTTCACAGAAAATGGAGAGATCCAAATTAGGTATTTTTTGGATTTTGATCCGTCGGGATTAATGGGGCTTGAATCCCTAGGCCACTGCCTTCTTTTAAAAACATTTTGCACCCTGAAATGAGGGGCGAAATGCTATTGATTTTAGGCTCATTGACTGCCGCCCCCCTCGTCTTGGAGGCTCTGCGGTCGAGGAGGGGCTTGCCATTGTAACATTTCAACATTTTACTCAATCAGAAGTAGTGTGGTTTGATTTGAATCTTATAAGATGTACGATGCTCCAAGCACATAGAAAAATTAGCAATTTGTTTGCTTTTAAAGAGTCGGTGAAATTGATCTAAGG

Coding sequence (CDS)

ATGCAAGCAAGTATGGAGGCTACGGTTACGCAGCAGCTACAATTTTCTCCGTGGATCAACTCCAAGAAGATTGCTGCAAAACCGAAGGGCTCGCTGCACTTTTCTCGGCGATTAATAGAGCAGCAGATGTTGCAGGCGCCGTTTTCATTCAATCTACAACCGTCAAGACCTCCAAATCACCACAGAACAGCCGCTTTGAAAATTTCCTGTTCTTACAAGAACTCTACAGCGTTGGAATCTGGAAACCATTTTGCATTTGTTGACGAATCTCTGACTTTACAGCGGAAGGCCCGGGAGATTGAGCCATATTTAAATGGGCATTGTATTTATCTTGTTGGAATGATGGGCTCGGGAAAAACCACTGTAGGAAAAGTCTTGTCAAAGGCACTTGGTTACTCGTTTTGTGACAGTGATTCATTGGTGGAACAAGACGTTGGAACTTCGGTAGCTGAAATTTTCAAGCTTTATGGGGAGGAATACTTTAGAGAAAAGGAGATGGAGTCATTGAGGAAGCTATCTTTGATGAATCAATTAGTTGTTTCCACTGGTGGAGGTGTCGTTACTCGAACGATGAACTGGACATATATGGATAAAGGGATCAGTGTCTGGTTGGATGTGCCTTTGAAAGCCTTGGTTAAGAGAATTTCAGCTGTAGGAACCGAATCTCGTCCCCTTTTGCACCAGGATTCAACTGATGCATACTCAAAGACCCTCGCCCTTTTATCTACTCTCCTGGAAAAGAGAGGTGAAGCATATGCCAGCGCCCACGTCCAAGTTTCCTGTGAAAAAATTGCAGCCAAACTGGGCAATAAAGACGCGTCTAACGTCACCCCTATGGCCATAGCAATGGAGGCACTCGAACAAATCGAGAGTTTCTTGAAGGGACGAGACGATTATTGTGCATTCTAA

Protein sequence

MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF
Homology
BLAST of MC02g1226 vs. ExPASy Swiss-Prot
Match: Q00497 (Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.5e-81
Identity = 163/293 (55.63%), Postives = 206/293 (70.31%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEA V+Q LQ S WINS K+  KP G L FS +  E+   +   S +LQP +  +  R  
Sbjct: 1   MEARVSQSLQLSSWINSDKVVRKPSGLLRFSEKWNEKPRHRVVVSCHLQPRKAAHSDRRV 60

Query: 65  ALKISCSYKN--STALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTV 124
            LK+SCS +N  ++ LESG   A +DE  TL+ KA E+E YL+G C+YLVGMMG GKTTV
Sbjct: 61  QLKVSCSPQNVQASVLESGCFSASIDEIETLKNKAEEVEEYLDGRCVYLVGMMGCGKTTV 120

Query: 125 GKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVS 184
           G++L++ LGYSF D D L+EQ V G +VAEIF+L GE +FR+ E E L KLSLM++LVVS
Sbjct: 121 GRILAETLGYSFFDCDRLIEQAVGGITVAEIFELRGESFFRDNETEVLHKLSLMHRLVVS 180

Query: 185 TGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALL 244
           TGGG V R +NW +M KGISVWLDVPL+AL KRI+  GT+SRPLLH++S D Y  TL  L
Sbjct: 181 TGGGAVVRPINWRHMHKGISVWLDVPLEALAKRITTEGTKSRPLLHEESGDVYDTTLKRL 240

Query: 245 STLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLK 295
           +TL+E RGE YA+A  +VS E IA K   KD  ++TP  I +E L QIE+FLK
Sbjct: 241 TTLMETRGENYANASARVSLENIALK-REKDVCHITPAEITLEVLIQIENFLK 292

BLAST of MC02g1226 vs. ExPASy Swiss-Prot
Match: Q9SJ05 (Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK1 PE=1 SV=2)

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-78
Identity = 157/293 (53.58%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKP-KGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRT 64
           MEA +TQ++Q+  W++ +K+  KP +GSL +S+++   +  +      LQP R  +  R 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  AALKISCSYKNSTA-LESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTV 124
            +  +SCS  NS+A LE+G+ + F DE + L+RKA E++PYLNG  +YLVGMMGSGKTTV
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPF-DEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTV 120

Query: 125 GKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVS 184
           GK++SK LGY+F D D+L+EQ + GTSVAEIF  +GE +FR KE ++L+KLS   Q+VVS
Sbjct: 121 GKLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVS 180

Query: 185 TGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALL 244
           TGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT+SRPLLH +S DAYS     L
Sbjct: 181 TGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRL 240

Query: 245 STLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLK 295
           S + ++RGEAY +A+ +VS E IAAK G K+ S++TP  IA+EA EQ+ SFL+
Sbjct: 241 SAIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIEAFEQVLSFLE 291

BLAST of MC02g1226 vs. ExPASy Swiss-Prot
Match: Q8GY88 (Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK2 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.4e-73
Identity = 155/299 (51.84%), Postives = 206/299 (68.90%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEA   Q+ Q+S W + +    KP+GSL ++ + I++       +  L   R    HR  
Sbjct: 1   MEAATVQRFQYSSWNDLRNFEGKPRGSLRYNTQRIKEDKRFRVVALTLDKRR---DHRLR 60

Query: 65  ALKISCSYKNSTA-LESGN--HFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTT 124
               S S KNS+A LE+G+  H  F +E   L++KA E++PYLNG  +YLVGMMGSGKTT
Sbjct: 61  ----SVSDKNSSALLETGSLLHSPFDEEQQILKKKAEEVKPYLNGRSMYLVGMMGSGKTT 120

Query: 125 VGKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLM-NQLV 184
           VGK+++++LGY+F D D+L+EQ + GTSVAEIF+ +GE  FREKE E+L+KLSLM +Q+V
Sbjct: 121 VGKIMARSLGYTFFDCDTLIEQAMKGTSVAEIFEHFGESVFREKETEALKKLSLMYHQVV 180

Query: 185 VSTGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQD-STDAYSKTL 244
           VSTGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT SRPLLH D S D Y+  L
Sbjct: 181 VSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRPLLHDDESGDTYTAAL 240

Query: 245 ALLSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRD 298
             LST+ + RGEAY  A  +VS E I  KLG +  S++TP  IA+EA EQ++S+L+  D
Sbjct: 241 NRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAIEAFEQVQSYLEKED 292

BLAST of MC02g1226 vs. ExPASy Swiss-Prot
Match: Q7X7H9 (Shikimate kinase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK3 PE=1 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 3.1e-66
Identity = 132/231 (57.14%), Postives = 175/231 (75.76%), Query Frame = 0

Query: 64  AALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVG 123
           A LK+SCS K +   ++   +   DE+L L++KA ++ PYLN  CIYLVGMMGSGKTTVG
Sbjct: 52  AKLKVSCSRKPAGIDKT---YYSADEALVLKQKAEDVVPYLNDRCIYLVGMMGSGKTTVG 111

Query: 124 KVLSKALGYSFCDSDSLVEQDVG-TSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVST 183
           K+L++ LGYSF DSD LVE+ VG +SVAEIF+L+ E +FR+ E E LR LS M++LVV+T
Sbjct: 112 KILAEVLGYSFFDSDKLVEKAVGISSVAEIFQLHSEAFFRDNESEVLRDLSSMHRLVVAT 171

Query: 184 GGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLS 243
           GGG V R +NW+YM KG ++WLDVPL AL +RI+AVGT SRPLLHQ+S D Y+K  A L+
Sbjct: 172 GGGAVIRPINWSYMKKGSTIWLDVPLDALARRIAAVGTASRPLLHQESGDPYAKAYAKLT 231

Query: 244 TLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFL 294
            L E+R ++YA+A  +VS E IA K G+ + + +TP AIA+EAL ++ESFL
Sbjct: 232 ALFEQRMDSYANADARVSLEHIAVKQGHSNVTTLTPSAIAIEALLKMESFL 279

BLAST of MC02g1226 vs. ExPASy Swiss-Prot
Match: Q5NTH4 (Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK1 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.5e-63
Identity = 128/218 (58.72%), Postives = 162/218 (74.31%), Query Frame = 0

Query: 80  SGNHFAF---VDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGKVLSKALGYSFCD 139
           SG H      VDE+L L+RK+ E+  YLNG CIYLVGMMGSGK+TVGK++S+ LGYSF D
Sbjct: 67  SGGHETLHNSVDEALLLKRKSEEVLFYLNGRCIYLVGMMGSGKSTVGKIMSEVLGYSFFD 126

Query: 140 SDSLVEQDVG-TSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGGGVVTRTMNWTY 199
           SD LVEQ VG  SVA+IFK++ E +FR+ E   LR LS M +LVV+TGGG V R +NW Y
Sbjct: 127 SDKLVEQAVGMPSVAQIFKVHSEAFFRDNESSVLRDLSSMKRLVVATGGGAVIRPVNWKY 186

Query: 200 MDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTLLEKRGEAYASA 259
           M KG+SVWLDVPL AL +RI+ VGT SRPLL Q S D Y+   + LS L E+RG+AYA+A
Sbjct: 187 MKKGLSVWLDVPLDALARRIAKVGTASRPLLDQPSGDPYTMAFSKLSMLAEQRGDAYANA 246

Query: 260 HVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFL 294
            V+VS E+IA+K G+ D S +TP  IA+E+  +IE+F+
Sbjct: 247 DVRVSLEEIASKQGHDDVSKLTPTDIAIESFHKIENFV 284

BLAST of MC02g1226 vs. NCBI nr
Match: XP_022157752.1 (shikimate kinase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 595 bits (1535), Expect = 1.71e-214
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH 60
           MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH
Sbjct: 1   MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH 60

Query: 61  HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT 120
           HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT
Sbjct: 61  HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT 120

Query: 121 TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 180
           TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV
Sbjct: 121 TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 180

Query: 181 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 240
           STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL
Sbjct: 181 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 240

Query: 241 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC 300
           LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC
Sbjct: 241 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC 300

Query: 301 AF 302
           AF
Sbjct: 301 AF 302

BLAST of MC02g1226 vs. NCBI nr
Match: XP_023512754.1 (shikimate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023512755.1 shikimate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481 bits (1239), Expect = 1.87e-169
Identity = 245/298 (82.21%), Postives = 264/298 (88.59%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEATVTQQLQFSPWI+SK IAAKPKGSLHFSRRL EQ MLQAPFSFNLQ SR  +HHRT 
Sbjct: 1   MEATVTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRTV 60

Query: 65  ALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGK 124
           ALKISCSYKNS+ LESGNH   VDE+L +QRK+REIE YLNG CIY VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHRVSVDETLIIQRKSREIESYLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGG 184
           VLS ALGYSF DSDSLVEQDVG SVAEIFK+YGE++FRE+E E+LRKLSLMNQ V+STGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTL 244
           G VTRT+NW YM +GISVWLDVPL+ALVKRISAVGT SRPLLH DSTDAYSKTL  LSTL
Sbjct: 181 GAVTRTINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLIRLSTL 240

Query: 245 LEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF 302
           LE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALEQIE FLK  D YCAF
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

BLAST of MC02g1226 vs. NCBI nr
Match: XP_022971695.1 (shikimate kinase, chloroplastic-like [Cucurbita maxima] >XP_022971696.1 shikimate kinase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 481 bits (1237), Expect = 3.77e-169
Identity = 244/298 (81.88%), Postives = 264/298 (88.59%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEAT+TQQLQFSPWI+SK IAAKPKGSLHFSRRL EQ MLQAPFSFNLQ SR  +HHRT 
Sbjct: 1   MEATLTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRTV 60

Query: 65  ALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGK 124
           ALKISCSYKNS+ LESGNH   VDE+ T+QRK+REIE YLNG CIY VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHRVPVDETFTIQRKSREIESYLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGG 184
           VLS ALGYSF DSDSLVEQDVG SVAEIFK+YGE++FRE+E E+LRKLSLMNQ V+STGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTL 244
           G VTRT+NW YM +GISVWLDVPL+ALVKRISAVGT SRPLLH DSTDAYSKTL  LSTL
Sbjct: 181 GAVTRTINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLVRLSTL 240

Query: 245 LEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF 302
           LE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALEQIE FLK  D YCAF
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

BLAST of MC02g1226 vs. NCBI nr
Match: KAG7011153.1 (Shikimate kinase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 478 bits (1230), Expect = 4.39e-168
Identity = 243/298 (81.54%), Postives = 264/298 (88.59%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEATVTQQLQFSPWI+SK IAAKPKGSLHFSRRL EQ MLQAPFSFNLQ SR  +HHRT 
Sbjct: 1   MEATVTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRTV 60

Query: 65  ALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGK 124
           ALKISCSY+NS+ LESGNH   VDE+LT+QRK+REIE +LNG CIY VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYQNSSVLESGNHRVSVDETLTIQRKSREIESHLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGG 184
           VLS ALGYSF DSDSLVEQDVG SVAEIFK+YGE++FRE+E E+LRKLSLMNQ V+STGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTL 244
           G VTR +NW YM +GISVWLDVPL+ALVKRISAVGT SRPLLH DSTDAYSKTL  LSTL
Sbjct: 181 GAVTRMINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLIRLSTL 240

Query: 245 LEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF 302
           LE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALEQIE FLK  D YCAF
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

BLAST of MC02g1226 vs. NCBI nr
Match: XP_022928030.1 (shikimate kinase, chloroplastic-like [Cucurbita moschata] >XP_022928031.1 shikimate kinase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 476 bits (1224), Expect = 3.60e-167
Identity = 242/298 (81.21%), Postives = 263/298 (88.26%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEATVTQQLQFSPWI+SK IAAKPKGSLHFSRRL EQ MLQAPFSFNLQ SR  +HHR  
Sbjct: 1   MEATVTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRIV 60

Query: 65  ALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGK 124
           ALKISCSY+NS+ LESGNH   VDE+LT+QRK+REIE +LNG CIY VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYQNSSVLESGNHRVSVDETLTIQRKSREIESHLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGG 184
           VLS ALGYSF DSDSLVEQDVG SVAEIFK+YGE++FRE+E E+LRKLSLMNQ V+STGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTL 244
           G VTR +NW YM +GISVWLDVPL+ALVKRISAVGT SRPLLH DSTDAYSKTL  LSTL
Sbjct: 181 GAVTRMINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLIRLSTL 240

Query: 245 LEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF 302
           LE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALEQIE FLK  D YCAF
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

BLAST of MC02g1226 vs. ExPASy TrEMBL
Match: A0A6J1DVB4 (Shikimate kinase OS=Momordica charantia OX=3673 GN=LOC111024387 PE=3 SV=1)

HSP 1 Score: 595 bits (1535), Expect = 8.27e-215
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH 60
           MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH
Sbjct: 1   MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH 60

Query: 61  HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT 120
           HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT
Sbjct: 61  HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT 120

Query: 121 TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 180
           TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV
Sbjct: 121 TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 180

Query: 181 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 240
           STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL
Sbjct: 181 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 240

Query: 241 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC 300
           LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC
Sbjct: 241 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC 300

Query: 301 AF 302
           AF
Sbjct: 301 AF 302

BLAST of MC02g1226 vs. ExPASy TrEMBL
Match: A0A6J1I3Z2 (Shikimate kinase OS=Cucurbita maxima OX=3661 GN=LOC111470366 PE=3 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 1.83e-169
Identity = 244/298 (81.88%), Postives = 264/298 (88.59%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEAT+TQQLQFSPWI+SK IAAKPKGSLHFSRRL EQ MLQAPFSFNLQ SR  +HHRT 
Sbjct: 1   MEATLTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRTV 60

Query: 65  ALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGK 124
           ALKISCSYKNS+ LESGNH   VDE+ T+QRK+REIE YLNG CIY VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYKNSSVLESGNHRVPVDETFTIQRKSREIESYLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGG 184
           VLS ALGYSF DSDSLVEQDVG SVAEIFK+YGE++FRE+E E+LRKLSLMNQ V+STGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTL 244
           G VTRT+NW YM +GISVWLDVPL+ALVKRISAVGT SRPLLH DSTDAYSKTL  LSTL
Sbjct: 181 GAVTRTINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLVRLSTL 240

Query: 245 LEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF 302
           LE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALEQIE FLK  D YCAF
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

BLAST of MC02g1226 vs. ExPASy TrEMBL
Match: A0A6J1EQI5 (Shikimate kinase OS=Cucurbita moschata OX=3662 GN=LOC111434938 PE=3 SV=1)

HSP 1 Score: 476 bits (1224), Expect = 1.74e-167
Identity = 242/298 (81.21%), Postives = 263/298 (88.26%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEATVTQQLQFSPWI+SK IAAKPKGSLHFSRRL EQ MLQAPFSFNLQ SR  +HHR  
Sbjct: 1   MEATVTQQLQFSPWIHSKSIAAKPKGSLHFSRRLEEQHMLQAPFSFNLQLSRRSSHHRIV 60

Query: 65  ALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTVGK 124
           ALKISCSY+NS+ LESGNH   VDE+LT+QRK+REIE +LNG CIY VGMMGSGKTTVGK
Sbjct: 61  ALKISCSYQNSSVLESGNHRVSVDETLTIQRKSREIESHLNGRCIYFVGMMGSGKTTVGK 120

Query: 125 VLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVSTGG 184
           VLS ALGYSF DSDSLVEQDVG SVAEIFK+YGE++FRE+E E+LRKLSLMNQ V+STGG
Sbjct: 121 VLSNALGYSFSDSDSLVEQDVGISVAEIFKVYGEDFFRERETEALRKLSLMNQFVISTGG 180

Query: 185 GVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALLSTL 244
           G VTR +NW YM +GISVWLDVPL+ALVKRISAVGT SRPLLH DSTDAYSKTL  LSTL
Sbjct: 181 GAVTRMINWKYMREGISVWLDVPLEALVKRISAVGTNSRPLLHNDSTDAYSKTLIRLSTL 240

Query: 245 LEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYCAF 302
           LE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALEQIE FLK  D YCAF
Sbjct: 241 LEERGEAYANAEVRVSCEKIAAKLGTKDVSNVTPMAIAIEALEQIEIFLKREDGYCAF 298

BLAST of MC02g1226 vs. ExPASy TrEMBL
Match: A0A0A0LJX8 (Shikimate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G296130 PE=3 SV=1)

HSP 1 Score: 468 bits (1205), Expect = 1.57e-164
Identity = 236/302 (78.15%), Postives = 262/302 (86.75%), Query Frame = 0

Query: 1   MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH 60
           M  SME TVTQQLQFSPWI+SK I+AK KGSLHF RRL EQ +LQAPFSF+L+ SR  NH
Sbjct: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60

Query: 61  HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT 120
            RT ALKISCSY NS+ LESGNH A VDE+L +QRK+REIE YLNG CIYLVGMMGSGKT
Sbjct: 61  RRTVALKISCSYNNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 180
           TVGKVLS ALGYSF DSDSLVEQD+G SVAE+FK+YGE++FRE+E E+LRKLSLM Q V+
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDMGISVAEVFKVYGEDFFRERETEALRKLSLMRQFVI 180

Query: 181 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 240
           STGGG VTR++NW YM KGISVWLDVPL+ALVKRISAVGT SRPLLH DS DAYSKTL  
Sbjct: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVR 240

Query: 241 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC 300
           LSTLLE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALE+IE+FLK  D YC
Sbjct: 241 LSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYC 300

Query: 301 AF 302
           AF
Sbjct: 301 AF 302

BLAST of MC02g1226 vs. ExPASy TrEMBL
Match: E7BBT4 (Shikimate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 466 bits (1198), Expect = 1.83e-163
Identity = 235/302 (77.81%), Postives = 261/302 (86.42%), Query Frame = 0

Query: 1   MQASMEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNH 60
           M  SME TVTQQLQFSPWI+SK I+AK KGSLHF RRL EQ +LQAPFSF+L+ SR  NH
Sbjct: 1   MPVSMETTVTQQLQFSPWIHSKSISAKSKGSLHFPRRLAEQDVLQAPFSFDLRVSRHSNH 60

Query: 61  HRTAALKISCSYKNSTALESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKT 120
            RT ALKISCSY NS+ LESGNH A VDE+L +QRK+REIE YLNG CIYLVGMMGSGKT
Sbjct: 61  RRTVALKISCSYNNSSVLESGNHCASVDETLAIQRKSREIESYLNGRCIYLVGMMGSGKT 120

Query: 121 TVGKVLSKALGYSFCDSDSLVEQDVGTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 180
           TVGKVLS ALGYSF DSDSLVEQD+G SVAE+FK+YGE++FRE+E E+L KLSLM Q V+
Sbjct: 121 TVGKVLSNALGYSFSDSDSLVEQDMGISVAEVFKVYGEDFFRERETEALGKLSLMRQFVI 180

Query: 181 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 240
           STGGG VTR++NW YM KGISVWLDVPL+ALVKRISAVGT SRPLLH DS DAYSKTL  
Sbjct: 181 STGGGAVTRSINWKYMHKGISVWLDVPLEALVKRISAVGTNSRPLLHHDSNDAYSKTLVR 240

Query: 241 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRDDYC 300
           LSTLLE+RGEAYA+A V+VSCEKIAAKLG KD SNVTPMAIA+EALE+IE+FLK  D YC
Sbjct: 241 LSTLLEERGEAYANAEVKVSCEKIAAKLGTKDVSNVTPMAIAIEALEEIETFLKREDGYC 300

Query: 301 AF 302
           AF
Sbjct: 301 AF 302

BLAST of MC02g1226 vs. TAIR 10
Match: AT2G21940.1 (shikimate kinase 1 )

HSP 1 Score: 294.7 bits (753), Expect = 8.7e-80
Identity = 157/293 (53.58%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKP-KGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRT 64
           MEA +TQ++Q+  W++ +K+  KP +GSL +S+++   +  +      LQP R  +  R 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  AALKISCSYKNSTA-LESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTV 124
            +  +SCS  NS+A LE+G+ + F DE + L+RKA E++PYLNG  +YLVGMMGSGKTTV
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPF-DEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTV 120

Query: 125 GKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVS 184
           GK++SK LGY+F D D+L+EQ + GTSVAEIF  +GE +FR KE ++L+KLS   Q+VVS
Sbjct: 121 GKLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVS 180

Query: 185 TGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALL 244
           TGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT+SRPLLH +S DAYS     L
Sbjct: 181 TGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRL 240

Query: 245 STLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLK 295
           S + ++RGEAY +A+ +VS E IAAK G K+ S++TP  IA+EA EQ+ SFL+
Sbjct: 241 SAIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIEAFEQVLSFLE 291

BLAST of MC02g1226 vs. TAIR 10
Match: AT2G21940.2 (shikimate kinase 1 )

HSP 1 Score: 294.7 bits (753), Expect = 8.7e-80
Identity = 157/293 (53.58%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKP-KGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRT 64
           MEA +TQ++Q+  W++ +K+  KP +GSL +S+++   +  +      LQP R  +  R 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  AALKISCSYKNSTA-LESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTV 124
            +  +SCS  NS+A LE+G+ + F DE + L+RKA E++PYLNG  +YLVGMMGSGKTTV
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPF-DEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTV 120

Query: 125 GKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVS 184
           GK++SK LGY+F D D+L+EQ + GTSVAEIF  +GE +FR KE ++L+KLS   Q+VVS
Sbjct: 121 GKLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVS 180

Query: 185 TGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALL 244
           TGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT+SRPLLH +S DAYS     L
Sbjct: 181 TGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRL 240

Query: 245 STLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLK 295
           S + ++RGEAY +A+ +VS E IAAK G K+ S++TP  IA+EA EQ+ SFL+
Sbjct: 241 SAIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIEAFEQVLSFLE 291

BLAST of MC02g1226 vs. TAIR 10
Match: AT2G21940.5 (shikimate kinase 1 )

HSP 1 Score: 294.7 bits (753), Expect = 8.7e-80
Identity = 157/293 (53.58%), Postives = 214/293 (73.04%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKP-KGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRT 64
           MEA +TQ++Q+  W++ +K+  KP +GSL +S+++   +  +      LQP R  +  R 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  AALKISCSYKNSTA-LESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTTV 124
            +  +SCS  NS+A LE+G+ + F DE + L+RKA E++PYLNG  +YLVGMMGSGKTTV
Sbjct: 61  VSPAVSCSDNNSSALLETGSVYPF-DEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTTV 120

Query: 125 GKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVVS 184
           GK++SK LGY+F D D+L+EQ + GTSVAEIF  +GE +FR KE ++L+KLS   Q+VVS
Sbjct: 121 GKLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVVS 180

Query: 185 TGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLALL 244
           TGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT+SRPLLH +S DAYS     L
Sbjct: 181 TGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKRL 240

Query: 245 STLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLK 295
           S + ++RGEAY +A+ +VS E IAAK G K+ S++TP  IA+EA EQ+ SFL+
Sbjct: 241 SAIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIEAFEQVLSFLE 291

BLAST of MC02g1226 vs. TAIR 10
Match: AT2G21940.4 (shikimate kinase 1 )

HSP 1 Score: 292.7 bits (748), Expect = 3.3e-79
Identity = 156/294 (53.06%), Postives = 213/294 (72.45%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKP-KGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRT 64
           MEA +TQ++Q+  W++ +K+  KP +GSL +S+++   +  +      LQP R  +  R 
Sbjct: 1   MEAAITQRIQYPSWVDCRKVECKPQRGSLRYSQQVKVDRRFRGLSLARLQPERRNDQRRA 60

Query: 65  AALKISCSYKNSTA--LESGNHFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTT 124
            +  +SCS  NS+   LE+G+ + F DE + L+RKA E++PYLNG  +YLVGMMGSGKTT
Sbjct: 61  VSPAVSCSDNNSSGALLETGSVYPF-DEDI-LKRKAEEVKPYLNGRSMYLVGMMGSGKTT 120

Query: 125 VGKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLMNQLVV 184
           VGK++SK LGY+F D D+L+EQ + GTSVAEIF  +GE +FR KE ++L+KLS   Q+VV
Sbjct: 121 VGKLMSKVLGYTFFDCDTLIEQAMNGTSVAEIFVHHGENFFRGKETDALKKLSSRYQVVV 180

Query: 185 STGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQDSTDAYSKTLAL 244
           STGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT+SRPLLH +S DAYS     
Sbjct: 181 STGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTDSRPLLHDESGDAYSVAFKR 240

Query: 245 LSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLK 295
           LS + ++RGEAY +A+ +VS E IAAK G K+ S++TP  IA+EA EQ+ SFL+
Sbjct: 241 LSAIWDERGEAYTNANARVSLENIAAKRGYKNVSDLTPTEIAIEAFEQVLSFLE 292

BLAST of MC02g1226 vs. TAIR 10
Match: AT4G39540.1 (shikimate kinase 2 )

HSP 1 Score: 276.6 bits (706), Expect = 2.4e-74
Identity = 155/299 (51.84%), Postives = 206/299 (68.90%), Query Frame = 0

Query: 5   MEATVTQQLQFSPWINSKKIAAKPKGSLHFSRRLIEQQMLQAPFSFNLQPSRPPNHHRTA 64
           MEA   Q+ Q+S W + +    KP+GSL ++ + I++       +  L   R    HR  
Sbjct: 1   MEAATVQRFQYSSWNDLRNFEGKPRGSLRYNTQRIKEDKRFRVVALTLDKRR---DHRLR 60

Query: 65  ALKISCSYKNSTA-LESGN--HFAFVDESLTLQRKAREIEPYLNGHCIYLVGMMGSGKTT 124
               S S KNS+A LE+G+  H  F +E   L++KA E++PYLNG  +YLVGMMGSGKTT
Sbjct: 61  ----SVSDKNSSALLETGSLLHSPFDEEQQILKKKAEEVKPYLNGRSMYLVGMMGSGKTT 120

Query: 125 VGKVLSKALGYSFCDSDSLVEQDV-GTSVAEIFKLYGEEYFREKEMESLRKLSLM-NQLV 184
           VGK+++++LGY+F D D+L+EQ + GTSVAEIF+ +GE  FREKE E+L+KLSLM +Q+V
Sbjct: 121 VGKIMARSLGYTFFDCDTLIEQAMKGTSVAEIFEHFGESVFREKETEALKKLSLMYHQVV 180

Query: 185 VSTGGGVVTRTMNWTYMDKGISVWLDVPLKALVKRISAVGTESRPLLHQD-STDAYSKTL 244
           VSTGGG V R +NW YM KGIS+WLDVPL+AL  RI+AVGT SRPLLH D S D Y+  L
Sbjct: 181 VSTGGGAVIRPINWKYMHKGISIWLDVPLEALAHRIAAVGTGSRPLLHDDESGDTYTAAL 240

Query: 245 ALLSTLLEKRGEAYASAHVQVSCEKIAAKLGNKDASNVTPMAIAMEALEQIESFLKGRD 298
             LST+ + RGEAY  A  +VS E I  KLG +  S++TP  IA+EA EQ++S+L+  D
Sbjct: 241 NRLSTIWDARGEAYTKASARVSLENITLKLGYRSVSDLTPAEIAIEAFEQVQSYLEKED 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q004974.5e-8155.63Shikimate kinase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=SK PE=1 SV=1[more]
Q9SJ051.2e-7853.58Shikimate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK1 PE=1 SV... [more]
Q8GY883.4e-7351.84Shikimate kinase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SK2 PE=1 SV... [more]
Q7X7H93.1e-6657.14Shikimate kinase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK... [more]
Q5NTH42.5e-6358.72Shikimate kinase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SK... [more]
Match NameE-valueIdentityDescription
XP_022157752.11.71e-214100.00shikimate kinase, chloroplastic-like [Momordica charantia][more]
XP_023512754.11.87e-16982.21shikimate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023512755.... [more]
XP_022971695.13.77e-16981.88shikimate kinase, chloroplastic-like [Cucurbita maxima] >XP_022971696.1 shikimat... [more]
KAG7011153.14.39e-16881.54Shikimate kinase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022928030.13.60e-16781.21shikimate kinase, chloroplastic-like [Cucurbita moschata] >XP_022928031.1 shikim... [more]
Match NameE-valueIdentityDescription
A0A6J1DVB48.27e-215100.00Shikimate kinase OS=Momordica charantia OX=3673 GN=LOC111024387 PE=3 SV=1[more]
A0A6J1I3Z21.83e-16981.88Shikimate kinase OS=Cucurbita maxima OX=3661 GN=LOC111470366 PE=3 SV=1[more]
A0A6J1EQI51.74e-16781.21Shikimate kinase OS=Cucurbita moschata OX=3662 GN=LOC111434938 PE=3 SV=1[more]
A0A0A0LJX81.57e-16478.15Shikimate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G296130 PE=3 SV=1[more]
E7BBT41.83e-16377.81Shikimate kinase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G21940.18.7e-8053.58shikimate kinase 1 [more]
AT2G21940.28.7e-8053.58shikimate kinase 1 [more]
AT2G21940.58.7e-8053.58shikimate kinase 1 [more]
AT2G21940.43.3e-7953.06shikimate kinase 1 [more]
AT4G39540.12.4e-7451.84shikimate kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01100SHIKIMTKNASEcoord: 109..124
score: 60.58
coord: 199..216
score: 31.62
coord: 133..146
score: 37.91
coord: 179..188
score: 62.31
coord: 161..169
score: 46.15
NoneNo IPR availablePANTHERPTHR21087:SF20SHIKIMATE KINASE 1, CHLOROPLASTIC-LIKE ISOFORM X1coord: 5..297
NoneNo IPR availablePANTHERPTHR21087SHIKIMATE KINASEcoord: 5..297
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 91..300
e-value: 5.4E-66
score: 224.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 107..233
IPR031322Shikimate kinase/gluconokinasePFAMPF01202SKIcoord: 115..270
e-value: 1.1E-42
score: 145.9
IPR023000Shikimate kinase, conserved sitePROSITEPS01128SHIKIMATE_KINASEcoord: 162..187
IPR000623Shikimate kinase/Threonine synthase-like 1HAMAPMF_00109Shikimate_kinasecoord: 106..290
score: 24.078445
IPR000623Shikimate kinase/Threonine synthase-like 1CDDcd00464SKcoord: 109..268
e-value: 2.85949E-53
score: 169.273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC02g1226.1MC02g1226.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009423 chorismate biosynthetic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0009507 chloroplast
molecular_function GO:0005524 ATP binding
molecular_function GO:0004765 shikimate kinase activity